PI0016321 (gene) Melon (PI 482460) v1

Overview
NamePI0016321
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionDisease resistance protein RGA2-like
Locationchr01: 25981831 .. 25985680 (-)
RNA-Seq ExpressionPI0016321
SyntenyPI0016321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAACCTCTTCTCTTCAACCTTATTGCAGACATCATATTCAAATTGGACTCTTCAGCATTCCGACAGTATGGATCTCTGTGGGGCGGTGTCGAGGATGAATTTGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCTGTTCTTCACGACGCAGAGGAGAAGCAGTTCAAGAACCATGACGTCAAGTCTTGGGAATTTCAAGGCTTAAGTATGTTTTGCACGAGATTGATGACTTGGTGGACGAAGTTTCTTACAAAGTCATTAAAACACAAGTTAAGTCTGGAGATGGAAGAAGCATCCAAGAGGTAAGTAACGCCTGTTCAAAATGTTTTCCAGTTCCATTTTGTCGGAAAGAGAGTCAGCAAATCATGGAAATTAGGCAGAGGCTAGAATGTATTAATAATGATAAAGATCAATTTAGATTTTCTAAGCATGTGATTGAAAGAACAGATGGTTTTGAGTTGAGAATGAGACGGGAGACTCACTCTTTTAAACCTAAAGAGGTAGTGATTGGTAGGGATGATGACAAGGAAGCAGTCATAGATCTTCTACTAATTCCAACACCAAAGAGGATATTGCAATAGTTTCCATTGTTGGAATGGGAGGATTGGGAAAGACTGTCCTTGCTCAAACTATTTATAACCATGAGAGGATAAAGACTCAATTTCAGTTGAAACTATGGGTGCATGTTTATGACAAATTTGATCTGAAAATTATTATCCAAAAGATAATAGAGTCTGCAACCAGGAAGAGGCCTGAATCATTCCTTCAAATAGATTCATTACAACGTGAGCTTAGAAAGGAAATTGATGGAAATAACTATTTGCTCGTTATGGATGATGTGTGGAATGAGGACCAAAAACAGGAATGGTTACATCTGAAAAGATTGTTGATGGGTGGTGCAAAGGGTAGTAGGATTTTGATCACAACACGCAGTGAACAAGTTGCAAAAACTTGCGACTCTACTAGCACCCATTATTTACAACTTTTGGATGCGTCCAATTCTTGGTTATGGTTTCAGAAGATGACAGGTTTGGAAGGATGTTCAAATAATCAAGAGGTCGTGTTTGATCAAAAGAATTCAAATTTGATCCAAATCGGCAAGGAGATTGTGTCAACGTTAAACGGTGTTCCGCTCGCAATAATAACCATTGGAATACTTTTAAAAGATAATAAATCAGAACGTTTTTGGTCATCTTTCAAGAAGAACCTTTACAGTTTACTGGGACAAGGACTAGGCATTGAATTAGGCCACCTGCTACCGATTCTTAAACTCAGCTTTGAACACCTCCCAGCTATTTTGAAGCAATGTTTTCTTTATTATTGCACTTTGTTTCCCAAGGGCTTTCAGATTGAAAAGGATGTCTTGATACGACAATGGAGAGCACAAGGTTTCATTCAACCAAAAAACAACGATAACTTCATTGATATTGGTGAGAAGTATCTTTTGGACTTATTATCAAGGTCATTTTTTGAGGAGGTTCTAACAAATGATTCGGGAGACATAACAGCATATAGGATGCATGATTTGATGCATGATCTTGGTTGTTGGATAATGAATAATGAATGCATGCATGTATGGATGGAGAATGCCATGGATAAAATTAGAATTAATCACTCTTCATTGGAAGAATATAATATTGAAGGATTTGTCGATAACTTAAAGGACAGGAAGTTGATAAGAACATTGATTTGGCGAAATGATTACAATGACAGAGAAGTTCTTTATGAGAAAATCTTTCATAATCTATTGAGATTAAGGACGTTGGAGTTGGACTTGAGCAAAGTAAGTCCAAAACTTGTCCAGTCTATTGGTAGATTGAAACATTTGAGATATTTGGATATTTGTGTGAGTGAAGATATCGAGTATCTTCCGCATTCTATTACTGATTTGTATTTTTTAGAAACACTTATCTTGAGGGATTGCACATTTTTGAAAAACTTGCCAAGAGATATAAAAAATTGGAAGAACCTCACGTATTTTGATCTTTCTTGGAATGTGAAAATGGTAATCCTTCCAAATTCTATTACTGAATTGGATAAGTTGGAAACACTTATCCTCAAGGGTTGGAAAGAATTAAAAGAATTACCAAGAGATATCAAAAATTGAAGAACCTCATGTATCTTGATCTTTCTTCCAATATAGAAATAGAATTCCTTCCAGATTGTATTACTGAGTTGCATAATTTGGAAACGCTTATCCTCAATTCTTGTTCAAAATTAAAAGAATTGCCAAGAAGTACTAAAAACTGGATCAAGCTTAAGCATCTTGACATGCATGGATGCACATCATTGACTCATATGCCAAGAGGACTATGTGGGTTGACTAATCTTCGAACAATGAATTTATTTGTTCTAGGAAAGGATTATGGGAGTGGGTTAAATGAGTTGAACGGACTTGGAAACTTGAGAGGATCATTAACTGTTAAAGGACTGCAATTTTGTACAACTGTTGATGTGCAAGAGAATGCTAAGTACTTGCGACAAAAGTCAGGTCTTCAAGAGTTGAAGTTATATTGGAACATTGTTCTCAACGATCAGCCTACAATCAATAACTACGAAAGAGTATTAAAATGCTTTCAGCCACCTTCAAATCTTCAAGAGATATTGATACACGGATATAATGGGGTGGAGTTATGTGATTGGCTATCCTCTAATTCTCTTGGTCGTCTGGTCAGCATTGAGCTCTCTTCTTGCAGGAAACTACAGCATCTCCCACATTTTGATCAATTACCTTACCTCAAGCGTCTTCATCTTGAAAACTTACCGTGTATCGAATACATTATTGACAACAATGATTTTGTTGTCTTTCCATCCTTGGAGAAATTAAGCATTGTTAAGATGAGCAAGTTAAAGAAATGGTGGAGGATTTCTGGTTTAGCATCTCAAGAAAACTATGACATGTTTCCAATAATATTTCCTTGCCTTTCTGAATTGAAAATTTCTGGTTCTGCTTCAAATCCAGTCAACATGGACGAATATGATGTGCCATGGGAAAGTTTTGAAAGCCTCACCTCTCTGACACTTTGCTACATTTCCAAATTGGAATACATGTCCAAGTATTGGCAGCATACAACTCTTCAACACTTTTCACTACAATTCTGTTTAGATTTAACGAATCTACCTGAATGGATTGTCAACCACACTCAACTCTCAAATTTATGGATTGATAGTTGTCCAAATTTAAGGAGCCTACCAGAATCAATGGCCAATCTTACTTCACTCACGGAATTGAAGATTTGTTCTTGTGACTAACTTCCTTGCCCCGACGGATTCACGGCCTCCCCTGTTTGAAGTCATTGAAAATTTGGTGGTGTCCCAAATTGAAGGAAAGCTGCGAAAGAGAAGATTGGCCCAACGTAGATTTTGTGGGATCAGCAGGACTTAATTAGGTAATATGCAACTTTCTTTAATCCCATTATTCATATATCTCTTTCAAATTCAATTATTTTCTTCAATTAATCCTGAGGTAATTGAATTTGAAATTAAAGTTCATGTGTAGCTCTCACTTCTCAGGTTTACAATTAAAGTTTTATGCATAAAAAAATCAGCATTAATCTCTATGTTTCTTTGATTGCTTTTTGACCAGAAGCCAAGCCCAAAACTTGGAAGCAGATCATTCACCATTAATTGCTTTTACATGTATGGTTTGGTCTTTTTTTTTTTTTTTTGGTTTGTCCATTTCTGTTGAGTCTAATTTACTTATTTCTACCTGATTTACTTAATGAGTAATGTCTCATTGATTTCTCCATTCTCCAGGACTTGCAAAAATTTGGATTTGAAGAGTTGGCTTATCATTTTCATAGTAGTCGTCCTAATATTATTCTTCCTTTTTATCCCTTAA

mRNA sequence

ATGGCTCAACCTCTTCTCTTCAACCTTATTGCAGACATCATATTCAAATTGGACTCTTCAGCATTCCGACAGTATGGATCTCTGTGGGGCGGTGTCGAGGATGAATTTGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCTGTTCTTCACGACGCAGAGGAGAAGCAGTTCAAGAACCATGACGTCAAGTCTTGGGAATTTCAAGGCTTAAGTATGTTTTGCACGAGATTGATGACTTGGTGGACGAAGTTTCTTACAAAGTCATTAAAACACAAGTTAAGTCTGGAGATGGAAGAAGCATCCAAGAGTCATAGATCTTCTACTAATTCCAACACCAAAGAGGATATTGCAATAGTTTCCATTGTTGGAATGGGAGGATTGGGAAAGACTGTCCTTGCTCAAACTATTTATAACCATGAGAGGATAAAGACTCAATTTCAGTTGAAACTATGGGTGCATGTTTATGACAAATTTGATCTGAAAATTATTATCCAAAAGATAATAGAGTCTGCAACCAGGAAGAGGCCTGAATCATTCCTTCAAATAGATTCATTACAACGTGAGCTTAGAAAGGAAATTGATGGAAATAACTATTTGCTCGTTATGGATGATGTGTGGAATGAGGACCAAAAACAGGAATGGTTACATCTGAAAAGATTGTTGATGGGTGGTGCAAAGGGTAGTAGGATTTTGATCACAACACGCAGTGAACAAGTTGCAAAAACTTGCGACTCTACTAGCACCCATTATTTACAACTTTTGGATGCGTCCAATTCTTGGTTATGGTTTCAGAAGATGACAGGTTTGGAAGGATGTTCAAATAATCAAGAGGTCGTGTTTGATCAAAAGAATTCAAATTTGATCCAAATCGGCAAGGAGATTGTGTCAACGTTAAACGGTGTTCCGCTCGCAATAATAACCATTGGAATACTTTTAAAAGATAATAAATCAGAACGTTTTTGGTCATCTTTCAAGAAGAACCTTTACAGTTTACTGGGACAAGGACTAGGCATTGAATTAGGCCACCTGCTACCGATTCTTAAACTCAGCTTTGAACACCTCCCAGCTATTTTGAAGCAATGTTTTCTTTATTATTGCACTTTGTTTCCCAAGGGCTTTCAGATTGAAAAGGATGTCTTGATACGACAATGGAGAGCACAAGAAATAGAATTCCTTCCAGATTGTATTACTGAGTTGCATAATTTGGAAACGCTTATCCTCAATTCTTGTTCAAAATTAAAAGAATTGCCAAGAAGTACTAAAAACTGGATCAAGCTTAAGCATCTTGACATGCATGGATGCACATCATTGACTCATATGCCAAGAGGACTATGTGGGTTGACTAATCTTCGAACAATGAATTTATTTGTTCTAGGAAAGGATTATGGGAGTGGGTTAAATGAGTTGAACGGACTTGGAAACTTGAGAGGATCATTAACTGTTAAAGGACTGCAATTTTGTACAACTGTTGATGTGCAAGAGAATGCTAAGTACTTGCGACAAAAGTCAGGTCTTCAAGAGTTGAAGTTATATTGGAACATTGTTCTCAACGATCAGCCTACAATCAATAACTACGAAAGAGTATTAAAATGCTTTCAGCCACCTTCAAATCTTCAAGAGATATTGATACACGGATATAATGGGGTGGAGTTATGTGATTGGCTATCCTCTAATTCTCTTGGTCGTCTGGTCAGCATTGAGCTCTCTTCTTGCAGGAAACTACAGCATCTCCCACATTTTGATCAATTACCTTACCTCAAGCGTCTTCATCTTGAAAACTTACCGTGTATCGAATACATTATTGACAACAATGATTTTGTTGTCTTTCCATCCTTGGAGAAATTAAGCATTGTTAAGATGAGCAAGTTAAAGAAATGGTGGAGGATTTCTGGTTTAGCATCTCAAGAAAACTATGACATGTTTCCAATAATATTTCCTTGCCTTTCTGAATTGAAAATTTCTGGTTCTGCTTCAAATCCAGTCAACATGGACGAATATGATGTGCCATGGGAAAGTTTTGAAAGCCTCACCTCTCTGACACTTTGCTACATTTCCAAATTGGAATACATGTCCAAATTTGTTCTTGTGACTAACTTCCTTGCCCCGACGGATTCACGGCCTCCCCTGTTTGAAGTCATTGAAAATTTGGTGAAGCCAAGCCCAAAACTTGGAAGCAGATCATTCACCATTAATTGCTTTTACATGACTTGCAAAAATTTGGATTTGAAGAGTTGGCTTATCATTTTCATAGTAGTCGTCCTAATATTATTCTTCCTTTTTATCCCTTAA

Coding sequence (CDS)

ATGGCTCAACCTCTTCTCTTCAACCTTATTGCAGACATCATATTCAAATTGGACTCTTCAGCATTCCGACAGTATGGATCTCTGTGGGGCGGTGTCGAGGATGAATTTGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCTGTTCTTCACGACGCAGAGGAGAAGCAGTTCAAGAACCATGACGTCAAGTCTTGGGAATTTCAAGGCTTAAGTATGTTTTGCACGAGATTGATGACTTGGTGGACGAAGTTTCTTACAAAGTCATTAAAACACAAGTTAAGTCTGGAGATGGAAGAAGCATCCAAGAGTCATAGATCTTCTACTAATTCCAACACCAAAGAGGATATTGCAATAGTTTCCATTGTTGGAATGGGAGGATTGGGAAAGACTGTCCTTGCTCAAACTATTTATAACCATGAGAGGATAAAGACTCAATTTCAGTTGAAACTATGGGTGCATGTTTATGACAAATTTGATCTGAAAATTATTATCCAAAAGATAATAGAGTCTGCAACCAGGAAGAGGCCTGAATCATTCCTTCAAATAGATTCATTACAACGTGAGCTTAGAAAGGAAATTGATGGAAATAACTATTTGCTCGTTATGGATGATGTGTGGAATGAGGACCAAAAACAGGAATGGTTACATCTGAAAAGATTGTTGATGGGTGGTGCAAAGGGTAGTAGGATTTTGATCACAACACGCAGTGAACAAGTTGCAAAAACTTGCGACTCTACTAGCACCCATTATTTACAACTTTTGGATGCGTCCAATTCTTGGTTATGGTTTCAGAAGATGACAGGTTTGGAAGGATGTTCAAATAATCAAGAGGTCGTGTTTGATCAAAAGAATTCAAATTTGATCCAAATCGGCAAGGAGATTGTGTCAACGTTAAACGGTGTTCCGCTCGCAATAATAACCATTGGAATACTTTTAAAAGATAATAAATCAGAACGTTTTTGGTCATCTTTCAAGAAGAACCTTTACAGTTTACTGGGACAAGGACTAGGCATTGAATTAGGCCACCTGCTACCGATTCTTAAACTCAGCTTTGAACACCTCCCAGCTATTTTGAAGCAATGTTTTCTTTATTATTGCACTTTGTTTCCCAAGGGCTTTCAGATTGAAAAGGATGTCTTGATACGACAATGGAGAGCACAAGAAATAGAATTCCTTCCAGATTGTATTACTGAGTTGCATAATTTGGAAACGCTTATCCTCAATTCTTGTTCAAAATTAAAAGAATTGCCAAGAAGTACTAAAAACTGGATCAAGCTTAAGCATCTTGACATGCATGGATGCACATCATTGACTCATATGCCAAGAGGACTATGTGGGTTGACTAATCTTCGAACAATGAATTTATTTGTTCTAGGAAAGGATTATGGGAGTGGGTTAAATGAGTTGAACGGACTTGGAAACTTGAGAGGATCATTAACTGTTAAAGGACTGCAATTTTGTACAACTGTTGATGTGCAAGAGAATGCTAAGTACTTGCGACAAAAGTCAGGTCTTCAAGAGTTGAAGTTATATTGGAACATTGTTCTCAACGATCAGCCTACAATCAATAACTACGAAAGAGTATTAAAATGCTTTCAGCCACCTTCAAATCTTCAAGAGATATTGATACACGGATATAATGGGGTGGAGTTATGTGATTGGCTATCCTCTAATTCTCTTGGTCGTCTGGTCAGCATTGAGCTCTCTTCTTGCAGGAAACTACAGCATCTCCCACATTTTGATCAATTACCTTACCTCAAGCGTCTTCATCTTGAAAACTTACCGTGTATCGAATACATTATTGACAACAATGATTTTGTTGTCTTTCCATCCTTGGAGAAATTAAGCATTGTTAAGATGAGCAAGTTAAAGAAATGGTGGAGGATTTCTGGTTTAGCATCTCAAGAAAACTATGACATGTTTCCAATAATATTTCCTTGCCTTTCTGAATTGAAAATTTCTGGTTCTGCTTCAAATCCAGTCAACATGGACGAATATGATGTGCCATGGGAAAGTTTTGAAAGCCTCACCTCTCTGACACTTTGCTACATTTCCAAATTGGAATACATGTCCAAATTTGTTCTTGTGACTAACTTCCTTGCCCCGACGGATTCACGGCCTCCCCTGTTTGAAGTCATTGAAAATTTGGTGAAGCCAAGCCCAAAACTTGGAAGCAGATCATTCACCATTAATTGCTTTTACATGACTTGCAAAAATTTGGATTTGAAGAGTTGGCTTATCATTTTCATAGTAGTCGTCCTAATATTATTCTTCCTTTTTATCCCTTAA

Protein sequence

MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKNHDVKSWEFQGLSMFCTRLMTWWTKFLTKSLKHKLSLEMEEASKSHRSSTNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLMGGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQEIEFLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQENAKYLRQKSGLQELKLYWNIVLNDQPTINNYERVLKCFQPPSNLQEILIHGYNGVELCDWLSSNSLGRLVSIELSSCRKLQHLPHFDQLPYLKRLHLENLPCIEYIIDNNDFVVFPSLEKLSIVKMSKLKKWWRISGLASQENYDMFPIIFPCLSELKISGSASNPVNMDEYDVPWESFESLTSLTLCYISKLEYMSKFVLVTNFLAPTDSRPPLFEVIENLVKPSPKLGSRSFTINCFYMTCKNLDLKSWLIIFIVVVLILFFLFIP
Homology
BLAST of PI0016321 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.0e-69
Identity = 241/861 (27.99%), Postives = 360/861 (41.81%), Query Frame = 0

Query: 31  GVEDEFDKLKHSLSAIQAVLHDAEEKQFKNHDVKSWEFQGLSMFCTRLMTWWTKFLTKS- 90
           G +DEF +L    S IQAVL DA+EKQ  N  +++W  Q L+     +     ++ TK+ 
Sbjct: 26  GFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENW-LQKLNAATYEVDDILDEYKTKAT 85

Query: 91  ----------------LKHKLSLEMEEASKSHRS-------------------------- 150
                            +HK+   M++  K  ++                          
Sbjct: 86  RFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRETGS 145

Query: 151 ---------------------STNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQ 210
                                  N +  + ++++ I+GMGGLGKT LAQ ++N +R+   
Sbjct: 146 VLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEH 205

Query: 211 FQLKLWVHVYDKFDLKIIIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDV 270
           F  K+W+ V + FD K +I+ I+ES   +     + +  LQ++L++ ++G  YLLV+DDV
Sbjct: 206 FHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDV 265

Query: 271 WNEDQKQEWLHLKRLLMGGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQK 330
           WNEDQ Q+W +L+ +L  GA G+ +L TTR E+V     +   + L  L   + WL F  
Sbjct: 266 WNEDQ-QKWANLRAVLKVGASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLF-- 325

Query: 331 MTGLEGCSNNQEVVFDQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK 390
              ++    +QE +    N NL+ IGKEIV    GVPLA  T+G +L   + ER W   +
Sbjct: 326 ---MQRAFGHQEEI----NPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVR 385

Query: 391 KNLYSLLGQGLGIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWR 450
            +    L Q    +   +LP L+LS+  LP  LKQCF  YC +FPK  ++EK+ LI  W 
Sbjct: 386 DSPIWNLPQ----DESSILPALRLSYHQLPLDLKQCFA-YCAVFPKDAKMEKEKLISLWM 445

Query: 451 A-----------------------------QEIEF------------------------- 510
           A                             QEIE                          
Sbjct: 446 AHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFSANT 505

Query: 511 ------------------------------------------------------------ 570
                                                                       
Sbjct: 506 SSSNIREINKHSYTHMMSIGFAEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLV 565

Query: 571 --------------LPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSL 630
                         LP  + +L NL+TL L  C+KL  LP+ T     L++L + G  SL
Sbjct: 566 HLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSL 625

Query: 631 THMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQENAK 685
           T MP  +  LT L+T+  FV+G+  G  L EL  L NL GS+ +  L+     D      
Sbjct: 626 TCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKN-DKDAKEA 685

BLAST of PI0016321 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 9.3e-67
Identity = 231/810 (28.52%), Postives = 341/810 (42.10%), Query Frame = 0

Query: 31  GVEDEFDKLKHSLSAIQAVLHDAEEKQFKNHDVKSWEFQGLSM-------FCTRLMTWWT 90
           G +DEF +L    S IQAVL DA+EKQ  +  +++W  Q L+              T  T
Sbjct: 26  GFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENW-LQKLNAATYEVDDILDEYKTKAT 85

Query: 91  KFLTKS----------LKHKLSLEM-----------EEASKSH--------RSSTNS--- 150
           +FL              +HK+   M           EE  K H        +++T     
Sbjct: 86  RFLQSEYGRYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQEKIIERQAATRETGS 145

Query: 151 ----------------------NTKED---IAIVSIVGMGGLGKTVLAQTIYNHERIKTQ 210
                                 NT  D   ++++ I+GMGGLGKT L+Q ++N +R+  +
Sbjct: 146 VLTEPQVYGRDKEKDEIVKILINTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTER 205

Query: 211 FQLKLWVHVYDKFDLKIIIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDV 270
           F  K+W+ + D F+ K +I+ I+ES   K   S + +  LQ++L++ ++G  Y LV+DDV
Sbjct: 206 FYPKIWICISDDFNEKRLIKAIVESIEGK-SLSDMDLAPLQKKLQELLNGKRYFLVLDDV 265

Query: 271 WNEDQKQEWLHLKRLLMGGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQK 330
           WNEDQ  +W +L+ +L  GA G+ +L TTR E+V     +   + L  L   + W  F  
Sbjct: 266 WNEDQ-HKWANLRAVLKVGASGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLF-- 325

Query: 331 MTGLEGCSNNQEVVFDQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK 390
              ++    +QE +    N NL+ IGKEIV    GVPLA  T+G +L+  + ER W   +
Sbjct: 326 ---MQRAFGHQEEI----NPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVR 385

Query: 391 KNLYSLLGQGLGIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWR 450
            +    L Q    +   +LP L+LS+ HLP  L+QCF+ YC +FPK  ++ K+ LI  W 
Sbjct: 386 DSPIWNLPQ----DESSILPALRLSYHHLPLDLRQCFV-YCAVFPKDTKMAKENLIAFWM 445

Query: 451 A-----------------------------QEIEF------------------------- 510
           A                             QEIE                          
Sbjct: 446 AHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANT 505

Query: 511 ------------------------------------------------------------ 570
                                                                       
Sbjct: 506 SSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLV 565

Query: 571 ---------------LPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTS 630
                          LP  + +L NL+TL L+ C  L  LP+ T     L++L + GC S
Sbjct: 566 HLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGC-S 625

Query: 631 LTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQENA 646
           LT  P  +  LT L++++ FV+GK  G  L EL  L NL GS+++  L      D     
Sbjct: 626 LTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLD-RVKKDTDAKE 685

BLAST of PI0016321 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 9.3e-67
Identity = 233/837 (27.84%), Postives = 349/837 (41.70%), Query Frame = 0

Query: 31  GVEDEFDKLKHSLSAIQAVLHDAEEKQFKNHDVKSW------------------------ 90
           G E E +KL    S IQAVL DA+EKQ K+  +++W                        
Sbjct: 26  GFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIR 85

Query: 91  -EFQGLSMFCTRLMTWWTKF--LTKSLKHKLSLEMEEASKSH--------------RSS- 150
            E   L  +   ++ +  K     K +  KL    EE  K H              R + 
Sbjct: 86  FEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHFLEKITERQAAAATRETG 145

Query: 151 -----------------------TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT 210
                                   N N  E++ +  I+GMGGLGKT LAQ I+N ER+  
Sbjct: 146 FVLTEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTK 205

Query: 211 QFQLKLWVHVYDKFDLKIIIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDD 270
            F  K+WV V D FD K +I+ II +  R  P     + S Q++L++ ++G  YLLV+DD
Sbjct: 206 HFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPH-VEDLASFQKKLQELLNGKRYLLVLDD 265

Query: 271 VWNEDQKQEWLHLKRLLMGGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWF- 330
           VWN+D  ++W  L+ +L  GA+G+ IL TTR E+V     +   ++L  L   +S L F 
Sbjct: 266 VWNDD-LEKWAKLRAVLTVGARGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFM 325

Query: 331 QKMTGLEGCSNNQEVVFDQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSS 390
           Q+  G +           + N NL+ IGKEIV    GVPLA  T+G LL+  + E  W  
Sbjct: 326 QRAFGQQ----------KEANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEH 385

Query: 391 FKKNLYSLLGQGLGIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQ 450
            + N    L Q    +   +LP L+LS+ HLP  L+QCF  YC +FPK  ++ K+ LI  
Sbjct: 386 VRDNEIWSLPQ----DESSILPALRLSYHHLPLDLRQCFA-YCAVFPKDTKMIKENLITL 445

Query: 451 WRA-----------------------------QEIE------------------------ 510
           W A                             QEIE                        
Sbjct: 446 WMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLFSA 505

Query: 511 ------------------------------------------------------------ 570
                                                                       
Sbjct: 506 SASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGD 565

Query: 571 ---------------FLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCT 630
                           LP+ + +L NL+TL +++C  L  LP+ T     L+HL + GC 
Sbjct: 566 LLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC- 625

Query: 631 SLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQ-FCTTVDVQE 654
            LT  P  +  LT L+T+  F++G   G  L EL  L NL GS+++  L+      D + 
Sbjct: 626 PLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDAEA 685

BLAST of PI0016321 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 255.8 bits (652), Expect = 1.6e-66
Identity = 254/908 (27.97%), Postives = 368/908 (40.53%), Query Frame = 0

Query: 31  GVEDEFDKLKHSLSAIQAVLHDAEEKQFKNHDVKSW------------------------ 90
           G E EF KL    S IQAVL DA+EKQ K   +K+W                        
Sbjct: 26  GFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDCKTEAAR 85

Query: 91  -EFQGLSMFCTRLMTWWTKF--LTKSLKHKLSLEMEEASKSHRSS--------------- 150
            +   L  +  R +T+  K     K +  KL    EE    H                  
Sbjct: 86  FKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLDERIIERQAARRQTGFV 145

Query: 151 ---------------------TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQF 210
                                 N +  E++ ++ I+GMGGLGKT LAQ ++N +RI   F
Sbjct: 146 LTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHF 205

Query: 211 QLKLWVHVYDKFDLKIIIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVW 270
            LK+WV V D FD K +I+ I+ES   K     + +  LQ++L++ ++G  Y LV+DDVW
Sbjct: 206 NLKIWVCVSDDFDEKRLIKAIVESIEGKSLGD-MDLAPLQKKLQELLNGKRYFLVLDDVW 265

Query: 271 NEDQKQEWLHLKRLLMGGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKM 330
           NEDQ ++W +L+ +L  GA G+ ILITTR E++     +   + L  L   + WL F++ 
Sbjct: 266 NEDQ-EKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQR 325

Query: 331 TGLEGCSNNQEVVFDQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFKK 390
                C         + +  L++IGKEIV    GVPLA  T+G LL+  + E  W   + 
Sbjct: 326 A---FCHQT------ETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRD 385

Query: 391 NLYSLLGQGLGIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRA 450
           +    L Q    +   +LP L+LS+ HLP  L+QCF  YC +FPK  +IEK+ LI  W A
Sbjct: 386 SEIWNLPQ----DENSVLPALRLSYHHLPLDLRQCFA-YCAVFPKDTKIEKEYLIALWMA 445

Query: 451 -----------------------------QEIEF-------------------------- 510
                                        QEIE                           
Sbjct: 446 HSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASAS 505

Query: 511 ------------------------------------------------------------ 570
                                                                       
Sbjct: 506 SRSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQ 565

Query: 571 -----------------------LPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKH 630
                                  LP  + +L NL+TL L +C  L  LP+ T     L++
Sbjct: 566 LPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRN 625

Query: 631 LDMHGCTSLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCT 690
           L +  C  LT MP  +  LT L+T+  FV+G+  G  L EL  L NLRG++++  L+   
Sbjct: 626 LVLDHC-PLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVK 685

Query: 691 TVDVQENAKYLRQKSGLQELKLYWNIVLNDQPTINNYE----RVLKCFQPPSNLQEILIH 719
             D++     L  K+ L  L + W     D+P  N YE    +VL+  +P  NL+ + I 
Sbjct: 686 N-DMEAKEANLSAKANLHSLSMSW-----DRP--NRYESEEVKVLEALKPHPNLKYLEII 745

BLAST of PI0016321 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 6.0e-58
Identity = 186/688 (27.03%), Postives = 286/688 (41.57%), Query Frame = 0

Query: 117 IAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKIIESATRKR 176
           I +V+IVG+GG+GKT L+Q +YN + +++ F  K+W HV ++FD+  I +K+ ES T  R
Sbjct: 196 ITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVT-SR 255

Query: 177 PESFLQIDSLQRELRKEIDGNN--YLLVMDDVWNEDQKQEWLHLKRLLMGGAKGSRILIT 236
           P  F  +D LQ +L++ + G    +LLV+DD+WNE+   +W  L++  +  A+GS+IL+T
Sbjct: 256 PCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNEN-FADWDLLRQPFIHAAQGSQILVT 315

Query: 237 TRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQKNSNLIQIGKE 296
           TRS++VA    +   H LQ L   + W  F K         NQE      N  +  + + 
Sbjct: 316 TRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKTV-----FGNQEPCL---NREIGDLAER 375

Query: 297 IVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELGHLLPILKLSFEH 356
           IV    G+PLA+ T+G +L+       W    + + S     L  +  +LLP+L++S+ +
Sbjct: 376 IVHKCRGLPLAVKTLGGVLRFEGKVIEW----ERVLSSRIWDLPADKSNLLPVLRVSYYY 435

Query: 357 LPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------------------- 416
           LPA LK+CF  YC++FPKG   EKD ++  W A+                          
Sbjct: 436 LPAHLKRCFA-YCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESR 495

Query: 417 ------------------------------------------------------------ 476
                                                                       
Sbjct: 496 SLLQKTKTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFE 555

Query: 477 ------------------------------------------------------------ 536
                                                                       
Sbjct: 556 ALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKN 615

Query: 537 ------------EIEFLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCT 596
                       E+E LP  +  ++NL+TL+L+ CS LKELP    N I L++LD+ G T
Sbjct: 616 ISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-T 675

Query: 597 SLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQEN 628
            L  MPR    L +L+T+  F +    GS ++EL GL +L G L +  LQ    VDV + 
Sbjct: 676 KLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQ--RVVDVADA 735

BLAST of PI0016321 vs. ExPASy TrEMBL
Match: A0A5D3CF63 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00550 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 2.2e-183
Identity = 399/771 (51.75%), Postives = 465/771 (60.31%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSL------------------EMEEASKSHRSS-------------------- 180
            K ++ +L                    +  E  +  R +                    
Sbjct: 121 IKEIRERLQAINDDKNQFGFCKNVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           +IQKIIESATRK+P+SFLQIDSLQ ELRK+IDG  Y LVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 VIQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSW  FQKMTGLE CSNNQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
            NSN+IQIGK I+S L GVPL I TIG LLKDNKSERFW SFK K LY + GQG    L 
Sbjct: 361 TNSNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQD-ALK 420

Query: 421 HLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------- 480
            +  IL+LS+++LPA LKQCFL YC LFPK ++IEK+ LI  WRAQ              
Sbjct: 421 EIQLILELSYKYLPANLKQCFL-YCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYL 480

Query: 481 ------------------------------------------------EIEFLPDCITEL 540
                                                            IEFLPD ITEL
Sbjct: 481 IDIGDDYFMELLSRCDLIPNVMPWEFIGLPKDIKNCNNLRQLDLSENKSIEFLPDSITEL 540

Query: 541 HNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSLTHMPRGLCGLTNLRTMNLFVLG 600
             LE L+L +C KLKELP+ TK  I LKHLD+ GC +LTHMP+GL  LTNL+TMN FVLG
Sbjct: 541 CKLEALMLQNCRKLKELPKDTKKLINLKHLDLWGCWALTHMPKGLGELTNLQTMNTFVLG 600

Query: 601 KDYGSGLNELNGLGNLRGSLTVKGLQFCTT-VDVQE-NAKYLRQKSGLQELKLYWNIVLN 625
           K+ G  L EL+GL  LRG L ++ L+FCT+ VD Q+   K+L  KS LQ L L W     
Sbjct: 601 KNKGGDLKELDGLTKLRGKLRIEHLEFCTSIVDHQQIKGKFLNLKSSLQRLGLQW----- 660

BLAST of PI0016321 vs. ExPASy TrEMBL
Match: A0A5A7UYL5 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00780 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 3.8e-172
Identity = 402/877 (45.84%), Postives = 465/877 (53.02%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESATRK+P+SFLQIDSLQ ELRK+IDG  Y LVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 IIQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSW  FQKMTGLE CSNNQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
            NSN+IQIGK I+S L GVPL I TIG LLKDNKSERFW SFK K LY + GQG    L 
Sbjct: 361 TNSNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQD-ALK 420

Query: 421 HLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------- 480
            +  IL+LS+++LPA LKQCFL YC LFPK ++IEK+ LI  WRAQ              
Sbjct: 421 EMQLILELSYKYLPANLKQCFL-YCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYL 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 IDIGDDYFMELLSRSFFQEVEKNDFGDIIACKMHDLMHDLACSITNNECVRGLKGNVIDK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 RTRHFLWEEECHEDQLMGPLSKATHLRTFSCKENCVVGYRCNLEETFRNMSRLRTLHLFR 600

Query: 601 ----------------------------------EIEFLPDCITELHNLETLILNSCSKL 625
                                              IEFLPD ITEL  LE L+L +C KL
Sbjct: 601 CDLIPNVKPWEFIGLPKDIKNCNNLRQLDLSENKSIEFLPDSITELCKLEALMLQNCRKL 660

BLAST of PI0016321 vs. ExPASy TrEMBL
Match: A0A5D3CEX2 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00570 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 6.5e-172
Identity = 394/797 (49.44%), Postives = 482/797 (60.48%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+NL ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVL DAEE+Q KN
Sbjct: 1   MAEAILYNLTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLLDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRTRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFILEDEVIGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IY H  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYTHYGMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESAT K+ ESFLQ+DSLQ ELRK+IDG  YLLVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 IIQKIIESATGKKTESFLQLDSLQCELRKQIDGKKYLLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSWL FQKMTGLE   +NQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFVHRLQILDASNSWLLFQKMTGLEERFDNQEVKLDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
           KNSNLI+IG EIVS LNGVPL I  IG LLKDNKSERFW SFK K L+ +L Q     L 
Sbjct: 361 KNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQD-ALK 420

Query: 421 HLLPILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQEIEFLPDCITELH 480
            +  IL+LS+++LP+  LKQCF  YC LFPK ++I+K+ LI  WRAQ   F+        
Sbjct: 421 EMQLILELSYKYLPSSNLKQCF-RYCALFPKDYKIQKNELILLWRAQ--GFIQPNGNNND 480

Query: 481 NLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSLTHMPRGLCGLTNLRTMNLFVLGK 540
           + +   +                + L+     GC  LTHMP+GL  LTNL+TMN FVLGK
Sbjct: 481 DGDDYFMQ---------------LLLRSFFQEGCNDLTHMPKGLGELTNLQTMNTFVLGK 540

Query: 541 DYGSGLNELNGLGNLRGSLTVKGLQFCTT-VDVQENAKYLRQKSGLQELKLYW--NIVLN 600
           + GS L EL+GL  LRG L+++ L FC++ VD Q   K+L+QKS LQ L L W  +++++
Sbjct: 541 NKGSELKELDGLTKLRGELSIRHLAFCSSIVDQQMKGKFLQQKSDLQVLDLNWEKHLIVD 600

Query: 601 DQPTINNYERVLKCFQPPSNLQEILIHGYNGVELCDWLSSNSLGRLVSIELSSCRKLQHL 660
           D+     YE VL C QP SNL++I I+GY GV+LC+W+SS  LG LV++ L  C +L+HL
Sbjct: 601 DELEDVIYESVLDCLQPHSNLRKIYINGYGGVKLCNWVSSKFLGCLVTVRLYHCERLRHL 660

Query: 661 PHFDQLPYLKRLHLENLPCIEYIIDNNDFVV-----FPSLEKLSIVKMSKLKKWWRISGL 692
           P FDQ P LKRL LE LP IEYII NN+  V     FPS+++L I  M KL  W + + L
Sbjct: 661 PRFDQFPNLKRLSLEVLPNIEYIIVNNNDSVSSSTNFPSMKELEISNMPKLVSWCKGTTL 720

BLAST of PI0016321 vs. ExPASy TrEMBL
Match: A0A5A7UTU8 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00660 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 6.5e-156
Identity = 392/973 (40.29%), Postives = 464/973 (47.69%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESAT+K+P+SFLQIDSLQ ELRK+IDG  YLLVMDDVWNE +K+EWL+LK+LLM
Sbjct: 241 IIQKIIESATKKKPKSFLQIDSLQHELRKQIDGKKYLLVMDDVWNE-KKEEWLNLKKLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LD SNSWL FQKMTGLEG   NQE   DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFVHLLQILDTSNSWLLFQKMTGLEGRLINQEAELDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
           KNSNLI+IG EIVS LNGVPL I  IG LLKDNKSERFW SFK K L+ +L Q     L 
Sbjct: 361 KNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQD-ALK 420

Query: 421 HLLPILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ------------- 480
            +  IL+LS+++LP+  LKQCF  YC LFPK ++I+KD LI  WRAQ             
Sbjct: 421 EMQLILELSYKYLPSSNLKQCF-RYCALFPKDYKIQKDELILLWRAQGFIQPNGNNDDDN 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 YLVDIGDDYFMQLLSRSFFQEVEKDALGEIITCKMHDLMHDLACSIANHECVRGLKGNDI 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 DKRTRHFFLEGGSRENQLMGHLSKAKYLRTFFRQDHYGVYSQWKNFKEIIFCNIFRLRAW 600

Query: 601 ------------------------------------------------------------ 628
                                                                       
Sbjct: 601 HSQLIGGIEDVESLEFIAKLKHLRYLRMKNCSVISTDLLDCIMKLYNLETFIFQSRGLKS 660

BLAST of PI0016321 vs. ExPASy TrEMBL
Match: A0A5D3CJK7 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00420 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 1.5e-155
Identity = 391/973 (40.18%), Postives = 464/973 (47.69%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAE++Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEDQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESAT+K+P+SFLQIDSLQ ELRK+IDG  YLLVMDDVWNE +K+EWL+LK+LLM
Sbjct: 241 IIQKIIESATKKKPKSFLQIDSLQHELRKQIDGKKYLLVMDDVWNE-KKEEWLNLKKLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LD SNSWL FQKMTGLEG   NQE   DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFVHLLQILDTSNSWLLFQKMTGLEGRLINQEAELDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
           KNSNLI+IG EIVS LNGVPL I  IG LLKDNKSERFW SFK K L+ +L Q     L 
Sbjct: 361 KNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQD-ALK 420

Query: 421 HLLPILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ------------- 480
            +  IL+LS+++LP+  LKQCF  YC LFPK ++I+KD LI  WRAQ             
Sbjct: 421 EMQLILELSYKYLPSSNLKQCF-RYCALFPKDYKIQKDELILLWRAQGFIQPNGNNDDDN 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 YLVDIGDDYFMQLLSRSFFQEVEKDALGEIITCKMHDLMHDLACSIANHECVRGLKGNDI 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 DKRTRHFFLEGGSRENQLMGHLSKAKYLRTFFRQDHYGVYSQWKNFKEIIFCNIFRLRAW 600

Query: 601 ------------------------------------------------------------ 628
                                                                       
Sbjct: 601 HSQLIGGIEDVESLEFIAKLKHLRYLRMKNCSVISTDLLDCIMKLYNLETFIFQSRGLKS 660

BLAST of PI0016321 vs. NCBI nr
Match: TYK10411.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 652.5 bits (1682), Expect = 4.4e-183
Identity = 399/771 (51.75%), Postives = 465/771 (60.31%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSL------------------EMEEASKSHRSS-------------------- 180
            K ++ +L                    +  E  +  R +                    
Sbjct: 121 IKEIRERLQAINDDKNQFGFCKNVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           +IQKIIESATRK+P+SFLQIDSLQ ELRK+IDG  Y LVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 VIQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSW  FQKMTGLE CSNNQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
            NSN+IQIGK I+S L GVPL I TIG LLKDNKSERFW SFK K LY + GQG    L 
Sbjct: 361 TNSNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQD-ALK 420

Query: 421 HLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------- 480
            +  IL+LS+++LPA LKQCFL YC LFPK ++IEK+ LI  WRAQ              
Sbjct: 421 EIQLILELSYKYLPANLKQCFL-YCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYL 480

Query: 481 ------------------------------------------------EIEFLPDCITEL 540
                                                            IEFLPD ITEL
Sbjct: 481 IDIGDDYFMELLSRCDLIPNVMPWEFIGLPKDIKNCNNLRQLDLSENKSIEFLPDSITEL 540

Query: 541 HNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSLTHMPRGLCGLTNLRTMNLFVLG 600
             LE L+L +C KLKELP+ TK  I LKHLD+ GC +LTHMP+GL  LTNL+TMN FVLG
Sbjct: 541 CKLEALMLQNCRKLKELPKDTKKLINLKHLDLWGCWALTHMPKGLGELTNLQTMNTFVLG 600

Query: 601 KDYGSGLNELNGLGNLRGSLTVKGLQFCTT-VDVQE-NAKYLRQKSGLQELKLYWNIVLN 625
           K+ G  L EL+GL  LRG L ++ L+FCT+ VD Q+   K+L  KS LQ L L W     
Sbjct: 601 KNKGGDLKELDGLTKLRGKLRIEHLEFCTSIVDHQQIKGKFLNLKSSLQRLGLQW----- 660

BLAST of PI0016321 vs. NCBI nr
Match: XP_031736581.1 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothetical protein Csa_006537 [Cucumis sativus])

HSP 1 Score: 619.4 bits (1596), Expect = 4.2e-173
Identity = 426/999 (42.64%), Postives = 517/999 (51.75%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +LFNL ADIIFKL SSA RQ+GSL GGV+D+FDKL HSLSAIQAVLHDAEEKQFK+
Sbjct: 1   MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60

Query: 61  HDVKSW----------------EF-------QGLSMFCTRLMTWWTKFLTK-SLKHKLSL 120
           H V+ W                EF       Q L     ++ T ++KF+T   + HK+  
Sbjct: 61  HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVLQSNRKQVRTLFSKFITNWKIGHKIKE 120

Query: 121 --------------------------EMEEASKSHRSS---------------------- 180
                                     + +E  +  R +                      
Sbjct: 121 ISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDDKEAVIDL 180

Query: 181 -TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQK 240
             NSNTKEDIAIVSIVGM G GKT LAQ IYNH+RI TQFQLK+WV V D+FDLKI IQK
Sbjct: 181 LLNSNTKEDIAIVSIVGMPGFGKTALAQFIYNHKRIMTQFQLKIWVCVSDEFDLKITIQK 240

Query: 241 IIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLMGGAK 300
           IIESAT K+P+S LQ+D LQ ELRK+IDG  YL+VMDDVWNE +K++WLHLKRLLMGGAK
Sbjct: 241 IIESATGKKPKSLLQMDPLQCELRKQIDGKKYLIVMDDVWNE-KKEKWLHLKRLLMGGAK 300

Query: 301 GSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQKNSN 360
           GSRILITTRSEQVAKT DST  H LQ+LDASNSWL FQKM GLE  SNNQE+  DQKNSN
Sbjct: 301 GSRILITTRSEQVAKTFDSTFVHLLQILDASNSWLLFQKMIGLEEHSNNQEIELDQKNSN 360

Query: 361 LIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELGHLLP 420
           LIQIG EIVSTL GVPL I TIG LLKDNKSERFW SFK K LY +LG+G    L  +  
Sbjct: 361 LIQIGMEIVSTLRGVPLLIRTIGGLLKDNKSERFWLSFKNKELYQVLGRGQD-ALKEIQL 420

Query: 421 ILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ----------------- 480
            L+LS+++LP+  LKQCFL YC LFPK ++I+KD LI  WRAQ                 
Sbjct: 421 FLELSYKYLPSSNLKQCFL-YCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSSLVD 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 IGEDYFMELLSRSFFQEVEKNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVIDKRT 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 HHLSFEKVSHEDQLMGSLSKATHLRTLFIQDVCSRCNLEETFHNIFQLRTLHLNLYSPTK 600

Query: 601 ------------------------------------------------------------ 660
                                                                       
Sbjct: 601 FAKTWKFISKLKHLRYLHLKNSFCVTYLPDSILELYNLETFIFQSSLLKKLPSNVGNLIN 660

Query: 661 ----------EIEFLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSL 692
                      +EFLPD IT+L+ LE LIL+ CS LKELP+ TK  I LK L ++GC++L
Sbjct: 661 LKHLDLSSHLNLEFLPDSITKLYKLEALILHGCSNLKELPKYTKRLINLKSLVLYGCSAL 720

BLAST of PI0016321 vs. NCBI nr
Match: KAA0058609.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 615.1 bits (1585), Expect = 7.9e-172
Identity = 402/877 (45.84%), Postives = 465/877 (53.02%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESATRK+P+SFLQIDSLQ ELRK+IDG  Y LVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 IIQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSW  FQKMTGLE CSNNQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
            NSN+IQIGK I+S L GVPL I TIG LLKDNKSERFW SFK K LY + GQG    L 
Sbjct: 361 TNSNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQD-ALK 420

Query: 421 HLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------- 480
            +  IL+LS+++LPA LKQCFL YC LFPK ++IEK+ LI  WRAQ              
Sbjct: 421 EMQLILELSYKYLPANLKQCFL-YCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYL 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 IDIGDDYFMELLSRSFFQEVEKNDFGDIIACKMHDLMHDLACSITNNECVRGLKGNVIDK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 RTRHFLWEEECHEDQLMGPLSKATHLRTFSCKENCVVGYRCNLEETFRNMSRLRTLHLFR 600

Query: 601 ----------------------------------EIEFLPDCITELHNLETLILNSCSKL 625
                                              IEFLPD ITEL  LE L+L +C KL
Sbjct: 601 CDLIPNVKPWEFIGLPKDIKNCNNLRQLDLSENKSIEFLPDSITELCKLEALMLQNCRKL 660

BLAST of PI0016321 vs. NCBI nr
Match: TYK10413.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 614.4 bits (1583), Expect = 1.3e-171
Identity = 394/797 (49.44%), Postives = 482/797 (60.48%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+NL ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVL DAEE+Q KN
Sbjct: 1   MAEAILYNLTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLLDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRTRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFILEDEVIGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IY H  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYTHYGMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESAT K+ ESFLQ+DSLQ ELRK+IDG  YLLVMDDVWNE +K+EWLHLKRLLM
Sbjct: 241 IIQKIIESATGKKTESFLQLDSLQCELRKQIDGKKYLLVMDDVWNE-KKEEWLHLKRLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LDASNSWL FQKMTGLE   +NQEV  DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFVHRLQILDASNSWLLFQKMTGLEERFDNQEVKLDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
           KNSNLI+IG EIVS LNGVPL I  IG LLKDNKSERFW SFK K L+ +L Q     L 
Sbjct: 361 KNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQD-ALK 420

Query: 421 HLLPILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQEIEFLPDCITELH 480
            +  IL+LS+++LP+  LKQCF  YC LFPK ++I+K+ LI  WRAQ   F+        
Sbjct: 421 EMQLILELSYKYLPSSNLKQCF-RYCALFPKDYKIQKNELILLWRAQ--GFIQPNGNNND 480

Query: 481 NLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCTSLTHMPRGLCGLTNLRTMNLFVLGK 540
           + +   +                + L+     GC  LTHMP+GL  LTNL+TMN FVLGK
Sbjct: 481 DGDDYFMQ---------------LLLRSFFQEGCNDLTHMPKGLGELTNLQTMNTFVLGK 540

Query: 541 DYGSGLNELNGLGNLRGSLTVKGLQFCTT-VDVQENAKYLRQKSGLQELKLYW--NIVLN 600
           + GS L EL+GL  LRG L+++ L FC++ VD Q   K+L+QKS LQ L L W  +++++
Sbjct: 541 NKGSELKELDGLTKLRGELSIRHLAFCSSIVDQQMKGKFLQQKSDLQVLDLNWEKHLIVD 600

Query: 601 DQPTINNYERVLKCFQPPSNLQEILIHGYNGVELCDWLSSNSLGRLVSIELSSCRKLQHL 660
           D+     YE VL C QP SNL++I I+GY GV+LC+W+SS  LG LV++ L  C +L+HL
Sbjct: 601 DELEDVIYESVLDCLQPHSNLRKIYINGYGGVKLCNWVSSKFLGCLVTVRLYHCERLRHL 660

Query: 661 PHFDQLPYLKRLHLENLPCIEYIIDNNDFVV-----FPSLEKLSIVKMSKLKKWWRISGL 692
           P FDQ P LKRL LE LP IEYII NN+  V     FPS+++L I  M KL  W + + L
Sbjct: 661 PRFDQFPNLKRLSLEVLPNIEYIIVNNNDSVSSSTNFPSMKELEISNMPKLVSWCKGTTL 720

BLAST of PI0016321 vs. NCBI nr
Match: KAA0058598.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 561.2 bits (1445), Expect = 1.3e-155
Identity = 392/973 (40.29%), Postives = 464/973 (47.69%), Query Frame = 0

Query: 1   MAQPLLFNLIADIIFKLDSSAFRQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEKQFKN 60
           MA+ +L+N  ADIIFKL SSA ++ GSLW  V  E  KLKHSLSAIQAVLHDAEE+Q KN
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLW-RVNSELHKLKHSLSAIQAVLHDAEEQQSKN 60

Query: 61  HDVKSWEF----------------------------------QGLSMFCTRLMTWWTKFL 120
           + VK W                                    Q   +F      W     
Sbjct: 61  NQVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHK 120

Query: 121 TKSLKHKLSLEME------------------EASKSHRSS-------------------- 180
            K ++ +L    E                  E  +  R +                    
Sbjct: 121 IKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVI 180

Query: 181 ---TNSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKT--QFQLKLWVHVYDKFDLKI 240
               NSNTKEDIAIVSIVGMGGLGKT LAQ+IYNH  +    QFQ+KLWV V ++FDLKI
Sbjct: 181 DLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKI 240

Query: 241 IIQKIIESATRKRPESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLM 300
           IIQKIIESAT+K+P+SFLQIDSLQ ELRK+IDG  YLLVMDDVWNE +K+EWL+LK+LLM
Sbjct: 241 IIQKIIESATKKKPKSFLQIDSLQHELRKQIDGKKYLLVMDDVWNE-KKEEWLNLKKLLM 300

Query: 301 GGAKGSRILITTRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQ 360
           GGAKGSRILITTRSEQVAKT DST  H LQ+LD SNSWL FQKMTGLEG   NQE   DQ
Sbjct: 301 GGAKGSRILITTRSEQVAKTFDSTFVHLLQILDTSNSWLLFQKMTGLEGRLINQEAELDQ 360

Query: 361 KNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFK-KNLYSLLGQGLGIELG 420
           KNSNLI+IG EIVS LNGVPL I  IG LLKDNKSERFW SFK K L+ +L Q     L 
Sbjct: 361 KNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQD-ALK 420

Query: 421 HLLPILKLSFEHLPAI-LKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ------------- 480
            +  IL+LS+++LP+  LKQCF  YC LFPK ++I+KD LI  WRAQ             
Sbjct: 421 EMQLILELSYKYLPSSNLKQCF-RYCALFPKDYKIQKDELILLWRAQGFIQPNGNNDDDN 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 YLVDIGDDYFMQLLSRSFFQEVEKDALGEIITCKMHDLMHDLACSIANHECVRGLKGNDI 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 DKRTRHFFLEGGSRENQLMGHLSKAKYLRTFFRQDHYGVYSQWKNFKEIIFCNIFRLRAW 600

Query: 601 ------------------------------------------------------------ 628
                                                                       
Sbjct: 601 HSQLIGGIEDVESLEFIAKLKHLRYLRMKNCSVISTDLLDCIMKLYNLETFIFQSRGLKS 660

BLAST of PI0016321 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 227.3 bits (578), Expect = 4.3e-59
Identity = 186/688 (27.03%), Postives = 286/688 (41.57%), Query Frame = 0

Query: 117 IAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKIIESATRKR 176
           I +V+IVG+GG+GKT L+Q +YN + +++ F  K+W HV ++FD+  I +K+ ES T  R
Sbjct: 196 ITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVT-SR 255

Query: 177 PESFLQIDSLQRELRKEIDGNN--YLLVMDDVWNEDQKQEWLHLKRLLMGGAKGSRILIT 236
           P  F  +D LQ +L++ + G    +LLV+DD+WNE+   +W  L++  +  A+GS+IL+T
Sbjct: 256 PCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNEN-FADWDLLRQPFIHAAQGSQILVT 315

Query: 237 TRSEQVAKTCDSTSTHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFDQKNSNLIQIGKE 296
           TRS++VA    +   H LQ L   + W  F K         NQE      N  +  + + 
Sbjct: 316 TRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKTV-----FGNQEPCL---NREIGDLAER 375

Query: 297 IVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELGHLLPILKLSFEH 356
           IV    G+PLA+ T+G +L+       W    + + S     L  +  +LLP+L++S+ +
Sbjct: 376 IVHKCRGLPLAVKTLGGVLRFEGKVIEW----ERVLSSRIWDLPADKSNLLPVLRVSYYY 435

Query: 357 LPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQ-------------------------- 416
           LPA LK+CF  YC++FPKG   EKD ++  W A+                          
Sbjct: 436 LPAHLKRCFA-YCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESR 495

Query: 417 ------------------------------------------------------------ 476
                                                                       
Sbjct: 496 SLLQKTKTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFE 555

Query: 477 ------------------------------------------------------------ 536
                                                                       
Sbjct: 556 ALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKN 615

Query: 537 ------------EIEFLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGCT 596
                       E+E LP  +  ++NL+TL+L+ CS LKELP    N I L++LD+ G T
Sbjct: 616 ISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-T 675

Query: 597 SLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQEN 628
            L  MPR    L +L+T+  F +    GS ++EL GL +L G L +  LQ    VDV + 
Sbjct: 676 KLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQ--RVVDVADA 735

BLAST of PI0016321 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 183.7 bits (465), Expect = 5.4e-46
Identity = 201/832 (24.16%), Postives = 317/832 (38.10%), Query Frame = 0

Query: 12  DIIFKLDSSAF-RQYGSLWGGVEDEFDKLKHSLSAIQAVLHDAEEK--QFKNHDVKSWEF 71
           DI+ +L + A  R+  +  GG+   F  L     AIQ  +    EK  +   H VK  E 
Sbjct: 79  DILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEV 138

Query: 72  QGLSMFCTRLMTWWTKFLTKSLKHKL-------SLEMEEASKSHRSSTNSNTKEDIAIVS 131
            GL  +       W +  ++S    L        +E + A  +   S +  +    A++S
Sbjct: 139 IGLKEYSETREPQWRQ-ASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVIS 198

Query: 132 IVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDL----KIIIQKIIESATRKRP 191
           +VGM G+GKT L + ++N  R+   F++K+W+     F++    K ++Q I  SA     
Sbjct: 199 VVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTE- 258

Query: 192 ESFLQIDSLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLMGGAKGSRILITTRS 251
                + SLQ +L+K + G  +LLV+DD W+E    EW   +       +GS+I++TTRS
Sbjct: 259 ----DLPSLQIQLKKTLSGKRFLLVLDDFWSESD-SEWESFQVAFTDAEEGSKIVLTTRS 318

Query: 252 EQVAKTCDSTSTHYLQLLDASNSWLWFQKMT--GLEGCSNNQEVVFDQKNSNLIQIGKEI 311
           E V+    +   + ++L+     W    +     +   S NQE         L  IGK I
Sbjct: 319 EIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSINQE---------LEGIGKRI 378

Query: 312 VSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELGHLLPILKLSFEHL 371
                G+PLA   I   L+   +   W +  KN  S            +LP+LKLS++ L
Sbjct: 379 AEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSYTNS--------ILPVLKLSYDSL 438

Query: 372 PAILKQCFLYYCTLFPKGFQIEKDVLIRQWRA---------------------------- 431
           P  LK+CF   C++FPKG   +++ L+  W A                            
Sbjct: 439 PPQLKRCFA-LCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQS 498

Query: 432 ------------------------------------------------------------ 491
                                                                       
Sbjct: 499 FFQRLDITMTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVA 558

Query: 492 ------------------------------------------------------------ 551
                                                                       
Sbjct: 559 FRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLK 618

Query: 552 ------------QEIEFLPDCITELHNLETLILNSCSKLKELPRSTKNWIKLKHLDMHGC 611
                        +I+ LP+ +  L NL+TL+L++C  L  LP+S    I L+ LD+ G 
Sbjct: 619 GLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG- 678

Query: 612 TSLTHMPRGLCGLTNLRTMNLFVLGKDYGSGLNELNGLGNLRGSLTVKGLQFCTTVDVQE 655
           T L  MP G+  L +L+ ++ FV+G+  G+GL+EL  L +LRG+L +  LQ        +
Sbjct: 679 TPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAK 738

BLAST of PI0016321 vs. TAIR 10
Match: AT1G58390.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 135.6 bits (340), Expect = 1.7e-31
Identity = 102/338 (30.18%), Postives = 171/338 (50.59%), Query Frame = 0

Query: 114 KEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKIIESAT 173
           +E++ +VSI GMGGLGKT LA+ ++NHE +K QF    WV V  +F  K + Q I+++ T
Sbjct: 180 EENVQVVSITGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKNVWQMILQNLT 239

Query: 174 -RKRPESFLQID--SLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLMGGAKGSR 233
            R++ +  LQ++   L  +L + ++ +  L+V DD+W +   ++W  +K +     KG +
Sbjct: 240 SREKKDEILQMEEAELHDKLFQLLETSKSLIVFDDIWKD---EDWDLIKPIFPPN-KGWK 299

Query: 234 ILITTRSEQVAKTCDSTSTHYL-QLLDASNSWLWFQKMTGLEGCSNNQEVVFDQKNSNLI 293
           +L+T+++E VA   D    ++  + L   +SW  FQ++   +  ++  +V     +  + 
Sbjct: 300 VLLTSQNESVAVRGDIKYLNFKPECLAIEDSWTLFQRIAFPKKDASESKV-----DEEME 359

Query: 294 QIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELGHLLPILK 353
            +GK+++    G+PLAI  +G LL    +   W     N+ S +          +  +L 
Sbjct: 360 DMGKQMLKHCGGLPLAIKVLGGLLAAKYTMHDWERLSVNIGSDIVGRTSSNNSSIYHVLS 419

Query: 354 LSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQEIEFLPDCITELHNLETLILN 413
           +SFE LP+ LK CFLY    FP+  +I  + L   W A+ I    D     HN ET+   
Sbjct: 420 MSFEELPSYLKHCFLYLAH-FPEDHKINVEKLSYCWAAEGISTAED----YHNGETIQDV 479

Query: 414 SCSKLKELPRSTK-NWIKLKHLDMHGCTSLTHMPRGLC 447
             S L+EL R     W +       G   L  M R +C
Sbjct: 480 GQSYLEELVRRNMIIWERDATASRFGTCHLHDMMREVC 503

BLAST of PI0016321 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 131.0 bits (328), Expect = 4.2e-30
Identity = 89/280 (31.79%), Postives = 148/280 (52.86%), Query Frame = 0

Query: 110 NSNTKEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKII 169
           + N K+   I+SI GMGGLGKT LA+ +YN   +K +F  + W +V  ++  + I+ +II
Sbjct: 178 SDNEKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRII 237

Query: 170 ESATRKRPESFLQIDSLQRELRKEI------DGNNYLLVMDDVWNEDQKQEWLHLKRLLM 229
            S      E   +I   + +   E+      +G NY++V+DDVW+ D    W  LKR L 
Sbjct: 238 RSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDPD---AWESLKRALP 297

Query: 230 GGAKGSRILITTRSEQVAKTCDST-STHYLQLLDASNSWLWFQKMTGLEGCSNNQEVVFD 289
              +GS+++ITTR   +A+  + T   H L+ L    SW  F++    +  SN ++V   
Sbjct: 298 CDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFER----KAFSNIEKV--- 357

Query: 290 QKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGLGIELG 349
             + +L + GKE+V    G+PLAI+ +  LL   ++   W     +L+  L         
Sbjct: 358 --DEDLQRTGKEMVKKCGGLPLAIVVLSGLLSRKRTNE-WHEVCASLWRRLKD----NSI 417

Query: 350 HLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLI 383
           H+  +  LSF+ +   LK CFLY+ ++FP+ ++I+ + LI
Sbjct: 418 HISTVFDLSFKEMRHELKLCFLYF-SVFPEDYEIKVEKLI 439

BLAST of PI0016321 vs. TAIR 10
Match: AT1G58602.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 130.6 bits (327), Expect = 5.5e-30
Identity = 89/293 (30.38%), Postives = 156/293 (53.24%), Query Frame = 0

Query: 114 KEDIAIVSIVGMGGLGKTVLAQTIYNHERIKTQFQLKLWVHVYDKFDLKIIIQKII---- 173
           +++  +VSI GMGGLGKT LA+ ++NH+ +  +F    WV V   F LK + Q I+    
Sbjct: 179 EDNYQVVSITGMGGLGKTTLARQVFNHDMVTKKFDKLAWVSVSQDFTLKNVWQNILGDLK 238

Query: 174 --ESATRKRPESFLQID--SLQRELRKEIDGNNYLLVMDDVWNEDQKQEWLHLKRLLMGG 233
             E  T++  +  L++   +LQREL + ++ +  L+V+DD+W   +K++W  +K  +   
Sbjct: 239 PKEEETKEEEKKILEMTEYTLQRELYQLLEMSKSLIVLDDIW---KKEDWEVIKP-IFPP 298

Query: 234 AKGSRILITTRSEQVAKTCDSTSTHYL----QLLDASNSWLWFQKMTGLEGCSNNQEVVF 293
            KG ++L+T+R+E +      T+T Y     + L   +SW  FQ++      ++  E+  
Sbjct: 299 TKGWKLLLTSRNESIVA---PTNTKYFNFKPECLKTDDSWKLFQRIAFPINDASEFEI-- 358

Query: 294 DQKNSNLIQIGKEIVSTLNGVPLAIITIGILLKDNKSERFWSSFKKNLYSLLGQGL---- 353
              +  + ++G++++    G+PLAI  +G +L +  +   W    +N+ S L  G     
Sbjct: 359 ---DEEMEKLGEKMIEHCGGLPLAIKVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFN 418

Query: 354 GIELGHLLPILKLSFEHLPAILKQCFLYYCTLFPKGFQIEKDVLIRQWRAQEI 391
                    +L LSFE LP+ LK CFLY    FP+ ++I+ + L   W A+EI
Sbjct: 419 DDNNNSCNYVLSLSFEELPSYLKHCFLYLAH-FPEDYEIKVENLSYYWAAEEI 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XBQ92.0e-6927.99Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA429.3e-6728.52Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA399.3e-6727.84Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA401.6e-6627.97Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR46.0e-5827.03Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A5D3CF632.2e-18351.75Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
A0A5A7UYL53.8e-17245.84Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
A0A5D3CEX26.5e-17249.44Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
A0A5A7UTU86.5e-15640.29Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
A0A5D3CJK71.5e-15540.18Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
TYK10411.14.4e-18351.75putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. m... [more]
XP_031736581.14.2e-17342.64putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothe... [more]
KAA0058609.17.9e-17245.84putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. m... [more]
TYK10413.11.3e-17149.44putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. m... [more]
KAA0058598.11.3e-15540.29putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
AT3G14470.14.3e-5927.03NB-ARC domain-containing disease resistance protein [more]
AT3G14460.15.4e-4624.16LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G58390.11.7e-3130.18Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G46730.14.2e-3031.79NB-ARC domain-containing disease resistance protein [more]
AT1G58602.15.5e-3030.38LRR and NB-ARC domains-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 193..207
score: 32.83
coord: 398..414
score: 34.85
coord: 119..134
score: 53.12
coord: 299..313
score: 36.17
NoneNo IPR availableGENE3D1.20.5.4130coord: 10..75
e-value: 3.3E-8
score: 35.5
NoneNo IPR availablePANTHERPTHR23155:SF1055OS05G0414200 PROTEINcoord: 37..389
coord: 390..656
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 390..690
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 85..251
e-value: 1.2E-35
score: 124.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 114..365
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 114..353
e-value: 4.1E-39
score: 134.3
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 16..66
e-value: 3.3E-11
score: 43.3
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 367..717
e-value: 1.4E-26
score: 94.7
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 255..355
e-value: 5.8E-8
score: 34.2
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 37..389
coord: 390..656

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016321.1PI0016321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding