PI0016094 (gene) Melon (PI 482460) v1

Overview
NamePI0016094
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAlpha-galactosidase
Locationchr10: 17070491 .. 17074758 (+)
RNA-Seq ExpressionPI0016094
SyntenyPI0016094
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAAACCTCACTTCCTCCATAAATTTCTTGTAGTTCAGTGAAGAAGCATTGCTAAATCCTGTCTCTCAATGCTTCTGCTCTGAGGTAACGAATCAACCTGTTCTGATCTAAGACTAACTCTGTAAATGGTACATTCACCATTAATACTCAATCACAGGCTACAACCATGGCTTTCTCCCTACTCTCGCTTTCATCTTCACCTTCAGTTATGGTTCTACTCTACTCCATCCTTTTCTGGACGTTCTTGCTAGGTAGCAATGGCAATGGCAATGGCGTTGCGGTGGCCGGAGCTTCTCGATCGACAGTTCTAAAGTTTGCTGCTGAGTTTGATTCTGTTTCCTCTAGGAGAGTTCTTCTCAACAATGGTCTCGCTCTAACTCCTCCGATGGGGTAAGTATTATGCTCCGATCATGATTTTTAAGCAGAATTGATTCGATTGAATCAAATGTTGTTTGTTTTTTTTTATTGTGATCTTCTTGGTCTTGTTCAATAGTGTTTATTGATAATTTGATGGTGGTGGATTGCTGATTTTTGTAATGTGTTGCTTTGCTTCCGGCTTTTGTAATCAACCCAGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGGTGATGATTTTGATTTGAAGGCGTTTTTCTGCTTGGATTTGTGTACTGTGAGTTTGAGACTGAGATACACATACTATTTCTTTTGCTAAGCGGATGCAATGGTGACCAGCGGCCTTGCTGCATTAGGATATGAGTATATCAATTTAGGTGATGATTTTATTTCTTTTTTGTACATTTCTTTGGTTTTTCAACTTCATGCTTTCTGTTTTTGACATTAATATGATCATGTTTCCATATGAGTTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGTAAATTTGAATGTCCTATTTCCTGCCTAGGATTTCTATGAAAATCTCTTTAATTGCATTAAATGATTTCCATCAAAATTTTCAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATCATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGTAAGTTTATACACTCATCAGATCAGCTTAAAAAACTTCCTGATCTTCCATTTCAATTGTTGCAATTCATTCAAATGCTGCAGCTGTTTTTTCTTTTTTTTCTGGCTGAAAGAAATTTCATTATCAACAGATTCAACACTGTTTTAGTTCAATCTTCTTTACTTAAGTTATATAATTTCTTCAGGATCCAAACTTGCAGTAAAAAGATGCCCGGTTCCCTTGGTCATGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGTATTTTTTGCTGCTCTAGTCTACACTCTTAAAGCTGTTCTTGTCCCTTGCCCTTAGGCTGTTCTCTTTTTGTTCTTTGAATATATCTATTTCTTATAAAAAAAAAAAAAATAGTCTACACTCTTAAAGCTTAACTCATTTAACTCTTGTTTATAATGAATGATTTTTTGCTTAGAAACTGATATATACACAATTGTACAGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTAGGATTAGCTTTCTACGTTCCAGTATCAAAATTTCCAAATGACTGCAACAATTCCAGGGGTACAGGTATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGTTAGGAATCTTTTATACACAGAAAAAAATGCTATAGTTGTTCTAGTGCTTCTCCATTCTAAAGCACAGACCGAGTTCCTCTCTTAAAATTTTCCTTATGTAGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGGTAGTGACTAGTGAGCATACATTCGATCATTTCTCGGAACACTCAGTTTTTTGAAAGACTGTCTACAGTCCCTTTTGTCTATCTATGAATATGGCTGTACTGAAGTACGATCTTTCAAGTCCAAAATACTTTCTTTAGCAAGAAACTAATGCTCCTACTTAATGATTACAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGGTAAATTCATAATTGTAGCTCAATATGATAATTAAACACGTAATCTTGTGTATAGAGAAACATTGGCTGGTTAGCTTGTTGGTACTTAGTAAAGATTCAAAGGATTAAATCCAATCGAGTGCTGTATCTGAACTGTTTAAACTCATCAACCTTAAATAACCTTGTTTGAGGAAAATTATTTGAAAGGAATAGTTAAATATGACAGTCAGAATATTCATATGTATTAACTTATTGTCAAACAATTCCTTTTTATGATTATGACTAAATAACCTTCTCTATGTCCTCAAAATCCTTTCCCTTTGGCTAACCTCTTTAGAAGAATACAACAATCTCAGAAATGCTCAGGAAAATAATCTTAGTTATTTCTTTTGTTAAGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACCGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGTAAGTACAATTGAGTAGAAATTTTCAGTTCTAGTTTAGATGGACGACCTTAGGATATGTTCTATAGGTTGTACAACTGTCTCCTTGAGTTTAGATAGGGAGTTTCTTGTACCATATCAGGACATATTCGGAATTCAAGTTCATTAAATAACAAGATAGGGTTGAACATCTTATGTTCCTCTATTCGTGGGTTGAGTGTATAATCTTGTGTGCTTCGTAAAATATCCAAATCATGTTATAATCGATTCCTTTCTGTTCTTCATATATGAAGGCCCCATTGCTGATTGGATGTGACATCAGATCAATGGATAACATCACCATGAAATTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGGTAAGTAAAAACCACACTGTTTCTGCTAATTCATAGAGAACTGTGAAACAGATAAACATCTAATAATTTCTCCTAATAAAATGGAAAGATTAATTGTTGAAGAATACTTAGATGCATCTTAGATTGCACCCATCTGTTTACAGTCGAGGATACACTCAAGCATTATTCTTGTTCGTTAACTAAATATGACACTATGTGCGGCCATCTAAAAGTTTTTTATCCAAGTAGATAAGCTTGGAGTACAAGGAAAGAAGGTCTATAAATATGGAGACCTCGAGGTAATTTTTCATCATCTAACGTATTCCCTAAGGAAATTAAAAGAGAGAAAAAAAGGGTAAATCTCTACTTTATTCTTTAACTTTTCTACACAAAACCAATGCAGGTTTGGGCAGGACCGCTGAGTGGTAAAAGAGTCGCCGTGGTTCTATGGAACAGAGGTTTATGGCAAGCCAATATCACTGCATCTTGGTCTGATATAGGTTTAAGCTCATCAACTACAGTTACTGCTCGAAACTTATGGGAGGTAAGATATGAATCATCTTGTAACTAAATCCATTTTTCAAAGTTTTTTTTGGCATTAGGTTACCTAGTTAAAAACCACATTTTAGTATATCATATTATTATAGGTAGTGATTTAGCTCATATACTTTTAAATTTATAAAACTTTAATCACTTAATCTTAGTATGTAACGATTTAGTCTCTATATTCTTGATTTTGTATTGATTTAGTCTATGTACTCTAAATGTTGTATTGGGTTAATCCTTATCATAAGAATTATTATTGAAATAATGATATTTATATTACGTATGCATATTGATAAATTTATTCGGAACCAAATGTGATTATAAACTACAAAAACTAAATTACTATGAAAATTGTTTTTTAAGTAAGCACATAATTAATATAGTACAGTTTTTATAAACAAATGTGAACTCTGTGTCAAAATTGAATTGATCCTATTTTATATGAGTGTATATATTTGTTCATCATTCATAAGACCTGTTCTTTTTTTCCTTCTGTCTTTTGACTAATTATTCATTCCTAACAACAGCATTCCAGCCATCTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTAATTAGCCAAATGAGATAGCTTTCTTTCTGTATGATTATTGTCTTCACAATTCATCTACATGTCCTTCCACTCTATGTGCTTGTGTTTTCTTGTATTTTACAATACACATGAAATATATATTTTGCTTCCTCCCA

mRNA sequence

GACAAACCTCACTTCCTCCATAAATTTCTTGTAGTTCAGTGAAGAAGCATTGCTAAATCCTGTCTCTCAATGCTTCTGCTCTGAGGCTACAACCATGGCTTTCTCCCTACTCTCGCTTTCATCTTCACCTTCAGTTATGGTTCTACTCTACTCCATCCTTTTCTGGACGTTCTTGCTAGGTAGCAATGGCAATGGCAATGGCGTTGCGGTGGCCGGAGCTTCTCGATCGACAGTTCTAAAGTTTGCTGCTGAGTTTGATTCTGTTTCCTCTAGGAGAGTTCTTCTCAACAATGGTCTCGCTCTAACTCCTCCGATGGGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTGACCAGCGGCCTTGCTGCATTAGGATATGAGTATATCAATTTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATCATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGATCCAAACTTGCAGTAAAAAGATGCCCGGTTCCCTTGGTCATGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACCGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCCCCATTGCTGATTGGATGTGACATCAGATCAATGGATAACATCACCATGAAATTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTACAAGGAAAGAAGGTCTATAAATATGGAGACCTCGAGGTTTGGGCAGGACCGCTGAGTGGTAAAAGAGTCGCCGTGGTTCTATGGAACAGAGGTTTATGGCAAGCCAATATCACTGCATCTTGGTCTGATATAGGTTTAAGCTCATCAACTACAGTTACTGCTCGAAACTTATGGGAGCATTCCAGCCATCTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTAATTAGCCAAATGAGATAGCTTTCTTTCTGTATGATTATTGTCTTCACAATTCATCTACATGTCCTTCCACTCTATGTGCTTGTGTTTTCTTGTATTTTACAATACACATGAAATATATATTTTGCTTCCTCCCA

Coding sequence (CDS)

ATGGCTTTCTCCCTACTCTCGCTTTCATCTTCACCTTCAGTTATGGTTCTACTCTACTCCATCCTTTTCTGGACGTTCTTGCTAGGTAGCAATGGCAATGGCAATGGCGTTGCGGTGGCCGGAGCTTCTCGATCGACAGTTCTAAAGTTTGCTGCTGAGTTTGATTCTGTTTCCTCTAGGAGAGTTCTTCTCAACAATGGTCTCGCTCTAACTCCTCCGATGGGATGGAATAGTTGGAACCATTTTCAATGTAATTTAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTGACCAGCGGCCTTGCTGCATTAGGATATGAGTATATCAATTTAGATGATTGTTGGGCTGAACTTGACAGAGACTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATCATGTTCATAGAAAAGGGCTCAAGCTTGGAATTTATTCGGATGCTGGGATCCAAACTTGCAGTAAAAAGATGCCCGGTTCCCTTGGTCATGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATTGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTATCCAAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACAACATCAGATATTCAAGATAACTGGATTAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCTTCTTACGCTAAACCAGGAGGATGGAATGATCCCGACATGCTTGAAGTGGGGAATGGAGGGATGACCACCGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCCCCATTGCTGATTGGATGTGACATCAGATCAATGGATAACATCACCATGAAATTGTTAAGTAACAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTACAAGGAAAGAAGGTCTATAAATATGGAGACCTCGAGGTTTGGGCAGGACCGCTGAGTGGTAAAAGAGTCGCCGTGGTTCTATGGAACAGAGGTTTATGGCAAGCCAATATCACTGCATCTTGGTCTGATATAGGTTTAAGCTCATCAACTACAGTTACTGCTCGAAACTTATGGGAGCATTCCAGCCATCTGGTTCAACATCACCTTACTGCTCAAGTAGACTCTCATGACTGTAAGATGTTTGTTCTCACACCTCACTAA

Protein sequence

MAFSLLSLSSSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH
Homology
BLAST of PI0016094 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.6e-177
Identity = 292/379 (77.04%), Postives = 330/379 (87.07%), Query Frame = 0

Query: 46  TVLKFAAEFDSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAA 105
           T+ + A  F S    R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMV+SGL+A
Sbjct: 18  TLTQIADGFQS----RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSA 77

Query: 106 LGYEYINLDDCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCS 165
           +GY+YIN+DDCW EL RDS+G+LVAKASTFPSGIKAL+D+VH KGLKLGIYSDAG  TCS
Sbjct: 78  IGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCS 137

Query: 166 KKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLC 225
           + MPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLC
Sbjct: 138 QTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLC 197

Query: 226 EWGQEDPATWAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGN 285
           EWGQEDPATWA ++GNSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGN
Sbjct: 198 EWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGN 257

Query: 286 GGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVY 345
           GGMT  EY SHFSIWALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV 
Sbjct: 258 GGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK 317

Query: 346 KYGDLEVWAGPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSH-LV 405
           K GDLEVWAGPLS KRVAV+LWNRG   ANITA W++IGL+SS  V AR+LWEHS++  V
Sbjct: 318 KEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV 377

Query: 406 QHHLTAQVDSHDCKMFVLT 424
           +  L+A V+ H CKM+ LT
Sbjct: 378 KKQLSALVEPHACKMYTLT 392

BLAST of PI0016094 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 9.8e-175
Identity = 290/367 (79.02%), Postives = 314/367 (85.56%), Query Frame = 0

Query: 59  SRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWA 118
           +RR LL NGL LTPPMGWNSWNHF+CNL+E LI+ETADAMV+ GLAALGY+YINLDDCWA
Sbjct: 11  TRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWA 70

Query: 119 ELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQD 178
           EL+RDS+GNLV K STFPSGIKALAD+VH KGLKLGIYSDAG QTCSK MPGSLGHEEQD
Sbjct: 71  ELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQD 130

Query: 179 AKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVN 238
           AKTFASWG+DYLKYDNC N   SPKERYP M+KAL  SGR I FSLCEWG+EDPATWA  
Sbjct: 131 AKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKE 190

Query: 239 VGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFS 298
           VGNSWRTT DI D+W SMT+ AD NDKWASYA PGGWNDPDMLEVGNGGMTT EYRSHFS
Sbjct: 191 VGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFS 250

Query: 299 IWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLS 358
           IWALAKAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG KV  YGDLEVWAGPLS
Sbjct: 251 IWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLS 310

Query: 359 GKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSH-LVQHHLTAQVDSHDC 418
           GKRVAV LWNRG   A ITA WSD+GL S+  V AR+LW HS+   V+  ++A VD+HD 
Sbjct: 311 GKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAHDS 370

Query: 419 KMFVLTP 425
           KM+VLTP
Sbjct: 371 KMYVLTP 377

BLAST of PI0016094 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 4.1e-173
Identity = 280/375 (74.67%), Postives = 320/375 (85.33%), Query Frame = 0

Query: 51  AAEFDSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEY 110
           +AE +  + RR L  NGL  TPPMGWNSWNHF C++NEN+++ETADAMV++GLAALGY+Y
Sbjct: 35  SAEAEHYNVRRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGYQY 94

Query: 111 INLDDCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPG 170
           INLDDCWAEL+RDS+GN+V  A+ FPSGIKALAD+VH KGLKLG+YSDAG QTCSK+MPG
Sbjct: 95  INLDDCWAELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRMPG 154

Query: 171 SLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQE 230
           SLGHEEQDAKTFASWG+DYLKYDNCEN G S KERYP M KAL  SGRPI FS+CEWG E
Sbjct: 155 SLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWGWE 214

Query: 231 DPATWAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT 290
           DP  WA ++GNSWRTT DI+DNW SMT+IAD NDKWASYA PGGWNDPDMLEVGNGGMTT
Sbjct: 215 DPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGGMTT 274

Query: 291 AEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDL 350
            EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+SN EVIAVNQDKLGVQGKKV    DL
Sbjct: 275 EEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTNDL 334

Query: 351 EVWAGPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSH-LVQHHLT 410
           EVWAGPLS  +VAV+LWNR   +A +TASWSDIGL   TTV AR+LWEHS+  LV   ++
Sbjct: 335 EVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHSTQSLVSGEIS 394

Query: 411 AQVDSHDCKMFVLTP 425
           A++DSH CKM+VLTP
Sbjct: 395 AEIDSHACKMYVLTP 409

BLAST of PI0016094 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 2.9e-158
Identity = 268/415 (64.58%), Postives = 312/415 (75.18%), Query Frame = 0

Query: 11  SPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLAL 70
           SP +++LL   +  T L  +   GN  A +  +R         + S  +RR    NGL  
Sbjct: 12  SPRLLLLLLVAVAATLLPEAAALGNFTAESRGAR---------WRSRRARRRAFENGLGR 71

Query: 71  TPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLVA 130
           TP MGWNSWNHF C +NE +I+ETADA+V +GLA LGY+Y+N+DDCWAE  RDS+GN V 
Sbjct: 72  TPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDSQGNFVP 131

Query: 131 KASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYL 190
              TFPSGIKALAD+VH KGLKLGIYSDAG QTCS KMPGSL HEEQD KTFASWG+DYL
Sbjct: 132 NRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDVKTFASWGVDYL 191

Query: 191 KYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQ 250
           KYDNC + G S  ERY +M+ A++  G+ I FSLCEWG+E+PATWA  +GNSWRTT DI 
Sbjct: 192 KYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWRTTGDIA 251

Query: 251 DNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIG 310
           DNW SMT+ AD+ND+WA+YA PGGWNDPDMLEVGNGGM+ AEYRSHFSIWALAKAPLLIG
Sbjct: 252 DNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIG 311

Query: 311 CDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNRG 370
           CD+RSM   T  +LSN EVIAVNQD LGVQGKKV     LEVWAGPLS  R AVVLWNR 
Sbjct: 312 CDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVLWNRQ 371

Query: 371 LWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
            +QA ITA WS+IGL+ S  VTAR+LW HSS   Q  ++A V  HDCKM+VLTP+
Sbjct: 372 SYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQISASVAPHDCKMYVLTPN 417

BLAST of PI0016094 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 4.4e-151
Identity = 249/372 (66.94%), Postives = 297/372 (79.84%), Query Frame = 0

Query: 55  DSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLD 114
           DS   RR LL NGL +TPPMGWNSWNHF CN++E +IKETADA+VT+GL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 174
           DCWAE+ RDSKG+LV K STFPSGIKA+AD+VH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 175 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 234
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 235 WAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 294
           W   VGNSWRTT+DI+D W+SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 295 SHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWA 354
            HFSIWA++KAPLL+GCDIR+M   TM++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 355 GPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSS--HLVQHHLTAQV 414
           GPLSG RVA++L NRG  + +ITA W DI + +++ V AR+LWEH +       +LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 415 DSHDCKMFVLTP 425
           DSH CK++VL P
Sbjct: 397 DSHACKLYVLKP 408

BLAST of PI0016094 vs. ExPASy TrEMBL
Match: A0A1S3BEH7 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 6.5e-238
Identity = 400/416 (96.15%), Postives = 409/416 (98.32%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVMVLLY +L WTFLLG  GNGNGVAVAGASRST L+FAAEFDSVSSRRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV+SGLAALGYEYINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 369
           GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR
Sbjct: 306 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 365

Query: 370 GLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           GLW+ANITASWSDIGL SSTTVTAR+LW+HSS +VQHHLTAQVDSHDCKMFVLTPH
Sbjct: 366 GLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 419

BLAST of PI0016094 vs. ExPASy TrEMBL
Match: A0A5A7SVZ2 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003280 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.6e-236
Identity = 400/417 (95.92%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVMVLLY +L WTFLLG  GNGNGVAVAGASRST L+FAAEFDSVSSRRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV+SGLAALGYEYINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN 369
           GCDIRSMDNITMKLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN
Sbjct: 306 GCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN 365

Query: 370 RGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           RGLW+ANITASWSDIGL SSTTVTAR+LW+HSS +VQHHLTAQVDSHDCKMFVLTPH
Sbjct: 366 RGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 420

BLAST of PI0016094 vs. ExPASy TrEMBL
Match: Q2HYY3 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.6e-233
Identity = 388/416 (93.27%), Postives = 403/416 (96.88%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVM+LLY +LFWTFLLG NGNGNGV VA ASRST L+FA EFDS SSRR+LLNNGLA
Sbjct: 6   SSPSVMLLLYFLLFWTFLLGGNGNGNGVVVAAASRSTALRFATEFDSASSRRILLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV++GLAALGY+YINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGI+TCSK+MPGSLGHEEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMT AEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 369
           GCDIRSMDN TMKLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAG LSGKRVAVVLWNR
Sbjct: 306 GCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRVAVVLWNR 365

Query: 370 GLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
            LW+ANITA WSDIGLSSSTTVTAR+LWEHSS +V+HHLTAQVDSHDCKMFVLTPH
Sbjct: 366 SLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVLTPH 421

BLAST of PI0016094 vs. ExPASy TrEMBL
Match: A0A6J1FZL3 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.4e-221
Identity = 372/418 (89.00%), Postives = 393/418 (94.02%), Query Frame = 0

Query: 8   LSSSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNG 67
           +SS  S+M+ LYS+LFWTFL  SNGNG   A+AGA+RS  L+FAAEFD  S RRVLLNNG
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSSNGNGG--AMAGATRSRPLRFAAEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMV+SGLAALGY+YINLDDCWAEL+RD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNIT+KLLSNK VIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLW 361

Query: 368 NRGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           NRG ++A ITASWS IGLS STTVTAR+LWEHSS +VQH +TAQVD HDCKM+VLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

BLAST of PI0016094 vs. ExPASy TrEMBL
Match: A0A6J1HTR9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.9e-221
Identity = 372/418 (89.00%), Postives = 392/418 (93.78%), Query Frame = 0

Query: 8   LSSSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNG 67
           +SS  S+M+LLYS+LFWTF   SNGNG   A+AGA+RS  L+FA EFD  S RRVLLNNG
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSSNGNGG--AMAGATRSRPLRFATEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMV+SGLAALGY+YINLDDCWAEL+RD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSL HEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNIT+KLLSNKEVIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLW 361

Query: 368 NRGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           NRG ++A ITASWS IGLS STTVTAR+LWEHSS +VQH LTAQVD HDCKM+VLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYVLTPH 417

BLAST of PI0016094 vs. NCBI nr
Match: XP_008445911.1 (PREDICTED: alpha-galactosidase [Cucumis melo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.3e-237
Identity = 400/416 (96.15%), Postives = 409/416 (98.32%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVMVLLY +L WTFLLG  GNGNGVAVAGASRST L+FAAEFDSVSSRRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV+SGLAALGYEYINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 369
           GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR
Sbjct: 306 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 365

Query: 370 GLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           GLW+ANITASWSDIGL SSTTVTAR+LW+HSS +VQHHLTAQVDSHDCKMFVLTPH
Sbjct: 366 GLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 419

BLAST of PI0016094 vs. NCBI nr
Match: KAA0034096.1 (alpha-galactosidase [Cucumis melo var. makuwa] >TYK15823.1 alpha-galactosidase [Cucumis melo var. makuwa])

HSP 1 Score: 828.2 bits (2138), Expect = 3.3e-236
Identity = 400/417 (95.92%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVMVLLY +L WTFLLG  GNGNGVAVAGASRST L+FAAEFDSVSSRRVLLNNGLA
Sbjct: 6   SSPSVMVLLYFVLSWTFLLG--GNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV+SGLAALGYEYINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN 369
           GCDIRSMDNITMKLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN
Sbjct: 306 GCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWN 365

Query: 370 RGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
           RGLW+ANITASWSDIGL SSTTVTAR+LW+HSS +VQHHLTAQVDSHDCKMFVLTPH
Sbjct: 366 RGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTPH 420

BLAST of PI0016094 vs. NCBI nr
Match: NP_001267530.1 (alpha-galactosidase-like precursor [Cucumis sativus] >ABC88435.1 acid alpha galactosidase 2 [Cucumis sativus] >KGN51578.1 hypothetical protein Csa_008622 [Cucumis sativus])

HSP 1 Score: 818.1 bits (2112), Expect = 3.4e-233
Identity = 388/416 (93.27%), Postives = 403/416 (96.88%), Query Frame = 0

Query: 10  SSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSRRVLLNNGLA 69
           SSPSVM+LLY +LFWTFLLG NGNGNGV VA ASRST L+FA EFDS SSRR+LLNNGLA
Sbjct: 6   SSPSVMLLLYFLLFWTFLLGGNGNGNGVVVAAASRSTALRFATEFDSASSRRILLNNGLA 65

Query: 70  LTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNLV 129
           LTPPMGWNSWNHFQCNLNENLIKETADAMV++GLAALGY+YINLDDCWAELDRDSKGNLV
Sbjct: 66  LTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCWAELDRDSKGNLV 125

Query: 130 AKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 189
           AKASTFPSGIKALAD+VHRKGLKLGIYSDAGI+TCSK+MPGSLGHEEQDAKTFASWGIDY
Sbjct: 126 AKASTFPSGIKALADYVHRKGLKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTFASWGIDY 185

Query: 190 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 249
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 186 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 245

Query: 250 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 309
           QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMT AEYRSHFSIWALAKAPLLI
Sbjct: 246 QDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWALAKAPLLI 305

Query: 310 GCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVLWNR 369
           GCDIRSMDN TMKLLSNKEVIAVNQDKLGVQGKKV+KYGDLEVWAG LSGKRVAVVLWNR
Sbjct: 306 GCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRVAVVLWNR 365

Query: 370 GLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMFVLTPH 426
            LW+ANITA WSDIGLSSSTTVTAR+LWEHSS +V+HHLTAQVDSHDCKMFVLTPH
Sbjct: 366 SLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVLTPH 421

BLAST of PI0016094 vs. NCBI nr
Match: XP_038892679.1 (alpha-galactosidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 800.0 bits (2065), Expect = 9.6e-228
Identity = 384/425 (90.35%), Postives = 403/425 (94.82%), Query Frame = 0

Query: 1   MAFSLLSLSSSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSR 60
           MA     LSS PS + LL S+LFW FLLGSNG     AVAGA RS  L+FAA FDSVS+R
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGSNGG----AVAGAIRSRSLRFAAGFDSVSTR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAEL 120
           RVLLNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMV+SGLA+LGYEYINLDDCWAEL
Sbjct: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWAEL 120

Query: 121 DRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
           +RDSKGNLVAKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLGHE+QDA+
Sbjct: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQDAE 180

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+NVG
Sbjct: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAINVG 240

Query: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIW
Sbjct: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIW 300

Query: 301 ALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGK 360
           ALAKAPLLIGCDIRSMDNI +KLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGK
Sbjct: 301 ALAKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGK 360

Query: 361 RVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKMF 420
           RVAVVLWNRGLW+ANITASWSDIGLS+STTVTAR+LWEHSS +VQHHLTAQVDSHDCKM+
Sbjct: 361 RVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMY 420

Query: 421 VLTPH 426
           VLTPH
Sbjct: 421 VLTPH 421

BLAST of PI0016094 vs. NCBI nr
Match: XP_038892678.1 (alpha-galactosidase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 384/426 (90.14%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MAFSLLSLSSSPSVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSR 60
           MA     LSS PS + LL S+LFW FLLGSNG     AVAGA RS  L+FAA FDSVS+R
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGSNGG----AVAGAIRSRSLRFAAGFDSVSTR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAEL 120
           RVLLNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMV+SGLA+LGYEYINLDDCWAEL
Sbjct: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWAEL 120

Query: 121 DRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
           +RDSKGNLVAKASTFPSGIKALAD+VHRKGLKLGIYSDAGIQTCSKKMPGSLGHE+QDA+
Sbjct: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQDAE 180

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+NVG
Sbjct: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAINVG 240

Query: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIW
Sbjct: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIW 300

Query: 301 ALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQ-DKLGVQGKKVYKYGDLEVWAGPLSG 360
           ALAKAPLLIGCDIRSMDNI +KLLSNKEVIAVNQ DKLGVQGKKVYKYGDLEVWAGPLSG
Sbjct: 301 ALAKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSG 360

Query: 361 KRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSHLVQHHLTAQVDSHDCKM 420
           KRVAVVLWNRGLW+ANITASWSDIGLS+STTVTAR+LWEHSS +VQHHLTAQVDSHDCKM
Sbjct: 361 KRVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKM 420

Query: 421 FVLTPH 426
           +VLTPH
Sbjct: 421 YVLTPH 422

BLAST of PI0016094 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 622.5 bits (1604), Expect = 2.6e-178
Identity = 292/379 (77.04%), Postives = 330/379 (87.07%), Query Frame = 0

Query: 46  TVLKFAAEFDSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAA 105
           T+ + A  F S    R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMV+SGL+A
Sbjct: 18  TLTQIADGFQS----RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSA 77

Query: 106 LGYEYINLDDCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCS 165
           +GY+YIN+DDCW EL RDS+G+LVAKASTFPSGIKAL+D+VH KGLKLGIYSDAG  TCS
Sbjct: 78  IGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCS 137

Query: 166 KKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLC 225
           + MPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLC
Sbjct: 138 QTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLC 197

Query: 226 EWGQEDPATWAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGN 285
           EWGQEDPATWA ++GNSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGN
Sbjct: 198 EWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGN 257

Query: 286 GGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVY 345
           GGMT  EY SHFSIWALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV 
Sbjct: 258 GGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK 317

Query: 346 KYGDLEVWAGPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSH-LV 405
           K GDLEVWAGPLS KRVAV+LWNRG   ANITA W++IGL+SS  V AR+LWEHS++  V
Sbjct: 318 KEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV 377

Query: 406 QHHLTAQVDSHDCKMFVLT 424
           +  L+A V+ H CKM+ LT
Sbjct: 378 KKQLSALVEPHACKMYTLT 392

BLAST of PI0016094 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 621.7 bits (1602), Expect = 4.4e-178
Identity = 288/364 (79.12%), Postives = 324/364 (89.01%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAEL 120
           R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMV+SGL+A+GY+YIN+DDCW EL
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 121 DRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
            RDS+G+LVAKASTFPSGIKAL+D+VH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 301 ALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGK 360
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

Query: 361 RVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSSH-LVQHHLTAQVDSHDCKM 420
           RVAV+LWNRG   ANITA W++IGL+SS  V AR+LWEHS++  V+  L+A V+ H CKM
Sbjct: 303 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 362

Query: 421 FVLT 424
           + LT
Sbjct: 363 YTLT 366

BLAST of PI0016094 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 535.8 bits (1379), Expect = 3.1e-152
Identity = 249/372 (66.94%), Postives = 297/372 (79.84%), Query Frame = 0

Query: 55  DSVSSRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLD 114
           DS   RR LL NGL +TPPMGWNSWNHF CN++E +IKETADA+VT+GL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DCWAELDRDSKGNLVAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 174
           DCWAE+ RDSKG+LV K STFPSGIKA+AD+VH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 175 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 234
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 235 WAVNVGNSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 294
           W   VGNSWRTT+DI+D W+SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 295 SHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWA 354
            HFSIWA++KAPLL+GCDIR+M   TM++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 355 GPLSGKRVAVVLWNRGLWQANITASWSDIGLSSSTTVTARNLWEHSS--HLVQHHLTAQV 414
           GPLSG RVA++L NRG  + +ITA W DI + +++ V AR+LWEH +       +LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 415 DSHDCKMFVLTP 425
           DSH CK++VL P
Sbjct: 397 DSHACKLYVLKP 408

BLAST of PI0016094 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 511.5 bits (1316), Expect = 6.4e-145
Identity = 252/421 (59.86%), Postives = 302/421 (71.73%), Query Frame = 0

Query: 13  SVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSR----RVLLNNGL 72
           SV+ L+  +   + L+  +  G   A    S +  L F+  F+S+       R+ LNNGL
Sbjct: 10  SVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNGL 69

Query: 73  ALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNL 132
           A TP MGWNSWN F CN+NE +IKETADA+V+SGLA LGY ++N+DDCW+ L RDS+G L
Sbjct: 70  ARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRDSEGQL 129

Query: 133 VAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGID 192
           V    TFPSGIK LAD+VH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG+D
Sbjct: 130 VPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVD 189

Query: 193 YLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSD 252
           YLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG +DPA WA  VGNSWRTT D
Sbjct: 190 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 249

Query: 253 IQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLL 312
           I D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR HFSIWAL KAPLL
Sbjct: 250 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 309

Query: 313 IGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGD---LEVWAGPLSGKRVAVV 372
           IGCD+R+M   T+++LSNKE+IAVNQD LGVQG+K+   G+    +VW+GPLSG R+ V 
Sbjct: 310 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 369

Query: 373 LWNRGLWQANITASWSDIGLSSSTTVTARNLWEHS--SHLVQHHLTAQVDSHDCKMFVLT 425
           LWNR    A ITASW  IGL S+ +V+ R+LW+H   +        AQVD+HDC M+VLT
Sbjct: 370 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 429

BLAST of PI0016094 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 6.8e-131
Identity = 237/421 (56.29%), Postives = 285/421 (67.70%), Query Frame = 0

Query: 13  SVMVLLYSILFWTFLLGSNGNGNGVAVAGASRSTVLKFAAEFDSVSSR----RVLLNNGL 72
           SV+ L+  +   + L+  +  G   A    S +  L F+  F+S+       R+ LNNGL
Sbjct: 10  SVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNGL 69

Query: 73  ALTPPMGWNSWNHFQCNLNENLIKETADAMVTSGLAALGYEYINLDDCWAELDRDSKGNL 132
           A TP MGWNSWN F CN+NE +IKETADA+V+SGLA LGY ++N+               
Sbjct: 70  ARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI--------------- 129

Query: 133 VAKASTFPSGIKALADHVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGID 192
                    GIK LAD+VH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG+D
Sbjct: 130 ---------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVD 189

Query: 193 YLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSD 252
           YLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG +DPA WA  VGNSWRTT D
Sbjct: 190 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 249

Query: 253 IQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLL 312
           I D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR HFSIWAL KAPLL
Sbjct: 250 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 309

Query: 313 IGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGD---LEVWAGPLSGKRVAVV 372
           IGCD+R+M   T+++LSNKE+IAVNQD LGVQG+K+   G+    +VW+GPLSG R+ V 
Sbjct: 310 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 369

Query: 373 LWNRGLWQANITASWSDIGLSSSTTVTARNLWEHS--SHLVQHHLTAQVDSHDCKMFVLT 425
           LWNR    A ITASW  IGL S+ +V+ R+LW+H   +        AQVD+HDC M+VLT
Sbjct: 370 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RX863.6e-17777.04Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
Q426569.8e-17579.02Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
P147494.1e-17374.67Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q9FXT42.9e-15864.58Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Q9FT974.4e-15166.94Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BEH76.5e-23896.15Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1[more]
A0A5A7SVZ21.6e-23695.92Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0... [more]
Q2HYY31.6e-23393.27Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1[more]
A0A6J1FZL31.4e-22189.00Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1[more]
A0A6J1HTR91.9e-22189.00Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008445911.11.3e-23796.15PREDICTED: alpha-galactosidase [Cucumis melo][more]
KAA0034096.13.3e-23695.92alpha-galactosidase [Cucumis melo var. makuwa] >TYK15823.1 alpha-galactosidase [... [more]
NP_001267530.13.4e-23393.27alpha-galactosidase-like precursor [Cucumis sativus] >ABC88435.1 acid alpha gala... [more]
XP_038892679.19.6e-22890.35alpha-galactosidase-like isoform X2 [Benincasa hispida][more]
XP_038892678.12.4e-22690.14alpha-galactosidase-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G08370.12.6e-17877.04alpha-galactosidase 2 [more]
AT5G08370.24.4e-17879.12alpha-galactosidase 2 [more]
AT5G08380.13.1e-15266.94alpha-galactosidase 1 [more]
AT3G56310.16.4e-14559.86Melibiase family protein [more]
AT3G56310.26.8e-13156.29Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 102..117
score: 69.06
coord: 291..312
score: 55.91
coord: 178..195
score: 76.39
coord: 205..223
score: 52.37
coord: 270..289
score: 69.5
coord: 66..85
score: 66.5
coord: 143..164
score: 61.59
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 71..335
e-value: 3.3E-80
score: 269.3
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 50..424
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 72..335
e-value: 9.95548E-160
score: 449.313
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 64..337
e-value: 7.2E-138
score: 460.7
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 348..422
e-value: 9.9E-19
score: 67.2
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 338..425
e-value: 4.1E-29
score: 102.4
NoneNo IPR availablePANTHERPTHR11452:SF33ALPHA-GALACTOSIDASE 2coord: 50..424
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 330..423
IPR000111Glycoside hydrolase family 27/36, conserved sitePROSITEPS00512ALPHA_GALACTOSIDASEcoord: 107..123
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 62..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016094.1PI0016094.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009911 positive regulation of flower development
biological_process GO:0009620 response to fungus
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds