PI0016064 (gene) Melon (PI 482460) v1

Overview
NamePI0016064
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr08: 18497385 .. 18499233 (-)
RNA-Seq ExpressionPI0016064
SyntenyPI0016064
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTTTAAAAAAGCTGACGTCACAGTACCTTTGACTCTTTCTCTCTCTTCCTTTAAACTCAAACCCACCTCTGTTCTACGTCTGCGACTCACTCTTTTCTCTCTCTCTCGTCCCACGATTTTTTTTCTCTTTCAGATTCTTCTCTCCGGCGGTGGCGTCGGTGGAGGAACCGGTGGTTTACTTTCACGGAAGATGGTGAAAGATGACTTGTGTATTACAATTCCCAGCGATTTCAGGTGTCCGATCTCGTTAGACGTTATGAAATCCCCTGTAAGTTTATGTACCGGAGTGACCTACGATCGTTCCAGTATTCAGAAATGGCTTGATAATGGAAACAACACTTGTCCGGCCACCATGCAGGTCCTTCAAACCAAGGAGTTCGTACCTAACCACAATCTCCACCGTCTCATTCAGATTTGGTCCGATTCCCTTCACCAACGCCTTCACTCCCCTGTTTCCGACTCTTCTCTCTCCTCCGATCAAGTTCTCCGCCTCATCAACCGTATGGATGACCATCAATCACACCCTGATTCTCTTACGCGGCTTGTCTCTTTCGCTTCTGAATCGGTGGACAATCGGAGGCTTCTCTGTGGAATTGACAGACTCCTGCCGGTACTTGTCGATCTTCTTTGCAATGTCGATGCCGGTGATTTGGTTGACCAAACGGTGAGGTTGTTGCATTTGATTCGGAGTGAGATTGGGGATAAGGAGAGGTTTGTGAAGATGATGTTGAATACCGATCGTAATTGTATTTCATCTCTGCTTTTGATTCTGCGGAAAGGAACCGTCGAATTGAAAATCTGTTCTGCTAATCTGTTGGAATATCTCGCAATCGACGCCGAAGCCAAGATTGTAATTGCAGAAACAGATGGATTGATGCAAGAATTACTCAAATTAATCAATTCTGAAAACGATACAACCCTAATTGAATCCCTTCTATCATGCCTAATTTCGATTTCAATGCCGAAACGGATCAAAATCAAATTAGTCCAACTCGGAGTGATAAAATCAGTAACGAAGCTTCTCTCGGAATCGAAGTCGAGTTCAAGCATTTCGATGACGGAGAAGGTACTGAAAGTTCTAGAAACGGCATCGACGGTGAGAGAAGGAAGAACAGAGATCGGAGAAGACTCCGTATGCGTAGCGGCGATAGTACAGAAAGTGTTGAAGGTATCGAACGCCGCGACGGAGCACGCGGTGACGACGCTGTGGAGCGTTTGTTATCTGTTCAGAGACGAGAAGGCGGCAGAGGCGTTGACGAAGGCGAATGGATTAACGAAGATTTTGTTGTTAATGCAGAGCAATTGCTCACCGCCGGTACGGCAGATGGCGAGAGATCTGTTGAAGATTTTTCGGATCAATTCCAAATCGTGCCTATCGAGCTACGATACAAAAACTACGCATATCATGCCATGTTAAAGCCGATTTGGTTTCTTCATCATCATCTCCCTCTTCTTCTTCCTCCATTTCAAACCATTTCGATTTCTCTTTGTAAGTTATTATGGAGATCTCTGGGGAGAGATTTTACTTTTCTTTTTCTTTTCTTTTCTTTTCTTTTAATGTTCTTGTAAATCAAAGAAATTTTGTCAGTGAATACAAACAAAATTATTCGATTTTCAACTTCTGTTCTTGCATTTCCGTGTAAGCCTCATCGGATTCAGTTCTTTTTTTTTAGGTTTGTAATATTTACCTAAGCAAAAAAATATTCAATTAGTTAAAAATACAATTCTAGTCATCGTTCCATTTTTTTTTTTTCTTTCTCTAGAAAACATTTCGTTTTATTCCTAATAAATGTCTCTCTCTCTCTCTCTCTTTTTAAGTATAACATACTGGGACAAGAGATTGTG

mRNA sequence

ATTTTTTTAAAAAAGCTGACGTCACAGTACCTTTGACTCTTTCTCTCTCTTCCTTTAAACTCAAACCCACCTCTGTTCTACGTCTGCGACTCACTCTTTTCTCTCTCTCTCGTCCCACGATTTTTTTTCTCTTTCAGATTCTTCTCTCCGGCGGTGGCGTCGGTGGAGGAACCGGTGGTTTACTTTCACGGAAGATGGTGAAAGATGACTTGTGTATTACAATTCCCAGCGATTTCAGGTGTCCGATCTCGTTAGACGTTATGAAATCCCCTGTAAGTTTATGTACCGGAGTGACCTACGATCGTTCCAGTATTCAGAAATGGCTTGATAATGGAAACAACACTTGTCCGGCCACCATGCAGGTCCTTCAAACCAAGGAGTTCGTACCTAACCACAATCTCCACCGTCTCATTCAGATTTGGTCCGATTCCCTTCACCAACGCCTTCACTCCCCTGTTTCCGACTCTTCTCTCTCCTCCGATCAAGTTCTCCGCCTCATCAACCGTATGGATGACCATCAATCACACCCTGATTCTCTTACGCGGCTTGTCTCTTTCGCTTCTGAATCGGTGGACAATCGGAGGCTTCTCTGTGGAATTGACAGACTCCTGCCGGTACTTGTCGATCTTCTTTGCAATGTCGATGCCGGTGATTTGGTTGACCAAACGGTGAGGTTGTTGCATTTGATTCGGAGTGAGATTGGGGATAAGGAGAGGTTTGTGAAGATGATGTTGAATACCGATCGTAATTGTATTTCATCTCTGCTTTTGATTCTGCGGAAAGGAACCGTCGAATTGAAAATCTGTTCTGCTAATCTGTTGGAATATCTCGCAATCGACGCCGAAGCCAAGATTGTAATTGCAGAAACAGATGGATTGATGCAAGAATTACTCAAATTAATCAATTCTGAAAACGATACAACCCTAATTGAATCCCTTCTATCATGCCTAATTTCGATTTCAATGCCGAAACGGATCAAAATCAAATTAGTCCAACTCGGAGTGATAAAATCAGTAACGAAGCTTCTCTCGGAATCGAAGTCGAGTTCAAGCATTTCGATGACGGAGAAGGTACTGAAAGTTCTAGAAACGGCATCGACGGTGAGAGAAGGAAGAACAGAGATCGGAGAAGACTCCGTATGCGTAGCGGCGATAGTACAGAAAGTGTTGAAGGTATCGAACGCCGCGACGGAGCACGCGGTGACGACGCTGTGGAGCGTTTGTTATCTGTTCAGAGACGAGAAGGCGGCAGAGGCGTTGACGAAGGCGAATGGATTAACGAAGATTTTGTTGTTAATGCAGAGCAATTGCTCACCGCCGGTACGGCAGATGGCGAGAGATCTGTTGAAGATTTTTCGGATCAATTCCAAATCGTGCCTATCGAGCTACGATACAAAAACTACGCATATCATGCCATGTTAAAGCCGATTTGGTTTCTTCATCATCATCTCCCTCTTCTTCTTCCTCCATTTCAAACCATTTCGATTTCTCTTTGTAAGTTATTATGGAGATCTCTGGGGAGAGATTTTACTTTTCTTTTTCTTTTCTTTTCTTTTCTTTTAATGTTCTTGTAAATCAAAGAAATTTTGTCAGTGAATACAAACAAAATTATTCGATTTTCAACTTCTGTTCTTGCATTTCCGTGTAAGCCTCATCGGATTCAGTTCTTTTTTTTTAGGTTTGTAATATTTACCTAAGCAAAAAAATATTCAATTAGTTAAAAATACAATTCTAGTCATCGTTCCATTTTTTTTTTTTCTTTCTCTAGAAAACATTTCGTTTTATTCCTAATAAATGTCTCTCTCTCTCTCTCTCTTTTTAAGTATAACATACTGGGACAAGAGATTGTG

Coding sequence (CDS)

ATGGTGAAAGATGACTTGTGTATTACAATTCCCAGCGATTTCAGGTGTCCGATCTCGTTAGACGTTATGAAATCCCCTGTAAGTTTATGTACCGGAGTGACCTACGATCGTTCCAGTATTCAGAAATGGCTTGATAATGGAAACAACACTTGTCCGGCCACCATGCAGGTCCTTCAAACCAAGGAGTTCGTACCTAACCACAATCTCCACCGTCTCATTCAGATTTGGTCCGATTCCCTTCACCAACGCCTTCACTCCCCTGTTTCCGACTCTTCTCTCTCCTCCGATCAAGTTCTCCGCCTCATCAACCGTATGGATGACCATCAATCACACCCTGATTCTCTTACGCGGCTTGTCTCTTTCGCTTCTGAATCGGTGGACAATCGGAGGCTTCTCTGTGGAATTGACAGACTCCTGCCGGTACTTGTCGATCTTCTTTGCAATGTCGATGCCGGTGATTTGGTTGACCAAACGGTGAGGTTGTTGCATTTGATTCGGAGTGAGATTGGGGATAAGGAGAGGTTTGTGAAGATGATGTTGAATACCGATCGTAATTGTATTTCATCTCTGCTTTTGATTCTGCGGAAAGGAACCGTCGAATTGAAAATCTGTTCTGCTAATCTGTTGGAATATCTCGCAATCGACGCCGAAGCCAAGATTGTAATTGCAGAAACAGATGGATTGATGCAAGAATTACTCAAATTAATCAATTCTGAAAACGATACAACCCTAATTGAATCCCTTCTATCATGCCTAATTTCGATTTCAATGCCGAAACGGATCAAAATCAAATTAGTCCAACTCGGAGTGATAAAATCAGTAACGAAGCTTCTCTCGGAATCGAAGTCGAGTTCAAGCATTTCGATGACGGAGAAGGTACTGAAAGTTCTAGAAACGGCATCGACGGTGAGAGAAGGAAGAACAGAGATCGGAGAAGACTCCGTATGCGTAGCGGCGATAGTACAGAAAGTGTTGAAGGTATCGAACGCCGCGACGGAGCACGCGGTGACGACGCTGTGGAGCGTTTGTTATCTGTTCAGAGACGAGAAGGCGGCAGAGGCGTTGACGAAGGCGAATGGATTAACGAAGATTTTGTTGTTAATGCAGAGCAATTGCTCACCGCCGGTACGGCAGATGGCGAGAGATCTGTTGAAGATTTTTCGGATCAATTCCAAATCGTGCCTATCGAGCTACGATACAAAAACTACGCATATCATGCCATGTTAA

Protein sequence

MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQTKEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Homology
BLAST of PI0016064 vs. ExPASy Swiss-Prot
Match: Q9FLF4 (U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-120
Identity = 232/421 (55.11%), Postives = 315/421 (74.82%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           M KDDLCIT+P+ FRCPISLDVMKSPVSLCTGVTYDR+SIQ+WLD GNNTCPATMQ+LQ 
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSS---DQVLRLINRMDDHQSHPDS--- 120
           K+F+PN  L RLI+IWSDS+ +R  + V  + L++   D++   I+R+   +   D    
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRR--TCVESAELAAPTRDEIADAIDRVKIEKEERDDREV 120

Query: 121 LTRLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVD-------AGDLVDQTVRLLHLIRS 180
           L+++V F  ES DNR  L G D  + +LVDL+  VD          +V + V++L  IRS
Sbjct: 121 LSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRS 180

Query: 181 EIGDKERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDG 240
           ++ D+ RF  ++L   R+ +S ++ + + G VELKI  A LLE++A+DAE+K++IAE DG
Sbjct: 181 KVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDG 240

Query: 241 LMQELLKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSI 300
           L+ EL+K I+ ++D +LIES LSCLI+IS PKR+K+ L++  +I  VTKLLS+S SS S+
Sbjct: 241 LITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSV 300

Query: 301 SMTEKVLKVLETASTVREGRTEI-GEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLF 360
           S+TEK LK+LE  ++ +EGR+EI G D  C+  +V+K++KVS AATEHAVT LWSV YLF
Sbjct: 301 SVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLF 360

Query: 361 RDEKAAEALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIM 408
           +++KA EA+T  NG+TKILLL+QSNCSP VR+M  DLLK+F++NS+SCLS+YDTKTTHIM
Sbjct: 361 KEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNSRSCLSAYDTKTTHIM 419

BLAST of PI0016064 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-106
Identity = 213/415 (51.33%), Postives = 307/415 (73.98%), Query Frame = 0

Query: 1   MVKDDLCI-TIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQ 60
           M  DDL I T+P  F+CPISLDVMKSPVSL TGVTYDR SIQ+WLD+GNNTCPATMQ+LQ
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 61  TKEFVPNHNLHRLIQIWSDSLHQRL--HSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTR 120
            KEFVPN  LHRLI  WSDS+++R    SP SD+  + D++   I R    +   D+ ++
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRRADSESPESDTP-TRDEINAAIERF---RIENDARSK 120

Query: 121 LVSFASESVDNRRLLCGIDRLLPVLVDLLCNV-DAGD----LVDQTVRLLHLIRSEIGDK 180
           ++ FA ES +NR  L G D  + +LVDL+ +  +  D    LV + V++L +IR +I D+
Sbjct: 121 ILRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDR 180

Query: 181 ERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQEL 240
            R   ++L    +C++S  L++++G  +LKI  + +LE++A+DAE+K++IA+ +GL+ E+
Sbjct: 181 RRLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKGEGLVTEI 240

Query: 241 LKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEK 300
           +KLI+S++D++LIE+ LS LI+I+  KR+K+ L++  ++  +T LL++   ++S+S+TEK
Sbjct: 241 IKLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTD--PTTSVSVTEK 300

Query: 301 VLKVLETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA 360
            LK+LE  S+ +EGR+EI  D VCV  +V K++KVS AATEHAVT LWSVCYLF+++KA 
Sbjct: 301 CLKLLEAISSCKEGRSEIC-DGVCVETVVNKLMKVSTAATEHAVTVLWSVCYLFKEKKAQ 360

Query: 361 EALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMP 408
           +A+ + NG+TKILLL+QSNCS  VR M  DLLK+F++NS+SCLS Y+TKTTHIMP
Sbjct: 361 DAVIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNSRSCLSVYETKTTHIMP 408

BLAST of PI0016064 vs. ExPASy Swiss-Prot
Match: Q9LSA6 (U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 7.1e-90
Identity = 193/414 (46.62%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 8   ITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQTKEFVPNH 67
           IT+PS F+CPISLDVM+SPVSLCTGVTYDR+SIQ+WLD GNNTCPATMQ+L+TK+FVPN 
Sbjct: 10  ITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPNL 69

Query: 68  NLHRLIQIWSDSLHQR-------LHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 127
            L RLI IWSDS+ +R       L+ P      + ++V  L+ R+       ++L ++V 
Sbjct: 70  TLQRLINIWSDSIGRRHNGDSPVLNPPSGREVPTKEEVNVLLERL----MSLENLMKIVR 129

Query: 128 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAG-DLVDQTVRLLHLIRSEIGDKERFVKMM 187
           F  +S  NR  L      +P+LVD++       +LV   +R+L  I+    D+ER   +M
Sbjct: 130 FVKDSDSNREFLSKKMEFVPMLVDIIRTKKTKIELVIMAIRILDSIKV---DRERLSNLM 189

Query: 188 L-NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLIN- 247
           L N   +C++++LL +++G +E KI S  +L++++ DA++K++IAE DG++ E++K I+ 
Sbjct: 190 LANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSISI 249

Query: 248 -SENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 307
              +D +LIE+ LS LI+IS  KR++ KL+    I  +  +L  +++ +++++TEK LK+
Sbjct: 250 TESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILL-TETLTNVAVTEKSLKL 309

Query: 308 LETASTVREGRTEI-GEDS-VCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA-E 367
           LET S+ REGR EI G+D+  CV  +V+K+LKVS  ATEHAVT LW +CY+FR++K   E
Sbjct: 310 LETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKTVEE 369

Query: 368 ALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMP 408
            + ++NG+TK+L+++QSNCS  VRQMA+DL+K+ + NS S L++Y+TKTTHIMP
Sbjct: 370 TVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFNS-SALAAYETKTTHIMP 414

BLAST of PI0016064 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 8.6e-35
Identity = 111/387 (28.68%), Postives = 192/387 (49.61%), Query Frame = 0

Query: 8   ITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNG-NNTCPATMQVLQTKEFVPN 67
           I IP  F CPISL++MK PV + TG+TYDR SI+KWL  G  N+CP T Q +   +  PN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 68  HNLHRLIQIWS--DSLHQRLHSPVSDSSLSSDQVLRLI-NRMDDHQSHPDSLTRLVSFAS 127
           H L RLIQ W   ++ +     P     +   ++ +LI +    H++    L RL    S
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 128 ESVDNRRLL--CGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMMLN 187
           E+  N+R L   G+   L  +V    + + G L D+ + LL+ + +     E  +K +LN
Sbjct: 130 ENATNKRCLEAAGVPEFLANIVS--NDSENGSLTDEALNLLYHLET----SETVLKNLLN 189

Query: 188 T--DRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSE 247
              D N + SL  I+++G  E ++ +  LL+ +   A+    +     +  E++++++  
Sbjct: 190 NKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDR 249

Query: 248 NDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLET 307
                 ++ +  L++I    R + K V+ GVI  + +LL +   +S     E  + VL+ 
Sbjct: 250 ISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDL 309

Query: 308 ASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKAN 367
                EGR E       +A + +K+L+VS  A++ AV  L SV            + +  
Sbjct: 310 LCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLG 369

Query: 368 GLTKILLLMQSNCSPPVRQMARDLLKI 387
            + K+ L++Q +C    ++ A++LLK+
Sbjct: 370 VVAKLCLVLQVSCGGKTKEKAKELLKL 390

BLAST of PI0016064 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-31
Identity = 113/394 (28.68%), Postives = 200/394 (50.76%), Query Frame = 0

Query: 4   DDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQTKEF 63
           +++ ITIPS F+CPIS ++MK PV + +G+TYDR +I+KW ++G  TCP T  VL + E 
Sbjct: 27  EEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQ 86

Query: 64  VPNHNLHRLIQIW-SDSLHQRLHS-PVSDSSLSSDQVLRLINRMD------DHQSHPDSL 123
           +PNH + R+IQ W   SL   +   P     ++S QV  +  R+       D+ +  + +
Sbjct: 87  IPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMV 146

Query: 124 TRLVSFASESVDNRRLL--CGIDRLLPVLVDLLC-NVDAGDLVDQTVRLLHLIRSEIGDK 183
           T++     ES  NR+ +   G   +L V  D    N +A  L+++TV +L  +   IG  
Sbjct: 147 TKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWM-LPIG-L 206

Query: 184 ERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQEL 243
           E   K+   T  +  + L+ +LR G          LLE       A   + + +G+ +  
Sbjct: 207 EGQSKL---TTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHA---LTKINGVQEAF 266

Query: 244 LKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEK 303
           +K IN   D+T + SL+S    I   +    + ++L ++    ++L +S++    S+ EK
Sbjct: 267 MKSIN--RDSTCVNSLISIHHMILTNQETVSRFLELDLVNITVEMLVDSEN----SVCEK 326

Query: 304 VLKVLETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA 363
            L VL      +EGR ++  + + +  +V+K+LK+S    +  V+ +W VC      +  
Sbjct: 327 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCKSGDGSEVE 386

Query: 364 EALTKANGLTKILLLMQSNCSPPVRQMARDLLKI 387
           EAL +     K+++++Q  C    ++   +LLK+
Sbjct: 387 EAL-RLGAFKKLVVMLQVGCGEGTKEKVTELLKM 403

BLAST of PI0016064 vs. ExPASy TrEMBL
Match: A0A5A7SMT0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00310 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.0e-211
Identity = 390/408 (95.59%), Postives = 397/408 (97.30%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           KEFVPNHNLHRLIQIWSDS HQRLHSPVSDSSLSS Q+LRLINRMDDH+S  DSL RLVS
Sbjct: 61  KEFVPNHNLHRLIQIWSDSHHQRLHSPVSDSSLSSHQILRLINRMDDHRSRADSLPRLVS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASESV+NRRLLCGID L+PVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVK ML
Sbjct: 121 FASESVENRRLLCGIDGLVPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKTML 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
           N+DRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLM+ELLKLINSEN
Sbjct: 181 NSDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMRELLKLINSEN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTT+IES LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSSISMTEKVLKVLETA
Sbjct: 241 DTTVIESALSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSISMTEKVLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGR EIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRKEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 408

BLAST of PI0016064 vs. ExPASy TrEMBL
Match: A0A1S3B2G8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485262 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.0e-211
Identity = 390/408 (95.59%), Postives = 397/408 (97.30%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           KEFVPNHNLHRLIQIWSDS HQRLHSPVSDSSLSS Q+LRLINRMDDH+S  DSL RLVS
Sbjct: 61  KEFVPNHNLHRLIQIWSDSHHQRLHSPVSDSSLSSHQILRLINRMDDHRSRADSLPRLVS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASESV+NRRLLCGID L+PVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVK ML
Sbjct: 121 FASESVENRRLLCGIDGLVPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKTML 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
           N+DRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLM+ELLKLINSEN
Sbjct: 181 NSDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMRELLKLINSEN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTT+IES LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSSISMTEKVLKVLETA
Sbjct: 241 DTTVIESALSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSISMTEKVLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGR EIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRKEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 408

BLAST of PI0016064 vs. ExPASy TrEMBL
Match: A0A0A0KJ12 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G484010 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 5.0e-203
Identity = 378/408 (92.65%), Postives = 392/408 (96.08%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIP+DFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPTDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           K+FVPNHNLHRLIQIWSDSLHQRLHSP+SDSSLSSDQ+LRLIN    HQS  DSL RL+S
Sbjct: 61  KDFVPNHNLHRLIQIWSDSLHQRLHSPLSDSSLSSDQLLRLIN----HQSRADSLPRLLS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASES+DNRRLL GID LLP+LVDLLCNV AGDL+DQ VRLLHLIR+EIGDKERFVK +L
Sbjct: 121 FASESLDNRRLLSGIDGLLPLLVDLLCNVHAGDLLDQAVRLLHLIRTEIGDKERFVKTIL 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
           NTDRN ISSLLLILRKGTVELKICSANLLEYLAIDAEAKI+IAETDGLMQELLKLINS+N
Sbjct: 181 NTDRNSISSLLLILRKGTVELKICSANLLEYLAIDAEAKILIAETDGLMQELLKLINSQN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTTLIES+LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSS SMTEK+LKVLETA
Sbjct: 241 DTTLIESVLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSSSMTEKLLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 404

BLAST of PI0016064 vs. ExPASy TrEMBL
Match: A0A6J1GDY4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453282 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 8.0e-193
Identity = 361/412 (87.62%), Postives = 382/412 (92.72%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLH----SPVSDSSLSSDQVLRLINRMDDHQSHPDSLT 120
           KEFVPNHNLHRLIQIWSDSL QRLH    SPVSD++LSSD VLRL+ RM+D +   DSL+
Sbjct: 61  KEFVPNHNLHRLIQIWSDSLRQRLHSPDSSPVSDTALSSDHVLRLVKRMEDDKLRADSLS 120

Query: 121 RLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFV 180
           RLVSFA++SV+NRRLLCGID L+PVLVDLL NVD   LV QTVRLLHLIR+EI DKERFV
Sbjct: 121 RLVSFAAQSVENRRLLCGIDGLVPVLVDLLGNVDGDVLVAQTVRLLHLIRNEIEDKERFV 180

Query: 181 KMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLI 240
           KMML +DRNCI SLLLILRK TVELKICS NLLE+LAIDAEAKI IAETDGLMQ+LLKL+
Sbjct: 181 KMMLKSDRNCIWSLLLILRKETVELKICSVNLLEHLAIDAEAKIAIAETDGLMQDLLKLM 240

Query: 241 NSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 300
           NSEND+ LIES+LSCLISISMPKRIKIKLV LGVIKS TKLLSE+ SSSSISMTEKVLKV
Sbjct: 241 NSENDSALIESVLSCLISISMPKRIKIKLVHLGVIKSATKLLSEANSSSSISMTEKVLKV 300

Query: 301 LETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALT 360
           LETASTVREGRTEIGEDS C+AAIVQKVLKVSN ATEH+VTTLWS+CYLFRDEKAAEALT
Sbjct: 301 LETASTVREGRTEIGEDSACIAAIVQKVLKVSNTATEHSVTTLWSICYLFRDEKAAEALT 360

Query: 361 KANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           KANGLTKILLLMQSNCS PVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 KANGLTKILLLMQSNCSAPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 412

BLAST of PI0016064 vs. ExPASy TrEMBL
Match: A0A6J1IPD9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478747 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.3e-192
Identity = 360/412 (87.38%), Postives = 381/412 (92.48%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLH----SPVSDSSLSSDQVLRLINRMDDHQSHPDSLT 120
           KEFVPNHNLHRLIQIWSDSL QRLH    SPVSD++LSSD  LRL+ RM+D +   DSL+
Sbjct: 61  KEFVPNHNLHRLIQIWSDSLRQRLHSPDSSPVSDAALSSDHALRLVKRMEDDKLRADSLS 120

Query: 121 RLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFV 180
           RLVSFA++SV+N+RLLCGID L+PVLVDLL NVD   LV QTVRLLHLIR+EI DKERF 
Sbjct: 121 RLVSFAAQSVENQRLLCGIDGLVPVLVDLLGNVDGDVLVAQTVRLLHLIRNEIEDKERFA 180

Query: 181 KMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLI 240
           K ML +DRNCISSLLLILRK TVELKICS NLLE+LAIDAEAKI IAETDGLMQ+LLKL+
Sbjct: 181 KTMLKSDRNCISSLLLILRKETVELKICSVNLLEHLAIDAEAKIAIAETDGLMQDLLKLM 240

Query: 241 NSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 300
           NSEND+ LIES+LSCLISISMPKRIKIKLV LGVIKSVTKLLSE+ SSSSISMTEKVLKV
Sbjct: 241 NSENDSALIESVLSCLISISMPKRIKIKLVHLGVIKSVTKLLSEANSSSSISMTEKVLKV 300

Query: 301 LETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALT 360
           LETASTVREGRTEIGEDS C+AAIVQKVLKVSN ATEHAVTTLWS+CYLFRDEKAAEALT
Sbjct: 301 LETASTVREGRTEIGEDSACIAAIVQKVLKVSNTATEHAVTTLWSICYLFRDEKAAEALT 360

Query: 361 KANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           KANGLTKILLLMQSNCS PVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 KANGLTKILLLMQSNCSAPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 412

BLAST of PI0016064 vs. NCBI nr
Match: XP_008441024.1 (PREDICTED: U-box domain-containing protein 27 [Cucumis melo] >KAA0025541.1 U-box domain-containing protein 27 [Cucumis melo var. makuwa] >TYK25700.1 U-box domain-containing protein 27 [Cucumis melo var. makuwa])

HSP 1 Score: 743.4 bits (1918), Expect = 1.0e-210
Identity = 390/408 (95.59%), Postives = 397/408 (97.30%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           KEFVPNHNLHRLIQIWSDS HQRLHSPVSDSSLSS Q+LRLINRMDDH+S  DSL RLVS
Sbjct: 61  KEFVPNHNLHRLIQIWSDSHHQRLHSPVSDSSLSSHQILRLINRMDDHRSRADSLPRLVS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASESV+NRRLLCGID L+PVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVK ML
Sbjct: 121 FASESVENRRLLCGIDGLVPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKTML 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
           N+DRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLM+ELLKLINSEN
Sbjct: 181 NSDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMRELLKLINSEN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTT+IES LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSSISMTEKVLKVLETA
Sbjct: 241 DTTVIESALSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSISMTEKVLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGR EIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRKEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 408

BLAST of PI0016064 vs. NCBI nr
Match: XP_038883742.1 (U-box domain-containing protein 27 [Benincasa hispida])

HSP 1 Score: 718.0 bits (1852), Expect = 4.6e-203
Identity = 380/408 (93.14%), Postives = 390/408 (95.59%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MV+DDLCITIP+DFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVRDDLCITIPNDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           KEFVPNHNLHRLIQIWSDSL QRLHSPVSDSSLSSDQVLRL+NRM+D QS  D+L RLVS
Sbjct: 61  KEFVPNHNLHRLIQIWSDSLRQRLHSPVSDSSLSSDQVLRLVNRMEDDQSRADTLPRLVS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASESV+NRRLLCGID L+ VLVDLL NVD G LV+QTVRLLHLIRSEI DKERFVK ML
Sbjct: 121 FASESVENRRLLCGIDGLVSVLVDLLGNVDGGVLVEQTVRLLHLIRSEIEDKERFVKTML 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
            +DRNCISSLLLILRK TVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN
Sbjct: 181 KSDRNCISSLLLILRKATVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTTLIES+LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSSISMTEKVLKVLETA
Sbjct: 241 DTTLIESVLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSISMTEKVLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGRTEIGEDSVCVAAIVQKVLKVSN ATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNTATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCS PVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSAPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 408

BLAST of PI0016064 vs. NCBI nr
Match: XP_004148672.1 (U-box domain-containing protein 27 [Cucumis sativus] >KGN48372.1 hypothetical protein Csa_002868 [Cucumis sativus])

HSP 1 Score: 716.8 bits (1849), Expect = 1.0e-202
Identity = 378/408 (92.65%), Postives = 392/408 (96.08%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIP+DFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPTDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 120
           K+FVPNHNLHRLIQIWSDSLHQRLHSP+SDSSLSSDQ+LRLIN    HQS  DSL RL+S
Sbjct: 61  KDFVPNHNLHRLIQIWSDSLHQRLHSPLSDSSLSSDQLLRLIN----HQSRADSLPRLLS 120

Query: 121 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMML 180
           FASES+DNRRLL GID LLP+LVDLLCNV AGDL+DQ VRLLHLIR+EIGDKERFVK +L
Sbjct: 121 FASESLDNRRLLSGIDGLLPLLVDLLCNVHAGDLLDQAVRLLHLIRTEIGDKERFVKTIL 180

Query: 181 NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSEN 240
           NTDRN ISSLLLILRKGTVELKICSANLLEYLAIDAEAKI+IAETDGLMQELLKLINS+N
Sbjct: 181 NTDRNSISSLLLILRKGTVELKICSANLLEYLAIDAEAKILIAETDGLMQELLKLINSQN 240

Query: 241 DTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLETA 300
           DTTLIES+LSCLISISMPKRIKIKLVQLGVIKSVTKLLSES SSSS SMTEK+LKVLETA
Sbjct: 241 DTTLIESVLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESNSSSSSSMTEKLLKVLETA 300

Query: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKANG 360
           STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKA EALTKANG
Sbjct: 301 STVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAGEALTKANG 360

Query: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 LTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 404

BLAST of PI0016064 vs. NCBI nr
Match: KAG7034432.1 (U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 683.3 bits (1762), Expect = 1.3e-192
Identity = 361/412 (87.62%), Postives = 382/412 (92.72%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLH----SPVSDSSLSSDQVLRLINRMDDHQSHPDSLT 120
           KEFVPNHNLHRLIQIWSDS  QRLH    SPVSDS+LSSD VLRL+ RM+D +   DSL+
Sbjct: 61  KEFVPNHNLHRLIQIWSDSFRQRLHSPDSSPVSDSALSSDHVLRLLKRMEDDKLRADSLS 120

Query: 121 RLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFV 180
           RLVSFA++SV+NRRLLCGID L+PVLVDLL NVD   LV QTVR+LHLIR+EI DKERFV
Sbjct: 121 RLVSFAAQSVENRRLLCGIDGLVPVLVDLLGNVDGDVLVAQTVRMLHLIRNEIEDKERFV 180

Query: 181 KMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLI 240
           KMML +DRNCISSLLLILRK TVELKICS NLLE+LAIDAEAKI IAETDGLMQ+LLKL+
Sbjct: 181 KMMLKSDRNCISSLLLILRKETVELKICSVNLLEHLAIDAEAKIAIAETDGLMQDLLKLM 240

Query: 241 NSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 300
           NSEND+ LIES+LSCLISISMPKRIKIKLV LGVIKSVTKLLSE+ SSSSISMTEKVLKV
Sbjct: 241 NSENDSALIESVLSCLISISMPKRIKIKLVHLGVIKSVTKLLSEANSSSSISMTEKVLKV 300

Query: 301 LETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALT 360
           LETASTVREGRTEIGEDS C+AAIVQKVLKVSN ATEH+VTTLWS+CYLFRDEKAAEALT
Sbjct: 301 LETASTVREGRTEIGEDSACIAAIVQKVLKVSNTATEHSVTTLWSICYLFRDEKAAEALT 360

Query: 361 KANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           KANGLTKILLLMQSNCS PVRQMARDLLKIFRI SKSCLSSYDTKTTHIMPC
Sbjct: 361 KANGLTKILLLMQSNCSAPVRQMARDLLKIFRITSKSCLSSYDTKTTHIMPC 412

BLAST of PI0016064 vs. NCBI nr
Match: XP_022950098.1 (U-box domain-containing protein 27 [Cucurbita moschata])

HSP 1 Score: 682.9 bits (1761), Expect = 1.6e-192
Identity = 361/412 (87.62%), Postives = 382/412 (92.72%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT
Sbjct: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLH----SPVSDSSLSSDQVLRLINRMDDHQSHPDSLT 120
           KEFVPNHNLHRLIQIWSDSL QRLH    SPVSD++LSSD VLRL+ RM+D +   DSL+
Sbjct: 61  KEFVPNHNLHRLIQIWSDSLRQRLHSPDSSPVSDTALSSDHVLRLVKRMEDDKLRADSLS 120

Query: 121 RLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFV 180
           RLVSFA++SV+NRRLLCGID L+PVLVDLL NVD   LV QTVRLLHLIR+EI DKERFV
Sbjct: 121 RLVSFAAQSVENRRLLCGIDGLVPVLVDLLGNVDGDVLVAQTVRLLHLIRNEIEDKERFV 180

Query: 181 KMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLI 240
           KMML +DRNCI SLLLILRK TVELKICS NLLE+LAIDAEAKI IAETDGLMQ+LLKL+
Sbjct: 181 KMMLKSDRNCIWSLLLILRKETVELKICSVNLLEHLAIDAEAKIAIAETDGLMQDLLKLM 240

Query: 241 NSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 300
           NSEND+ LIES+LSCLISISMPKRIKIKLV LGVIKS TKLLSE+ SSSSISMTEKVLKV
Sbjct: 241 NSENDSALIESVLSCLISISMPKRIKIKLVHLGVIKSATKLLSEANSSSSISMTEKVLKV 300

Query: 301 LETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALT 360
           LETASTVREGRTEIGEDS C+AAIVQKVLKVSN ATEH+VTTLWS+CYLFRDEKAAEALT
Sbjct: 301 LETASTVREGRTEIGEDSACIAAIVQKVLKVSNTATEHSVTTLWSICYLFRDEKAAEALT 360

Query: 361 KANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 409
           KANGLTKILLLMQSNCS PVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC
Sbjct: 361 KANGLTKILLLMQSNCSAPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMPC 412

BLAST of PI0016064 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 434.9 bits (1117), Expect = 7.3e-122
Identity = 232/421 (55.11%), Postives = 315/421 (74.82%), Query Frame = 0

Query: 1   MVKDDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQT 60
           M KDDLCIT+P+ FRCPISLDVMKSPVSLCTGVTYDR+SIQ+WLD GNNTCPATMQ+LQ 
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 61  KEFVPNHNLHRLIQIWSDSLHQRLHSPVSDSSLSS---DQVLRLINRMDDHQSHPDS--- 120
           K+F+PN  L RLI+IWSDS+ +R  + V  + L++   D++   I+R+   +   D    
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRR--TCVESAELAAPTRDEIADAIDRVKIEKEERDDREV 120

Query: 121 LTRLVSFASESVDNRRLLCGIDRLLPVLVDLLCNVD-------AGDLVDQTVRLLHLIRS 180
           L+++V F  ES DNR  L G D  + +LVDL+  VD          +V + V++L  IRS
Sbjct: 121 LSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRS 180

Query: 181 EIGDKERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDG 240
           ++ D+ RF  ++L   R+ +S ++ + + G VELKI  A LLE++A+DAE+K++IAE DG
Sbjct: 181 KVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDG 240

Query: 241 LMQELLKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSI 300
           L+ EL+K I+ ++D +LIES LSCLI+IS PKR+K+ L++  +I  VTKLLS+S SS S+
Sbjct: 241 LITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSV 300

Query: 301 SMTEKVLKVLETASTVREGRTEI-GEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLF 360
           S+TEK LK+LE  ++ +EGR+EI G D  C+  +V+K++KVS AATEHAVT LWSV YLF
Sbjct: 301 SVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLF 360

Query: 361 RDEKAAEALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIM 408
           +++KA EA+T  NG+TKILLL+QSNCSP VR+M  DLLK+F++NS+SCLS+YDTKTTHIM
Sbjct: 361 KEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNSRSCLSAYDTKTTHIM 419

BLAST of PI0016064 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.3e-107
Identity = 213/415 (51.33%), Postives = 307/415 (73.98%), Query Frame = 0

Query: 1   MVKDDLCI-TIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQ 60
           M  DDL I T+P  F+CPISLDVMKSPVSL TGVTYDR SIQ+WLD+GNNTCPATMQ+LQ
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 61  TKEFVPNHNLHRLIQIWSDSLHQRL--HSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTR 120
            KEFVPN  LHRLI  WSDS+++R    SP SD+  + D++   I R    +   D+ ++
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRRADSESPESDTP-TRDEINAAIERF---RIENDARSK 120

Query: 121 LVSFASESVDNRRLLCGIDRLLPVLVDLLCNV-DAGD----LVDQTVRLLHLIRSEIGDK 180
           ++ FA ES +NR  L G D  + +LVDL+ +  +  D    LV + V++L +IR +I D+
Sbjct: 121 ILRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDR 180

Query: 181 ERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQEL 240
            R   ++L    +C++S  L++++G  +LKI  + +LE++A+DAE+K++IA+ +GL+ E+
Sbjct: 181 RRLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKGEGLVTEI 240

Query: 241 LKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEK 300
           +KLI+S++D++LIE+ LS LI+I+  KR+K+ L++  ++  +T LL++   ++S+S+TEK
Sbjct: 241 IKLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTD--PTTSVSVTEK 300

Query: 301 VLKVLETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA 360
            LK+LE  S+ +EGR+EI  D VCV  +V K++KVS AATEHAVT LWSVCYLF+++KA 
Sbjct: 301 CLKLLEAISSCKEGRSEIC-DGVCVETVVNKLMKVSTAATEHAVTVLWSVCYLFKEKKAQ 360

Query: 361 EALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMP 408
           +A+ + NG+TKILLL+QSNCS  VR M  DLLK+F++NS+SCLS Y+TKTTHIMP
Sbjct: 361 DAVIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNSRSCLSVYETKTTHIMP 408

BLAST of PI0016064 vs. TAIR 10
Match: AT3G18710.1 (plant U-box 29 )

HSP 1 Score: 332.4 bits (851), Expect = 5.1e-91
Identity = 193/414 (46.62%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 8   ITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQTKEFVPNH 67
           IT+PS F+CPISLDVM+SPVSLCTGVTYDR+SIQ+WLD GNNTCPATMQ+L+TK+FVPN 
Sbjct: 10  ITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPNL 69

Query: 68  NLHRLIQIWSDSLHQR-------LHSPVSDSSLSSDQVLRLINRMDDHQSHPDSLTRLVS 127
            L RLI IWSDS+ +R       L+ P      + ++V  L+ R+       ++L ++V 
Sbjct: 70  TLQRLINIWSDSIGRRHNGDSPVLNPPSGREVPTKEEVNVLLERL----MSLENLMKIVR 129

Query: 128 FASESVDNRRLLCGIDRLLPVLVDLLCNVDAG-DLVDQTVRLLHLIRSEIGDKERFVKMM 187
           F  +S  NR  L      +P+LVD++       +LV   +R+L  I+    D+ER   +M
Sbjct: 130 FVKDSDSNREFLSKKMEFVPMLVDIIRTKKTKIELVIMAIRILDSIKV---DRERLSNLM 189

Query: 188 L-NTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLIN- 247
           L N   +C++++LL +++G +E KI S  +L++++ DA++K++IAE DG++ E++K I+ 
Sbjct: 190 LANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSISI 249

Query: 248 -SENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKV 307
              +D +LIE+ LS LI+IS  KR++ KL+    I  +  +L  +++ +++++TEK LK+
Sbjct: 250 TESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILL-TETLTNVAVTEKSLKL 309

Query: 308 LETASTVREGRTEI-GEDS-VCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA-E 367
           LET S+ REGR EI G+D+  CV  +V+K+LKVS  ATEHAVT LW +CY+FR++K   E
Sbjct: 310 LETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKTVEE 369

Query: 368 ALTKANGLTKILLLMQSNCSPPVRQMARDLLKIFRINSKSCLSSYDTKTTHIMP 408
            + ++NG+TK+L+++QSNCS  VRQMA+DL+K+ + NS S L++Y+TKTTHIMP
Sbjct: 370 TVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFNS-SALAAYETKTTHIMP 414

BLAST of PI0016064 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 149.4 bits (376), Expect = 6.1e-36
Identity = 111/387 (28.68%), Postives = 192/387 (49.61%), Query Frame = 0

Query: 8   ITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNG-NNTCPATMQVLQTKEFVPN 67
           I IP  F CPISL++MK PV + TG+TYDR SI+KWL  G  N+CP T Q +   +  PN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 68  HNLHRLIQIWS--DSLHQRLHSPVSDSSLSSDQVLRLI-NRMDDHQSHPDSLTRLVSFAS 127
           H L RLIQ W   ++ +     P     +   ++ +LI +    H++    L RL    S
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVS 129

Query: 128 ESVDNRRLL--CGIDRLLPVLVDLLCNVDAGDLVDQTVRLLHLIRSEIGDKERFVKMMLN 187
           E+  N+R L   G+   L  +V    + + G L D+ + LL+ + +     E  +K +LN
Sbjct: 130 ENATNKRCLEAAGVPEFLANIVS--NDSENGSLTDEALNLLYHLET----SETVLKNLLN 189

Query: 188 T--DRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQELLKLINSE 247
              D N + SL  I+++G  E ++ +  LL+ +   A+    +     +  E++++++  
Sbjct: 190 NKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDR 249

Query: 248 NDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEKVLKVLET 307
                 ++ +  L++I    R + K V+ GVI  + +LL +   +S     E  + VL+ 
Sbjct: 250 ISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDL 309

Query: 308 ASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAAEALTKAN 367
                EGR E       +A + +K+L+VS  A++ AV  L SV            + +  
Sbjct: 310 LCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLG 369

Query: 368 GLTKILLLMQSNCSPPVRQMARDLLKI 387
            + K+ L++Q +C    ++ A++LLK+
Sbjct: 370 VVAKLCLVLQVSCGGKTKEKAKELLKL 390

BLAST of PI0016064 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-32
Identity = 113/394 (28.68%), Postives = 200/394 (50.76%), Query Frame = 0

Query: 4   DDLCITIPSDFRCPISLDVMKSPVSLCTGVTYDRSSIQKWLDNGNNTCPATMQVLQTKEF 63
           +++ ITIPS F+CPIS ++MK PV + +G+TYDR +I+KW ++G  TCP T  VL + E 
Sbjct: 27  EEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQ 86

Query: 64  VPNHNLHRLIQIW-SDSLHQRLHS-PVSDSSLSSDQVLRLINRMD------DHQSHPDSL 123
           +PNH + R+IQ W   SL   +   P     ++S QV  +  R+       D+ +  + +
Sbjct: 87  IPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMV 146

Query: 124 TRLVSFASESVDNRRLL--CGIDRLLPVLVDLLC-NVDAGDLVDQTVRLLHLIRSEIGDK 183
           T++     ES  NR+ +   G   +L V  D    N +A  L+++TV +L  +   IG  
Sbjct: 147 TKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWM-LPIG-L 206

Query: 184 ERFVKMMLNTDRNCISSLLLILRKGTVELKICSANLLEYLAIDAEAKIVIAETDGLMQEL 243
           E   K+   T  +  + L+ +LR G          LLE       A   + + +G+ +  
Sbjct: 207 EGQSKL---TTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHA---LTKINGVQEAF 266

Query: 244 LKLINSENDTTLIESLLSCLISISMPKRIKIKLVQLGVIKSVTKLLSESKSSSSISMTEK 303
           +K IN   D+T + SL+S    I   +    + ++L ++    ++L +S++    S+ EK
Sbjct: 267 MKSIN--RDSTCVNSLISIHHMILTNQETVSRFLELDLVNITVEMLVDSEN----SVCEK 326

Query: 304 VLKVLETASTVREGRTEIGEDSVCVAAIVQKVLKVSNAATEHAVTTLWSVCYLFRDEKAA 363
            L VL      +EGR ++  + + +  +V+K+LK+S    +  V+ +W VC      +  
Sbjct: 327 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCKSGDGSEVE 386

Query: 364 EALTKANGLTKILLLMQSNCSPPVRQMARDLLKI 387
           EAL +     K+++++Q  C    ++   +LLK+
Sbjct: 387 EAL-RLGAFKKLVVMLQVGCGEGTKEKVTELLKM 403

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLF41.0e-12055.11U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2... [more]
Q9LXE31.9e-10651.33U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Q9LSA67.1e-9046.62U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1... [more]
Q84TG38.6e-3528.68E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9C8D11.5e-3128.68U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5A7SMT05.0e-21195.59RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B2G85.0e-21195.59RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485262 PE=4 ... [more]
A0A0A0KJ125.0e-20392.65RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G484010 PE... [more]
A0A6J1GDY48.0e-19387.62RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453282... [more]
A0A6J1IPD92.3e-19287.38RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478747 P... [more]
Match NameE-valueIdentityDescription
XP_008441024.11.0e-21095.59PREDICTED: U-box domain-containing protein 27 [Cucumis melo] >KAA0025541.1 U-box... [more]
XP_038883742.14.6e-20393.14U-box domain-containing protein 27 [Benincasa hispida][more]
XP_004148672.11.0e-20292.65U-box domain-containing protein 27 [Cucumis sativus] >KGN48372.1 hypothetical pr... [more]
KAG7034432.11.3e-19287.62U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022950098.11.6e-19287.62U-box domain-containing protein 27 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G64660.17.3e-12255.11CYS, MET, PRO, and GLY protein 2 [more]
AT5G09800.11.3e-10751.33ARM repeat superfamily protein [more]
AT3G18710.15.1e-9146.62plant U-box 29 [more]
AT2G35930.16.1e-3628.68plant U-box 23 [more]
AT1G66160.11.1e-3228.68CYS, MET, PRO, and GLY protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 13..76
e-value: 3.2E-26
score: 103.1
IPR003613U box domainPFAMPF04564U-boxcoord: 10..77
e-value: 2.6E-17
score: 62.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 9..83
score: 34.411537
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 160..397
e-value: 2.8E-18
score: 68.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 3..88
e-value: 1.2E-25
score: 91.3
NoneNo IPR availablePANTHERPTHR22849:SF20U-BOX DOMAIN-CONTAINING PROTEIN 27-RELATEDcoord: 4..408
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 4..408
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 14..56
e-value: 1.92472E-24
score: 92.6818
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 6..78
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 183..394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016064.1PI0016064.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity