PI0015458 (gene) Melon (PI 482460) v1

Overview
NamePI0015458
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr12: 2784291 .. 2785693 (+)
RNA-Seq ExpressionPI0015458
SyntenyPI0015458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTTTTCTTCAGTGTTCCATGTTCTCTCTTCTGCTTCTTCAACCTCATGGTACTCATTCATTCCATAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCAATAGCAGCCGCTCCTGACAATTCCAGTACGCGATTTTATATTCATGTAGCCCCCGGCACTTACCATGAAGCTATTCAAATTCCAAAGGTTAAGAAGTTCATTGCTTTGGTAGGCGATGATGCATTCACGACCATTATCGTAGACAATCGAAGTAATGGAACAGGATTCAAGACCAACAGCTCTGCAACCCTTAGTAAGCTTCTAATTTCGATATCTTTTTTAAATTATGGTCTCTGTCCATTGATATTCACACCAAACTTTTGAAGATCTTAAGGTTTCAAATTTTTTTTTTTTTTTTTTAGGTAACTTGAGATGTTCTTTACTTTAAAGCAGTGTTTTTTTAGTTATATTTTAAATAAATATACAATAAAAATAAAGAGAGTAATCAATTTTACCTTTTTTTTTTAAGTACAGTAATGGGGGTTATGGAATTTCAACCACAATTTTTTTTTAATTTTGAAGTAGAGTAAAGAGTATGACTAAAGGGTTTTCTAATCTACAAACTTGGACAAATGTATTGGTTTAAAGAATCATTATATATATTATTGAATTTTGTTTTACTAAGTTTATTTTTATTTGGTGCAGCCGTCAAAGGTGACAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACGGTCAGGCAGTAGCAGTCTTTGACAAAGCCGACCAGACTGCTTACTACAAGTGCAAGTTCTTGGGTTTTCAAGACACGTTATATGTCAACGGAAAGTACCAATTCTTTAAGGAGTCCGACATATATGGAAGCGTTGATTTCATCTTTGGGCATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATGATCCACAGTCTGTTAGTGGTTTTTCATTTCAAAATTGTACGGTCACAGTTTCACCTGAAATATCTTCAAATAAACGGAACGTCAAAGTCTTTCTTGGTCGTCCGTGGAGACAATACTCAAACGTTGTGTTTATGGAATCATTTCTTGATGATGTGGTGGCATCGGAAGGATGGATGGAGTGGAAAGGGATACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACCACGGTCCTGGGGCTGATGTTTCCAAAAGAGTTAATTGGACTGGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTTAATGGATCTGGATGGTTGCCTGAAACTGGGATTCCGTTTAGACGTGGACTTCTTTCCTAA

mRNA sequence

ATGGCGTTTTTCTTCAGTGTTCCATGTTCTCTCTTCTGCTTCTTCAACCTCATGGTACTCATTCATTCCATAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCAATAGCAGCCGCTCCTGACAATTCCAGTACGCGATTTTATATTCATGTAGCCCCCGGCACTTACCATGAAGCTATTCAAATTCCAAAGGTTAAGAAGTTCATTGCTTTGGTAGGCGATGATGCATTCACGACCATTATCGTAGACAATCGAAGTAATGGAACAGGATTCAAGACCAACAGCTCTGCAACCCTTACCGTCAAAGGTGACAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACGGTCAGGCAGTAGCAGTCTTTGACAAAGCCGACCAGACTGCTTACTACAAGTGCAAGTTCTTGGGTTTTCAAGACACGTTATATGTCAACGGAAAGTACCAATTCTTTAAGGAGTCCGACATATATGGAAGCGTTGATTTCATCTTTGGGCATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATGATCCACAGTCTGTTAGTGGTTTTTCATTTCAAAATTGTACGGTCACAGTTTCACCTGAAATATCTTCAAATAAACGGAACGTCAAAGTCTTTCTTGGTCGTCCGTGGAGACAATACTCAAACGTTGTGTTTATGGAATCATTTCTTGATGATGTGGTGGCATCGGAAGGATGGATGGAGTGGAAAGGGATACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACCACGGTCCTGGGGCTGATGTTTCCAAAAGAGTTAATTGGACTGGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTTAATGGATCTGGATGGTTGCCTGAAACTGGGATTCCGTTTAGACGTGGACTTCTTTCCTAA

Coding sequence (CDS)

ATGGCGTTTTTCTTCAGTGTTCCATGTTCTCTCTTCTGCTTCTTCAACCTCATGGTACTCATTCATTCCATAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCAATAGCAGCCGCTCCTGACAATTCCAGTACGCGATTTTATATTCATGTAGCCCCCGGCACTTACCATGAAGCTATTCAAATTCCAAAGGTTAAGAAGTTCATTGCTTTGGTAGGCGATGATGCATTCACGACCATTATCGTAGACAATCGAAGTAATGGAACAGGATTCAAGACCAACAGCTCTGCAACCCTTACCGTCAAAGGTGACAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACGGTCAGGCAGTAGCAGTCTTTGACAAAGCCGACCAGACTGCTTACTACAAGTGCAAGTTCTTGGGTTTTCAAGACACGTTATATGTCAACGGAAAGTACCAATTCTTTAAGGAGTCCGACATATATGGAAGCGTTGATTTCATCTTTGGGCATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATGATCCACAGTCTGTTAGTGGTTTTTCATTTCAAAATTGTACGGTCACAGTTTCACCTGAAATATCTTCAAATAAACGGAACGTCAAAGTCTTTCTTGGTCGTCCGTGGAGACAATACTCAAACGTTGTGTTTATGGAATCATTTCTTGATGATGTGGTGGCATCGGAAGGATGGATGGAGTGGAAAGGGATACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACCACGGTCCTGGGGCTGATGTTTCCAAAAGAGTTAATTGGACTGGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTTAATGGATCTGGATGGTTGCCTGAAACTGGGATTCCGTTTAGACGTGGACTTCTTTCCTAA

Protein sequence

MAFFFSVPCSLFCFFNLMVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS
Homology
BLAST of PI0015458 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.0e-81
Identity = 147/311 (47.27%), Postives = 207/311 (66.56%), Query Frame = 0

Query: 27  NLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIALVGDDAF 86
           N+ V+ DGSGN K I +A+AAA     +R+ I++  GTY+E I++ K+K  I  VGD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV-KLKN-IMFVGDGIG 261

Query: 87  TTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTAYY 146
            TII  ++S G G  T  SAT+ V GDNF+A  +T  N+AGP N QAVA+   +D + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 147 KCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPSN-SITVTAQ 206
           +C F G+QDTLYV+ + QF++E DIYG+VDFIFG+  V+ Q+CNI+AR P N + T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 207 SKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVASEG 266
            + DP   +G    NC VT + ++   + +VK FLGRPW+QYS  V++++FLD ++   G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 267 WMEWKG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESAL-RFTVDNFVNGSGWL 326
           WMEW G   +N L+Y E+ N GPG+  + RV W GYH+L   S + +FTV NF+ G+ WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 327 PETGIPFRRGL 335
           P T +PF  GL
Sbjct: 502 PATNVPFTSGL 510

BLAST of PI0015458 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 7.2e-80
Identity = 140/317 (44.16%), Postives = 212/317 (66.88%), Query Frame = 0

Query: 21  IHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIAL 80
           +   ++NL+V++DG+GNF  I+DA+ AAP+ S TRF I++  G Y E +++PK K  I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 81  VGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKA 140
           +GD    T+I  NRS   G+ T  + T+ VKG  ++A+ ++F NSAGP   QAVA    +
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 334

Query: 141 DQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS--N 200
           D +A+Y+C+F G+QDTLYV+   QF++E DIYG++DFIFG+  V+FQ+ ++YAR P+  +
Sbjct: 335 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 394

Query: 201 SITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLD 260
            I  TAQS++     +G S  NC +  +P++   K N K +LGRPWR+YS  V ++SF+D
Sbjct: 395 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 454

Query: 261 DVVASEGWMEW-KGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKES-ALRFTVDNF 320
           D++   GW+E  K   +  L+YGE+ N GPGA+++KRV W G+  ++ ++ A +FTV  F
Sbjct: 455 DLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPF 514

Query: 321 VNGSGWLPETGIPFRRG 334
           ++GS WL  TGIPF  G
Sbjct: 515 IDGSTWLNSTGIPFSLG 531

BLAST of PI0015458 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.0e-79
Identity = 139/313 (44.41%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 25  SFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIALVGDD 84
           S +L V+ DG+GNF  I++AI+ AP+ S+ R  I+V  G Y E I IP  K  I L+GD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 85  AFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTA 144
           +  T I  NRS G G+ T  SATL V G+ F+A  +   N+AGP+  QAVA+   AD  A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 145 YYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYAR--MPSNSITV 204
            Y+C   G+QDTLY +   QF++E DIYG++D+IFG+  V+FQ CNI ++  MP     +
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 205 TAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVA 264
           TAQS+D     +G S QNC++  S ++ ++   VK +LGRPWR++S  V MES++D+ + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 265 SEGWMEWK-GIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFVNGSG 324
             GW +W  G  ++ L+YGE+NN+GPG++  KRVNW G+H++  E A  FT   F+ G G
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDG 534

Query: 325 WLPETGIPFRRGL 335
           WL  T  P+  G+
Sbjct: 535 WLGSTSFPYDNGI 547

BLAST of PI0015458 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-78
Identity = 137/319 (42.95%), Postives = 208/319 (65.20%), Query Frame = 0

Query: 24  ISFNLTVSLDGSGNFKRISDAIAAAP---DNSSTRFYIHVAPGTYHEAIQIPKVKKFIAL 83
           +S  +TV+ +G+GNF  I++A+ +AP   D ++  F I+V  G Y E + I K K+++ +
Sbjct: 255 VSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMM 314

Query: 84  VGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKA 143
           +GD    T++  NR+   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   A
Sbjct: 315 IGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSA 374

Query: 144 DQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPSNS- 203
           D + +Y C F  +QDTLY +   QF++E DIYG+VDFIFG+  V+FQDCN+Y R P  + 
Sbjct: 375 DLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQ 434

Query: 204 -ITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLD 263
              +TAQ + DP   +G S  NCT+  + ++ S+   VK +LGRPW++YS  VFM+S++D
Sbjct: 435 FNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYID 494

Query: 264 DVVASEGWMEWKG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 323
           +VV   GW EW G   ++ L+Y E+NN G G+  + RV W GYH+++   A  FTV+NF+
Sbjct: 495 EVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFL 554

Query: 324 NGSGWLPETGIPFRRGLLS 337
            G GW+ ++G+P+  GLLS
Sbjct: 555 LGDGWMVQSGVPYISGLLS 573

BLAST of PI0015458 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-78
Identity = 141/308 (45.78%), Postives = 202/308 (65.58%), Query Frame = 0

Query: 35  SGNFKRISDAIAAAPDNSSTR---FYIHVAPGTYHEAIQIPKVKKFIALVGDDAFTTIIV 94
           S NF  I+DAIAAAP+N+      F I+   G Y E I +P  KK + L+GD    TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 95  DNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTAYYKCKFL 154
            N +   G+ T + ++  V G+ FMA  +TF N+AGP+  QAVA+ + A+ +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 155 GFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMP--SNSITVTAQSKDD 214
           G+QDTLYV+   QF++E DIYG+VDFIFG+   +FQ+CNIYAR P       +TA  + D
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 215 PQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWMEW 274
           P   +G S  NCT+  +P++++  ++   FLGRPW+ YS  VFM+S++ D+V   GW+EW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 275 KG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFVNGSGWLPETGI 334
            G I ++ ++YGE++N GPGA+ ++RV W GY+LL+   A+ FTV NF  G  WLP+T I
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 609

Query: 335 PFRRGLLS 337
           PF  GLLS
Sbjct: 610 PFYGGLLS 617

BLAST of PI0015458 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 298/336 (88.69%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MAFFFSVPCSLFCFFNLMVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHV 60
           M FFF VPCSLFCFFNLM+LIHS+SFNLTVSLDGSGNFKRISDAIAAAP+NS+TRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  APGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESL 120
           APGTYHE +QIP++KKFIALVGDD FTTII+DNRSNGTGFKTN+SATLTV+GDNFMAESL
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 TFENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFG 180
           TFENSAGPQN QAVAVFDKA+ TAYYKC+FL FQDTLYVNGK QFFKESDIYGSVDFI G
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 HGQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFL 240
           +GQVMFQDCNIYARMP NSITVTAQSK   +SVSGFSFQNCTVTVS EISSNK+NVKVFL
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGY 300
           GRPW+QYS VVFMESFLDDVVASEGW+EW G+PVNNLFYGEFNN GPGADVSKRVNWT Y
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HLLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           HLLDKESALRFTVDNFVNGS WLPETGIPFRRGL S
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLHS 336

BLAST of PI0015458 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.0e-169
Identity = 288/319 (90.28%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 18  MVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKF 77
           M+ +HSISFNLTVSLDGSGNFKRISDAI AAP+NS+TRFYIHVAPGTYHEAIQIPK KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVF 137
           IAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+GDNFMA+SLTFENSAGPQNGQAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS 197
           DKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGHG+VMFQDCNIYARMPS
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFL 257
           NSITVTAQSKD  +S+SGFSFQNCTVTVSPEISSNK+NVKVFLGRPWRQYSNVVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 317
           DDVVASEGWMEWKG+PVNNLFYGEFNN GPGADVSKRVNWTGYHLLDK+SA+ FTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 318 NGSGWLPETGIPFRRGLLS 337
           +GS WL ETGIPFRRGLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

BLAST of PI0015458 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.8e-166
Identity = 283/319 (88.71%), Postives = 305/319 (95.61%), Query Frame = 0

Query: 18  MVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKF 77
           M+ +HSISFNLTVSLDGSGNFKRISDAI AAP+NS+TRFYIHVAPGTYHEAIQIPK KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVF 137
           IAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLTFENSAGPQNGQAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS 197
           DKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGHGQVMFQDCNIYARMPS
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFL 257
           NSITVTAQSKD  +S+SGFSFQN TVTV PEISSNK+NVKVFLGR WRQYSNVVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 317
           DDVVASEGWMEWKG+P+NNLFYGEFNN+GPGADVSKRVNWTGYH+L+KESA RFTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFI 300

Query: 318 NGSGWLPETGIPFRRGLLS 337
           N S WL ETGIPFR GLL+
Sbjct: 301 NRSKWLHETGIPFRHGLLT 319

BLAST of PI0015458 vs. ExPASy TrEMBL
Match: A0A1S3CP85 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.2e-143
Identity = 244/275 (88.73%), Postives = 263/275 (95.64%), Query Frame = 0

Query: 62  PGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLT 121
           PGTYHEAIQIPK KKFIAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 122 FENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGH 181
           FENSAGPQNGQAVAVFDKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGH
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 182 GQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLG 241
           G+VMFQDCNIYARMPSNSITVTAQSKD  +S+SGFSFQN TVTV PEISSNK+NVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 242 RPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYH 301
           R WRQYSNVVFMESFLDDVVASEGWMEWKG+P+NNLFYGEFNN+GPGADVSKRVNWTGYH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 302 LLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+N S WL ETGIPFR GLL+
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

BLAST of PI0015458 vs. ExPASy TrEMBL
Match: A0A5A7UNS3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.8e-142
Identity = 244/275 (88.73%), Postives = 262/275 (95.27%), Query Frame = 0

Query: 62  PGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLT 121
           PGTYHEAIQIPK KKFIAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 122 FENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGH 181
           FENSAGPQNGQAVAVFDKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGH
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 182 GQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLG 241
           GQVMFQDCNIYARMPSNSITVTAQSKD  +S+SGFSFQN TVTV  EISSNK+NVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 242 RPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYH 301
           R WRQYSNVVFMESFLDDVVASEGWMEWKG+P+ NLFYGEFNN+GPGADVSKRVNWTGYH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 302 LLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+NGS WL ETGIPFR GLL+
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

BLAST of PI0015458 vs. NCBI nr
Match: XP_004152582.2 (pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [Cucumis sativus])

HSP 1 Score: 611.7 bits (1576), Expect = 3.8e-171
Identity = 298/336 (88.69%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MAFFFSVPCSLFCFFNLMVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHV 60
           M FFF VPCSLFCFFNLM+LIHS+SFNLTVSLDGSGNFKRISDAIAAAP+NS+TRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  APGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESL 120
           APGTYHE +QIP++KKFIALVGDD FTTII+DNRSNGTGFKTN+SATLTV+GDNFMAESL
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 TFENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFG 180
           TFENSAGPQN QAVAVFDKA+ TAYYKC+FL FQDTLYVNGK QFFKESDIYGSVDFI G
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 HGQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFL 240
           +GQVMFQDCNIYARMP NSITVTAQSK   +SVSGFSFQNCTVTVS EISSNK+NVKVFL
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGY 300
           GRPW+QYS VVFMESFLDDVVASEGW+EW G+PVNNLFYGEFNN GPGADVSKRVNWT Y
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HLLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           HLLDKESALRFTVDNFVNGS WLPETGIPFRRGL S
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLHS 336

BLAST of PI0015458 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 605.9 bits (1561), Expect = 2.1e-169
Identity = 288/319 (90.28%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 18  MVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKF 77
           M+ +HSISFNLTVSLDGSGNFKRISDAI AAP+NS+TRFYIHVAPGTYHEAIQIPK KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVF 137
           IAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+GDNFMA+SLTFENSAGPQNGQAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS 197
           DKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGHG+VMFQDCNIYARMPS
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFL 257
           NSITVTAQSKD  +S+SGFSFQNCTVTVSPEISSNK+NVKVFLGRPWRQYSNVVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 317
           DDVVASEGWMEWKG+PVNNLFYGEFNN GPGADVSKRVNWTGYHLLDK+SA+ FTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 318 NGSGWLPETGIPFRRGLLS 337
           +GS WL ETGIPFRRGLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

BLAST of PI0015458 vs. NCBI nr
Match: TYJ99208.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 593.2 bits (1528), Expect = 1.4e-165
Identity = 283/319 (88.71%), Postives = 305/319 (95.61%), Query Frame = 0

Query: 18  MVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKF 77
           M+ +HSISFNLTVSLDGSGNFKRISDAI AAP+NS+TRFYIHVAPGTYHEAIQIPK KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVF 137
           IAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLTFENSAGPQNGQAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS 197
           DKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGHGQVMFQDCNIYARMPS
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFL 257
           NSITVTAQSKD  +S+SGFSFQN TVTV PEISSNK+NVKVFLGR WRQYSNVVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 317
           DDVVASEGWMEWKG+P+NNLFYGEFNN+GPGADVSKRVNWTGYH+L+KESA RFTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFI 300

Query: 318 NGSGWLPETGIPFRRGLLS 337
           N S WL ETGIPFR GLL+
Sbjct: 301 NRSKWLHETGIPFRHGLLT 319

BLAST of PI0015458 vs. NCBI nr
Match: XP_008465596.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-142
Identity = 244/275 (88.73%), Postives = 263/275 (95.64%), Query Frame = 0

Query: 62  PGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLT 121
           PGTYHEAIQIPK KKFIAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 122 FENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGH 181
           FENSAGPQNGQAVAVFDKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGH
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 182 GQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLG 241
           G+VMFQDCNIYARMPSNSITVTAQSKD  +S+SGFSFQN TVTV PEISSNK+NVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 242 RPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYH 301
           R WRQYSNVVFMESFLDDVVASEGWMEWKG+P+NNLFYGEFNN+GPGADVSKRVNWTGYH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 302 LLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+N S WL ETGIPFR GLL+
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

BLAST of PI0015458 vs. NCBI nr
Match: KAA0055285.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 515.4 bits (1326), Expect = 3.7e-142
Identity = 244/275 (88.73%), Postives = 262/275 (95.27%), Query Frame = 0

Query: 62  PGTYHEAIQIPKVKKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLT 121
           PGTYHEAIQIPK KKFIAL+GDDAFTTII+DNRSNGTGFKTN+SATLTV+G+NFMA+SLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 122 FENSAGPQNGQAVAVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGH 181
           FENSAGPQNGQAVAVFDKAD TAYYKC+FLGFQDTLYVNGKYQFFKES+IYGSVDFIFGH
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 182 GQVMFQDCNIYARMPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLG 241
           GQVMFQDCNIYARMPSNSITVTAQSKD  +S+SGFSFQN TVTV  EISSNK+NVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 242 RPWRQYSNVVFMESFLDDVVASEGWMEWKGIPVNNLFYGEFNNHGPGADVSKRVNWTGYH 301
           R WRQYSNVVFMESFLDDVVASEGWMEWKG+P+ NLFYGEFNN+GPGADVSKRVNWTGYH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 302 LLDKESALRFTVDNFVNGSGWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+NGS WL ETGIPFR GLL+
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

BLAST of PI0015458 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.9 bits (764), Expect = 5.1e-81
Identity = 140/317 (44.16%), Postives = 212/317 (66.88%), Query Frame = 0

Query: 21  IHSISFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIAL 80
           +   ++NL+V++DG+GNF  I+DA+ AAP+ S TRF I++  G Y E +++PK K  I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 81  VGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKA 140
           +GD    T+I  NRS   G+ T  + T+ VKG  ++A+ ++F NSAGP   QAVA    +
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 334

Query: 141 DQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPS--N 200
           D +A+Y+C+F G+QDTLYV+   QF++E DIYG++DFIFG+  V+FQ+ ++YAR P+  +
Sbjct: 335 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 394

Query: 201 SITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLD 260
            I  TAQS++     +G S  NC +  +P++   K N K +LGRPWR+YS  V ++SF+D
Sbjct: 395 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 454

Query: 261 DVVASEGWMEW-KGIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKES-ALRFTVDNF 320
           D++   GW+E  K   +  L+YGE+ N GPGA+++KRV W G+  ++ ++ A +FTV  F
Sbjct: 455 DLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPF 514

Query: 321 VNGSGWLPETGIPFRRG 334
           ++GS WL  TGIPF  G
Sbjct: 515 IDGSTWLNSTGIPFSLG 531

BLAST of PI0015458 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 295.4 bits (755), Expect = 5.7e-80
Identity = 139/313 (44.41%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 25  SFNLTVSLDGSGNFKRISDAIAAAPDNSSTRFYIHVAPGTYHEAIQIPKVKKFIALVGDD 84
           S +L V+ DG+GNF  I++AI+ AP+ S+ R  I+V  G Y E I IP  K  I L+GD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 85  AFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTA 144
           +  T I  NRS G G+ T  SATL V G+ F+A  +   N+AGP+  QAVA+   AD  A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 145 YYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYAR--MPSNSITV 204
            Y+C   G+QDTLY +   QF++E DIYG++D+IFG+  V+FQ CNI ++  MP     +
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 205 TAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVA 264
           TAQS+D     +G S QNC++  S ++ ++   VK +LGRPWR++S  V MES++D+ + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 265 SEGWMEWK-GIPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFVNGSG 324
             GW +W  G  ++ L+YGE+NN+GPG++  KRVNW G+H++  E A  FT   F+ G G
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDG 534

Query: 325 WLPETGIPFRRGL 335
           WL  T  P+  G+
Sbjct: 535 WLGSTSFPYDNGI 547

BLAST of PI0015458 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 295.0 bits (754), Expect = 7.4e-80
Identity = 137/319 (42.95%), Postives = 208/319 (65.20%), Query Frame = 0

Query: 24  ISFNLTVSLDGSGNFKRISDAIAAAP---DNSSTRFYIHVAPGTYHEAIQIPKVKKFIAL 83
           +S  +TV+ +G+GNF  I++A+ +AP   D ++  F I+V  G Y E + I K K+++ +
Sbjct: 255 VSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMM 314

Query: 84  VGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKA 143
           +GD    T++  NR+   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   A
Sbjct: 315 IGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSA 374

Query: 144 DQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMPSNS- 203
           D + +Y C F  +QDTLY +   QF++E DIYG+VDFIFG+  V+FQDCN+Y R P  + 
Sbjct: 375 DLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQ 434

Query: 204 -ITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLD 263
              +TAQ + DP   +G S  NCT+  + ++ S+   VK +LGRPW++YS  VFM+S++D
Sbjct: 435 FNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYID 494

Query: 264 DVVASEGWMEWKG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFV 323
           +VV   GW EW G   ++ L+Y E+NN G G+  + RV W GYH+++   A  FTV+NF+
Sbjct: 495 EVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFL 554

Query: 324 NGSGWLPETGIPFRRGLLS 337
            G GW+ ++G+P+  GLLS
Sbjct: 555 LGDGWMVQSGVPYISGLLS 573

BLAST of PI0015458 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 294.3 bits (752), Expect = 1.3e-79
Identity = 143/325 (44.00%), Postives = 209/325 (64.31%), Query Frame = 0

Query: 18  MVLIHSISFNLTVSLDGSGNFKRISDAIAAAPDN---SSTRFYIHVAPGTYHEAIQIPKV 77
           MVLI  I   +TVS DG+GNF  I+ A+AAAP+N   S+  F I+V  G Y E I I K 
Sbjct: 258 MVLISDI---VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKN 317

Query: 78  KKFIALVGDDAFTTIIVDNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAV 137
           K+++ ++GD    T++  NRS   G+ T +SAT  V   NF+A ++TF N+AGP+  QAV
Sbjct: 318 KRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAV 377

Query: 138 AVFDKADQTAYYKCKFLGFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYAR 197
           A+   AD + +Y C F  +QDTLY +   QF++E D+YG+VDFIFG+  V+FQ+CN+Y R
Sbjct: 378 ALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPR 437

Query: 198 --MPSNSITVTAQSKDDPQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVF 257
             MP+    +TAQ + DP   +G S QNCT+  + ++ S+   VK +LGRPW++YS  V+
Sbjct: 438 KPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVY 497

Query: 258 MESFLDDVVASEGWMEWKG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRF 317
           M+S++D  V   GW EW G   ++ L+Y E+NN GPG++ + RV W GYH+++   A  F
Sbjct: 498 MQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANF 557

Query: 318 TVDNFVNGSGWLPETGIPFRRGLLS 337
           TV      + W+ +TG+P+  GL+S
Sbjct: 558 TVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of PI0015458 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 294.3 bits (752), Expect = 1.3e-79
Identity = 141/308 (45.78%), Postives = 202/308 (65.58%), Query Frame = 0

Query: 35  SGNFKRISDAIAAAPDNSSTR---FYIHVAPGTYHEAIQIPKVKKFIALVGDDAFTTIIV 94
           S NF  I+DAIAAAP+N+      F I+   G Y E I +P  KK + L+GD    TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 95  DNRSNGTGFKTNSSATLTVKGDNFMAESLTFENSAGPQNGQAVAVFDKADQTAYYKCKFL 154
            N +   G+ T + ++  V G+ FMA  +TF N+AGP+  QAVA+ + A+ +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 155 GFQDTLYVNGKYQFFKESDIYGSVDFIFGHGQVMFQDCNIYARMP--SNSITVTAQSKDD 214
           G+QDTLYV+   QF++E DIYG+VDFIFG+   +FQ+CNIYAR P       +TA  + D
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 215 PQSVSGFSFQNCTVTVSPEISSNKRNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWMEW 274
           P   +G S  NCT+  +P++++  ++   FLGRPW+ YS  VFM+S++ D+V   GW+EW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 275 KG-IPVNNLFYGEFNNHGPGADVSKRVNWTGYHLLDKESALRFTVDNFVNGSGWLPETGI 334
            G I ++ ++YGE++N GPGA+ ++RV W GY+LL+   A+ FTV NF  G  WLP+T I
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 609

Query: 335 PFRRGLLS 337
           PF  GLLS
Sbjct: 610 PFYGGLLS 617

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048875.0e-8147.27Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O814157.2e-8044.16Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O487118.0e-7944.41Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK71.0e-7842.95Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q94CB11.8e-7845.78Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0LR141.9e-17188.69Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1[more]
A0A5A7ULX71.0e-16990.28Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290... [more]
A0A5D3BM286.8e-16688.71Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418... [more]
A0A1S3CP856.2e-14388.73Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1[more]
A0A5A7UNS31.8e-14288.73Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280... [more]
Match NameE-valueIdentityDescription
XP_004152582.23.8e-17188.69pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [... [more]
KAA0055286.12.1e-16990.28putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
TYJ99208.11.4e-16588.71putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
XP_008465596.11.3e-14288.73PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis... [more]
KAA0055285.13.7e-14288.73putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G02300.15.1e-8144.16Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26440.15.7e-8044.41Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.17.4e-8042.95Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.11.3e-7944.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.3e-7945.78Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 22..334
e-value: 8.7E-115
score: 385.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 27..319
e-value: 1.4E-106
score: 355.6
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 24..331
NoneNo IPR availablePANTHERPTHR31707:SF149PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 24..331
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 171..180
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 24..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015458.1PI0015458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity