Homology
BLAST of PI0015339 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 5.4e-148
Identity = 267/499 (53.51%), Postives = 350/499 (70.14%), Query Frame = 0
Query: 6 FLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
FL ++L ISL L F R NLPP+P LP IGHLHL+ +H++L LS+KYG
Sbjct: 10 FLAVVLLSISLKL---FPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 69
Query: 66 VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEHWR 125
VFSL+ G+RLV++VSSP+A +ECFTKNDI+ ANRP GKY+ YN TTMV SPYGEHWR
Sbjct: 70 VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 129
Query: 126 NLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTFNIVM 185
NLRR++ +EIFS LN F IRE+EVK+LL L +E++ L +L+FN+ M
Sbjct: 130 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTM 189
Query: 186 RMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFNKR 245
RMV GK++ DV DE K F+ L+ ++ H G++NPGDF+P WID + K+
Sbjct: 190 RMVAGKRYF---GQDVDSDE--AKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKK 249
Query: 246 IMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDILIAG 305
+ KI + D +Q L+ E R ++ MI HLL LQ++ PEYY+DQIIKG+I +L+AG
Sbjct: 250 VSKISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAG 309
Query: 306 IDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISETLRL 365
DTSAVT++WA+S LLN+P LEKA+TEI++ +G R++ E DL L+YL IISET RL
Sbjct: 310 TDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRL 369
Query: 366 SPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNVVGF 425
PA P+LVPH +S+DCK++GYDVP+ TI+L+N WAIHRDP W+ + FKPERH V
Sbjct: 370 CPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH-GGVEL 429
Query: 426 ENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLTMPKA 485
E S KL+PFG+GRR+CPG +A RVVGLTL +L+QCFEWKR+ +DM EG GLTMPKA
Sbjct: 430 EPS-KLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKA 489
Query: 486 KPLVAKCRPCFIMKAVLGE 495
+PL A C+P I+ V+ E
Sbjct: 490 QPLEALCKPRNILHKVVSE 497
BLAST of PI0015339 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 516.9 bits (1330), Expect = 2.5e-145
Identity = 263/493 (53.35%), Postives = 349/493 (70.79%), Query Frame = 0
Query: 7 LYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH- 66
LY + SLI L++ + FL KP + NLPP+P LP IGHL LLK P+H+TL++ S+ H
Sbjct: 10 LYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 69
Query: 67 ----VFSLKFGSRLVVLVSSPS-AVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 126
V SL+ GSRLV +VSS A +ECF KND++LANRP + GK++ YNNT M+ +PY
Sbjct: 70 DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 129
Query: 127 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLT 186
G+HWRNLRR+ T+EIFST RLN F +R +EV+RL+ RL +EL+PML+DLT
Sbjct: 130 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 189
Query: 187 FNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPS 246
FN +MRM+ GK++ E DE KR ++LV + A+ S N D++P+ S
Sbjct: 190 FNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 249
Query: 247 GFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQD 306
+ R+ K+G TD+ +QGL+D+ R +++ G MI HLL LQK+D EYY+DQIIKG+I
Sbjct: 250 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 309
Query: 307 ILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIIS 366
++IAG +TSAVTL+WALS+LLN+P ++ KA+ EID+ +G +R++ EADLS L YL+ I+
Sbjct: 310 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 369
Query: 367 ETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHV 426
ETLRL PA PLLVPH ASEDCKI YD+PR T +L+N WAIHRDPN W++ SFKPER
Sbjct: 370 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE 429
Query: 427 NVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGL 484
E + KLL FGLGRRACPG +A R+VGL L SL+QCFEW+R+ N+ VDM+EG G
Sbjct: 430 KE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 489
BLAST of PI0015339 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 502.3 bits (1292), Expect = 6.4e-141
Identity = 254/493 (51.52%), Postives = 339/493 (68.76%), Query Frame = 0
Query: 6 FLYILLSLISLLLGYSFLFKPHR--LNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKY 65
+L +++SL L++ F R NLPP P CLP IG+LH LK P+H T TLSQKY
Sbjct: 5 YLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKY 64
Query: 66 GHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEH 125
G +FSL FGSRLVV+VSS + QECFTKNDI+LANRP +GKY+ YNNTT+ SPYG+H
Sbjct: 65 GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 124
Query: 126 WRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTFNI 185
WRNLRRI ++EI S+ RLNSF IR +E+ RL+++L +EL PM ++TFN
Sbjct: 125 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNT 184
Query: 186 VMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFN 245
+MRMV GK++ N+ + D + F+ ++ ++++ GG+ N GDF+ W D G
Sbjct: 185 IMRMVSGKRY--YGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLE 244
Query: 246 KRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDILI 305
KR+ KI +RTD +QGL+DE R + N MI HLL Q++ PEYY+DQIIKGL+ +L+
Sbjct: 245 KRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 304
Query: 306 AGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISETL 365
AG DTS+VT++WA+S+LLN+P I++KAK E+D+HIG +R V+E D+S L YLQ I+ ETL
Sbjct: 305 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 364
Query: 366 RLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNVV 425
RL A PLLVPH +SED + GY++P+NTI+++N W IHRDPNLW + + FKPER
Sbjct: 365 RLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPER----- 424
Query: 426 GFENS---YKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGL 484
FE KLL FGLGRRACPG ++ R GLTL L+QCFEWKR+ +DM E +G+
Sbjct: 425 -FEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGI 484
BLAST of PI0015339 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-139
Identity = 255/496 (51.41%), Postives = 345/496 (69.56%), Query Frame = 0
Query: 6 FLYILLSLISLLLGYSFLFK--PHRL-NLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQK 65
F Y LLSL S ++ L K RL NLPP P +P IG+LH LKHP+H+T TLSQ
Sbjct: 3 FYYSLLSL-SFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 62
Query: 66 YGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGE 125
YG +FSL FGSRLVV+VSSPS ECFTKNDIILANRP +GKY+ YN TT+ + YG+
Sbjct: 63 YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 122
Query: 126 HWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRLL-------------ELEPMLLDL 185
HWRNLRRI+T+++ S RLNSF G+R +E RL+++LL EL P L ++
Sbjct: 123 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 182
Query: 186 TFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDP 245
TFN +MRM+ GK++ ++ + + D K+F+E+++++M+ G+ N GDF+PL +D
Sbjct: 183 TFNAMMRMISGKRYYGDDGD--VSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDL 242
Query: 246 SGFNKRIMKIGRRTDEAVQGLVDEIR--NEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGL 305
KR +I +R++ ++GL++E R N +G MI HLL+L ++ PEYYSD +IKGL
Sbjct: 243 DNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGL 302
Query: 306 IQDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQG 365
IQ +L+AG DTSAVT++W +S LLN+P +L+KAK E+D+ IG+ ++V+E DLS L YLQ
Sbjct: 303 IQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQN 362
Query: 366 IISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPE 425
IISETLRL P PLL+PH +SEDC I ++VP++TI+L NVW IHRDP W +A SFKPE
Sbjct: 363 IISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPE 422
Query: 426 RHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREG 484
R E K++ FGLGRRACPG+++A R VG T+ L+QCFEW+R S +DM EG
Sbjct: 423 RFEKE---EEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEG 482
BLAST of PI0015339 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 484.6 bits (1246), Expect = 1.4e-135
Identity = 243/492 (49.39%), Postives = 339/492 (68.90%), Query Frame = 0
Query: 10 LLSLISLLLGYSFLFKPHRL--------NLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQ 69
+LSL+S + Y LF + NLPP P LP IG+LH LK P+H+T + LS+
Sbjct: 3 ILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSE 62
Query: 70 KYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYG 129
KYGHVFSL FGSRLVV+VSS S Q+CFTKND++LANRP SGKY+ YN TT+ + YG
Sbjct: 63 KYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122
Query: 130 EHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTF 189
EHWRNLRRI+ L++ S R+NSFSGIR +E +RL+ RL +EL L D+TF
Sbjct: 123 EHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTF 182
Query: 190 NIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSG 249
N +MRM+ GK++ E+ + D +F+++V++++ G+ N DF+PL ++D
Sbjct: 183 NNIMRMISGKRYYGEDCD--TSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 242
Query: 250 FNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDI 309
KR+ I +TD ++GL++E R +++ N MI HLL LQ + PEYY+DQIIKGL +
Sbjct: 243 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 302
Query: 310 LIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISE 369
L+AG D+SAVTL+W++S+LLN+P +L+K K E+D+H+GQ+R+V+E+DL L+YL+ +I+E
Sbjct: 303 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 362
Query: 370 TLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVN 429
TLRL PLL+PH S++C I GY VP++TIVLIN WAIHRDP LW A++FKPER
Sbjct: 363 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 422
Query: 430 VVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLT 484
E KL+ FG+GRRACPG +A R + +TLA L+QCF+WK ++ +D+ E +G T
Sbjct: 423 KGELE---KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFT 482
BLAST of PI0015339 vs. ExPASy TrEMBL
Match:
A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)
HSP 1 Score: 935.3 bits (2416), Expect = 1.1e-268
Identity = 460/513 (89.67%), Postives = 480/513 (93.57%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLNSGKYLAYNNTTMVVS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLR------------RLLELEPMLLD 180
GEHWRNLRRISTLEIFSTTRLNSFS REEEVKRLLR R++ELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWID 240
LTFNIVMRMVGGKKFC+E NN+VLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPL NWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
Query: 241 PSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLI 300
PSG+ KRIMKIGR+TDE +QGLVDEIRNEEDEGN MIQHLLRLQKTDPEYYSDQIIKGL+
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
Query: 301 QDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGI 360
QDIL+AGIDTSAVTLQWALSHLLNNPI+LEKAK EIDS+IGQERMVNEADLSSLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360
Query: 361 ISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPER 420
ISETLRLSPAGPLLVPHC+SEDCKI GYDVPRNTIVLIN WAIHRDPNLWE+ASSFKPER
Sbjct: 361 ISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPER 420
Query: 421 HVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGE 480
HVN GFENSYKLLPFG+GRRACPGMAMA RV+GLTLASLVQCFEWKRMS+LLVDMREGE
Sbjct: 421 HVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGE 480
Query: 481 GLTMPKAKPLVAKCRPCFIMKAVLGEKNGHIIV 502
GLTMPK +PLVAKCRP FIM+AVL EKNGHII+
Sbjct: 481 GLTMPKVEPLVAKCRPRFIMEAVLSEKNGHIII 513
BLAST of PI0015339 vs. ExPASy TrEMBL
Match:
A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)
HSP 1 Score: 911.4 bits (2354), Expect = 1.7e-261
Identity = 453/512 (88.48%), Postives = 469/512 (91.60%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSAVQECFTKNDIILANRPSLNSGKYL+YNNTTM VSPY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLL------------RRLLELEPMLLD 180
GEHWRNLRRI TLEIFSTTRLNSFS IREEEVKRLL R++ELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNN--DVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFCKENN +VLEDEGYCKR KELVTQIMAH GSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
Query: 241 IDPSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKG 300
IDPSG+NKRIMKIGRR DE +Q LVDEIRNEEDEGN MIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQ 360
LIQDILIAGIDTSAVTLQWALSHLLNNPI+L+KAK EIDS+IGQERMVNE DLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKP 420
GIISETLRLSP GPLLVPHCASEDCKI GYDVPRNTIVLIN WAIHRDPN+WE+A SFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
Query: 421 ERHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMRE 480
ERHVN VGFENSYKLLPFGLGRRACPGMAMA RVVGLTLASL+QCFEWK+MSNLLVDMRE
Sbjct: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
Query: 481 GEGLTMPKAKPLVAKCRPCFIMKAVLGEKNGH 499
GEGLTMPK + LVAKCRP FIMK VLGEKNGH
Sbjct: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGH 512
BLAST of PI0015339 vs. ExPASy TrEMBL
Match:
A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)
HSP 1 Score: 800.8 bits (2067), Expect = 3.2e-228
Identity = 398/494 (80.57%), Postives = 437/494 (88.46%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLNSGKYLAYNNTTMVVS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLR------------RLLELEPMLLD 180
G+HWRNLRRISTLEIFSTTRLNSFS REEEVKRLLR R++ELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWID 240
LTFNIVMRMVGGKKFC+E NN+VLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPL NWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
Query: 241 PSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLI 300
PSG+ K IMKIGR+TDE +QGLVDEIRNEEDEGN MIQHLLRLQKTDPEYYSDQIIKGL+
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
Query: 301 QDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGI 360
QDI++AGIDTS ++L+WALS LLNNP +L+KAK EID IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
Query: 361 ISETLRLSPAGPLLVPHCA-SEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPE 420
ISETLRL P PLLVPHCA E+CKI+ Y++PR+TIVLIN WAIHRD LWE+A+ FKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
Query: 421 RHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREG 480
RH +V+ E YKL+PFG+GRRACPG MA RVVGL LASL+Q FEW+R+ + L+DM EG
Sbjct: 421 RH-SVIESE-LYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 480
Query: 481 EGLTMPKAKPLVAK 482
+GLTMPKA+PL+AK
Sbjct: 481 KGLTMPKAQPLIAK 492
BLAST of PI0015339 vs. ExPASy TrEMBL
Match:
A0A1S3C323 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)
HSP 1 Score: 776.5 bits (2004), Expect = 6.5e-221
Identity = 378/509 (74.26%), Postives = 430/509 (84.48%), Query Frame = 0
Query: 7 LYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGHV 66
L I L+S++ + FL +P LNLPPTPLF LP IGHLHLLKHPVH+TLQ LSQKYGHV
Sbjct: 6 LSISFFLLSVVFAFKFLLRPRWLNLPPTPLFWLPLIGHLHLLKHPVHQTLQNLSQKYGHV 65
Query: 67 FSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEHWRN 126
FSL+FGSRLVV+VSSPSAVQECFTKNDIILANRP L SGKYL YN++++ +SPYGE WRN
Sbjct: 66 FSLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWRN 125
Query: 127 LRRISTLEIFSTTRLNSFSGIREEEVKRLLR--------------RLLELEPMLLDLTFN 186
LRRI +LE+FST RLN F GIRE+EVKRLLR R++E+ P+L DLT N
Sbjct: 126 LRRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLN 185
Query: 187 IVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGF 246
IVMRMV GK F +E++N +F+E+VTQIM H G++NPGDFIPLWNWIDP+GF
Sbjct: 186 IVMRMVSGKMFYEEDSN----------KFREIVTQIMEHAGASNPGDFIPLWNWIDPTGF 245
Query: 247 NKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDIL 306
K++ K+G+ +DE +Q L+D IRN+ D GN MI HLL LQ +PE+Y+D K +IQDIL
Sbjct: 246 MKKVKKLGKTSDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDIL 305
Query: 307 IAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISET 366
+AGIDTSAVTLQWALSHLLNNPI+LEKAK EIDS+IGQERMVNEADLSSLSYLQGIISET
Sbjct: 306 LAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISET 365
Query: 367 LRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNV 426
LRLSPAGPLLVPHC+SEDCKI GYDVPRNTIVLIN WAIHRDPNLWE+ASSFKPERHVN
Sbjct: 366 LRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNA 425
Query: 427 VGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLTM 486
GFENSYKLLPFG+GRRACPGMAMA RV+GLTLASLVQCFEWKRMS+LLVDMREGEGLTM
Sbjct: 426 AGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTM 485
Query: 487 PKAKPLVAKCRPCFIMKAVLGEKNGHIIV 502
PK +PLVAKCRPCFIM+AVL EKNGHII+
Sbjct: 486 PKVEPLVAKCRPCFIMEAVLSEKNGHIII 504
BLAST of PI0015339 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 726.1 bits (1873), Expect = 1.0e-205
Identity = 350/497 (70.42%), Postives = 420/497 (84.51%), Query Frame = 0
Query: 7 LYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGHV 66
L I LSL SL + + FL +P+R NLPP+P F LP IGHLHL+KHPVH+TL LS YGHV
Sbjct: 86 LSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHV 145
Query: 67 FSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEHWRN 126
FSL++GSRLVV+VSSPSAVQECFTKNDI+LANRP L SGK+LAYN+TT+ VSPYGEHWRN
Sbjct: 146 FSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRN 205
Query: 127 LRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTFNIVMR 186
LRRISTLEIFST+RLN F GIRE+EVK LLR+L +EL+ MLLD TFNIV+R
Sbjct: 206 LRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVR 265
Query: 187 MVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFNKRI 246
M+ GKK+ E+ L DEG K+F+E+V +I+A+GG+ N GDF+PLWNWIDP+G+ KRI
Sbjct: 266 MIAGKKYYGED----LRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRI 325
Query: 247 MKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDILIAGI 306
MKIG R+DE +Q LVDEIRN++D GN MI HLL LQ T PEY+SDQIIKGL Q +L+AGI
Sbjct: 326 MKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGI 385
Query: 307 DTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISETLRLS 366
DT+A+TL+WALS LLNNP +L+KA+ EID IG++R+V+E DLSSLSYLQG+I+ETLRL+
Sbjct: 386 DTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLN 445
Query: 367 PAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNVVGFE 426
PA PLLVPHCASEDCKIEGYD+PR+TI+LIN WAIHRD N+WE+A+SFKPERH N +G
Sbjct: 446 PAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGV 505
Query: 427 NSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLTMPKAK 486
+SYKLLPFGLGRRACPGM MA RVVGLTLASL+QCFEW+R+++ LVDM EGEGLTMPKA+
Sbjct: 506 DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQ 565
Query: 487 PLVAKCRPCFIMKAVLG 494
PLVAKC+P I+KA+LG
Sbjct: 566 PLVAKCKPRPILKAILG 578
BLAST of PI0015339 vs. NCBI nr
Match:
KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 935.3 bits (2416), Expect = 2.3e-268
Identity = 460/513 (89.67%), Postives = 480/513 (93.57%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLNSGKYLAYNNTTMVVS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLR------------RLLELEPMLLD 180
GEHWRNLRRISTLEIFSTTRLNSFS REEEVKRLLR R++ELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWID 240
LTFNIVMRMVGGKKFC+E NN+VLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPL NWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
Query: 241 PSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLI 300
PSG+ KRIMKIGR+TDE +QGLVDEIRNEEDEGN MIQHLLRLQKTDPEYYSDQIIKGL+
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
Query: 301 QDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGI 360
QDIL+AGIDTSAVTLQWALSHLLNNPI+LEKAK EIDS+IGQERMVNEADLSSLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360
Query: 361 ISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPER 420
ISETLRLSPAGPLLVPHC+SEDCKI GYDVPRNTIVLIN WAIHRDPNLWE+ASSFKPER
Sbjct: 361 ISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPER 420
Query: 421 HVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGE 480
HVN GFENSYKLLPFG+GRRACPGMAMA RV+GLTLASLVQCFEWKRMS+LLVDMREGE
Sbjct: 421 HVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGE 480
Query: 481 GLTMPKAKPLVAKCRPCFIMKAVLGEKNGHIIV 502
GLTMPK +PLVAKCRP FIM+AVL EKNGHII+
Sbjct: 481 GLTMPKVEPLVAKCRPRFIMEAVLSEKNGHIII 513
BLAST of PI0015339 vs. NCBI nr
Match:
XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])
HSP 1 Score: 911.4 bits (2354), Expect = 3.5e-261
Identity = 453/512 (88.48%), Postives = 469/512 (91.60%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKTLQTLS
Sbjct: 1 MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLV+LVSSPSAVQECFTKNDIILANRPSLNSGKYL+YNNTTM VSPY
Sbjct: 61 QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLL------------RRLLELEPMLLD 180
GEHWRNLRRI TLEIFSTTRLNSFS IREEEVKRLL R++ELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNN--DVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNW 240
LT NIVMRMVGGKKFCKENN +VLEDEGYCKR KELVTQIMAH GSTNPGDFIPLWNW
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240
Query: 241 IDPSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKG 300
IDPSG+NKRIMKIGRR DE +Q LVDEIRNEEDEGN MIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQ 360
LIQDILIAGIDTSAVTLQWALSHLLNNPI+L+KAK EIDS+IGQERMVNE DLSSLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360
Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKP 420
GIISETLRLSP GPLLVPHCASEDCKI GYDVPRNTIVLIN WAIHRDPN+WE+A SFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKP 420
Query: 421 ERHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMRE 480
ERHVN VGFENSYKLLPFGLGRRACPGMAMA RVVGLTLASL+QCFEWK+MSNLLVDMRE
Sbjct: 421 ERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMRE 480
Query: 481 GEGLTMPKAKPLVAKCRPCFIMKAVLGEKNGH 499
GEGLTMPK + LVAKCRP FIMK VLGEKNGH
Sbjct: 481 GEGLTMPKVESLVAKCRPRFIMKVVLGEKNGH 512
BLAST of PI0015339 vs. NCBI nr
Match:
XP_038902241.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-239
Identity = 412/512 (80.47%), Postives = 446/512 (87.11%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFD L ILLSL+ LLLGYSFL KPH LNLPPTPLFCLPFIGHLHLLKHPVHK Q LS
Sbjct: 1 MDFDLLLCILLSLVYLLLGYSFLLKPHGLNLPPTPLFCLPFIGHLHLLKHPVHKIFQNLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGS LVVLVSSPSAVQECFTKNDIILANRPSLN GKYLAYNNT+M +SPY
Sbjct: 61 QKYGHVFSLKFGSHLVVLVSSPSAVQECFTKNDIILANRPSLNPGKYLAYNNTSMALSPY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLR------------RLLELEPMLLD 180
GEHWRNLRRISTLEIFS TR N F RE+EVKRLL R++ELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSATRFNLFLKSREDEVKRLLYKLCGNDTLEDEFRVVELEPMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWID 240
LTFNIVMRMVGGKKFC+ENN +V EDEGY KRF+ELVTQIMAHGGSTNPGDFIPLWNWID
Sbjct: 181 LTFNIVMRMVGGKKFCEENNKNVSEDEGYSKRFRELVTQIMAHGGSTNPGDFIPLWNWID 240
Query: 241 PSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLI 300
P+G+NKR+MK+GRR+DE +QGL+DEIRN+EDEG MIQHLLRLQ TDPEYYSDQ+IKGL+
Sbjct: 241 PTGYNKRLMKLGRRSDEVLQGLIDEIRNQEDEGINMIQHLLRLQNTDPEYYSDQVIKGLV 300
Query: 301 QDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGI 360
QDIL+ GIDTSAVTL+WALSHLLNNPI+LE+AK EID IGQERMVNEADLSSLSYLQGI
Sbjct: 301 QDILLGGIDTSAVTLEWALSHLLNNPIVLERAKAEIDCFIGQERMVNEADLSSLSYLQGI 360
Query: 361 ISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPER 420
ISETLRLSP GPLL PH AS+DC I GYDVPRNT+VL N+WAIHRDPNLWE+A+SFKPER
Sbjct: 361 ISETLRLSPPGPLL-PHYASKDCTIGGYDVPRNTMVLFNIWAIHRDPNLWEDATSFKPER 420
Query: 421 HVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGE 480
H NV E SYKLLPFGLGRRACPGMA+A RV+GLTLASL+QCFEWKR S LVDM EG+
Sbjct: 421 HANVAEVEYSYKLLPFGLGRRACPGMAIAQRVIGLTLASLIQCFEWKRTSKSLVDMSEGK 480
Query: 481 GLTMPKAKPLVAKCRPCFIMKAVLGEKNGHII 501
GLTMPKAKPL+ K RPC IMKA+L E II
Sbjct: 481 GLTMPKAKPLIVKYRPCLIMKAILSETTDQII 511
BLAST of PI0015339 vs. NCBI nr
Match:
XP_038902403.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 834.3 bits (2154), Expect = 5.4e-238
Identity = 411/508 (80.91%), Postives = 446/508 (87.80%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFD ILLSL+ LLLGY+ L KP+RLNLPPTPLFCLPFIGHLHLLKHPVH+T Q LS
Sbjct: 1 MDFDILFCILLSLVCLLLGYTSLLKPNRLNLPPTPLFCLPFIGHLHLLKHPVHQTFQNLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRL+VLVSSPS VQECFTKNDIILANRPSLN+GKYLAYNN TM VS Y
Sbjct: 61 QKYGHVFSLKFGSRLIVLVSSPSVVQECFTKNDIILANRPSLNTGKYLAYNNITMAVSSY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL-------------LELEPMLL 180
GEHWRNLRRISTLEIFS TR+NSF RE+EVKRLL +L ++L+PMLL
Sbjct: 121 GEHWRNLRRISTLEIFSMTRVNSFLRTREDEVKRLLCKLCGNCYTLEDKFRAVDLKPMLL 180
Query: 181 DLTFNIVMRMVGGKKFCKE-NNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNW 240
DLTFNIVMRMVGGKKFC+E NNN+VLEDEGY KRF+E+VTQIMAH GSTNP DFIPLWNW
Sbjct: 181 DLTFNIVMRMVGGKKFCEENNNNNVLEDEGYSKRFREVVTQIMAHSGSTNPEDFIPLWNW 240
Query: 241 IDPSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKG 300
IDP+GFNKRIMK+GRR DE +QGLVDEIRN+EDEGN MI HLLRLQ TD EYYSDQ+IKG
Sbjct: 241 IDPTGFNKRIMKLGRRADEILQGLVDEIRNQEDEGNNMIHHLLRLQNTDLEYYSDQVIKG 300
Query: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQ 360
LI DILIAGIDT+AVTL+WALSHLLNNPI+LE+AK EID IGQERMVNEADLSSLSYLQ
Sbjct: 301 LIHDILIAGIDTTAVTLEWALSHLLNNPIVLERAKAEIDCSIGQERMVNEADLSSLSYLQ 360
Query: 361 GIISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKP 420
GIISETLRLSP GPLLVPHCASEDCKI GYD+PR TIVLINVWAIHRDPNLWE+A+SFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDIPRKTIVLINVWAIHRDPNLWEDATSFKP 420
Query: 421 ERHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMRE 480
ERH NV E SYKLLPFGLGRRACPGMAMA R++GLTLASL+QCFEWKR S LVDM E
Sbjct: 421 ERHANVAEVEYSYKLLPFGLGRRACPGMAMAQRIIGLTLASLIQCFEWKRTSKSLVDMSE 480
Query: 481 GEGLTMPKAKPLVAKCRPCFIMKAVLGE 495
G+GLTMPK +P++AKCRP IMKAVL E
Sbjct: 481 GKGLTMPKVEPIMAKCRPRLIMKAVLSE 508
BLAST of PI0015339 vs. NCBI nr
Match:
XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])
HSP 1 Score: 800.8 bits (2067), Expect = 6.6e-228
Identity = 398/494 (80.57%), Postives = 437/494 (88.46%), Query Frame = 0
Query: 1 MDFDHFLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLS 60
MDFDH LY LLSLISLLLGYSFLFKPHRLNLPPTPLF LPFIGHLHLLKHPVHKT QTLS
Sbjct: 1 MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
Query: 61 QKYGHVFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 120
QKYGHVFSLKFGSRLVVLVSSPSA+QECFTKNDIILANRPSLNSGKYLAYNNTTMVVS Y
Sbjct: 61 QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLR------------RLLELEPMLLD 180
G+HWRNLRRISTLEIFSTTRLNSFS REEEVKRLLR R++ELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
Query: 181 LTFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWID 240
LTFNIVMRMVGGKKFC+E NN+VLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPL NWID
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240
Query: 241 PSGFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLI 300
PSG+ K IMKIGR+TDE +QGLVDEIRNEEDEGN MIQHLLRLQKTDPEYYSDQIIKGL+
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
Query: 301 QDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGI 360
QDI++AGIDTS ++L+WALS LLNNP +L+KAK EID IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
Query: 361 ISETLRLSPAGPLLVPHCA-SEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPE 420
ISETLRL P PLLVPHCA E+CKI+ Y++PR+TIVLIN WAIHRD LWE+A+ FKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
Query: 421 RHVNVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREG 480
RH +V+ E YKL+PFG+GRRACPG MA RVVGL LASL+Q FEW+R+ + L+DM EG
Sbjct: 421 RH-SVIESE-LYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 480
Query: 481 EGLTMPKAKPLVAK 482
+GLTMPKA+PL+AK
Sbjct: 481 KGLTMPKAQPLIAK 492
BLAST of PI0015339 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 516.9 bits (1330), Expect = 1.8e-146
Identity = 263/493 (53.35%), Postives = 349/493 (70.79%), Query Frame = 0
Query: 7 LYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH- 66
LY + SLI L++ + FL KP + NLPP+P LP IGHL LLK P+H+TL++ S+ H
Sbjct: 10 LYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 69
Query: 67 ----VFSLKFGSRLVVLVSSPS-AVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPY 126
V SL+ GSRLV +VSS A +ECF KND++LANRP + GK++ YNNT M+ +PY
Sbjct: 70 DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 129
Query: 127 GEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLT 186
G+HWRNLRR+ T+EIFST RLN F +R +EV+RL+ RL +EL+PML+DLT
Sbjct: 130 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 189
Query: 187 FNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPS 246
FN +MRM+ GK++ E DE KR ++LV + A+ S N D++P+ S
Sbjct: 190 FNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 249
Query: 247 GFNKRIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQD 306
+ R+ K+G TD+ +QGL+D+ R +++ G MI HLL LQK+D EYY+DQIIKG+I
Sbjct: 250 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 309
Query: 307 ILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIIS 366
++IAG +TSAVTL+WALS+LLN+P ++ KA+ EID+ +G +R++ EADLS L YL+ I+
Sbjct: 310 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 369
Query: 367 ETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHV 426
ETLRL PA PLLVPH ASEDCKI YD+PR T +L+N WAIHRDPN W++ SFKPER
Sbjct: 370 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFE 429
Query: 427 NVVGFENSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGL 484
E + KLL FGLGRRACPG +A R+VGL L SL+QCFEW+R+ N+ VDM+EG G
Sbjct: 430 KE---EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 489
BLAST of PI0015339 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 499.2 bits (1284), Expect = 3.8e-141
Identity = 254/492 (51.63%), Postives = 354/492 (71.95%), Query Frame = 0
Query: 7 LYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH- 66
L+++LSLI L+ KP NLPP+P + LP IGHL LLK P+H+T +LSQ +
Sbjct: 11 LFVVLSLIYLI--GKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 70
Query: 67 -VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEHW 126
+FSL+ G+RLV + SS S +ECFTKND++LANRP+ K++AY+ TTM+ + YG+HW
Sbjct: 71 PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 130
Query: 127 RNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTFNIV 186
RNLRRI ++EIFS RLNSF IR++E++RL+ RL ++++ ML DLTFN +
Sbjct: 131 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 190
Query: 187 MRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFNK 246
+RMV GK++ D +ED+ KR ++L+ ++A G+ N D++P+ + S +
Sbjct: 191 LRMVAGKRY----YGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYET 250
Query: 247 RIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDILIA 306
R+ K+ R DE +QGLVDE R +++GN MI HLL LQ++ P+Y++D+IIKG + +++A
Sbjct: 251 RVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILA 310
Query: 307 GIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISETLR 366
G DTSAVTL+WALS++LN+P +L KA+ EID IG +R+++E+D+S+L YLQ I+SETLR
Sbjct: 311 GTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLR 370
Query: 367 LSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNVVG 426
L PA P+L+PH ASEDCK+ GYD+PR TI+L NVWAIHRDP LW++ SFKPER
Sbjct: 371 LYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPER------ 430
Query: 427 FE---NSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLT 484
FE + KL+PFGLGRRACPG +AHR++ LTL SL+QC EW+++ VDM EG+G+T
Sbjct: 431 FEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE-EVDMSEGKGVT 484
BLAST of PI0015339 vs. TAIR 10
Match:
AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )
HSP 1 Score: 488.8 bits (1257), Expect = 5.2e-138
Identity = 252/501 (50.30%), Postives = 345/501 (68.86%), Query Frame = 0
Query: 4 DHFLYILLSLISLLLGYSFLF--KPHRLNLPPTPLFCLPFIGHLHLLKHP-VHKTLQTLS 63
D L IL +L+++ + L KP++ NLPP+P C P IGHLHLLK P +H+TL LS
Sbjct: 2 DCILLILTTLVAIFIVKIVLLVTKPNK-NLPPSPNICFPIIGHLHLLKKPLLHRTLSHLS 61
Query: 64 QKYGHVFSLKFGSRLVVLVSSPSAVQECF-TKNDIILANRPSLNSGKYLAYNNTTMVVSP 123
G VFSL+ GSRL V++SSP+A +ECF TKNDI+LANRP GKY+AY+ T+MV +P
Sbjct: 62 HSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAP 121
Query: 124 YGEHWRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDL 183
YG+HWRNLRRI+ LE+FST RLN+ + IR +EVK LL++L +EL +L L
Sbjct: 122 YGDHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGL 181
Query: 184 TFNIVMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDP 243
T N++MRM+ GK+F +E++ G F+ELV +I+ + NP DF+P W D
Sbjct: 182 TLNVIMRMMTGKRFFEEDDGG---KAGISLEFRELVAEILELSAADNPADFLPALRWFDY 241
Query: 244 SGFNKRIMKIGRRTDEAVQGLVDEIRNEEDE---GNAMIQHLLRLQKTDPEYYSDQIIKG 303
G KR +IG R D +QG +DE R +D N MI HLL Q+ +P YSDQ IKG
Sbjct: 242 KGLVKRAKRIGERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKG 301
Query: 304 LIQDILIAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHI---GQERMVNEADLSSLS 363
LI +++ G DTSA+T++WA+S+LLN+P ILE + ID+ + R++ E DL +++
Sbjct: 302 LILMMVVGGTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMN 361
Query: 364 YLQGIISETLRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASS 423
YL+ ++SETLRL P PL+VPH S DC I G++VPR+TIVL+N+WAIHRDP++W++ +S
Sbjct: 362 YLKNVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTS 421
Query: 424 FKPERHVNVVGFEN-SYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLV 483
FKPER F + + K++PFGLGRRACPG+++A+RVVGL L S++QCFEW+ S V
Sbjct: 422 FKPERFEGSDQFGHYNGKMMPFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQV 481
BLAST of PI0015339 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 488.0 bits (1255), Expect = 8.9e-138
Identity = 257/494 (52.02%), Postives = 338/494 (68.42%), Query Frame = 0
Query: 6 FLYILLSLISLLLGYSFLFKPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH 65
F +I+LSLI L+ +LNLPP+P + LP IGHL LLK P+H+ ++SQ G
Sbjct: 10 FCFIVLSLIFLIGRIK-----RKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGD 69
Query: 66 --VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEH 125
+ SL+ G+RL+ +VSS S +ECFTKND+ILANR + S K+++Y N+T+V + Y EH
Sbjct: 70 APIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEH 129
Query: 126 WRNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRLL----------ELEPMLLDLTFNI 185
WRNLRRI LEIFS RLNSFS IR +E++RL+ RLL E++ M DLTFN
Sbjct: 130 WRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNN 189
Query: 186 VMRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFN 245
++RM+ GK + D ED+ KR + L+ + M+ G N D+IP+ WI S
Sbjct: 190 IIRMLAGKCY----YGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--E 249
Query: 246 KRIMKIGRRTDEAVQGLVDEIR-NEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDIL 305
RI K+ R DE +QGLVDE R +E + N M+ HLL LQ+T PEYY D+IIKG + ++
Sbjct: 250 TRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLI 309
Query: 306 IAGIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISET 365
G DT+AVTL+WALS LLNNP +L KA+ EID IG +R++ E+D+ +L YLQ I+SET
Sbjct: 310 AGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSET 369
Query: 366 LRLSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNV 425
LRL PA P+L+PH AS+DCK+ GYD+PR T++L N WAIHRDP LW++ +SFKPER
Sbjct: 370 LRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPER---- 429
Query: 426 VGFE---NSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEG 484
FE + KL+PFGLGRRACPG +A R+V L+L SL+QCFEW+R+ VDM EG G
Sbjct: 430 --FEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPG 486
BLAST of PI0015339 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 486.1 bits (1250), Expect = 3.4e-137
Identity = 244/503 (48.51%), Postives = 344/503 (68.39%), Query Frame = 0
Query: 9 ILLSLISLLLGYSFLF--KPHRLNLPPTPLFCLPFIGHLHLLKHPVHKTLQTLSQKYGH- 68
+LLS + L + L + NLPP+P + LP IGHLHLLK PVH+T ++S+ G+
Sbjct: 7 LLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 66
Query: 69 -VFSLKFGSRLVVLVSSPSAVQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSPYGEHW 128
+F L+ G+RLV ++SS S +ECFTKND++LANRP + K++ YN T M+ + YG+HW
Sbjct: 67 PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 126
Query: 129 RNLRRISTLEIFSTTRLNSFSGIREEEVKRLLRRL----------LELEPMLLDLTFNIV 188
RNLRRI+ +EIFS+ R+++FS IR++E++RL+ L +EL+ +L +L FN +
Sbjct: 127 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 186
Query: 189 MRMVGGKKFCKENNNDVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLWNWIDPSGFNK 248
+ MV GK++ ED K +EL+ +IMA GS N D++P NW+ + F
Sbjct: 187 IMMVAGKRYYGTGT----EDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFEN 246
Query: 249 RIMKIGRRTDEAVQGLVDEIRNEEDEGNAMIQHLLRLQKTDPEYYSDQIIKGLIQDILIA 308
+ +G R D +Q LVDE R E+++G +I HLL Q+T+PEYY+D IIKG+I +++A
Sbjct: 247 QTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLA 306
Query: 309 GIDTSAVTLQWALSHLLNNPIILEKAKTEIDSHIGQERMVNEADLSSLSYLQGIISETLR 368
G DTS+VTL+WA+S+LLN+P ILEKA+ EID IG +R+V E+D+ +L YLQ I+SETLR
Sbjct: 307 GTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLR 366
Query: 369 LSPAGPLLVPHCASEDCKIEGYDVPRNTIVLINVWAIHRDPNLWENASSFKPERHVNVVG 428
L PA PLL+PH +S++CK+ GYD+PR T++L NVWA+HRDP LWE FKPER
Sbjct: 367 LYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPER------ 426
Query: 429 FE---NSYKLLPFGLGRRACPGMAMAHRVVGLTLASLVQCFEWKRMSNLLVDMREGEGLT 488
FE + KL+PFG+GRRACPG + R+V L L L+Q FEW+R+ LVDM EGEG+T
Sbjct: 427 FEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGIT 486
Query: 489 MPKAKPLVAKCRPCFIMKAVLGE 495
MPKA PL A C+ +A++G+
Sbjct: 487 MPKATPLRAMCK----ARAIVGK 493
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 5.4e-148 | 53.51 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q9FG65 | 2.5e-145 | 53.35 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Q6WNQ8 | 6.4e-141 | 51.52 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNQ9 | 1.2e-139 | 51.41 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
P93147 | 1.4e-135 | 49.39 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SPU0 | 1.1e-268 | 89.67 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... | [more] |
A0A0A0LQI5 | 1.7e-261 | 88.48 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1 | [more] |
A0A1S3C1S2 | 3.2e-228 | 80.57 | LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L... | [more] |
A0A1S3C323 | 6.5e-221 | 74.26 | cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1 | [more] |
A0A6J1D5S6 | 1.0e-205 | 70.42 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
Match Name | E-value | Identity | Description | |
KAA0032538.1 | 2.3e-268 | 89.67 | isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa] | [more] |
XP_004151924.2 | 3.5e-261 | 88.48 | cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022... | [more] |
XP_038902241.1 | 1.7e-239 | 80.47 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_038902403.1 | 5.4e-238 | 80.91 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_008455822.2 | 6.6e-228 | 80.57 | PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo] | [more] |
Match Name | E-value | Identity | Description | |
AT5G36220.1 | 1.8e-146 | 53.35 | cytochrome p450 81d1 | [more] |
AT4G37370.1 | 3.8e-141 | 51.63 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37310.1 | 5.2e-138 | 50.30 | cytochrome P450, family 81, subfamily H, polypeptide 1 | [more] |
AT4G37360.1 | 8.9e-138 | 52.02 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37320.1 | 3.4e-137 | 48.51 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |