PI0015309 (gene) Melon (PI 482460) v1

Overview


Name	PI0015309
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	kinetochore protein NDC80 homolog
Location	chr03: 5861516 .. 5864750 (-)
RNA-Seq Expression	PI0015309
Synteny	PI0015309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCCAACTCGCCGCCGCCGTCCAAATGGGTCTTTCAATCCTCCACCGCCGTCTACTTCCCAATTCCGCCGCGATTCAGATGTCAACTTTTCCAGTAGTCGCCCTTCTTCCATCAGAATGGCTCGTCCCGCCGCCTCCGAGCCTTTCAAATTTCTGCTATCAACTCCATCAATTCGTCTCTCAAGGAGCTCTTCTCTCACTCCTTCAACGTAACATTCAAACTCTTCGCTCCTCCTTCTCTCAAAGATCTCACTGAAACCATGCATTTGCTTTTTCAATGTCTCGATTATCGCTTGACTAAGCTTGAGGACGATCTCCCCGTTGTCCTCAAATGCCTTTGGTATCCCTTCAAAATTAATAAAGGTATCCTCAAGAACCCCCCTGCCCCTCATCAGTGGCACCAAATACTTGCCTAATTCACTGGCTTGTGCAGGTCGCTGTTTATAACTATAACCTTGCAAACGATTCGGATTCGTTTTTTACGTGCAACGAAGTGAATATATATGTGCTGAATAGCTATGTGCAATATATTCGAGGGGATGATGATTCGGTGGAGGCTTTGGATCAGGAGTTTATGGAGCGGTTGAAGAAAGGGAGGGATGAGGCTGTTGAAAGCTTGAAGGAATTGGAGAAGGAAGCTAAGGAGCTTGAAGCCAAAGTCGAGGCCTTGCGGTCGAATCCCTCACAGAGGGAGGTTCTTGACAAGCAGCAGAGTCTACTTGAAGAGGATGTGAAGAAGTTTCATACAATGACCGCTGGGTACAGTGAGAAGATACGGAAATGAAGAAGATTTTGGAGGAGAAGGAAAAGGAGTTGGAAGTTAAAGTACGTGAGAACAAGAAGATTGGTGAAGAGAATGAAGAACTGAAGAAGAGGGTGGAGTTTCAGATGTTTAATACTAGAGAGGTGGAAAGGATGTGGAGAGAATTGCATGCTGTGGAGAGGGATATCGGTGATGCTGAAGTCTCAAGGATTGCGTGGGAGGAGAAGACATGGGATCTTGATGCAACTATGGGGAATAAGTTTAAGGAGCTTGAGACCATGGCAATGGAATGTAACCAGGCTATGAGGAGGTTGCCATTCTTCCCCTTTTGTATACTTATTTTTGTCATCATTTAGTTATGGTTTTCCAATGTCTCAAATTTGATAGCTTCATATACAGTGGAATTGAGTTTAAGTTTATTTTCACTCCTTGTGGGTCGTTAGTTTGAAACTGTAATAGACAAGAATCTTATCAACTGAGAATAAGTTTGAATTATATTGAATTGTTTGGGAAGTTTCTGGATTTGATTGTTAGTTATGCTCACTTTTCTTTTTTCGTGAATCCGGCATTTTATGGAACTTATCCATCTCAAATGATACTGTTGAGCATGATCTCTTTGAATTTCTGCAAATAATTTGTCAGTGGATTCAAATTTTCTTATACTAATTGGAATTTTTCCTTAACAACCTTACTTGAACAGAATTTGTTCTATAGGTTGTACAACTATGTCCTTGAGTTCTAAAATTGAAAATTTTCCTTATAAATTAATCATAATTACTCTCCTGATACAACTTCATTTTATTATTATTAGATTGAAGCTTGATAATTGTCATCAGTATTTACTGAATGCAAAGGGGTCGACGCCAACTGATGTAATGGAAACTGACTATAAGACAACCGTTAAGCCTGCACTCAATTCCTTTGCAGAGGGCATAAAGAATAGTTCCATGATGAAGTTGGAGGATTTGATTTCTCTCAGGCAACAATGAAGTGAAAATACTGCTAAAATTGAGTTGAGACTCAATCACGTAGCTTCCCTTCAATCCCATATTGATGAAGTAAGCACATGACAGAAAGATATTGGCACTTGATTCGAGACGTGTCAGTTGTCTTACTTTCCGCATGTGCTTAATGCAAGATTTTTTTGTTGCCTACGAGCGGAGTTTCTTATTAGTTATTACAGTCTGCTGCCAGACTTGAAAATTGGCTGCAAATAACTTCATTTTATTTCATCATTTCTTTTATCATTTTGTTGACGAAGGTTTTGCTTGGCTTCTAGGAACTACCTCGTACAAAATGGTTTGCTAAACGTGTTAATGGTTTCTATCTTCTAGGTCGAAACACAACTAAACTCCTTAAAAGATGAAATAAGAGATCATACATTGAAATGCTCGACAGAATCTGAAAAGATGGTGGACGATGCTCAACAAGAAGCGCATAATTTGGATCTTGTGGAAAAAGAAACAGCAGTGATTTTAAAGGTATTAATACTGCTACCATAATTATTGTCTTAATTTATTTTTGGGGGTGGGGGTGGGGGTGGGGGTGGGTGTGAGGGAATTCGAATAGTCTCTTTTTTTTTATGAGATGACCTCAAAAATATGGTTATTAAGTCTATCGAACTTAAGGATTAAACACAAAGTTTTATCAAACTTAGGGACCAAGGGTGAATTTTACCTTCTTATCACTTACAATTGGGATTAAAATCTCAAAATTATGAAATATACTATACTATATGTTATATTAAGGATATCATTGTAATAAGTTTAGGTAATGAGAATAAAAATTGGTGGATTGATAACTTGTTTTTTCTTTCTAAAATTTGTTTGTAATTAAATCTAGATTTAAATGGCTTCAATGAAACTTCATTTCTATTCGGTTCTCAATGTTAAAAAGTGGCATGTTTGATTCACATGATTTGAATTTGATTTTTAATTGGTCTCTAAAGATTGATATTAGTTCATCAGTTTATGGTACCTACCATAAGTGAGTCGATTACTAGCTAATATGTTAGAATGGTGACTAACAACTAAAATATACTTACTAGATTTTGCTAATATGCATTTATATATGAGTTAGATTAGTTTTATCCAAAATCTAGATGGACTGGTAATCGATCTTTCGTTAACACAACCATATCAGTCTTCTGAAAGATCTCTAGAAGCATTGTTCCATCTCATTGAATCTTGTCTTACACCTAAACTAGGATGGAAAACAGTTAAGTATACTAACTGTCATAAGACTTTCCACCACCTCAGTTTCGTTTATTTAATTTATACTTGATGCTAGAATCTCCTCCATTCTTTTTGTCATTCCTTGTTGTTCTGTGAAGAATGCCAATTTGAAGCATCAGGAAGCTATAAAGAATTTGTTGGATCCAAAATTTCAGATATGAAAAGCTACCTCTCAAACACTGCTGTCGGTATCTCTGAGGTTTACAAGAATTCTTCGCCTGCCCTGCAGAATTGA

mRNA sequence

ATGAAGCCAACTCGCCGCCGCCGTCCAAATGGGTCTTTCAATCCTCCACCGCCGTCTACTTCCCAATTCCGCCGCGATTCAGATGTCAACTTTTCCAGTACCTTTCAAATTTCTGCTATCAACTCCATCAATTCGTCTCTCAAGGAGCTCTTCTCTCACTCCTTCAACGTAACATTCAAACTCTTCGCTCCTCCTTCTCTCAAAGATCTCACTGAAACCATGCATTTGCTTTTTCAATGTCTCGATTATCGCTTGACTAAGCTTGAGGACGATCTCCCCGTTGTCCTCAAATGCCTTTGGTATCCCTTCAAAATTAATAAAGTGGCACCAAATACTTGCCTAATTCACTGGCTTGTGCAGGTCGCTGTTTATAACTATAACCTTGCAAACGATTCGGATTCGTTTTTTACGTGCAACGAAGTGAATATATATGTGCTGAATAGCTATGTGCAATATATTCGAGGGGATGATGATTCGGTGGAGGCTTTGGATCAGGAGTTTATGGAGCGGTTGAAGAAAGGGAGGGATGAGGCTGTTGAAAGCTTGAAGGAATTGGAGAAGGAAGCTAAGGAGCTTGAAGCCAAAGTCGAGGCCTTGCGAGATCATACATTGAAATGCTCGACAGAATCTGAAAAGATGGTGGACGATGCTCAACAAGAAGCGCATAATTTGGATCTTGTGGAAAAAGAAACAGCAGTGATTTTAAAGAATCTCCTCCATTCTTTTTGTCATTCCTTGTTGTTCTGTGAAGAATGCCAATTTGAAGCATCAGGAAGCTATAAAGAATTTGTTGGATCCAAAATTTCAGATATGAAAAGCTACCTCTCAAACACTGCTGTCGGTATCTCTGAGGTTTACAAGAATTCTTCGCCTGCCCTGCAGAATTGA

Coding sequence (CDS)

ATGAAGCCAACTCGCCGCCGCCGTCCAAATGGGTCTTTCAATCCTCCACCGCCGTCTACTTCCCAATTCCGCCGCGATTCAGATGTCAACTTTTCCAGTACCTTTCAAATTTCTGCTATCAACTCCATCAATTCGTCTCTCAAGGAGCTCTTCTCTCACTCCTTCAACGTAACATTCAAACTCTTCGCTCCTCCTTCTCTCAAAGATCTCACTGAAACCATGCATTTGCTTTTTCAATGTCTCGATTATCGCTTGACTAAGCTTGAGGACGATCTCCCCGTTGTCCTCAAATGCCTTTGGTATCCCTTCAAAATTAATAAAGTGGCACCAAATACTTGCCTAATTCACTGGCTTGTGCAGGTCGCTGTTTATAACTATAACCTTGCAAACGATTCGGATTCGTTTTTTACGTGCAACGAAGTGAATATATATGTGCTGAATAGCTATGTGCAATATATTCGAGGGGATGATGATTCGGTGGAGGCTTTGGATCAGGAGTTTATGGAGCGGTTGAAGAAAGGGAGGGATGAGGCTGTTGAAAGCTTGAAGGAATTGGAGAAGGAAGCTAAGGAGCTTGAAGCCAAAGTCGAGGCCTTGCGAGATCATACATTGAAATGCTCGACAGAATCTGAAAAGATGGTGGACGATGCTCAACAAGAAGCGCATAATTTGGATCTTGTGGAAAAAGAAACAGCAGTGATTTTAAAGAATCTCCTCCATTCTTTTTGTCATTCCTTGTTGTTCTGTGAAGAATGCCAATTTGAAGCATCAGGAAGCTATAAAGAATTTGTTGGATCCAAAATTTCAGATATGAAAAGCTACCTCTCAAACACTGCTGTCGGTATCTCTGAGGTTTACAAGAATTCTTCGCCTGCCCTGCAGAATTGA

Protein sequence

MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSSTFQISAINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKCLWYPFKINKVAPNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLNSYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCSTESEKMVDDAQQEAHNLDLVEKETAVILKNLLHSFCHSLLFCEECQFEASGSYKEFVGSKISDMKSYLSNTAVGISEVYKNSSPALQN

Homology

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BLAST of PI0015309 vs. ExPASy Swiss-Prot
Match: Q9M1G5 (Kinetochore protein NDC80 homolog OS=Arabidopsis thaliana OX=3702 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.1e-19
Identity = 81/252 (32.14%), Postives = 128/252 (50.79%), Query Frame = 0


Query: 11  GSFNPPPPSTSQFR-----RDSDVNFSST--------------FQISAINSINSSLKELF 70
           G+  PPPPS  Q R     RDSD +F+S+               + S I  IN+ L    
Sbjct: 17  GAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASDDRSSMIRFINAFLS--- 76

Query: 71  SHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRL--TKLEDDLPVVLKCLWYPFKINK-- 130
           +H+F ++ +    PS+KD++ET+  L   LDY     K ++DL   LK    PFKI K  
Sbjct: 77  THNFPISIRGNPVPSVKDISETLKFLLSALDYPCDSIKWDEDLVFFLKSQKCPFKITKSS 136

Query: 131 -VAPNT--------CLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLNSYVQYIRGDDD 190
             APNT         ++HWL ++A ++ +L ++S S    N +N + + S+  +IRG+DD
Sbjct: 137 LKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTSVPEDNSMNFFAIQSFGHFIRGEDD 196

Query: 191 SVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCSTESEKMVDDAQ 231
            V  LD +F+ +L+  +    E++   EK + ELEAK+E+LR    K     EK+  D +
Sbjct: 197 KVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEAKLESLRKGPSK-KESLEKVKADLE 256

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BLAST of PI0015309 vs. ExPASy TrEMBL
Match: A0A0A0L740 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G213500 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.7e-89
Identity = 203/339 (59.88%), Postives = 231/339 (68.14%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK T RRRP  SFNPPPP T QFRRDSDV+F+S+                       QIS
Sbjct: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
            +N+INSSLKELFSHSFNVTFK F+PPSLKDLTET++LL QCLDY  TKLEDDLP++LK 
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LWYPFKINK------VAPN-----TCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK       AP+       L+HWLVQVA+YNYNLANDS+  FT ++VNIYVLN
Sbjct: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RD++ ESLKELE+E K LEAKVEALR     CS
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALR-----CS 240

Query: 241 TESEKMVDDAQQEAHNLDLVEKETAVILKNL--------------LHSFCHSLLFCEECQ 293
           TES+KMV+D QQEAHNLDLVE E    LKN               +H     LL C    
Sbjct: 241 TESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSC---- 300

[Close]

BLAST of PI0015309 vs. ExPASy TrEMBL
Match: A0A6J1JAC2 (kinetochore protein NDC80 homolog OS=Cucurbita maxima OX=3661 GN=LOC111482699 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 6.0e-73
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. ExPASy TrEMBL
Match: A0A6J1E5Y9 (kinetochore protein NDC80 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430885 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 6.0e-73
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. ExPASy TrEMBL
Match: A0A1S3AYB0 (kinetochore protein NDC80 homolog OS=Cucumis melo OX=3656 GN=LOC103484089 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 2.6e-68
Identity = 160/266 (60.15%), Postives = 187/266 (70.30%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK T RRRP  SFNPPPP T QFRRDSDV+F+S+                       QIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
            +N+INSSLKELFSHSFNVTFK F+PPSLKDLTET++LL QCLDY  TKLEDDLP++LK 
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LWYPFKINK------VAPN-----TCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK       AP+       L+HWLVQVA+YNYNLANDS+  F  ++VNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SY+ Y+RGDDDSVEALDQEFMERLKK RD++ ESLKELE+E K LEAKVEALR      S
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALR------S 240

[Close]

BLAST of PI0015309 vs. ExPASy TrEMBL
Match: A0A5D3DHD6 (Kinetochore protein NDC80-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00670 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 2.6e-68
Identity = 160/266 (60.15%), Postives = 187/266 (70.30%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK T RRRP  SFNPPPP T QFRRDSDV+F+S+                       QIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
            +N+INSSLKELFSHSFNVTFK F+PPSLKDLTET++LL QCLDY  TKLEDDLP++LK 
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LWYPFKINK------VAPN-----TCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK       AP+       L+HWLVQVA+YNYNLANDS+  F  ++VNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SY+ Y+RGDDDSVEALDQEFMERLKK RD++ ESLKELE+E K LEAKVEALR      S
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALR------S 240

[Close]

BLAST of PI0015309 vs. NCBI nr
Match: KAG6576966.1 (Kinetochore protein NDC80-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014987.1 Kinetochore protein NDC80-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-72
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. NCBI nr
Match: XP_022984383.1 (kinetochore protein NDC80 homolog [Cucurbita maxima])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-72
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. NCBI nr
Match: XP_023553517.1 (kinetochore protein NDC80 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-72
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. NCBI nr
Match: XP_022923126.1 (kinetochore protein NDC80 homolog [Cucurbita moschata])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-72
Identity = 165/266 (62.03%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST----------------------FQIS 60
           MK   RR P GSFNPPPP T QFRRDSD +F+S+                       QIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVLKC 120
           A+N+INSSLKELF+HSFN+TFK F+PPSLKDLTET+HLL QCLDY  TKLEDDLP+VLK 
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LWYPFKINKVA-----------PNTCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLN 180
           L YPFKINK             P   L+HWLVQVAVYNYNLANDSDSF + N+VN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCS 234
           SYVQYIRGDDDSVEALDQEFMERLKKGRD+AVESL  LE E KELEAK EALR      S
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALR------S 240

[Close]

BLAST of PI0015309 vs. NCBI nr
Match: XP_038884546.1 (kinetochore protein NDC80 homolog [Benincasa hispida])

HSP 1 Score: 278.5 bits (711), Expect = 6.7e-71
Identity = 217/569 (38.14%), Postives = 242/569 (42.53%), Query Frame = 0


Query: 1   MKPTRRRRPNGSFNPPPPSTSQFRRDSDVNFSST------------------------FQ 60
           MK T  R   GSFNPPPP T QFRRDSDV+F+S+                         Q
Sbjct: 3   MKRTGCRHRKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMARPAAASSSINMYKERAVQ 62

Query: 61  ISAINSINSSLKELFSHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRLTKLEDDLPVVL 120
           ISA+NSINSSLKELFSHSFNVTFK F+PPSLKDLT+T+HLL QCLDY  TKLEDDLP++L
Sbjct: 63  ISAVNSINSSLKELFSHSFNVTFKPFSPPSLKDLTDTIHLLLQCLDYHSTKLEDDLPIIL 122

Query: 121 KCLWYPFKINK------VAPN-----TCLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYV 180
           K L YPFKINK       AP+       L+HWLVQV+VYNYNLANDS+SF T N+VNIY 
Sbjct: 123 KSLGYPFKINKSILKNPPAPHQWYQILALLHWLVQVSVYNYNLANDSNSFITGNDVNIYA 182

Query: 181 LNSYVQYIRGDDDSVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEAL------ 240
           LN+YVQYIRGDDD VEALDQEFMERLKK RD+ V SLKELE+E KELEAK EAL      
Sbjct: 183 LNNYVQYIRGDDDLVEALDQEFMERLKKERDDVVVSLKELEEEVKELEAKAEALWSNPSQ 242

Query: 241 ------------------------------------------------------------ 296
                                                                       
Sbjct: 243 REILEKQQSLLEEDVKKFHTMIAGYSEKMQEIKKNLEEKEKELEVKVQENKKICEEHGEL 302

[Close]

BLAST of PI0015309 vs. TAIR 10
Match: AT3G54630.1 (CONTAINS InterPro DOMAIN/s: Kinetochore protein Ndc80 (InterPro:IPR005550); Has 24780 Blast hits to 15608 proteins in 1321 species: Archae - 545; Bacteria - 2969; Metazoa - 12597; Fungi - 2181; Plants - 1581; Viruses - 39; Other Eukaryotes - 4868 (source: NCBI BLink). )

HSP 1 Score: 97.8 bits (242), Expect = 1.5e-20
Identity = 81/252 (32.14%), Postives = 128/252 (50.79%), Query Frame = 0


Query: 11  GSFNPPPPSTSQFR-----RDSDVNFSST--------------FQISAINSINSSLKELF 70
           G+  PPPPS  Q R     RDSD +F+S+               + S I  IN+ L    
Sbjct: 17  GAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASDDRSSMIRFINAFLS--- 76

Query: 71  SHSFNVTFKLFAPPSLKDLTETMHLLFQCLDYRL--TKLEDDLPVVLKCLWYPFKINK-- 130
           +H+F ++ +    PS+KD++ET+  L   LDY     K ++DL   LK    PFKI K  
Sbjct: 77  THNFPISIRGNPVPSVKDISETLKFLLSALDYPCDSIKWDEDLVFFLKSQKCPFKITKSS 136

Query: 131 -VAPNT--------CLIHWLVQVAVYNYNLANDSDSFFTCNEVNIYVLNSYVQYIRGDDD 190
             APNT         ++HWL ++A ++ +L ++S S    N +N + + S+  +IRG+DD
Sbjct: 137 LKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTSVPEDNSMNFFAIQSFGHFIRGEDD 196

Query: 191 SVEALDQEFMERLKKGRDEAVESLKELEKEAKELEAKVEALRDHTLKCSTESEKMVDDAQ 231
            V  LD +F+ +L+  +    E++   EK + ELEAK+E+LR    K     EK+  D +
Sbjct: 197 KVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEAKLESLRKGPSK-KESLEKVKADLE 256

The following BLAST results are available for this feature:

BLAST of PI0015309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1G5	2.1e-19	32.14	Kinetochore protein NDC80 homolog OS=Arabidopsis thaliana OX=3702 GN=NDC80 PE=1 ...	[more]

BLAST of PI0015309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L740	2.7e-89	59.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G213500 PE=3 SV=1	[more]
A0A6J1JAC2	6.0e-73	62.03	kinetochore protein NDC80 homolog OS=Cucurbita maxima OX=3661 GN=LOC111482699 PE...	[more]
A0A6J1E5Y9	6.0e-73	62.03	kinetochore protein NDC80 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430885 ...	[more]
A0A1S3AYB0	2.6e-68	60.15	kinetochore protein NDC80 homolog OS=Cucumis melo OX=3656 GN=LOC103484089 PE=3 S...	[more]
A0A5D3DHD6	2.6e-68	60.15	Kinetochore protein NDC80-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of PI0015309 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576966.1	1.2e-72	62.03	Kinetochore protein NDC80-like protein, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022984383.1	1.2e-72	62.03	kinetochore protein NDC80 homolog [Cucurbita maxima]	[more]
XP_023553517.1	1.2e-72	62.03	kinetochore protein NDC80 homolog [Cucurbita pepo subsp. pepo]	[more]
XP_022923126.1	1.2e-72	62.03	kinetochore protein NDC80 homolog [Cucurbita moschata]	[more]
XP_038884546.1	6.7e-71	38.14	kinetochore protein NDC80 homolog [Benincasa hispida]	[more]

BLAST of PI0015309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54630.1	1.5e-20	32.14	CONTAINS InterPro DOMAIN/s: Kinetochore protein Ndc80 (InterPro:IPR005550); Has ...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 168..202
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR46681:SF1	KINETOCHORE PROTEIN NDC80 HOMOLOG	coord: 2..202 coord: 201..292
None	No IPR available	PANTHER	PTHR46681	KINETOCHORE PROTEIN NDC80 HOMOLOG	coord: 2..202 coord: 201..292
IPR038273	Ndc80 domain superfamily	GENE3D	1.10.418.30	Ncd80 complex, Ncd80 subunit	coord: 26..124 e-value: 3.8E-7 score: 32.2
IPR005550	Kinetochore protein Ndc80	PFAM	PF03801	Ndc80_HEC	coord: 12..130 e-value: 1.6E-9 score: 37.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0015309.1	PI0015309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051315	attachment of mitotic spindle microtubules to kinetochore
cellular_component	GO:0031262	Ndc80 complex