PI0015283 (gene) Melon (PI 482460) v1

Overview
NamePI0015283
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptiontRNA pseudouridine synthase B
Locationchr09: 18690619 .. 18694630 (-)
RNA-Seq ExpressionPI0015283
SyntenyPI0015283
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAAATCACCTGCTCTGTTCCACGCTTCCCATTTCTTCATGCGCTCTACACTCATGCCTTCTTCCAAATCACTCTCTCTCTTCCTTCCACTCTCAAATCTCAACCATCTTCACATGTTTGCCTTTCGCAATTCACTCTCTCCCTTATCCCTCCGTAGATTCTCCACCACTTCCACTCCATTCCCTCTCCTATACGAATTGATTATCAACCGACCGTCTTATCCTTCTCCGCCCCATCAGAATCCACGAACACCCACCAGAATCAGCTCCGACAACTCTCCCGAGCTCAACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTCGATCGGAAGCTTATATCCGAGAGTGGGACTGTTTCTGGTAAGGAAGGTGTGGTCATGGATAAGGCTATGAGGAAATACTATAATAAAAGAAGGAAGAGGATGTATGGGTCGGATTCTGATGAAGATAATAAGACACGGGATGAGGGATTTGTGGAGTTAAAGCCTGAGGTGGTTGAGTTTAGGACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTACGACGCTTTCGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGCATCAATTGGAGAAGAAATATTTTCCTGATGAAGGTCTCGACAAAGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTAAAGCCTGAGACGAACGTGGAGGTCGCCAATGGCATGGATGACAAAGGGATGGTTTTCTTTGATGAAGGGAAACCAGAAAAGGAGAATAAAGGGTCTGTAATAGATGTGACAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAGGGTCCTGCTAAAGATTCTGAAATTGGTCAGGCGGGGCCATATCTTTTGACGAGGCATATGGAGCTCCCGGCTAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGTTAGTCTGTGGTTTCTGTAGTCAAAGGAAATACCCTGTTTATAATCTTAGTGAAAGTTCTGTATCCATTTACATGTCTTCTAGTTTGCATTGTTCCAGTTAGAAGTAAATTCCTCACTCTTTTTCTTTTTGACGTTTAAAGAGAACAACGTGATTGTTTAACGTAGGATGGACTTCATTTATGGTATGTGGAAAGCTTCGGCGCCTAGTCAAAGTGCAAAAGGTGCATTTTTCAGCTGTTTCATCTGCATTAGACTAATTATTAGATATCCTGATCTTCTCTAACTACATTGTTTTCCACTTATGTGCATTGATAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCAACGAAGTTGGTTGAAAGGTGATGATTAAGTCAGCTATGTAATTGCTACTTTTTTTTTTTTCATTTCCTCCCTTTTTATAAGTGAGATATACTTTTGTTAATAGAAAATAATAATAATAAGAAGAAGAAATAAAGAGTAAGAGAAGACATGATCCCAAGCCAAAGTTAAAAAAGATTTCCAAATCCCCAGTACTGTCATTGGTGAAATGCAAAGAGTAGTTATAGCAAACCTGAGGGTACAACTTTTCTAAGAGGTGGCATGTTTATCTTTTAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTGTGTGATTTTATCTCTCTAGTCAATTATGGTTTCTTATTTTTAACTGATATGCATCTGTGTATTATATCTGATGTGTTCTTTTGGTAAGTATTATCTCTTTACTATATCTACTGTTAATGGATCTGCCAGAGGAATCATTATTCTAGTCTTAAGGGAGAGGATTTATGGTTCCATATTTTAACCAATCAAATATTTGCCTCTTGCAAGCCCGGTCACAATAGGGATGACAAAATTTTGGAAGTATATTAAATATAAAGGGAGGCTTCATTTTACATGCACCCTATCCATTCTCTACATCATTAGCATAATGTTTCTCAACATTCTGTTAGTTGTTGAGTTATTAGGTTAATTGCAAGGTGAGGTTTTGACTTGTCTCACAGGTTAATTAAAAAAGTTTCTATTGGGTTTCTGTTCCTGATGAAAAGTTATTTGTATAACATCCAAATAAAAATCATTTGTCCTAATTATTTTGCAGGTAATTCAGCGAGAGCCTTGGGAGCATATCAAAGATGATGACATCCAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTAAGCACTTCTTCTTTCTATAATTATAACTTTGAACATTCTTCAATATATATATATATATATATATAACTTTGAATAGTAGAAGCAATGTTGTTAAAAATGCATGTTTCACATGACAAAGCAAACACATTATTCGTGTCATATCTGTATGGAATTTTTACAACTTTGTTTAAAAACAAGTTTGTTTCTGTGTCAAAACATATGATGTTGCAGTTATCTGATTGTATAATGTTTTTGCCGAGCCAGCCAATTAGAATGACAGTATTTCATGCTTCCTCCATTCATAAATTATGAGTTACTTGTCCTCTAAAGATATGAAATTGATGTTTCAGCAGATCTTGGATGCACGAGTCCATTTTTATTACAAATTTCATTACCTTTTTTTTAGGTTGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCCCCTAGACAGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAAATGACAGGTAGGTTTACTTCCAACTCACACTTCTGTTTCATCATTTCTGTAACTTGTGGCTAGGCCATATGCAAATGCACCGAGCACTTGAAGATGTCCAATTGGTTGTTGACAGAGAGAAATTTATCTAGCTAAAATTGTGTCATTTCACTATATAAGATAGCTCTAGATCATTAGGTGGCATGGGTGTAGAAACTTCTGTTCTGATGTGAACCCAACTTTCATGTAGAAAGATGAAAGTAGTAAAAGAAAGAGACCATATAGAAATACAGGCCAAAATAAGTCTTGAAAGAGTTTCAGGAAAAGACTCCAATATCCAATGATTAAACATGAAGGAAAATCACAAAGGTTCCAACTTTTCAAAAAATGACTCTTTATATTTGATAAGGCCAACAACTTGACTCGGTTGTTAACTTGATGAGCTCTGGCTGGTTTGTCGGACAGCTCAAATATACTTGTTAGTTATTTCAAAATTTCAGTGGAAAATGTTGTTTTGTTGCACTACTAATATGTGTCAACTTCTTGATTTAGTGAATATGTTTTCTTTTAAAGATTTTAATGTGTACTAGCTGATGGGACTGTGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGCTCTCGGCAGGTATTCATCTTCCCCCATCCAATGGACCTTCATTTCTCTACATTCTTTTACCCTTTATAACCAAGAAATGCTTGAAAGAGTAAATCTGTGTCAAGCTTCTCGTTTGTTTTGATCCTCTCTAGTTTAATGTTGACAATGTAATATTCTTGTCTTTGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGTCAGTTCAAGAGTCTTTATGTATCTATTTTCTTAATGAGGCTCAAATGTGATCGGAAAGTCTCATTGAGATTTTAAAGAACACCATCAACTTGGTTATACTCTTCAATATCAAGTTCAGGAGAAAAGAAAAAAAAAAAACTTTAACAAGCTCAGTTGATCATATCAAAGAATCCCCTTTTCGGTTGTTTTATAAAGTACTTGAACTAAATATCGAAGACAATTTGATTTTGACACGAGCCTCCTCATTTCAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAGGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

mRNA sequence

ATGGCCAAATCACCTGCTCTGTTCCACGCTTCCCATTTCTTCATGCGCTCTACACTCATGCCTTCTTCCAAATCACTCTCTCTCTTCCTTCCACTCTCAAATCTCAACCATCTTCACATGTTTGCCTTTCGCAATTCACTCTCTCCCTTATCCCTCCGTAGATTCTCCACCACTTCCACTCCATTCCCTCTCCTATACGAATTGATTATCAACCGACCGTCTTATCCTTCTCCGCCCCATCAGAATCCACGAACACCCACCAGAATCAGCTCCGACAACTCTCCCGAGCTCAACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTCGATCGGAAGCTTATATCCGAGAGTGGGACTGTTTCTGGTAAGGAAGGTGTGGTCATGGATAAGGCTATGAGGAAATACTATAATAAAAGAAGGAAGAGGATGTATGGGTCGGATTCTGATGAAGATAATAAGACACGGGATGAGGGATTTGTGGAGTTAAAGCCTGAGGTGGTTGAGTTTAGGACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTACGACGCTTTCGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGCATCAATTGGAGAAGAAATATTTTCCTGATGAAGGTCTCGACAAAGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTAAAGCCTGAGACGAACGTGGAGGTCGCCAATGGCATGGATGACAAAGGGATGGTTTTCTTTGATGAAGGGAAACCAGAAAAGGAGAATAAAGGGTCTGTAATAGATGTGACAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAGGGTCCTGCTAAAGATTCTGAAATTGGTCAGGCGGGGCCATATCTTTTGACGAGGCATATGGAGCTCCCGGCTAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGATGGACTTCATTTATGGTATGTGGAAAGCTTCGGCGCCTAGTCAAAGTGCAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCAACGAAGTTGGTTGAAAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCATATCAAAGATGATGACATCCAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCCCCTAGACAGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAAATGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGCTCTCGGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAGGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

Coding sequence (CDS)

ATGGCCAAATCACCTGCTCTGTTCCACGCTTCCCATTTCTTCATGCGCTCTACACTCATGCCTTCTTCCAAATCACTCTCTCTCTTCCTTCCACTCTCAAATCTCAACCATCTTCACATGTTTGCCTTTCGCAATTCACTCTCTCCCTTATCCCTCCGTAGATTCTCCACCACTTCCACTCCATTCCCTCTCCTATACGAATTGATTATCAACCGACCGTCTTATCCTTCTCCGCCCCATCAGAATCCACGAACACCCACCAGAATCAGCTCCGACAACTCTCCCGAGCTCAACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTCGATCGGAAGCTTATATCCGAGAGTGGGACTGTTTCTGGTAAGGAAGGTGTGGTCATGGATAAGGCTATGAGGAAATACTATAATAAAAGAAGGAAGAGGATGTATGGGTCGGATTCTGATGAAGATAATAAGACACGGGATGAGGGATTTGTGGAGTTAAAGCCTGAGGTGGTTGAGTTTAGGACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTACGACGCTTTCGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGCATCAATTGGAGAAGAAATATTTTCCTGATGAAGGTCTCGACAAAGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTAAAGCCTGAGACGAACGTGGAGGTCGCCAATGGCATGGATGACAAAGGGATGGTTTTCTTTGATGAAGGGAAACCAGAAAAGGAGAATAAAGGGTCTGTAATAGATGTGACAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAGGGTCCTGCTAAAGATTCTGAAATTGGTCAGGCGGGGCCATATCTTTTGACGAGGCATATGGAGCTCCCGGCTAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGATGGACTTCATTTATGGTATGTGGAAAGCTTCGGCGCCTAGTCAAAGTGCAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCAACGAAGTTGGTTGAAAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCATATCAAAGATGATGACATCCAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCCCCTAGACAGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAAATGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGCTCTCGGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAGGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

Protein sequence

MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTSTPFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISESGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKREEELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVKKAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKDSEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLNDRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAITKGYF
Homology
BLAST of PI0015283 vs. ExPASy Swiss-Prot
Match: Q9RB36 (tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=truB PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.4e-48
Identity = 102/222 (45.95%), Postives = 138/222 (62.16%), Query Frame = 0

Query: 326 GTVVLLNKPKGWTSFMVCGKLRRLV-------KVQKVGHAGTLDPMATGLLIVCVGKATK 385
           G V+L++KP  W+SF    KL+ L+       K  K+GHAGTLDP+ATGLL++C GK TK
Sbjct: 9   GQVLLIDKPLKWSSFQAVNKLKYLLINKVGLPKKFKIGHAGTLDPLATGLLLICTGKFTK 68

Query: 386 LVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVP 445
            +   QG  K Y+G F +G  T ++D ++ + Q  P EHI +  I +    F GEI Q P
Sbjct: 69  KISELQGQAKEYTGTFYIGATTPSYDLETEIDQTFPTEHINEVLIHETVKQFLGEIDQKP 128

Query: 446 PMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLNDRQNLIFRVTCSKGTYIR 505
           P+FSAIK  G ++YE AR GESIE+  R+ +I +F+I R       + FRV CSKGTYIR
Sbjct: 129 PIFSAIKKDGVRLYEHARAGESIEIESRKTTIHEFEITRIA--LPEIDFRVVCSKGTYIR 188

Query: 506 SLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 541
           SL  D GKA+ S +HLT LRR  IG Y   +A +    E+++
Sbjct: 189 SLAYDFGKAMNSGSHLTVLRRTKIGDYDVKNAIDITLFEESL 228

BLAST of PI0015283 vs. ExPASy Swiss-Prot
Match: A0JUU8 (tRNA pseudouridine synthase B OS=Arthrobacter sp. (strain FB24) OX=290399 GN=truB PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.0e-47
Identity = 96/217 (44.24%), Postives = 138/217 (63.59%), Query Frame = 0

Query: 328 VVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMI 387
           +V+++KP+GWTS  V G++RRL   +KVGHAGTLDPMATG+L++ + KAT+L+    G  
Sbjct: 5   LVIVDKPQGWTSHDVVGRMRRLAGTRKVGHAGTLDPMATGVLVLGINKATRLLTYIVGTS 64

Query: 388 KSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVPPMFSAIKVG 447
           K+Y+   RLGE+T T DA+  V+       + +  I+ A A+  GEI QVP   SAIKV 
Sbjct: 65  KTYTATIRLGESTVTDDAEGEVVSSHSAAAVTEGAIRAAVAALTGEIQQVPSSVSAIKVN 124

Query: 448 GEKMYEKARRGESIELSPRQISIFKFDIERSLNDRQNLIF----RVTCSKGTYIRSLCAD 507
           GE+ Y + R GE ++L+ R ++I +FD+     +R   +      V CS GTYIR+L  D
Sbjct: 125 GERAYARVRSGEDVKLAARPVTIHRFDVHAVRPERAGAVLDVDVTVECSSGTYIRALARD 184

Query: 508 LGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 541
           LG+ALG+  HLTALRR  +G Y  D A   ++L + +
Sbjct: 185 LGEALGTGGHLTALRRTQVGPYTLDQARTLEQLAEEL 221

BLAST of PI0015283 vs. ExPASy Swiss-Prot
Match: A1R520 (tRNA pseudouridine synthase B OS=Paenarthrobacter aurescens (strain TC1) OX=290340 GN=truB PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.7e-46
Identity = 98/217 (45.16%), Postives = 134/217 (61.75%), Query Frame = 0

Query: 328 VVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMI 387
           +V+++KP+GWTS  V G++RRL   +KVGHAGTLDPMATG+L++ + KAT+L+    G  
Sbjct: 5   LVIVDKPQGWTSHDVVGRMRRLAGTRKVGHAGTLDPMATGVLVLGINKATRLLTYIVGTS 64

Query: 388 KSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVPPMFSAIKVG 447
           K+Y+   RLGE T T DA+  V +     HI DD +    A+  G I QVP   SAIKV 
Sbjct: 65  KTYTATIRLGETTITDDAEGEVTEARTAAHITDDAVAVGVAALTGPIQQVPSSVSAIKVN 124

Query: 448 GEKMYEKARRGESIELSPRQISIFKFDIERSLN-DRQNLI---FRVTCSKGTYIRSLCAD 507
           GE+ Y + R GE ++L+ R ++I +FD+      D   ++     V CS GTYIR+L  D
Sbjct: 125 GERSYARVRSGEEVKLAARPVTIHRFDVHSITRIDGGRVVDVDVTVECSSGTYIRALARD 184

Query: 508 LGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 541
           LG ALG   HLTALRR  +G Y  D A   +EL + +
Sbjct: 185 LGNALGIGGHLTALRRTQVGPYSLDQARTLEELAEEL 221

BLAST of PI0015283 vs. ExPASy Swiss-Prot
Match: Q47RU8 (tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=truB PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 6.5e-46
Identity = 97/220 (44.09%), Postives = 135/220 (61.36%), Query Frame = 0

Query: 328 VVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMI 387
           VV+++KP GWTS  V  ++RRL   ++VGHAGTLDPMATG+L+V VGKAT+L+       
Sbjct: 5   VVIVDKPAGWTSHDVVARVRRLAGTRRVGHAGTLDPMATGVLVVGVGKATRLLGYLALTE 64

Query: 388 KSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVPPMFSAIKVG 447
           K Y    RLG++T+T DA+  +++R P +HI +  +     +  G I QVPP  SA+KV 
Sbjct: 65  KVYEATIRLGQSTTTDDAEGELLERRPADHIDEAAVHAGTRALTGVIHQVPPQVSAVKVR 124

Query: 448 GEKMYEKARRGESIELSPRQISIFKFDIE--RSLNDRQNLIF----RVTCSKGTYIRSLC 507
           G++ Y +AR GE++EL  R++++ +F +   R ++           RVTCS GTYIRSL 
Sbjct: 125 GQRAYRRARAGETVELKAREVTVHEFTVTGFRRVDSPDGAFVDVDARVTCSSGTYIRSLA 184

Query: 508 ADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAIT 542
            DLG  LG   HLTALRR  +G Y  + A    EL    T
Sbjct: 185 RDLGADLGVGGHLTALRRTRVGPYTVEQAATLDELAAEFT 224

BLAST of PI0015283 vs. ExPASy Swiss-Prot
Match: Q5L0I5 (tRNA pseudouridine synthase B OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=truB PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 9.3e-45
Identity = 95/221 (42.99%), Postives = 138/221 (62.44%), Query Frame = 0

Query: 328 VVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMI 387
           V+LLNKPKG TS     K+RRL+ V+K GH GTLDP  +G+L +C+GKAT++ E   G  
Sbjct: 4   VLLLNKPKGMTSHDCVAKVRRLLGVKKAGHTGTLDPNVSGVLPICLGKATRIAEFLTGTT 63

Query: 388 KSYSGVFRLGEATSTWDADSPVIQREPWEH-IKDDDIQKAAASFCGEIWQVPPMFSAIKV 447
           K+Y G   LG AT+T DAD  +I   P +  I   +I+    S  GEI Q PPM+SA+KV
Sbjct: 64  KTYEGKVTLGAATTTEDADGDIIAARPVDRPIARAEIEAVFDSLTGEIEQTPPMYSAVKV 123

Query: 448 GGEKMYEKARRGESIELSPRQISIFKFDI----ERSLNDRQNLIFRVTCSKGTYIRSLCA 507
           GG+K+YE AR G  +E   R+++I++ ++    E+   +  +  FRVTCSKGTY+R+L  
Sbjct: 124 GGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFFGETVSFRFRVTCSKGTYVRTLAV 183

Query: 508 DLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAITKG 544
            +G+ LG  AH++ L R + G +  +D   F+++E     G
Sbjct: 184 MIGERLGYPAHMSDLIRTASGPFRLEDCVTFEDVERRAADG 224

BLAST of PI0015283 vs. ExPASy TrEMBL
Match: A0A1S3C8B0 (uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 7.6e-300
Identity = 515/545 (94.50%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSL LFLPLSNLNH H FAFRNSLSPLSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL YELIINRPSYPSPPHQNPRTP RISSDN PELNSSEDP+SELGF+SWVDRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSV DVTEKKVEEFFKCLKKGPAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S+IGQA PYLLTRHMELPA+WDSPCGTVVLLNKPKGWTSF VCGKLRRLVKVQKVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISIFKFDIERSL+
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQ+LADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQHLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TKGYF
Sbjct: 541 TKGYF 544

BLAST of PI0015283 vs. ExPASy TrEMBL
Match: A0A0A0KP03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.1e-297
Identity = 511/545 (93.76%), Postives = 528/545 (96.88%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSLSLFLPLSNLNHLH FAFRNSLSPLSLR FSTT T
Sbjct: 16  MAKSPALFHASHFFIRSTLMPSSKSLSLFLPLSNLNHLHRFAFRNSLSPLSLRTFSTT-T 75

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
            FPL YELIINRPSYPSPPHQNPRTP R+SSDNSPELNSSEDPTSELGF+SWVDRKLISE
Sbjct: 76  LFPLQYELIINRPSYPSPPHQNPRTPARVSSDNSPELNSSEDPTSELGFDSWVDRKLISE 135

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
            GTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+ EGFVELKPEVVEF TLHKRE
Sbjct: 136 GGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQAEGFVELKPEVVEFNTLHKRE 195

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GV+
Sbjct: 196 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVR 255

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KA RVKPE NVEVANGMDDK MVFFDEGKPEKENKGSV+DV+EKKVEEFFKCLKKGPAKD
Sbjct: 256 KAGRVKPEMNVEVANGMDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKD 315

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S IGQ  PYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 316 SNIGQGEPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 375

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 376 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 435

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDIQKAAASFCGEIWQVPPMFSAIKVGGE+MYEKARRGESIELSPRQISIFKFDIERSL+
Sbjct: 436 DDIQKAAASFCGEIWQVPPMFSAIKVGGERMYEKARRGESIELSPRQISIFKFDIERSLD 495

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 496 DRQNLIFRVTCSKGTYIRSLCADLGKTLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 555

Query: 541 TKGYF 546
           TKGYF
Sbjct: 556 TKGYF 559

BLAST of PI0015283 vs. ExPASy TrEMBL
Match: A0A5A7T7S0 (TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00150 PE=3 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 2.5e-287
Identity = 495/525 (94.29%), Postives = 512/525 (97.52%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSL LFLPLSNLNH H FAFRNSLSPLSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL YELIINRPSYPSPPHQNPRTP RISSDN PELNSSEDP+SELGF+SWVDRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSV DVTEKKVEEFFKCLKKGPAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S+IGQA PYLLTRHMELPA+WDSPCGTVVLLNKPKGWTSF VCGKLRRLVKVQKVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISIFKFDIERSL+
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQY 526
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQ+
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQF 524

BLAST of PI0015283 vs. ExPASy TrEMBL
Match: A0A6J1FDZ7 (uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC111444875 PE=3 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 4.1e-277
Identity = 481/545 (88.26%), Postives = 505/545 (92.66%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MA SPAL HASHFF+RSTLM SSKSL+ F PLSNLNHLH F   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL Y++II+RP YP  P QN RTP RIS DNSPEL SSEDP SELGFESWVD+KLISE
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           S + SGKEGVVMDKA RKYYNKRRKRMYGSDSDE+ KT+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMV+QLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAV VKPET VEV NG+D+KG+VFFD G+PEK+NKGSV DVTEKKVEEFFKCLKK PAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           SEIGQ GPYLLTRH ELPAKWDSPCGTVVL+NKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL +RYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISI+KFDIERSL+
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TK YF
Sbjct: 541 TKAYF 543

BLAST of PI0015283 vs. ExPASy TrEMBL
Match: A0A6J1JSH8 (uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.9e-277
Identity = 481/545 (88.26%), Postives = 504/545 (92.48%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MA SPAL HASHFF+RSTLM SSKSL+ F PLSNLNHLH F   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL Y++IIN P YP  P QN RTP RISSDNSPEL SSEDP SELGFESWVD+KLISE
Sbjct: 61  PFPLQYDMIINHPIYPPMPPQNRRTPPRISSDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           S + SGKEGVVMDKA RKYYNKRR+RMYGSDSDED KT+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRRRMYGSDSDEDKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMV+QLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAV VKPET VEV NG+D+KG+VFFD G+PEK+NKGSV DVTEKKVEEFFKCLKK PAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           SEIGQ  PYLLTRH ELPAKWDSPCGTVVL+NKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL +RYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISI+KFDIERSL+
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TK YF
Sbjct: 541 TKAYF 543

BLAST of PI0015283 vs. NCBI nr
Match: XP_008458830.1 (PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 515/545 (94.50%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSL LFLPLSNLNH H FAFRNSLSPLSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL YELIINRPSYPSPPHQNPRTP RISSDN PELNSSEDP+SELGF+SWVDRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSV DVTEKKVEEFFKCLKKGPAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S+IGQA PYLLTRHMELPA+WDSPCGTVVLLNKPKGWTSF VCGKLRRLVKVQKVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISIFKFDIERSL+
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQ+LADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQHLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TKGYF
Sbjct: 541 TKGYF 544

BLAST of PI0015283 vs. NCBI nr
Match: XP_011655389.2 (uncharacterized protein LOC101215528 isoform X1 [Cucumis sativus] >KGN51348.1 hypothetical protein Csa_008523 [Cucumis sativus])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.3e-297
Identity = 511/545 (93.76%), Postives = 528/545 (96.88%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSLSLFLPLSNLNHLH FAFRNSLSPLSLR FSTT T
Sbjct: 16  MAKSPALFHASHFFIRSTLMPSSKSLSLFLPLSNLNHLHRFAFRNSLSPLSLRTFSTT-T 75

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
            FPL YELIINRPSYPSPPHQNPRTP R+SSDNSPELNSSEDPTSELGF+SWVDRKLISE
Sbjct: 76  LFPLQYELIINRPSYPSPPHQNPRTPARVSSDNSPELNSSEDPTSELGFDSWVDRKLISE 135

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
            GTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+ EGFVELKPEVVEF TLHKRE
Sbjct: 136 GGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQAEGFVELKPEVVEFNTLHKRE 195

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GV+
Sbjct: 196 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVR 255

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KA RVKPE NVEVANGMDDK MVFFDEGKPEKENKGSV+DV+EKKVEEFFKCLKKGPAKD
Sbjct: 256 KAGRVKPEMNVEVANGMDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKD 315

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S IGQ  PYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 316 SNIGQGEPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 375

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 376 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 435

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDIQKAAASFCGEIWQVPPMFSAIKVGGE+MYEKARRGESIELSPRQISIFKFDIERSL+
Sbjct: 436 DDIQKAAASFCGEIWQVPPMFSAIKVGGERMYEKARRGESIELSPRQISIFKFDIERSLD 495

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 496 DRQNLIFRVTCSKGTYIRSLCADLGKTLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 555

Query: 541 TKGYF 546
           TKGYF
Sbjct: 556 TKGYF 559

BLAST of PI0015283 vs. NCBI nr
Match: KAA0037597.1 (TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 997.3 bits (2577), Expect = 5.3e-287
Identity = 495/525 (94.29%), Postives = 512/525 (97.52%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MAKSPALFHASHFF+RSTLMPSSKSL LFLPLSNLNH H FAFRNSLSPLSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL YELIINRPSYPSPPHQNPRTP RISSDN PELNSSEDP+SELGF+SWVDRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+T+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM++QLEKKYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSV DVTEKKVEEFFKCLKKGPAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           S+IGQA PYLLTRHMELPA+WDSPCGTVVLLNKPKGWTSF VCGKLRRLVKVQKVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISIFKFDIERSL+
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQY 526
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQ+
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQF 524

BLAST of PI0015283 vs. NCBI nr
Match: XP_038891059.1 (uncharacterized protein LOC120080471 [Benincasa hispida])

HSP 1 Score: 996.9 bits (2576), Expect = 6.9e-287
Identity = 496/545 (91.01%), Postives = 514/545 (94.31%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           M KS ALFHAS+FF+RST MPSS +L+LFL  SNLNHLH FAFRNSLSPLS RR STTST
Sbjct: 1   MVKSSALFHASNFFIRSTFMPSSITLTLFLLPSNLNHLHRFAFRNSLSPLSPRRLSTTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL YE+IINRPSYP PPHQN RTPTRI SDNSPE +SSEDPTSE+GFESWVDRKLISE
Sbjct: 61  PFPLQYEMIINRPSYPPPPHQNRRTPTRIRSDNSPEFDSSEDPTSEMGFESWVDRKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           S T+SGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKT+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SETISGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYDAFAYPWEKDKHYKMV+QLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK
Sbjct: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           K V V+PE  V+V +GMDDKG+VFFDEGKPE ENKGSV DVTEKKVEEFFKCLKK PAKD
Sbjct: 241 KGVGVEPEMKVKVTHGMDDKGLVFFDEGKPENENKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           SEIG   PYLLTRH ELPAKWDSPCGTVVLLNKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 301 SEIGPTEPYLLTRHTELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
            DI+KAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISIFKFDIERSL+
Sbjct: 421 GDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TKGYF
Sbjct: 541 TKGYF 545

BLAST of PI0015283 vs. NCBI nr
Match: XP_022938741.1 (uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 963.4 bits (2489), Expect = 8.4e-277
Identity = 481/545 (88.26%), Postives = 505/545 (92.66%), Query Frame = 0

Query: 1   MAKSPALFHASHFFMRSTLMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRFSTTST 60
           MA SPAL HASHFF+RSTLM SSKSL+ F PLSNLNHLH F   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLLYELIINRPSYPSPPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE 120
           PFPL Y++II+RP YP  P QN RTP RIS DNSPEL SSEDP SELGFESWVD+KLISE
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTRDEGFVELKPEVVEFRTLHKRE 180
           S + SGKEGVVMDKA RKYYNKRRKRMYGSDSDE+ KT+DEGFVELKPEVVEF TLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMV+QLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVIDVTEKKVEEFFKCLKKGPAKD 300
           KAV VKPET VEV NG+D+KG+VFFD G+PEK+NKGSV DVTEKKVEEFFKCLKK PAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGT 360
           SEIGQ GPYLLTRH ELPAKWDSPCGTVVL+NKPKGWTSF VCGKLRRLVKV+KVGHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL +RYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLN 480
           DDI+KA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPR+ISI+KFDIERSL+
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540
           DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

Query: 541 TKGYF 546
           TK YF
Sbjct: 541 TKAYF 543

BLAST of PI0015283 vs. TAIR 10
Match: AT5G14460.1 (Pseudouridine synthase family protein )

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-182
Identity = 356/543 (65.56%), Postives = 407/543 (74.95%), Query Frame = 0

Query: 19  LMPSSKSLSLFLPLSNLNHLHMFAFRNSLSPLSLRRF-STTSTPFPLLYELIINRPSYPS 78
           LM SS  +S+  PL       +  +RN   P     F STTST +PL Y++IINRP+  S
Sbjct: 10  LMSSSARMSMTSPLF---FSSVKPWRNIHKPYLASLFLSTTSTRYPLQYDMIINRPT-QS 69

Query: 79  PPHQNPRTPTRISSDNSPELNSSEDPTSELGFESWVDRKLISE-SGTVSGKEGVVMDKAM 138
              QN R P +     +P+       ++E  F+SWVD KL  E      G     MDKA 
Sbjct: 70  SLSQNRRRPPKAIESGAPD-------SAEPEFDSWVDNKLAMEREQGRPGSGDPEMDKAK 129

Query: 139 RKYYNKRRKRMYGSDSDEDNKTR--DEGFVELKPEVVEFRTLHKREEELFFYDAFAYPWE 198
           RKYY+KRRKR+YGSDS+++N +R  DEGFVELKPEVVEF  LH+REEEL+FYD FAYPWE
Sbjct: 130 RKYYSKRRKRLYGSDSEDENSSRKSDEGFVELKPEVVEFDRLHQREEELYFYDTFAYPWE 189

Query: 199 KDKHYKMVHQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVKKAVRVKPETNVEVA 258
           KDKHYKMV+QLEKKY+PD+ LDKAFL PGE   + ++  K R G KK V        EV 
Sbjct: 190 KDKHYKMVYQLEKKYYPDQCLDKAFLQPGEVLKKSDDSGKVR-GKKKVVAALGGKRSEVK 249

Query: 259 N-GM-----DDKGMVFFDEGKPEKENKGS---VIDVTEKKVEEFFKCLKKGPAKD---SE 318
             GM     DD  +VFFDE K ++E K S   V+ VTEKKVE+FFK L K P +    S 
Sbjct: 250 RIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQFFKGLTKSPNEKGMASG 309

Query: 319 IGQAGPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLD 378
            G   P+L+TR+ ELP +WD P GTV+L+NKPKGWTSF VCGKLRRLVKV+KVGHAGTLD
Sbjct: 310 GGDGEPFLVTRNGELPPRWDGPNGTVLLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLD 369

Query: 379 PMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDD 438
           PMATGLLIVCVGKATK+V+RYQGMIK YSGVFRLGEATST DADSPVIQRE WEHIKDDD
Sbjct: 370 PMATGLLIVCVGKATKVVDRYQGMIKGYSGVFRLGEATSTLDADSPVIQRESWEHIKDDD 429

Query: 439 IQKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRQISIFKFDIERSLNDR 498
           I+KA  SF GEIWQVPPMFSAIKVGGEKMYEKARRGE++ELSPR+ISIF+FDIERSL+DR
Sbjct: 430 IKKALTSFLGEIWQVPPMFSAIKVGGEKMYEKARRGETVELSPRRISIFQFDIERSLDDR 489

Query: 499 QNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQYLADDAWEFKELEDAITK 546
           QNLIFRV CSKGTYIRSLCADL KALGSCAHLTALRRDSIG+Y A+DAWEF ELE AITK
Sbjct: 490 QNLIFRVICSKGTYIRSLCADLAKALGSCAHLTALRRDSIGEYSANDAWEFNELEAAITK 540

BLAST of PI0015283 vs. TAIR 10
Match: AT3G57150.1 (homologue of NAP57 )

HSP 1 Score: 104.8 bits (260), Expect = 2.3e-22
Identity = 67/192 (34.90%), Postives = 101/192 (52.60%), Query Frame = 0

Query: 328 VVLLNKPKGWTSFMVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKATKLVERYQGMI 387
           V+ L+KP   +S  V   ++R+++V+K GH+GTLDP  TG LIVC+ +AT+LV+  QG  
Sbjct: 82  VINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAG 141

Query: 388 KSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQVPPMFSAIKVG 447
           K Y  V RL  A        P + +          + +A  S  G ++Q PP+ SA+K  
Sbjct: 142 KEYVCVARLHSAV-------PDVAK----------VARALESLTGAVFQRPPLISAVK-- 201

Query: 448 GEKMYEKARRGESIELSPRQISIFKFDIERSLNDRQNLIFRVTCSKGTYIRSLCADLGKA 507
                 +  R  +I  S     + ++D +R L     ++F V+C  GTYIR++C  LG  
Sbjct: 202 ------RQLRIRTIYES----KLLEYDADRHL-----VVFWVSCEAGTYIRTMCVHLGLL 239

Query: 508 LGSCAHLTALRR 520
           LG   H+  LRR
Sbjct: 262 LGVGGHMQELRR 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9RB361.4e-4845.95tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / ... [more]
A0JUU82.0e-4744.24tRNA pseudouridine synthase B OS=Arthrobacter sp. (strain FB24) OX=290399 GN=tru... [more]
A1R5201.7e-4645.16tRNA pseudouridine synthase B OS=Paenarthrobacter aurescens (strain TC1) OX=2903... [more]
Q47RU86.5e-4644.09tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=tru... [more]
Q5L0I59.3e-4542.99tRNA pseudouridine synthase B OS=Geobacillus kaustophilus (strain HTA426) OX=235... [more]
Match NameE-valueIdentityDescription
A0A1S3C8B07.6e-30094.50uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=... [more]
A0A0A0KP032.1e-29793.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1[more]
A0A5A7T7S02.5e-28794.29TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1FDZ74.1e-27788.26uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC1114448... [more]
A0A6J1JSH86.9e-27788.26uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372... [more]
Match NameE-valueIdentityDescription
XP_008458830.11.6e-29994.50PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo][more]
XP_011655389.24.3e-29793.76uncharacterized protein LOC101215528 isoform X1 [Cucumis sativus] >KGN51348.1 hy... [more]
KAA0037597.15.3e-28794.29TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N d... [more]
XP_038891059.16.9e-28791.01uncharacterized protein LOC120080471 [Benincasa hispida][more]
XP_022938741.18.4e-27788.26uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cu... [more]
Match NameE-valueIdentityDescription
AT5G14460.11.3e-18265.56Pseudouridine synthase family protein [more]
AT3G57150.12.3e-2234.90homologue of NAP57 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032819tRNA pseudouridylate synthase B, C-terminalPFAMPF16198TruB_C_2coord: 498..541
e-value: 4.2E-10
score: 39.6
IPR014780tRNA pseudouridine synthase II, TruBTIGRFAMTIGR00431TIGR00431coord: 327..536
e-value: 8.2E-66
score: 219.6
IPR014780tRNA pseudouridine synthase II, TruBPANTHERPTHR13767TRNA-PSEUDOURIDINE SYNTHASEcoord: 300..543
IPR014780tRNA pseudouridine synthase II, TruBHAMAPMF_01080TruB_bactcoord: 325..545
score: 15.61309
IPR014780tRNA pseudouridine synthase II, TruBCDDcd02573PseudoU_synth_EcTruBcoord: 328..539
e-value: 4.95272E-101
score: 302.056
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 319..544
e-value: 1.4E-87
score: 295.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..106
IPR002501Pseudouridine synthase II, N-terminalPFAMPF01509TruB_Ncoord: 347..497
e-value: 1.3E-52
score: 178.2
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 327..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015283.1PI0015283.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990481 mRNA pseudouridine synthesis
biological_process GO:0006400 tRNA modification
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
biological_process GO:0006396 RNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding