PI0015248 (gene) Melon (PI 482460) v1

Overview


Name	PI0015248
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	E3 ubiquitin-protein ligase RHA1B
Location	chr09: 1845858 .. 1846592 (+)
RNA-Seq Expression	PI0015248
Synteny	PI0015248

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTATAAATAAGAATTGGCCGTCTTTCTCCGGCCATATAATTCCATTAATCATCTCTTCCAAATTTCTCTCTTCTTCTTCTTCCTCTCTGTTTTACATTTCCGCCATGGGTTTCCCTGCAACTTACACTGAGCTTCTTCTCCCTAAGCTCTTCATCCATTTACTCTCCTTCCTCGGCTTCCTCCGTAAGCTTATCTCCTTCCTCTTCCGCCTCTTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCCCCCCGATTCCTTCCCCACTTCTCCCTTCACCCACCTCGATCCTCTCTCCGCCGCCCTTCTCCGTGAGATTCTCCCCGTCGTCAAGTTTTCCGACCTTCTCGACCCCCCTGACTGCTGCGCTGTTTGTCTCTACGAGTTCGAGTCCGACGATGAGATCCGCCGTCTCGCTAACTGTCGTCACATCTTCCACCGCGGATGCCTCGATCGATGGATCGGTTACGGTCACAGAACCTGCCCCCTGTGCCGGACTGTGTTTATTCCTCCTGATTTGAGAAGTGGTGGTTGTAATTTTGATGATAGGCTTTGGGAGGATTCGGAAATCTTTGAACTTCTCCACACCGATTCTTCTTCTTCAAATCTTCTCCCAACCGATGGTTTGTAGCTTTTGAAGATTTGATTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGATTTTGCTGTTCTGCTTCTTCTCATCAATCCATATCG

mRNA sequence

CTATAAATAAGAATTGGCCGTCTTTCTCCGGCCATATAATTCCATTAATCATCTCTTCCAAATTTCTCTCTTCTTCTTCTTCCTCTCTGTTTTACATTTCCGCCATGGGTTTCCCTGCAACTTACACTGAGCTTCTTCTCCCTAAGCTCTTCATCCATTTACTCTCCTTCCTCGGCTTCCTCCGTAAGCTTATCTCCTTCCTCTTCCGCCTCTTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCCCCCCGATTCCTTCCCCACTTCTCCCTTCACCCACCTCGATCCTCTCTCCGCCGCCCTTCTCCGTGAGATTCTCCCCGTCGTCAAGTTTTCCGACCTTCTCGACCCCCCTGACTGCTGCGCTGTTTGTCTCTACGAGTTCGAGTCCGACGATGAGATCCGCCGTCTCGCTAACTGTCGTCACATCTTCCACCGCGGATGCCTCGATCGATGGATCGGTTACGGTCACAGAACCTGCCCCCTGTGCCGGACTGTGTTTATTCCTCCTGATTTGAGAAGTGGTGGTTGTAATTTTGATGATAGGCTTTGGGAGGATTCGGAAATCTTTGAACTTCTCCACACCGATTCTTCTTCTTCAAATCTTCTCCCAACCGATGGTTTGTAGCTTTTGAAGATTTGATTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGATTTTGCTGTTCTGCTTCTTCTCATCAATCCATATCG

Coding sequence (CDS)

ATGGGTTTCCCTGCAACTTACACTGAGCTTCTTCTCCCTAAGCTCTTCATCCATTTACTCTCCTTCCTCGGCTTCCTCCGTAAGCTTATCTCCTTCCTCTTCCGCCTCTTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCCCCCCGATTCCTTCCCCACTTCTCCCTTCACCCACCTCGATCCTCTCTCCGCCGCCCTTCTCCGTGAGATTCTCCCCGTCGTCAAGTTTTCCGACCTTCTCGACCCCCCTGACTGCTGCGCTGTTTGTCTCTACGAGTTCGAGTCCGACGATGAGATCCGCCGTCTCGCTAACTGTCGTCACATCTTCCACCGCGGATGCCTCGATCGATGGATCGGTTACGGTCACAGAACCTGCCCCCTGTGCCGGACTGTGTTTATTCCTCCTGATTTGAGAAGTGGTGGTTGTAATTTTGATGATAGGCTTTGGGAGGATTCGGAAATCTTTGAACTTCTCCACACCGATTCTTCTTCTTCAAATCTTCTCCCAACCGATGGTTTGTAG

Protein sequence

MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSPFTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLDRWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSSNLLPTDGL

Homology

[Close]

BLAST of PI0015248 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.2e-43
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFP  YTE+ LPKLF+  LS LGF+R ++  +FR  GL DFLE D  WP+   S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPD-YTSYPTRI 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLL----DPPDCCAVCLYEFESDDEIRRLANCRHIFHR 120
                P SA L+REILPV+KF +L     D P+ CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFE 162
            CLDRW+ +  +TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of PI0015248 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-24
Identity = 61/143 (42.66%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MG P  + EL +P   +  L  +GF R ++  L    GL  FL+ +    + P     S 
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLL-DPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCL 120
                  SA L  E++PVV+FSDLL DP DCC VCL +F SDD+IR+L  C H+FH  CL
Sbjct: 61  ------TSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWIGYGHR-TCPLCRTVFIPPD 142
           DRWI   ++ TCP+CR  F+P +
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of PI0015248 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.1e-24
Identity = 60/144 (41.67%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 1   MGFPATY-TELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTS 60
           MG P  + TEL +P   + +L  +GF R ++  L    GL  FL+ +       ++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHN-------ETSAPD 60

Query: 61  PFTHLDPLSAALLREILPVVKFSDL-LDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGC 120
              H    SA+L  E++PVV+FSDL  DP DCC VCL +FESDD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGHRTCPLCRTVFIPPD 142
           LDRWI  Y    CP+CR  F+P +
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKE 137

[Close]

BLAST of PI0015248 vs. ExPASy Swiss-Prot
Match: Q9SZL4 (RING-H2 finger protein ATL18 OS=Arabidopsis thaliana OX=3702 GN=ATL18 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 90  CCAVCLYEFESDDEIRRLANCRHIFHRGCLDRWIGYGHRTCPLCRTVFIPP 141
           CC +CL EFE++D +  L  C H+FH  C++ W+  GH TCPLCR+  + P
Sbjct: 61  CCPICLVEFEAEDAVTHLPRCAHLFHINCIEPWLLRGHLTCPLCRSFVLAP 111

[Close]

BLAST of PI0015248 vs. ExPASy Swiss-Prot
Match: Q570X5 (RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.9e-11
Identity = 29/69 (42.03%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 72  LREILPVVKFSDLLDPPDC-CAVCLYEFESDDEIRRLANCRHIFHRGCLDRWIGYGHRTC 131
           LRE+LP+V F +     D  C+VCL +++ +D+++++  C+H FH  C+D W+   H TC
Sbjct: 80  LREMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFHMDCIDLWL-TSHTTC 139

Query: 132 PLCRTVFIP 140
           PLCR   IP
Sbjct: 140 PLCRLALIP 147

[Close]

BLAST of PI0015248 vs. ExPASy TrEMBL
Match: A0A0A0KQV2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.2e-97
Identity = 173/179 (96.65%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISF+FRLFGLQDFLEPDIPWPNPPDSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFIFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTDSSSSNLLPTDGL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LH+DSSSSNLL TDGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHSDSSSSNLLSTDGL 179

[Close]

BLAST of PI0015248 vs. ExPASy TrEMBL
Match: A0A5A7TMZ8 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002380 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-96
Identity = 175/185 (94.59%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTD------SSSSNLL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LHTD      SSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFERLHTDSSSSSSSSSSNLL 180

[Close]

BLAST of PI0015248 vs. ExPASy TrEMBL
Match: A0A1S3AU97 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.0e-95
Identity = 174/185 (94.05%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNP DSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPSDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTD------SSSSNLL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LHTD      SSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHTDSSSSSSSSSSNLL 180

[Close]

BLAST of PI0015248 vs. ExPASy TrEMBL
Match: A0A6J1H5J7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111459775 PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 5.9e-84
Identity = 150/178 (84.27%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS LF L GLQDFLEPDIPW N  DSFPT P
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVV+FS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSSNLLPTDGL 179
           RWIGYG RTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FE LHTDSSSS+ L + GL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of PI0015248 vs. ExPASy TrEMBL
Match: A0A6J1K664 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111491048 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-83
Identity = 148/171 (86.55%), Postives = 158/171 (92.40%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS LFRL GLQDFLEPDIPW N  DSFPT P
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVV+FS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSSN 172
           RWIGYG RTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FE LHTDSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS 171

[Close]

BLAST of PI0015248 vs. NCBI nr
Match: XP_004143977.2 (E3 ubiquitin-protein ligase RHA1B [Cucumis sativus] >KGN50076.1 hypothetical protein Csa_000207 [Cucumis sativus])

HSP 1 Score: 365.5 bits (937), Expect = 2.5e-97
Identity = 173/179 (96.65%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISF+FRLFGLQDFLEPDIPWPNPPDSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFIFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTDSSSSNLLPTDGL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LH+DSSSSNLL TDGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHSDSSSSNLLSTDGL 179

[Close]

BLAST of PI0015248 vs. NCBI nr
Match: KAA0042739.1 (E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa])

HSP 1 Score: 361.7 bits (927), Expect = 3.7e-96
Identity = 175/185 (94.59%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTD------SSSSNLL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LHTD      SSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFERLHTDSSSSSSSSSSNLL 180

[Close]

BLAST of PI0015248 vs. NCBI nr
Match: XP_008437313.1 (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo])

HSP 1 Score: 358.2 bits (918), Expect = 4.0e-95
Identity = 174/185 (94.05%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNP DSFPTSP
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPSDSFPTSP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFELLHTD------SSSSNLL 179
           RWIGYG RTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE LHTD      SSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHTDSSSSSSSSSSNLL 180

[Close]

BLAST of PI0015248 vs. NCBI nr
Match: XP_038906771.1 (E3 ubiquitin-protein ligase RHA1B [Benincasa hispida])

HSP 1 Score: 335.5 bits (859), Expect = 2.8e-88
Identity = 156/171 (91.23%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPA+YTELLLPKLFIH+LSFLGFLRKLIS LFRL GLQDFLEPDIPWPN PDSFPT P
Sbjct: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
           FT LDPLSAA+LREILPVVKFS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSSN 172
           RWIGYG RTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFE LHTDSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALHTDSSSSS 171

[Close]

BLAST of PI0015248 vs. NCBI nr
Match: XP_023549736.1 (E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.9 bits (821), Expect = 7.2e-84
Identity = 153/178 (85.96%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS LFRL GLQDFLEPDIPWPN  DSF T P
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVVKFS+LLDPP+ CAVCLYEFESDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSSNLLPTDGL 179
           RWIGYG RTCPLCRTVFIP DLRSG CNFDDRLWEDSEIFE LH+DSSSS+ L + GL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSSTLLSAGL 178

[Close]

BLAST of PI0015248 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 1.0e-48
Identity = 102/176 (57.95%), Postives = 121/176 (68.75%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPD---IPWPNPPDSFP 60
           MGFP  Y+ELLLPK+F +LLSFLG +RKLIS +F++ GL DFLEP+     WP+PP    
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPP--- 60

Query: 61  TSPFTHLDPLSAALLR-EILPVVKFSDLLDP-PDCCAVCLYEFESDDEIRRLANCRHIFH 120
               T   P SAA+L  E+LPVV+FSD+  P  +CCAVCLY+FE+DDEIRRL NCRHIFH
Sbjct: 61  ----TLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFH 120

Query: 121 RGCLDRW-IGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFELLHTDSSSS 171
           RGCLDRW +GY   TCPLCRT FIP  L+     F+ RLW  S        D SSS
Sbjct: 121 RGCLDRWMMGYNQMTCPLCRTQFIPDHLQ---LEFNQRLWSQSSAVSSQLLDESSS 166

[Close]

BLAST of PI0015248 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-44
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MGFP  YTE+ LPKLF+  LS LGF+R ++  +FR  GL DFLE D  WP+   S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPD-YTSYPTRI 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLL----DPPDCCAVCLYEFESDDEIRRLANCRHIFHR 120
                P SA L+REILPV+KF +L     D P+ CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFE 162
            CLDRW+ +  +TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of PI0015248 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 2.9e-43
Identity = 92/175 (52.57%), Postives = 115/175 (65.71%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPD-----IPWPNPPDS 60
           MG+P  YTELLLP++F+HLLS LG +R LI   FR+ GL DFLE D       W  PP  
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPP-Y 60

Query: 61  FPTSPFTHLD-----PLSAALLREILPVVKFSDLLDP-----PDCCAVCLYEFESDDEIR 120
             T+   H +     P++A L  EILPV++FS+L  P      DCCAVCL+EFE+DDEIR
Sbjct: 61  MSTAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIR 120

Query: 121 RLANCRHIFHRGCLDRW-IGYGHRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEI 160
           RL NC+HIFHR CLDRW +GY   TCPLCRT FI  +L+     F+ R+W +SE+
Sbjct: 121 RLTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVA---FNQRVWSESEL 171

[Close]

BLAST of PI0015248 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 113.6 bits (283), Expect = 1.6e-25
Identity = 61/143 (42.66%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60
           MG P  + EL +P   +  L  +GF R ++  L    GL  FL+ +    + P     S 
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  FTHLDPLSAALLREILPVVKFSDLL-DPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCL 120
                  SA L  E++PVV+FSDLL DP DCC VCL +F SDD+IR+L  C H+FH  CL
Sbjct: 61  ------TSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWIGYGHR-TCPLCRTVFIPPD 142
           DRWI   ++ TCP+CR  F+P +
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of PI0015248 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 112.5 bits (280), Expect = 3.6e-25
Identity = 60/144 (41.67%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 1   MGFPATY-TELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTS 60
           MG P  + TEL +P   + +L  +GF R ++  L    GL  FL+ +       ++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHN-------ETSAPD 60

Query: 61  PFTHLDPLSAALLREILPVVKFSDL-LDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGC 120
              H    SA+L  E++PVV+FSDL  DP DCC VCL +FESDD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGHRTCPLCRTVFIPPD 142
           LDRWI  Y    CP+CR  F+P +
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKE 137

The following BLAST results are available for this feature:

BLAST of PI0015248 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	2.2e-43	52.73	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	2.3e-24	42.66	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SUS4	5.1e-24	41.67	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SZL4	2.2e-11	47.06	RING-H2 finger protein ATL18 OS=Arabidopsis thaliana OX=3702 GN=ATL18 PE=2 SV=1	[more]
Q570X5	2.9e-11	42.03	RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1	[more]

BLAST of PI0015248 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQV2	1.2e-97	96.65	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 P...	[more]
A0A5A7TMZ8	1.8e-96	94.59	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3AU97	2.0e-95	94.05	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 S...	[more]
A0A6J1H5J7	5.9e-84	84.27	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1K664	1.3e-83	86.55	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114910...	[more]

BLAST of PI0015248 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143977.2	2.5e-97	96.65	E3 ubiquitin-protein ligase RHA1B [Cucumis sativus] >KGN50076.1 hypothetical pro...	[more]
KAA0042739.1	3.7e-96	94.59	E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa]	[more]
XP_008437313.1	4.0e-95	94.05	PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo]	[more]
XP_038906771.1	2.8e-88	91.23	E3 ubiquitin-protein ligase RHA1B [Benincasa hispida]	[more]
XP_023549736.1	7.2e-84	85.96	E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0015248 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63840.1	1.0e-48	57.95	RING/U-box superfamily protein	[more]
AT3G61460.1	1.6e-44	52.73	brassinosteroid-responsive RING-H2	[more]
AT5G41400.1	2.9e-43	52.57	RING/U-box superfamily protein	[more]
AT4G11360.1	1.6e-25	42.66	RING-H2 finger A1B	[more]
AT4G11370.1	3.6e-25	41.67	RING-H2 finger A1A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 91..133 e-value: 5.9E-6 score: 35.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 90..134 e-value: 4.0E-12 score: 46.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 91..134 score: 12.474524
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 66..139 e-value: 8.1E-17 score: 62.7
None	No IPR available	PANTHER	PTHR45969:SF46	BNAA09G11240D PROTEIN	coord: 1..157
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..157
None	No IPR available	CDD	cd16448	RING-H2	coord: 91..134 e-value: 5.24617E-14 score: 61.3139
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 76..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0015248.1	PI0015248.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding