PI0015029 (gene) Melon (PI 482460) v1

Overview
NamePI0015029
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionIsoaspartyl peptidase/L-asparaginase
Locationchr07: 22724968 .. 22726419 (+)
RNA-Seq ExpressionPI0015029
SyntenyPI0015029
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATAAGGCAGAGAGAGAGATTAAAAAAAAAAAAGAAACTGAAAGATGGGAGGTTGGGCCATTGCAGTTCATGGCGGGGCTGGGGTGGATCCTAATCTCCCATTGCATCGTCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTCGAACTCGTCGTAAGCTTTCCATTTTCTTCGCACTCACATTTATCTTTTTCTTATATGTAATTAACCAACAATTTGCCTCTCCATTTTGAAGGTTAGAGAATTGGAAAACGATCCCCTGTTCAACTCTGGCCGTGGATCTGCCCTCACCGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGATGGCCCTAAACGGAGATGCGGAGCGGTTTCTGGACTCACCACCGTAAAAAACCCCATCTCTCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCCTATCTTGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGTTTCATTCTGATTTCTTTTTATCCAATTTCGTAACCTCCAATTTTTCTGACACTTCCGATCGCCTCAGGGTGTAGAGCTTGTATGCAATGACTATTTCATCACCGAAGGAAACGTTGAACTGCTTCAACTAGCCAAAGAAGCCAACGGTATATGGGTAAGCCTAAGCCGGCCGCCATATAATCATTCATTCATTACAAATAACCGTCCGATCGAGAAACGGACTTTTAAAATTCGTTTGGTTTTTATTTGAAACAGTTGGATCGCCGGGTTCCAAGCGAGACATGTAGCGCGAGCGTTGAGACGCCATTGCAAATGAACGGTGTCCCAATCGGCGTGTACGCGCCGGAGACAGTGGGATGCGTGGTGGTGGATAGCGAAGGAAGGTGTGCCGCCGCAACGTCCACGGGTGGATTGATAAACAAAAAGATCGGACGTATCGGAGACTCGCCGTTGATCGGATTGGGGACGTACGCGTGTGATGTATGTGGGGTGTCGTGCACGGGAGAAGGTGAGGCGATCATAAGAGGGACATTGGCTCGAGAAGTGGCGGCGGTGATGGAATATAAAGGGTTAGGACTACAAGAGGCGGTAAATTATGTGATGGAAGAGAGATTGGATGAAGGGCAAGCAGGATTGATTGCAGTGTCTAGCAAAGGAGAAGTGGCATGCGGGTTCAACACGACCGGGATGTTTAGGGGATTCGCCACGGAGGATGGGTTTATGGAAGTTGGGATTTGGTAATCTCAATAGTTAGATTAACTAAGAATGGGAATGGGAGTAATTTGGTTTGGGATTTTAATTAATGTTTTTAGTTTGGCGTGTGTTCTGTTCTTGGTGAATCTTTTGTTCTACCTTTTTCTCTGAAGTTCAGATGCTATTTGTGTTAGCAGCAAATAATAAAGCCCTTAGTCCTAACCTTTTATTTAA

mRNA sequence

GAATAAGGCAGAGAGAGAGATTAAAAAAAAAAAAGAAACTGAAAGATGGGAGGTTGGGCCATTGCAGTTCATGGCGGGGCTGGGGTGGATCCTAATCTCCCATTGCATCGTCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTCGAACTCGTCGTTAGAGAATTGGAAAACGATCCCCTGTTCAACTCTGGCCGTGGATCTGCCCTCACCGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGATGGCCCTAAACGGAGATGCGGAGCGGTTTCTGGACTCACCACCGTAAAAAACCCCATCTCTCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCCTATCTTGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTAGAGCTTGTATGCAATGACTATTTCATCACCGAAGGAAACGTTGAACTGCTTCAACTAGCCAAAGAAGCCAACGGTATATGGTTGGATCGCCGGGTTCCAAGCGAGACATGTAGCGCGAGCGTTGAGACGCCATTGCAAATGAACGGTGTCCCAATCGGCGTGTACGCGCCGGAGACAGTGGGATGCGTGGTGGTGGATAGCGAAGGAAGGTGTGCCGCCGCAACGTCCACGGGTGGATTGATAAACAAAAAGATCGGACGTATCGGAGACTCGCCGTTGATCGGATTGGGGACGTACGCGTGTGATGTATGTGGGGTGTCGTGCACGGGAGAAGGTGAGGCGATCATAAGAGGGACATTGGCTCGAGAAGTGGCGGCGGTGATGGAATATAAAGGGTTAGGACTACAAGAGGCGGTAAATTATGTGATGGAAGAGAGATTGGATGAAGGGCAAGCAGGATTGATTGCAGTGTCTAGCAAAGGAGAAGTGGCATGCGGGTTCAACACGACCGGGATGTTTAGGGGATTCGCCACGGAGGATGGGTTTATGGAAGTTGGGATTTGGTAATCTCAATAGTTAGATTAACTAAGAATGGGAATGGGAGTAATTTGGTTTGGGATTTTAATTAATGTTTTTAGTTTGGCGTGTGTTCTGTTCTTGGTGAATCTTTTGTTCTACCTTTTTCTCTGAAGTTCAGATGCTATTTGTGTTAGCAGCAAATAATAAAGCCCTTAGTCCTAACCTTTTATTTAA

Coding sequence (CDS)

ATGGGAGGTTGGGCCATTGCAGTTCATGGCGGGGCTGGGGTGGATCCTAATCTCCCATTGCATCGTCAAGACGATGCCAAAAAACTTCTCACTCGCTGCCTTGATCTTGGCATCCACGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTCGAACTCGTCGTTAGAGAATTGGAAAACGATCCCCTGTTCAACTCTGGCCGTGGATCTGCCCTCACCGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGATGGCCCTAAACGGAGATGCGGAGCGGTTTCTGGACTCACCACCGTAAAAAACCCCATCTCTCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCCTATCTTGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTAGAGCTTGTATGCAATGACTATTTCATCACCGAAGGAAACGTTGAACTGCTTCAACTAGCCAAAGAAGCCAACGGTATATGGTTGGATCGCCGGGTTCCAAGCGAGACATGTAGCGCGAGCGTTGAGACGCCATTGCAAATGAACGGTGTCCCAATCGGCGTGTACGCGCCGGAGACAGTGGGATGCGTGGTGGTGGATAGCGAAGGAAGGTGTGCCGCCGCAACGTCCACGGGTGGATTGATAAACAAAAAGATCGGACGTATCGGAGACTCGCCGTTGATCGGATTGGGGACGTACGCGTGTGATGTATGTGGGGTGTCGTGCACGGGAGAAGGTGAGGCGATCATAAGAGGGACATTGGCTCGAGAAGTGGCGGCGGTGATGGAATATAAAGGGTTAGGACTACAAGAGGCGGTAAATTATGTGATGGAAGAGAGATTGGATGAAGGGCAAGCAGGATTGATTGCAGTGTCTAGCAAAGGAGAAGTGGCATGCGGGTTCAACACGACCGGGATGTTTAGGGGATTCGCCACGGAGGATGGGTTTATGGAAGTTGGGATTTGGTAA

Protein sequence

MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELENDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACGFNTTGMFRGFATEDGFMEVGIW
Homology
BLAST of PI0015029 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 517.7 bits (1332), Expect = 9.5e-146
Identity = 254/324 (78.40%), Postives = 287/324 (88.58%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAG+DPNLP  RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELE 
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETC--SASVETP 180
           AFSGAE FAR+QGVE+V N+YF+T+ NV +L+LAKEAN I  D R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVC 240
           +QMNG+PI +YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YV++ RLDEG AGLIAVS+KGEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGFATEDGFMEVGIW 323
           CGFN+ GMFRG ATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

BLAST of PI0015029 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-100
Identity = 196/328 (59.76%), Postives = 238/328 (72.56%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  NDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           N   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRR-----VPSETCSAS 180
           LAF GA+ FA+QQGVE V + +FIT  NVE L+LA EAN + +D        P++   A 
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQD-DAE 180

Query: 181 VETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYA 240
            E PL      IG     TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG GTYA
Sbjct: 181 KELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYA 240

Query: 241 CDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSK 300
            ++C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ 
Sbjct: 241 NELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAA 300

Query: 301 GEVACGFNTTGMFRGFATEDGFMEVGIW 323
           GE+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 GEIAMPFNTTGMFRASATEDGYSEIAIW 322

BLAST of PI0015029 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-98
Identity = 193/327 (59.02%), Postives = 235/327 (71.87%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  NDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           N   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLD---RRVPSET-CSASV 180
           LAF GA+ FA+QQGVE V + + IT  NVE L+LA EAN + +D      P      A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYAC 240
           E PL      IG     TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKG 300
           ++C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGFATEDGFMEVGIW 323
           E+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of PI0015029 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.5e-98
Identity = 193/327 (59.02%), Postives = 235/327 (71.87%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +GI AL++ +  +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  NDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           N   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NPISLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSET----CSASV 180
           LAF GA+   +QQGVE V + +FITE NVE L+LA EAN + +D    + T      A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYAC 240
           E PL      IG     TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKG 300
           ++C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGFATEDGFMEVGIW 323
           E+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

BLAST of PI0015029 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 360.5 bits (924), Expect = 1.9e-98
Identity = 191/323 (59.13%), Postives = 228/323 (70.59%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP  R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  NDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           N P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT  N+  L+ AKE N + LD  VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCG 240
                 IG     TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+ GEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGFATEDGFMEVGIW 323
            FNTTGMFR  A+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of PI0015029 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 309/322 (95.96%), Postives = 312/322 (96.89%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELEN
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELV NDYFITEGNVELLQLAKEANGIWLDRRVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MN VPI VYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIGLGTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG AGLIAVSS GEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRGFATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of PI0015029 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 309/322 (95.96%), Postives = 312/322 (96.89%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELEN
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELV NDYFITEGNVELLQLAKEANGIWLDRRVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MN VPI VYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIGLGTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG AGLIAVSS GEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRGFATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of PI0015029 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.7e-167
Identity = 294/322 (91.30%), Postives = 306/322 (95.03%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL CNDYF+TE NV +L+LA+EAN IWLD RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MNG+PI VYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG+AGLIAVSS GEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of PI0015029 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.0e-166
Identity = 292/322 (90.68%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL CNDYF+TE NV +L+LA+EAN IWLD RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MNG+PI VYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG+AGLIAVSS GEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of PI0015029 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.7e-159
Identity = 283/325 (87.08%), Postives = 303/325 (93.23%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           M GWAIAVHGGAGVDPNLPL RQ++AKKLL RCL+LGI ALRS+H AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPS---ETCSASVET 180
           AFSGAE+FARQQGVE+VCNDYFITE NV +L+LAKEAN I  D R+P+   E CSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDV 240
           PLQMNG+PI VYAPETVGCVVVDS+GRCAAATSTGGL+NK+ GRIGDSPLIG GTYAC +
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEG+AGLIAVSS GEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGFATEDGFMEVGIW 323
           ACGFNTTGMFRG ATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

BLAST of PI0015029 vs. NCBI nr
Match: XP_004141273.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis sativus])

HSP 1 Score: 627.9 bits (1618), Expect = 4.9e-176
Identity = 312/323 (96.59%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDP+LPLHRQDDAKK LTRCLDLGIHALRSNHSAIDVVELVVRELEN
Sbjct: 1   MGGWAIAVHGGAGVDPDLPLHRQDDAKKFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVET-PL 180
           AFSGAEKFARQQGVELV NDYFITEGNVELLQLAKEANGIWLDRRVP ETC ASVET PL
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPIETCCASVETPPL 180

Query: 181 QMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCG 240
           QMNGVPI VY PETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIGLGTYACDVCG
Sbjct: 181 QMNGVPISVYTPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVAC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEGQAGLIAVSSKGEVAC
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGQAGLIAVSSKGEVAC 300

Query: 301 GFNTTGMFRGFATEDGFMEVGIW 323
           GFNTTGMFRGFATEDGFMEVGIW
Sbjct: 301 GFNTTGMFRGFATEDGFMEVGIW 323

BLAST of PI0015029 vs. NCBI nr
Match: XP_008452604.1 (PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA0064417.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa] >TYK20170.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 625.5 bits (1612), Expect = 2.5e-175
Identity = 309/322 (95.96%), Postives = 312/322 (96.89%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELEN
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELV NDYFITEGNVELLQLAKEANGIWLDRRVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MN VPI VYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIGLGTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG AGLIAVSS GEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRGFATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

BLAST of PI0015029 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 3.7e-171
Identity = 303/322 (94.10%), Postives = 311/322 (96.58%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPLHRQ DAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELV NDYFITE NV LL+LAKEAN IWLD RVPSETCSASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MNG+ I VYAPETVGCVVVD+EGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG+AGLIAVSS GEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of PI0015029 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 294/322 (91.30%), Postives = 306/322 (95.03%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL CNDYF+TE NV +L+LA+EAN IWLD RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MNG+PI VYAPETVGCVVVDSEGRCAAATSTGGL+NKKIGRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG+AGLIAVSS GEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of PI0015029 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.6 bits (1529), Expect = 1.0e-165
Identity = 292/322 (90.68%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL CNDYF+TE NV +L+LA+EAN IWLD RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGV 240
           MNG+PI VYAPETVGCVVVDS+GRCAAATSTGGL+NKKIGRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYV+EERLDEG+AGLIAVSS GEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGFATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

BLAST of PI0015029 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 517.7 bits (1332), Expect = 6.7e-147
Identity = 254/324 (78.40%), Postives = 287/324 (88.58%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60
           MGGWAIAVHGGAG+DPNLP  RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELE 
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETC--SASVETP 180
           AFSGAE FAR+QGVE+V N+YF+T+ NV +L+LAKEAN I  D R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVC 240
           +QMNG+PI +YAPETVGCVVVD +G CAA TSTGGL+NK +GRIGDSPLIG GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YV++ RLDEG AGLIAVS+KGEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGFATEDGFMEVGIW 323
           CGFN+ GMFRG ATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

BLAST of PI0015029 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 360.5 bits (924), Expect = 1.4e-99
Identity = 191/323 (59.13%), Postives = 228/323 (70.59%), Query Frame = 0

Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP  R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  NDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           N P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVCNDYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT  N+  L+ AKE N + LD  VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCG 240
                 IG     TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+ GEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGFATEDGFMEVGIW 323
            FNTTGMFR  A+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

BLAST of PI0015029 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-73
Identity = 143/243 (58.85%), Postives = 172/243 (70.78%), Query Frame = 0

Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFARQQGVELVCN 139
           MEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH YLAF  AE FAR  GVE V +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFITEGNVELLQLAKEANGIWLDRRVPSETCSASVETPLQMNGVPIGVYAPETVGCVVV 199
            +FIT  N+  L+ AKE N + LD  VPS       + P       IG     TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSEGRCAAATSTGGLINKKIGRIGDSPLIGLGTYACDVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVMEERLDEGQAGLIAVSSKGEVACGFNTTGMFRGFATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+ GEV   FNTTGMFR  A+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

BLAST of PI0015029 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.0e-21
Identity = 87/315 (27.62%), Postives = 132/315 (41.90%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLHRQDDAKKLLT----RCLDLGIHALRSNHSAIDVVELVVRELEND 65
           +AVH GAG       H   + K L +     CL       + +   ID V   ++ LE+D
Sbjct: 15  VAVHVGAG------YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDD 74

Query: 66  PLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARL----------V 125
           P  N+GRGS LTEDG VE +AS+MDG     G V  +  V+N I +A L          +
Sbjct: 75  PSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTL 134

Query: 126 MDKSPHSYLAFSGAEKFARQQ-----GVELVCNDYFITE----------------GNVEL 185
           + + P   L   GA ++ + +     G     + + +TE                G   +
Sbjct: 135 LGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSI 194

Query: 186 LQLAKEANGIWLDRRVPSETCSASVETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATS 245
           L   +   G        +ETC  +V +    +   I     +TVG + VD+EG  A  +S
Sbjct: 195 LSAEEHPRG-----TENNETCEENVSSCAAADEDKI----MDTVGVICVDNEGHIACGSS 254

Query: 246 TGGLINKKIGRIGDSPLIGLGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVM 278
           +GG+  K  GR+G +   G G +A          + G   +G GE ++RG  ARE    +
Sbjct: 255 SGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSL 314

BLAST of PI0015029 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 90.5 bits (223), Expect = 2.6e-18
Identity = 85/307 (27.69%), Postives = 131/307 (42.67%), Query Frame = 0

Query: 6   IAVHGGAGVDPNLPLHRQDDAKKLLT----RCLDLGIHALRSNHSAIDVVELVVRELEND 65
           +AVH GAG       H   + K L +     CL       + +   ID V   ++ LE+D
Sbjct: 15  VAVHVGAG------YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDD 74

Query: 66  PLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPIS--LARLVMDKSPHSY 125
           P  N+GRGS LTEDG VE +AS+MDG     G   G+  V + +S  L++L++       
Sbjct: 75  PSTNAGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWL 134

Query: 126 LAFSGAEKFARQQ-----GVELVCNDYFITE----------------GNVELLQLAKEAN 185
           L   GA ++ + +     G     + + +TE                G   +L   +   
Sbjct: 135 LVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPR 194

Query: 186 GIWLDRRVPSETCSASVETPLQMNGVPIGVYAPETVGCVVVDSEGRCAAATSTGGLINKK 245
           G        +ETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K 
Sbjct: 195 G-----TENNETCEENVSSCAAADEDKI----MDTVGVICVDNEGHIACGSSSGGIAMKI 254

Query: 246 IGRIGDSPLIGLGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQ 278
            GR+G +   G G +A          + G   +G GE ++RG  ARE    +     G  
Sbjct: 255 SGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPA 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXG19.5e-14678.40Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ... [more]
P303641.6e-10059.76Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
Q9ZSD61.1e-9859.02Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502881.5e-9859.02Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
P502871.9e-9859.13Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
Match NameE-valueIdentityDescription
A0A5D3D9K21.2e-17595.96Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BV131.2e-17595.96probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1IYV77.7e-16791.30probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FND25.0e-16690.68probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1CHT17.7e-15987.08probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_004141273.14.9e-17696.59probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis sativus][more]
XP_008452604.12.5e-17595.96PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA00... [more]
XP_038896168.13.7e-17194.10probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida][more]
XP_022982271.11.6e-16691.30probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima][more]
XP_022941449.11.0e-16590.68probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447... [more]
Match NameE-valueIdentityDescription
AT3G16150.16.7e-14778.40N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.11.4e-9959.13N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.22.9e-7358.85N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.12.0e-2127.62N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.22.6e-1827.69N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 4..322
e-value: 2.3E-102
score: 342.2
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..322
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 193..322
e-value: 3.3E-43
score: 149.0
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 3..317
e-value: 2.8E-92
score: 307.1
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 3..317
e-value: 2.8E-92
score: 307.1
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 3..317
e-value: 2.8E-92
score: 307.1
NoneNo IPR availablePANTHERPTHR10188:SF36PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATEDcoord: 1..322
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 5..315
e-value: 3.07896E-122
score: 349.838
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 4..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015029.1PI0015029.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity