PI0015011 (gene) Melon (PI 482460) v1

Overview
NamePI0015011
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionervatamin-B-like
Locationchr02: 3347028 .. 3348394 (+)
RNA-Seq ExpressionPI0015011
SyntenyPI0015011
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCATCATCAAATTTCTTATTCTTTTCATTCCTCTATCTTCTATAGCACTTTCTGAGAGTTTTAAATTAGAAACAAAGGATTTTGAATCTGAAAGAAGTCTATGGCTCTTGTACAAAAGATGGAGTAGCTACCATAGAATCTCAAGAAATCCAAAGGAGATGCGCAACCGTTTCAAGGTATTCAAACACAATGCAAAGTTTGTGTTCCAAGAGAACCAAAAGAACAAATCATTAAAATTGAAGTTGAACCATTTCTCTGATTTGTCCGATGATGAGTTTATGAACCTTCATCTTAATGTCAATCTTACTTACTACAAAAACTTAAACGCTAAGAACATCGAAGCCAATGGTGGCGGTGGTTTTATTGGCGGGTTTAGGTATGAGAATGCTGAGGATCTTCCGGCTTCAATTGATTGGAGGAAAAAAGGAGCTGTGAATAAAATAAAAAACCAAGGCGGATGTGGTAATTAAATTCTAAACCTAATTATGGTAATTAAATTCTAAACCTAATTATTTTAAGCTTGTGTGTGTTTGTTATAACTAAATGAAATAATATTAATAATTATAATTATGTTTGCAGGAAGTTGTTGGGCGTTTGCAGCTGTCGCTGTGGTGGAATCGATTCACCAAATAAAAACAAAGAAGTTAGTATCTCTATCTGAGCAACAAGTGGTGGATTGTGATTATAGGGACGGAGGTTGTCGTGGAGGATTCTATAACTCTGCTTTTGAATTCATAATGGAAAACAACGGGATCACACTCGAAGATAACTATCCCTATTATTTTGGCAATGGATATTGCAGACGAGGAGTGAGTCTTGAGTTTATATGAACCATATACATAATTCTAATTAAACTTATGGTCATTGCATGCATTCATAATAATTAATTATGTGTTTGTGTTTGTGTTTGATTTAACAACAAATAGAGCAATAATAAGGTGACAATTGATGGATATGAGAATGTATCTCCATTCAATGAGAATGCTCTAAAGAAAGCCGTGGCACACCAACCTGTAGCCGTGTCCCTAGCCTCCAATGGACTACATTTTCGTCATTACGCTGAGGCAAGTTTAATTTAGTTTTTTTTTAAAAAAAAATTGAACTCCAATGGAGAATTATTATTTTAAAAGAGAAATTGTAAATTATTTTGCAGGGAATGTTCACGGAGGATGACTGTCGAATTGATCACACAGTGGCCGTAGTTGGATACGGAACGGATGAAGACGGAGATTATTGGATCATAAGGAACCAATGGGGAACTGGATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCTAAGTCACCACGAGGTGTATGTGGAATGGCAATAGAACCAGCCTATCCAGTAAAGAACTAA

mRNA sequence

ATGTCCATCATCAAATTTCTTATTCTTTTCATTCCTCTATCTTCTATAGCACTTTCTGAGAGTTTTAAATTAGAAACAAAGGATTTTGAATCTGAAAGAAGTCTATGGCTCTTGTACAAAAGATGGAGTAGCTACCATAGAATCTCAAGAAATCCAAAGGAGATGCGCAACCGTTTCAAGGTATTCAAACACAATGCAAAGTTTGTGTTCCAAGAGAACCAAAAGAACAAATCATTAAAATTGAAGTTGAACCATTTCTCTGATTTGTCCGATGATGAGTTTATGAACCTTCATCTTAATGTCAATCTTACTTACTACAAAAACTTAAACGCTAAGAACATCGAAGCCAATGGTGGCGGTGGTTTTATTGGCGGGTTTAGGTATGAGAATGCTGAGGATCTTCCGGCTTCAATTGATTGGAGGAAAAAAGGAGCTGTGAATAAAATAAAAAACCAAGGCGGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTCGCTGTGGTGGAATCGATTCACCAAATAAAAACAAAGAAGTTAGTATCTCTATCTGAGCAACAAGTGGTGGATTGTGATTATAGGGACGGAGGTTGTCGTGGAGGATTCTATAACTCTGCTTTTGAATTCATAATGGAAAACAACGGGATCACACTCGAAGATAACTATCCCTATTATTTTGGCAATGGATATTGCAGACGAGGAAGCAATAATAAGGTGACAATTGATGGATATGAGAATGTATCTCCATTCAATGAGAATGCTCTAAAGAAAGCCGTGGCACACCAACCTGTAGCCGTGTCCCTAGCCTCCAATGGACTACATTTTCGTCATTACGCTGAGGGAATGTTCACGGAGGATGACTGTCGAATTGATCACACAGTGGCCGTAGTTGGATACGGAACGGATGAAGACGGAGATTATTGGATCATAAGGAACCAATGGGGAACTGGATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCTAAGTCACCACGAGGTGTATGTGGAATGGCAATAGAACCAGCCTATCCAGTAAAGAACTAA

Coding sequence (CDS)

ATGTCCATCATCAAATTTCTTATTCTTTTCATTCCTCTATCTTCTATAGCACTTTCTGAGAGTTTTAAATTAGAAACAAAGGATTTTGAATCTGAAAGAAGTCTATGGCTCTTGTACAAAAGATGGAGTAGCTACCATAGAATCTCAAGAAATCCAAAGGAGATGCGCAACCGTTTCAAGGTATTCAAACACAATGCAAAGTTTGTGTTCCAAGAGAACCAAAAGAACAAATCATTAAAATTGAAGTTGAACCATTTCTCTGATTTGTCCGATGATGAGTTTATGAACCTTCATCTTAATGTCAATCTTACTTACTACAAAAACTTAAACGCTAAGAACATCGAAGCCAATGGTGGCGGTGGTTTTATTGGCGGGTTTAGGTATGAGAATGCTGAGGATCTTCCGGCTTCAATTGATTGGAGGAAAAAAGGAGCTGTGAATAAAATAAAAAACCAAGGCGGATGTGGAAGTTGTTGGGCGTTTGCAGCTGTCGCTGTGGTGGAATCGATTCACCAAATAAAAACAAAGAAGTTAGTATCTCTATCTGAGCAACAAGTGGTGGATTGTGATTATAGGGACGGAGGTTGTCGTGGAGGATTCTATAACTCTGCTTTTGAATTCATAATGGAAAACAACGGGATCACACTCGAAGATAACTATCCCTATTATTTTGGCAATGGATATTGCAGACGAGGAAGCAATAATAAGGTGACAATTGATGGATATGAGAATGTATCTCCATTCAATGAGAATGCTCTAAAGAAAGCCGTGGCACACCAACCTGTAGCCGTGTCCCTAGCCTCCAATGGACTACATTTTCGTCATTACGCTGAGGGAATGTTCACGGAGGATGACTGTCGAATTGATCACACAGTGGCCGTAGTTGGATACGGAACGGATGAAGACGGAGATTATTGGATCATAAGGAACCAATGGGGAACTGGATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAGCTAAGTCACCACGAGGTGTATGTGGAATGGCAATAGAACCAGCCTATCCAGTAAAGAACTAA

Protein sequence

MSIIKFLILFIPLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNNKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDDCRIDHTVAVVGYGTDEDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN
Homology
BLAST of PI0015011 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.7e-87
Identity = 168/348 (48.28%), Postives = 230/348 (66.09%), Query Frame = 0

Query: 5   KFLILFIPLSSI-ALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFK 64
           KF++L + L+ + A++ESF    K+ ESE SLW LY+RW S+H +SR+  E + RF VFK
Sbjct: 3   KFILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNVFK 62

Query: 65  HNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFI 124
           HNA  V   N+ +K  KLKLN F+D+++ EF N +    + +++          GG    
Sbjct: 63  HNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMF-------RGGPRGN 122

Query: 125 GGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSE 184
           G F YE  + +PAS+DWRKKGAV  +K+QG CGSCWAF+ +  VE I+QIKT KLVSLSE
Sbjct: 123 GTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSE 182

Query: 185 QQVVDCDY-RDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNN--KVTID 244
           Q++VDCD  ++ GC GG  + AFEFI +  GIT E NYPY   +G C     N   V+ID
Sbjct: 183 QELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSID 242

Query: 245 GYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTED-DCRIDHTVAVVGYGT 304
           G+ENV   +ENAL KAVA+QPV+V++ + G  F+ Y+EG+FT      +DH VA+VGYGT
Sbjct: 243 GHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGT 302

Query: 305 DEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
             DG  YW ++N WG  WG  GY++M+RG     G+CG+A+E +YP+K
Sbjct: 303 TIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of PI0015011 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.7e-85
Identity = 170/354 (48.02%), Postives = 226/354 (63.84%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSI-ALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRF 60
           M++ K L + + LS +  ++ SF    KD ESE SLW LY+RW S+H +SR+  E   RF
Sbjct: 1   MAMKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  KVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGG 120
            VFK N   V   N+ +K  KLKLN F+D+++ EF + +    + ++K         +G 
Sbjct: 61  NVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQ---HGS 120

Query: 121 GGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLV 180
           G F+    YE    +PAS+DWRKKGAV  +K+QG CGSCWAF+ +  VE I+QIKT KLV
Sbjct: 121 GTFM----YEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLV 180

Query: 181 SLSEQQVVDCDYRDG-GCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNN--K 240
           SLSEQ++VDCD  +  GC GG   SAFEFI +  GIT E NYPY    G C     N   
Sbjct: 181 SLSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLA 240

Query: 241 VTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDDCRID--HTVAV 300
           V+IDG+ENV   +ENAL KAVA+QPV+V++ + G  F+ Y+EG+FT  DC  D  H VA+
Sbjct: 241 VSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT-GDCNTDLNHGVAI 300

Query: 301 VGYGTDEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           VGYGT  DG +YWI+RN WG  WG  GY++MQR      G+CG+A+  +YP+KN
Sbjct: 301 VGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

BLAST of PI0015011 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 310.1 bits (793), Expect = 3.2e-83
Identity = 162/338 (47.93%), Postives = 214/338 (63.31%), Query Frame = 0

Query: 16  IALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFKHNAKFVFQENQK 75
           + ++ SF    KD  SE SLW LY+RW S+H +SR+  E   RF VFK N   V   N+ 
Sbjct: 17  LGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKANLMHVHNTNKM 76

Query: 76  NKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFIGGFRYENAEDLP 135
           +K  KLKLN F+D+++ EF + +    + + +       E        G F YE    +P
Sbjct: 77  DKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHEN-------GAFMYEKVVSVP 136

Query: 136 ASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSEQQVVDCDYRDG- 195
            S+DWRKKGAV  +K+QG CGSCWAF+ V  VE I+QIKT KLV+LSEQ++VDCD  +  
Sbjct: 137 PSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDKEENQ 196

Query: 196 GCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNN--KVTIDGYENVSPFNENA 255
           GC GG   SAFEFI +  GIT E NYPY    G C     N   V+IDG+ENV   +E+A
Sbjct: 197 GCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDA 256

Query: 256 LKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDDCRID--HTVAVVGYGTDEDG-DYWIIR 315
           L KAVA+QPV+V++ + G  F+ Y+EG+FT  DC  D  H VA+VGYGT  DG +YWI+R
Sbjct: 257 LLKAVANQPVSVAIDAGGSDFQFYSEGVFT-GDCSTDLNHGVAIVGYGTTVDGTNYWIVR 316

Query: 316 NQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           N WG  WG +GY++MQR      G+CG+A+ P+YP+KN
Sbjct: 317 NSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKN 346

BLAST of PI0015011 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.2e-83
Identity = 164/347 (47.26%), Postives = 224/347 (64.55%), Query Frame = 0

Query: 5   KFLILFI-PLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFK 64
           K L++F+  L  +  +  F  + K+ ESE  L  LY RW S+H + R+  E   RF VF+
Sbjct: 3   KLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVFR 62

Query: 65  HNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFI 124
           HN   V   N+KN+S KLKLN F+DL+ +EF N +   N+ +++ L        G   F+
Sbjct: 63  HNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK---RGSKQFM 122

Query: 125 GGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSE 184
             + +EN   LP+S+DWRKKGAV +IKNQG CGSCWAF+ VA VE I++IKT KLVSLSE
Sbjct: 123 --YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSE 182

Query: 185 QQVVDCDYRDG-GCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNN--KVTID 244
           Q++VDCD +   GC GG    AFEFI +N GIT ED+YPY   +G C    +N   VTID
Sbjct: 183 QELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTID 242

Query: 245 GYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTED-DCRIDHTVAVVGYGT 304
           G+E+V   +ENAL KAVA+QPV+V++ +    F+ Y+EG+FT      ++H VA VGYG+
Sbjct: 243 GHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGS 302

Query: 305 DEDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           +    YWI+RN WG  WG  GY+K++R    P G CG+A+E +YP+K
Sbjct: 303 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of PI0015011 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.1e-82
Identity = 159/349 (45.56%), Postives = 222/349 (63.61%), Query Frame = 0

Query: 5   KFLILFI-PLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFK 64
           +F++L +  L  +  ++      KD ESE SLW LY+RW S+H ++R+ +E   RF VFK
Sbjct: 3   RFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNVFK 62

Query: 65  HNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFI 124
           HN K + + N+K+KS KLKLN F D++ +EF   +   N+ +++    +           
Sbjct: 63  HNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATK------ 122

Query: 125 GGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSE 184
             F Y N   LP S+DWRK GAV  +KNQG CGSCWAF+ V  VE I+QI+TKKL SLSE
Sbjct: 123 -SFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSE 182

Query: 185 QQVVDCD-YRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNNK--VTID 244
           Q++VDCD  ++ GC GG  + AFEFI E  G+T E  YPY   +  C     N   V+ID
Sbjct: 183 QELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSID 242

Query: 245 GYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFT-EDDCRIDHTVAVVGYGT 304
           G+E+V   +E+ L KAVA+QPV+V++ + G  F+ Y+EG+FT      ++H VAVVGYGT
Sbjct: 243 GHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGT 302

Query: 305 DEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
             DG  YWI++N WG  WG  GY++MQRG +   G+CG+A+E +YP+KN
Sbjct: 303 TIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLKN 344

BLAST of PI0015011 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.1e-138
Identity = 254/355 (71.55%), Postives = 294/355 (82.82%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIALS----ESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  +A S    ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MTVMKFLI--VPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
           NRFKVFK+NAK VF+ N   KSLKLKLN F+D+SDDEF N++ + N+TYYK+L+AK IEA
Sbjct: 61  NRFKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKKIEA 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
            GG   IGGF YE+A ++P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 TGGR--IGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSE++V+DCDYRDGGCRGGFYNSAFEF+M+N+G+T+EDNYPYY GNGYCRR  G N
Sbjct: 181 ELVSLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHT 300
            +V IDGYENV   NE AL KAVAHQPVAV++AS G  F+ Y  GMFTE+D     IDHT
Sbjct: 241 KRVRIDGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHT 300

Query: 301 VAVVGYGTDEDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           V VVGYGTDEDGDYWIIRNQ+G  WGMNGYMKMQRGA SP+GVCGMA++PAYPVK
Sbjct: 301 VVVVGYGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVK 350

BLAST of PI0015011 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.5e-136
Identity = 254/357 (71.15%), Postives = 293/357 (82.07%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIA----LSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  IA    L ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MAVMKFLI--VPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
            RFKVFK NAK+VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AKN   
Sbjct: 61  KRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN--- 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
               G +GGF YE+A D+P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 ----GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSEQ+VVDCDYRD GC GGFYNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N
Sbjct: 181 ELVSLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHT 300
            +VTIDGYENV   NE+AL KAVAHQPVAV++AS+G  FR Y +GMFTE D     IDHT
Sbjct: 241 ERVTIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHT 300

Query: 301 VAVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           V VVGYGTD EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMAI+PAYPVK+
Sbjct: 301 VVVVGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of PI0015011 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.9e-135
Identity = 254/359 (70.75%), Postives = 295/359 (82.17%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIA----LSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  IA    L ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MAVMKFLI--VPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
            RFKVFK NAK+VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AKN   
Sbjct: 61  KRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN--- 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
               G +GGF YE+A D+P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 ----GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSEQ+VVDCDYRD GC GGFYNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N
Sbjct: 181 ELVSLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNG--LHFRHYAEGMFTEDD---CRID 300
            +VTIDGYENV   NE+AL KAVAHQPVAV++AS+G  L+F +Y +GMFTE D     ID
Sbjct: 241 ERVTIDGYENVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNID 300

Query: 301 HTVAVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           HTV VVGYGTD EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMAI+PAYPVK+
Sbjct: 301 HTVVVVGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

BLAST of PI0015011 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 4.9e-135
Identity = 250/355 (70.42%), Postives = 292/355 (82.25%), Query Frame = 0

Query: 2   SIIKFLILFIPLSSIALS----ESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRN 61
           +++KFLI  +PL  IAL+    ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM  
Sbjct: 3   TVMKFLI--VPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHK 62

Query: 62  RFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEAN 121
           RFKVFK NAK VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AK     
Sbjct: 63  RFKVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT---- 122

Query: 122 GGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKK 181
              G +GGF YE+A+++P+SIDWRKKGAVN IKNQGGCGSCWAFAAVA VESIHQIKT +
Sbjct: 123 ---GHVGGFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNE 182

Query: 182 LVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSNN 241
           LVSLSEQ+VVDCDYRDGGCRGG YNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N 
Sbjct: 183 LVSLSEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNE 242

Query: 242 KVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHTV 301
           +V IDGYENV   NE+AL KAVAHQPVAV++AS+G  FR Y +GMFTE D     IDHTV
Sbjct: 243 RVKIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTV 302

Query: 302 AVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
            VVGYG+D EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMA++PAYPVK
Sbjct: 303 VVVGYGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVK 347

BLAST of PI0015011 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.4e-134
Identity = 249/355 (70.14%), Postives = 291/355 (81.97%), Query Frame = 0

Query: 2   SIIKFLILFIPLSSIALS----ESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRN 61
           +++KFLI  +P   IAL+    ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM  
Sbjct: 3   TVMKFLI--VPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHK 62

Query: 62  RFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEAN 121
           RFKVFK NAK VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AK     
Sbjct: 63  RFKVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT---- 122

Query: 122 GGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKK 181
              G +GGF YE+A+++P+SIDWRKKGAVN IKNQGGCGSCWAFAAVA VESIHQIKT +
Sbjct: 123 ---GHVGGFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNE 182

Query: 182 LVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSNN 241
           LVSLSEQ+VVDCDYRDGGCRGG YNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N 
Sbjct: 183 LVSLSEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNE 242

Query: 242 KVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHTV 301
           +V IDGYENV   NE+AL KAVAHQPVAV++AS+G  FR Y +GMFTE D     IDHTV
Sbjct: 243 RVKIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTV 302

Query: 302 AVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
            VVGYG+D EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMA++PAYPVK
Sbjct: 303 VVVGYGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVK 347

BLAST of PI0015011 vs. NCBI nr
Match: XP_038896226.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 503.4 bits (1295), Expect = 1.5e-138
Identity = 251/352 (71.31%), Postives = 294/352 (83.52%), Query Frame = 0

Query: 2   SIIKFLI--LFIPLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRF 61
           +I+KFLI  L + + +  L ESFK E K+ ESE SLW LYKRWSS+HRI RN +EM NRF
Sbjct: 43  AIMKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRF 102

Query: 62  KVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGG 121
           KVFK NAK+VF+ NQ NKSLKLKLN F+D+SDDEF+N H++ N+TYYKNL+AK IEA GG
Sbjct: 103 KVFKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGG 162

Query: 122 GGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLV 181
              +GGF YE+ EDLP+SIDWRKKGAV  IKNQG CGSCWAFAAVAVVE I+QIKT KLV
Sbjct: 163 R--VGGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLV 222

Query: 182 SLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYC-RRGSNNKVT 241
           SLSEQ+VVDCDY+DGGC GGFY+SAFEF+MENNGIT+E+NYPYY  N YC  +  N +VT
Sbjct: 223 SLSEQEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNKRVT 282

Query: 242 IDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD-C--RIDHTVAVV 301
           IDGYENV P NENALKKAVAHQPVAVS+A++G  FR+Y+EGMFTE+D C  RIDHTV VV
Sbjct: 283 IDGYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEGMFTENDYCGDRIDHTVVVV 342

Query: 302 GYGTDEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           GYGT+EDG DYWII+N WGT WG+ GYMKMQRGA+ P GVCG+A+ P+YP+K
Sbjct: 343 GYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 392

BLAST of PI0015011 vs. NCBI nr
Match: XP_011658479.1 (ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.7e-137
Identity = 254/355 (71.55%), Postives = 294/355 (82.82%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIALS----ESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  +A S    ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MTVMKFLI--VPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
           NRFKVFK+NAK VF+ N   KSLKLKLN F+D+SDDEF N++ + N+TYYK+L+AK IEA
Sbjct: 61  NRFKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKKIEA 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
            GG   IGGF YE+A ++P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 TGGR--IGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSE++V+DCDYRDGGCRGGFYNSAFEF+M+N+G+T+EDNYPYY GNGYCRR  G N
Sbjct: 181 ELVSLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHT 300
            +V IDGYENV   NE AL KAVAHQPVAV++AS G  F+ Y  GMFTE+D     IDHT
Sbjct: 241 KRVRIDGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHT 300

Query: 301 VAVVGYGTDEDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           V VVGYGTDEDGDYWIIRNQ+G  WGMNGYMKMQRGA SP+GVCGMA++PAYPVK
Sbjct: 301 VVVVGYGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVK 350

BLAST of PI0015011 vs. NCBI nr
Match: XP_038885798.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 498.8 bits (1283), Expect = 3.7e-137
Identity = 253/355 (71.27%), Postives = 294/355 (82.82%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIA----LSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           ++++KFLI  +PL  IA    L ESF  E  + ESE SLW LYKRWSS+HRISRN  EM 
Sbjct: 66  ITMMKFLI--VPLVLIALIFGLCESFTFEGNELESEESLWHLYKRWSSHHRISRNGWEMH 125

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
            RFKVFK NAK+VF+ NQ NKSLKLKLN F+D+SDDEF+N H+N N+TYYKNL+AK  EA
Sbjct: 126 YRFKVFKDNAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVNSNITYYKNLHAKKKEA 185

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
            GGG  +GGF YE+AEDLP+SIDWRKKGAV  IKNQGGCGSCWAFAAVAVVE IHQIKT 
Sbjct: 186 VGGGR-VGGFMYEHAEDLPSSIDWRKKGAVTDIKNQGGCGSCWAFAAVAVVEGIHQIKTN 245

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYC-RRGSNN 240
           KLVSLSEQ+VVDCDYRDGGC GGFY+SAFEF+M+NNGIT E+NYPYY  N YC  + +N 
Sbjct: 246 KLVSLSEQEVVDCDYRDGGCGGGFYDSAFEFMMKNNGITTEENYPYYAENRYCLSQRANK 305

Query: 241 KVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD-C--RIDHTV 300
           +VTIDGYENV P NENALKKAVA+QPVAVS+A++G  FR+Y+EGMFTE+D C  RIDHTV
Sbjct: 306 RVTIDGYENVPPNNENALKKAVANQPVAVSIAASGRRFRYYSEGMFTENDYCGDRIDHTV 365

Query: 301 AVVGYGTDEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
            VVGYGT+EDG DYWII+N WGT WG+ GYMKMQRGA+ P  VCG+A+ P+YP+K
Sbjct: 366 VVVGYGTEEDGADYWIIKNSWGTNWGLEGYMKMQRGARQPEDVCGLAMNPSYPIK 417

BLAST of PI0015011 vs. NCBI nr
Match: XP_008454482.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 495.7 bits (1275), Expect = 3.2e-136
Identity = 254/357 (71.15%), Postives = 293/357 (82.07%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIA----LSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  IA    L ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MAVMKFLI--VPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
            RFKVFK NAK+VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AKN   
Sbjct: 61  KRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN--- 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
               G +GGF YE+A D+P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 ----GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSEQ+VVDCDYRD GC GGFYNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N
Sbjct: 181 ELVSLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDD---CRIDHT 300
            +VTIDGYENV   NE+AL KAVAHQPVAV++AS+G  FR Y +GMFTE D     IDHT
Sbjct: 241 ERVTIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHT 300

Query: 301 VAVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           V VVGYGTD EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMAI+PAYPVK+
Sbjct: 301 VVVVGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of PI0015011 vs. NCBI nr
Match: TYK16990.1 (ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 491.5 bits (1264), Expect = 6.0e-135
Identity = 254/359 (70.75%), Postives = 295/359 (82.17%), Query Frame = 0

Query: 1   MSIIKFLILFIPLSSIA----LSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMR 60
           M+++KFLI  +PL  IA    L ESF+LE KDFESE+SL  LYKRWSS+HRISRN  EM 
Sbjct: 1   MAVMKFLI--VPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMH 60

Query: 61  NRFKVFKHNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEA 120
            RFKVFK NAK+VF++N   +SLKL+LN F+D+SDDEF ++H   N+TYYKNL+AKN   
Sbjct: 61  KRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN--- 120

Query: 121 NGGGGFIGGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTK 180
               G +GGF YE+A D+P+SIDWRKKGAVN IKNQG CGSCWAFAAVA VESIHQIKT 
Sbjct: 121 ----GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTN 180

Query: 181 KLVSLSEQQVVDCDYRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRR--GSN 240
           +LVSLSEQ+VVDCDYRD GC GGFYNSAFEF+MEN GIT+EDNYPYY G+GYCRR  G N
Sbjct: 181 ELVSLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYN 240

Query: 241 NKVTIDGYENVSPFNENALKKAVAHQPVAVSLASNG--LHFRHYAEGMFTEDD---CRID 300
            +VTIDGYENV   NE+AL KAVAHQPVAV++AS+G  L+F +Y +GMFTE D     ID
Sbjct: 241 ERVTIDGYENVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNID 300

Query: 301 HTVAVVGYGTD-EDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
           HTV VVGYGTD EDGDYWIIRNQ+GT WGMNGYMKMQRGA++P+GVCGMAI+PAYPVK+
Sbjct: 301 HTVVVVGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

BLAST of PI0015011 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.0e-84
Identity = 164/347 (47.26%), Postives = 224/347 (64.55%), Query Frame = 0

Query: 5   KFLILFI-PLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFK 64
           K L++F+  L  +  +  F  + K+ ESE  L  LY RW S+H + R+  E   RF VF+
Sbjct: 3   KLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVFR 62

Query: 65  HNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFI 124
           HN   V   N+KN+S KLKLN F+DL+ +EF N +   N+ +++ L        G   F+
Sbjct: 63  HNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK---RGSKQFM 122

Query: 125 GGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSE 184
             + +EN   LP+S+DWRKKGAV +IKNQG CGSCWAF+ VA VE I++IKT KLVSLSE
Sbjct: 123 --YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSE 182

Query: 185 QQVVDCDYRDG-GCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNN--KVTID 244
           Q++VDCD +   GC GG    AFEFI +N GIT ED+YPY   +G C    +N   VTID
Sbjct: 183 QELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTID 242

Query: 245 GYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTED-DCRIDHTVAVVGYGT 304
           G+E+V   +ENAL KAVA+QPV+V++ +    F+ Y+EG+FT      ++H VA VGYG+
Sbjct: 243 GHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGS 302

Query: 305 DEDGDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           +    YWI+RN WG  WG  GY+K++R    P G CG+A+E +YP+K
Sbjct: 303 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of PI0015011 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.5e-83
Identity = 159/349 (45.56%), Postives = 222/349 (63.61%), Query Frame = 0

Query: 5   KFLILFI-PLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFK 64
           +F++L +  L  +  ++      KD ESE SLW LY+RW S+H ++R+ +E   RF VFK
Sbjct: 3   RFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNVFK 62

Query: 65  HNAKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFI 124
           HN K + + N+K+KS KLKLN F D++ +EF   +   N+ +++    +           
Sbjct: 63  HNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATK------ 122

Query: 125 GGFRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSE 184
             F Y N   LP S+DWRK GAV  +KNQG CGSCWAF+ V  VE I+QI+TKKL SLSE
Sbjct: 123 -SFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSE 182

Query: 185 QQVVDCD-YRDGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNNK--VTID 244
           Q++VDCD  ++ GC GG  + AFEFI E  G+T E  YPY   +  C     N   V+ID
Sbjct: 183 QELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSID 242

Query: 245 GYENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFT-EDDCRIDHTVAVVGYGT 304
           G+E+V   +E+ L KAVA+QPV+V++ + G  F+ Y+EG+FT      ++H VAVVGYGT
Sbjct: 243 GHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGT 302

Query: 305 DEDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
             DG  YWI++N WG  WG  GY++MQRG +   G+CG+A+E +YP+KN
Sbjct: 303 TIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLKN 344

BLAST of PI0015011 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 9.0e-81
Identity = 155/347 (44.67%), Postives = 221/347 (63.69%), Query Frame = 0

Query: 6   FLILFIPLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPKEMRNRFKVFKHN 65
           F++L   LS +  S+ F  + K+ E+E ++W LY+RW  +H +SR   E   RF VF+HN
Sbjct: 5   FIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRHN 64

Query: 66  AKFVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFIGG 125
              V + N+KNK  KLK+N F+D++  EF + +   N+ +++ L        G GGF+  
Sbjct: 65  VLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPK---RGSGGFM-- 124

Query: 126 FRYENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSEQQ 185
             YEN   +P+S+DWR+KGAV ++KNQ  CGSCWAF+ VA VE I++I+T KLVSLSEQ+
Sbjct: 125 --YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQE 184

Query: 186 VVDCDYRDG-GCRGGFYNSAFEFIMENNGITLEDNYPYYFGN-GYCRRGS--NNKVTIDG 245
           +VDCD  +  GC GG    AFEFI  N GI  E+ YPY   +  +CR  S     VTIDG
Sbjct: 185 LVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDG 244

Query: 246 YENVSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFT-EDDCRIDHTVAVVGYGTD 305
           +E+V   +E  L KAVAHQPV+V++ +    F+ Y+EG+F  E   +++H V +VGYG  
Sbjct: 245 HEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGET 304

Query: 306 EDG-DYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
           ++G  YWI+RN WG  WG  GY++++RG     G CG+A+E +YP K
Sbjct: 305 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of PI0015011 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 273.1 bits (697), Expect = 3.1e-73
Identity = 147/345 (42.61%), Postives = 213/345 (61.74%), Query Frame = 0

Query: 11  IPLSSIALSESFKLETKDFESERSLWLLYKRWSSYHRISRNPK---EMRNRFKVFKHNAK 70
           + +S I+  E   + T    SE  +  +Y+ W   H  +++     E   RF++FK N +
Sbjct: 22  VDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLR 81

Query: 71  FVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFIGGFR 130
           FV + N+KN S +L L  F+DL++DE+ +          K L AK +E  G       + 
Sbjct: 82  FVDEHNEKNLSYRLGLTRFADLTNDEYRS----------KYLGAK-MEKKGERRTSLRYE 141

Query: 131 YENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSEQQVV 190
               ++LP SIDWRKKGAV ++K+QGGCGSCWAF+ +  VE I+QI T  L++LSEQ++V
Sbjct: 142 ARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQELV 201

Query: 191 DCDYR-DGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNNK--VTIDGYEN 250
           DCD   + GC GG  + AFEFI++N GI  + +YPY   +G C +   N   VTID YE+
Sbjct: 202 DCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDSYED 261

Query: 251 VSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDDC--RIDHTVAVVGYGTDED 310
           V  ++E +LKKAVAHQP+++++ + G  F+ Y  G+F +  C  ++DH V  VGYGT+  
Sbjct: 262 VPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIF-DGSCGTQLDHGVVAVGYGTENG 321

Query: 311 GDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVKN 348
            DYWI+RN WG  WG +GY++M R   S  G CG+AIEP+YP+KN
Sbjct: 322 KDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKN 354

BLAST of PI0015011 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.6e-69
Identity = 140/344 (40.70%), Postives = 211/344 (61.34%), Query Frame = 0

Query: 13  LSSIALSESFKLETKDFESERSLWLLYKRWSSYH---RISRN--PKEMRNRFKVFKHNAK 72
           +S I+  E+  + T+   S+  +  +Y+ W   H   ++++N    E   RF++FK N +
Sbjct: 24  MSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLR 83

Query: 73  FVFQENQKNKSLKLKLNHFSDLSDDEFMNLHLNVNLTYYKNLNAKNIEANGGGGFIGGFR 132
           F+ + N KN S KL L  F+DL+++E+ +++L    T      +   +A  G        
Sbjct: 84  FIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAKPTKRVLKTSDRYQARVG-------- 143

Query: 133 YENAEDLPASIDWRKKGAVNKIKNQGGCGSCWAFAAVAVVESIHQIKTKKLVSLSEQQVV 192
               + LP S+DWRK+GAV  +K+QG CGSCWAF+ +  VE I++I T  L+SLSEQ++V
Sbjct: 144 ----DALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELV 203

Query: 193 DCDYR-DGGCRGGFYNSAFEFIMENNGITLEDNYPYYFGNGYCRRGSNNK--VTIDGYEN 252
           DCD   + GC GG  + AFEFI++N GI  E +YPY   +G C +   N   VTID YE+
Sbjct: 204 DCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYED 263

Query: 253 VSPFNENALKKAVAHQPVAVSLASNGLHFRHYAEGMFTEDDC--RIDHTVAVVGYGTDED 312
           V   +E +LKKA+AHQP++V++ + G  F+ Y+ G+F +  C   +DH V  VGYGT+  
Sbjct: 264 VPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVF-DGLCGTELDHGVVAVGYGTENG 323

Query: 313 GDYWIIRNQWGTGWGMNGYMKMQRGAKSPRGVCGMAIEPAYPVK 347
            DYWI+RN WG  WG +GY+KM R  ++P G CG+A+E +YP+K
Sbjct: 324 KDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIK 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O650393.7e-8748.28Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
P124127.7e-8548.02Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
P258033.2e-8347.93Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9STL44.2e-8347.26KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Q9FGR92.1e-8245.56KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB18.1e-13871.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1[more]
A0A1S3BYU01.5e-13671.15ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1[more]
A0A5D3D0432.9e-13570.75Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001... [more]
A0A1S3BYQ34.9e-13570.42ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1[more]
A0A5A7TM641.4e-13470.14Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034... [more]
Match NameE-valueIdentityDescription
XP_038896226.11.5e-13871.31vignain-like [Benincasa hispida][more]
XP_011658479.11.7e-13771.55ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucum... [more]
XP_038885798.13.7e-13771.27vignain-like [Benincasa hispida][more]
XP_008454482.13.2e-13671.15PREDICTED: ervatamin-B-like [Cucumis melo][more]
TYK16990.16.0e-13570.75ervatamin-B-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G48340.13.0e-8447.26Cysteine proteinases superfamily protein [more]
AT5G50260.11.5e-8345.56Cysteine proteinases superfamily protein [more]
AT3G48350.19.0e-8144.67Cysteine proteinases superfamily protein [more]
AT1G47128.13.1e-7342.61Granulin repeat cysteine protease family protein [more]
AT5G43060.11.6e-6940.70Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 290..300
score: 53.76
coord: 305..311
score: 55.51
coord: 152..167
score: 59.3
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 134..345
e-value: 3.6E-97
score: 338.8
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 134..344
e-value: 4.7E-71
score: 239.3
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 39..94
e-value: 1.1E-8
score: 44.8
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 39..94
e-value: 4.5E-10
score: 39.8
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 14..347
e-value: 2.8E-103
score: 348.0
NoneNo IPR availablePANTHERPTHR12411:SF706KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1coord: 19..346
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 19..346
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 152..163
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 135..344
e-value: 1.56392E-98
score: 288.368
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 32..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015011.1PI0015011.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008234 cysteine-type peptidase activity