PI0014700 (gene) Melon (PI 482460) v1

Overview


Name	PI0014700
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Prolyl-tRNA synthetase
Location	chr11: 8384355 .. 8385679 (-)
RNA-Seq Expression	PI0014700
Synteny	PI0014700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTTTGAAAAGGTTACTATGTTTGTTGGGTGTATTCCAGTGAAGTGATGGTATGGTTGCCATGGTCATGGACTCATGGGCTTATGAATATCATTTCAATCTTCGTGATATTTTGTTGCATAAATTTTTTTTAAGAGTCAAAACTAGATTGCTTTTCTTTTTTAAGTTTGGAGATTGTTAATGAATTCTTTAGGAGGGTTTTTTTTTTAAATTTTTATCTTTTTATTTAAGTCACGTGCATTTTATTTTTGCCATGTGTTAGGTTTAATTGATTTTTCTTGCATGATGAACCGTGTTCCATTGTTCATATAGATGAACCAATGGGTAAAGCTGTCATGCTTCTGTTTTCTCTCTTTCTTTTTCTTTTTTGGTAGAAAACAAAGTTATATAAACAATTGGAAGAATATAAAGGGGAGAGGAAAGATTAAGTCTTCATCAAAATCTTAACTAAGCCACGGGAGATTACCAAAATACCCTTTAATCTAAGTCTGAAATTTTGCAATTCTTTTGTGTAACTGTGGTCATTATCTAAAGGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTGGCGATTCCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGTATAAGTTGACTAAATGCTATGACCCATCTTCAGCATGGCAAATTGATATTCATCACTTATTTAAGTTGTATAAAAGCATTGTTCCGTTTGTGATATGCAGGCATTTGTCCTAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAAGGGGAGAAAGCTATGGTCTGGCAAGATTCGTGGGCCTATAGTTCCAGAACGGTAATATTTTGATACTTGCATTTGTCTTAGCCTTTGGTAACTTGTTAGAGTGAATTTCTGTGTCTTGTTAATCTAGCCTTCCAAACTCTTTGTAGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGATACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTCGGATACGTTGTCCAAAGTAGGAATTCGTGCAGAGGTAGACATTAGGGAGAACTATTCTCCTAGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTTAGATATTCTGTGTATTAG

mRNA sequence

ATGAAGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTGGCGATTCCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGCATTTGTCCTAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAAGGGGAGAAAGCTATGGTCTGGCAAGATTCGTGGGCCTATAGTTCCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGATACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTCGGATACGTTGTCCAAAGTAGGAATTCGTGCAGAGGTAGACATTAGGGAGAACTATTCTCCTAGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTTAGATATTCTGTGTATTAG

Coding sequence (CDS)

ATGAAGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTGGCGATTCCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGCATTTGTCCTAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAAATCAACTTTGAAAATGAAAAAGGGGAGAAAGCTATGGTCTGGCAAGATTCGTGGGCCTATAGTTCCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCCCAAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGATACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTCGGATACGTTGTCCAAAGTAGGAATTCGTGCAGAGGTAGACATTAGGGAGAACTATTCTCCTAGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTTAGATATTCTGTGTATTAG

Protein sequence

MKSFLFQVLEILELYRRIYEEYLAIPRARRVRWRSLLVAFVLNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVIIVPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIGPKDLANNQVRYSVY

Homology

[Close]

BLAST of PI0014700 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.7e-76
Identity = 133/190 (70.00%), Postives = 161/190 (84.74%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYLA+P  + ++          + + + AF+ NTGRG+QGATSHCL
Sbjct: 218 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 277

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEK E  MVWQ+SWAYS+RTIGVM+M HGDDKGLV+PPKVAS+QV++
Sbjct: 278 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 337

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           +PVPYKDA+TQGI+DAC+AT+  L + GIRAE D+R+NYSP WKYS WEMKGVPLRIEIG
Sbjct: 338 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 397

[Close]

BLAST of PI0014700 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 189.9 bits (481), Expect = 2.7e-47
Identity = 97/194 (50.00%), Postives = 133/194 (68.56%), Query Frame = 0


Query: 7    QVLEILELYRRIYEEYLAIP--RARRVR--------WRSLLVAFVLNTGRGIQGATSHCL 66
            +VL+ILELY R+YEE LAIP  R R+          + + + AF+  +GR IQGATSH L
Sbjct: 1185 EVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHL 1244

Query: 67   GQNFAKMFEINFENEK--GEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQV 126
            GQNF+KM EI FE+ K  GEK   +Q SW  ++RTIGVMVMVHGD+ GLV+PP+VAS+QV
Sbjct: 1245 GQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQV 1304

Query: 127  IIVPVPYKDA----DTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVP 185
            +++P    +A    D + +   C+     L    IR  VD+R+NYSP WK++HWE+KGVP
Sbjct: 1305 VVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVP 1364

[Close]

BLAST of PI0014700 vs. ExPASy Swiss-Prot
Match: Q7SIA2 (Bifunctional glutamate/proline--tRNA ligase OS=Cricetulus griseus OX=10029 GN=EPRS1 PE=1 SV=3)

HSP 1 Score: 188.0 bits (476), Expect = 1.0e-46
Identity = 95/194 (48.97%), Postives = 133/194 (68.56%), Query Frame = 0


Query: 7    QVLEILELYRRIYEEYLAIP--RARRVR--------WRSLLVAFVLNTGRGIQGATSHCL 66
            +V++ILELY R+YEE LAIP  R R+          + + + AF+  +GR IQGATSH L
Sbjct: 1184 EVMQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHL 1243

Query: 67   GQNFAKMFEINFENEK--GEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQV 126
            GQNF+KM EI FE+ K  GEK   +Q SW  ++RTIGVM+MVHGD+ GLV+PP+VA +QV
Sbjct: 1244 GQNFSKMCEIVFEDPKTPGEKQFAFQCSWGLTTRTIGVMIMVHGDNMGLVLPPRVACVQV 1303

Query: 127  IIVPVPYKDA----DTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVP 185
            +++P    +A    D + +   C+     L  V IR  VD+R+NYSP WK++HWE+KGVP
Sbjct: 1304 VVIPCGITNALSEEDREALMAKCNEYRKRLLGVNIRVRVDLRDNYSPGWKFNHWELKGVP 1363

[Close]

BLAST of PI0014700 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 186.8 bits (473), Expect = 2.3e-46
Identity = 93/194 (47.94%), Postives = 132/194 (68.04%), Query Frame = 0


Query: 7    QVLEILELYRRIYEEYLAIP--RARRVR--------WRSLLVAFVLNTGRGIQGATSHCL 66
            +VL+IL+LY ++YEE LAIP  + R+          + + + AF+  +GR IQG TSH L
Sbjct: 1185 EVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHL 1244

Query: 67   GQNFAKMFEINFENEK--GEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQV 126
            GQNF+KMFEI FE+ K  GEK   +Q+SW  ++RTIGVM MVHGD+ GLV+PP+VA +QV
Sbjct: 1245 GQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQV 1304

Query: 127  IIVPVPYKDA----DTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVP 185
            +I+P    +A    D + +   C+     L  V IR   D+R+NYSP WK++HWE+KGVP
Sbjct: 1305 VIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVP 1364

[Close]

BLAST of PI0014700 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 5.0e-46
Identity = 97/194 (50.00%), Postives = 131/194 (67.53%), Query Frame = 0


Query: 7    QVLEILELYRRIYEEYLAIP--RARRVR--------WRSLLVAFVLNTGRGIQGATSHCL 66
            +VL+IL+LY  +Y   LAIP  + R+          + + + AF+  +GR IQGATSH L
Sbjct: 1386 EVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHL 1445

Query: 67   GQNFAKMFEINFEN-EKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVI 126
            GQNF+KMFEI +E+ E  +K  V+Q+SW  ++RTIGVM+MVH D++GLV+PP VA IQ I
Sbjct: 1446 GQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHADNQGLVLPPHVACIQAI 1505

Query: 127  IVP----VPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPL 186
            +VP    V  KD +   + DAC A    L   G+R E D R+NYSP WK++HWE+KGVPL
Sbjct: 1506 VVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDNYSPGWKFNHWELKGVPL 1565

[Close]

BLAST of PI0014700 vs. ExPASy TrEMBL
Match: A0A0A0KMY8 (HGTP_anticodon domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G126710 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.3e-85
Identity = 163/200 (81.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   MKSFLFQVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQG 60
           M+SFLFQVLEILELYRRIYEEYL IP  +  +          + + + AF+ NTGRGIQG
Sbjct: 1   MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG 60

Query: 61  ATSHCLGQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVA 120
           ATSHCLGQNFAK+FEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMP +VA
Sbjct: 61  ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA 120

Query: 121 SIQVIIVPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVP 180
           SIQVIIVPVPYKDADTQGIF ACSATSD LSK GIRAEVDI ENYSP WKYSHWEMKGVP
Sbjct: 121 SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 180

Query: 181 LRIEIGPKDLANNQVRYSVY 191
           LRIEIGPKDLANNQVRY VY
Sbjct: 181 LRIEIGPKDLANNQVRYFVY 200

[Close]

BLAST of PI0014700 vs. ExPASy TrEMBL
Match: A0A1S4DS87 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.2e-84
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. ExPASy TrEMBL
Match: A0A1S3CR78 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.2e-84
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 149 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 208

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 209 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 268

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 269 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 328

[Close]

BLAST of PI0014700 vs. ExPASy TrEMBL
Match: A0A1S3CR06 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.2e-84
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. ExPASy TrEMBL
Match: A0A1S3CQZ1 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.2e-84
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. NCBI nr
Match: XP_008466319.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X2 [Cucumis melo])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. NCBI nr
Match: XP_008466343.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 149 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 208

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 209 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 268

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 269 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 328

[Close]

BLAST of PI0014700 vs. NCBI nr
Match: XP_008466335.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X3 [Cucumis melo])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. NCBI nr
Match: XP_008466310.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_008466350.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_016898851.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 158/190 (83.16%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFDACSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

[Close]

BLAST of PI0014700 vs. NCBI nr
Match: KAA0050272.1 (proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 318.2 bits (814), Expect = 4.9e-83
Identity = 157/190 (82.63%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLAIP  +  +          + + + AF+ NTGRGIQGATSHCL
Sbjct: 200 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 259

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEKGEKAMVWQ+SWAYS+RTIGVMVMVHGDDKGLVMPPKVAS+QVII
Sbjct: 260 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 319

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIFD CSATSD LSK GIRAEVDIRENYSP WKYSHWEMKGVPLRIEIG
Sbjct: 320 VPVPYKDADTRGIFDVCSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 379

[Close]

BLAST of PI0014700 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 1.9e-77
Identity = 133/190 (70.00%), Postives = 161/190 (84.74%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYLA+P  + ++          + + + AF+ NTGRG+QGATSHCL
Sbjct: 218 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 277

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEK E  MVWQ+SWAYS+RTIGVM+M HGDDKGLV+PPKVAS+QV++
Sbjct: 278 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 337

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           +PVPYKDA+TQGI+DAC+AT+  L + GIRAE D+R+NYSP WKYS WEMKGVPLRIEIG
Sbjct: 338 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 397

[Close]

BLAST of PI0014700 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 1.9e-77
Identity = 133/190 (70.00%), Postives = 161/190 (84.74%), Query Frame = 0


Query: 7   QVLEILELYRRIYEEYLAIPRARRVR----------WRSLLVAFVLNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYLA+P  + ++          + + + AF+ NTGRG+QGATSHCL
Sbjct: 218 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 277

Query: 67  GQNFAKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVII 126
           GQNFAKMFEINFENEK E  MVWQ+SWAYS+RTIGVM+M HGDDKGLV+PPKVAS+QV++
Sbjct: 278 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 337

Query: 127 VPVPYKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIG 186
           +PVPYKDA+TQGI+DAC+AT+  L + GIRAE D+R+NYSP WKYS WEMKGVPLRIEIG
Sbjct: 338 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 397

[Close]

BLAST of PI0014700 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 78/185 (42.16%), Postives = 117/185 (63.24%), Query Frame = 0


Query: 10  EILELYRRIYEEYLAIP--RARRVRWRSLLVAFVLNT-------GRGIQGATSHCLGQNF 69
           +++E+Y R   E  AIP    R+ +  +   A +  T        + +Q  TSH LGQNF
Sbjct: 236 QMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDRKALQAGTSHNLGQNF 295

Query: 70  AKMFEINFENEKGEKAMVWQDSWAYSSRTIGVMVMVHGDDKGLVMPPKVASIQVIIVPVP 129
           ++ F   F +E GE+  VWQ SWA S+R +G ++M HGDD GL++PPK+A IQV+IVP+ 
Sbjct: 296 SRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPIW 355

Query: 130 YKDADTQGIFDACSATSDTLSKVGIRAEVDIRENYSPRWKYSHWEMKGVPLRIEIGPKDL 186
            KD +  G+  A S+  + L   G+R ++D  +  +P WK++ WEMKG+PLRIEIGP+D+
Sbjct: 356 KKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPRDV 415

[Close]

BLAST of PI0014700 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 122.1 bits (305), Expect = 4.8e-28
Identity = 59/93 (63.44%), Postives = 69/93 (74.19%), Query Frame = 0


Query: 95  MVHGDDKGLVMPPKVASIQVIIVPVPYKD-ADTQGIFDACSATSDTLSKVGIRAEVDIRE 154
           M HGDDKGLV PPKVA +QV+++ VP K  AD Q + DAC A   TL   GIRAE DIR+
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 155 NYSPRWKYSHWEMKGVPLRIEIGPKDLANNQVR 187
           NYS  WKY+  E+ GVPLRIE GP+DLAN+QVR
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVR 181

The following BLAST results are available for this feature:

BLAST of PI0014700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1R2	2.7e-76	70.00	Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P...	[more]
Q8CGC7	2.7e-47	50.00	Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE...	[more]
Q7SIA2	1.0e-46	48.97	Bifunctional glutamate/proline--tRNA ligase OS=Cricetulus griseus OX=10029 GN=EP...	[more]
P07814	2.3e-46	47.94	Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=...	[more]
P28668	5.0e-46	50.00	Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G...	[more]

BLAST of PI0014700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMY8	3.3e-85	81.50	HGTP_anticodon domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G126...	[more]
A0A1S4DS87	8.2e-84	83.16	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A1S3CR78	8.2e-84	83.16	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A1S3CR06	8.2e-84	83.16	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A1S3CQZ1	8.2e-84	83.16	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]

BLAST of PI0014700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466319.1	1.7e-83	83.16	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X2 [Cucumis melo]	[more]
XP_008466343.1	1.7e-83	83.16	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo]	[more]
XP_008466335.1	1.7e-83	83.16	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X3 [Cucumis melo]	[more]
XP_008466310.1	1.7e-83	83.16	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_00846...	[more]
KAA0050272.1	4.9e-83	82.63	proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase...	[more]

BLAST of PI0014700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62120.1	1.9e-77	70.00	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	1.9e-77	70.00	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G52520.1	1.5e-40	42.16	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	4.8e-28	63.44	tRNA synthetase-related / tRNA ligase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 2..106 e-value: 5.3E-27 score: 97.0
None	No IPR available	PANTHER	PTHR43382:SF4	PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKE	coord: 7..186
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 101..187
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 8..119
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 113..187 e-value: 7.6E-14 score: 51.6
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 107..189 e-value: 2.1E-27 score: 97.2
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 7..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014700.1	PI0014700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006433	prolyl-tRNA aminoacylation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity