PI0014570 (gene) Melon (PI 482460) v1

Overview


Name	PI0014570
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Ribonuclease 3-like protein 1
Location	chr07: 1437485 .. 1439942 (-)
RNA-Seq Expression	PI0014570
Synteny	PI0014570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACAGTACACAGAAATCGTCGGAGCAGAGCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCAAGATTAAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATGTAACTGGGAGTCACTCCTCGATTCCGGTAAAACTCTGTTGATTTCATGCGATTGTATTCGATTTTCTTTGGAATTTGAAACCCTTTTAATTTCGATCTGACTTGTTTCAGGCTAAATTTGAGAAATCTAGGTATATTAGACGCGTTCCTCAGTTTATGCCGTCTGATCACCGGCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTAGCGTGTTCTGATTCCGCTCGAGCTCTGAATACGAAAACCATTCCGGTGGCTCCGTCGGCGGATTCCTTCTTCAAAAGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTACCGGTTTAAACCTAGTTCCTCAACTTCATTGATTGTTATTTGGAATTCGAGATGGATGATTCTTGTTATGTGTTATCATTTTCAGTGATTCTATGATTTTCTTCTGACAATTGATTGTTCAACTGATCTCATGCATTTCATCAGGAACGCCGTCTTTATCGCCTGTTTACCCCTAATCACTTCCCAATTCGTCATTAGTATTTGTCCATTAATTTCAAAGTGCATTTAAAAAAGAAAGAAAGAAGATGTAAGCATTTGGTCTCTAAATTTCCAAAACAGAACTCTGGATCAAGATCATTTTCAGGATCATGTTTGAACAGTTTAATTTCTTTCAATTATTTCTTTCGATTCTTCCCAGGATGACTGTTTTCAATAATGAATGAAGTTGTGTGTGAATGATAACATGCATTAGACTTCATAAATCATTCGATCACAATCCTCCGTGGCAGAAAGTTTTGAGAATGTTTTTGAATTTATGTTTCTTCCTCTCATTTTTTAGTTATAATTTTTAATTAGTCAAGAAACATTCGTCAAATTCTAAAATTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCCTTGAAAACTTTTTGTTTAACTGTGACTTGTTTTATTTTAAACATTAGTAGAAAGTGAATTATAAAATAGAAACTTATGGATGGTATTAGTATTTATAAACTTAATAACTAAAAACTTATCAAATGACTTATAATTTATTACTTCTTTTTCTCCAAAGAACAAGTTATGATGCTCTGCTCTTGAGAGCTGGAATCTCAAAATATCACTTCGAATATGTAAATAGGAGACTAAATTATTTAAATCTTTGTTGTGCTTTGGAGTTGAGCTCTCTAGAGACTACTGGGTGAGTCATAAGCATAATGCTAGGAGTGAGTATCATTCTCCAATTACCAATTAATAAATCAGTTATTCAAACATGTTTATACCTTTTTTCCCTTAATTAAAAAGATGGAATACAATTTTGCAACTTAAATATCAAAAAGCACATAAGCTTTTCACTTTGATGCCTTACTTGATCTTCGTTTAAGTTGGATGTTGGATGAGGAAGACTGCTTATTGCCTCTCTGATTCCCATAAGCATTGCCATGGCGCTTCAGAAATTTTGATTTTGACTACTCAAAGTTTGCTCTAATGGTTTGGCTTCACCATTTTTCATTTTAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGCGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTAAGCTCAATATAACTCTCATCAACATAAGTTATAAACTTACAAACTCTTTGCTCAAGTAACCTATCAGGGCCAAACTTTCAACGATCTCTGTAACGTACTTAGCTAAAGATGTATTCCTTATTATGCCTTTTGGTCTTATAACGCGATAGTACTCAAAAGTTGCCCTTTTCTAACAAATACTTTTTGAAGCTTTATAGCGTTTACAGCCTTACTTGATCTAAATTTGTTTAAAAGCTGTACAACTCTGTTGCTCCTTTTTATATTCAAACGAGTACAATTAGATGTCACCATGAAAACAGCTGATCAAGGTTTCTTTTTAGCTATGTTTGGATTGACATGTCCATATAGTATCTGTACTGTGCAAACATGAGCAAGAATATAGATTGGATCGAAAGGAAAAACACACCCGAGCAAAGAATCAAATTAGTAACTAGGTCCATTTATGGAAAACAGGGTAATTTGATGGGATTTTAGCAATTGATGCAAAAATTTGATTTTGATTCAGGTATAGATTCAAGGTTAGTATTGAGATGAAGGGAGATTGTGAAGCAGGTTTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCCGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGGATATCGTTTTAAAAGATAA

mRNA sequence

ATGACACAGTACACAGAAATCGTCGGAGCAGAGCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCAAGATTAAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATGTAACTGGGAGTCACTCCTCGATTCCGGCTAAATTTGAGAAATCTAGGTATATTAGACGCGTTCCTCAGTTTATGCCGTCTGATCACCGGCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTAGCGTGTTCTGATTCCGCTCGAGCTCTGAATACGAAAACCATTCCGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGCGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTATAGATTCAAGGTTAGTATTGAGATGAAGGGAGATTGTGAAGCAGGTTTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCCGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGGATATCGTTTTAAAAGATAA

Coding sequence (CDS)

ATGACACAGTACACAGAAATCGTCGGAGCAGAGCGGAAGAATCGAGGAATTCAGATGGAGAACAATCCTTCTTGTCCTCCAAGATTAAATATCAATCTCAAAAATCTTCCTCCAATTAATAACCCGCGCAATGTAACTGGGAGTCACTCCTCGATTCCGGCTAAATTTGAGAAATCTAGGTATATTAGACGCGTTCCTCAGTTTATGCCGTCTGATCACCGGCCGGAAATAAGGTCGAATGATTACGCTGGCTTGCCGATGAAACTAGCGTGTTCTGATTCCGCTCGAGCTCTGAATACGAAAACCATTCCGAAAGATAATTGCAAGGATTTAGCTTCGAGGTGTAGTTGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGAAAAGAGAGCGGCAAAGTCCCTTCTATTTGAGATATGCACTGCAAATCACTGGCAGCCTCCTCTATTTGAATGCTGCGAAGAAGAAGGTCCAAGCCATGCAAAAAAGTATAGATTCAAGGTTAGTATTGAGATGAAGGGAGATTGTGAAGCAGGTTTGGAATGCTATGGAAACCTTCAAACAAGAAAGAAAGTGGCCGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGGATATCGTTTTAAAAGATAA

Protein sequence

MTQYTEIVGAERKNRGIQMENNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIPKDNCKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR

Homology

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BLAST of PI0014570 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 6.8e-23
Identity = 48/88 (54.55%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 124  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLEC 183
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 184  YGNLQTRKKVAAEHAAEGALWYLKHLGY 212
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of PI0014570 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.8e-21
Identity = 43/81 (53.09%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 131  RAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLECYGNLQTR 190
            + A+S LFE+C AN+W+PP F+ C+EEGPSH +K+ +KV +E+KG     LEC+ + + +
Sbjct: 1568 KTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGASATLLECHSDGKLQ 1627

Query: 191  KKVAAEHAAEGALWYLKHLGY 212
            KK A EHAA+GALW LK LG+
Sbjct: 1628 KKAAQEHAAQGALWCLKQLGH 1648

[Close]

BLAST of PI0014570 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.5e-19
Identity = 39/86 (45.35%), Postives = 61/86 (70.93%), Query Frame = 0


Query: 125 EGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDC-EAGLEC 184
           E    + +AKS+L E+C +  W+PP++ECC  +GP H + + +KV +E++    +  LEC
Sbjct: 107 ESKTRRGSAKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLEC 166

Query: 185 YGNLQTRKKVAAEHAAEGALWYLKHL 210
           +G+ + +KK AAEHAAEGALWYL+H+
Sbjct: 167 FGDPRRKKKAAAEHAAEGALWYLEHV 192

[Close]

BLAST of PI0014570 vs. ExPASy Swiss-Prot
Match: Q9FKF0 (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.4e-04
Identity = 23/63 (36.51%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 137 LFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLECYGNLQTRKKVAAE 196
           L EICT N W  P+F   EE+GP +  K+   V IE+  + E      G+++++KK A  
Sbjct: 312 LIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIP-NIEGTFHMKGDIKSKKKQAEN 371

Query: 197 HAA 200
             A
Sbjct: 372 SLA 373

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BLAST of PI0014570 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.1e-112
Identity = 201/228 (88.16%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQME-NNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  YTEIVGAERKNRGIQME NNPSCPPRLNINLKNLPPINNPRNVTG+HSSIPAKFEKS
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLP-MKLACSDSARALNTKTIP-----------KDN 120
           RYIRRVPQF+P DHRPEIRSN+Y GLP MKLACSDSARA+NTKTIP           KDN
Sbjct: 61  RYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDN 120

Query: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRF 180
           CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRF
Sbjct: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRF 180

Query: 181 KVSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           KVSIEMKGDCEA +ECYGNLQTRKK+AAEHAAEGALWYLK+LGYRF+R
Sbjct: 181 KVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of PI0014570 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.1e-112
Identity = 201/227 (88.55%), Postives = 205/227 (90.31%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQMENNPSC-PPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  Y++IVG ERKNRGIQMENNPSC PPRLNINLKNLPPINNPRNV+GSHSSIPAKFEKS
Sbjct: 1   MRHYSDIVGPERKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIP-----------KDNC 120
           RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLA SDSARALNTKT P           KD C
Sbjct: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTC 120

Query: 121 KDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180
           KDLASRCSCCRH STKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK
Sbjct: 121 KDLASRCSCCRHDSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180

Query: 181 VSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           V IEMKGDCEA +ECYGNLQTRKKVAAEHAAEGALWYL HLGYRFKR
Sbjct: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of PI0014570 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.1e-112
Identity = 201/228 (88.16%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQME-NNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  YTEIVGAERKNRGIQME NNPSCPPRLNINLKNLPPINNPRNVTG+HSSIPAKFEKS
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLP-MKLACSDSARALNTKTIP-----------KDN 120
           RYIRRVPQF+P DHRPEIRSN+Y GLP MKLACSDSARA+NTKTIP           KDN
Sbjct: 61  RYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDN 120

Query: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRF 180
           CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRF
Sbjct: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRF 180

Query: 181 KVSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           KVSIEMKGDCEA +ECYGNLQTRKK+AAEHAAEGALWYLK+LGYRF+R
Sbjct: 181 KVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of PI0014570 vs. ExPASy TrEMBL
Match: A0A1S4DZP1 (uncharacterized protein LOC103494682 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.8e-82
Identity = 155/177 (87.57%), Postives = 160/177 (90.40%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQME-NNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  YTEIVGAERKNRGIQME NNPSCPPRLNINLKNLPPINNPRNVTG+HSSIPAKFEKS
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLP-MKLACSDSARALNTKTIP-----------KDN 120
           RYIRRVPQF+P DHRPEIRSN+Y GLP MKLACSDSARA+NTKTIP           KDN
Sbjct: 61  RYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDN 120

Query: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKK 165
           CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+K
Sbjct: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARK 177

[Close]

BLAST of PI0014570 vs. ExPASy TrEMBL
Match: A0A6J1GW48 (ribonuclease 3-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458030 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 7.4e-81
Identity = 150/211 (71.09%), Postives = 168/211 (79.62%), Query Frame = 0


Query: 4   YTEIVGAERKNRGIQMENNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKSRYIR 63
           Y EIVG ERKNR  QMEN PSCP  +N+NLKNLPPI+NP     +HSSIPAKFEKSRYI 
Sbjct: 7   YREIVGEERKNRENQMENTPSCPRSVNVNLKNLPPIHNPCVGNANHSSIPAKFEKSRYIG 66

Query: 64  RVPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIP---KDNCKDLASRCSCCRH 123
           RVPQFMP +H P++R N+Y G+PM  AC DSAR+ N +T P   K+N  D ASR S C H
Sbjct: 67  RVPQFMPPNHSPDVRPNEYDGVPMNPACFDSARSPNARTTPKERKENFGDSASRSSGCSH 126

Query: 124 GSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAG 183
            STKEGT  K++AKSLL+EICTANHW+PPLFECCEEEGPSHAKKY FKV +EMKGDC   
Sbjct: 127 DSTKEGTSVKKSAKSLLYEICTANHWKPPLFECCEEEGPSHAKKYTFKVIVEMKGDCGED 186

Query: 184 LECYGNLQTRKKVAAEHAAEGALWYLKHLGY 212
           LECYG  QTRKKVAAE AAEGA+WYLKHLGY
Sbjct: 187 LECYGKPQTRKKVAAEDAAEGAVWYLKHLGY 217

[Close]

BLAST of PI0014570 vs. NCBI nr
Match: XP_008454206.1 (PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA0044459.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa] >TYK29586.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 201/228 (88.16%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQME-NNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  YTEIVGAERKNRGIQME NNPSCPPRLNINLKNLPPINNPRNVTG+HSSIPAKFEKS
Sbjct: 1   MRHYTEIVGAERKNRGIQMEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLP-MKLACSDSARALNTKTIP-----------KDN 120
           RYIRRVPQF+P DHRPEIRSN+Y GLP MKLACSDSARA+NTKTIP           KDN
Sbjct: 61  RYIRRVPQFIPFDHRPEIRSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDN 120

Query: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRF 180
           CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRF
Sbjct: 121 CKDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRF 180

Query: 181 KVSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           KVSIEMKGDCEA +ECYGNLQTRKK+AAEHAAEGALWYLK+LGYRF+R
Sbjct: 181 KVSIEMKGDCEAVVECYGNLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of PI0014570 vs. NCBI nr
Match: XP_004152191.1 (uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical protein Csa_014441 [Cucumis sativus])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 201/227 (88.55%), Postives = 205/227 (90.31%), Query Frame = 0


Query: 1   MTQYTEIVGAERKNRGIQMENNPSC-PPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKS 60
           M  Y++IVG ERKNRGIQMENNPSC PPRLNINLKNLPPINNPRNV+GSHSSIPAKFEKS
Sbjct: 1   MRHYSDIVGPERKNRGIQMENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKS 60

Query: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIP-----------KDNC 120
           RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLA SDSARALNTKT P           KD C
Sbjct: 61  RYIRRVPQFMPSDHRPEIRSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTC 120

Query: 121 KDLASRCSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180
           KDLASRCSCCRH STKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK
Sbjct: 121 KDLASRCSCCRHDSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFK 180

Query: 181 VSIEMKGDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           V IEMKGDCEA +ECYGNLQTRKKVAAEHAAEGALWYL HLGYRFKR
Sbjct: 181 VRIEMKGDCEAVVECYGNLQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of PI0014570 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 345.5 bits (885), Expect = 3.3e-91
Identity = 173/221 (78.28%), Postives = 185/221 (83.71%), Query Frame = 0


Query: 5   TEIVGAERKNRGIQMENNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKSRYIRR 64
           TEIV AERKNRG QMEN  SCP RLN NLKNLPPINN    TG+HSSI AKFEKSRYIRR
Sbjct: 4   TEIVEAERKNRGNQMENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRR 63

Query: 65  VPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIP----------KDNCKDLASR 124
           VPQF+P +HRP    N+YAGLPMK ACS SARALN+KTIP          KDN +++ASR
Sbjct: 64  VPQFIPQNHRP----NEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASR 123

Query: 125 CSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMK 184
            SCC HGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKV++EMK
Sbjct: 124 SSCCGHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMK 183

Query: 185 GDCEAGLECYGNLQTRKKVAAEHAAEGALWYLKHLGYRFKR 216
           G  E  LECYGNLQTRKKVAAEHAAEGA+WYLK+LGY FKR
Sbjct: 184 GASETVLECYGNLQTRKKVAAEHAAEGAIWYLKNLGYCFKR 220

[Close]

BLAST of PI0014570 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 328.2 bits (840), Expect = 5.4e-86
Identity = 164/210 (78.10%), Postives = 175/210 (83.33%), Query Frame = 0


Query: 5   TEIVGAERKNRGIQMENNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKSRYIRR 64
           TEIV AERKNRG QMEN  SCP RLN NLKNLPPINN    TG+HSSI AKFEKSRYIRR
Sbjct: 4   TEIVEAERKNRGNQMENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRR 63

Query: 65  VPQFMPSDHRPEIRSNDYAGLPMKLACSDSARALNTKTIP----------KDNCKDLASR 124
           VPQF+P +HRP    N+YAGLPMK ACS SARALN+KTIP          KDN +++ASR
Sbjct: 64  VPQFIPQNHRP----NEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASR 123

Query: 125 CSCCRHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMK 184
            SCC HGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKV++EMK
Sbjct: 124 SSCCGHGSTKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMK 183

Query: 185 GDCEAGLECYGNLQTRKKVAAEHAAEGALW 205
           G  E  LECYGNLQTRKKVAAEHAAEGA+W
Sbjct: 184 GASETVLECYGNLQTRKKVAAEHAAEGAIW 209

[Close]

BLAST of PI0014570 vs. NCBI nr
Match: XP_038878793.1 (ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 324.3 bits (830), Expect = 7.8e-85
Identity = 161/207 (77.78%), Postives = 173/207 (83.57%), Query Frame = 0


Query: 19  MENNPSCPPRLNINLKNLPPINNPRNVTGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEIR 78
           MEN  SCP RLN NLKNLPPINN    TG+HSSI AKFEKSRYIRRVPQF+P +HRP   
Sbjct: 1   MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRP--- 60

Query: 79  SNDYAGLPMKLACSDSARALNTKTIP----------KDNCKDLASRCSCCRHGSTKEGTP 138
            N+YAGLPMK ACS SARALN+KTIP          KDN +++ASR SCC HGSTKEGTP
Sbjct: 61  -NEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTP 120

Query: 139 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLECYGNLQ 198
           EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKV++EMKG  E  LECYGNLQ
Sbjct: 121 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQ 180

Query: 199 TRKKVAAEHAAEGALWYLKHLGYRFKR 216
           TRKKVAAEHAAEGA+WYLK+LGY FKR
Sbjct: 181 TRKKVAAEHAAEGAIWYLKNLGYCFKR 203

[Close]

BLAST of PI0014570 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 109.0 bits (271), Expect = 4.8e-24
Identity = 48/88 (54.55%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 124  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLEC 183
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 184  YGNLQTRKKVAAEHAAEGALWYLKHLGY 212
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of PI0014570 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 109.0 bits (271), Expect = 4.8e-24
Identity = 48/88 (54.55%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 124  KEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCEAGLEC 183
            K G P  + AKSLL E C AN W+PP FECCEEEGP H K + +KV +E++      LEC
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1658

Query: 184  YGNLQTRKKVAAEHAAEGALWYLKHLGY 212
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1659 YGEARATKKGAAEHAAQAAIWCLKHSGF 1686

[Close]

BLAST of PI0014570 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-20
Identity = 39/86 (45.35%), Postives = 61/86 (70.93%), Query Frame = 0


Query: 125 EGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDC-EAGLEC 184
           E    + +AKS+L E+C +  W+PP++ECC  +GP H + + +KV +E++    +  LEC
Sbjct: 107 ESKTRRGSAKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLEC 166

Query: 185 YGNLQTRKKVAAEHAAEGALWYLKHL 210
           +G+ + +KK AAEHAAEGALWYL+H+
Sbjct: 167 FGDPRRKKKAAAEHAAEGALWYLEHV 192

[Close]

BLAST of PI0014570 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-19
Identity = 45/90 (50.00%), Postives = 61/90 (67.78%), Query Frame = 0


Query: 123 TKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCE-AGL 182
           T E   ++ +AKS L+ +C+  HW+ PL+E    EGP H K +  KV++EMK D     L
Sbjct: 75  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 134

Query: 183 ECYGNLQTRKKVAAEHAAEGALWYLKHLGY 212
           EC+GN Q +KK+AAE AAE ALWYLK++GY
Sbjct: 135 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 163

[Close]

BLAST of PI0014570 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-19
Identity = 45/90 (50.00%), Postives = 61/90 (67.78%), Query Frame = 0


Query: 123 TKEGTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVSIEMKGDCE-AGL 182
           T E   ++ +AKS L+ +C+  HW+ PL+E    EGP H K +  KV++EMK D     L
Sbjct: 53  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 112

Query: 183 ECYGNLQTRKKVAAEHAAEGALWYLKHLGY 212
           EC+GN Q +KK+AAE AAE ALWYLK++GY
Sbjct: 113 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 141

The following BLAST results are available for this feature:

BLAST of PI0014570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P84634	6.8e-23	54.55	Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2	[more]
A7LFZ6	2.8e-21	53.09	Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
Q9M8N2	3.5e-19	45.35	Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1	[more]
Q9FKF0	5.4e-04	36.51	Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1	[more]

BLAST of PI0014570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E0Z9	8.1e-112	88.16	Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A0A0KYI7	8.1e-112	88.55	DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S...	[more]
A0A1S3BXK0	8.1e-112	88.16	uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S4DZP1	1.8e-82	87.57	uncharacterized protein LOC103494682 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1GW48	7.4e-81	71.09	ribonuclease 3-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of PI0014570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008454206.1	1.7e-111	88.16	PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA00...	[more]
XP_004152191.1	1.7e-111	88.55	uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical ...	[more]
XP_038878769.1	3.3e-91	78.28	ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib...	[more]
XP_038878786.1	5.4e-86	78.10	ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida]	[more]
XP_038878793.1	7.8e-85	77.78	ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida]	[more]

BLAST of PI0014570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20320.1	4.8e-24	54.55	dicer-like 4	[more]
AT5G20320.2	4.8e-24	54.55	dicer-like 4	[more]
AT1G80650.1	2.5e-20	45.35	RNAse THREE-like protein 1	[more]
AT4G00420.2	2.1e-19	50.00	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]
AT4G00420.3	2.1e-19	50.00	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 133..207 e-value: 9.7E-7 score: 38.4
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 132..208 score: 9.416368
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 133..207 e-value: 6.3E-14 score: 52.0
None	No IPR available	GENE3D	3.30.160.20		coord: 139..208 e-value: 1.2E-20 score: 75.7
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 11..215
None	No IPR available	PANTHER	PTHR14950:SF67	DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEIN	coord: 11..215
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 133..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014570.1	PI0014570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding