PI0014446 (gene) Melon (PI 482460) v1

Overview


Name	PI0014446
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	gibberellin 2-beta-dioxygenase 8
Location	chr12: 4139503 .. 4145355 (+)
RNA-Seq Expression	PI0014446
Synteny	PI0014446

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCACACTCTCTCCAATTATATTTCCCAACCCTTTTTCCCTTTTCAATTACTATATTAACTTTCATCTCTTTGAAAGTGACATCCCCCTTTTTAATTATTTTTCCCATTTTCCAATATCTCTCCAAGGTAAATATAATAAATAATCACCATAACATTAATATCTCTAGTGCTCTACTCTCATCTACTCGTCCAATTAACTTATATTCCATATTAATCACTCCATTCATTTTTGCAGACCATTTCAAAGAAGCAAAAATGACACAGACTCTAAATCAAGTAAGTTTCGTTTCATTTTTATTTTATTAGTTGAACTATATTCGAGTTGACTTTCTTATGTGTTAAGAAAATGACGATGGTTTTATATATTTATATAAATAGATTTAGTTTTTCTTTTTCTATATTTTTTCCAACTACTTTTGTTTATTCGTTTTGTTAAGAGCTGTTGTGTCTGTACACAAGAATGGGCACACATCGAACCATATGTTTGGCCATTTTAGATCTTTCCCGATTTTTATAATATATTTTTATATAAATGGCTTCATAATTTAAACTGTAATTGTCGCTCTTTGTTTTTTCAGAAAAGTTCCAAGTATCTAAATAATGCTCTTTTTATATTTTTATATTATATTTTATTTATAAAAATTAAAAAAAAAAAAAAAAAACACCAAAATTAAATTCTCTCTATCTTTTTTTGTTGTTGTAATAATTGACTTTGTTTAACCATTGGATTTAAAATCGGTAATTTTTATAATTACGTTAATAAATAAAGCCATTGGTTCCTAAGATGGACACGAAGCTGGCTGTCATCTTTATCTTTATTTGCTGTATTTTTCCATTTTACAGCCACGTCCCATCAAACGAGTGTTGTACGGTTGTATAGTATTACTAAAATTATGCTTCATTTATTTAATTTAATCTTTTAATTTTAAAAACATTTCTGAATTTGAAGAATTAATCACGTCTAACATTAATATGATTGCTAAATAATAATAATAATAATAAATGGTAATATTAGGTATCAAATACACACGTAAATTAGCATCTGCATTTATGAATTTAACCTTAAATATTAATATTATTAATAAAAAGATATAAGATAAGAGTTAAATGAAAATGAATTATGATTGAAGTAGAATTGGATAAAAACAGACATCCCCTCTTCAAAAGGGGAAAAGAAGTCATATACGTGTTGAAACGCGTCAATATTAAGATTGTATAAATAAACAAATATTTTGAATACCATATTCTGTCGATAAAAGCCATTGCCCTACGCCTAGTCATTTCAACAAATAAATTTTAATAAAATCTTGATTTTCTTTTTCTTTTTCTTTTTCTTTTGGAAGAACAAATAAAATATTCGATTATTATTATTATTATTATTGTTGTTGTTGTTATTAATAATGATAATTATTATTATTGCCATTGTGTTGTAAATCAACGTTGTGGCCACAAAATAACTAGTTCATCATTTAGTGGGTCTCTCCTTTTTGAAAGGTATATGTGTTTGAAGTTTGAAGTTATATTTATGTATGTATATATACGCATTTGAATAATAAATTTTCAAATTCTATTAAGGATAGATATTGATAGACTTTTATCAGTATCTATCGTTATTAGACGAGAATTTATTATTTTGTTATATATATTGAAAATTTTTTCAAAATTTTTTTCATTTATAATATCATAAAAAAACGAAAAAGAATATACTGTCCTTCGACAAATAGAAAATATAAAAGATATAAAGAATGACCTAAGTAGTTAGATATCCTATTTTTCCCTTTCAAACCATCGTTTGTTGTATGTCTATTGAAAAATTGTTGATAATATTTACAAATATAGCAAAATATCATTGTCTATCTACGATATACTACTCATAGCATAGATAGACATACATAGTAGCCTATCACAAATAGACGGTGATATTTTGCTATTTTTGAAAAGAATTCCATAAATCTTTTATTTTCTAATTCTTTTTTACTAAATTTAAAAATCTCTTAATTGCATTATGAAAGCTACTTTTTTTTTTTTTTTTTTTTTGGTTGTTCTTAAATTTTCCTCTTTAATTATTACGTTAAAATCTGTAGTTTTCTTGACCATTTGACGTTTAAGTTTGGATTTATGATGTAATATTTTGTTAATAAATAATTTTTTAAAATTTGAAAAGAAAAATAAAGAGAATAAATTATTGATCTGTAAAATATATTTGTTAGATGCCATTACATTATAGATATTAAAAAAGAAATTGTTTTGATTGATGTATACCAACATAGAATGAATTGGAAATGGTCAAAAGATTGGGAAGAAACACATAATAGAGATAAAATAGAATTCAAATTTAGAAATGAAAATGAAGAGCATAATTATATTAGTTGAATTAAGCAGTAGAGATTTAGAATATAAATTTGATATATATTGAAGATGCAAACAAAAATTAGACATAAAAACTGTCACACCCTTTGTCTGAAAGAATAGAATAGAATAGATCTATACACTTTTTGAGGTATATGAATTGGCAAATGGCAAATATATATATTATATACATATATATTGAATGAGGCAAAGGCACATGTAATATTCAGAGATTGCCTCTTTATTACAATCGCAAACTGACATCCACTGTACTTCTTCTCGTTCCTTCCAGCACCTGATCCCTTTCTAATTAACAAACGACATTCAAATATTTGACTTTCTTTCCTTCTTTTTTTTTTTTTCTCTCACAAAAATCATATCATACAATTTTTAAATCCCTTTTTAGGTTTATTTTACTCCTTTCAAATATTTCTTTTTATTCAATTTGGATTAATTCTTTTGCACCTATATTTTAAGTTTGTCGAAGATTTCATTGATGTTTCAAACTCTTTTTTCACTCCTTATTTCGATTTAGAATATATAATCGTCTTTCAAAATTTCTGTACGTGCTGTTTTTTTAAATACATAATTTTAAAGTTACTTTCTAAAGAATTTACTTGGGTTCAAAAAAATGAGATAAATTAAGAAAAAGAATAATGGAATATAGTATTGTAAACTTTTTTTTTATACAATATACAATATTATAAACTTTTTGAAAGTAAAAGAGGGAAAAAAATTGACCAAAATAACTCTTTTGAACTTATCAAACAGTGTTCATGACAAACATATATGGAGTTTATAAAATATAAAATTAATGTCATTTAGTGTTTGTTTGGATTTTTTTTATTCATTTTCATTCGAATTAAAAAGATTTTAAAACCAATTTTCATTATGTAATTTAAAAGGTATTACTTTAATTTTTTTTCCTAAATAACTTGTTTTTAAAATTAAACCAAACACAAGTTTGACAACAGGGTGGATGAAACAAAAATCTCACTTTTTCACTACTTGAGCCCAACATCCTTAAAAAGGGAAAGGCCATATTTGAACACAAAATATTCTTTTTCCTTACAAAGATATTGCATTTTATTTTGTGTTTTTCCTTAAATTTCAGAAGAAAAGAAAGACGGTGAAAATATTTCTGCCTAAGCCTAAGCCTACAAAAACACACTATATAAATCTAAAGAACATAAACCAAAGACCAACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACATAAAGAAAAGGAAAAAAAAAGGTCTTTATTTTTTATTTTTTGCCCTTTTAATTTGTCTTCTATTTCTCCGATTAATTTGTCTTGTTATACAATAAATTTGATAATCACCACTTTTAATTTTGTCATAATTTATATCAGTCGTCTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGACTCTCTTGGCCGAAACGGCGGCGGCAAAGGGAAAGAGCGACGTCGTGACGGTGGAGGAATGGGATCTTCCGGTTGTTGATTTAGGGCGTTTGACGGCGGGGGAAGTGGAAGCGGAGGAAGAATGCAAAAATGACATAATTAGAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCCAAACAAATCGAGCTTTTTAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGATCCAACGACGGCTCCGATGATGATCTCAGGTACAAATTACACCTTTTTTTCTTCCTTTCCTTTTTTTTTTTTTTTAATGAAAAATTGTTTGAATTTTAATGGAATAAGAAACTCACCTCAACTTAAACGGTTTCGGTTTGGATTCACTTTGTTTCCGTTTTGATATAATTATTATTTTGTTTTTTGTTATTATACTAATAAACACAATTTTTATTCATAGTTTTCTTCTTTTGTCACCGTCTATTTTCAAACTTTTTTGAAACTCATACAAAAATTTGAGTAACTGAAAAACATTGGTTCTTAGAAAGTTGAAATTTTGTTTTTGAAAATTTGAAGGTCAAAATATTGAGATGATGAAGAAACATCACCATTTTTTTAAGATCCATATATCCTTTCATCACTAATTATCATTGAGACACTCTTGTTTTATTTAAACTATATTATAGTCTACAGGATCCAAACAAATTATCATATCGAACATCGGTTGAAGATCACCTATTAGAAACTTCACAATTTTGATAAGACATACGAGTTATATATCTCATTTTACTCCACACAATTACTTTTTTTAATTGAAAAACTTTGTAAATTTTAATATTATTAAAATATTTCTTTCAATCTTTAAAACAACTTTTCTAATATTTAGAAAGTCAGTCTATATCAACTTTTCTTTTTCTGTAACAAATGCACGAATATAGGTAGAGAAATATGTATACATGCATCTAAAATTATTAAAAAGTTATTAATTTGAACGTAGAGAATAAATATCCAACATACATTAAATAGTTAGGAAAACAATAATATTTAGTTTCAACGGTGGTCATTCTTCTTTTCCGCATCAATAGATGGATTAAGGAAGGATATATTTAAGGGAGATAAAAAAGAATAATATGTGTACTTTGATATTCTTGATCATAGAAGGTAGAATAGATTAAATTCTTTGATACCATACATAGGATAGAGGCAAAAAAGATATAAGAAATGAAAATGCATTCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAATTTGGGGAGAAGCTCAAAATTCTTCGTAGAAAATTGCGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTCCGGGTCAAATATTCGGTCTAATGCCACACACCGACAGCGATTTTCTCACGATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAATATAGGAGACTTATTTCAGGTAATAATTTATTATTTATATTCTTTTGTGTTTTTATCTTAATATTAAATTAAGTGAAATTAAATAAGAATAATATAATAAAATTAACATAATTGATTTTGTGCAGGCTTGGAGTAACGATGTATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCGATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTAATAAAAAGCAAGAGTGAACCAGGGGTCTACAGAAGGTTTAGTTTTAGGGAATTTAGGAATCAAGTTCAAGAAGATGTTCGTAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATCATCAAACTTCTAAACCCAATTTTTCAAAAAT

mRNA sequence

GTCACACTCTCTCCAATTATATTTCCCAACCCTTTTTCCCTTTTCAATTACTATATTAACTTTCATCTCTTTGAAAGTGACATCCCCCTTTTTAATTATTTTTCCCATTTTCCAATATCTCTCCAAGACCATTTCAAAGAAGCAAAAATGACACAGACTCTAAATCAATCGTCTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGACTCTCTTGGCCGAAACGGCGGCGGCAAAGGGAAAGAGCGACGTCGTGACGGTGGAGGAATGGGATCTTCCGGTTGTTGATTTAGGGCGTTTGACGGCGGGGGAAGTGGAAGCGGAGGAAGAATGCAAAAATGACATAATTAGAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCCAAACAAATCGAGCTTTTTAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGATCCAACGACGGCTCCGATGATGATCTCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAATTTGGGGAGAAGCTCAAAATTCTTCGTAGAAAATTGCGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTCCGGGTCAAATATTCGGTCTAATGCCACACACCGACAGCGATTTTCTCACGATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAATATAGGAGACTTATTTCAGGCTTGGAGTAACGATGTATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCGATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTAATAAAAAGCAAGAGTGAACCAGGGGTCTACAGAAGGTTTAGTTTTAGGGAATTTAGGAATCAAGTTCAAGAAGATGTTCGTAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATCATCAAACTTCTAAACCCAATTTTTCAAAAAT

Coding sequence (CDS)

ATGACACAGACTCTAAATCAATCGTCTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGACTCTCTTGGCCGAAACGGCGGCGGCAAAGGGAAAGAGCGACGTCGTGACGGTGGAGGAATGGGATCTTCCGGTTGTTGATTTAGGGCGTTTGACGGCGGGGGAAGTGGAAGCGGAGGAAGAATGCAAAAATGACATAATTAGAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCCAAACAAATCGAGCTTTTTAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGATCCAACGACGGCTCCGATGATGATCTCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAATTTGGGGAGAAGCTCAAAATTCTTCGTAGAAAATTGCGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTCCGGGTCAAATATTCGGTCTAATGCCACACACCGACAGCGATTTTCTCACGATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAATATAGGAGACTTATTTCAGGCTTGGAGTAACGATGTATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCGATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTAATAAAAAGCAAGAGTGAACCAGGGGTCTACAGAAGGTTTAGTTTTAGGGAATTTAGGAATCAAGTTCAAGAAGATGTTCGTAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATCATCAAACTTCTAA

Protein sequence

MTQTLNQSSANVAMDPPFHEAYKTLLAETAAAKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVLSSNF

Homology

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BLAST of PI0014446 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 430.6 bits (1106), Expect = 1.6e-119
Identity = 210/339 (61.95%), Postives = 255/339 (75.22%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAAAKGK---------SDVVTVEEWDLPVVDLGRLTAGEVEAEEE 73
           MDPPF+E Y  LL      K           S    VEE +LPV+D+ RL  G  E  E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 74  CKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYR 133
           CK  I RAS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 134 WGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGE 193
           WGTPSATSI QLSWSEAFHV ++DI  + D +   L STME+FA +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDISDNKDFT--TLSSTMEKFASESEALAYMLAEVLAE 180

Query: 194 NLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELV 253
             G++S FF ENCV +TCYLRMNRYPPCP P +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 254 KDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETV 313
           KD +WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN K ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 314 IKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFV 344
           I+  S+   YR FSFREFR QVQEDV+K G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of PI0014446 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.4e-91
Identity = 176/357 (49.30%), Postives = 232/357 (64.99%), Query Frame = 0


Query: 10  ANVAMDPPFHEAYKTL----------LAETAAAKGKSDVVTVEEWDLPVVDLGRLTAGEV 69
           A++A+DPP  ++Y+ L          +A  A     S    +E  DLP+VDL RLT G  
Sbjct: 5   ADIAIDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVLER-DLPMVDLERLTRGGA 64

Query: 70  EAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFS 129
              + C   + RA+ EWGFFQ+ NHG+  +L+ +MR +Q  LF+ PFE K K    LN  
Sbjct: 65  GERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAG-LLN-- 124

Query: 130 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDD--DLRSTMEEFAGKVSRLAQK 189
            GSYRWG P+ATS+  LSWSEAFHV L+ I G++    D   LR  M+E A  +SR+A  
Sbjct: 125 -GSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANT 184

Query: 190 LAEILGENL-GR------SSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFL 249
           +A  L E L GR      ++ +F   C  +TC+LR+NRYP CP     FGL+PHTDSDFL
Sbjct: 185 VAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFL 244

Query: 250 TILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKER 309
           T+L QDQVGGL L+KD +W+AV+P P AL++NIGDLFQAWSN+ YKSVEH+VV N+K +R
Sbjct: 245 TVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDR 304

Query: 310 YSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVLSSN 348
            S+AYFLCP  ++++ +  EP  YR F+F E+R +VQEDVR  G KIGLP F   S+
Sbjct: 305 LSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNFFKHSS 356

[Close]

BLAST of PI0014446 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.2e-83
Identity = 162/336 (48.21%), Postives = 215/336 (63.99%), Query Frame = 0


Query: 15  DPPFHEAYKTLLAETAAAKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASK 74
           +PP    YK L  E          + V+E +LPV+DL    AG +EAE+ C+ D++RA+ 
Sbjct: 11  NPPLMSTYKHLFVEQHRLDMDMGAIDVDECELPVIDL----AGLMEAEQVCRADMVRAAS 70

Query: 75  EWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGTPSATSIT 134
           EWGFFQV NHG+   LL ++   Q+ +F++PF+ K  E + L FS  SYRWGTP+A  + 
Sbjct: 71  EWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTE-RLLGFSPESYRWGTPTAKCLE 130

Query: 135 QLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENL-GRSSKFF 194
           QLSWSEA+H+ ++        +    R+ +EE +  +  LAQKLAEIL   L G      
Sbjct: 131 QLSWSEAYHIPMTT---PRPSTSIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGET 190

Query: 195 VENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILH--QDQVGGLELVKDGKWIA 254
           +      TC+LR+NRYPPC +    FGL PHTDSD LTI+H  QD VGGL+L+K G+W+A
Sbjct: 191 MVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVA 250

Query: 255 VKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKS-- 314
           VKP+P  LI+N+GDL QAWSNDVYKSVEHRV+ N+  ER+S+A+FLCP   T+I   S  
Sbjct: 251 VKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSH 310

Query: 315 ---EPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 343
              +   YR F+F E+R Q+ EDVR  G KIGL RF
Sbjct: 311 VHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

[Close]

BLAST of PI0014446 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 6.3e-79
Identity = 149/338 (44.08%), Postives = 218/338 (64.50%), Query Frame = 0


Query: 12  VAMDPPFHEAYKTLLA-----ETAAAKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECK 71
           +A  PPF   + ++        T+ +   +  +      LPV+DL  LT+GE    + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 72  NDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWG 131
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +F + S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 132 TPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENL 191
            PSATS  Q S SEAFH+ LS++   +D   ++LR+ +E +  +++R+AQ + EILG+ +
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEVSRISD-DRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 192 GRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKD 251
             SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  +
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 252 GKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIK 311
           G+WI+VKP  +AL +NIGD+FQA SN VY+SV HRV++ +  ER SIA+F+CP+ ET I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 312 SKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
               P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of PI0014446 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 99/289 (34.26%), Postives = 143/289 (49.48%), Query Frame = 0


Query: 41  VEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIE 100
           V+   +P++DL    +G+     E    + +A+ + GFF + NHG+   LL++       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 101 LFKQPFERKSKEDQFLNFSAGSYRWGTPS--ATSI-----TQLSWSEAFHVSLS------ 160
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 161 -----DILGSNDGSD-DDLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKFFVENCVPST 220
                D +    G   +D     +E+A  ++ L+ K+ E+LG +LG   ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 221 CYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALII 280
              R+N YP C  P    G  PH D   LTILHQDQVGGL++  D KW ++ PNP A ++
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 281 NIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 311
           NIGD F A +N  YKS  HR V NS++ER + A+FLCP  E V+K   E
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEE 332

[Close]

BLAST of PI0014446 vs. ExPASy TrEMBL
Match: A0A1S3B309 (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 6.6e-188
Identity = 328/345 (95.07%), Postives = 333/345 (96.52%), Query Frame = 0


Query: 1   MTQTLNQSSANVAMDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEV 60
           MT  L QSSANVAMDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+V
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  EAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFS 120
           E EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLA 180
           AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCP 300
           GLELVKDGKWIAVKPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

[Close]

BLAST of PI0014446 vs. ExPASy TrEMBL
Match: A0A1S3B3F3 (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 6.1e-186
Identity = 324/339 (95.58%), Postives = 330/339 (97.35%), Query Frame = 0


Query: 7   QSSANVAMDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEEC 66
           +SSANVAMDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+VE EEEC
Sbjct: 39  RSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEEC 98

Query: 67  KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRW 126
           KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRW
Sbjct: 99  KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRW 158

Query: 127 GTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGEN 186
           GTPSATSITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLAEILGEN
Sbjct: 159 GTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGEN 218

Query: 187 LGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVK 246
           LGRSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVK
Sbjct: 219 LGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVK 278

Query: 247 DGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVI 306
           DGKWIAVKPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCPWSETVI
Sbjct: 279 DGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVI 338

Query: 307 KSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           KSKSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 339 KSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

[Close]

BLAST of PI0014446 vs. ExPASy TrEMBL
Match: A0A5A7UM12 (Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006030 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.4e-183
Identity = 318/332 (95.78%), Postives = 323/332 (97.29%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRA 73
           MDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+VE EEECKNDIIRA
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 60

Query: 74  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGTPSATS 133
           SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRWGTPSATS
Sbjct: 61  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 120

Query: 134 ITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKF 193
           ITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLAEILGENLGRSSKF
Sbjct: 121 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 180

Query: 194 FVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 253
           FVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV
Sbjct: 181 FVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 240

Query: 254 KPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSEPG 313
           KPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCPWSETVIKSKSEPG
Sbjct: 241 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 300

Query: 314 VYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           VYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

[Close]

BLAST of PI0014446 vs. ExPASy TrEMBL
Match: S0CRC1 (GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.4e-176
Identity = 311/337 (92.28%), Postives = 317/337 (94.07%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAA----AKGKSDVV--TVEEWDLPVVDLGRLTAGEVEAEEECKN 73
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLTAG+VE  E+CKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGT 133
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 134 PSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENLG 193
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEE+AGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 194 RSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253
           RSSKFFVENCVPSTCYLRMNRYPPC VPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 254 KWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 313
           KWIAVKPNPQALIINIGDLFQ WSND YKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 314 KSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           KSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

[Close]

BLAST of PI0014446 vs. ExPASy TrEMBL
Match: A0A6J1F422 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.2e-168
Identity = 298/345 (86.38%), Postives = 318/345 (92.17%), Query Frame = 0


Query: 1   MTQTLNQSSANVAMDPPFHEAYKTLLAETAAAKGKSDVVTVEEWDLPVVDLGRLTAG-EV 60
           M  TLNQ SANVAMDPPFHEAYK+LLAETA  +G  D V V+EW+LPVVDL RL  G EV
Sbjct: 1   MIHTLNQ-SANVAMDPPFHEAYKSLLAETAVERG-GDAVAVKEWELPVVDLRRLRDGAEV 60

Query: 61  EAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFS 120
              E+CKNDIIRAS+EWGFFQVVNHG+S+QLL KMRAKQI LFKQPFERKSKED FLNFS
Sbjct: 61  GEVEQCKNDIIRASQEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFS 120

Query: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLA 180
           AGSYRWGTP+ATS+TQLSWSEAFHVSLSDILGSN    DDLRSTMEEFAGKVSRLAQ+LA
Sbjct: 121 AGSYRWGTPTATSLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQELA 180

Query: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENLGRSSKFFV+NCVPSTCYLRMNRYPPC VPG++FGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCP 300
           GLELVKDG+WIAVKPNP+ALIINIGDLFQAWSN+VYKSVEHRVVTNSK ER+SIAYF CP
Sbjct: 241 GLELVKDGEWIAVKPNPEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCP 300

Query: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           WSETVIKSK EPGVYRRFSFREFRNQVQEDVRKHG+KIGLP+FVL
Sbjct: 301 WSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of PI0014446 vs. NCBI nr
Match: XP_008441451.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 666.4 bits (1718), Expect = 1.4e-187
Identity = 328/345 (95.07%), Postives = 333/345 (96.52%), Query Frame = 0


Query: 1   MTQTLNQSSANVAMDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEV 60
           MT  L QSSANVAMDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+V
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  EAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFS 120
           E EEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLA 180
           AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCP 300
           GLELVKDGKWIAVKPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

[Close]

BLAST of PI0014446 vs. NCBI nr
Match: XP_008441442.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 659.8 bits (1701), Expect = 1.3e-185
Identity = 324/339 (95.58%), Postives = 330/339 (97.35%), Query Frame = 0


Query: 7   QSSANVAMDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEEC 66
           +SSANVAMDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+VE EEEC
Sbjct: 39  RSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEEC 98

Query: 67  KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRW 126
           KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRW
Sbjct: 99  KNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRW 158

Query: 127 GTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGEN 186
           GTPSATSITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLAEILGEN
Sbjct: 159 GTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGEN 218

Query: 187 LGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVK 246
           LGRSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVK
Sbjct: 219 LGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVK 278

Query: 247 DGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVI 306
           DGKWIAVKPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCPWSETVI
Sbjct: 279 DGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVI 338

Query: 307 KSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           KSKSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 339 KSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

[Close]

BLAST of PI0014446 vs. NCBI nr
Match: KAA0056882.1 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa] >TYJ99385.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 318/332 (95.78%), Postives = 323/332 (97.29%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAA-AKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRA 73
           MDPPFHEAYKTLLAETAA AKG SDVVTVEEWDLPVVDLGRLT G+VE EEECKNDIIRA
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 60

Query: 74  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGTPSATS 133
           SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRWGTPSATS
Sbjct: 61  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 120

Query: 134 ITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKF 193
           ITQLSWSEAFHVSLSDILGSN GSDDDLRSTMEE+AGKVSRLAQKLAEILGENLGRSSKF
Sbjct: 121 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 180

Query: 194 FVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 253
           FVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV
Sbjct: 181 FVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 240

Query: 254 KPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSEPG 313
           KPNPQALI+NIGDLFQ WSND YKSVEHRVVTNS+KERYSIAYFLCPWSETVIKSKSEPG
Sbjct: 241 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 300

Query: 314 VYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           VYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

[Close]

BLAST of PI0014446 vs. NCBI nr
Match: XP_004153456.2 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >XP_031742868.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >KAE8652673.1 hypothetical protein Csa_013900 [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 2.7e-180
Identity = 322/351 (91.74%), Postives = 329/351 (93.73%), Query Frame = 0


Query: 1   MTQTL-NQSSANVAMDPPFHEAYKTLLAETAA----AKGKSDVV--TVEEWDLPVVDLGR 60
           MT TL +QSSANVAMDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL R
Sbjct: 1   MTHTLKHQSSANVAMDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLER 60

Query: 61  LTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED 120
           LTAG+VE  E+CKNDII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED
Sbjct: 61  LTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKED 120

Query: 121 QFLNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSR 180
           QF NFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEE+AGKVSR
Sbjct: 121 QFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSR 180

Query: 181 LAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTIL 240
           LAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPC VPGQIFGLMPHTDSDFLTIL
Sbjct: 181 LAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTIL 240

Query: 241 HQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSI 300
           HQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQ WSND YKSVEHRVVTNSKKERYSI
Sbjct: 241 HQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSI 300

Query: 301 AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 AYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 351

[Close]

BLAST of PI0014446 vs. NCBI nr
Match: XP_031742874.1 (gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis sativus] >CCL98007.1 GA 2-oxidase, partial [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 7.0e-176
Identity = 311/337 (92.28%), Postives = 317/337 (94.07%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAA----AKGKSDVV--TVEEWDLPVVDLGRLTAGEVEAEEECKN 73
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLTAG+VE  E+CKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWGT 133
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQF NFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 134 PSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENLG 193
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEE+AGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 194 RSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253
           RSSKFFVENCVPSTCYLRMNRYPPC VPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 254 KWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 313
           KWIAVKPNPQALIINIGDLFQ WSND YKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 314 KSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
           KSEPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

[Close]

BLAST of PI0014446 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.2e-120
Identity = 210/339 (61.95%), Postives = 255/339 (75.22%), Query Frame = 0


Query: 14  MDPPFHEAYKTLLAETAAAKGK---------SDVVTVEEWDLPVVDLGRLTAGEVEAEEE 73
           MDPPF+E Y  LL      K           S    VEE +LPV+D+ RL  G  E  E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 74  CKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYR 133
           CK  I RAS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 134 WGTPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGE 193
           WGTPSATSI QLSWSEAFHV ++DI  + D +   L STME+FA +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDISDNKDFT--TLSSTMEKFASESEALAYMLAEVLAE 180

Query: 194 NLGRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELV 253
             G++S FF ENCV +TCYLRMNRYPPCP P +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 254 KDGKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETV 313
           KD +WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN K ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 314 IKSKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFV 344
           I+  S+   YR FSFREFR QVQEDV+K G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of PI0014446 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 295.8 bits (756), Expect = 4.5e-80
Identity = 149/338 (44.08%), Postives = 218/338 (64.50%), Query Frame = 0


Query: 12  VAMDPPFHEAYKTLLA-----ETAAAKGKSDVVTVEEWDLPVVDLGRLTAGEVEAEEECK 71
           +A  PPF   + ++        T+ +   +  +      LPV+DL  LT+GE    + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 72  NDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFLNFSAGSYRWG 131
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +F + S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 132 TPSATSITQLSWSEAFHVSLSDILGSNDGSDDDLRSTMEEFAGKVSRLAQKLAEILGENL 191
            PSATS  Q S SEAFH+ LS++   +D   ++LR+ +E +  +++R+AQ + EILG+ +
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEVSRISD-DRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 192 GRSSKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKD 251
             SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  +
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 252 GKWIAVKPNPQALIINIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIK 311
           G+WI+VKP  +AL +NIGD+FQA SN VY+SV HRV++ +  ER SIA+F+CP+ ET I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 312 SKSEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFVL 345
               P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of PI0014446 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 3.0e-44
Identity = 119/334 (35.63%), Postives = 174/334 (52.10%), Query Frame = 0


Query: 38  VVTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAK 97
           +V  ++ ++PV+DL RL           K  +  A KEWG F++ NHG+   L ++++  
Sbjct: 26  IVQGKDINIPVIDLERLD----------KEILREACKEWGIFRLENHGVPLALTSRLQEI 85

Query: 98  QIELFKQPFERKSKEDQFLNFSAGSYRWGTPS----------ATSITQLSWSEAFHVSLS 157
              L   PFE+K +    +  S  SY WGTP+              + L+  E F+V LS
Sbjct: 86  SESLLSLPFEKKRELFAAVK-SPLSYFWGTPALNRSGDALKRGAQASNLTMLEGFNVPLS 145

Query: 158 DI-----LGSNDGSDDD---------LRSTMEEFAGKVSRLAQKLAEILGE--NLGRSSK 217
            +     L ++   DDD          R  MEE+   ++R+A  L E + +  NL  S  
Sbjct: 146 SLSSLSKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGN 205

Query: 218 FFVENCVPSTCYLRMNRYPPC--PVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKW 277
              E    ST  +R+ RYP        +  G+  HTDS  ++IL +D+ GGLE++K  +W
Sbjct: 206 RRSEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEIMKGEEW 265

Query: 278 IAVKPNPQALIINIGDLFQAWSNDVYKSVEHRV-VTNSKKERYSIAYFLCPWSETVIKSK 337
             VKP    LI+N+GD+ QA S+D YKSV HRV   N K ER+S+ YF+ P  + VIKS 
Sbjct: 266 FCVKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKSS 325

Query: 338 SEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 343
           +    Y+ F++ +F  QVQ DV+  G KIGLPRF
Sbjct: 326 N----YKLFTYSDFEAQVQADVQSLGTKIGLPRF 344

[Close]

BLAST of PI0014446 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 99/289 (34.26%), Postives = 143/289 (49.48%), Query Frame = 0


Query: 41  VEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIE 100
           V+   +P++DL    +G+     E    + +A+ + GFF + NHG+   LL++       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 101 LFKQPFERKSKEDQFLNFSAGSYRWGTPS--ATSI-----TQLSWSEAFHVSLS------ 160
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 161 -----DILGSNDGSD-DDLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKFFVENCVPST 220
                D +    G   +D     +E+A  ++ L+ K+ E+LG +LG   ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 221 CYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALII 280
              R+N YP C  P    G  PH D   LTILHQDQVGGL++  D KW ++ PNP A ++
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 281 NIGDLFQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 311
           NIGD F A +N  YKS  HR V NS++ER + A+FLCP  E V+K   E
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEE 332

[Close]

BLAST of PI0014446 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.1e-41
Identity = 97/284 (34.15%), Postives = 144/284 (50.70%), Query Frame = 0


Query: 39  VTVEEWDLPVVDLGRLTAGEVEAEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQ 98
           + V E D+P++DL  L + +  +  +    I  A K+ GFF VVNHGIS +L++      
Sbjct: 54  INVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYT 113

Query: 99  IELFKQPFERKSKEDQFLNFSAGSYRWGTP-SATSITQLSWSEAFHVSLSDILGSNDGSD 158
              F  P    S++ + L  S  S  + +  +    T+L W E       D +  +    
Sbjct: 114 SRFFDMPL---SEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQ 173

Query: 159 D-----------DLRSTMEEFAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRM 218
           D                 +E+   +S L+ K+ E+LG +LG    +F E    +   +R+
Sbjct: 174 DYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRL 233

Query: 219 NRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDL 278
           N YPPC  P    G  PH D   LTILHQD V GL++  + +W +++PNP+A ++NIGD 
Sbjct: 234 NYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDT 293

Query: 279 FQAWSNDVYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 311
           F A SND YKS  HR V NS+ ER S+A+FLCP  + V+    E
Sbjct: 294 FMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRE 333

The following BLAST results are available for this feature:

BLAST of PI0014446 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	1.6e-119	61.95	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q7XP65	1.4e-91	49.30	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	4.2e-83	48.21	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	6.3e-79	44.08	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q39112	2.3e-41	34.26	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1	[more]

BLAST of PI0014446 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B309	6.6e-188	95.07	gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A1S3B3F3	6.1e-186	95.58	gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A5A7UM12	6.4e-183	95.78	Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
S0CRC1	3.4e-176	92.28	GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1	[more]
A0A6J1F422	1.2e-168	86.38	gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 P...	[more]

BLAST of PI0014446 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441451.1	1.4e-187	95.07	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo]	[more]
XP_008441442.1	1.3e-185	95.58	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo]	[more]
KAA0056882.1	1.3e-182	95.78	gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa] >TYJ99385...	[more]
XP_004153456.2	2.7e-180	91.74	gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >XP_031742868.1 gi...	[more]
XP_031742874.1	7.0e-176	92.28	gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis sativus] >CCL98007.1 GA 2-o...	[more]

BLAST of PI0014446 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	1.2e-120	61.95	gibberellin 2-oxidase 8	[more]
AT1G50960.1	4.5e-80	44.08	gibberellin 2-oxidase 7	[more]
AT5G58660.1	3.0e-44	35.63	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G07200.1	1.7e-42	34.26	gibberellin 20-oxidase 3	[more]
AT4G25420.1	3.1e-41	34.15	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 46..147 e-value: 9.5E-16 score: 58.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 205..299 e-value: 9.3E-30 score: 103.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 44..337 e-value: 2.7E-81 score: 275.3
None	No IPR available	PANTHER	PTHR47990:SF13	GIBBERELLIN 2-BETA-DIOXYGENASE 8-LIKE	coord: 27..338
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 27..338
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 40..328
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 200..299 score: 15.63971

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014446.1	PI0014446.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009685	gibberellin metabolic process
molecular_function	GO:0052635	C-20 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding