PI0014408 (gene) Melon (PI 482460) v1

Overview


Name	PI0014408
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	gibberellin 20-oxidase-like protein
Location	chr07: 3276314 .. 3278272 (-)
RNA-Seq Expression	PI0014408
Synteny	PI0014408

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCCAGTGTAATTCCCAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGGTTGAGATGTACGAAGAATTAGCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAATCTGTACAGGGCGTGTAATGAATGGGGGCATTTCTACATCAAAAACCATGGTGTTTCGAACGAGCTGTACTGGAAACTCAGAGCCGTTACAGAGGAACTGTTGACGGCGGTGCCGGAAGAGACGAAGGAAGGGAAATTGAAAGTAGGAGTTGCTTGGTACACTCCTAGATTTAAAGTGTCGCCTTACATTGAGAGCTTCAAGTTTTTGGGACCGAATTTTTCAGATTCTGCCTCCGATGTAGGGTTTACGGAGCAAGTCTTCGGCCACCGCGTCACTCAATTCAGGTCATCCTAAACTCTATTTCAATTTCAAATCCTCCCAGATTCAATTATAATAAAATAACAAATTTGATTAAAGTTTTGGTCTTATCTGTACAAAACTCTCTTCAACACTTTCTATAGTTTAAGAAAATTAACATTTTTAAATATAGCAAATTGGACCAAAATATAAAATATTCTCAACATTTTTTCATTTAAAAAATTTGTATCATATTCATAAATGAAATAATATTTTAAGATTTCAAATTACATATTTACAATCATTTTTGAAACTTTTTCCGTTAATATGATGGAGAAACTTACTCTCTCTTAACACTATCAATGAATTTTGTTTGAAAGAATGTGGACACTGAAAAGTTTGAGAACTTGAGTTATCTCTTAAGAATTATTAGGATATATTTATAATATAGTTTGACATAGATCTTTTCACGTCTCCTTAAAAAACAATGTTATCAAGTCACCTAGTTAGATATTTTGTGGGCTTATTAGATTGATGTGTGGTATGTTGCGCATGTTCGATGTGCAATACTCCAATATGTCTTGTCTTGTCCTCGATCTAGTTTCTTATCCTTTTAACTTTAGATTTATCATTCTAGTTTAAAATTATACATAACATAGTTCATAAATTTATTATACAATTAAGAGAACTAAATGAAAGTATATAAAAACTTATTGGAACGTTTTAAAATACATGGAAATGGTTTTCTTTATTTTATGAATTGTTTTGTTTTCTTTGTTTCTTAAGAAAATTTGTTTGCAGGAATCTACTTGATGAATATGGAAGTATAATGTTAGAACTATCAAGAAGAATAACGAAGCTATTACTAAAGATCATGGGAGATACTTTTGAGGACAAATTCTATGAATCTGAATTCAATAACTGCAATGGCTACATAAGAATAAACAGGTATGCACCTCGAAACTCCGATCAAGAAACCGAAGCGTTCGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATAGGAGGTCTTCAAATGAAATCGAAACAAGGAGACGGGTGGATGGATGTAAGACCTCTAAAGGATGCCCTTTTAGTGAACATTGGGGATTTTTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTGGGGATGATGATAGGGAAATATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGGCTTTATAAGCCATTTTCTACTAAAGAATACAGGGCATTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATTAGAGAATTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGCATCCACGTTTGAAATGATAATAGTATTCATTCTTCTTGTATTAGAATTGGTTGGTGACTCATACTGGCCATTCTCCTGTATCATAAAATATATATTTTAATTTTTAACTTTCAATTTGCTCATCTCAATTCATAAACTTGGAACAAAAAACAACTATGTTACTAACACTTCTTAAGTGTATGTTAGAT

mRNA sequence

AAGCCAGTGTAATTCCCAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGGTTGAGATGTACGAAGAATTAGCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAATCTGTACAGGGCGTGTAATGAATGGGGGCATTTCTACATCAAAAACCATGGTGTTTCGAACGAGCTGTACTGGAAACTCAGAGCCGTTACAGAGGAACTGTTGACGGCGGTGCCGGAAGAGACGAAGGAAGGGAAATTGAAAGTAGGAGTTGCTTGGTACACTCCTAGATTTAAAGTGTCGCCTTACATTGAGAGCTTCAAGTTTTTGGGACCGAATTTTTCAGATTCTGCCTCCGATGTAGGGTTTACGGAGCAAGTCTTCGGCCACCGCGTCACTCAATTCAGGAATCTACTTGATGAATATGGAAGTATAATGTTAGAACTATCAAGAAGAATAACGAAGCTATTACTAAAGATCATGGGAGATACTTTTGAGGACAAATTCTATGAATCTGAATTCAATAACTGCAATGGCTACATAAGAATAAACAGGTATGCACCTCGAAACTCCGATCAAGAAACCGAAGCGTTCGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATAGGAGGTCTTCAAATGAAATCGAAACAAGGAGACGGGTGGATGGATGTAAGACCTCTAAAGGATGCCCTTTTAGTGAACATTGGGGATTTTTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTGGGGATGATGATAGGGAAATATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGGCTTTATAAGCCATTTTCTACTAAAGAATACAGGGCATTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATTAGAGAATTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGCATCCACGTTTGAAATGATAATAGTATTCATTCTTCTTGTATTAGAATTGGTTGGTGACTCATACTGGCCATTCTCCTGTATCATAAAATATATATTTTAATTTTTAACTTTCAATTTGCTCATCTCAATTCATAAACTTGGAACAAAAAACAACTATGTTACTAACACTTCTTAAGTGTATGTTAGAT

Coding sequence (CDS)

ATGTCAAAAGAATCGGTTGAGATGTACGAAGAATTAGCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAATCTGTACAGGGCGTGTAATGAATGGGGGCATTTCTACATCAAAAACCATGGTGTTTCGAACGAGCTGTACTGGAAACTCAGAGCCGTTACAGAGGAACTGTTGACGGCGGTGCCGGAAGAGACGAAGGAAGGGAAATTGAAAGTAGGAGTTGCTTGGTACACTCCTAGATTTAAAGTGTCGCCTTACATTGAGAGCTTCAAGTTTTTGGGACCGAATTTTTCAGATTCTGCCTCCGATGTAGGGTTTACGGAGCAAGTCTTCGGCCACCGCGTCACTCAATTCAGGAATCTACTTGATGAATATGGAAGTATAATGTTAGAACTATCAAGAAGAATAACGAAGCTATTACTAAAGATCATGGGAGATACTTTTGAGGACAAATTCTATGAATCTGAATTCAATAACTGCAATGGCTACATAAGAATAAACAGGTATGCACCTCGAAACTCCGATCAAGAAACCGAAGCGTTCGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATAGGAGGTCTTCAAATGAAATCGAAACAAGGAGACGGGTGGATGGATGTAAGACCTCTAAAGGATGCCCTTTTAGTGAACATTGGGGATTTTTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTGGGGATGATGATAGGGAAATATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGGCTTTATAAGCCATTTTCTACTAAAGAATACAGGGCATTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATTAGAGAATTTGCTGGAATTGATTTGGAGTGA

Protein sequence

MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFRENNYGKIVGTPIREFAGIDLE

Homology

[Close]

BLAST of PI0014408 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.6e-68
Identity = 149/330 (45.15%), Postives = 202/330 (61.21%), Query Frame = 0


Query: 11  ELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEE 70
           EL V D++  + LSESS  +L  AC EWG FY+ NHGVS ++Y KLR  +    T V E 
Sbjct: 4   ELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGVFEL 63

Query: 71  TKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRVT--QFRNLL 130
             E K+K+G + YTPRF  SP+ ES +  GP+F  SA     +   F  + T  +F  L+
Sbjct: 64  EDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLM 123

Query: 131 DEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYA--------PRNS 190
            EYG  M +L  +I K +L   GD    K+YESEF NC+GY RIN Y           N 
Sbjct: 124 KEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNG 183

Query: 191 DQE--TEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAW 250
           D++   E  G HTD+SC+TI+ QD+IGGLQ++++ G G MD+ P  +AL+VN+GD L AW
Sbjct: 184 DEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAW 243

Query: 251 SNERLRSAEHRVVLKQN---VKRFSLAFFLIFGDDDREIYAPSEVVG--EGNTRLYKPFS 310
           +N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++ F 
Sbjct: 244 TNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFK 303

Query: 311 TKEYRAFRENN-YGKI--VGTPIREFAGID 321
             +Y  FRE+N  GK   VG  + +FA I+
Sbjct: 304 CGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of PI0014408 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.1e-28
Identity = 92/292 (31.51%), Postives = 136/292 (46.58%), Query Frame = 0


Query: 33  RACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEETKE--GKLKVGVAWYTPRFKVS 92
           RA +EWG F + NHGV  EL  ++R   +  L  +P ETKE  G L     W  P     
Sbjct: 75  RAASEWGFFQLTNHGVGRELMEEMRR-EQARLFRLPFETKEKAGLLNGSYRWGNPTATSL 134

Query: 93  PYIE-SFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDEYGSIMLELSRRITKLLLKI 152
            ++  S  F  P  S S +D  F +      +T  R ++ E    M  ++  +   L + 
Sbjct: 135 RHLSWSEAFHVPLASISGADCDFGD------LTSLRGVMQEVAEAMSRVANTVAAALAEE 194

Query: 153 M-----GDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKHTDISCVTILFQDEI 212
           +     G      ++ +  +    ++R+NRY       +T     HTD   +T+L QD++
Sbjct: 195 LTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQDQV 254

Query: 213 GGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRVVLKQNVKRFSLAFF 272
           GGL +   +   W+ VRP  DAL+VNIGD  QAWSN R +S EH+VV      R S+A+F
Sbjct: 255 GGLHL--MKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSVAYF 314

Query: 273 LIFGDDDREIYAPSEVVGE-GNTRLYKPFSTKEYRAFRENNY---GKIVGTP 313
           L    D         +VG  G    Y+ F+  EYR   + +    GK +G P
Sbjct: 315 LCPSYD--------SLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLP 349

[Close]

BLAST of PI0014408 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 88/314 (28.03%), Postives = 146/314 (46.50%), Query Frame = 0


Query: 11  ELAVMD----MNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELL-T 70
           EL V+D    ++  E   E  +  + RA  EWG F + NHG+S ++  K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEETKEGKLKVG-VAWYTP---RFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRV 130
              +++K  K   G   W TP     +   + E+F     + SD+               
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKD------------F 160

Query: 131 TQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSD 190
           T   + ++++ S    L+  + ++L +  G      F++        Y+R+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQ--NSSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 QETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNE 250
            E      HTD   +TIL+QD++GGLQ+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENN---YGKIVGTP 313
           + +   +G  VG P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]

BLAST of PI0014408 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.4e-26
Identity = 86/278 (30.94%), Postives = 123/278 (44.24%), Query Frame = 0


Query: 34  ACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEETKEGKLKVG-----VAWYTPRFK 93
           AC   G F + NHG+  EL        +   T    E +    + G      + +T RF 
Sbjct: 65  ACERHGFFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFA 124

Query: 94  VS-PYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDEYGSIMLELSRRITKLLL 153
              P+ E+  F     SD A  V +     G    +   +   Y S M  LS  I ++L 
Sbjct: 125 SKLPWKETLSFRSCP-SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLG 184

Query: 154 KIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKHTDISCVTILFQDEIGGL 213
           + +G       Y   F   +  +R+N Y P     ET   G H D + +TIL QD +GGL
Sbjct: 185 ESLG--VGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGL 244

Query: 214 QMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRVVLKQNVKRFSLAFFLIF 273
           Q+ ++    W  +RP  DA +VNIGD   A SN R +S  HR V+   V R SLAFFL  
Sbjct: 245 QVHTE--GRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLC- 304

Query: 274 GDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFRENNY 306
            + D+ +  P  +V   N R Y  F+ +    F + +Y
Sbjct: 305 PEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHY 336

[Close]

BLAST of PI0014408 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.7e-26
Identity = 86/278 (30.94%), Postives = 123/278 (44.24%), Query Frame = 0


Query: 34  ACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEETKEGKLKVG-----VAWYTPRFK 93
           AC   G F + NHG+  EL        +   T    E +    + G      + +T RF 
Sbjct: 65  ACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFA 124

Query: 94  VS-PYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDEYGSIMLELSRRITKLLL 153
              P+ E+  F     SD A  V +     G    +   +   Y S M  LS  I ++L 
Sbjct: 125 SKLPWKETLSFRSCP-SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLG 184

Query: 154 KIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKHTDISCVTILFQDEIGGL 213
           + +G       Y   F   +  +R+N Y P     ET   G H D + +TIL QD +GGL
Sbjct: 185 ESLG--VGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGL 244

Query: 214 QMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRVVLKQNVKRFSLAFFLIF 273
           Q+ ++    W  +RP  DA +VNIGD   A SN R +S  HR V+   V R SLAFFL  
Sbjct: 245 QVHTE--GRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLC- 304

Query: 274 GDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFRENNY 306
            + D+ +  P  +V   N R Y  F+ +    F + +Y
Sbjct: 305 PEMDKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHY 336

[Close]

BLAST of PI0014408 vs. ExPASy TrEMBL
Match: A0A5D3D2E7 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00730 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.5e-170
Identity = 286/322 (88.82%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM EEL VMDMNCCEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEE KE KLKVGV+WYTPRFK+SPY ESFKFLGP FSDSASD+GFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIM+ELSRRI KLLLK+MGD+FEDKFYESEF+NCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGG+QMKSKQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQN KRF+L FFL+FGDDDRE+YAPSEVVGEGN R+YKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNYGK+VG P+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. ExPASy TrEMBL
Match: A0A1S3BYJ4 (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.5e-170
Identity = 286/322 (88.82%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM EEL VMDMNCCEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEE KE KLKVGV+WYTPRFK+SPY ESFKFLGP FSDSASD+GFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIM+ELSRRI KLLLK+MGD+FEDKFYESEF+NCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGG+QMKSKQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQN KRF+L FFL+FGDDDRE+YAPSEVVGEGN R+YKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNYGK+VG P+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. ExPASy TrEMBL
Match: A0A5A7TME4 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004080 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.3e-169
Identity = 284/322 (88.20%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM EEL VMDMNCCEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEE KE KLKVGV+WYTPRFK+SPY ESFKFLGP FSDSASD+GFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIM+ELSRRI KLLLK+MGD+FEDKFYESEF+NCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGG+QMKSKQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRS EHRVVLKQN +RF+L FFL+FGDDDRE+YAPSEVVGEGN R+YKPFSTKEYRAF
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNYGK+VG P+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. ExPASy TrEMBL
Match: A0A0A0KW97 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 281/322 (87.27%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM+EEL VMDMN CEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELY KLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEETKEGKLKVG +WYTPRF++SPYIESFKFLGPNFSD ASD+GFTEQVFG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRI KLLLK MGD  EDKFYESEF+NCNGY+RINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTI+FQDEIGG+QMK KQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQ+VKRFSLAFFLIF DDDRE+YAPSEVVGEGNTR+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNY KIVG P+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.2e-103
Identity = 196/313 (62.62%), Postives = 244/313 (77.96%), Query Frame = 0


Query: 11  ELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEE 70
           EL V+D++  + LSES   +L  ACNEWG FYI NHGVS+E Y KL ++ +EL   +P++
Sbjct: 9   ELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-RLPQD 68

Query: 71  TKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDE 130
           T   K+KVG++ Y PRF  SP+ ESFK  GP+FS SA  +  TE + GH  TQF NLL+E
Sbjct: 69  T---KIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLL--TEALLGHHNTQFSNLLEE 128

Query: 131 YGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKH 190
           YGS M +LS+RI KLLLK++GD FE+KFY+ EF NC+GY+RIN Y+PR+S++E E  GKH
Sbjct: 129 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 188

Query: 191 TDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRV 250
           TDISCVTILFQD++GGLQM+SK G+ WMD+RP ++AL+VNIGDF+QAWSNERLRSAEHRV
Sbjct: 189 TDISCVTILFQDKLGGLQMRSKGGE-WMDIRPCENALVVNIGDFMQAWSNERLRSAEHRV 248

Query: 251 VLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFR----ENNYG 310
           VL+QNVKRFS+AFF  + DD+ EIYAPSEVVGEGN RLY+ FSTKEYR FR       Y 
Sbjct: 249 VLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYE 308

Query: 311 KIVGTPIREFAGI 320
           K VG  ++EFAGI
Sbjct: 309 K-VGANVKEFAGI 310

[Close]

BLAST of PI0014408 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 608.6 bits (1568), Expect = 3.1e-170
Identity = 286/322 (88.82%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM EEL VMDMNCCEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEE KE KLKVGV+WYTPRFK+SPY ESFKFLGP FSDSASD+GFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIM+ELSRRI KLLLK+MGD+FEDKFYESEF+NCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGG+QMKSKQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQN KRF+L FFL+FGDDDRE+YAPSEVVGEGN R+YKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNYGK+VG P+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. NCBI nr
Match: KAA0044653.1 (gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 605.5 bits (1560), Expect = 2.6e-169
Identity = 284/322 (88.20%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM EEL VMDMNCCEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEE KE KLKVGV+WYTPRFK+SPY ESFKFLGP FSDSASD+GFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIM+ELSRRI KLLLK+MGD+FEDKFYESEF+NCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGG+QMKSKQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRS EHRVVLKQN +RF+L FFL+FGDDDRE+YAPSEVVGEGN R+YKPFSTKEYRAF
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNYGK+VG P+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. NCBI nr
Match: XP_004146911.1 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_014948 [Cucumis sativus])

HSP 1 Score: 592.4 bits (1526), Expect = 2.3e-165
Identity = 281/322 (87.27%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MSKES+EM+EEL VMDMN CEHLSESSRL+LY+ACNEWGHFYIKNHGVS ELY KLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLTAVPEETKEGKLKVG +WYTPRF++SPYIESFKFLGPNFSD ASD+GFTEQVFG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRI KLLLK MGD  EDKFYESEF+NCNGY+RINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTI+FQDEIGG+QMK KQGD W+DVRPL+DALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQ+VKRFSLAFFLIF DDDRE+YAPSEVVGEGNTR+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYGKIVGTPIREFAGIDLE 323
           RENNY KIVG P+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]

BLAST of PI0014408 vs. NCBI nr
Match: XP_038876311.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 565.5 bits (1456), Expect = 3.0e-157
Identity = 272/318 (85.53%), Postives = 295/318 (92.77%), Query Frame = 0


Query: 1   MSKESVEMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVT 60
           MS+ES+EMYEEL VMD++CC+ LSESSR  LY+ACNEWGHFYI+NHGVS ELY KLRAVT
Sbjct: 1   MSEESIEMYEELPVMDIHCCDDLSESSRSCLYKACNEWGHFYIRNHGVSKELYLKLRAVT 60

Query: 61  EELLTAVPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHR 120
           +ELLT   EE KEGKLK GV+WYTPRF +SPYIESFKFLGPNFS SASD+GFTEQVFG R
Sbjct: 61  DELLT---EERKEGKLKEGVSWYTPRFILSPYIESFKFLGPNFSASASDLGFTEQVFGQR 120

Query: 121 VTQFRNLLDEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNS 180
           VTQ  NLLDEYGSIM+ELSR+I KLLLKIMGD  E+KFYESEF+ CNGYIRINRYAPRNS
Sbjct: 121 VTQLSNLLDEYGSIMIELSRKIMKLLLKIMGDNLENKFYESEFSKCNGYIRINRYAPRNS 180

Query: 181 DQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSN 240
           ++E EAFGKHTDISCVTILFQDEIGGLQMKSKQGD W+DV+PL+DALLVNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGLQMKSKQGDEWIDVKPLEDALLVNIGDFMQAWSN 240

Query: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300

Query: 301 RENNYGKIVGTPIREFAG 319
           RE NYGK+VGTP+REFAG
Sbjct: 301 REYNYGKVVGTPLREFAG 315

[Close]

BLAST of PI0014408 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 386.0 bits (990), Expect = 3.3e-103
Identity = 196/313 (62.62%), Postives = 244/313 (77.96%), Query Frame = 0


Query: 11  ELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEE 70
           EL V+D++  + LSES   +L  ACNEWG FYI NHGVS+E Y KL ++ +EL   +P++
Sbjct: 9   ELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-RLPQD 68

Query: 71  TKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNLLDE 130
           T   K+KVG++ Y PRF  SP+ ESFK  GP+FS SA  +  TE + GH  TQF NLL+E
Sbjct: 69  T---KIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLL--TEALLGHHNTQFSNLLEE 128

Query: 131 YGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYAPRNSDQETEAFGKH 190
           YGS M +LS+RI KLLLK++GD FE+KFY+ EF NC+GY+RIN Y+PR+S++E E  GKH
Sbjct: 129 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 188

Query: 191 TDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLRSAEHRV 250
           TDISCVTILFQD++GGLQM+SK G+ WMD+RP ++AL+VNIGDF+QAWSNERLRSAEHRV
Sbjct: 189 TDISCVTILFQDKLGGLQMRSKGGE-WMDIRPCENALVVNIGDFMQAWSNERLRSAEHRV 248

Query: 251 VLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFR----ENNYG 310
           VL+QNVKRFS+AFF  + DD+ EIYAPSEVVGEGN RLY+ FSTKEYR FR       Y 
Sbjct: 249 VLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYE 308

Query: 311 KIVGTPIREFAGI 320
           K VG  ++EFAGI
Sbjct: 309 K-VGANVKEFAGI 310

[Close]

BLAST of PI0014408 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 4.0e-75
Identity = 144/326 (44.17%), Postives = 210/326 (64.42%), Query Frame = 0


Query: 7   EMYEELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTA 66
           E++  L +  ++  + +  S   +L  AC EWG FY+ NHG+S E++ K+ +++ ++  A
Sbjct: 3   ELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA 62

Query: 67  VPEETKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSA---SDVGFTEQVFGHRVTQ 126
                 E KLK+G   YTPR+  SPY ES    GP+FSDSA   +DV F +    H   +
Sbjct: 63  ----PLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQD----HHKPE 122

Query: 127 FRNLLDEYGSIMLELSRRITKLLLKI-MGDTFEDKFYESEFNNCNGYIRINRYAP----R 186
            R  + EYG+ M ELS+R+ K+LL + +GD    + Y+++F+NC+GY+R+  Y P     
Sbjct: 123 LRETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVE 182

Query: 187 NSDQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAW 246
             ++  E  G HTD+SC+TI++QD +GGLQM+SK+G  W+D+ P  D L+VNIGD +QAW
Sbjct: 183 KQEELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGK-WIDINPCNDFLVVNIGDLMQAW 242

Query: 247 SNERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYR 306
           SN RLRS+EHRVVL++ V R SLAFFL F +D++ I AP E+VGEG  R YK F   EY 
Sbjct: 243 SNGRLRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYL 302

Query: 307 AFRENN-YGKI--VGTPIREFAGIDL 322
            FR++N  GK   +G  +++FAG+ L
Sbjct: 303 KFRQSNEEGKFEKIGFTVKDFAGLKL 318

[Close]

BLAST of PI0014408 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.5e-69
Identity = 149/330 (45.15%), Postives = 202/330 (61.21%), Query Frame = 0


Query: 11  ELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTAVPEE 70
           EL V D++  + LSESS  +L  AC EWG FY+ NHGVS ++Y KLR  +    T V E 
Sbjct: 4   ELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGVFEL 63

Query: 71  TKEGKLKVGVAWYTPRFKVSPYIESFKFLGPNFSDSASDVGFTEQVFGHRVT--QFRNLL 130
             E K+K+G + YTPRF  SP+ ES +  GP+F  SA     +   F  + T  +F  L+
Sbjct: 64  EDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLM 123

Query: 131 DEYGSIMLELSRRITKLLLKIMGDTFEDKFYESEFNNCNGYIRINRYA--------PRNS 190
            EYG  M +L  +I K +L   GD    K+YESEF NC+GY RIN Y           N 
Sbjct: 124 KEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNG 183

Query: 191 DQE--TEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAW 250
           D++   E  G HTD+SC+TI+ QD+IGGLQ++++ G G MD+ P  +AL+VN+GD L AW
Sbjct: 184 DEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAW 243

Query: 251 SNERLRSAEHRVVLKQN---VKRFSLAFFLIFGDDDREIYAPSEVVG--EGNTRLYKPFS 310
           +N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++ F 
Sbjct: 244 TNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFK 303

Query: 311 TKEYRAFRENN-YGKI--VGTPIREFAGID 321
             +Y  FRE+N  GK   VG  + +FA I+
Sbjct: 304 CGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of PI0014408 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 5.7e-53
Identity = 100/202 (49.50%), Postives = 142/202 (70.30%), Query Frame = 0


Query: 128 LDEYGSIMLELSRRITKLLLKI-MGDTFEDKFYESEFNNCNGYIRINRYAP----RNSDQ 187
           + EYG+ M ELS+R+ K+LL + +GD    + Y+++F+NC+GY+R+  Y P       ++
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 188 ETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNER 247
             E  G HTD+SC+TI++QD +GGLQM+SK+G  W+D+ P  D L+VNIGD +QAWSN R
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGK-WIDINPCNDFLVVNIGDLMQAWSNGR 120

Query: 248 LRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFRE 307
           LRS+EHRVVL++ V R SLAFFL F +D++ I AP E+VGEG  R YK F   EY  FR+
Sbjct: 121 LRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 180

Query: 308 NN-YGKI--VGTPIREFAGIDL 322
           +N  GK   +G  +++FAG+ L
Sbjct: 181 SNEEGKFEKIGFTVKDFAGLKL 200

[Close]

BLAST of PI0014408 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 7.4e-29
Identity = 82/314 (26.11%), Postives = 143/314 (45.54%), Query Frame = 0


Query: 10  EELAVMDMNCCE---HLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELLTA 69
           +E+  +D++  E   H   +    +  AC  WG F + NHG+ + L  ++     E    
Sbjct: 30  DEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 89

Query: 70  VPEETKEGKL----KVGVAWYTPRFKVSPYIESFKFL---------GPNFSDSASDVGFT 129
             EE ++ K      +G         V  + E F F           P   D  +++   
Sbjct: 90  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPED--TELRKL 149

Query: 130 EQVFGHRVTQFRNLLDEYGSIMLELSRRITKLL---LKIMGDTFEDKFYESEFNNCNGYI 189
              +    + FR +  EY   + +L+ R+ +L+   L + GD          FN    ++
Sbjct: 150 TNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRL-----TGFFNEQTSFL 209

Query: 190 RINRYAPRNSDQETEAFGKHTDISCVTILFQDEIGGLQMKSKQGDGWMDVRPLKDALLVN 249
           R N Y P  + +     G+H D   +T+L QD +GGLQ+  +    W+ V+P+ DAL++N
Sbjct: 210 RFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIIN 269

Query: 250 IGDFLQAWSNERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYK 305
           +G+ +Q W+N+   SAEHRVV+  + +RFS+ FF  F   +  I    E++ E N   YK
Sbjct: 270 MGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFF-FFPSHEANIEPLEELISEENPPCYK 329

[Close]

BLAST of PI0014408 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-25
Identity = 88/295 (29.83%), Postives = 141/295 (47.80%), Query Frame = 0


Query: 11  ELAVMDMNCCEHLSESSRLNLYRACNEWGHFYIKNHGVSNELYWKLRAVTEELL-TAVPE 70
           E+ V+DMN      E  RL +  AC EWG F + NHGV++ L  ++R    E     + E
Sbjct: 47  EIPVLDMNDVWGKPEGLRL-VRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEE 106

Query: 71  ETKEGKLKVGVAWYTPRFKV--SPYIESFKFLGPNFSDSASDVGFTEQVFGHRVTQFRNL 130
           + K          Y  R  V     ++   +   N+  S+         +  +  + R L
Sbjct: 107 KRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIR---NPSKWPSQPPKIREL 166

Query: 131 LDEYGSIMLELSRRITKLLLKIMGDTFEDKFYES--EFNNCNGYIRINRYAPRNSDQETE 190
           +++YG  + +L  R+T+ L + +G    +K  ++    +     +R N Y      Q T 
Sbjct: 167 IEKYGEEVRKLCERLTETLSESLG-LKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTL 226

Query: 191 AFGKHTDISCVTILFQDE-IGGLQMKSKQGDGWMDVRPLKDALLVNIGDFLQAWSNERLR 250
               H+D   +TIL  DE + GLQ+  ++GDGW+ ++ + +AL+VNIGD LQ  SN   +
Sbjct: 227 GLSSHSDPGGITILLPDEKVAGLQV--RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYK 286

Query: 251 SAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRA 300
           S EH+V++   ++R SLAFF      D  +    E+V      LYKP    EYR+
Sbjct: 287 SVEHQVIVNSGMERVSLAFF-YNPRSDIPVGPIEELVTANRPALYKPIRFDEYRS 333

The following BLAST results are available for this feature:

BLAST of PI0014408 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	3.6e-68	45.15	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q7XP65	2.1e-28	31.51	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
O49561	2.6e-26	28.03	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
O04706	4.4e-26	30.94	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
O04707	5.7e-26	30.94	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]

BLAST of PI0014408 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D2E7	1.5e-170	88.82	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BYJ4	1.5e-170	88.82	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
A0A5A7TME4	1.3e-169	88.20	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A0A0KW97	1.1e-165	87.27	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A6J1CI26	1.2e-103	62.62	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]

BLAST of PI0014408 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453897.1	3.1e-170	88.82	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberell...	[more]
KAA0044653.1	2.6e-169	88.20	gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa]	[more]
XP_004146911.1	2.3e-165	87.27	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical p...	[more]
XP_038876311.1	3.0e-157	85.53	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
XP_022141254.1	3.3e-103	62.62	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]

BLAST of PI0014408 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	4.0e-75	44.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	2.5e-69	45.15	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	5.7e-53	49.50	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	7.4e-29	26.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G38240.1	1.7e-25	29.83	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..265 e-value: 1.6E-21 score: 76.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..73 e-value: 7.1E-8 score: 33.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..305 e-value: 1.5E-60 score: 207.2
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 6..310
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..310
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 30..305
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..267 score: 11.313832

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014408.1	PI0014408.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding