PI0014402 (gene) Melon (PI 482460) v1

Overview
NamePI0014402
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description5-formyltetrahydrofolate cyclo-ligase
Locationchr02: 23953012 .. 23955382 (-)
RNA-Seq ExpressionPI0014402
SyntenyPI0014402
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAAATATCAGCTCCTCCAAGGAAACAGGACAAATTTACCGGTAAATGGATTTCAAAATTCGACCACTGGTTCTAGGTTGACGAACCAGCTAAGTCCAGCCGGAGTGCATTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTCCGAAGCCCCAATGCTCCGAAACTATAAACCATTGTTACCCATTGCAGCAAATTCACTGTTCAATCACATCCAATCTTCAGCTTTTCAACAATCCCTTCCTCCATTATCGCGTTTCTCATCTGGGTATCCAAAAATGGCGACAAACGAGAGTATCAAGCCAGAAAATGAAAACCAATTGAAACACATATTTGATCAGAAACGGGTTCTTCGATCCAGCGTTCGAAAGGCCCTCAAAGCCATGGACCCCTCTCTCCGATCCCAGGAAGGTATCGGTTGCTTACGTATATTAATCGATTACTAGTGAATTAGTTGATATGATATCTTTTTAAGATGTTTCTTAATCGACGAATGCGATTTTCTATTTTCCCTCTTTCATTGGGCTCTATACTTGTTTCTCAGACAATGTAATTCAAAGTATTGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAGTGCGTTAAGGGAGGTTGATACATCGAGACTATTATCAGAGATTCTTCAACACCCTCCTAAAGGTGTGTTCTTAAACTTATTAGCCGCTTACTCATTCATGCGGTTGATATATCTGAAGTTTCAAGGATTTGTTTGGCAACATTCACGCGAATATGTCAATAAAGCTCAATGAAAGATTTTATATTTGAAGTTTTAGATCAAATAAAAGGAACACAATTAGTCACTGATTCAATTAGATTCTTTAGATCAGTGTAATTATTAGTGTGGGAGAATGTAATCGTGGCTTGGAAATGGTTTCAATTTCAAGTAGAGAGACTATTTGACGTAGTGGATATGAATGCAGAGGACATATTGGTAGATTTGTTGCTCATGATACGGATAAGTTGTTCTTTGAACTCGATGTTGGCATCTCGATACTGAGATAATTCTCAGTTTTAATCGAGGAAGTTATTGATTATGGTATGGTTTGATTGAGCTTTGTAAATGTTGACATTTTTCTTTTTATCTGAAGATGGTTATTCGAAGAAGATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAATTCAATGAACATCCTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGGTAAATAAATACTCCAGCCCACCTCTTGTCCCTTTATTTTCTAGTATTTCACGTATGAAATTAGACTAATACTCAAAATACAAAGAGACTTACAATATAGAATCTTAGGATCAGGAGATACACCCAAATATCTCAATTAGGTTGACGCACCCTTAGCACCCTCATCACATCCAATACAAAACAAAAATTCTCTTACCATCTTGAATGCAACACCATTGATATTTTAACATTTATGTTTTCCAGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGTGAGATATGATATCTATTTGTTGGCTGTTTAGGGTTTATTTATAGGTCAGAAGCCAGCATTAAATTACTTTTTAATATGTTGATGATGATTGCTGCCAGGACTAGCATTTGACAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGGTATGGATTTGCATTTAACCTTTCTGAGGTTTCTTTCTGCTGCTGTTATTACTTAAACACATTTGGACCTGATTTGGGAAGTTGAAACTAGTAGAGTGACTCCCAAATCATGATAGTGAAATCTTTCCCTCTATTTGTTTTTTCTTTGTTTGTTTATATTTTGGCCTACAGTCAATCTGTATAATCTTATACGTCATACACTCCAGATGATTCTTATTCTATAATTGTTATGGAAACAGTTATTATGATACCTTCCTAAAGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGGTATGTGTGTAAGCTCGCTCTTTTCCAGTAGAAACTCACCTTAGTTTCTCTTTTACTCCACCTTGTTTCCCTGCAACTTCACTTAACTCATTTTGCATGATTTGCAGTTGCACTGTCCTACTCAGTGCAAATAATGGATGAAGGAATTATACCACTCACTCCAAACGATGTTTTAGTCGATGCTCTTGTATCACCGTCTGGTGTGATTCCCATCAGCTCAGCTGGATTGGACAAGTAATTTATTTAATCTGAGAAGTCTAACCGCTTGTCATTCCAACTTTTTTTTTATTTCTTACTTCCTACTTGTCATGTTCTCGCAGAATAAAAGTATGA

mRNA sequence

ATGGTTAAATATCAGCTCCTCCAAGGAAACAGGACAAATTTACCGGTAAATGGATTTCAAAATTCGACCACTGGTTCTAGGTTGACGAACCAGCTAAGTCCAGCCGGAGTGCATTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTCCGAAGCCCCAATGCTCCGAAACTATAAACCATTGTTACCCATTGCAGCAAATTCACTGTTCAATCACATCCAATCTTCAGCTTTTCAACAATCCCTTCCTCCATTATCGCGTTTCTCATCTGGGTATCCAAAAATGGCGACAAACGAGAGTATCAAGCCAGAAAATGAAAACCAATTGAAACACATATTTGATCAGAAACGGGTTCTTCGATCCAGCGTTCGAAAGGCCCTCAAAGCCATGGACCCCTCTCTCCGATCCCAGGAAGACAATGTAATTCAAAGTATTGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAGTGCGTTAAGGGAGGTTGATACATCGAGACTATTATCAGAGATTCTTCAACACCCTCCTAAAGATGGTTATTCGAAGAAGATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAATTCAATGAACATCCTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGACTAGCATTTGACAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAAGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGTTGCACTGTCCTACTCAGTGCAAATAATGGATGAAGGAATTATACCACTCACTCCAAACGATGTTTTAGTCGATGCTCTTGTATCACCGTCTGGTGTGATTCCCATCAGCTCAGCTGGATTGGACAAAATAAAAGTATGA

Coding sequence (CDS)

ATGGTTAAATATCAGCTCCTCCAAGGAAACAGGACAAATTTACCGGTAAATGGATTTCAAAATTCGACCACTGGTTCTAGGTTGACGAACCAGCTAAGTCCAGCCGGAGTGCATTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTCCGAAGCCCCAATGCTCCGAAACTATAAACCATTGTTACCCATTGCAGCAAATTCACTGTTCAATCACATCCAATCTTCAGCTTTTCAACAATCCCTTCCTCCATTATCGCGTTTCTCATCTGGGTATCCAAAAATGGCGACAAACGAGAGTATCAAGCCAGAAAATGAAAACCAATTGAAACACATATTTGATCAGAAACGGGTTCTTCGATCCAGCGTTCGAAAGGCCCTCAAAGCCATGGACCCCTCTCTCCGATCCCAGGAAGACAATGTAATTCAAAGTATTGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAGTGCGTTAAGGGAGGTTGATACATCGAGACTATTATCAGAGATTCTTCAACACCCTCCTAAAGATGGTTATTCGAAGAAGATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAATTCAATGAACATCCTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGACTAGCATTTGACAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAAGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGTTGCACTGTCCTACTCAGTGCAAATAATGGATGAAGGAATTATACCACTCACTCCAAACGATGTTTTAGTCGATGCTCTTGTATCACCGTCTGGTGTGATTCCCATCAGCTCAGCTGGATTGGACAAAATAAAAGTATGA

Protein sequence

MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYKPLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIMDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV
Homology
BLAST of PI0014402 vs. ExPASy Swiss-Prot
Match: Q8L539 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=5FCL PE=1 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.2e-88
Identity = 157/228 (68.86%), Postives = 193/228 (84.65%), Query Frame = 0

Query: 101 SIKPENENQLKHIFDQKRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLC 160
           S   +N+ +L  IF QKRV+RS+VRK+LKAMDPSLR+Q+D  IQ  VLEAPWFKS + LC
Sbjct: 44  STTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLC 103

Query: 161 AYVSCSALREVDTSRLLSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANS 220
           AY+SC +L EVDTS++LSEILQHP  +   KK+YVP VEDKNS+MRM +IS M+DL+ANS
Sbjct: 104 AYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRMLHISHMEDLVANS 163

Query: 221 MNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQEL 280
           MNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRGGGYYDTFLK YQ+ 
Sbjct: 164 MNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDR 223

Query: 281 AKARNWKQPLLVALSYSVQIMDEGIIPLTPNDVLVDALVSPSGVIPIS 329
           AK + W+ PL+VALSYS QI+++G IP+TPNDVL+DALV+PSGV+PI+
Sbjct: 224 AKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPIT 270

BLAST of PI0014402 vs. ExPASy Swiss-Prot
Match: P49914 (5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 4.2e-19
Identity = 66/209 (31.58%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 117 KRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRL 176
           KR LR  +++ L+AM    R ++  V+   V+    ++ S+R+  ++S     E++T  +
Sbjct: 10  KRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQ--DEIETEEI 69

Query: 177 LSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAPVDGDG 236
           + +I Q        K  ++PR   +++HM M  I   ++   L   S NI +P    G+G
Sbjct: 70  IKDIFQR------GKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQP----GEG 129

Query: 237 NEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLK---NYQELAKARNWKQPL 296
           + RE+ + T   +DL  +PGL FDK G RLGRG GYYD +LK    +QE+       +P 
Sbjct: 130 DVREEALST-GGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEV-------KPY 189

Query: 297 LVALSYSVQIMDEGIIPLTPNDVLVDALV 320
            +AL++  QI  +  +P+  ND+ VD ++
Sbjct: 190 TLALAFKEQICLQ--VPVNENDMKVDEVL 196

BLAST of PI0014402 vs. ExPASy Swiss-Prot
Match: Q9D110 (5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 2.7e-18
Identity = 65/209 (31.10%), Postives = 117/209 (55.98%), Query Frame = 0

Query: 117 KRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRL 176
           KR LR+ +++ L+A+    R ++  ++   V+    +++S+R+  ++S     EV+T  +
Sbjct: 10  KRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHNQYQNSKRISIFLSMQ--DEVETEVI 69

Query: 177 LSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAPVDGDG 236
           + +I +        K  ++PR + +++HM M  ++  ++   L   S NI +P    G+G
Sbjct: 70  IKDIFKQ------GKICFIPRYQFQSNHMDMVRLTSSEEIALLPKTSWNIHQP----GEG 129

Query: 237 NEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLK---NYQELAKARNWKQPL 296
           + RE+ + T   +DL  LPGL FDK G RLGRG GYYDT+LK    +QE+       +P 
Sbjct: 130 DVREEALST-GGLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEV-------KPY 189

Query: 297 LVALSYSVQIMDEGIIPLTPNDVLVDALV 320
            +AL++  QI  +  IP+  +D+ VD ++
Sbjct: 190 TMALAFKEQICPQ--IPVDEHDMKVDEVL 196

BLAST of PI0014402 vs. ExPASy Swiss-Prot
Match: P80405 (5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.3e-17
Identity = 60/206 (29.13%), Postives = 112/206 (54.37%), Query Frame = 0

Query: 117 KRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRL 176
           KR LR+ +++ L+A+    R +   ++   V+    ++ SQR+  ++S     E++T  +
Sbjct: 10  KRSLRAELKQRLRAISAEERLRCQRLLTQKVIAHRQYQKSQRISIFLSMP--DEIETEEI 69

Query: 177 LSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIA---NSMNILEPAPVDGDG 236
           + +I Q        K  ++PR   +++HM M  ++  D++ +    S NI +P+    + 
Sbjct: 70  IKDIFQQ------GKVCFIPRYRLQSNHMDMVKLASADEISSLPKTSWNIHQPS----ES 129

Query: 237 NEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVA 296
           + RE+ + T   +DL  +PGL FD++G RLGRG GYYDT+L+   +   A+    P  +A
Sbjct: 130 DTREEALAT-GGLDLIFMPGLGFDRNGNRLGRGRGYYDTYLQRCLQQQGAK----PYTIA 189

Query: 297 LSYSVQIMDEGIIPLTPNDVLVDALV 320
           L++  QI  +  +P+   DV VD ++
Sbjct: 190 LAFREQICPQ--VPVDDTDVSVDEVL 196

BLAST of PI0014402 vs. ExPASy Swiss-Prot
Match: Q9P7W2 (Probable 5-formyltetrahydrofolate cyclo-ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC1703.08c PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.5e-13
Identity = 63/214 (29.44%), Postives = 105/214 (49.07%), Query Frame = 0

Query: 116 QKRVLRSSVRKAL-KAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTS 175
           +K  LR+ +  +L K  D  + SQ  ++ + +V E P +K  + +C Y++    +EV T 
Sbjct: 4   KKNQLRAILNSSLGKLADHIIDSQSISICKQVV-ELPEWKRCKNVCLYMNMPK-KEVRTR 63

Query: 176 RLLSEILQHPPKDGYSKKIYVPRVEDKNSH-MRMFNI-SRMDDLIANSMNILEPAPVDGD 235
            L+        K+G  K +++P+     SH M M+ +  + + L  N   I EP      
Sbjct: 64  CLIDVAF----KEG--KNVFIPKC--IGSHVMEMYQVFEKTESLTINKWGIAEP------ 123

Query: 236 GNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLL- 295
             E   +M  +   +L ++PG+AFD+   RLG G GYYD ++  YQ  A  +  +  +  
Sbjct: 124 NGESRKIMDDETDCELIIVPGVAFDEKLSRLGHGKGYYDNYISKYQSWALQKESRANMFK 183

Query: 296 VALSYSVQIMDEGIIPLTPNDVLVDALVSPSGVI 326
           V +    QI+    IP+   D  +DALV+P  VI
Sbjct: 184 VGICLKEQILPNREIPMDTRDQKLDALVTPEKVI 201

BLAST of PI0014402 vs. ExPASy TrEMBL
Match: A0A0A0LRK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.9e-185
Identity = 331/337 (98.22%), Postives = 335/337 (99.41%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           MVKYQLLQGNRTNLPVNGFQNSTTG RLTNQLSPAGVHLGRSPKP+KWAISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENE+QLKHIFDQKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPS RSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

BLAST of PI0014402 vs. ExPASy TrEMBL
Match: A0A1S3BVI2 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493953 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.8e-181
Identity = 326/337 (96.74%), Postives = 330/337 (97.92%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           MVKYQLLQGNRTNLPVNGFQNST GSRLTNQLSPAGVHLGRSPK RKWAISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTIGSRLTNQLSPAGVHLGRSPKHRKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSG PKMATNESIKPENE+QLKHIFDQKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGCPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKS+QRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSTQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVD DGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDSDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKA+NWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKAQNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTP DVLVDALVSPSGVIPISSA LD +KV
Sbjct: 301 MDEGIIPLTPKDVLVDALVSPSGVIPISSAALDIMKV 337

BLAST of PI0014402 vs. ExPASy TrEMBL
Match: A0A6J1JM66 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.9e-163
Identity = 298/337 (88.43%), Postives = 314/337 (93.18%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           M+K QLLQGN+TNLPV+G QNSTT SRLTN  SPAGVH+GRSPK R+ AISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
            LLPIAA+SLFN +  SAFQ +L PLSR SSG PKMA NE IKPENE QLK +F+QKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDN IQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVD LVSPSGVIPISSAGLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

BLAST of PI0014402 vs. ExPASy TrEMBL
Match: A0A6J1JCX7 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.3e-161
Identity = 297/339 (87.61%), Postives = 313/339 (92.33%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           M+K QLLQGN+TNLPV+G QNSTT SRLTN  SPAGVH+GRSPK R+ AISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
            LLPIAA+SLFN +  SAFQ +L PLSR SSG PKMA NE IKPENE QLK +F+QKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDN IQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED-- 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED  
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGN 240

Query: 241 VMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSV 300
            MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSV
Sbjct: 241 FMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300

Query: 301 QIMDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           QIMDEGIIPLTPNDVLVD LVSPSGVIPISSAGLDK+KV
Sbjct: 301 QIMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

BLAST of PI0014402 vs. ExPASy TrEMBL
Match: A0A6J1E0S0 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429770 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.3e-160
Identity = 292/337 (86.65%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           M+K QLLQGN+TNLPV+G QNSTT SRLTN  +PAGVH+GRSPK R+ A+SE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRNPAGVHVGRSPKARRRAVSESPMVRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
            LLPIAA+S FN +  +AFQ +L PLSR SS  PKMA NE IKPENE QLK +F+QKRV+
Sbjct: 61  -LLPIAASSPFNRLPPTAFQPALRPLSRCSSRSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDN IQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
            TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 LTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVD LVSPSGVIPISSAGLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

BLAST of PI0014402 vs. NCBI nr
Match: XP_004138292.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 8.0e-185
Identity = 331/337 (98.22%), Postives = 335/337 (99.41%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           MVKYQLLQGNRTNLPVNGFQNSTTG RLTNQLSPAGVHLGRSPKP+KWAISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENE+QLKHIFDQKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPS RSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

BLAST of PI0014402 vs. NCBI nr
Match: KGN63636.2 (hypothetical protein Csa_013278 [Cucumis sativus])

HSP 1 Score: 653.7 bits (1685), Expect = 8.8e-184
Identity = 329/337 (97.63%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           MVKYQLLQGNRTNLPVNGFQNSTTG RLTNQLSPAGVHLGRSPKP+KWAISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENE+QLKHIFDQKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPS RSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK  +
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKYAI 337

BLAST of PI0014402 vs. NCBI nr
Match: XP_008453155.1 (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 326/337 (96.74%), Postives = 330/337 (97.92%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           MVKYQLLQGNRTNLPVNGFQNST GSRLTNQLSPAGVHLGRSPK RKWAISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTIGSRLTNQLSPAGVHLGRSPKHRKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSG PKMATNESIKPENE+QLKHIFDQKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGCPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKS+QRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSTQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVD DGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDSDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKA+NWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKAQNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTP DVLVDALVSPSGVIPISSA LD +KV
Sbjct: 301 MDEGIIPLTPKDVLVDALVSPSGVIPISSAALDIMKV 337

BLAST of PI0014402 vs. NCBI nr
Match: XP_038879322.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Benincasa hispida])

HSP 1 Score: 600.5 bits (1547), Expect = 8.9e-168
Identity = 302/337 (89.61%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S T S+LTNQ SPAGVHLGRSPKPRKWAI EAPMLRNYK
Sbjct: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENE+QLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLR QEDN IQSIVLEAPWFKSSQRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVL+DALVSPSGVIPISS GLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDKMKV 337

BLAST of PI0014402 vs. NCBI nr
Match: XP_022988398.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 585.1 bits (1507), Expect = 3.8e-163
Identity = 298/337 (88.43%), Postives = 314/337 (93.18%), Query Frame = 0

Query: 1   MVKYQLLQGNRTNLPVNGFQNSTTGSRLTNQLSPAGVHLGRSPKPRKWAISEAPMLRNYK 60
           M+K QLLQGN+TNLPV+G QNSTT SRLTN  SPAGVH+GRSPK R+ AISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENENQLKHIFDQKRVL 120
            LLPIAA+SLFN +  SAFQ +L PLSR SSG PKMA NE IKPENE QLK +F+QKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKALKAMDPSLRSQEDN IQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKIKV 338
           MDEGIIPLTPNDVLVD LVSPSGVIPISSAGLDK+KV
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

BLAST of PI0014402 vs. TAIR 10
Match: AT5G13050.1 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 326.6 bits (836), Expect = 2.3e-89
Identity = 157/228 (68.86%), Postives = 193/228 (84.65%), Query Frame = 0

Query: 101 SIKPENENQLKHIFDQKRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLC 160
           S   +N+ +L  IF QKRV+RS+VRK+LKAMDPSLR+Q+D  IQ  VLEAPWFKS + LC
Sbjct: 44  STTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLC 103

Query: 161 AYVSCSALREVDTSRLLSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANS 220
           AY+SC +L EVDTS++LSEILQHP  +   KK+YVP VEDKNS+MRM +IS M+DL+ANS
Sbjct: 104 AYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRMLHISHMEDLVANS 163

Query: 221 MNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQEL 280
           MNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRGGGYYDTFLK YQ+ 
Sbjct: 164 MNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGGGYYDTFLKRYQDR 223

Query: 281 AKARNWKQPLLVALSYSVQIMDEGIIPLTPNDVLVDALVSPSGVIPIS 329
           AK + W+ PL+VALSYS QI+++G IP+TPNDVL+DALV+PSGV+PI+
Sbjct: 224 AKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVPIT 270

BLAST of PI0014402 vs. TAIR 10
Match: AT5G13050.2 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 233.8 bits (595), Expect = 2.0e-61
Identity = 114/166 (68.67%), Postives = 138/166 (83.13%), Query Frame = 0

Query: 101 SIKPENENQLKHIFDQKRVLRSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSSQRLC 160
           S   +N+ +L  IF QKRV+RS+VRK+LKAMDPSLR+Q+D  IQ  VLEAPWFKS + LC
Sbjct: 44  STTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLC 103

Query: 161 AYVSCSALREVDTSRLLSEILQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANS 220
           AY+SC +L EVDTS++LSEILQHP  +   KK+YVP VEDKNS+MRM +IS M+DL+ANS
Sbjct: 104 AYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRMLHISHMEDLVANS 163

Query: 221 MNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRG 267
           MNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRG
Sbjct: 164 MNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRG 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L5393.2e-8868.865-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
P499144.2e-1931.585-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2[more]
Q9D1102.7e-1831.105-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=... [more]
P804051.3e-1729.135-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS ... [more]
Q9P7W21.5e-1329.44Probable 5-formyltetrahydrofolate cyclo-ligase OS=Schizosaccharomyces pombe (str... [more]
Match NameE-valueIdentityDescription
A0A0A0LRK43.9e-18598.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1[more]
A0A1S3BVI29.8e-18196.745-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Cucumis melo ... [more]
A0A6J1JM661.9e-16388.435-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit... [more]
A0A6J1JCX72.3e-16187.615-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbit... [more]
A0A6J1E0S04.3e-16086.655-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
XP_004138292.18.0e-18598.225-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus][more]
KGN63636.28.8e-18497.63hypothetical protein Csa_013278 [Cucumis sativus][more]
XP_008453155.12.0e-18096.74PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Cucu... [more]
XP_038879322.18.9e-16889.615-formyltetrahydrofolate cyclo-ligase, mitochondrial [Benincasa hispida][more]
XP_022988398.13.8e-16388.435-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT5G13050.12.3e-8968.865-formyltetrahydrofolate cycloligase [more]
AT5G13050.22.0e-6168.675-formyltetrahydrofolate cycloligase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0241855-formyltetrahydrofolate cyclo-ligase-like domain superfamilyGENE3D3.40.50.10420coord: 109..324
e-value: 9.6E-54
score: 183.9
IPR0026985-formyltetrahydrofolate cyclo-ligasePFAMPF018125-FTHF_cyc-ligcoord: 117..321
e-value: 4.4E-32
score: 111.5
IPR0026985-formyltetrahydrofolate cyclo-ligaseTIGRFAMTIGR02727TIGR02727coord: 117..320
e-value: 2.2E-29
score: 100.6
NoneNo IPR availablePIRSRPIRSR006806-1PIRSR006806-1coord: 116..230
e-value: 8.3E-11
score: 40.0
coord: 244..320
e-value: 5.0E-17
score: 60.2
NoneNo IPR availablePANTHERPTHR23407:SF105-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE, MITOCHONDRIAL-LIKE ISOFORM X1coord: 90..334
NoneNo IPR availablePANTHERPTHR23407ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASEcoord: 90..334
IPR037171NagB/RpiA transferase-likeSUPERFAMILY100950NagB/RpiA/CoA transferase-likecoord: 116..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0014402.1PI0014402.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0009396 folic acid-containing compound biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005739 mitochondrion
cellular_component GO:0031213 RSF complex
molecular_function GO:0030272 5-formyltetrahydrofolate cyclo-ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding