PI0014386 (gene) Melon (PI 482460) v1

Overview
NamePI0014386
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr07: 4626538 .. 4629620 (-)
RNA-Seq ExpressionPI0014386
SyntenyPI0014386
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCCTCGTCCGACGGCATTTTGCCGTCGCTTAAAGTTAAGACAGAACTTTATAACCCTACTGCTAACTGGGCTTTTGTCCCTTTGAGGAGAGCATTTCAATACAGTAAGATTCATTGCTTACTTTTTTTTTTTTACCCCCTTTTCTCTGTGTTTCTATTTGCGAACATTGGATACTGAATTGATTGTGTTGGGCTTTCTACTTGGGAGGAAGCAAATGCGTAATGAATAGGAGAAGTTTTGCTTTTAGGGGAATTGTGGCTTCTGGAGTTTCTGTTATGGGCTCTTCTGCAGTTATACAGCCTGCTCAAGGTCAGAGTTTGTATATATATGTATTTGGTTTAGTGGATCTTGCTTGTAGATTATTTGAAACGGGCGTCCTTGATGTTGGCTTATTAAATGCAACTTGGCTAAGGTTTTGAATTATTCTTTTTGTAGGGATTGAAAGATTACCATTCAAACCAGAAGGTTATAACTTCTGGACATGGCGGGGTCACAAGATACATTATGTTGTACAAGGAGAAGGTCCTCCGATAGTTCTTATCCATGGTTTTGGTGCTTCTGTATTTCATTGGAGGTGTGATTTAGTTGCCTTTTTTGGGTTTGCTTTTACTTCTAAATCGTTATTTTTTTTTTATGAATGAGTTGGAAAAATATTAAGCTCAAGTTTTTGGTATTAGATCTTCAGTTCAAATTCTGTATCTTTTTCTTCCTTTTTCGATTTAATATCAATAATCTAGGTATTGGAACTGTGGATTACAATAATTATTTGAGTTCATGTGTAATCACTGTGTTAAAAAATGGATTAAATCAAAGAACTTGAACGTCTGTGTCTTGTGGCAACCGACTGCATCTTTGTTTTCTTTATCAATTGAGTGATTCCATGACATTTTATGTTTTTTCCCCTTGAAGGTATAACATACCAGAGCTGGCTAAAAAGTACAAGGTCTATGCCTTAGACTTGCTGGGATTTGGGTGGAGCGATAAAGCAATTATTGAGTACGATGCCATGGTCTGGAGGGACCAAGTTGTCGATTTTATGAAGGAAATAGTGAAACAGCCTGCAGTTTTAGTTGGGAACAGGTAATGATACGGACTAAATATAAGTTTAAATGGATTATGGGATGTTTTAAGTGTTCTTGTTTGGACTTTGCTTTATAAAATGATGTGTACTCTAAATTATTATCATCACAATTTGATGCTTATGTATAACTATTTAGCGTTTCAGTTGTCATTTGTTAGTGAAGTATGATTTGATTGTTGCTCGTTCACATTCACATTTCCTTCTTTTATCAAACTAAGCTCTTTTTCTTGAGAAAATTTTTTTAGCGGTAAAATAGCTTTCTACTTCCTTTTTCAAACCACTTTTAGGAAGTTTTTTTTTTGGGTGGCTTAATAGTTTATGTTTGTTGCAGAAAATGCCACTTTCAAATACACAATTTCATCACAAATAACTTCATTTTGTTCTAAAAAATAGGTCATTCATAAAAGCAAACTAAAAAAGTATAATTTATATGCCTACGATTTTGTTTAGTTGTCATTTCAATGTCTAAAGACGTGCATGTTTCAAAGTATGGTACCTATTTTTTGGATAAAAAGTATATATGTCTAAGTTGCAGGCACTCCCTACTTCAACTTAGATCTCAACTCGACTTTCTTGGTCTAATTAATCGGCCTCATTTCGCGAGTTTTAGCCTATTCTAACAATATTGTTTCCTCATATTTCAGCATTTTAGCCTATTCTATAACCATGGTTAAAATTAGCTTCCTTTCTTCATTATGGATTTTTTATACTTTTTGATCTACGGTTGCAATGGCAAAACAAAAGGTAAAAGTTTGTCCACGTTGGTTGTCAATAATTGTGCCTGGAATGTGACGATGCTTTTAATGAAACTAATTTTGGATTATGCAATTGAAGTGACTGGTTCATGGTTCTTTGGCAGCCTTGGAGGGTTTACTGCCTTGGTTTCTGCAGCAGGGTTGCCGGAGCAAGTTCGTGGAGTTGTATTACTAAACTCCGCTGGGCAGTTTGGAGATGTAAATAAGTTGACAGAGGAGCCAGAAGAAACCAGCTTCCAGAAGTTCATTACGAAGCCACTGAAGGATTTTTTTCAGCGAATCTTTCTTGGAGTTCTTTTCTGGCAAACCAAGCAACCCGCTAGAATTGTTTCTGTTTTGAAGAGTGTAAGTGGGTGGCTACGCTCTTTGTTTTAAGGCATTTCTAGACTCTTGGAGATCATGTTTGGTATTCCTAAATAATAACTTGTAATTCGTAAAGATGAAAACCAACATTATACCAAAAATTTTGAATTTTTTGTCTCAAATGATGATACACTTTTCATAAGCATTTAAGGTTGGCTTCCTGGTGTATGTTATGCACACTTGAACTTCTTTGATTCATCATGGTTGCTCTTACAGGTATACATAAATTCTGAAAATGTGGATGATTATCTGGTAGAGTCTATATCGATGCCAGCAGCTGATCCAAATGCTAGAGAAGTTTACTACAGGTACGTATTTTTACTTGTCAGAGTTTTTCATGACAAATCTCTCCCTTGTACTGCTCACCTGGATGCAGCTCGTCTTCTTGCTTCATCTTGGTGTTTTCTCAGGATATTAGTTTGAATTGACATCATGTTTAATCCTTTGACTCATTTCATTATATCAATGAAAGTACTAGGAATATATGTTAAAGATTTGCGTACTCTTTATCTATCAATTTAAGCTCTTGAAATTAGTGGTAGTCTAAAACATGGATCTTAGATATTGCTTTTAATTCCCTGGAACATGAAGTTTGTTGATTTTGGTGCAGATTGATGACAAGATTCATGTTCAATCAAAGCAAATATACTCTAAACAGTGTATTGAGTGAGCTGCGTTGTCCACTGTTATTGTTGTGGGGTGATTTAGATCCATGGGTTGGTCCTGCCAAGGCCAACAGAATCAAAGAATTTTATCCCAACACAACTCTTGTAAATTTGAAAGCAGGGCATTGCCCACATGATGAGGTTCCTGAGCTTGTCAATTCTGCTCTGATGGATTGGCTAGCAACTCTACAACAACCTCAACCAACACTCTGA

mRNA sequence

ATGATTTCCTCGTCCGACGGCATTTTGCCGTCGCTTAAAGTTAAGACAGAACTTTATAACCCTACTGCTAACTGGGCTTTTGTCCCTTTGAGGAGAGCATTTCAATACAGAAGCAAATGCGTAATGAATAGGAGAAGTTTTGCTTTTAGGGGAATTGTGGCTTCTGGAGTTTCTGTTATGGGCTCTTCTGCAGTTATACAGCCTGCTCAAGGGATTGAAAGATTACCATTCAAACCAGAAGGTTATAACTTCTGGACATGGCGGGGTCACAAGATACATTATGTTGTACAAGGAGAAGGTCCTCCGATAGTTCTTATCCATGGTTTTGGTGCTTCTGTATTTCATTGGAGGTATAACATACCAGAGCTGGCTAAAAAGTACAAGGTCTATGCCTTAGACTTGCTGGGATTTGGGTGGAGCGATAAAGCAATTATTGAGTACGATGCCATGGTCTGGAGGGACCAAGTTGTCGATTTTATGAAGGAAATAGTGAAACAGCCTGCAGTTTTAGTTGGGAACAGCCTTGGAGGGTTTACTGCCTTGGTTTCTGCAGCAGGGTTGCCGGAGCAAGTTCGTGGAGTTGTATTACTAAACTCCGCTGGGCAGTTTGGAGATGTAAATAAGTTGACAGAGGAGCCAGAAGAAACCAGCTTCCAGAAGTTCATTACGAAGCCACTGAAGGATTTTTTTCAGCGAATCTTTCTTGGAGTTCTTTTCTGGCAAACCAAGCAACCCGCTAGAATTGTTTCTGTTTTGAAGAGTGTATACATAAATTCTGAAAATGTGGATGATTATCTGGTAGAGTCTATATCGATGCCAGCAGCTGATCCAAATGCTAGAGAAGTTTACTACAGATTGATGACAAGATTCATGTTCAATCAAAGCAAATATACTCTAAACAGTGTATTGAGTGAGCTGCGTTGTCCACTGTTATTGTTGTGGGGTGATTTAGATCCATGGGTTGGTCCTGCCAAGGCCAACAGAATCAAAGAATTTTATCCCAACACAACTCTTGTAAATTTGAAAGCAGGGCATTGCCCACATGATGAGGTTCCTGAGCTTGTCAATTCTGCTCTGATGGATTGGCTAGCAACTCTACAACAACCTCAACCAACACTCTGA

Coding sequence (CDS)

ATGATTTCCTCGTCCGACGGCATTTTGCCGTCGCTTAAAGTTAAGACAGAACTTTATAACCCTACTGCTAACTGGGCTTTTGTCCCTTTGAGGAGAGCATTTCAATACAGAAGCAAATGCGTAATGAATAGGAGAAGTTTTGCTTTTAGGGGAATTGTGGCTTCTGGAGTTTCTGTTATGGGCTCTTCTGCAGTTATACAGCCTGCTCAAGGGATTGAAAGATTACCATTCAAACCAGAAGGTTATAACTTCTGGACATGGCGGGGTCACAAGATACATTATGTTGTACAAGGAGAAGGTCCTCCGATAGTTCTTATCCATGGTTTTGGTGCTTCTGTATTTCATTGGAGGTATAACATACCAGAGCTGGCTAAAAAGTACAAGGTCTATGCCTTAGACTTGCTGGGATTTGGGTGGAGCGATAAAGCAATTATTGAGTACGATGCCATGGTCTGGAGGGACCAAGTTGTCGATTTTATGAAGGAAATAGTGAAACAGCCTGCAGTTTTAGTTGGGAACAGCCTTGGAGGGTTTACTGCCTTGGTTTCTGCAGCAGGGTTGCCGGAGCAAGTTCGTGGAGTTGTATTACTAAACTCCGCTGGGCAGTTTGGAGATGTAAATAAGTTGACAGAGGAGCCAGAAGAAACCAGCTTCCAGAAGTTCATTACGAAGCCACTGAAGGATTTTTTTCAGCGAATCTTTCTTGGAGTTCTTTTCTGGCAAACCAAGCAACCCGCTAGAATTGTTTCTGTTTTGAAGAGTGTATACATAAATTCTGAAAATGTGGATGATTATCTGGTAGAGTCTATATCGATGCCAGCAGCTGATCCAAATGCTAGAGAAGTTTACTACAGATTGATGACAAGATTCATGTTCAATCAAAGCAAATATACTCTAAACAGTGTATTGAGTGAGCTGCGTTGTCCACTGTTATTGTTGTGGGGTGATTTAGATCCATGGGTTGGTCCTGCCAAGGCCAACAGAATCAAAGAATTTTATCCCAACACAACTCTTGTAAATTTGAAAGCAGGGCATTGCCCACATGATGAGGTTCCTGAGCTTGTCAATTCTGCTCTGATGGATTGGCTAGCAACTCTACAACAACCTCAACCAACACTCTGA

Protein sequence

MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVMGSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFWQTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLNSVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALMDWLATLQQPQPTL
Homology
BLAST of PI0014386 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.6e-33
Identity = 97/321 (30.22%), Postives = 146/321 (45.48%), Query Frame = 0

Query: 91  KIHYVVQG----EGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG-------- 150
           ++HY   G    + P ++ + GFG   FH+   + +L + Y+V+A+D LG G        
Sbjct: 103 RVHYEKAGCDNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDP 162

Query: 151 -------------------------WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGN 210
                                    W+D+ +   D  +WRDQV  F++E++ +P  + GN
Sbjct: 163 TTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLD--LWRDQVQYFVEEVIGEPVYIAGN 222

Query: 211 SLGGFTALVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPE-ETSFQKFITKPLKDFFQR 270
           SLGG+ AL  AA  P  V+GV LLN+   +G        P+    F      PL +  ++
Sbjct: 223 SLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKK 282

Query: 271 IFLGVLFWQ-TKQPARIVSVLKSVY----INSENVDDYLVESISMPAADPNAREVYYRLM 330
           I    L WQ    P  I  +LK VY    IN + V   +VE    PAA  +   +     
Sbjct: 283 I--TELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPG 342

Query: 331 TRFMFNQSKYTLNSVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLK-AGHC 368
               F+++     S   E    + L++G  DPWV P    +IK+  PN     +  AGHC
Sbjct: 343 GELSFSEAL----SRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHC 402

BLAST of PI0014386 vs. ExPASy Swiss-Prot
Match: P19076 (2-hydroxymuconate semialdehyde hydrolase OS=Pseudomonas sp. (strain CF600) OX=79676 GN=dmpD PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 49/108 (45.37%), Postives = 67/108 (62.04%), Query Frame = 0

Query: 98  GEGPPIVLIHGFGASV---FHWRYNIPELAKKYKVYALDLLGFGWSDK-AIIEYDAMVWR 157
           G G P+++IHG G  V    +WR  +PELAK  +V A D+LGFG+S++ A  +Y+  VW 
Sbjct: 27  GAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPADAQYNRDVWV 86

Query: 158 DQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVLLNSAG 202
           D  V  +  +  + A LVGNS GG  AL  A   PE+VR +VL+ SAG
Sbjct: 87  DHAVGVLDALEIEQADLVGNSFGGGIALALAIRHPERVRRLVLMGSAG 134

BLAST of PI0014386 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-13
Identity = 43/129 (33.33%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 79  PEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 138
           P    F T  G++  + + G GP I+LIHG G +   W     +LA+++ V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 139 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVLLN 198
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 199 SAGQFGDVN 208
           + G   DVN
Sbjct: 136 AGGVTKDVN 144

BLAST of PI0014386 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-13
Identity = 43/129 (33.33%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 79  PEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 138
           P    F T  G++  + + G GP I+LIHG G +   W     +LA+++ V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 139 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVLLN 198
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 199 SAGQFGDVN 208
           + G   DVN
Sbjct: 136 AGGVTKDVN 144

BLAST of PI0014386 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-13
Identity = 43/129 (33.33%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 79  PEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 138
           P    F T  G++  + + G GP I+LIHG G +   W     +LA+++ V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 139 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVLLN 198
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 199 SAGQFGDVN 208
           + G   DVN
Sbjct: 136 AGGVTKDVN 144

BLAST of PI0014386 vs. ExPASy TrEMBL
Match: A0A5A7TNN5 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004850 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.6e-216
Identity = 369/373 (98.93%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSS+GI PSLKVKTELYNPTANW FVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVY+NSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQPQPTL
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of PI0014386 vs. ExPASy TrEMBL
Match: A0A1S3BSY0 (pheophytinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493182 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.6e-216
Identity = 369/373 (98.93%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSS+GI PSLKVKTELYNPTANW FVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVY+NSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQPQPTL
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of PI0014386 vs. ExPASy TrEMBL
Match: A0A0A0KWQ9 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046820 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 3.7e-213
Identity = 363/373 (97.32%), Postives = 369/373 (98.93%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSS+GI PSLKVKTELYNPTANW FVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQG+ERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDF KE+VKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFTKEVVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFI+KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFISKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS+PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISIPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQ QPTL
Sbjct: 361 DWLATLQQLQPTL 373

BLAST of PI0014386 vs. ExPASy TrEMBL
Match: A0A6J1FN92 (pheophytinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446991 PE=4 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.1e-201
Identity = 347/373 (93.03%), Postives = 357/373 (95.71%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MIS + GI PSL  KTE+YN  ANW FVPLRRAFQ+RS+CVMNRRSFAFRGIVASGVSV 
Sbjct: 1   MISFAGGISPSL--KTEIYNLPANWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVT 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGL EQVRGVVLLNSAGQFGD NKL +EPEETSF+KFI KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLAEQVRGVVLLNSAGQFGDANKLIDEPEETSFKKFILKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS PAADPNA EVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKE YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWL+TLQQPQP++
Sbjct: 361 DWLSTLQQPQPSV 371

BLAST of PI0014386 vs. ExPASy TrEMBL
Match: A0A6J1IZR3 (pheophytinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480611 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 4.7e-200
Identity = 344/373 (92.23%), Postives = 356/373 (95.44%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MIS + GI PSL  KTE+YNP A W FVPLRRAFQ+RS+CVMNRRSFAFRGIVASGVSV 
Sbjct: 1   MISFAGGISPSL--KTEIYNPPAYWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVT 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGL EQVRGVVLLNSAGQFGD NKL +EPEETSF+KFI KPLKDFFQRI LGV+FW
Sbjct: 181 LVSAAGLAEQVRGVVLLNSAGQFGDDNKLIDEPEETSFKKFILKPLKDFFQRIVLGVIFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS PAADPNA EVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKE YPNTTLVNLKAGHCPHDEVPELVNSAL+
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALV 360

Query: 361 DWLATLQQPQPTL 374
           DWL+TLQQPQP++
Sbjct: 361 DWLSTLQQPQPSV 371

BLAST of PI0014386 vs. NCBI nr
Match: XP_008452064.1 (PREDICTED: pheophytinase, chloroplastic isoform X1 [Cucumis melo] >KAA0044810.1 pheophytinase [Cucumis melo var. makuwa] >TYK16654.1 pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 761.5 bits (1965), Expect = 3.3e-216
Identity = 369/373 (98.93%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSS+GI PSLKVKTELYNPTANW FVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVY+NSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQPQPTL
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of PI0014386 vs. NCBI nr
Match: XP_038896087.1 (pheophytinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 750.7 bits (1937), Expect = 5.9e-213
Identity = 363/373 (97.32%), Postives = 370/373 (99.20%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSSDGI PSLKVKTELYNPTANWAFVPLRRAFQ+RSKC+MNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSDGISPSLKVKTELYNPTANWAFVPLRRAFQHRSKCLMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAV+QPAQGIERLPFKPEGYNFWTWRGHKIHYVV+GEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVLQPAQGIERLPFKPEGYNFWTWRGHKIHYVVKGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIV+QPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVEQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLP+QVRGVVLLNSAGQFGDVNKLT+EPEETSFQKFI KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPDQVRGVVLLNSAGQFGDVNKLTDEPEETSFQKFILKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSEL CPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELCCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQPQPTL
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of PI0014386 vs. NCBI nr
Match: XP_004146511.1 (pheophytinase, chloroplastic [Cucumis sativus] >KGN53319.1 hypothetical protein Csa_015132 [Cucumis sativus])

HSP 1 Score: 750.4 bits (1936), Expect = 7.7e-213
Identity = 363/373 (97.32%), Postives = 369/373 (98.93%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MISSS+GI PSLKVKTELYNPTANW FVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQG+ERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDF KE+VKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFTKEVVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFI+KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFISKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS+PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISIPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWLATLQQ QPTL
Sbjct: 361 DWLATLQQLQPTL 373

BLAST of PI0014386 vs. NCBI nr
Match: KAG6600575.1 (Pheophytinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 713.8 bits (1841), Expect = 7.9e-202
Identity = 347/373 (93.03%), Postives = 357/373 (95.71%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MIS + GI PSL  KTE+YNP ANW FVPLRRAFQ+RS+CVMNRRSFAFRGIVASGVSV 
Sbjct: 83  MISFAGGISPSL--KTEIYNPPANWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVT 142

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 143 GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 202

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 203 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 262

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGL EQVRGVVLLNSAGQFGD NKL +EPEETSF+K I KPLKDFFQRIFLGVLFW
Sbjct: 263 LVSAAGLAEQVRGVVLLNSAGQFGDANKLIDEPEETSFKKLILKPLKDFFQRIFLGVLFW 322

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS PAADPNA EVYYRLMTRFMFNQSKYTLN
Sbjct: 323 QTKQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLN 382

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKE YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 383 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALM 442

Query: 361 DWLATLQQPQPTL 374
           DWL+TLQQPQP++
Sbjct: 443 DWLSTLQQPQPSV 453

BLAST of PI0014386 vs. NCBI nr
Match: XP_022941712.1 (pheophytinase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 712.2 bits (1837), Expect = 2.3e-201
Identity = 347/373 (93.03%), Postives = 357/373 (95.71%), Query Frame = 0

Query: 1   MISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60
           MIS + GI PSL  KTE+YN  ANW FVPLRRAFQ+RS+CVMNRRSFAFRGIVASGVSV 
Sbjct: 1   MISFAGGISPSL--KTEIYNLPANWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVT 60

Query: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120
           GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAKKYKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240
           LVSAAGL EQVRGVVLLNSAGQFGD NKL +EPEETSF+KFI KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLAEQVRGVVLLNSAGQFGDANKLIDEPEETSFKKFILKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARIVSVLKSVYINSENVDDYLVESIS PAADPNA EVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVLSELRCPLLLLWGDLDPWVGPAKANRIKE YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLATLQQPQPTL 374
           DWL+TLQQPQP++
Sbjct: 361 DWLSTLQQPQPSV 371

BLAST of PI0014386 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 538.9 bits (1387), Expect = 3.3e-153
Identity = 264/365 (72.33%), Postives = 304/365 (83.29%), Query Frame = 0

Query: 2   ISSSDGILPSLKVKTELYNPTANWAFVPLRRAFQYRSKCVMNRRSFAFR-GIVASGVSVM 61
           +S+S  + PS  V+TEL++ +++   +   R  + R+KC ++RR FA R GIVASGVSVM
Sbjct: 1   MSASCALTPS--VRTELFSSSSSKRSIFPARICRPRNKCEISRRDFAIRGGIVASGVSVM 60

Query: 62  G-SSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYN 121
             SSA  Q  QG ERL FKPEGYNFW WRGHKIHYVVQGEG P+VLIHGFGASVFHWRYN
Sbjct: 61  DTSSASSQSVQGSERLAFKPEGYNFWEWRGHKIHYVVQGEGSPLVLIHGFGASVFHWRYN 120

Query: 122 IPELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFT 181
           IPELAKKYKVYALDLLGFGWSDKA+IEYDAMVW DQV+DFMKE+VK+PAV+VGNSLGGFT
Sbjct: 121 IPELAKKYKVYALDLLGFGWSDKALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFT 180

Query: 182 ALVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLF 241
           AL  A GLPEQV GV LLNSAGQF   ++  EE +ET   KFI KPLK+ FQR+ LG LF
Sbjct: 181 ALSVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLGFLF 240

Query: 242 WQTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTL 301
           WQ KQP+RI SVLKSVYI+S NVDDYLVESIS PA DPNA EVYYRLMTRF+ NQS+YTL
Sbjct: 241 WQAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTL 300

Query: 302 NSVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSAL 361
           +SVLS++ CPLLL+WGDLDPWVGPAKA +IK FY N++LV+L+AGHCPHDEVPE VN AL
Sbjct: 301 DSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHLQAGHCPHDEVPEAVNKAL 360

Query: 362 MDWLA 365
           +DWL+
Sbjct: 361 LDWLS 363

BLAST of PI0014386 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 506.1 bits (1302), Expect = 2.3e-143
Identity = 237/303 (78.22%), Postives = 263/303 (86.80%), Query Frame = 0

Query: 62  SSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIP 121
           SSA  Q  QG ERL FKPEGYNFW WRGHKIHYVVQGEG P+VLIHGFGASVFHWRYNIP
Sbjct: 4   SSASSQSVQGSERLAFKPEGYNFWEWRGHKIHYVVQGEGSPLVLIHGFGASVFHWRYNIP 63

Query: 122 ELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTAL 181
           ELAKKYKVYALDLLGFGWSDKA+IEYDAMVW DQV+DFMKE+VK+PAV+VGNSLGGFTAL
Sbjct: 64  ELAKKYKVYALDLLGFGWSDKALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTAL 123

Query: 182 VSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFWQ 241
             A GLPEQV GV LLNSAGQF   ++  EE +ET   KFI KPLK+ FQR+ LG LFWQ
Sbjct: 124 SVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLGFLFWQ 183

Query: 242 TKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLNS 301
            KQP+RI SVLKSVYI+S NVDDYLVESIS PA DPNA EVYYRLMTRF+ NQS+YTL+S
Sbjct: 184 AKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLDS 243

Query: 302 VLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALMD 361
           VLS++ CPLLL+WGDLDPWVGPAKA +IK FY N++LV+L+AGHCPHDEVPE VN AL+D
Sbjct: 244 VLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHLQAGHCPHDEVPEAVNKALLD 303

Query: 362 WLA 365
           WL+
Sbjct: 304 WLS 306

BLAST of PI0014386 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.8e-43
Identity = 103/299 (34.45%), Postives = 159/299 (53.18%), Query Frame = 0

Query: 85  WTWRGHKIHYVVQG-EGPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKA 144
           W W+G+ I Y   G  GP +VL+HGFGA+  HWR N P L K ++VY++DL+G+G+SDK 
Sbjct: 78  WKWKGYSIRYQCAGTSGPALVLVHGFGANSDHWRKNTPILGKTHRVYSIDLIGYGYSDKP 137

Query: 145 IIE-------YDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLPEQVRGVVL 204
                     Y    W +Q+ DF  ++VK  A  + NS+GG   L +A   PE  RG++L
Sbjct: 138 NPREFGGEPFYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLML 197

Query: 205 LNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIF----LGVLFWQT-KQPARIVSV 264
           +N + +   + K          Q FI +P    FQ +     +G LF+++  +P  + S+
Sbjct: 198 INISLRMLHIKK----------QPFIGRPFIKSFQNLLRNTPVGKLFFKSIAKPETVKSI 257

Query: 265 LKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLNSVLSELRCPLL 324
           L   Y +S  V D LVE+I  P  +P A +V+      F+          +L  ++CP+L
Sbjct: 258 LCQCYHDSSQVTDELVEAILRPGLEPGAVDVF----LEFICYSGGPLPEDLLPLVKCPVL 317

Query: 325 LLWGDLDPW----VGPAKAN--RIKEFYPNTTLVNLKAGHCPHDEVPELVNSALMDWLA 365
           + WG+ DPW    +G A +N   +++F     +V   AGHCP DE PE+VN  +  ++A
Sbjct: 318 IAWGEKDPWEPIELGRAYSNFDAVEDF-----VVLPDAGHCPQDEKPEMVNPLIESFVA 357

BLAST of PI0014386 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 4.4e-41
Identity = 115/343 (33.53%), Postives = 176/343 (51.31%), Query Frame = 0

Query: 42  MNRRSFAFRGIVASGVSVMGSSAVIQPAQGIERLPFKPEGYNFWTWRG-HKIHYVVQGE- 101
           + R     R   +S  +V G   V    + +E L    E    W W+G + ++Y V+   
Sbjct: 29  LRRDRVCVRATASSSATVSGGGVV----EAVE-LAEIGERSKKWKWKGEYSVNYFVKDSP 88

Query: 102 ------GPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKAI-IEYDAMVW 161
                    ++L+HGFGAS+ HWR NI  L+K + VYA+DLLGFG SDK     Y    W
Sbjct: 89  EEVTPASQTVLLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESW 148

Query: 162 RDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAA-GLPEQVRGVVLLNSAGQFGDVNKLTE 221
            + +++F++E+V++P +L+GNS+G    +++A+    + V+G+VLLN AG  G  NK   
Sbjct: 149 AELILNFLEEVVQKPTILIGNSVGSLACVIAASESRGDLVKGLVLLNCAG--GMNNKAVF 208

Query: 222 EPEETSFQKFITKPLKDFF--QRIFLGVLFWQTKQPARIVSVLKSVYINSENVDDYLVES 281
           +         +   L DF   QR     LF + K    + ++L +VY N +NVDD LVE 
Sbjct: 209 DDWRIKLLMPLLL-LIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEI 268

Query: 282 ISMPAADPNAREVYYRLMTRFMFNQSKYTLNSVLSELRCPLLLLWGDLD---PWVGPAKA 341
           I+ PA    A + +  ++T             ++ E+  P+L+LWGD D   P  GP   
Sbjct: 269 IAGPANTEGALDAFVSILT----GPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGK 328

Query: 342 --NRIKEFYPNTTLVNLK-AGHCPHDEVPELVNSALMDWLATL 367
               + +  PN  L  L+  GHCP D+ P+LV+  L+ WLA L
Sbjct: 329 YFTSLPDQLPNFNLYVLQGVGHCPQDDRPDLVHERLLPWLAQL 359

BLAST of PI0014386 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-40
Identity = 116/355 (32.68%), Postives = 177/355 (49.86%), Query Frame = 0

Query: 42  MNRRSFAFRGIVASGVSVMGSSAVIQPAQGIERLPFKPEGYNFWTWRG-HKIHYVVQGE- 101
           + R     R   +S  +V G   V    + +E L    E    W W+G + ++Y V+   
Sbjct: 29  LRRDRVCVRATASSSATVSGGGVV----EAVE-LAEIGERSKKWKWKGEYSVNYFVKDSP 88

Query: 102 ------GPPIVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKAI-IEYDAMVW 161
                    ++L+HGFGAS+ HWR NI  L+K + VYA+DLLGFG SDK     Y    W
Sbjct: 89  EEVTPASQTVLLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESW 148

Query: 162 RDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAAGLP-------------EQVRGVVLLNS 221
            + +++F++E+V++P +L+GNS+G    +++A+G               + V+G+VLLN 
Sbjct: 149 AELILNFLEEVVQKPTILIGNSVGSLACVIAASGTKFLIYLEKKTESRGDLVKGLVLLNC 208

Query: 222 AGQFGDVNKLTEEPEETSFQKFITKPLKDFF--QRIFLGVLFWQTKQPARIVSVLKSVYI 281
           AG  G  NK   +         +   L DF   QR     LF + K    + ++L +VY 
Sbjct: 209 AG--GMNNKAVFDDWRIKLLMPLLL-LIDFLLKQRGIASALFNRVKDRENLKNILTNVYG 268

Query: 282 NSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLNSVLSELRCPLLLLWGDL 341
           N +NVDD LVE I+ PA    A + +  ++T             ++ E+  P+L+LWGD 
Sbjct: 269 NKDNVDDTLVEIIAGPANTEGALDAFVSILT----GPPGPNPIKLIPEITKPVLVLWGDQ 328

Query: 342 D---PWVGPAKA--NRIKEFYPNTTLVNLK-AGHCPHDEVPELVNSALMDWLATL 367
           D   P  GP       + +  PN  L  L+  GHCP D+ P+LV+  L+ WLA L
Sbjct: 329 DGLTPLDGPVGKYFTSLPDQLPNFNLYVLQGVGHCPQDDRPDLVHERLLPWLAQL 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFZ15.6e-3330.22Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1[more]
P190761.1e-1545.372-hydroxymuconate semialdehyde hydrolase OS=Pseudomonas sp. (strain CF600) OX=79... [more]
P9WNH21.3e-1333.33Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
P9WNH31.3e-1333.33Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ... [more]
P0A5731.3e-1333.33Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2... [more]
Match NameE-valueIdentityDescription
A0A5A7TNN51.6e-21698.93Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004850 ... [more]
A0A1S3BSY01.6e-21698.93pheophytinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493182 ... [more]
A0A0A0KWQ93.7e-21397.32AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046... [more]
A0A6J1FN921.1e-20193.03pheophytinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446991 ... [more]
A0A6J1IZR34.7e-20092.23pheophytinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480611 PE... [more]
Match NameE-valueIdentityDescription
XP_008452064.13.3e-21698.93PREDICTED: pheophytinase, chloroplastic isoform X1 [Cucumis melo] >KAA0044810.1 ... [more]
XP_038896087.15.9e-21397.32pheophytinase, chloroplastic [Benincasa hispida][more]
XP_004146511.17.7e-21397.32pheophytinase, chloroplastic [Cucumis sativus] >KGN53319.1 hypothetical protein ... [more]
KAG6600575.17.9e-20293.03Pheophytinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022941712.12.3e-20193.03pheophytinase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G36530.23.3e-15372.33alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.12.3e-14378.22alpha/beta-Hydrolases superfamily protein [more]
AT5G19850.12.8e-4334.45alpha/beta-Hydrolases superfamily protein [more]
AT5G38520.14.4e-4133.53alpha/beta-Hydrolases superfamily protein [more]
AT5G38520.21.3e-4032.68alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 170..183
score: 53.2
coord: 184..197
score: 33.25
coord: 126..141
score: 45.04
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 103..357
e-value: 3.8E-21
score: 76.7
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 340..362
score: 23.72
coord: 184..197
score: 32.79
coord: 107..125
score: 40.43
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 81..367
e-value: 4.4E-60
score: 205.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 83..363
NoneNo IPR availablePANTHERPTHR46438ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 14..367
NoneNo IPR availablePANTHERPTHR46438:SF8PHEOPHYTINASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 14..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0014386.1PI0014386.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003824 catalytic activity