PI0014314 (gene) Melon (PI 482460) v1

Overview


Name	PI0014314
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr04: 11285522 .. 11286795 (-)
RNA-Seq Expression	PI0014314
Synteny	PI0014314

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTTCACTTTCATTCCCATAGAGATAAACAAAACTAGAGAGAAAAAAAAAATGGAGGGAGAAATCATAAACTTAACCAAGGTATGGTTAACTGTCTGTATCTCTCTCTCTTACTGCCACGCCGCCGGAAAACTCCTCAATCCCGGTGCTGGCCGCTTCCTCGCCATCGCCCCCGTCGTCCTCCTCTTCTTCCTCCTTCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTTGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCACTGCCGGAGATGGTCCTCTCTCCACTACCCCGCCGCTTCCTCTCCGCCGATTCATAGCCATATCTTGCTTGCCGATCAAGATCCTGGAAAACCCATCGGAGAAATCGGCCCCCAATAATCGAGCGGAAACCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAGAGCTCTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTTCTCGGAATCGAGCTGGAACCGCAATTTCGCGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACTTGGTTCTTTGCTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATCTTCTGGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCCAATTTTGTCAAGAAAGTCGCTTTCTCAATGGTCGGTTGAGTTTTTCTTTTCTTTCTTTTGGGGGTCACGGCAATTGGTTGGTAGTTATTAATTGTATTACATCCAAAAACTAATAATTCAATTTCATTACATATATTTCCTGTTTGTTATGAAAAATGACCTCATCCTCAACTCCATATATATATATAAATTTTCTAAAAATTTC

mRNA sequence

CTTCTTCACTTTCATTCCCATAGAGATAAACAAAACTAGAGAGAAAAAAAAAATGGAGGGAGAAATCATAAACTTAACCAAGGTATGGTTAACTGTCTGTATCTCTCTCTCTTACTGCCACGCCGCCGGAAAACTCCTCAATCCCGGTGCTGGCCGCTTCCTCGCCATCGCCCCCGTCGTCCTCCTCTTCTTCCTCCTTCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTTGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCACTGCCGGAGATGGTCCTCTCTCCACTACCCCGCCGCTTCCTCTCCGCCGATTCATAGCCATATCTTGCTTGCCGATCAAGATCCTGGAAAACCCATCGGAGAAATCGGCCCCCAATAATCGAGCGGAAACCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAGAGCTCTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTTCTCGGAATCGAGCTGGAACCGCAATTTCGCGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACTTGGTTCTTTGCTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATCTTCTGGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCCAATTTTGTCAAGAAAGTCGCTTTCTCAATGGTCGGTTGAGTTTTTCTTTTCTTTCTTTTGGGGGTCACGGCAATTGGTTGGTAGTTATTAATTGTATTACATCCAAAAACTAATAATTCAATTTCATTACATATATTTCCTGTTTGTTATGAAAAATGACCTCATCCTCAACTCCATATATATATATAAATTTTCTAAAAATTTC

Coding sequence (CDS)

ATGGAGGGAGAAATCATAAACTTAACCAAGGTATGGTTAACTGTCTGTATCTCTCTCTCTTACTGCCACGCCGCCGGAAAACTCCTCAATCCCGGTGCTGGCCGCTTCCTCGCCATCGCCCCCGTCGTCCTCCTCTTCTTCCTCCTTCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTTGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCACTGCCGGAGATGGTCCTCTCTCCACTACCCCGCCGCTTCCTCTCCGCCGATTCATAGCCATATCTTGCTTGCCGATCAAGATCCTGGAAAACCCATCGGAGAAATCGGCCCCCAATAATCGAGCGGAAACCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAGAGCTCTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTTCTCGGAATCGAGCTGGAACCGCAATTTCGCGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACTTGGTTCTTTGCTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATCTTCTGGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCCAATTTTGTCAAGAAAGTCGCTTTCTCAATGGTCGGTTGA

Protein sequence

MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGGATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG

Homology

[Close]

BLAST of PI0014314 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 158/337 (46.88%), Postives = 218/337 (64.69%), Query Frame = 0


Query: 10  KVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGGATCFFVTWL 69
           K W  V ISL Y     KL+  G  R +   PV L+FF++P  + S+H  G T FF+ WL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ATFKLLLFTAGDGPLSTT-PPLPLRRFIAISCLPIKILENPSEKSAPNNRAETLNEKPKS 129
           A FKLLLF  G GPLS+   PL L  F+A+SCLPIKI  +P      ++   T  E P  
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST--EGP-- 125

Query: 130 LLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGRNLLGIEL 189
            L Y+I+A+ VVL++K Y+YS  L  K+VL +Y+ H+Y  LEIILA T    R +  +EL
Sbjct: 126 -LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 185

Query: 190 EPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLPGVMATFL 249
           EPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ + V+G  ++ +     TF+
Sbjct: 186 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFV 245

Query: 250 VSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAKVSVPVTLV 309
           VS +MHELIF+Y+GR+RP+W++ WFF I+G   TVEI +KK +  + R P  +S  +TL 
Sbjct: 246 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 305

Query: 310 FIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 346
           F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 306 FVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of PI0014314 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-67
Identity = 144/346 (41.62%), Postives = 207/346 (59.83%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           M+ E+ NL KVW++  IS+SYC+     +  GA RFL+++PV+ LF +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
            T FF+TWLA FKL+LF+   GPL    P    RF   +C PIK+ +NP  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
             N  PK  L ++I+  I  +++ LY Y + L P ++L +Y  H+YL +EIIL   +   
Sbjct: 121 --NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK--MLPEKRRLP 300
           PG++A F+VS ++HEL+F+YL R  P  E+T FF +HG+   VE+ +KK   + ++ RL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 345
             VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of PI0014314 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-65
Identity = 136/352 (38.64%), Postives = 211/352 (59.94%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLL-NPGAGRFLAIAPVVLLFFLLPLQLTSIHFG 60
           +E E+   ++VW++   +  YC     +  + GA R L + PVVLLF  LPL+L+S H G
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRA 120
           G T  ++ WLA FKLLLF    GPLS  P L L  FI+ + LPIK  ++PS     N R 
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR- 122

Query: 121 ETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
            TL+ + + ++ +  + +    ++K+Y++ + L   ++  +Y +H YL  EI LA +   
Sbjct: 123 -TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVI 182

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
            R  LG++L PQF +PY +TSLQDFWGRRWNLM ++IL    Y+P  ++ ++ +   W  
Sbjct: 183 ARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEV 242

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPA 300
              ++  F VS +MHE+ F+YL R RP+WE+T FF +HG+   VE+++KK +  K RL  
Sbjct: 243 AGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRR 302

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           +VS  +T+ F+  T  WLF PQ VR  +D++  +EY  + N+ +K    ++G
Sbjct: 303 EVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

[Close]

BLAST of PI0014314 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.9e-65
Identity = 139/348 (39.94%), Postives = 203/348 (58.33%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME E+++L KVW+   IS+SYC+     +  G  R L++ PV +LF +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF++WLA FKL+LF+   GPL   P   L RF+  +C PIK+ +NP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
             N+ PK    ++++     +++ +Y+Y + + P ++L +YS H+YL  EI+LA  +   
Sbjct: 121 --NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
              L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  +MA + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK-MLPEKRRLPA 300
            GV A+FLVS ++HELIF+Y  R  P  E+T FF +HG+    E   K+  L  + ++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAF 348
            VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFF 335

[Close]

BLAST of PI0014314 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-63
Identity = 137/346 (39.60%), Postives = 203/346 (58.67%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME +  NL +VW++  ISLSYC+     L+ G  R L+I PV +LF +LPL L+ +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
            +  F++WLA FKLLLF   +GPL   PP  L RFI  +CLPIKI ++PS  + PN    
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPN---- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
            L+ KP      +++ L++ +++ +Y+Y + L   +VL +Y  H+YL +E++L       
Sbjct: 121 -LHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
             LLG  +EP F +PY +TSLQDFW RRWNLM + +L+  V+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK---MLPEKRRL 300
            GVMA+F VS +MHEL+++Y+ R  P WE+T FF +HG A   EI +K+   + P  R  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLRPPHR-- 300

Query: 301 PAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 344
              VS  V L F+  T +WLF  Q +R  +  +A  E   + +  K
Sbjct: 301 --AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of PI0014314 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 332/352 (94.32%), Postives = 337/352 (95.74%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME EIINLTKVWLTV ISL YCHA  KLLNPG  RFLAIAPVVL+FFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAP-NNRA 120
           ATCFFVTWLATFKLLL TAGDGPLSTTPPLPL RFIAISCLPIKILENPSEKSAP NNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFF IHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFS+VG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of PI0014314 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.9e-166
Identity = 287/350 (82.00%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGEIIN T VWL VC+SL YCH AGK+L+PGAGRFLAI+PVVLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           AT FF+ WLA FKLLLF AG+GPLS TPPLPLRRF+A+SCLPIKIL+NPSEK +P N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
           TLN++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVK+AAKV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           PGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFF IHG ALTVEI++KK+LPEKRRLPA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMV 351
           VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF +V
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of PI0014314 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.2e-165
Identity = 284/350 (81.14%), Postives = 319/350 (91.14%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGE+IN   VWL VC+SL YCH AGK+L PGAGRFLAIAPVV+LFFLLPLQLTS+H GG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF+ WLA FKLLLFTAG+GPLS TPPLPLRRF+A+SCLPIKIL+NPSEK +P N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
           TLN++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVK+AAKV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           PGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFF IHG ALTVEI++KK+LPEKRRLPA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMV 351
           VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF +V
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of PI0014314 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.4e-163
Identity = 287/351 (81.77%), Postives = 313/351 (89.17%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGEI+N  KVWLTVCISL YCHA GKL+ PGAGRFLA+AP+V+LFF LPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF+ WLA FKLLLFTAGDGPLS  PPLPLRRF+A+SCLPIKIL+N  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
               KPKS LNYSI+A+IV LMVKLYDYSEF+HPK+VLFIYSWH+Y LLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           RN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VK+AAKVIGR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           P VMATFLVSAMMHELIF+Y+GRMRPNWE TWFF IHG+ALTVEIM+KK+LPEK RLP  
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFS+VG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of PI0014314 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.4e-163
Identity = 288/351 (82.05%), Postives = 312/351 (88.89%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGEI+N  KVWLTVCISL YCHA GKLL PG GRFLA+AP+V+LFFLLPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF+ WLA FKLLLF AGDGPLS  PPLPLRRF+A+SCLPIKIL+N  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
               KPKS LNYSI+A+IV LMVKLYDYSEF+HPK+VLFIYSWH+Y LLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           RN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VK+AAKVIGR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           P VMATFLVSAMMHELIFYY+GRMRPNWE TWFF IHG+ALTVEIM+KK+LPEK RLP  
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFS+VG
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of PI0014314 vs. NCBI nr
Match: XP_004149740.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 4.1e-184
Identity = 332/352 (94.32%), Postives = 337/352 (95.74%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME EIINLTKVWLTV ISL YCHA  KLLNPG  RFLAIAPVVL+FFLLPLQLTSIHFGG
Sbjct: 1   MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAP-NNRA 120
           ATCFFVTWLATFKLLL TAGDGPLSTTPPLPL RFIAISCLPIKILENPSEKSAP NNRA
Sbjct: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120

Query: 121 ETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFF IHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFS+VG
Sbjct: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 352

[Close]

BLAST of PI0014314 vs. NCBI nr
Match: KGN58563.1 (hypothetical protein Csa_002202 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 4.1e-184
Identity = 332/352 (94.32%), Postives = 337/352 (95.74%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME EIINLTKVWLTV ISL YCHA  KLLNPG  RFLAIAPVVL+FFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAP-NNRA 120
           ATCFFVTWLATFKLLL TAGDGPLSTTPPLPL RFIAISCLPIKILENPSEKSAP NNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFF IHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMVG 352
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFS+VG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of PI0014314 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.5 bits (1534), Expect = 3.0e-166
Identity = 287/350 (82.00%), Postives = 319/350 (91.14%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGEIIN T VWL VC+SL YCH AGK+L PGAGRFLAIAPVVLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           AT FF+ WLA FKLLLF AG+GPLS TPPLPLRRF+A+SCLPIKIL+NPSEK +P N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
           TLN++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVK+AAKV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           PGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFF IHG ALTVEI++KK+LPEKRRLPA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMV 351
           VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF +V
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of PI0014314 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 595.1 bits (1533), Expect = 3.9e-166
Identity = 287/350 (82.00%), Postives = 320/350 (91.43%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGEIIN T VWL VC+SL YCH AGK+L+PGAGRFLAI+PVVLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           AT FF+ WLA FKLLLF AG+GPLS TPPLPLRRF+A+SCLPIKIL+NPSEK +P N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
           TLN++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVK+AAKV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           PGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFF IHG ALTVEI++KK+LPEKRRLPA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMV 351
           VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF +V
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of PI0014314 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 592.4 bits (1526), Expect = 2.5e-165
Identity = 284/350 (81.14%), Postives = 319/350 (91.14%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           MEGE+IN   VWL VC+SL YCH AGK+L PGAGRFLAIAPVV+LFFLLPLQLTS+H GG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF+ WLA FKLLLFTAG+GPLS TPPLPLRRF+A+SCLPIKIL+NPSEK +P N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
           TLN++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVK+AAKV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAK 300
           PGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFF IHG ALTVEI++KK+LPEKRRLPA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSMV 351
           VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF +V
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of PI0014314 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 291.6 bits (745), Expect = 8.5e-79
Identity = 158/337 (46.88%), Postives = 218/337 (64.69%), Query Frame = 0


Query: 10  KVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGGATCFFVTWL 69
           K W  V ISL Y     KL+  G  R +   PV L+FF++P  + S+H  G T FF+ WL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ATFKLLLFTAGDGPLSTT-PPLPLRRFIAISCLPIKILENPSEKSAPNNRAETLNEKPKS 129
           A FKLLLF  G GPLS+   PL L  F+A+SCLPIKI  +P      ++   T  E P  
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST--EGP-- 125

Query: 130 LLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGRNLLGIEL 189
            L Y+I+A+ VVL++K Y+YS  L  K+VL +Y+ H+Y  LEIILA T    R +  +EL
Sbjct: 126 -LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 185

Query: 190 EPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLPGVMATFL 249
           EPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ + V+G  ++ +     TF+
Sbjct: 186 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFV 245

Query: 250 VSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKRRLPAKVSVPVTLV 309
           VS +MHELIF+Y+GR+RP+W++ WFF I+G   TVEI +KK +  + R P  +S  +TL 
Sbjct: 246 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 305

Query: 310 FIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 346
           F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 306 FVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of PI0014314 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.8e-68
Identity = 144/346 (41.62%), Postives = 207/346 (59.83%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           M+ E+ NL KVW++  IS+SYC+     +  GA RFL+++PV+ LF +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
            T FF+TWLA FKL+LF+   GPL    P    RF   +C PIK+ +NP  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
             N  PK  L ++I+  I  +++ LY Y + L P ++L +Y  H+YL +EIIL   +   
Sbjct: 121 --NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK--MLPEKRRLP 300
           PG++A F+VS ++HEL+F+YL R  P  E+T FF +HG+   VE+ +KK   + ++ RL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 345
             VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of PI0014314 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.3e-66
Identity = 139/348 (39.94%), Postives = 203/348 (58.33%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME E+++L KVW+   IS+SYC+     +  G  R L++ PV +LF +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
           +T FF++WLA FKL+LF+   GPL   P   L RF+  +C PIK+ +NP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
             N+ PK    ++++     +++ +Y+Y + + P ++L +YS H+YL  EI+LA  +   
Sbjct: 121 --NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
              L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  +MA + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK-MLPEKRRLPA 300
            GV A+FLVS ++HELIF+Y  R  P  E+T FF +HG+    E   K+  L  + ++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAF 348
            VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFF 335

[Close]

BLAST of PI0014314 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.2e-64
Identity = 137/346 (39.60%), Postives = 203/346 (58.67%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME +  NL +VW++  ISLSYC+     L+ G  R L+I PV +LF +LPL L+ +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLPLRRFIAISCLPIKILENPSEKSAPNNRAE 120
            +  F++WLA FKLLLF   +GPL   PP  L RFI  +CLPIKI ++PS  + PN    
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPN---- 120

Query: 121 TLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFG 180
            L+ KP      +++ L++ +++ +Y+Y + L   +VL +Y  H+YL +E++L       
Sbjct: 121 -LHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPL 240
             LLG  +EP F +PY +TSLQDFW RRWNLM + +L+  V+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKK---MLPEKRRL 300
            GVMA+F VS +MHEL+++Y+ R  P WE+T FF +HG A   EI +K+   + P  R  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLRPPHR-- 300

Query: 301 PAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 344
              VS  V L F+  T +WLF  Q +R  +  +A  E   + +  K
Sbjct: 301 --AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of PI0014314 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.2e-64
Identity = 139/347 (40.06%), Postives = 203/347 (58.50%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLSYCHAAGKLLNPGAGRFLAIAPVVLLFFLLPLQLTSIHFGG 60
           ME E+ N  K+W++  IS+SYC+     +  G  R L++ PV  LF + PL  + +HF G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATCFFVTWLATFKLLLFTAGDGPLSTTPPLP--LRRFIAISCLPIKILENPSEKSAPNNR 120
              FF++WLA FKL+LF+   GPLS   PLP  L RFI I+C PIK  +NP+        
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPN-------- 120

Query: 121 AETLNEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQF 180
              +      +  ++I+ +I V+++++Y+Y ++L P L+L   S H++L LEI+  + + 
Sbjct: 121 ---IQNYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKA 180

Query: 181 FGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWA 240
                LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+PAVY P  +MA + +    A
Sbjct: 181 LVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQA 240

Query: 241 PLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFAIHGIALTVEIMMKKMLPEKR--R 300
              GV A FLVS  +HE++F+YL R  P  E+TWFF +HG+    E+ +KK    +R  R
Sbjct: 241 MFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWR 300

Query: 301 LPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 344
           +   VS  +T+ F+  T+ W FFP  +R  +  R   E     +FVK
Sbjct: 301 VSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

The following BLAST results are available for this feature:

BLAST of PI0014314 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.2e-77	46.88	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ76	2.5e-67	41.62	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	1.1e-65	38.64	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	8.9e-65	39.94	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	1.7e-63	39.60	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]

BLAST of PI0014314 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9S2	2.0e-184	94.32	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]
A0A6J1J5C4	1.9e-166	82.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	1.2e-165	81.14	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	7.4e-163	81.77	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	7.4e-163	82.05	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of PI0014314 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149740.1	4.1e-184	94.32	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus]	[more]
KGN58563.1	4.1e-184	94.32	hypothetical protein Csa_002202 [Cucumis sativus]	[more]
XP_023527814.1	3.0e-166	82.00	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	3.9e-166	82.00	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	2.5e-165	81.14	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]

BLAST of PI0014314 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	8.5e-79	46.88	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	1.8e-68	41.62	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	6.3e-66	39.94	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.2e-64	39.60	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.2e-64	40.06	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 189..275 e-value: 6.0E-15 score: 55.3
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..351 e-value: 1.3E-116 score: 387.5
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..346
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014314.1	PI0014314.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity