PI0014247 (gene) Melon (PI 482460) v1

Overview


Name	PI0014247
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	chr08: 6938157 .. 6939718 (-)
RNA-Seq Expression	PI0014247
Synteny	PI0014247

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAGAGACAGAGGCTACATTATTGGAGACTTCGAGCTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGGTGAGACATGAAAAAAGAAGATCTTGTTATCAATAAATCCATGAGAAATAATAGCCCTGACCAGGTATAGTCTCATGCTAGATTGTCTCTAAACTTGTTTACTTTGATTTTTACTAAGGAGCTCCATCTTACAACTCTGCACCCAAACAAAGGATTCCTAATTCAATTCTAACATATTAACTCAAAACTTCTAACTCAATTTAGTATCTCAAACATCCCTCAATTGTATAAAACAAGGTCTCAGTTACTCATGAAATTATAGTGTATTTGAACACGTTTAAACTTTACTTTGATAAATTTCCTTAGGAGAAGTTTCTATTTGAGTCATTCTCAAATACTTTGACATGGAAATTACTTAACTATCTATCATTGAGTAGTATATTATACTATTTCATAAATATCTACCAATTTTATGTTAAGTTTGGTTAGATGGATGTATAGGTTATTTGTTAATCCTTTTATTTGTTCCCCTCTAATTTATGACTCTTTTACACATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGAAAATGTTTTTAGAGCAATATTGGTTGTTCAAGAAAAATTGTCCTCATTTGTCGTTCTTGTATCTGCAAAGCACCTAAATTCCATTTGGAGGTTTTCCAGGTAAAAATTAAATCTTTGAATAATTTATGGAGCCTTTTAATGTCCAGATAGATTATAGTAGTATTATCACTATCACATGCAAAAAATTTGATGAGAACTAGAGAGGATCTTCTATGCTTCTATCCTAAAACTAGTTCTCATACTTTTATTGTCTCTCACATCGACAGTGAAAATGAAGGGGGGTGCTAAAGTGCTTTGCCTGCACTCTTTTTTTACAGAAGTTAATAATTACCTCTTTGTTACTTTTTGATGCAGGAAGCAGAATTTTTGGTTAACATCAAAGATCACGTTCTCATTCTCGAGCACCAGTTGTTGACAAATGAAGAGAAGAATACGTTGCTGGAGAGGTATATGGTGAAAGATACACAAGTGAGTTTCATTTTATTAGATCTATTTTCTTGAAATACAAGGAACATTGTGAGAATTATCTTTCATAAATATGTTGAAATGATCTTGGTAGATTGGCCCGTGGTGAAGAAGGGCTTTGTAAATAATAAAAATTTTATGAATGAGCTCAAGCCATGATGAGATTTAATATTCTCTGAGTTACATTGACCATCACATATAGTAGGGTTAGGTAGTTGTTTCGTTAGAATAGTCGAGATACATGCAACTTGGCTCTAACACTCTTGGATACATGTTGAAATGTGTATAGGTTAGTTCCTAATGTGATTTCATATTTTTTCTTATCTACCTCACATGCAGGTTACTGATCCAATTGCAAGATATTATGGAATGAAGCGTGGACAAGTAGTGAAGATCATCCGACCAAGCGAAACTGCGGGAAGATATGTGACATATAGATATGTTGTTTGA

mRNA sequence

ATGTTGAGAGACAGAGGCTACATTATTGGAGACTTCGAGCTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGAAAATGTTTTTAGAGCAATATTGGTTGTTCAAGAAAAATTGTCCTCATTTGTCGTTCTTGAAGCAGAATTTTTGGTTAACATCAAAGATCACGTTCTCATTCTCGAGCACCAGTTGTTGACAAATGAAGAGAAGAATACGTTGCTGGAGAGGTATATGGTGAAAGATACACAAGTTACTGATCCAATTGCAAGATATTATGGAATGAAGCGTGGACAAGTAGTGAAGATCATCCGACCAAGCGAAACTGCGGGAAGATATGTGACATATAGATATGTTGTTTGA

Coding sequence (CDS)

ATGTTGAGAGACAGAGGCTACATTATTGGAGACTTCGAGCTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGAAAATGTTTTTAGAGCAATATTGGTTGTTCAAGAAAAATTGTCCTCATTTGTCGTTCTTGAAGCAGAATTTTTGGTTAACATCAAAGATCACGTTCTCATTCTCGAGCACCAGTTGTTGACAAATGAAGAGAAGAATACGTTGCTGGAGAGGTATATGGTGAAAGATACACAAGTTACTGATCCAATTGCAAGATATTATGGAATGAAGCGTGGACAAGTAGTGAAGATCATCCGACCAAGCGAAACTGCGGGAAGATATGTGACATATAGATATGTTGTTTGA

Protein sequence

MLRDRGYIIGDFELAMSREQFKNQYEPKVSVKTVRTYVNRMKSENVFRAILVVQEKLSSFVVLEAEFLVNIKDHVLILEHQLLTNEEKNTLLERYMVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV

Homology

[Close]

BLAST of PI0014247 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-41
Identity = 103/186 (55.38%), Postives = 118/186 (63.44%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRGY I D EL M+++QF  ++                             E KV V
Sbjct: 20  MLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSDQLYIFFPDEAKVGV 79

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF---------------VVLEAEFLVNIKDHVL 120
           KT++ Y NRMKSENVFRAILVVQ+ L+ F               V  EAE LVNIK+HVL
Sbjct: 80  KTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFHLEVFQEAEMLVNIKEHVL 139

Query: 121 ILEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYV 138
           + EHQ+LT EEK TLLERY VK+T     QVTDPIARY+G+KRGQVVKIIRPSETAGRYV
Sbjct: 140 VPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQVVKIIRPSETAGRYV 199

[Close]

BLAST of PI0014247 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.4e-26
Identity = 72/190 (37.89%), Postives = 105/190 (55.26%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY----------------------------------E 60
           +  DRGY++   EL  + E+FK Q+                                  E
Sbjct: 20  LCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEE 79

Query: 61  PKVSVKTVRTYVNRMKSENVFRAILVVQEKLS------------SFVV---LEAEFLVNI 120
           PKV +KT++ Y  RM+ EN+ RA++VVQ+ ++             +V+   L+ E L+NI
Sbjct: 80  PKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYVLEQFLQQELLINI 139

Query: 121 KDHVLILEHQLLTNEEKNTLLERYMVKDTQVT-----DPIARYYGMKRGQVVKIIRPSET 137
            +H L+ EH ++T EE   LL RY ++++Q+      DP+ARY+G+KRGQVVKIIRPSET
Sbjct: 140 TEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYFGIKRGQVVKIIRPSET 199

[Close]

BLAST of PI0014247 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.4e-26
Identity = 72/190 (37.89%), Postives = 105/190 (55.26%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY----------------------------------E 60
           +  DRGY++   EL  + E+FK Q+                                  E
Sbjct: 20  LCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEE 79

Query: 61  PKVSVKTVRTYVNRMKSENVFRAILVVQEKLS------------SFVV---LEAEFLVNI 120
           PKV +KT++ Y  RM+ EN+ RA++VVQ+ ++             +V+   L+ E L+NI
Sbjct: 80  PKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYVLEQFLQQELLINI 139

Query: 121 KDHVLILEHQLLTNEEKNTLLERYMVKDTQVT-----DPIARYYGMKRGQVVKIIRPSET 137
            +H L+ EH ++T EE   LL RY ++++Q+      DP+ARY+G+KRGQVVKIIRPSET
Sbjct: 140 TEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYFGIKRGQVVKIIRPSET 199

[Close]

BLAST of PI0014247 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-25
Identity = 72/190 (37.89%), Postives = 103/190 (54.21%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY----------------------------------E 60
           +  DRGY++   EL  + E+F+ Q+                                  E
Sbjct: 20  LCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEE 79

Query: 61  PKVSVKTVRTYVNRMKSENVFRAILVVQEKLS-----SFV----------VLEAEFLVNI 120
           PKV +KT++ Y  RM+ EN+ RA++VVQ+ ++     S V           L+ E L+NI
Sbjct: 80  PKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINI 139

Query: 121 KDHVLILEHQLLTNEEKNTLLERYMVKDTQVT-----DPIARYYGMKRGQVVKIIRPSET 137
            +H L+ EH ++T EE   LL RY +++ Q+      DP+ARY+G+KRGQVVKIIRPSET
Sbjct: 140 TEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSET 199

[Close]

BLAST of PI0014247 vs. ExPASy Swiss-Prot
Match: Q9N5K2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans OX=6239 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.7e-25
Identity = 73/191 (38.22%), Postives = 101/191 (52.88%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY----------------------------------E 60
           M+ DRGY++   EL    E FK QY                                  +
Sbjct: 21  MVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLTILVAHNDDPADQMFVFFPED 80

Query: 61  PKVSVKTVRTYVNRMKSENVFRAILVVQEKLSSFV---------------VLEAEFLVNI 120
            K+ +KT++    +M+ +N+ RAI+VVQ  ++                   LEAE +VNI
Sbjct: 81  AKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDMAPKYMLEHFLEAELMVNI 140

Query: 121 KDHVLILEHQLLTNEEKNTLLERYMVKDTQV-----TDPIARYYGMKRGQVVKIIRPSET 138
            +H L+ EH ++T EEK  LL RY +KD+Q+      DP+ARY+G++RGQVVKIIRPSET
Sbjct: 141 TEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVARYFGLRRGQVVKIIRPSET 200

[Close]

BLAST of PI0014247 vs. ExPASy TrEMBL
Match: A0A1S4E290 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498269 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.4e-53
Identity = 125/180 (69.44%), Postives = 131/180 (72.78%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQ+                             EPKVSV
Sbjct: 21  MLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV 138
            EHQLLTNEEK TLLERY VKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 141 PEHQLLTNEEKKTLLERYTVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV 200

[Close]

BLAST of PI0014247 vs. ExPASy TrEMBL
Match: A0A0A0LYM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G702030 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 6.0e-52
Identity = 125/185 (67.57%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQY                             EPKVSV
Sbjct: 21  MLRDRGFVVGDFELAMSREQFKNQYGETMKKEDLVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            EHQLLTNE+K TLLERY VKDT     QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT
Sbjct: 141 PEHQLLTNEDKKTLLERYTVKDTQLPRMQVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 200

[Close]

BLAST of PI0014247 vs. ExPASy TrEMBL
Match: A0A1S3C8T2 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498269 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.0e-51
Identity = 125/185 (67.57%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQ+                             EPKVSV
Sbjct: 21  MLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            EHQLLTNEEK TLLERY VKDT     QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT
Sbjct: 141 PEHQLLTNEEKKTLLERYTVKDTQLPRMQVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 200

[Close]

BLAST of PI0014247 vs. ExPASy TrEMBL
Match: A0A5A7TIR4 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00120 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.0e-51
Identity = 125/185 (67.57%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQ+                             EPKVSV
Sbjct: 21  MLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            EHQLLTNEEK TLLERY VKDT     QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT
Sbjct: 141 PEHQLLTNEEKKTLLERYTVKDTQLPRMQVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 200

[Close]

BLAST of PI0014247 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 6.4e-46
Identity = 112/186 (60.22%), Postives = 125/186 (67.20%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           ML+DRGY +GDFE+ MSREQFKN+Y                             EPKV V
Sbjct: 21  MLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRNDSSDQIYIFFPEEPKVGV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF---------------VVLEAEFLVNIKDHVL 120
           KT++TY NRMKSENVFRAILVVQ+ L+ F               V  EAE LVNIKDHVL
Sbjct: 81  KTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTKFHLEVFQEAELLVNIKDHVL 140

Query: 121 ILEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYV 138
           + EHQLL+NEEK TLLERY VK+T     QVTDP+ARYYG+KRGQVVKIIRPSETAGRYV
Sbjct: 141 VPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKIIRPSETAGRYV 200

[Close]

BLAST of PI0014247 vs. NCBI nr
Match: XP_016902349.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cucumis melo])

HSP 1 Score: 218.8 bits (556), Expect = 2.9e-53
Identity = 125/180 (69.44%), Postives = 131/180 (72.78%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQ+                             EPKVSV
Sbjct: 21  MLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV 138
            EHQLLTNEEK TLLERY VKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 141 PEHQLLTNEEKKTLLERYTVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYVV 200

[Close]

BLAST of PI0014247 vs. NCBI nr
Match: XP_004145446.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN66878.1 hypothetical protein Csa_007321 [Cucumis sativus])

HSP 1 Score: 213.4 bits (542), Expect = 1.2e-51
Identity = 125/185 (67.57%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQY                             EPKVSV
Sbjct: 21  MLRDRGFVVGDFELAMSREQFKNQYGETMKKEDLVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            EHQLLTNE+K TLLERY VKDT     QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT
Sbjct: 141 PEHQLLTNEDKKTLLERYTVKDTQLPRMQVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 200

[Close]

BLAST of PI0014247 vs. NCBI nr
Match: XP_008459046.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >XP_008459047.1 PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >XP_008459048.1 PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >KAA0043210.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa] >TYK12258.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 212.6 bits (540), Expect = 2.1e-51
Identity = 125/185 (67.57%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRG+++GDFELAMSREQFKNQ+                             EPKVSV
Sbjct: 21  MLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSMRNNSSDQIYVFFPEEPKVSV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF--------------VVLEAEFLVNIKDHVLI 120
           KTVRTY+NRMKS+NVFRAILVVQEKLSSF              V  EAE LVNIKDHVLI
Sbjct: 81  KTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKFHLEVFQEAELLVNIKDHVLI 140

Query: 121 LEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            EHQLLTNEEK TLLERY VKDT     QVTDPIARYYGMKRGQVVKIIRPSETAGRYVT
Sbjct: 141 PEHQLLTNEEKKTLLERYTVKDTQLPRMQVTDPIARYYGMKRGQVVKIIRPSETAGRYVT 200

[Close]

BLAST of PI0014247 vs. NCBI nr
Match: KAG7014568.1 (DNA-directed RNA polymerases II and IV subunit 5A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 194.9 bits (494), Expect = 4.6e-46
Identity = 109/181 (60.22%), Postives = 124/181 (68.51%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           ML+DRGY +GDFE+ MSREQFK++Y                             E KV V
Sbjct: 135 MLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAKRNDSSDQIYVFFPEEQKVGV 194

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF---------------VVLEAEFLVNIKDHVL 120
           KT++TY NRMK+ENVFRAILVVQ+ L+ F               V  EAE LVN+KDHVL
Sbjct: 195 KTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSKFHLEVFQEAELLVNVKDHVL 254

Query: 121 ILEHQLLTNEEKNTLLERYMVKDTQVTDPIARYYGMKRGQVVKIIRPSETAGRYVTYRYV 138
           + EHQLLTNEEK TLLERY VK+TQVTDP+ARYYG+KRGQVVKIIRPSETAGRYVTYRYV
Sbjct: 255 VPEHQLLTNEEKKTLLERYTVKETQVTDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYV 314

[Close]

BLAST of PI0014247 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.4 bits (490), Expect = 1.3e-45
Identity = 112/186 (60.22%), Postives = 125/186 (67.20%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           ML+DRGY +GDFE+ MSREQFKN+Y                             EPKV V
Sbjct: 21  MLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRNDSSDQIYIFFPEEPKVGV 80

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF---------------VVLEAEFLVNIKDHVL 120
           KT++TY NRMKSENVFRAILVVQ+ L+ F               V  EAE LVNIKDHVL
Sbjct: 81  KTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTKFHLEVFQEAELLVNIKDHVL 140

Query: 121 ILEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYV 138
           + EHQLL+NEEK TLLERY VK+T     QVTDP+ARYYG+KRGQVVKIIRPSETAGRYV
Sbjct: 141 VPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKIIRPSETAGRYV 200

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BLAST of PI0014247 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 170.6 bits (431), Expect = 8.6e-43
Identity = 103/186 (55.38%), Postives = 118/186 (63.44%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------------------EPKVSV 60
           MLRDRGY I D EL M+++QF  ++                             E KV V
Sbjct: 20  MLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSDQLYIFFPDEAKVGV 79

Query: 61  KTVRTYVNRMKSENVFRAILVVQEKLSSF---------------VVLEAEFLVNIKDHVL 120
           KT++ Y NRMKSENVFRAILVVQ+ L+ F               V  EAE LVNIK+HVL
Sbjct: 80  KTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFHLEVFQEAEMLVNIKEHVL 139

Query: 121 ILEHQLLTNEEKNTLLERYMVKDT-----QVTDPIARYYGMKRGQVVKIIRPSETAGRYV 138
           + EHQ+LT EEK TLLERY VK+T     QVTDPIARY+G+KRGQVVKIIRPSETAGRYV
Sbjct: 140 VPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQVVKIIRPSETAGRYV 199

[Close]

BLAST of PI0014247 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 85.9 bits (211), Expect = 2.8e-17
Identity = 65/190 (34.21%), Postives = 92/190 (48.42%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY--------------------------------EPK 60
           MLRDRGY I + +L + RE+F  ++                                 PK
Sbjct: 21  MLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKGPNPADKIYVFYPEGPK 80

Query: 61  VSVKTVRTYVN-RMKSENVFRAILVV---------------QEKLSSFVVLEAEFLVNIK 120
           V V  ++  V  +M+ + V R I+VV                + L+  V  EAE + NI 
Sbjct: 81  VGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKMLTIEVFEEAELVTNIT 140

Query: 121 DHVLILEHQLLTNEEKNTLLERYMVKDTQ-----VTDPIARYYGMKRGQVVKIIRPSETA 138
           +H L+ ++ +L ++ K  LL  Y V+DTQ     VTDP+ARYYG+KRGQVVKI R   T+
Sbjct: 141 EHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYYGLKRGQVVKIRRSDATS 200

[Close]

BLAST of PI0014247 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 72.4 bits (176), Expect = 3.2e-13
Identity = 55/184 (29.89%), Postives = 88/184 (47.83%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQYEPKVSVKTVR----------------------TYV 60
           MLRDRGY + D ++ +S +QF+  Y     V  +R                        V
Sbjct: 34  MLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFDSSKKISVVFCGTGIVKV 93

Query: 61  NRMK--------SENVFRAILVVQEKLSSFVVLEAE-------------FLVNIKDHVLI 120
           N M+         EN+   ILV+Q  +++  +   E              LVN+  HVL 
Sbjct: 94  NAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFSFKVELFEITDLLVNVSKHVLR 153

Query: 121 LEHQLLTNEEKNTLLERYMVKDTQV-----TDPIARYYGMKRGQVVKIIRPSETAGRYVT 137
            +HQ+L ++EK +LL+++ +++ Q+      DPI RYYG++ GQV+K+    E +  +VT
Sbjct: 154 PKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGLETGQVMKVTYKDELSESHVT 213

[Close]

BLAST of PI0014247 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 65.9 bits (159), Expect = 3.0e-11
Identity = 53/185 (28.65%), Postives = 89/185 (48.11%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQY-----------------EPK------------VSV 60
           MLRDRGY + + EL+++  +F++ +                 +PK            ++V
Sbjct: 49  MLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKKKILVVFMGTEPITV 108

Query: 61  KTVRT-YVNRMKSENVFRAILVVQEKLSSFV---------VLEA----EFLVNIKDHVLI 120
           K+VR  ++    +  +   ILV+Q K++ F           +E     + LVNI  H+  
Sbjct: 109 KSVRALHIQISNNVGLHAMILVLQSKMNHFAQKALTTFPFTVETFPIEDLLVNITKHIQQ 168

Query: 121 LEHQLLTNEEKNTLLERYMVKDTQV-----TDPIARYYGMKRGQVVKIIRPSETAGRYVT 138
            + ++L  EEK  LL ++ ++D Q+      D   RYYG+K+ QVVKI    E  G +VT
Sbjct: 169 PKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKKQVVKITYSKEPVGDFVT 228

[Close]

BLAST of PI0014247 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 64.3 bits (155), Expect = 8.7e-11
Identity = 54/184 (29.35%), Postives = 85/184 (46.20%), Query Frame = 0


Query: 1   MLRDRGYIIGDFELAMSREQFKNQYEPK-----------------------------VSV 60
           MLRDRGY + D ++ +S   F+  Y  +                             V V
Sbjct: 38  MLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRSDSTKKVKIVFFGTSMVKV 97

Query: 61  KTVRTYV-NRMKSENVFRAILVVQE----------KLSSF---VVLEAEFLVNIKDHVLI 120
             +R+ V + +  E +   ILV+Q           +L SF   +    + LVNI  H L 
Sbjct: 98  NAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFSFKVEIFQITDLLVNITKHSLK 157

Query: 121 LEHQLLTNEEKNTLLERYMVKDTQV-----TDPIARYYGMKRGQVVKIIRPSETAGRYVT 137
            +HQ+L +EEK TLL+++ +++ Q+      D I RYYG+++GQVVK+    E    +V 
Sbjct: 158 PQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGLEKGQVVKVNYRGELTESHVA 217

The following BLAST results are available for this feature:

BLAST of PI0014247 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	1.2e-41	55.38	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	5.4e-26	37.89	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	5.4e-26	37.89	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q5R587	2.1e-25	37.89	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q9N5K2	2.7e-25	38.22	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis ele...	[more]

BLAST of PI0014247 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E290	1.4e-53	69.44	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis mel...	[more]
A0A0A0LYM6	6.0e-52	67.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G702030 PE=3 SV=1	[more]
A0A1S3C8T2	1.0e-51	67.57	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A5A7TIR4	1.0e-51	67.57	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A6J1KT45	6.4e-46	60.22	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]

BLAST of PI0014247 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016902349.1	2.9e-53	69.44	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cu...	[more]
XP_004145446.1	1.2e-51	67.57	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN66878.1 ...	[more]
XP_008459046.1	2.1e-51	67.57	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cu...	[more]
KAG7014568.1	4.6e-46	60.22	DNA-directed RNA polymerases II and IV subunit 5A, partial [Cucurbita argyrosper...	[more]
XP_022959553.1	1.3e-45	60.22	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]

BLAST of PI0014247 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	8.6e-43	55.38	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	2.8e-17	34.21	RNA polymerase II fifth largest subunit, C	[more]
AT2G41340.1	3.2e-13	29.89	RNA polymerase II fifth largest subunit, D	[more]
AT3G54490.1	3.0e-11	28.65	RNA polymerase II fifth largest subunit, E	[more]
AT3G57080.1	8.7e-11	29.35	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 69..136 e-value: 3.4E-24 score: 84.5
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 64..137 e-value: 3.4E-23 score: 83.1
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 67..137
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 26..137
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..25
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 26..137 coord: 1..25
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 1..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0014247.1	PI0014247.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity