PI0014163 (gene) Melon (PI 482460) v1

Overview
NamePI0014163
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPhosphoenolpyruvate carboxykinase (ATP)
Locationchr08: 20664441 .. 20667868 (-)
RNA-Seq ExpressionPI0014163
SyntenyPI0014163
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCATCGAAACTCAACCACTCCCTCTCCCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACATTCACACTTTTGATTTCATTCATCTCATAACCCTTCTTTGTCAAACTTCTGTACGTCTACATTTCTCCTATTCCCTCTTTTTTAATTTCTAAACTATTATTCCAATTCTTAACATGATACTTAAGATCAATGAATTTTAATTTCCATCTGTTTTTTTTTATAGGATTGACATGGCTGATTCAGTCATATCCAACGGCAATGGCTTTGCCAAAAAAATTCAAACGTCGACGAAGCAAGGGAATGGAGTTTGCCATGATGACAGCTCGTCGCCGATCAAAGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGGTCCACGCCGAGCACTCCCATCGGCTCTGCTCCGACAGCTTTCTCTTTAATGGCTGAAGAGGATCGCCAGCGGCAACAACTTCAGTCAATCAGGTACCATGCAACATCACGTACATTAATTCATGGTAATTTCATATACTAAAAAACATAATACTATGGGGCTTGGGAAATGTGCCGATAGTATCCATCAAATCGTGACATGTATAATTTATTTAAAGTTATGTTTTATATATGTTTTAAATTATAAGGTTTATTAATCTGGTTGGAAATGGGAATCCAGTGCATCGTTAGCATCATTGACGAGAGAGACGGGACCAAAGGTGGTGAAAGGAGACCCGGCTCGAAAAGAAACAAGTACAAATGTTGCACACGTGGTGCATCATCAACTCGCACCCACCATTAGCATCAGCGATAGCTCAATGAAATTTACACACGTCCTCTACAATCTCTCCCCTGCAGGTCTAAAAAAATACACATTTAATATTTAATTTGATATATTTAACTTGGTCATATTTAAGCTCAATAAAAACGATGTCGTGTCGTTTTGACAGAGTTGTACGAGCAAGCCATAAGATACGAAAAGGGCTCGTTTTTTACCTCAACAGGAGCACTGGCGACTCTTTCTGGAGCCAAGACCGGGCGATCTCCTAGAGACAAGCGAGTTGTTAAGGATGAGAATACCGAAAGAGAACTCTGGTGGGGAAAGTGAGTATTATTATCCTTATGAAAACATCGTTATTTTCCACTTTTTTTTTCCTCTTCCTGTTAGACTATATTTAATCCATGTTTTTTTTTCTTGTTTCGAGCAACAGGGGATCGCCAAACATTGAAATGGACGAGCATACATTCATGATAAACAGAGAAAGAGCTGTGGATTACTTGAACTCTCTGGACAAAGTCTTCGTCAACGATCAATTCTTGAACTGGGATCCAACAAACAAAATCAAAGTTCGAATTGTGTCTGCCAGGGCTTACCACTCGCTCTTTATGCACAACATGTAAGCAAAAGAAAAAAACAAAGGGTACAAAAATTCTAAAAGGGGGGTTGATAATGACAAATTATTGTTTATGGGGATTTTTTTGGGTAGGTGCATTCGACCAACGGAGGAAGAGTTGGAGAATTTTGGGACACCGGATTTCACGATATACAATGCTGGACAGTTCCCTTGCAACCGTTTTACACATTACATGACATCTTCAACTAGCATAGATGTGAATTTGGCAAGAAAAGAAATGGTGATCCTTGGAACACAGTATGCAGGAGAGATGAAGAAAGGATTGTTCAGTTTGATGCATTATCTCATGCCCAAACGACATATTCTCTCCTTGCATTCTGGTTGTAATATGGGAAAAGATGGTGATGTTGCACTCTTCTTTGGGTTATCAGGTATACAAATATACCTAAACTAGACTTATAATTAAACATTTAAACTAAATTTAATTTGTTTCTTAATTTATTTGCTTTGTGTTTAGGTACTGGAAAGACGACTTTATCGACAGATCACAACCGTTACTTGATTGGAGACGATGAACATTGTTGGGGCAACGATGGTGTCTCAAACATTGAAGGAGGTTGTTATGCTAAGTGCATTGATCTTTCCCGAGAGAAAGAGCCTGATATCTGGAACGCTATCAAGTTTGGAACTGGTAATGATAATAGTACTAATAATACTTTAAATTATAAATAATAAACTAATGTTTTGAATTAATTAAACATTGACATTTTTAAAAAATGGTTTATTTGGATTGACAGTGTTGGAGAACGTAGTATTCGATGAGCATACTAGAGAGGTTGACTATTCTGACAAATCAGTAACAGGTACAAACTAACAAATTAATTAAACTTATTAGAAGTACAATTAATAACAACCATAACTAGAATTATAAATAATAAATTATTTCATGATACAGAGAACACTCGAGCGGCATACCCAATCGAGTACATACCTAACGCTCTGATACCATGCGTGGGGCCACATCCGAAGAACGTTATATTATTGGCTTGTGATGCATTTGGAGTGCTTCCTCCTGTGAGCAAGCTCAGTTTGGCTCAAACCATGTATCATTTCATCAGTGGATATACCGCATTGGTAAAAACACATACACAGAATAGATTAAACGTTTTTTTTTACTGTGGGGATATTACCGAGGGTTAAAATGAAGTGAAATTGGGAAATTGTAGGTGGCTGGGACAGAAGATGGTATAAAGGAGCCACAAGCGACATTCTCGGCTTGCTTTGGAGCGGCTTTCATAATGTTACATCCCACAAAGTACGCCGCTATGCTCGCTCAAAAGATGCAGAACCACGGCGCTACCGGCTGGCTCGTTAACACTGGCTGGTCAAGCGGCCGATATGGCTACGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAACTTCTCAATGCGGATTATTATAAAACCGAAGTCTTTGGTCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCTGAGATTTTGGACCCAATGAACACGGTAAGAATAAAAAAATGACATTATTTATATGGGTAAGTAAATCTCTTTCAATTTATCATTGATTATTAATTGGTTTTTTCTTTGGCAGTGGTCGGATAAGGATGCGTATAATGAAACGTTGCTGAAATTGGCGAGTCTCTTCAAGAACAATTTTGAGACATTTACCAGTTACAAGATTGGTGAAGACAATAGCTTGACAGAGGAAATCCTTGCTGCTGGTCCGATATTTTAAAACAAAAAAGAAAAAAAAAGAAAAAAAGGATTTGCAGTCATGGAAAATGCTTTGTCTATTATTGCTTTTGTTTCTTTTCTTTTTTGCTCATTGTATTTATTTTATTTTTTAATGCCTAATTGTTGGAAGCTATGTTGCTTTCCATTTTCATTTGTTTTAGAGGCAGAGAGTTGGGAAAAAGGAGAGATGTAGTTGGATCTGGTTTAATGTGGTGTAAAGAGACCAACTAGAGTTCAACATATTTTATGTATGCAAGTTAATATATATGCTGCAAATTCTGAAGAA

mRNA sequence

CATTCATCGAAACTCAACCACTCCCTCTCCCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACATTCACACTTTTGATTTCATTCATCTCATAACCCTTCTTTGTCAAACTTCTGATTGACATGGCTGATTCAGTCATATCCAACGGCAATGGCTTTGCCAAAAAAATTCAAACGTCGACGAAGCAAGGGAATGGAGTTTGCCATGATGACAGCTCGTCGCCGATCAAAGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGGTCCACGCCGAGCACTCCCATCGGCTCTGCTCCGACAGCTTTCTCTTTAATGGCTGAAGAGGATCGCCAGCGGCAACAACTTCAGTCAATCAGTGCATCGTTAGCATCATTGACGAGAGAGACGGGACCAAAGGTGGTGAAAGGAGACCCGGCTCGAAAAGAAACAAGTACAAATGTTGCACACGTGGTGCATCATCAACTCGCACCCACCATTAGCATCAGCGATAGCTCAATGAAATTTACACACGTCCTCTACAATCTCTCCCCTGCAGAGTTGTACGAGCAAGCCATAAGATACGAAAAGGGCTCGTTTTTTACCTCAACAGGAGCACTGGCGACTCTTTCTGGAGCCAAGACCGGGCGATCTCCTAGAGACAAGCGAGTTGTTAAGGATGAGAATACCGAAAGAGAACTCTGGTGGGGAAAGGGATCGCCAAACATTGAAATGGACGAGCATACATTCATGATAAACAGAGAAAGAGCTGTGGATTACTTGAACTCTCTGGACAAAGTCTTCGTCAACGATCAATTCTTGAACTGGGATCCAACAAACAAAATCAAAGTTCGAATTGTGTCTGCCAGGGCTTACCACTCGCTCTTTATGCACAACATGTGCATTCGACCAACGGAGGAAGAGTTGGAGAATTTTGGGACACCGGATTTCACGATATACAATGCTGGACAGTTCCCTTGCAACCGTTTTACACATTACATGACATCTTCAACTAGCATAGATGTGAATTTGGCAAGAAAAGAAATGGTGATCCTTGGAACACAGTATGCAGGAGAGATGAAGAAAGGATTGTTCAGTTTGATGCATTATCTCATGCCCAAACGACATATTCTCTCCTTGCATTCTGGTTGTAATATGGGAAAAGATGGTGATGTTGCACTCTTCTTTGGGTTATCAGGTACTGGAAAGACGACTTTATCGACAGATCACAACCGTTACTTGATTGGAGACGATGAACATTGTTGGGGCAACGATGGTGTCTCAAACATTGAAGGAGGTTGTTATGCTAAGTGCATTGATCTTTCCCGAGAGAAAGAGCCTGATATCTGGAACGCTATCAAGTTTGGAACTGTGTTGGAGAACGTAGTATTCGATGAGCATACTAGAGAGGTTGACTATTCTGACAAATCAGTAACAGAGAACACTCGAGCGGCATACCCAATCGAGTACATACCTAACGCTCTGATACCATGCGTGGGGCCACATCCGAAGAACGTTATATTATTGGCTTGTGATGCATTTGGAGTGCTTCCTCCTGTGAGCAAGCTCAGTTTGGCTCAAACCATGTATCATTTCATCAGTGGATATACCGCATTGGTGGCTGGGACAGAAGATGGTATAAAGGAGCCACAAGCGACATTCTCGGCTTGCTTTGGAGCGGCTTTCATAATGTTACATCCCACAAAGTACGCCGCTATGCTCGCTCAAAAGATGCAGAACCACGGCGCTACCGGCTGGCTCGTTAACACTGGCTGGTCAAGCGGCCGATATGGCTACGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAACTTCTCAATGCGGATTATTATAAAACCGAAGTCTTTGGTCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCTGAGATTTTGGACCCAATGAACACGTGGTCGGATAAGGATGCGTATAATGAAACGTTGCTGAAATTGGCGAGTCTCTTCAAGAACAATTTTGAGACATTTACCAGTTACAAGATTGGTGAAGACAATAGCTTGACAGAGGAAATCCTTGCTGCTGGTCCGATATTTTAAAACAAAAAAGAAAAAAAAAGAAAAAAAGGATTTGCAGTCATGGAAAATGCTTTGTCTATTATTGCTTTTGTTTCTTTTCTTTTTTGCTCATTGTATTTATTTTATTTTTTAATGCCTAATTGTTGGAAGCTATGTTGCTTTCCATTTTCATTTGTTTTAGAGGCAGAGAGTTGGGAAAAAGGAGAGATGTAGTTGGATCTGGTTTAATGTGGTGTAAAGAGACCAACTAGAGTTCAACATATTTTATGTATGCAAGTTAATATATATGCTGCAAATTCTGAAGAA

Coding sequence (CDS)

ATGGCTGATTCAGTCATATCCAACGGCAATGGCTTTGCCAAAAAAATTCAAACGTCGACGAAGCAAGGGAATGGAGTTTGCCATGATGACAGCTCGTCGCCGATCAAAGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGGTCCACGCCGAGCACTCCCATCGGCTCTGCTCCGACAGCTTTCTCTTTAATGGCTGAAGAGGATCGCCAGCGGCAACAACTTCAGTCAATCAGTGCATCGTTAGCATCATTGACGAGAGAGACGGGACCAAAGGTGGTGAAAGGAGACCCGGCTCGAAAAGAAACAAGTACAAATGTTGCACACGTGGTGCATCATCAACTCGCACCCACCATTAGCATCAGCGATAGCTCAATGAAATTTACACACGTCCTCTACAATCTCTCCCCTGCAGAGTTGTACGAGCAAGCCATAAGATACGAAAAGGGCTCGTTTTTTACCTCAACAGGAGCACTGGCGACTCTTTCTGGAGCCAAGACCGGGCGATCTCCTAGAGACAAGCGAGTTGTTAAGGATGAGAATACCGAAAGAGAACTCTGGTGGGGAAAGGGATCGCCAAACATTGAAATGGACGAGCATACATTCATGATAAACAGAGAAAGAGCTGTGGATTACTTGAACTCTCTGGACAAAGTCTTCGTCAACGATCAATTCTTGAACTGGGATCCAACAAACAAAATCAAAGTTCGAATTGTGTCTGCCAGGGCTTACCACTCGCTCTTTATGCACAACATGTGCATTCGACCAACGGAGGAAGAGTTGGAGAATTTTGGGACACCGGATTTCACGATATACAATGCTGGACAGTTCCCTTGCAACCGTTTTACACATTACATGACATCTTCAACTAGCATAGATGTGAATTTGGCAAGAAAAGAAATGGTGATCCTTGGAACACAGTATGCAGGAGAGATGAAGAAAGGATTGTTCAGTTTGATGCATTATCTCATGCCCAAACGACATATTCTCTCCTTGCATTCTGGTTGTAATATGGGAAAAGATGGTGATGTTGCACTCTTCTTTGGGTTATCAGGTACTGGAAAGACGACTTTATCGACAGATCACAACCGTTACTTGATTGGAGACGATGAACATTGTTGGGGCAACGATGGTGTCTCAAACATTGAAGGAGGTTGTTATGCTAAGTGCATTGATCTTTCCCGAGAGAAAGAGCCTGATATCTGGAACGCTATCAAGTTTGGAACTGTGTTGGAGAACGTAGTATTCGATGAGCATACTAGAGAGGTTGACTATTCTGACAAATCAGTAACAGAGAACACTCGAGCGGCATACCCAATCGAGTACATACCTAACGCTCTGATACCATGCGTGGGGCCACATCCGAAGAACGTTATATTATTGGCTTGTGATGCATTTGGAGTGCTTCCTCCTGTGAGCAAGCTCAGTTTGGCTCAAACCATGTATCATTTCATCAGTGGATATACCGCATTGGTGGCTGGGACAGAAGATGGTATAAAGGAGCCACAAGCGACATTCTCGGCTTGCTTTGGAGCGGCTTTCATAATGTTACATCCCACAAAGTACGCCGCTATGCTCGCTCAAAAGATGCAGAACCACGGCGCTACCGGCTGGCTCGTTAACACTGGCTGGTCAAGCGGCCGATATGGCTACGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAACTTCTCAATGCGGATTATTATAAAACCGAAGTCTTTGGTCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCTGAGATTTTGGACCCAATGAACACGTGGTCGGATAAGGATGCGTATAATGAAACGTTGCTGAAATTGGCGAGTCTCTTCAAGAACAATTTTGAGACATTTACCAGTTACAAGATTGGTGAAGACAATAGCTTGACAGAGGAAATCCTTGCTGCTGGTCCGATATTTTAA

Protein sequence

MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSAPTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF
Homology
BLAST of PI0014163 vs. ExPASy Swiss-Prot
Match: Q9T074 (Phosphoenolpyruvate carboxykinase (ATP) 1 OS=Arabidopsis thaliana OX=3702 GN=PCK1 PE=1 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 533/647 (82.38%), Postives = 584/647 (90.26%), Query Frame = 0

Query: 15  KIQT-STKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIG-SAPTAFSLMAEEDR 74
           KI T + K+G+GVCHDDS   + A TIDELHSLQKKRS P+TPI  +A  AF+ ++EE+R
Sbjct: 25  KITTGAAKRGSGVCHDDSGPTVNATTIDELHSLQKKRSAPTTPINQNAAAAFAAVSEEER 84

Query: 75  QRQQLQSISASLASLTRETGPKVVKGDPARKETSTNV----AHVVHHQL--APTISISDS 134
           Q+ QLQSISASLASLTRE+GPKVV+GDPA K+T  +     AH  HH +    T ++SDS
Sbjct: 85  QKIQLQSISASLASLTRESGPKVVRGDPAEKKTDGSTTPAYAHGQHHSIFSPATGAVSDS 144

Query: 135 SMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTER 194
           S+KFTHVLYNLSPAELYEQAI+YEKGSF TS GALATLSGAKTGR+PRDKRVV+D  TE 
Sbjct: 145 SLKFTHVLYNLSPAELYEQAIKYEKGSFITSNGALATLSGAKTGRAPRDKRVVRDATTED 204

Query: 195 ELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAY 254
           ELWWGKGSPNIEMDEHTFM+NRERAVDYLNSL+KVFVNDQ+LNWDP N+IKVRIVSARAY
Sbjct: 205 ELWWGKGSPNIEMDEHTFMVNRERAVDYLNSLEKVFVNDQYLNWDPENRIKVRIVSARAY 264

Query: 255 HSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVIL 314
           HSLFMHNMCIRPT+EELE+FGTPDFTIYNAGQFPCNR+THYMTSSTS+D+NLAR+EMVIL
Sbjct: 265 HSLFMHNMCIRPTQEELESFGTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVIL 324

Query: 315 GTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR 374
           GTQYAGEMKKGLFS+MHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR
Sbjct: 325 GTQYAGEMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR 384

Query: 375 YLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 434
           YLIGDDEHCW   GVSNIEGGCYAKC+DLSREKEPDIWNAIKFGTVLENVVFDEHTREVD
Sbjct: 385 YLIGDDEHCWTETGVSNIEGGCYAKCVDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 444

Query: 435 YSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHF 494
           YSDKSVTENTRAAYPIE+IPNA IPCVGPHP NVILLACDAFGVLPPVSKL+LAQTMYHF
Sbjct: 445 YSDKSVTENTRAAYPIEFIPNAKIPCVGPHPTNVILLACDAFGVLPPVSKLNLAQTMYHF 504

Query: 495 ISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGW 554
           ISGYTALVAGTEDGIKEP ATFSACFGAAFIMLHPTKYAAMLA+KM++ GATGWLVNTGW
Sbjct: 505 ISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMKSQGATGWLVNTGW 564

Query: 555 SSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNT 614
           S G YG G+RIKL+YTRKIIDAIHSG LL A+Y KTE+FG EIPT IEG+PSEILDP+N+
Sbjct: 565 SGGSYGVGNRIKLAYTRKIIDAIHSGSLLKANYKKTEIFGFEIPTEIEGIPSEILDPVNS 624

Query: 615 WSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           WSDK A+ +TL+KL  LFK NFE F ++KIG D  LTEEILAAGPIF
Sbjct: 625 WSDKKAHKDTLVKLGGLFKKNFEVFANHKIGVDGKLTEEILAAGPIF 671

BLAST of PI0014163 vs. ExPASy Swiss-Prot
Match: P42066 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=PCK PE=2 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 5.9e-312
Identity = 515/627 (82.14%), Postives = 571/627 (91.07%), Query Frame = 0

Query: 26  VCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSAPTAFSLMAEEDRQRQQLQSISASLA 85
           +CHDDS++P++A+T++ LHSLQKKRSTP+TP+  +   FS ++E +RQ+QQL SISASLA
Sbjct: 42  ICHDDSTTPMRARTLEHLHSLQKKRSTPTTPLTDSQGVFSPVSEAERQKQQLISISASLA 101

Query: 86  SLTRETGPKVVKGDPARKETSTNVAHVVH-HQLAPTISISDSSMKFTHVLYNLSPAELYE 145
           SLTRETGPK+VKGDP +K+ +   + + H H   P +++SDS++K TH+LYN SPAELYE
Sbjct: 102 SLTRETGPKLVKGDPEKKKEAHKASVLDHLHFGEPILNLSDSALKSTHILYNPSPAELYE 161

Query: 146 QAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTERELWWGKGSPNIEMDEHTF 205
           QAI+YEKGSF TSTGALATLSGAKTGRSP DKRVVKD+ TE+ELWWGKGSPNIEMDEHTF
Sbjct: 162 QAIKYEKGSFITSTGALATLSGAKTGRSPIDKRVVKDDTTEKELWWGKGSPNIEMDEHTF 221

Query: 206 MINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAYHSLFMHNMCIRPTEEELE 265
           +INRERAVDYLNSLDKVFVNDQFLNWDP N+IKVRIVSARAYHSLFMHNMCIRPT  ELE
Sbjct: 222 LINRERAVDYLNSLDKVFVNDQFLNWDPENRIKVRIVSARAYHSLFMHNMCIRPTAGELE 281

Query: 266 NFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVILGTQYAGEMKKGLFSLMHY 325
           +FGTPDFTIY+AGQFPCNR+THYMTSSTSID+NL RKEMVILGTQYAGEMKKGLFSLMHY
Sbjct: 282 DFGTPDFTIYHAGQFPCNRYTHYMTSSTSIDMNLDRKEMVILGTQYAGEMKKGLFSLMHY 341

Query: 326 LMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGNDGVSNI 385
           LMP R ILSLHSGCNMGK+GDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW ++GVSNI
Sbjct: 342 LMPMRQILSLHSGCNMGKNGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSDNGVSNI 401

Query: 386 EGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAYPIEY 445
           EGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYS+KSVTENTRAAYPIEY
Sbjct: 402 EGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSEKSVTENTRAAYPIEY 461

Query: 446 IPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEDGIKEP 505
           IPNA IPCVGPHPKNVILLACDAFGVLPPVSKLSL QTMYHFISGYTALVAGTE+G+KEP
Sbjct: 462 IPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLSLPQTMYHFISGYTALVAGTEEGVKEP 521

Query: 506 QATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGWSSGRYGYGSRIKLSYTRK 565
           QATFSACFGAAFIMLHP++YAAMLA+KM+ HGATGWLVNTGWS G YG G+RIKL+YTRK
Sbjct: 522 QATFSACFGAAFIMLHPSRYAAMLAEKMKKHGATGWLVNTGWSGGSYGSGNRIKLAYTRK 581

Query: 566 IIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNTWSDKDAYNETLLKLASLF 625
           IIDAIHSG LL A+Y KT VFGLEIP AIEGVPS ILDP+NTWSDKD Y+ETLLKL  LF
Sbjct: 582 IIDAIHSGALLEANYSKTRVFGLEIPDAIEGVPSHILDPINTWSDKDGYHETLLKLGGLF 641

Query: 626 KNNFETFTSYKIGEDNSLTEEILAAGP 652
           K N+E   +Y++  D+ L EEILAAGP
Sbjct: 642 KKNYEGIHTYQVERDSELAEEILAAGP 668

BLAST of PI0014163 vs. ExPASy Swiss-Prot
Match: Q9SLZ0 (Phosphoenolpyruvate carboxykinase (ATP) OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 9.9e-312
Identity = 538/664 (81.02%), Postives = 584/664 (87.95%), Query Frame = 0

Query: 10  NGFAKKIQTS--TKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSAPTA---- 69
           NG A +I+T+   KQ NGV +DDSS+P++AQTIDELHSLQ+KRS P+TP  SAPT     
Sbjct: 5   NGLA-RIETTGKKKQDNGVWYDDSSAPVRAQTIDELHSLQRKRSAPTTPNRSAPTTPIKG 64

Query: 70  -----FSL-MAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARK-------ETSTNVA 129
                F++ ++EE+R  QQ+QSISASLASLTRETGPKVVKGDPA K         ST  A
Sbjct: 65  GAHSPFAVAISEEERHTQQMQSISASLASLTRETGPKVVKGDPAAKGEAAAQGAPSTPRA 124

Query: 130 HVVH-HQLAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKT 189
           H  H H  AP I++SDSS+KFTHVL NLSPAELYEQAI+YEKGSF TSTGALATLSGAKT
Sbjct: 125 HQQHRHPAAPAIAVSDSSLKFTHVLNNLSPAELYEQAIKYEKGSFITSTGALATLSGAKT 184

Query: 190 GRSPRDKRVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLN 249
           GRSPRDKRVVKDE T ++LWWGKGSPNIEMDE TF+INRERAVDYLNSL KV    Q LN
Sbjct: 185 GRSPRDKRVVKDEVTAQDLWWGKGSPNIEMDEKTFLINRERAVDYLNSL-KVVRQRQLLN 244

Query: 250 WDPTNKIKVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMT 309
           WDP N+IKVRI+SARAYHSLFMHNMCIRPT+EELE+FGTPDFTIYNAGQFPCNR+THYMT
Sbjct: 245 WDPENRIKVRIISARAYHSLFMHNMCIRPTDEELEDFGTPDFTIYNAGQFPCNRYTHYMT 304

Query: 310 SSTSIDVNLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALF 369
           SSTSID+NLAR+EMVI+GTQYAGEMKKGLF +MHYLMPKR ILSLHSGCNMGKDGDVALF
Sbjct: 305 SSTSIDLNLARREMVIMGTQYAGEMKKGLFGVMHYLMPKRGILSLHSGCNMGKDGDVALF 364

Query: 370 FGLSGTGKTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKF 429
           FGLSGTGKTTLSTDHN  LIGDDEHCW ++GVSNIEGGCYAKCIDL++EKEPDIWNAIKF
Sbjct: 365 FGLSGTGKTTLSTDHNGLLIGDDEHCWSDNGVSNIEGGCYAKCIDLAQEKEPDIWNAIKF 424

Query: 430 GTVLENVVFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFG 489
           GTVLENVVFDEHTREVDY+D SVTENTRAAYPIEYIP A IPCVGPHPKNVILLACDAFG
Sbjct: 425 GTVLENVVFDEHTREVDYADYSVTENTRAAYPIEYIPIAKIPCVGPHPKNVILLACDAFG 484

Query: 490 VLPPVSKLSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA 549
           VLPPVSKL LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA
Sbjct: 485 VLPPVSKLILAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA 544

Query: 550 QKMQNHGATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEI 609
           +KMQ +GATGWLVNTGWS GRYG G RI+L YTRKIIDAIHSGELL A+Y KTEVFGLEI
Sbjct: 545 EKMQKYGATGWLVNTGWSGGRYGVGKRIRLPYTRKIIDAIHSGELLTANYQKTEVFGLEI 604

Query: 610 PTAIEGVPSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAA 654
           PT I GVPSEILDP+ TW+DK AY E LL+L  LFKNNFE F SYKIG+D+SLT+EILAA
Sbjct: 605 PTEINGVPSEILDPIYTWTDKAAYKENLLRLGGLFKNNFEVFASYKIGDDSSLTDEILAA 664

BLAST of PI0014163 vs. ExPASy Swiss-Prot
Match: B5X574 (Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Arabidopsis thaliana OX=3702 GN=PCK2 PE=1 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 7.4e-307
Identity = 512/633 (80.88%), Postives = 559/633 (88.31%), Query Frame = 0

Query: 26  VCHDDSSSPIKAQTIDELHSLQKKRSTPSTPI----GSAPTAFSLMAEEDRQRQQLQSIS 85
           VC DD +  +  QTIDELHSLQKKRS P+TP+     S  +  S           LQS+S
Sbjct: 41  VCQDDIAPRVNFQTIDELHSLQKKRSAPTTPLRDGSASGVSGTSGPTTPVSSETMLQSVS 100

Query: 86  ASLASLTRETGPKVVKGDPARKETSTNVAHV-VHHQLAPTISISDSSMKFTHVLYNLSPA 145
           ASLASLTRETGPK+++GDP    ++  VAHV V     P   +SDS +KFTH+L+NLSPA
Sbjct: 101 ASLASLTRETGPKLIRGDPT---SAAKVAHVPVTPTSLPAADVSDSGLKFTHILHNLSPA 160

Query: 146 ELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTERELWWGKGSPNIEMD 205
           ELYEQAI++EKGSF TSTGALATLSGAKTGRSP+DKRVVKD+ TE ELWWGKGSPNIEMD
Sbjct: 161 ELYEQAIKFEKGSFVTSTGALATLSGAKTGRSPKDKRVVKDDTTEAELWWGKGSPNIEMD 220

Query: 206 EHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAYHSLFMHNMCIRPTE 265
           E TF++NRERAVDYLNSLDKVFVNDQ+LNWDP NKIKVRIVSARAYHSLFMHNMCIRPT 
Sbjct: 221 EKTFLVNRERAVDYLNSLDKVFVNDQYLNWDPENKIKVRIVSARAYHSLFMHNMCIRPTP 280

Query: 266 EELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVILGTQYAGEMKKGLFS 325
           EELENFGTPDFTIYNAG+FPCNRFTHYMTSSTS+D+NL R+EMVILGTQYAGEMKKGLF 
Sbjct: 281 EELENFGTPDFTIYNAGKFPCNRFTHYMTSSTSVDINLGRREMVILGTQYAGEMKKGLFG 340

Query: 326 LMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGNDG 385
           +MHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW   G
Sbjct: 341 VMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSEAG 400

Query: 386 VSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAY 445
           VSNIEGGCYAKCIDL+R+KEPDIWNAIKFGTVLENVVFDEHTREVDY+DKSVTENTRAAY
Sbjct: 401 VSNIEGGCYAKCIDLARDKEPDIWNAIKFGTVLENVVFDEHTREVDYTDKSVTENTRAAY 460

Query: 446 PIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEDG 505
           PIEYIPN+ IPCVGPHPKNVILLACDAFGVLPP+SKL+LAQTMYHFISGYTALVAGTE+G
Sbjct: 461 PIEYIPNSKIPCVGPHPKNVILLACDAFGVLPPISKLNLAQTMYHFISGYTALVAGTEEG 520

Query: 506 IKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGWSSGRYGYGSRIKLS 565
           +KEP+ATFSACFGAAFIMLHPTKYAAMLA+KMQ  GATGWLVNTGWS G YG GSRIKL+
Sbjct: 521 VKEPRATFSACFGAAFIMLHPTKYAAMLAEKMQAQGATGWLVNTGWSGGSYGTGSRIKLA 580

Query: 566 YTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNTWSDKDAYNETLLKL 625
           YTRKIIDAIHSG LLNA Y KT++FGLEIP  +EGVPSEIL+P+N W DK AY +TLLKL
Sbjct: 581 YTRKIIDAIHSGSLLNASYRKTDIFGLEIPNEVEGVPSEILEPINAWPDKMAYEDTLLKL 640

Query: 626 ASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           A LFK+NFETFTS+KIG+D  LTEEILAAGP F
Sbjct: 641 AGLFKSNFETFTSHKIGDDGKLTEEILAAGPNF 670

BLAST of PI0014163 vs. ExPASy Swiss-Prot
Match: Q9XFA2 (Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Urochloa panicoides OX=37563 GN=PCK2 PE=2 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 3.2e-294
Identity = 500/630 (79.37%), Postives = 553/630 (87.78%), Query Frame = 0

Query: 28  HD-DSSSPIKAQTIDELHSLQKKRSTPSTPIGSAPTAFSLMAEEDRQRQQLQSISASLAS 87
           HD DS++P+ AQTI+ELHSLQ+K +T +T  G++P               LQSISASLAS
Sbjct: 14  HDSDSAAPVNAQTIEELHSLQRKAAT-TTKDGASP---------------LQSISASLAS 73

Query: 88  LTRETGPKVVKGDPARKETSTNVAHVVHHQ---LAPTISISDSSMKFTHVLYNLSPAELY 147
           L RE GP +VKGDP   + +  V  + H Q    A TI+ SDSS+KFTHVLYNLSPAELY
Sbjct: 74  LAREYGPNLVKGDPEATKGAPPVP-IKHQQPSAAAATIAASDSSLKFTHVLYNLSPAELY 133

Query: 148 EQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTERELWWGKGSPNIEMDEHT 207
           EQA   +K SF TSTGALATLSGAKTGRSPRDKRVVKDE T +ELWWGKGSPNIEMDE  
Sbjct: 134 EQAFGQKKSSFITSTGALATLSGAKTGRSPRDKRVVKDETTSQELWWGKGSPNIEMDERQ 193

Query: 208 FMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAYHSLFMHNMCIRPTEEEL 267
           F+INRERA+DYLNSLDKV+VNDQFLNWD  N+IKVRI+++RAYH+LFMHNMCIRPTEEEL
Sbjct: 194 FVINRERALDYLNSLDKVYVNDQFLNWDSENRIKVRIITSRAYHALFMHNMCIRPTEEEL 253

Query: 268 ENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVILGTQYAGEMKKGLFSLMH 327
           E+FGTPDFTIYNAG+FP NR+ +YMTSSTSI+++LAR+EMVILGTQYAGEMKKGLF +MH
Sbjct: 254 ESFGTPDFTIYNAGEFPANRYANYMTSSTSINISLARREMVILGTQYAGEMKKGLFGVMH 313

Query: 328 YLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGNDGVSN 387
           YLMPKR ILSLHSGCNMGK+GDVALFFGLSGTGKTTLSTDHNR LIGDDEHCW ++GVSN
Sbjct: 314 YLMPKRGILSLHSGCNMGKEGDVALFFGLSGTGKTTLSTDHNRLLIGDDEHCWSDNGVSN 373

Query: 388 IEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAYPIE 447
           IEGGCYAKCIDLS+EKEPDIWNAIKFGTVLENVVF+E TREVDY+DKS+TENTRAAYPIE
Sbjct: 374 IEGGCYAKCIDLSQEKEPDIWNAIKFGTVLENVVFNERTREVDYADKSITENTRAAYPIE 433

Query: 448 YIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEDGIKE 507
           +IPNA IPCVGPHPKNVILLACDA+GVLPPVSKL+LAQTMYHFISGYTA+VAGTEDG+KE
Sbjct: 434 FIPNAKIPCVGPHPKNVILLACDAYGVLPPVSKLNLAQTMYHFISGYTAIVAGTEDGVKE 493

Query: 508 PQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGWSSGRYGYGSRIKLSYTR 567
           P ATFSACFGAAFIM HPTKYAAMLA+KMQ +GATGWLVNTGWS GRYG G+RIKL YTR
Sbjct: 494 PTATFSACFGAAFIMYHPTKYAAMLAEKMQKYGATGWLVNTGWSGGRYGVGNRIKLPYTR 553

Query: 568 KIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNTWSDKDAYNETLLKLASL 627
           KIIDAIHSGELLNA Y KTEVFGLEIPTAI GVPSEIL P+NTW+DK AY ETLLKLA L
Sbjct: 554 KIIDAIHSGELLNASYKKTEVFGLEIPTAINGVPSEILGPVNTWTDKAAYKETLLKLAGL 613

Query: 628 FKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           FKNNFE F SYKIG +NSLTE+ILAA P F
Sbjct: 614 FKNNFEVFASYKIGNNNSLTEQILAAAPNF 626

BLAST of PI0014163 vs. ExPASy TrEMBL
Match: A0A1S3B0T3 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo OX=3656 GN=LOC103484904 PE=3 SV=1)

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 620/654 (94.80%), Postives = 636/654 (97.25%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MADS+IS  NGFAKKI TSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTP+TPIGSA
Sbjct: 1   MADSIIS--NGFAKKINTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPTTPIGSA 60

Query: 61  P-TAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAP 120
           P +AFSL+ EEDRQRQQLQSISA+LASLTRETGPKVVKGDPARKETST VAHV HHQ AP
Sbjct: 61  PSSAFSLITEEDRQRQQLQSISATLASLTRETGPKVVKGDPARKETSTKVAHVAHHQYAP 120

Query: 121 TISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVV 180
            I+ISDSS+KFTHVLYNLSPAELYEQAIRYEKGSF TS GALATLSGAKTGRSP+DKRVV
Sbjct: 121 AINISDSSLKFTHVLYNLSPAELYEQAIRYEKGSFLTSVGALATLSGAKTGRSPKDKRVV 180

Query: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVR 240
           KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKIKVR
Sbjct: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIKVR 240

Query: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300
           IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA
Sbjct: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300

Query: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360
           RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT
Sbjct: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360

Query: 361 LSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420
           LSTD NRYLIGDDEHCW N+GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD
Sbjct: 361 LSTDQNRYLIGDDEHCWSNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420

Query: 421 EHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480
           EHTREVDYSD SVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL
Sbjct: 421 EHTREVDYSDNSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480

Query: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATG 540
           AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KMQNHGATG
Sbjct: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMQNHGATG 540

Query: 541 WLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSE 600
           WLVNTGWS GRYGYGSRIKLSYTRKIIDAIHSGELLNA+Y KT+VFGLEIPTAIEG+PSE
Sbjct: 541 WLVNTGWSGGRYGYGSRIKLSYTRKIIDAIHSGELLNANYCKTKVFGLEIPTAIEGIPSE 600

Query: 601 ILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           ILDPMNTW DKDAY+ETLLKLA+LFKNNFETFT+YKIGEDNSLTEEILAAGPIF
Sbjct: 601 ILDPMNTWPDKDAYDETLLKLANLFKNNFETFTNYKIGEDNSLTEEILAAGPIF 652

BLAST of PI0014163 vs. ExPASy TrEMBL
Match: A0A0A0KJU6 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=Csa_6G497180 PE=3 SV=1)

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 609/653 (93.26%), Postives = 632/653 (96.78%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MADS+IS  NGF+KKI TS KQGNGVC D SSSPIKAQTIDELHSLQKKRSTPSTP GS 
Sbjct: 1   MADSIIS--NGFSKKINTSMKQGNGVCQDGSSSPIKAQTIDELHSLQKKRSTPSTPSGSV 60

Query: 61  PTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAPT 120
            +AFS++ EEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETST VAHVVHHQL P 
Sbjct: 61  SSAFSVITEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTKVAHVVHHQLGPN 120

Query: 121 ISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVK 180
           ISISDSSMKFTHVLYNLSP ELYEQAIRYEKGSF TSTGALATLSGAKTGRSPRDKRVVK
Sbjct: 121 ISISDSSMKFTHVLYNLSPGELYEQAIRYEKGSFLTSTGALATLSGAKTGRSPRDKRVVK 180

Query: 181 DENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRI 240
           DENTE+ELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKI+VRI
Sbjct: 181 DENTEKELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIRVRI 240

Query: 241 VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLAR 300
           VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYN+GQFPCNRFTHYMTSSTSIDV+LAR
Sbjct: 241 VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNSGQFPCNRFTHYMTSSTSIDVSLAR 300

Query: 301 KEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTL 360
           KEMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTL
Sbjct: 301 KEMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTL 360

Query: 361 STDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 420
           STDHNR+LIGDDEHCWGN+GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE
Sbjct: 361 STDHNRFLIGDDEHCWGNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 420

Query: 421 HTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLA 480
           HTREVD++DKSVTENTRAAYPIEYIPNALIPCVGPHPKN+ILLACDAFGVLPPVSKL+LA
Sbjct: 421 HTREVDFTDKSVTENTRAAYPIEYIPNALIPCVGPHPKNIILLACDAFGVLPPVSKLTLA 480

Query: 481 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGW 540
           QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KM+ HGATGW
Sbjct: 481 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMKTHGATGW 540

Query: 541 LVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEI 600
           LVNTGWS GRYGYGSRIKLSYTRKIIDAIHSGELLNADY+KT++FGLEIP AIEG+PSEI
Sbjct: 541 LVNTGWSGGRYGYGSRIKLSYTRKIIDAIHSGELLNADYHKTKIFGLEIPNAIEGIPSEI 600

Query: 601 LDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           LDP NTWSDKDAY+ETLLKLASLFKNNFETFTSY+IGEDNSLTEEILAAGPIF
Sbjct: 601 LDPTNTWSDKDAYDETLLKLASLFKNNFETFTSYRIGEDNSLTEEILAAGPIF 651

BLAST of PI0014163 vs. ExPASy TrEMBL
Match: A0A6J1GEY4 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC111453577 PE=3 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 594/657 (90.41%), Postives = 627/657 (95.43%), Query Frame = 0

Query: 1   MADSVISNGNGFAK----KIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTP 60
           MADS + NGNG A     ++QTS K GNGVCHD SSSPIKAQTIDELHSLQKKRSTP+TP
Sbjct: 1   MADSKMKNGNGVANGSSTQVQTSPKHGNGVCHDGSSSPIKAQTIDELHSLQKKRSTPTTP 60

Query: 61  IGSAPTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQ 120
            GS+P+AFS+++EEDRQRQQLQSISASLASLTRETGPKVVKGDPARK  ++ +AHV HHQ
Sbjct: 61  TGSSPSAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQ 120

Query: 121 LAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDK 180
            APTI+ISDSS+KFTHVLYNLSPAELYEQAIRYE+GSFFTSTGALATLSGAKTGRSPRDK
Sbjct: 121 FAPTINISDSSLKFTHVLYNLSPAELYEQAIRYERGSFFTSTGALATLSGAKTGRSPRDK 180

Query: 181 RVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKI 240
           R+VKD NTE+ELWWGKGSPNIEMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP NK+
Sbjct: 181 RIVKDANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKL 240

Query: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDV 300
           KVRIVSARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSID+
Sbjct: 241 KVRIVSARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDM 300

Query: 301 NLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTG 360
           NLAR+EMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTG
Sbjct: 301 NLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTG 360

Query: 361 KTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420
           KTTLSTDHNRYLIGDDEHCWG++GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV
Sbjct: 361 KTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420

Query: 421 VFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSK 480
           VFDEHTREVDYSD SVTENTRAAYPIEYIPNA IPCVGPHPKNVILLACDAFGVLPPVSK
Sbjct: 421 VFDEHTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSK 480

Query: 481 LSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHG 540
           L+LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQ HG
Sbjct: 481 LNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHG 540

Query: 541 ATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGV 600
           ATGWLVNTGWS GRYG GSRIKLSYTRKIIDAIHSGELL A+Y KT+VFGLEIPTAIEGV
Sbjct: 541 ATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGV 600

Query: 601 PSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           PSEILDP++TWS+KDAYNETLLKLA+LFKNNFETFT+YKIGEDN+L E+ILAAGPIF
Sbjct: 601 PSEILDPISTWSNKDAYNETLLKLATLFKNNFETFTNYKIGEDNTLAEQILAAGPIF 657

BLAST of PI0014163 vs. ExPASy TrEMBL
Match: A0A6J1INJ3 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita maxima OX=3661 GN=LOC111478035 PE=3 SV=1)

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 593/657 (90.26%), Postives = 625/657 (95.13%), Query Frame = 0

Query: 1   MADSVISNGNGFAK----KIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTP 60
           MADS I NGNG A     ++QTS K GNG+CHD SSSPIKAQTIDELHSLQKKRSTP+TP
Sbjct: 1   MADSKIKNGNGVANGSSTQVQTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTP 60

Query: 61  IGSAPTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQ 120
            GS+P+AFS ++EEDRQRQQLQSISASLASLTRETGPKVVKGDPARK  ++ +AHV HHQ
Sbjct: 61  TGSSPSAFSALSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQ 120

Query: 121 LAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDK 180
            APTI+ISDSS+KFTHVLYNLSPAELYEQAIR E+GSFFTSTGALATLSGAKTGRSPRDK
Sbjct: 121 FAPTINISDSSLKFTHVLYNLSPAELYEQAIRCERGSFFTSTGALATLSGAKTGRSPRDK 180

Query: 181 RVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKI 240
           R+VKD NTE+ELWWGKGSPNIEMDEHTF INRERAVDYLNSLDKVFVNDQFLNWDP NK+
Sbjct: 181 RIVKDANTEKELWWGKGSPNIEMDEHTFTINRERAVDYLNSLDKVFVNDQFLNWDPQNKL 240

Query: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDV 300
           KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSID+
Sbjct: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDM 300

Query: 301 NLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTG 360
           NLAR+EMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTG
Sbjct: 301 NLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTG 360

Query: 361 KTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420
           KTTLSTDHNRYLIGDDEHCWG++GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV
Sbjct: 361 KTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420

Query: 421 VFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSK 480
           VFDE TREVDYSD SVTENTRAAYPIEYIPNA IPCVGPHPKNVILLACDAFGVLPPVSK
Sbjct: 421 VFDEQTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSK 480

Query: 481 LSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHG 540
           L+LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQ HG
Sbjct: 481 LNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHG 540

Query: 541 ATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGV 600
           ATGWLVNTGWS GRYG GSRIKLSYTRKIIDAIHSGELL A+Y KT+VFGLEIPTAIEGV
Sbjct: 541 ATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGV 600

Query: 601 PSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           PSEILDP++TWS++DAYNETLLKLA+LFKNNFETFT+YKIGEDN+L E+ILAAGPIF
Sbjct: 601 PSEILDPISTWSNRDAYNETLLKLATLFKNNFETFTNYKIGEDNTLAEQILAAGPIF 657

BLAST of PI0014163 vs. ExPASy TrEMBL
Match: A0A5A7SZ53 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003540 PE=3 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 595/654 (90.98%), Postives = 612/654 (93.58%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MADS+IS  NGFAKKI TSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTP+TPIGSA
Sbjct: 1   MADSIIS--NGFAKKINTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPTTPIGSA 60

Query: 61  P-TAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAP 120
           P +AFSL+ EEDRQRQQLQSISA+LASLTRETGPKVVKGDPARKETST VAHV HHQ AP
Sbjct: 61  PSSAFSLITEEDRQRQQLQSISATLASLTRETGPKVVKGDPARKETSTKVAHVAHHQYAP 120

Query: 121 TISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVV 180
            I+ISDSS+KFTHVLYNLSPAELYEQAIRYEKGSF TS GALATLSGAKTGRSP+DKRVV
Sbjct: 121 AINISDSSLKFTHVLYNLSPAELYEQAIRYEKGSFLTSVGALATLSGAKTGRSPKDKRVV 180

Query: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVR 240
           KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKIKVR
Sbjct: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIKVR 240

Query: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300
           IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA
Sbjct: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300

Query: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360
           RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT
Sbjct: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360

Query: 361 LSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420
           LSTD NRYLIGDDEHCW N+GVSNIEGG                        +LENVVFD
Sbjct: 361 LSTDQNRYLIGDDEHCWSNNGVSNIEGG------------------------LLENVVFD 420

Query: 421 EHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480
           EHTREVDYSD SVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL
Sbjct: 421 EHTREVDYSDNSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480

Query: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATG 540
           AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KMQNHGATG
Sbjct: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMQNHGATG 540

Query: 541 WLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSE 600
           WLVNTGWS GRYGYGSRIKLSYTRKIIDAIHSGELLNA+Y KT+VFGLEIPTAIEG+PSE
Sbjct: 541 WLVNTGWSGGRYGYGSRIKLSYTRKIIDAIHSGELLNANYCKTKVFGLEIPTAIEGIPSE 600

Query: 601 ILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           ILDPMNTW DKDAY+ETLLKLA+LFKNNFETFT+YKIGEDNSLTEEILAAGPIF
Sbjct: 601 ILDPMNTWPDKDAYDETLLKLANLFKNNFETFTNYKIGEDNSLTEEILAAGPIF 628

BLAST of PI0014163 vs. NCBI nr
Match: XP_008440491.1 (PREDICTED: phosphoenolpyruvate carboxykinase [ATP]-like [Cucumis melo])

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 620/654 (94.80%), Postives = 636/654 (97.25%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MADS+IS  NGFAKKI TSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTP+TPIGSA
Sbjct: 1   MADSIIS--NGFAKKINTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPTTPIGSA 60

Query: 61  P-TAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAP 120
           P +AFSL+ EEDRQRQQLQSISA+LASLTRETGPKVVKGDPARKETST VAHV HHQ AP
Sbjct: 61  PSSAFSLITEEDRQRQQLQSISATLASLTRETGPKVVKGDPARKETSTKVAHVAHHQYAP 120

Query: 121 TISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVV 180
            I+ISDSS+KFTHVLYNLSPAELYEQAIRYEKGSF TS GALATLSGAKTGRSP+DKRVV
Sbjct: 121 AINISDSSLKFTHVLYNLSPAELYEQAIRYEKGSFLTSVGALATLSGAKTGRSPKDKRVV 180

Query: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVR 240
           KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKIKVR
Sbjct: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIKVR 240

Query: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300
           IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA
Sbjct: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300

Query: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360
           RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT
Sbjct: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360

Query: 361 LSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420
           LSTD NRYLIGDDEHCW N+GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD
Sbjct: 361 LSTDQNRYLIGDDEHCWSNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420

Query: 421 EHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480
           EHTREVDYSD SVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL
Sbjct: 421 EHTREVDYSDNSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480

Query: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATG 540
           AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KMQNHGATG
Sbjct: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMQNHGATG 540

Query: 541 WLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSE 600
           WLVNTGWS GRYGYGSRIKLSYTRKIIDAIHSGELLNA+Y KT+VFGLEIPTAIEG+PSE
Sbjct: 541 WLVNTGWSGGRYGYGSRIKLSYTRKIIDAIHSGELLNANYCKTKVFGLEIPTAIEGIPSE 600

Query: 601 ILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           ILDPMNTW DKDAY+ETLLKLA+LFKNNFETFT+YKIGEDNSLTEEILAAGPIF
Sbjct: 601 ILDPMNTWPDKDAYDETLLKLANLFKNNFETFTNYKIGEDNSLTEEILAAGPIF 652

BLAST of PI0014163 vs. NCBI nr
Match: XP_004143427.1 (phosphoenolpyruvate carboxykinase (ATP) [Cucumis sativus] >KGN48667.1 hypothetical protein Csa_004095 [Cucumis sativus])

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 609/653 (93.26%), Postives = 632/653 (96.78%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MADS+IS  NGF+KKI TS KQGNGVC D SSSPIKAQTIDELHSLQKKRSTPSTP GS 
Sbjct: 1   MADSIIS--NGFSKKINTSMKQGNGVCQDGSSSPIKAQTIDELHSLQKKRSTPSTPSGSV 60

Query: 61  PTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQLAPT 120
            +AFS++ EEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETST VAHVVHHQL P 
Sbjct: 61  SSAFSVITEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTKVAHVVHHQLGPN 120

Query: 121 ISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVK 180
           ISISDSSMKFTHVLYNLSP ELYEQAIRYEKGSF TSTGALATLSGAKTGRSPRDKRVVK
Sbjct: 121 ISISDSSMKFTHVLYNLSPGELYEQAIRYEKGSFLTSTGALATLSGAKTGRSPRDKRVVK 180

Query: 181 DENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRI 240
           DENTE+ELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKI+VRI
Sbjct: 181 DENTEKELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIRVRI 240

Query: 241 VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLAR 300
           VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYN+GQFPCNRFTHYMTSSTSIDV+LAR
Sbjct: 241 VSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNSGQFPCNRFTHYMTSSTSIDVSLAR 300

Query: 301 KEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTL 360
           KEMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTL
Sbjct: 301 KEMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTL 360

Query: 361 STDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 420
           STDHNR+LIGDDEHCWGN+GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE
Sbjct: 361 STDHNRFLIGDDEHCWGNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 420

Query: 421 HTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLA 480
           HTREVD++DKSVTENTRAAYPIEYIPNALIPCVGPHPKN+ILLACDAFGVLPPVSKL+LA
Sbjct: 421 HTREVDFTDKSVTENTRAAYPIEYIPNALIPCVGPHPKNIILLACDAFGVLPPVSKLTLA 480

Query: 481 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGW 540
           QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KM+ HGATGW
Sbjct: 481 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMKTHGATGW 540

Query: 541 LVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEI 600
           LVNTGWS GRYGYGSRIKLSYTRKIIDAIHSGELLNADY+KT++FGLEIP AIEG+PSEI
Sbjct: 541 LVNTGWSGGRYGYGSRIKLSYTRKIIDAIHSGELLNADYHKTKIFGLEIPNAIEGIPSEI 600

Query: 601 LDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           LDP NTWSDKDAY+ETLLKLASLFKNNFETFTSY+IGEDNSLTEEILAAGPIF
Sbjct: 601 LDPTNTWSDKDAYDETLLKLASLFKNNFETFTSYRIGEDNSLTEEILAAGPIF 651

BLAST of PI0014163 vs. NCBI nr
Match: XP_038882835.1 (phosphoenolpyruvate carboxykinase (ATP) 1-like [Benincasa hispida])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 602/654 (92.05%), Postives = 627/654 (95.87%), Query Frame = 0

Query: 1   MADSVISNGNGFAKKIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIGSA 60
           MA S I+NG  F ++IQ  T+ GNG+C+DDS+SPIKAQTIDELH+LQKKRSTP TPIGSA
Sbjct: 1   MAGSNIANGKEFDREIQ--TEGGNGICYDDSTSPIKAQTIDELHNLQKKRSTPGTPIGSA 60

Query: 61  PTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTN-VAHVVHHQLAP 120
              FSL+AEEDRQRQQLQSISASLASL RETGPKVVKGDPARK T+TN VAHV+HHQLAP
Sbjct: 61  ADTFSLIAEEDRQRQQLQSISASLASLARETGPKVVKGDPARKSTTTNEVAHVLHHQLAP 120

Query: 121 TISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVV 180
           TI+ISDSS+KFTHVLYNLSPAELYEQAI+YEKGSFFTSTGALATLSGAKTGRSPRDKRVV
Sbjct: 121 TINISDSSLKFTHVLYNLSPAELYEQAIKYEKGSFFTSTGALATLSGAKTGRSPRDKRVV 180

Query: 181 KDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVR 240
           KDENTE+ELWWGKGSPNIEMD HTFMINRERAVDYLNSLDKVFVNDQFLNWDP NKIKVR
Sbjct: 181 KDENTEKELWWGKGSPNIEMDGHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIKVR 240

Query: 241 IVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLA 300
           IVSARAYHSLFMHNMCIRPTEEEL NFGTPDFTIYNAGQFPCNRFTHYMTSSTS+DVNL 
Sbjct: 241 IVSARAYHSLFMHNMCIRPTEEELVNFGTPDFTIYNAGQFPCNRFTHYMTSSTSVDVNLT 300

Query: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360
           RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT
Sbjct: 301 RKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTT 360

Query: 361 LSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420
           LSTD NRYLIGDDEHCW N+GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD
Sbjct: 361 LSTDQNRYLIGDDEHCWSNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFD 420

Query: 421 EHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSL 480
           EHTREVDYSD SVTENTRAAYPI+YIPNALIPCVGPHPKNVILLACDAFGVLPPVSKL+L
Sbjct: 421 EHTREVDYSDYSVTENTRAAYPIKYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLNL 480

Query: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATG 540
           AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KM+NHGATG
Sbjct: 481 AQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMKNHGATG 540

Query: 541 WLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSE 600
           WLVNTGWS G YG GSRIKLSYTRKIIDAIHSGELLNADY KT+VFGLEIPTAI+GVPSE
Sbjct: 541 WLVNTGWSGGCYGSGSRIKLSYTRKIIDAIHSGELLNADYRKTKVFGLEIPTAIKGVPSE 600

Query: 601 ILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           ILDP+NTWSDKDAYNETLLKLASLFKNNFETFT+YKIGEDNSLTEEILAAGP F
Sbjct: 601 ILDPVNTWSDKDAYNETLLKLASLFKNNFETFTNYKIGEDNSLTEEILAAGPNF 652

BLAST of PI0014163 vs. NCBI nr
Match: XP_022950481.1 (phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita moschata])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 594/657 (90.41%), Postives = 627/657 (95.43%), Query Frame = 0

Query: 1   MADSVISNGNGFAK----KIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTP 60
           MADS + NGNG A     ++QTS K GNGVCHD SSSPIKAQTIDELHSLQKKRSTP+TP
Sbjct: 1   MADSKMKNGNGVANGSSTQVQTSPKHGNGVCHDGSSSPIKAQTIDELHSLQKKRSTPTTP 60

Query: 61  IGSAPTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQ 120
            GS+P+AFS+++EEDRQRQQLQSISASLASLTRETGPKVVKGDPARK  ++ +AHV HHQ
Sbjct: 61  TGSSPSAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQ 120

Query: 121 LAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDK 180
            APTI+ISDSS+KFTHVLYNLSPAELYEQAIRYE+GSFFTSTGALATLSGAKTGRSPRDK
Sbjct: 121 FAPTINISDSSLKFTHVLYNLSPAELYEQAIRYERGSFFTSTGALATLSGAKTGRSPRDK 180

Query: 181 RVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKI 240
           R+VKD NTE+ELWWGKGSPNIEMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP NK+
Sbjct: 181 RIVKDANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKL 240

Query: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDV 300
           KVRIVSARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSID+
Sbjct: 241 KVRIVSARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDM 300

Query: 301 NLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTG 360
           NLAR+EMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTG
Sbjct: 301 NLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTG 360

Query: 361 KTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420
           KTTLSTDHNRYLIGDDEHCWG++GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV
Sbjct: 361 KTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420

Query: 421 VFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSK 480
           VFDEHTREVDYSD SVTENTRAAYPIEYIPNA IPCVGPHPKNVILLACDAFGVLPPVSK
Sbjct: 421 VFDEHTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSK 480

Query: 481 LSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHG 540
           L+LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQ HG
Sbjct: 481 LNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHG 540

Query: 541 ATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGV 600
           ATGWLVNTGWS GRYG GSRIKLSYTRKIIDAIHSGELL A+Y KT+VFGLEIPTAIEGV
Sbjct: 541 ATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGV 600

Query: 601 PSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           PSEILDP++TWS+KDAYNETLLKLA+LFKNNFETFT+YKIGEDN+L E+ILAAGPIF
Sbjct: 601 PSEILDPISTWSNKDAYNETLLKLATLFKNNFETFTNYKIGEDNTLAEQILAAGPIF 657

BLAST of PI0014163 vs. NCBI nr
Match: XP_023544791.1 (phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 592/657 (90.11%), Postives = 627/657 (95.43%), Query Frame = 0

Query: 1   MADSVISNGNGFAK----KIQTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTP 60
           MADS + NGNG A     ++ TS K GNG+CHD SSSPIKAQTIDELHSLQKKRSTP+TP
Sbjct: 1   MADSKMKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTP 60

Query: 61  IGSAPTAFSLMAEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTNVAHVVHHQ 120
            GS+P+AFS+++EEDRQRQQLQSISASLASLTRETGPKVVKGDPARK  ++ +AHV HHQ
Sbjct: 61  TGSSPSAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQ 120

Query: 121 LAPTISISDSSMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDK 180
            APTI+ISDSS+KFTHVLYNLSPAELYEQAIR+E+GSFFTSTGALATLSGAKTGRSPRDK
Sbjct: 121 FAPTINISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDK 180

Query: 181 RVVKDENTERELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKI 240
           R+VKD NTE+ELWWGKGSPNIEMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP NK+
Sbjct: 181 RIVKDANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKL 240

Query: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDV 300
           KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSID+
Sbjct: 241 KVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDM 300

Query: 301 NLARKEMVILGTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTG 360
           NLAR+EMVILGTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTG
Sbjct: 301 NLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTG 360

Query: 361 KTTLSTDHNRYLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420
           KTTLSTDHNRYLIGDDEHCWG++GVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV
Sbjct: 361 KTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENV 420

Query: 421 VFDEHTREVDYSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSK 480
           VFDEHTREVDYSD SVTENTRAAYPIEYIPNA IPCVGPHPKNVILLACDAFGVLPPVSK
Sbjct: 421 VFDEHTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSK 480

Query: 481 LSLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHG 540
           L+LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQ HG
Sbjct: 481 LNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHG 540

Query: 541 ATGWLVNTGWSSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGV 600
           ATGWLVNTGWS GRYG GSRIKLSYTRKIIDAIHSGELL A+Y KT+VFGLEIPTAIEGV
Sbjct: 541 ATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGV 600

Query: 601 PSEILDPMNTWSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           PSEILDP++TWS+KDAYNETLLKLA+LFKNNFETFT+YKIGEDN+L E+ILAAGPIF
Sbjct: 601 PSEILDPISTWSNKDAYNETLLKLATLFKNNFETFTNYKIGEDNTLAEQILAAGPIF 657

BLAST of PI0014163 vs. TAIR 10
Match: AT4G37870.1 (phosphoenolpyruvate carboxykinase 1 )

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 533/647 (82.38%), Postives = 584/647 (90.26%), Query Frame = 0

Query: 15  KIQT-STKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPSTPIG-SAPTAFSLMAEEDR 74
           KI T + K+G+GVCHDDS   + A TIDELHSLQKKRS P+TPI  +A  AF+ ++EE+R
Sbjct: 25  KITTGAAKRGSGVCHDDSGPTVNATTIDELHSLQKKRSAPTTPINQNAAAAFAAVSEEER 84

Query: 75  QRQQLQSISASLASLTRETGPKVVKGDPARKETSTNV----AHVVHHQL--APTISISDS 134
           Q+ QLQSISASLASLTRE+GPKVV+GDPA K+T  +     AH  HH +    T ++SDS
Sbjct: 85  QKIQLQSISASLASLTRESGPKVVRGDPAEKKTDGSTTPAYAHGQHHSIFSPATGAVSDS 144

Query: 135 SMKFTHVLYNLSPAELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTER 194
           S+KFTHVLYNLSPAELYEQAI+YEKGSF TS GALATLSGAKTGR+PRDKRVV+D  TE 
Sbjct: 145 SLKFTHVLYNLSPAELYEQAIKYEKGSFITSNGALATLSGAKTGRAPRDKRVVRDATTED 204

Query: 195 ELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAY 254
           ELWWGKGSPNIEMDEHTFM+NRERAVDYLNSL+KVFVNDQ+LNWDP N+IKVRIVSARAY
Sbjct: 205 ELWWGKGSPNIEMDEHTFMVNRERAVDYLNSLEKVFVNDQYLNWDPENRIKVRIVSARAY 264

Query: 255 HSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVIL 314
           HSLFMHNMCIRPT+EELE+FGTPDFTIYNAGQFPCNR+THYMTSSTS+D+NLAR+EMVIL
Sbjct: 265 HSLFMHNMCIRPTQEELESFGTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVIL 324

Query: 315 GTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR 374
           GTQYAGEMKKGLFS+MHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR
Sbjct: 325 GTQYAGEMKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR 384

Query: 375 YLIGDDEHCWGNDGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 434
           YLIGDDEHCW   GVSNIEGGCYAKC+DLSREKEPDIWNAIKFGTVLENVVFDEHTREVD
Sbjct: 385 YLIGDDEHCWTETGVSNIEGGCYAKCVDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 444

Query: 435 YSDKSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHF 494
           YSDKSVTENTRAAYPIE+IPNA IPCVGPHP NVILLACDAFGVLPPVSKL+LAQTMYHF
Sbjct: 445 YSDKSVTENTRAAYPIEFIPNAKIPCVGPHPTNVILLACDAFGVLPPVSKLNLAQTMYHF 504

Query: 495 ISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGW 554
           ISGYTALVAGTEDGIKEP ATFSACFGAAFIMLHPTKYAAMLA+KM++ GATGWLVNTGW
Sbjct: 505 ISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMKSQGATGWLVNTGW 564

Query: 555 SSGRYGYGSRIKLSYTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNT 614
           S G YG G+RIKL+YTRKIIDAIHSG LL A+Y KTE+FG EIPT IEG+PSEILDP+N+
Sbjct: 565 SGGSYGVGNRIKLAYTRKIIDAIHSGSLLKANYKKTEIFGFEIPTEIEGIPSEILDPVNS 624

Query: 615 WSDKDAYNETLLKLASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           WSDK A+ +TL+KL  LFK NFE F ++KIG D  LTEEILAAGPIF
Sbjct: 625 WSDKKAHKDTLVKLGGLFKKNFEVFANHKIGVDGKLTEEILAAGPIF 671

BLAST of PI0014163 vs. TAIR 10
Match: AT5G65690.1 (phosphoenolpyruvate carboxykinase 2 )

HSP 1 Score: 1053.9 bits (2724), Expect = 5.3e-308
Identity = 512/633 (80.88%), Postives = 559/633 (88.31%), Query Frame = 0

Query: 26  VCHDDSSSPIKAQTIDELHSLQKKRSTPSTPI----GSAPTAFSLMAEEDRQRQQLQSIS 85
           VC DD +  +  QTIDELHSLQKKRS P+TP+     S  +  S           LQS+S
Sbjct: 41  VCQDDIAPRVNFQTIDELHSLQKKRSAPTTPLRDGSASGVSGTSGPTTPVSSETMLQSVS 100

Query: 86  ASLASLTRETGPKVVKGDPARKETSTNVAHV-VHHQLAPTISISDSSMKFTHVLYNLSPA 145
           ASLASLTRETGPK+++GDP    ++  VAHV V     P   +SDS +KFTH+L+NLSPA
Sbjct: 101 ASLASLTRETGPKLIRGDPT---SAAKVAHVPVTPTSLPAADVSDSGLKFTHILHNLSPA 160

Query: 146 ELYEQAIRYEKGSFFTSTGALATLSGAKTGRSPRDKRVVKDENTERELWWGKGSPNIEMD 205
           ELYEQAI++EKGSF TSTGALATLSGAKTGRSP+DKRVVKD+ TE ELWWGKGSPNIEMD
Sbjct: 161 ELYEQAIKFEKGSFVTSTGALATLSGAKTGRSPKDKRVVKDDTTEAELWWGKGSPNIEMD 220

Query: 206 EHTFMINRERAVDYLNSLDKVFVNDQFLNWDPTNKIKVRIVSARAYHSLFMHNMCIRPTE 265
           E TF++NRERAVDYLNSLDKVFVNDQ+LNWDP NKIKVRIVSARAYHSLFMHNMCIRPT 
Sbjct: 221 EKTFLVNRERAVDYLNSLDKVFVNDQYLNWDPENKIKVRIVSARAYHSLFMHNMCIRPTP 280

Query: 266 EELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVILGTQYAGEMKKGLFS 325
           EELENFGTPDFTIYNAG+FPCNRFTHYMTSSTS+D+NL R+EMVILGTQYAGEMKKGLF 
Sbjct: 281 EELENFGTPDFTIYNAGKFPCNRFTHYMTSSTSVDINLGRREMVILGTQYAGEMKKGLFG 340

Query: 326 LMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGNDG 385
           +MHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW   G
Sbjct: 341 VMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSEAG 400

Query: 386 VSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVTENTRAAY 445
           VSNIEGGCYAKCIDL+R+KEPDIWNAIKFGTVLENVVFDEHTREVDY+DKSVTENTRAAY
Sbjct: 401 VSNIEGGCYAKCIDLARDKEPDIWNAIKFGTVLENVVFDEHTREVDYTDKSVTENTRAAY 460

Query: 446 PIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEDG 505
           PIEYIPN+ IPCVGPHPKNVILLACDAFGVLPP+SKL+LAQTMYHFISGYTALVAGTE+G
Sbjct: 461 PIEYIPNSKIPCVGPHPKNVILLACDAFGVLPPISKLNLAQTMYHFISGYTALVAGTEEG 520

Query: 506 IKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQNHGATGWLVNTGWSSGRYGYGSRIKLS 565
           +KEP+ATFSACFGAAFIMLHPTKYAAMLA+KMQ  GATGWLVNTGWS G YG GSRIKL+
Sbjct: 521 VKEPRATFSACFGAAFIMLHPTKYAAMLAEKMQAQGATGWLVNTGWSGGSYGTGSRIKLA 580

Query: 566 YTRKIIDAIHSGELLNADYYKTEVFGLEIPTAIEGVPSEILDPMNTWSDKDAYNETLLKL 625
           YTRKIIDAIHSG LLNA Y KT++FGLEIP  +EGVPSEIL+P+N W DK AY +TLLKL
Sbjct: 581 YTRKIIDAIHSGSLLNASYRKTDIFGLEIPNEVEGVPSEILEPINAWPDKMAYEDTLLKL 640

Query: 626 ASLFKNNFETFTSYKIGEDNSLTEEILAAGPIF 654
           A LFK+NFETFTS+KIG+D  LTEEILAAGP F
Sbjct: 641 AGLFKSNFETFTSHKIGDDGKLTEEILAAGPNF 670

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0740.0e+0082.38Phosphoenolpyruvate carboxykinase (ATP) 1 OS=Arabidopsis thaliana OX=3702 GN=PCK... [more]
P420665.9e-31282.14Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=PCK PE=2 S... [more]
Q9SLZ09.9e-31281.02Phosphoenolpyruvate carboxykinase (ATP) OS=Zea mays OX=4577 PE=2 SV=1[more]
B5X5747.4e-30780.88Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Arabidopsis thaliana OX=3702 GN=PCK... [more]
Q9XFA23.2e-29479.37Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Urochloa panicoides OX=37563 GN=PCK... [more]
Match NameE-valueIdentityDescription
A0A1S3B0T30.0e+0094.80Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo OX=3656 GN=LOC103484904 ... [more]
A0A0A0KJU60.0e+0093.26Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=Csa_6G4971... [more]
A0A6J1GEY40.0e+0090.41Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1INJ30.0e+0090.26Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A5A7SZ530.0e+0090.98Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_008440491.10.0e+0094.80PREDICTED: phosphoenolpyruvate carboxykinase [ATP]-like [Cucumis melo][more]
XP_004143427.10.0e+0093.26phosphoenolpyruvate carboxykinase (ATP) [Cucumis sativus] >KGN48667.1 hypothetic... [more]
XP_038882835.10.0e+0092.05phosphoenolpyruvate carboxykinase (ATP) 1-like [Benincasa hispida][more]
XP_022950481.10.0e+0090.41phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita moschata][more]
XP_023544791.10.0e+0090.11phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G37870.10.0e+0082.38phosphoenolpyruvate carboxykinase 1 [more]
AT5G65690.15.3e-30880.88phosphoenolpyruvate carboxykinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013035Phosphoenolpyruvate carboxykinase, C-terminalGENE3D3.90.228.20coord: 338..632
e-value: 1.2E-216
score: 722.3
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingPIRSFPIRSF006294PEP_crbxkincoord: 113..653
e-value: 7.1E-256
score: 847.8
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingTIGRFAMTIGR00224TIGR00224coord: 129..651
e-value: 1.8E-222
score: 737.8
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingPFAMPF01293PEPCK_ATPcoord: 132..599
e-value: 6.2E-211
score: 701.0
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingPANTHERPTHR30031PHOSPHOENOLPYRUVATE CARBOXYKINASE ATPcoord: 15..653
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingHAMAPMF_00453PEPCK_ATPcoord: 126..653
score: 48.172157
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingCDDcd00484PEPCK_ATPcoord: 131..650
e-value: 0.0
score: 986.72
IPR008210Phosphoenolpyruvate carboxykinase, N-terminalGENE3D3.40.449.10Phosphoenolpyruvate Carboxykinase, domain 1coord: 130..337
e-value: 1.2E-216
score: 722.3
IPR008210Phosphoenolpyruvate carboxykinase, N-terminalSUPERFAMILY68923PEP carboxykinase N-terminal domaincoord: 131..329
NoneNo IPR availableGENE3D2.170.8.10Phosphoenolpyruvate Carboxykinase, domain 2coord: 150..453
e-value: 1.2E-216
score: 722.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..107
NoneNo IPR availablePANTHERPTHR30031:SF10PHOSPHOENOLPYRUVATE CARBOXYKINASE [ATP] PROTEINcoord: 15..653
NoneNo IPR availableSUPERFAMILY53795PEP carboxykinase-likecoord: 331..652
IPR015994Phosphoenolpyruvate carboxykinase (ATP), conserved sitePROSITEPS00532PEPCK_ATPcoord: 368..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0014163.1PI0014163.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0016310 phosphorylation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
molecular_function GO:0004611 phosphoenolpyruvate carboxykinase activity
molecular_function GO:0017076 purine nucleotide binding