PI0013949 (gene) Melon (PI 482460) v1

Overview
NamePI0013949
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectate lyase
Locationchr12: 2842775 .. 2845247 (-)
RNA-Seq ExpressionPI0013949
SyntenyPI0013949
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTTTCCACTCACAAAATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTCCCTCTTCCTCTTCTCCATTTCTCTCTCCGCCGCTCTCTCCTCCGCCCCTGCCGCCGTTCCTAACCCAGAACTCGTCGTACAAGAAGTACACAGGTATAGAAAACACCAACATTCCATAAGCTCTGTTTTTTTTTTTTTTTCCTTGTTTTGTTCCTCTGTTTTGAAAATGTGCGTCTCATTTTGTGTTTACAGGAGCATAATCAATGCAACGAAGAGGAGGAATTTGGGGTATCTCTCATGTGGGACTGGAAATCCAATCGATGATTGCTGGCGGTGCGATTCCAATTGGGAGAAAAATCGTCAGAGATTAGCTGATTGTGGGATCGGTTTCGGCAAAAACGCCATCGGCGGTCGAGACGGCAAAATCTACGTCGTTACTGACGCCGGCGACTACGATCCCGTCAACCCCAAGCCAGGAACTCTCCGTTACGCCGTAATCCAAGACGAACCCCTTTGGATCATCTTCGCTCGCGACATGGTCATCCGATTGAAAGAGGAACTGATTATGAATTCTTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACAAACATTATAATCCATGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACCGATGTGAGAGATTCCCCTCGACATTTTGGATTCAGAACCATATCGGACGGTGACGGCGTTTCGATCTTCGGCGGAAGCCATGTTTGGGTTGATCATTGTTCTTTATCGAACTGCAATGATGGGTTAATCGACGCGATTCATGGGTCTACAGCGATTACGATTTCGAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTATACTCAGGACAAGAATATGCAAGTCACCATTGCTTTTAATCACTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGGTAAAAATAGGAATCGAACATTTAATTTCATATTCTCTGTGTTTTGGTTGATGAAATTGAAATTGAAATTGGAATTGGGGGATTTTGGTTTTGCAGATGCAGACATGGGTATTTTCATGTGGTGAACAATGACTATACACATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACCATCAACAGTCAAGGGAACAGATTTGTTGCTCCAAATGACAGATTCAGCAAAGAGGTAAGAACATAAACAATACAAAAAAAAAAAAAAAAATGATACTCTTTTTCTTTAGTTTGTTGGTTTGATATTTTTTAAAAATTGTTTGATGAGCTCTCAAACTTTTTGTTAAATTGCAAAGTAAGTCCCTATATTTTGAAGAAAACTAAACCTTGGTACTTATGGTTTATAATTATAATTTAGCAATTTTGATAAAATCTTCAATAAAAAGTTTTTATATGAGAATTTTATTAAACATAGGATAATAAGGAAAACAATAATTTTAACTTTTTGTTCAATTCTTCATAATTTTTTAAATATTACACTTGATTTAAATTTTTAAAAATCTTTATTTAAATATCAAATGAACACAATTGGACTCCAAGGGGTAGGAGTAGACATACAACCAGAAAATATCAATAAGTTGGCACCCACAACCAAAAAAAAAAGTGTACAAACCTAAGAAAATATGCAAATAAGAAAGAGTTGAAGGACTAAGGGCTAAATTTGTAATTTTCATTGTTTGACGTGATGGATAAAAAGTAGTATTATATTGTGGATTTGTGATCTTTATGGGATATTGGTGAATGGCAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGGTCAGAAGGAGATCTGATGTTAAACGGTGCGTTTTTCACATCGTCCGGTGCCGGAGCATCGTCCAGTTATGCAAGAGCATCGAGCTTAGGAGCGAGACCGTCGTCGCTGGTCGGTACAATTACAACCAATGCAGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGATTATTATTACTCCTACCCAAATTGAAATTTGAAAAGTCTAGTAGCTAGTTTGTAGTTGGTTGGTTGGTTGATTTTCTTAAAAGTTTAAAATTGTTGATTTTATGAAAATGGTAATCTGAGGGAAAATCAGTAGAAAATGGAGGGAAAGTGAAAGAGGAAAAAAAAAAAAAAAAGAAAGAAAGAAAAAGAGAGCTAAGAAAAGAGACTGAACCAATAGGGGTCAGTCAATCTGCTTCTATCATTATATCTTTTTTTAGCTTTTTTTTTTCTTTCTTTTTCTCCTTCCATTTTCTTTTATTTTTTATTTTTGGTTTTTGGGTTGTTTTTATAATTATTACAATGTACAACCTCCGCATGTAATTCTTGAAACTGTAGGGGCTTTCCTTTTAAGAACCCCTCAAATGGTTTCAAATCAAGTCAAATGCAGAAGAGTTGATATTTGATATACTAA

mRNA sequence

CATTTTTCCACTCACAAAATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTCCCTCTTCCTCTTCTCCATTTCTCTCTCCGCCGCTCTCTCCTCCGCCCCTGCCGCCGTTCCTAACCCAGAACTCGTCGTACAAGAAGTACACAGGAGCATAATCAATGCAACGAAGAGGAGGAATTTGGGGTATCTCTCATGTGGGACTGGAAATCCAATCGATGATTGCTGGCGGTGCGATTCCAATTGGGAGAAAAATCGTCAGAGATTAGCTGATTGTGGGATCGGTTTCGGCAAAAACGCCATCGGCGGTCGAGACGGCAAAATCTACGTCGTTACTGACGCCGGCGACTACGATCCCGTCAACCCCAAGCCAGGAACTCTCCGTTACGCCGTAATCCAAGACGAACCCCTTTGGATCATCTTCGCTCGCGACATGGTCATCCGATTGAAAGAGGAACTGATTATGAATTCTTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACAAACATTATAATCCATGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACCGATGTGAGAGATTCCCCTCGACATTTTGGATTCAGAACCATATCGGACGGTGACGGCGTTTCGATCTTCGGCGGAAGCCATGTTTGGGTTGATCATTGTTCTTTATCGAACTGCAATGATGGGTTAATCGACGCGATTCATGGGTCTACAGCGATTACGATTTCGAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTATACTCAGGACAAGAATATGCAAGTCACCATTGCTTTTAATCACTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGATGCAGACATGGGTATTTTCATGTGGTGAACAATGACTATACACATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACCATCAACAGTCAAGGGAACAGATTTGTTGCTCCAAATGACAGATTCAGCAAAGAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGGTCAGAAGGAGATCTGATGTTAAACGGTGCGTTTTTCACATCGTCCGGTGCCGGAGCATCGTCCAGTTATGCAAGAGCATCGAGCTTAGGAGCGAGACCGTCGTCGCTGGTCGGTACAATTACAACCAATGCAGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGATTATTATTACTCCTACCCAAATTGAAATTTGAAAAGTCTAGTAGCTAGTTTGTAGTTGGTTGGTTGGTTGATTTTCTTAAAAGTTTAAAATTGTTGATTTTATGAAAATGGTAATCTGAGGGAAAATCAGTAGAAAATGGAGGGAAAGTGAAAGAGGAAAAAAAAAAAAAAAAGAAAGAAAGAAAAAGAGAGCTAAGAAAAGAGACTGAACCAATAGGGGTCAGTCAATCTGCTTCTATCATTATATCTTTTTTTAGCTTTTTTTTTTCTTTCTTTTTCTCCTTCCATTTTCTTTTATTTTTTATTTTTGGTTTTTGGGTTGTTTTTATAATTATTACAATGTACAACCTCCGCATGTAATTCTTGAAACTGTAGGGGCTTTCCTTTTAAGAACCCCTCAAATGGTTTCAAATCAAGTCAAATGCAGAAGAGTTGATATTTGATATACTAA

Coding sequence (CDS)

ATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTCCCTCTTCCTCTTCTCCATTTCTCTCTCCGCCGCTCTCTCCTCCGCCCCTGCCGCCGTTCCTAACCCAGAACTCGTCGTACAAGAAGTACACAGGAGCATAATCAATGCAACGAAGAGGAGGAATTTGGGGTATCTCTCATGTGGGACTGGAAATCCAATCGATGATTGCTGGCGGTGCGATTCCAATTGGGAGAAAAATCGTCAGAGATTAGCTGATTGTGGGATCGGTTTCGGCAAAAACGCCATCGGCGGTCGAGACGGCAAAATCTACGTCGTTACTGACGCCGGCGACTACGATCCCGTCAACCCCAAGCCAGGAACTCTCCGTTACGCCGTAATCCAAGACGAACCCCTTTGGATCATCTTCGCTCGCGACATGGTCATCCGATTGAAAGAGGAACTGATTATGAATTCTTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACAAACATTATAATCCATGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACCGATGTGAGAGATTCCCCTCGACATTTTGGATTCAGAACCATATCGGACGGTGACGGCGTTTCGATCTTCGGCGGAAGCCATGTTTGGGTTGATCATTGTTCTTTATCGAACTGCAATGATGGGTTAATCGACGCGATTCATGGGTCTACAGCGATTACGATTTCGAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTATACTCAGGACAAGAATATGCAAGTCACCATTGCTTTTAATCACTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGATGCAGACATGGGTATTTTCATGTGGTGAACAATGACTATACACATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACCATCAACAGTCAAGGGAACAGATTTGTTGCTCCAAATGACAGATTCAGCAAAGAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGGTCAGAAGGAGATCTGATGTTAAACGGTGCGTTTTTCACATCGTCCGGTGCCGGAGCATCGTCCAGTTATGCAAGAGCATCGAGCTTAGGAGCGAGACCGTCGTCGCTGGTCGGTACAATTACAACCAATGCAGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGA

Protein sequence

MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Homology
BLAST of PI0013949 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 2.6e-196
Identity = 328/406 (80.79%), Postives = 361/406 (88.92%), Query Frame = 0

Query: 8   LSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDD 67
           LS   +F   I  SA  SS P  V +PELVV+EVHR I  +  RR LG+ SCG+GNPIDD
Sbjct: 29  LSFALIFFCCILFSALASSLP--VSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDD 88

Query: 68  CWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQD 127
           CWRCD +WEKNR+RLADCGIGFGKNAIGGRDG+IYVVTD G+ DPVNP+PGTLRYAVIQD
Sbjct: 89  CWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQD 148

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIF RDM I+LKEELIMNSFKT+DGRGASVHI+GGPCITIQYVTNIIIHGL+IHDC
Sbjct: 149 EPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDC 208

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           K GGNT VRDSP H+G+RT+SDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAI GSTAITI
Sbjct: 209 KQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITI 268

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 269 SNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 328

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+D  SKEVTK+EDAPE EW+NW+WRSEGDL+LNGAF
Sbjct: 329 WEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAF 388

Query: 368 FTSSGAG--ASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FT SGAG   SSSY++ASSL ARPSS VG IT  +GAL+C++GS C
Sbjct: 389 FTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of PI0013949 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 683.3 bits (1762), Expect = 1.7e-195
Identity = 320/382 (83.77%), Postives = 353/382 (92.41%), Query Frame = 0

Query: 31  VPNPELVVQEVHRSI-INATKRRNLGYLSCGTGNPIDDCWRCDSNWEKNRQRLADCGIGF 90
           VP+PE VV+EVH+SI  +   RR LGYLSC TGNPIDDCWRCD +WE++RQRLADC IGF
Sbjct: 27  VPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGF 86

Query: 91  GKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMN 150
           GKNAIGGRDG+IYVVTD+G+ +PV+PKPGTLR+AV+QDEPLWIIF RDM I+LKEELIMN
Sbjct: 87  GKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMN 146

Query: 151 SFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISD 210
           SFKTIDGRGASVHI+GGPCITIQYVTNIIIHG++IHDCK GGN  VR SPRHFG+RTISD
Sbjct: 147 SFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISD 206

Query: 211 GDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQ 270
           GDGVSIFGGSHVWVDHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++
Sbjct: 207 GDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSR 266

Query: 271 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFV 330
           DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRF+
Sbjct: 267 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFL 326

Query: 331 APNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSSGAGASSSYARASSLGARP 390
           APN RFSKEVTK+EDAPESEWK W+WRS GDL+LNGAFFT SG  ASSSYA+ASSLGA+P
Sbjct: 327 APNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKP 386

Query: 391 SSLVGTITTNAGALNCRKGSRC 412
           SSLVG +T+ +GALNCRKGSRC
Sbjct: 387 SSLVGPLTSTSGALNCRKGSRC 408

BLAST of PI0013949 vs. ExPASy Swiss-Prot
Match: Q9FXD8 (Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 682.2 bits (1759), Expect = 3.7e-195
Identity = 334/403 (82.88%), Postives = 358/403 (88.83%), Query Frame = 0

Query: 11  LSLFLFSISLSAALSSAPAAVP--NPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDC 70
           LSL LFS  L     +  A+ P   PELVVQEV+   INA+ RRNLG LSCGTGNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEK-INAS-RRNLGVLSCGTGNPIDDC 67

Query: 71  WRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDE 130
           WRCD  WEKNRQRLADC IGFGK+AIGGRDGKIYVVTD+ D D VNPKPGTLR+AVIQDE
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 131 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 190
           PLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGG CIT+QYVTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 191 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 250
             GN  VRDSP H+G+RT SDGD VSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAITIS
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 251 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 310
           NNY++HH+KVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 311 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 370
           +MYAIGGSAAPTINSQGNRF+APND   KEVTKYEDAP S+WK W+WRSEGDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 371 TSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           T SG GASSSYA+ASSL ARPSSLV ++T+NAGAL CRKGSRC
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of PI0013949 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 648.7 bits (1672), Expect = 4.5e-185
Identity = 307/400 (76.75%), Postives = 347/400 (86.75%), Query Frame = 0

Query: 13  LFLFSISLSAALSSA-PAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDCWRC 72
           L L +I  + A SS+ P  V +P LVV +V+RS+ NA+ RR+L YLSC TGNPIDDCWRC
Sbjct: 14  LLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNAS-RRSLAYLSCRTGNPIDDCWRC 73

Query: 73  DSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLW 132
           D NWE NRQRLADC IGFGKNAIGGR G+IYVVTD  + DPVNP+PGTLRYAV Q+EPLW
Sbjct: 74  DPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPLW 133

Query: 133 IIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGG 192
           IIF RDMVIRLK+ELI+ SFKTIDGRG+SVHI  GPC+ I Y TNIIIHG+NIHDCKPG 
Sbjct: 134 IIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPGS 193

Query: 193 NTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNY 252
              ++D P H G+   SDGD V+IFGG HVW+DHCSLSNC+DGLIDAIHGSTAITISNN+
Sbjct: 194 GGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNNH 253

Query: 253 MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY 312
           MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY
Sbjct: 254 MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY 313

Query: 313 AIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSS 372
           AIGGSA+PTI SQGNRF+APN RF+KEVTK+EDAPES+W++W+WRSEGD++LNGA+F  S
Sbjct: 314 AIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRES 373

Query: 373 GAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GA A S+YARASSL ARPSSLVG+ITT AG L+CR+G RC
Sbjct: 374 GAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of PI0013949 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 8.6e-184
Identity = 310/403 (76.92%), Postives = 351/403 (87.10%), Query Frame = 0

Query: 9   SLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDC 68
           ++L L LF  SL+ A SS+P+  P+   +V EVH+S INA+ RRNLGYLSCGTGNPIDDC
Sbjct: 4   TILPLILFISSLAIA-SSSPSRTPH--AIVNEVHKS-INAS-RRNLGYLSCGTGNPIDDC 63

Query: 69  WRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDE 128
           WRCD NW  NRQRLADC IGFGKNA+GGR+G+IYVVTD G+ DPVNP PGTLRYAVIQDE
Sbjct: 64  WRCDPNWANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDE 123

Query: 129 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 188
           PLWIIF RDMVI+L++EL+MNS KTIDGRG +VHI  GPCITI Y +NIIIHG++IHDCK
Sbjct: 124 PLWIIFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCK 183

Query: 189 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 248
             GN ++R+SP H G+ T SDGDG+SIF    +W+DH SLSNC+DGLIDAIHGSTAITIS
Sbjct: 184 QAGNGNIRNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITIS 243

Query: 249 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 308
           NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 244 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 303

Query: 309 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 368
           EMYAIGGSA+PTI SQGNRF+APN RF KEVTK+E+APESEWKNW+WRSEGDLMLNGA+F
Sbjct: 304 EMYAIGGSASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYF 363

Query: 369 TSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
             SG  A+SS+ARASSL  RPS+LV ++T +AGAL CRKGSRC
Sbjct: 364 RESGGRAASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of PI0013949 vs. ExPASy TrEMBL
Match: A0A5A7UJC7 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004230 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 4.6e-241
Identity = 408/411 (99.27%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTVPLSLLSLFLFSISLSAALSSAPAAV +PELVVQEVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVSDPELVVQEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 411

BLAST of PI0013949 vs. ExPASy TrEMBL
Match: A0A1S3AY03 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483993 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 4.6e-241
Identity = 408/411 (99.27%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTVPLSLLSLFLFSISLSAALSSAPAAV +PELVVQEVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVSDPELVVQEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 411

BLAST of PI0013949 vs. ExPASy TrEMBL
Match: A0A0A0LU82 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G049960 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 5.9e-236
Identity = 401/413 (97.09%), Postives = 405/413 (98.06%), Query Frame = 0

Query: 1   MAPPTVPLSLLS--LFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLS 60
           MAPP +PLSLLS  LFLFSISLSAALS  PAAV NPELVVQEVHRSIINATKRRNLGYLS
Sbjct: 1   MAPPALPLSLLSLFLFLFSISLSAALSPNPAAVSNPELVVQEVHRSIINATKRRNLGYLS 60

Query: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPG 120
           CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTD+GD DPVNPKPG
Sbjct: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPG 120

Query: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180
           TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII
Sbjct: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180

Query: 181 IHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240
           IHGLNIHDCK GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA
Sbjct: 181 IHGLNIHDCKQGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240

Query: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300
           IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF
Sbjct: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300

Query: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360
           HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE
Sbjct: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360

Query: 361 GDLMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GDLMLNGA+FT+SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 GDLMLNGAYFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 413

BLAST of PI0013949 vs. ExPASy TrEMBL
Match: A0A6J1EDH2 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431534 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.7e-233
Identity = 392/411 (95.38%), Postives = 403/411 (98.05%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTV LSLLSLF  S+SLSAALS++PAAVPNPE+VV+EVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVVLSLLSLFFLSVSLSAALSASPAAVPNPEIVVEEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGRIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWI FARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWITFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNW+WRS GD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWNWRSVGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFFT+SGA +SSSYARASSLGARPSSLVGTITTNAGAL+CRKGSRC
Sbjct: 361 LMVNGAFFTASGAESSSSYARASSLGARPSSLVGTITTNAGALSCRKGSRC 411

BLAST of PI0013949 vs. ExPASy TrEMBL
Match: A0A6J1K1J7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111490238 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 4.6e-233
Identity = 395/411 (96.11%), Postives = 403/411 (98.05%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPT+ LSLLSLFL S SLS  LS+APAAV NPELVVQEVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTLSLSLLSLFLLS-SLSTTLSAAPAAVQNPELVVQEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTD+GD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKQGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWK+W+WRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKHWNWRSEGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           +M+NGAFFT+SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 MMVNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 410

BLAST of PI0013949 vs. NCBI nr
Match: XP_008439105.1 (PREDICTED: probable pectate lyase 22 [Cucumis melo] >KAA0055290.1 putative pectate lyase 22 [Cucumis melo var. makuwa] >TYJ99213.1 putative pectate lyase 22 [Cucumis melo var. makuwa])

HSP 1 Score: 843.2 bits (2177), Expect = 9.6e-241
Identity = 408/411 (99.27%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTVPLSLLSLFLFSISLSAALSSAPAAV +PELVVQEVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVSDPELVVQEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 411

BLAST of PI0013949 vs. NCBI nr
Match: XP_038895410.1 (probable pectate lyase 22 [Benincasa hispida])

HSP 1 Score: 835.9 bits (2158), Expect = 1.5e-238
Identity = 400/411 (97.32%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTVPLSLLSLFLFS+SLSAA+S+APAAVPNPELVV EVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVPLSLLSLFLFSVSLSAAISAAPAAVPNPELVVDEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCDSNW KNRQRLADCGIGFGKNAIGGRDG+IYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWVKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDG+SIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGISIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEW+NWHWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWRNWHWRSEGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFFT+SGAGASSSYARASSLGARPSSLVGTITTNAGALNC+KGSRC
Sbjct: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCKKGSRC 411

BLAST of PI0013949 vs. NCBI nr
Match: XP_004152502.1 (probable pectate lyase 5 [Cucumis sativus] >KGN64357.1 hypothetical protein Csa_014078 [Cucumis sativus])

HSP 1 Score: 826.2 bits (2133), Expect = 1.2e-235
Identity = 401/413 (97.09%), Postives = 405/413 (98.06%), Query Frame = 0

Query: 1   MAPPTVPLSLLS--LFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLS 60
           MAPP +PLSLLS  LFLFSISLSAALS  PAAV NPELVVQEVHRSIINATKRRNLGYLS
Sbjct: 1   MAPPALPLSLLSLFLFLFSISLSAALSPNPAAVSNPELVVQEVHRSIINATKRRNLGYLS 60

Query: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPG 120
           CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTD+GD DPVNPKPG
Sbjct: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPG 120

Query: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180
           TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII
Sbjct: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180

Query: 181 IHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240
           IHGLNIHDCK GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA
Sbjct: 181 IHGLNIHDCKQGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240

Query: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300
           IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF
Sbjct: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300

Query: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360
           HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE
Sbjct: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360

Query: 361 GDLMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GDLMLNGA+FT+SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 GDLMLNGAYFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 413

BLAST of PI0013949 vs. NCBI nr
Match: KAG7019707.1 (putative pectate lyase 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 817.4 bits (2110), Expect = 5.6e-233
Identity = 391/411 (95.13%), Postives = 404/411 (98.30%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTV LSLLSLF  S+SLSAA+S++PAAVPNPE+VV+EVHRSIIN+TKRRNLGYLSCG
Sbjct: 1   MAPPTVVLSLLSLFFLSVSLSAAISASPAAVPNPEIVVEEVHRSIINSTKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGRIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNW+WRS GD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWNWRSVGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFFT+SGA +SSSYARASSLGARPSSLVGTITTNAGAL+CRKGSRC
Sbjct: 361 LMVNGAFFTASGAESSSSYARASSLGARPSSLVGTITTNAGALSCRKGSRC 411

BLAST of PI0013949 vs. NCBI nr
Match: XP_022923975.1 (probable pectate lyase 18 [Cucurbita moschata])

HSP 1 Score: 817.4 bits (2110), Expect = 5.6e-233
Identity = 392/411 (95.38%), Postives = 403/411 (98.05%), Query Frame = 0

Query: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCG 60
           MAPPTV LSLLSLF  S+SLSAALS++PAAVPNPE+VV+EVHRSIINATKRRNLGYLSCG
Sbjct: 1   MAPPTVVLSLLSLFFLSVSLSAALSASPAAVPNPEIVVEEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTL 120
           TGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTDAGD DPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGRIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWI FARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWITFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNW+WRS GD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWNWRSVGD 360

Query: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFFT+SGA +SSSYARASSLGARPSSLVGTITTNAGAL+CRKGSRC
Sbjct: 361 LMVNGAFFTASGAESSSSYARASSLGARPSSLVGTITTNAGALSCRKGSRC 411

BLAST of PI0013949 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 686.0 bits (1769), Expect = 1.8e-197
Identity = 328/406 (80.79%), Postives = 361/406 (88.92%), Query Frame = 0

Query: 8   LSLLSLFLFSISLSAALSSAPAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDD 67
           LS   +F   I  SA  SS P  V +PELVV+EVHR I  +  RR LG+ SCG+GNPIDD
Sbjct: 29  LSFALIFFCCILFSALASSLP--VSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDD 88

Query: 68  CWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQD 127
           CWRCD +WEKNR+RLADCGIGFGKNAIGGRDG+IYVVTD G+ DPVNP+PGTLRYAVIQD
Sbjct: 89  CWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQD 148

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIF RDM I+LKEELIMNSFKT+DGRGASVHI+GGPCITIQYVTNIIIHGL+IHDC
Sbjct: 149 EPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDC 208

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           K GGNT VRDSP H+G+RT+SDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAI GSTAITI
Sbjct: 209 KQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITI 268

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 269 SNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 328

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+D  SKEVTK+EDAPE EW+NW+WRSEGDL+LNGAF
Sbjct: 329 WEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAF 388

Query: 368 FTSSGAG--ASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FT SGAG   SSSY++ASSL ARPSS VG IT  +GAL+C++GS C
Sbjct: 389 FTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of PI0013949 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.2e-196
Identity = 320/382 (83.77%), Postives = 353/382 (92.41%), Query Frame = 0

Query: 31  VPNPELVVQEVHRSI-INATKRRNLGYLSCGTGNPIDDCWRCDSNWEKNRQRLADCGIGF 90
           VP+PE VV+EVH+SI  +   RR LGYLSC TGNPIDDCWRCD +WE++RQRLADC IGF
Sbjct: 27  VPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGF 86

Query: 91  GKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMN 150
           GKNAIGGRDG+IYVVTD+G+ +PV+PKPGTLR+AV+QDEPLWIIF RDM I+LKEELIMN
Sbjct: 87  GKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMN 146

Query: 151 SFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISD 210
           SFKTIDGRGASVHI+GGPCITIQYVTNIIIHG++IHDCK GGN  VR SPRHFG+RTISD
Sbjct: 147 SFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISD 206

Query: 211 GDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQ 270
           GDGVSIFGGSHVWVDHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++
Sbjct: 207 GDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSR 266

Query: 271 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFV 330
           DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRF+
Sbjct: 267 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFL 326

Query: 331 APNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSSGAGASSSYARASSLGARP 390
           APN RFSKEVTK+EDAPESEWK W+WRS GDL+LNGAFFT SG  ASSSYA+ASSLGA+P
Sbjct: 327 APNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKP 386

Query: 391 SSLVGTITTNAGALNCRKGSRC 412
           SSLVG +T+ +GALNCRKGSRC
Sbjct: 387 SSLVGPLTSTSGALNCRKGSRC 408

BLAST of PI0013949 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.2e-196
Identity = 320/382 (83.77%), Postives = 353/382 (92.41%), Query Frame = 0

Query: 31  VPNPELVVQEVHRSI-INATKRRNLGYLSCGTGNPIDDCWRCDSNWEKNRQRLADCGIGF 90
           VP+PE VV+EVH+SI  +   RR LGYLSC TGNPIDDCWRCD +WE++RQRLADC IGF
Sbjct: 27  VPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGF 86

Query: 91  GKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMN 150
           GKNAIGGRDG+IYVVTD+G+ +PV+PKPGTLR+AV+QDEPLWIIF RDM I+LKEELIMN
Sbjct: 87  GKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMN 146

Query: 151 SFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISD 210
           SFKTIDGRGASVHI+GGPCITIQYVTNIIIHG++IHDCK GGN  VR SPRHFG+RTISD
Sbjct: 147 SFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISD 206

Query: 211 GDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQ 270
           GDGVSIFGGSHVWVDHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++
Sbjct: 207 GDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSR 266

Query: 271 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFV 330
           DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRF+
Sbjct: 267 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFL 326

Query: 331 APNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSSGAGASSSYARASSLGARP 390
           APN RFSKEVTK+EDAPESEWK W+WRS GDL+LNGAFFT SG  ASSSYA+ASSLGA+P
Sbjct: 327 APNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKP 386

Query: 391 SSLVGTITTNAGALNCRKGSRC 412
           SSLVG +T+ +GALNCRKGSRC
Sbjct: 387 SSLVGPLTSTSGALNCRKGSRC 408

BLAST of PI0013949 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 682.2 bits (1759), Expect = 2.6e-196
Identity = 334/403 (82.88%), Postives = 358/403 (88.83%), Query Frame = 0

Query: 11  LSLFLFSISLSAALSSAPAAVP--NPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDC 70
           LSL LFS  L     +  A+ P   PELVVQEV+   INA+ RRNLG LSCGTGNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEK-INAS-RRNLGVLSCGTGNPIDDC 67

Query: 71  WRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDE 130
           WRCD  WEKNRQRLADC IGFGK+AIGGRDGKIYVVTD+ D D VNPKPGTLR+AVIQDE
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 131 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 190
           PLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGG CIT+QYVTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 191 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 250
             GN  VRDSP H+G+RT SDGD VSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAITIS
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 251 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 310
           NNY++HH+KVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 311 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 370
           +MYAIGGSAAPTINSQGNRF+APND   KEVTKYEDAP S+WK W+WRSEGDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 371 TSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           T SG GASSSYA+ASSL ARPSSLV ++T+NAGAL CRKGSRC
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of PI0013949 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 648.7 bits (1672), Expect = 3.2e-186
Identity = 307/400 (76.75%), Postives = 347/400 (86.75%), Query Frame = 0

Query: 13  LFLFSISLSAALSSA-PAAVPNPELVVQEVHRSIINATKRRNLGYLSCGTGNPIDDCWRC 72
           L L +I  + A SS+ P  V +P LVV +V+RS+ NA+ RR+L YLSC TGNPIDDCWRC
Sbjct: 14  LLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNAS-RRSLAYLSCRTGNPIDDCWRC 73

Query: 73  DSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDYDPVNPKPGTLRYAVIQDEPLW 132
           D NWE NRQRLADC IGFGKNAIGGR G+IYVVTD  + DPVNP+PGTLRYAV Q+EPLW
Sbjct: 74  DPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPLW 133

Query: 133 IIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGG 192
           IIF RDMVIRLK+ELI+ SFKTIDGRG+SVHI  GPC+ I Y TNIIIHG+NIHDCKPG 
Sbjct: 134 IIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPGS 193

Query: 193 NTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNY 252
              ++D P H G+   SDGD V+IFGG HVW+DHCSLSNC+DGLIDAIHGSTAITISNN+
Sbjct: 194 GGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNNH 253

Query: 253 MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY 312
           MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY
Sbjct: 254 MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY 313

Query: 313 AIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTSS 372
           AIGGSA+PTI SQGNRF+APN RF+KEVTK+EDAPES+W++W+WRSEGD++LNGA+F  S
Sbjct: 314 AIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRES 373

Query: 373 GAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GA A S+YARASSL ARPSSLVG+ITT AG L+CR+G RC
Sbjct: 374 GAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z252.6e-19680.79Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Q9C5M81.7e-19583.77Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
Q9FXD83.7e-19582.88Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2[more]
Q9LTZ04.5e-18576.75Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
O245548.6e-18476.92Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UJC74.6e-24199.27Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004230... [more]
A0A1S3AY034.6e-24199.27Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483993 PE=3 SV=1[more]
A0A0A0LU825.9e-23697.09Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G049960 PE=3 SV=1[more]
A0A6J1EDH22.7e-23395.38Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431534 PE=3 SV=1[more]
A0A6J1K1J74.6e-23396.11Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111490238 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008439105.19.6e-24199.27PREDICTED: probable pectate lyase 22 [Cucumis melo] >KAA0055290.1 putative pecta... [more]
XP_038895410.11.5e-23897.32probable pectate lyase 22 [Benincasa hispida][more]
XP_004152502.11.2e-23597.09probable pectate lyase 5 [Cucumis sativus] >KGN64357.1 hypothetical protein Csa_... [more]
KAG7019707.15.6e-23395.13putative pectate lyase 22 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923975.15.6e-23395.38probable pectate lyase 18 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G63180.11.8e-19780.79Pectin lyase-like superfamily protein [more]
AT4G24780.11.2e-19683.77Pectin lyase-like superfamily protein [more]
AT4G24780.21.2e-19683.77Pectin lyase-like superfamily protein [more]
AT1G67750.12.6e-19682.88Pectate lyase family protein [more]
AT3G27400.13.2e-18676.75Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 85..102
score: 56.88
coord: 382..405
score: 40.48
coord: 289..308
score: 78.1
coord: 209..230
score: 56.71
coord: 311..330
score: 78.57
coord: 109..134
score: 69.41
coord: 353..377
score: 51.62
coord: 145..161
score: 71.99
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 137..334
e-value: 8.7E-104
score: 360.8
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 147..328
e-value: 5.2E-21
score: 75.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 62..404
e-value: 5.1E-119
score: 399.3
NoneNo IPR availablePANTHERPTHR31683:SF151PECTATE LYASEcoord: 11..408
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 11..408
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 38..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013949.1PI0013949.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity