PI0013501 (gene) Melon (PI 482460) v1

BLAST of PI0013501 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 729.9 bits (1883), Expect = 1.7e-209
Identity = 356/445 (80.00%), Postives = 389/445 (87.42%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTASGA 450
           SC  K+RV FKG DGV DMDTA GA
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAIGA 454

BLAST of PI0013501 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.7e-105
Identity = 201/409 (49.14%), Postives = 262/409 (64.06%), Query Frame = 0

Query: 3   GSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLA 62
           G ++QS R   L  +L R G      F   S+  + LQ   +VL+IGAGGLGCELLKDLA
Sbjct: 9   GLILQSKRFNGLRNILEREGPFCKDGFAASSENLEFLQTKCQVLIIGAGGLGCELLKDLA 68

Query: 63  LSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIE 122
           L GF NL VIDMD IE++NLNRQFLFR  D+G  KAE AA+ +  RV    + PHF +I+
Sbjct: 69  LMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQ 128

Query: 123 DKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKG 182
           D +  FY  FH++  GLDSI AR +IN +  S L Y+ D      +I PM+DGGTEGFKG
Sbjct: 129 DFDESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKG 188

Query: 183 HARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKAF 242
           +ARVILPG T C ECT+ LFPPQV +PLCT+A TPR   HCIEY  +I+W++ +  G   
Sbjct: 189 NARVILPGFTACIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPL 248

Query: 243 DPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLK 302
           D DD +H+ W+Y  AL+R+  F I GVTY L QGVVK+IIPA+ASTNA I+AACALE  K
Sbjct: 249 DGDDPQHIGWIYERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFK 308

Query: 303 IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCG--PGVL-IELDSSITLQKFIDL 362
           + + C  +++NYL +N  +G++T   E  K ++CL C   P  L IE  ++ TL+  I L
Sbjct: 309 LATSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKL 368

Query: 363 LEDHPKLLLSKASVT-----HRGKNLYMQAPPVLEEMTRSNLGVPLFDL 403
           L D P+  L   ++T      + + LYM     +EE TR NL   L +L
Sbjct: 369 LCDSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIEEATRKNLTQSLGEL 417

BLAST of PI0013501 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.0e-105
Identity = 201/412 (48.79%), Postives = 267/412 (64.81%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQ----LRDDLQQYVKVLVIGAGGLGCE 60
           M  SV    R  D++K++ R G     +FEP ++    + + LQ   KVLVIGAGGLGCE
Sbjct: 4   MDTSVDLPGRWIDIEKIIKRTGPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCE 63

Query: 61  LLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILP 120
           +LK+LALSGFRN++VIDMD I+++NLNRQFLFR +DVGK KAEVAA  +  R++G N+ P
Sbjct: 64  ILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTP 123

Query: 121 HFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGG 180
           H CRI+DK+ ++Y  F I+  GLDSIEAR +IN +  + +  +   +   +TI P+VDGG
Sbjct: 124 HKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGG 183

Query: 181 TEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH 240
           TEGFKG ARVILP ++ CFEC++  FPPQV + +CT+A TPR   HCI++A L    +  
Sbjct: 184 TEGFKGQARVILPKISSCFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDAT 243

Query: 241 SGKAFDP-----DDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAII 300
             K FDP     D+ +HM W++  A KRAE F I GVTY LTQGV KNIIPAIASTNAII
Sbjct: 244 LEKPFDPKQFDNDNPDHMNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAII 303

Query: 301 SAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSI 360
           +AAC  E  K  +  S  L+NY+ YNG  G++T   E+   + C VCG   V  E+D S 
Sbjct: 304 AAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSN 363

Query: 361 TLQKFIDLLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDL 403
           TL  F++ +    +    K S+   G+NLYMQ   +L + T  NL   L +L
Sbjct: 364 TLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

BLAST of PI0013501 vs. ExPASy Swiss-Prot
Match: Q8TBC4 (NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens OX=9606 GN=UBA3 PE=1 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 197/402 (49.00%), Postives = 262/402 (65.17%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDD 276

Query: 250 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+I+A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 403
           L +   ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of PI0013501 vs. ExPASy Swiss-Prot
Match: Q5R4A0 (NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii OX=9601 GN=UBA3 PE=2 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 196/402 (48.76%), Postives = 262/402 (65.17%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDD 276

Query: 250 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+++A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVVAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDFLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 403
           L +   ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of PI0013501 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.1e-259
Identity = 448/449 (99.78%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 448/449 (99.78%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.0e-259
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRV FKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. ExPASy TrEMBL
Match: A0A6J1CA17 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.1e-254
Identity = 436/449 (97.10%), Postives = 444/449 (98.89%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK 
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTA GA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAGGA 449

BLAST of PI0013501 vs. ExPASy TrEMBL
Match: A0A6J1C926 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.4e-253
Identity = 434/446 (97.31%), Postives = 442/446 (99.10%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK 
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of PI0013501 vs. NCBI nr
Match: XP_004134149.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypothetical protein Csa_009619 [Cucumis sativus])

HSP 1 Score: 905.2 bits (2338), Expect = 2.2e-259
Identity = 448/449 (99.78%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. NCBI nr
Match: XP_008438709.1 (PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 448/449 (99.78%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. NCBI nr
Match: KAA0049385.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK17174.1 NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 902.5 bits (2331), Expect = 1.5e-258
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRV FKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 902.1 bits (2330), Expect = 1.9e-258
Identity = 445/449 (99.11%), Postives = 448/449 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGS+VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSIVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKD VHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTASGA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 449

BLAST of PI0013501 vs. NCBI nr
Match: XP_023528363.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.5 bits (2292), Expect = 4.8e-254
Identity = 439/449 (97.77%), Postives = 444/449 (98.89%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MA SVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MADSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETL
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTASGA 450
           DKKTSCLRKLRVAFKGVDGVTDMDTA GA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAGGA 449

BLAST of PI0013501 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 729.9 bits (1883), Expect = 1.2e-210
Identity = 356/445 (80.00%), Postives = 389/445 (87.42%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTASGA 450
           SC  K+RV FKG DGV DMDTA GA
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAIGA 454

BLAST of PI0013501 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 52.0 bits (123), Expect = 1.5e-06
Identity = 42/144 (29.17%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 301 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 360
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 361 LED--HPKLLLSKASVTHRGKNLY 381
           ++     KL ++   + H    LY
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLY 478

BLAST of PI0013501 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 52.0 bits (123), Expect = 1.5e-06
Identity = 42/144 (29.17%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 301 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 360
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 361 LED--HPKLLLSKASVTHRGKNLY 381
           ++     KL ++   + H    LY
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLY 478

BLAST of PI0013501 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 54.7 bits (130), Expect = 2.2e-07
Identity = 46/162 (28.40%), Postives = 75/162 (46.30%), Query Frame = 0

Query: 223 CIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIP 282
           C+E  HL           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ 
Sbjct: 331 CVEIGHL----------TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVH 390

Query: 283 AIASTNAIISAACALETLKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGP 342
           A+A+TNAII+    +E +K++     K    Y L +   + L   +  +  +  C VC  
Sbjct: 391 AVATTNAIIAGLIVIEAIKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSE 450

Query: 343 GVLIELDSSITLQKFIDLLED--HPKLLLSKASVTHRGKNLY 381
             L+ L+ +    K  DL++     KL ++   + H    LY
Sbjct: 451 TPLV-LEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 481

BLAST of PI0013501 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 101.3 bits (251), Expect = 2.1e-21
Identity = 68/206 (33.01%), Postives = 110/206 (53.40%), Query Frame = 0

Query: 32  GSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFR-----NLEVIDMDRIEVTNLNRQF 91
           GS L+  L+   +V V+GAG LGCE LK+LAL G        L V D D IE +NL+RQF
Sbjct: 480 GSTLQKKLED-ARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQF 539

Query: 92  LFRLEDVGKPKAEVAAKRVMERVSGVNI--LPHFCRIEDKEI---EFYNDFHIIALGLDS 151
           LFR  ++G+ K+ VAA       S +NI  L +    E + +    F+ +  ++   LD+
Sbjct: 540 LFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 599

Query: 152 IEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 211
           + AR Y++S  C + +            KP+++ GT G K + ++++P +T  +  +   
Sbjct: 600 VTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIPHLTENYGAS--R 659

Query: 212 FPPQVKFPLCTLAETPRTAAHCIEYA 228
            PP+ + P+CT+   P    HC+ +A
Sbjct: 660 DPPEKQAPMCTVHSFPHNIDHCLTWA 669