PI0013404 (gene) Melon (PI 482460) v1

Overview
NamePI0013404
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMembrane protein of ER body-like protein
Locationchr01: 21734749 .. 21739599 (-)
RNA-Seq ExpressionPI0013404
SyntenyPI0013404
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATTATTAAAATAATAGCAAGTGGCCATTTCTTCTTCACTTCACATTTCTAAAATATGCCAATAAAAACCACTCCGTTCCGCCCTTTTTTCTTCTCTCTCCATACACCCTTAAGCTTCCCACACCCATATTCTCTCCTCTAACCCACCCTACCTTGCTTGTAATGGAAGACGTTGTAGATTTGCTGCCGCCGCCTGTGGAACCGGTTGCCGGAGATACCAAGTCACGTGAGAAGCACCTGATCAGATCAGGCTCCTCTTCTGATAGCGATGAAATGTATTCCGCCGGATCTCCTAAAGGTATATATTTTTCATTTTCCTCACCTACTTCCTCTTTAACATCCTATGTTTTGTTGAGTTATAATCGTTTTCGCACCTCACCTCAATATGTATTTAATCTAATTTTGTTAACATTTTTATAAACGTTTCAAAACCATGATTTTAGAGTTAATATTCGTTTTAAAATTTTGTTCAACTTAGAATTCACGAGTTAAAATGATGTGATCATTTAAATTTTCTATTAAATATTACCTAACTACAACGCCATTTTATTTTTAAATTTTCGTCCAAAATTTTTAACTATTTTATATTTAAGTGACATTTTTGGAATACTTATAAAAGTTGATTTGGTTGAGCAAACCTCTTTTTTTTTTTTTTTTTCTATTTATAGGAATCATACTTGTTATTATATTTTCATTAAGTTGTTACTAAAATTAGTCCATTTATTTACAAATTTATTAGTGTTAGATAACAATAATAAACGACATTAATAAAGTCGTGAGTGACATAGATAGATACTCTATCCGGAACTATTGTTATCTATTACTAATAGATATGTATTTTACTATATTTAAAAAGTATTTTCATTTTCAGTAATTTTGTCATTTAAAACAATGTAACGAAAGTCAACTTTCCTACCTATATTTTTCCTATTTTGGTAATCAATAAGTTACATTTTTGGGTTTATGGACTTCCTTTTTGTCTTTCTTATTTTGTTTCTAAACTATATATGTACATAAAGAAAATACAATACAACAATTTTCAGGTTGTTGATGTAGGATCTGTATTAGAGTGTGTGTAATACTATTTTTATAAGATAATACGATATCAAAATTTGTTAATCTCAAAAATTTAAATACTAATTAAAATAAATATTTTGGATGTTTATATTCTATGGTTGATTGATGTAAAATAGATTTTTTAACTTTGGATGTGTCAAATAGTTCCTAAAATATTTTATAATTTATGTTTAATACATATTTCTAAATTTTAAAAACTATTTATTGAGTTCCTCCAACTTTCAAATTTTATGTCTAAAAATTTTATGAATATATTATCGAATTAAAATTGATAAAAAAATTTTAATTGAGGACACCATCAAACACACAAAATTCAATTTTAAGTTTATTAATTAATTTTTAAAATGTCAACCTATTAAAAAAGAACAGACTAAAATGTAAAGACTCATTAGACATGTACAATGTCAAAGTTTAAGAAATAAACTTGTAATTTAATGAGCAAAATTTTATGAGCAAAATTTTATTTTAGGCATTTGTGTGAATGTTTAATGATAAAAGATATATATTATTTTGGTCATTTAAAATTGTTCAGTAATAAACTACACTCCCATAGATATTAATAAACTCTTATTAGTTTCCATAAGTTTTTCATTAATAGAAAATAAAGGTATTGAAAGCTAAAGAAAATAAATTGAGGACAAAATATTATTGTTTAAGCATAAAATCTCAAAGTGATGGTTACATATATAAAATGATCTTATTTTGGCCATTCAATTCTATCCACTTAAAATTTCCATCTAATATATAGAGGAAAATTCTCTTTCAAAATAAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCCACCATCCTAAAGAGACAACCTTTCACTTTCTCTCTCTTTTTATTTATTTATTAATCAATTTTAATTGTTTGTGAAGTAAGAGAAATATAGTACCTCTTTGAGATATGAATTAGTGTGTTAGAGCATATTCAATATCTATACTTTTAAAATATATATATTATATAATTTTAAGTTTTATATAAATATACAACAAATTTGACTTCTATTATTGTATTTCATATTGTATTTTTGAATTTCAACGTGATTTTGATAATACTATTTAGTAAAATTTTAAATATATTTAAGTGTCTAATTTATGCATTTAGACAAACTTTTTTTTTTTTGTTTTAATTTTTAAAATTAATTATTCAGTTTGTGTGTGATTGAATTAGTAGAATGCCCAAAAAACAATGTTTGAGAAGATGATTATATCAATAATACTAATAATTTATTATTTTTTATGGATTAGAAATTCTGAAATCGGGTGGCAAAGCAATTGCTCCAACTGGAGAAGCCGATATCATTTACATTCCACATGTGCTTGACAAGACTCTAATTTTAAACCGGAATAATAGCAATAAAAATATCAACAATAATTTGACGCCGCCTTCAACCGTCTCGCTTCTCGACATCGGTGGCGGCAACACCGTCACTGAAGTTGTATATCGAAAACCATTTCAAGACGGACAGGAACTAGACTTGCAAACTCTCTACAACTTACCTAATTCCCACAACTTCTTTTGCCCCAACTGCAAATCTTGCATTACAAAAGTCATCATTCTCCGCGACGCCCCATCCTCGCCGCCGCGCTCTCCTCGACCGCAGTTGGGTTCGAAGGATATAGAATATGAGCTACCGCCATCAACTAATATACCGACTGTGACGACGCGGCCAAGGAGCCCAAGCCCCCGACCAGGCGTCATCGAAGAGGCTGGTGTGGATGATGGTGAGATCATTTCCAGGGAAGAGGGTTGTGGAAGGATCATATGCAGCAATTGCTTTAGTTTTCTTAGTCCAATTGGTGAGCTCTTCATATTTTTCTTCCTATCGTTCTATAGTTGATTGATTCTTTAACTTTTTATTTCTTTTTAATTTTCTAATATGAGATTTTTTTTATTATTAGACTAATTTAATTAACCTTCGTCTTCTACTTATTATTATTAGTATGTAGACTTTTCTTCATGTCTTTACAACCCAAGCCAGGTTGTCAAAAAATAGAAAACAAATAGTTTTTGTAAGTTTAGTCTAGATTTAGTATATTATATATATATATATTCATTTAAAAAACAAAAAGTGAAAAAGACAGTGTTCACAAATACCGTTGAATTATTAATTGTTTCAAAATAAATATCTAAAGATCAAACCCTTCGCAAATCTACTTCATTAACTGAAAACAAAAAAAATTGTTAATATTATATATATATATATCAAATAATTTATTAATTGGTTATGTTTCTAACAATTTTTTTTGCCTGATTATTAAGGTGCTTGGATCTCCTCTCAGCTAGGATTTGGTCAGAAAAAGCCAGCAATTTCAGTAAAAATAGGTACTATGATATAATAAAATAAACCAAAATATTTATAAACATTACCAAATATTATTCAATATTTTCAGTAATTTTATATGGTATAATTCATTTCTTAATATTTATCGTTTCAAAATTTAATGTATATTTAAAAATAGACCCAAAAATTGTTAAGAAAAAAAAAAAAGAAGAAGAAGCAAAATTAATCGAAACTTTGAAATTGCAACAACAGTAGGAGGAACTGAAGCTACAAGCCATGCTGGTCCTAGTATTATTTCTCGTGATCAACCATTCATTGAAGTCGAAGGTGGCGACTCGATCGTCCTTACAATTCCTCCGGTCAGCGGGGAAGAAACATTGGGCCAAAGAGGAGGAAGAAGTGTAGAGATAATGAAGAGCATTGTGTATGGAGGTTTAACTGAAGCCATCACAAGTTTGGGCATTGTAACATCTGCAGCAAGTGCGAGTACTCCAACAGGTAACATTTGAAAATTCTTTTAAATCTTGTTTGATAACTATTTTGAAATATTTTCTATATTTTATAAATAATTTCAGCAATTTTACCATTTACAATAATTTTTCTATATTTTGTGATGACCTAATTATATAGATTTGGTATGTAAATGGATTATTTGCAGAGAATATTGTAGCTTTGGCATTGGCAAACTTGATTACGGGTCTTATTGTCATCACAAACAATGTAAGTACAAAAATTACATGGGTTAGAACAATAAATAGTTTAGAGATCGAAAACCCTAATTAAATGGGTTAATTATTTCACAGATCTCTGGACTCAAATCAAACCAACTCAAGAAAGAATCAAACCAAAGTGATGATCAAGCACAAGTAGATCCATATGAAGAAGCTTTAGGAGACAGACACCATTACCTTCTTCACTTCACAACAGCCATCCTATCATTCTTAATCTTCGGGCTTCTGCCACCTCTCGTTTATGGTTTCTCGTTCCGCGACACAGATGACAGCGATCTCAAACTTGCAGCCGTGGCAGTCTCCTCTCTTCTGTGCATTGCCTTACTCGCAATCGTGAAAGCTTATGTTCAAAAGGCAAACAATTGGCAAGAGTATGCCAAAACCCTAGTTTATTACATTACTTTAGGGTTTGGGGCCTCAGGTTTGTCATACTTAGCAGGGAAGGAGTTTAATATCCTTCTTGAGCAACTTGGTTGGTTTAAGAAGGACCAAAGTGCCCCAACTTTGTTGCTCCCTAATATGGACTTGGCAAAACCTACATGGGTATCCATATGAATTGAATGGATGGGCTATGTAGTTGCAAAAGTTAATAGGGGGTTTGTTTAATTTTGTTTTTGTTGAGTGATTAAGATATTTTCTATTGGGTGTGTTCTGTGGTTTCATAAGGGTAATTATTAATTAGAAGAGAACATAAGGGTTTATGTATGTATTGTGTAGTATTTATTTTAATTTGGGCCTTTTTTGTTCTTGAGAA

mRNA sequence

TTTATTATTAAAATAATAGCAAGTGGCCATTTCTTCTTCACTTCACATTTCTAAAATATGCCAATAAAAACCACTCCGTTCCGCCCTTTTTTCTTCTCTCTCCATACACCCTTAAGCTTCCCACACCCATATTCTCTCCTCTAACCCACCCTACCTTGCTTGTAATGGAAGACGTTGTAGATTTGCTGCCGCCGCCTGTGGAACCGGTTGCCGGAGATACCAAGTCACGTGAGAAGCACCTGATCAGATCAGGCTCCTCTTCTGATAGCGATGAAATGTATTCCGCCGGATCTCCTAAAGAAATTCTGAAATCGGGTGGCAAAGCAATTGCTCCAACTGGAGAAGCCGATATCATTTACATTCCACATGTGCTTGACAAGACTCTAATTTTAAACCGGAATAATAGCAATAAAAATATCAACAATAATTTGACGCCGCCTTCAACCGTCTCGCTTCTCGACATCGGTGGCGGCAACACCGTCACTGAAGTTGTATATCGAAAACCATTTCAAGACGGACAGGAACTAGACTTGCAAACTCTCTACAACTTACCTAATTCCCACAACTTCTTTTGCCCCAACTGCAAATCTTGCATTACAAAAGTCATCATTCTCCGCGACGCCCCATCCTCGCCGCCGCGCTCTCCTCGACCGCAGTTGGGTTCGAAGGATATAGAATATGAGCTACCGCCATCAACTAATATACCGACTGTGACGACGCGGCCAAGGAGCCCAAGCCCCCGACCAGGCGTCATCGAAGAGGCTGGTGTGGATGATGGTGAGATCATTTCCAGGGAAGAGGGTTGTGGAAGGATCATATGCAGCAATTGCTTTAGTTTTCTTAGTCCAATTGGTGCTTGGATCTCCTCTCAGCTAGGATTTGGTCAGAAAAAGCCAGCAATTTCAGTAAAAATAGTAGGAGGAACTGAAGCTACAAGCCATGCTGGTCCTAGTATTATTTCTCGTGATCAACCATTCATTGAAGTCGAAGGTGGCGACTCGATCGTCCTTACAATTCCTCCGGTCAGCGGGGAAGAAACATTGGGCCAAAGAGGAGGAAGAAGTGTAGAGATAATGAAGAGCATTGTGTATGGAGGTTTAACTGAAGCCATCACAAGTTTGGGCATTGTAACATCTGCAGCAAGTGCGAGTACTCCAACAGAGAATATTGTAGCTTTGGCATTGGCAAACTTGATTACGGGTCTTATTGTCATCACAAACAATATCTCTGGACTCAAATCAAACCAACTCAAGAAAGAATCAAACCAAAGTGATGATCAAGCACAAGTAGATCCATATGAAGAAGCTTTAGGAGACAGACACCATTACCTTCTTCACTTCACAACAGCCATCCTATCATTCTTAATCTTCGGGCTTCTGCCACCTCTCGTTTATGGTTTCTCGTTCCGCGACACAGATGACAGCGATCTCAAACTTGCAGCCGTGGCAGTCTCCTCTCTTCTGTGCATTGCCTTACTCGCAATCGTGAAAGCTTATGTTCAAAAGGCAAACAATTGGCAAGAGTATGCCAAAACCCTAGTTTATTACATTACTTTAGGGTTTGGGGCCTCAGGTTTGTCATACTTAGCAGGGAAGGAGTTTAATATCCTTCTTGAGCAACTTGGTTGGTTTAAGAAGGACCAAAGTGCCCCAACTTTGTTGCTCCCTAATATGGACTTGGCAAAACCTACATGGGTATCCATATGAATTGAATGGATGGGCTATGTAGTTGCAAAAGTTAATAGGGGGTTTGTTTAATTTTGTTTTTGTTGAGTGATTAAGATATTTTCTATTGGGTGTGTTCTGTGGTTTCATAAGGGTAATTATTAATTAGAAGAGAACATAAGGGTTTATGTATGTATTGTGTAGTATTTATTTTAATTTGGGCCTTTTTTGTTCTTGAGAA

Coding sequence (CDS)

ATGGAAGACGTTGTAGATTTGCTGCCGCCGCCTGTGGAACCGGTTGCCGGAGATACCAAGTCACGTGAGAAGCACCTGATCAGATCAGGCTCCTCTTCTGATAGCGATGAAATGTATTCCGCCGGATCTCCTAAAGAAATTCTGAAATCGGGTGGCAAAGCAATTGCTCCAACTGGAGAAGCCGATATCATTTACATTCCACATGTGCTTGACAAGACTCTAATTTTAAACCGGAATAATAGCAATAAAAATATCAACAATAATTTGACGCCGCCTTCAACCGTCTCGCTTCTCGACATCGGTGGCGGCAACACCGTCACTGAAGTTGTATATCGAAAACCATTTCAAGACGGACAGGAACTAGACTTGCAAACTCTCTACAACTTACCTAATTCCCACAACTTCTTTTGCCCCAACTGCAAATCTTGCATTACAAAAGTCATCATTCTCCGCGACGCCCCATCCTCGCCGCCGCGCTCTCCTCGACCGCAGTTGGGTTCGAAGGATATAGAATATGAGCTACCGCCATCAACTAATATACCGACTGTGACGACGCGGCCAAGGAGCCCAAGCCCCCGACCAGGCGTCATCGAAGAGGCTGGTGTGGATGATGGTGAGATCATTTCCAGGGAAGAGGGTTGTGGAAGGATCATATGCAGCAATTGCTTTAGTTTTCTTAGTCCAATTGGTGCTTGGATCTCCTCTCAGCTAGGATTTGGTCAGAAAAAGCCAGCAATTTCAGTAAAAATAGTAGGAGGAACTGAAGCTACAAGCCATGCTGGTCCTAGTATTATTTCTCGTGATCAACCATTCATTGAAGTCGAAGGTGGCGACTCGATCGTCCTTACAATTCCTCCGGTCAGCGGGGAAGAAACATTGGGCCAAAGAGGAGGAAGAAGTGTAGAGATAATGAAGAGCATTGTGTATGGAGGTTTAACTGAAGCCATCACAAGTTTGGGCATTGTAACATCTGCAGCAAGTGCGAGTACTCCAACAGAGAATATTGTAGCTTTGGCATTGGCAAACTTGATTACGGGTCTTATTGTCATCACAAACAATATCTCTGGACTCAAATCAAACCAACTCAAGAAAGAATCAAACCAAAGTGATGATCAAGCACAAGTAGATCCATATGAAGAAGCTTTAGGAGACAGACACCATTACCTTCTTCACTTCACAACAGCCATCCTATCATTCTTAATCTTCGGGCTTCTGCCACCTCTCGTTTATGGTTTCTCGTTCCGCGACACAGATGACAGCGATCTCAAACTTGCAGCCGTGGCAGTCTCCTCTCTTCTGTGCATTGCCTTACTCGCAATCGTGAAAGCTTATGTTCAAAAGGCAAACAATTGGCAAGAGTATGCCAAAACCCTAGTTTATTACATTACTTTAGGGTTTGGGGCCTCAGGTTTGTCATACTTAGCAGGGAAGGAGTTTAATATCCTTCTTGAGCAACTTGGTTGGTTTAAGAAGGACCAAAGTGCCCCAACTTTGTTGCTCCCTAATATGGACTTGGCAAAACCTACATGGGTATCCATATGA

Protein sequence

MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGEADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPSTNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRIICSNCFSFLSPIGAWISSQLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI
Homology
BLAST of PI0013404 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.3e-35
Identity = 123/408 (30.15%), Postives = 196/408 (48.04%), Query Frame = 0

Query: 103 GNTVTEVVYRKPFQDGQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPR 162
           GN   E   R      ++ +L+ L     ++  +CP+C +CIT+ ++L           +
Sbjct: 179 GNEDEEKNERSSSDSEEKSNLENLLATQENYELYCPSCSTCITRNVVL-----------K 238

Query: 163 PQLGSKDIEYELPPSTNIPTVTTRPRSP-------SPRPGVIEEAGVDDGEIISREEGCG 222
            +   K +   L    +IP V   P  P       SP    + E  ++D     +E+  G
Sbjct: 239 KRKRGKHVNSSLDLKPDIPVV--EPDEPSDIEEMESPVKVYVPETRIED----DQEDKEG 298

Query: 223 RII-CSNC-FSFLSPIGAWISSQLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIE 282
            I  C  C   +   +G     QL + + KP                   +    + +I+
Sbjct: 299 TIFTCLVCDLKYFIRLGTKF-LQLDYIRGKP-------------------VEKSVEEYID 358

Query: 283 VEGGDSIVLTIPPVSGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTE 342
           V    +   + P +  +   G+R   ++E++KS VYGGLTE ITSLG+V+SA+++ + T 
Sbjct: 359 VRKSINTTQSPPQIQPD---GER--FAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 418

Query: 343 NIVALALANLITGLIVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFT 402
           NI+ALA+ANL  GLIV+  N   L+        N SD +   D YEE LG R    +H  
Sbjct: 419 NILALAVANLAGGLIVLAQNFQDLR--------NSSDQEK--DRYEELLGRRTKSRIHIL 478

Query: 403 TAILSFLIFGLLPPLVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANN--- 462
            A++S++ FGL+PPLVY FSF +T   + KL +V + SL+C+ LL  +K YV+K  N   
Sbjct: 479 VAVMSYIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCG 534

Query: 463 -WQEYAKTLVYYITLGFGASGLSYLAGKEFNILLEQLGWFKKDQSAPT 498
             + Y K+  YY ++   + G+SY+ G      +E+L     DQ + T
Sbjct: 539 STKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKLSLVGLDQISIT 534

BLAST of PI0013404 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.7e-35
Identity = 89/195 (45.64%), Postives = 128/195 (65.64%), Query Frame = 0

Query: 298 GRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGL 357
           GR +EI+KSIVYGGL EAITSLG+++SAA +     NI+ L LANL+ GLI+I +N+  L
Sbjct: 540 GRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQEL 599

Query: 358 KSNQ---LKKESNQSDDQAQVD-PYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFS 417
           +  +      E NQ++ + + +  Y+  LG R ++ LH T AILSF+I G+LPP+VY FS
Sbjct: 600 REEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFS 659

Query: 418 FRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSY 477
           F +  + D K+A+V  +SL CI LLAI KA+V+       Y K+++YY ++    SG+SY
Sbjct: 660 FSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRG--SYLKSILYYGSIAVSVSGISY 719

Query: 478 LAGKEFNILLEQLGW 489
           + G     LLE+ GW
Sbjct: 720 VVGNFLEQLLEKHGW 732

BLAST of PI0013404 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 8.3e-35
Identity = 93/201 (46.27%), Postives = 129/201 (64.18%), Query Frame = 0

Query: 295 QRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNI 354
           ++GG  VEI+KSIVYGGLTE+ITSL  VTSAA++   T N++AL +ANL +GL++  +++
Sbjct: 405 RKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSL 464

Query: 355 SGLKSNQLKKESNQSDD----QAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVY 414
             L + + +K++N  D     + + D YEE LG R +  +H   AI SF+IFGL+PPLVY
Sbjct: 465 QELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVY 524

Query: 415 GFSFRDTDD--SDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGA 474
           GFSFR   +   + K+ AV   SLLCI LL+I KAYV K    ++Y KTL  Y T    A
Sbjct: 525 GFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK---RDYVKTLFRYTTTATTA 584

Query: 475 SGLSYLAGKEFNILLEQLGWF 490
           SG S   G   +  LE+ G++
Sbjct: 585 SGFSQFVGYLVSQWLEKSGFY 602

BLAST of PI0013404 vs. ExPASy TrEMBL
Match: A0A5D3CJ51 (Membrane protein of ER body-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00390 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.6e-260
Identity = 471/517 (91.10%), Postives = 485/517 (93.81%), Query Frame = 0

Query: 1   MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGE 60
           MEDVVDLLPPPVEPVAGD KSREKHL RS SSSDSDEMYS GSPKEILKSGGKAIAPTGE
Sbjct: 1   MEDVVDLLPPPVEPVAGDGKSREKHLTRSCSSSDSDEMYSGGSPKEILKSGGKAIAPTGE 60

Query: 61  ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQE 120
           ADIIYIPHV+DKTLILNRNNSNKNINNNLTPPSTVS+LDIGGGN VTE+VYRKPF+DGQE
Sbjct: 61  ADIIYIPHVVDKTLILNRNNSNKNINNNLTPPSTVSVLDIGGGNNVTEIVYRKPFEDGQE 120

Query: 121 LDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEY-ELPPSTN 180
           LDLQTLY LPNSHNFFCPNCKSCITKVIILRD PSSP RSPRPQ GSKD EY +LP STN
Sbjct: 121 LDLQTLYKLPNSHNFFCPNCKSCITKVIILRDPPSSPSRSPRPQSGSKDREYDQLPSSTN 180

Query: 181 IPT-VTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRIICSNCFSFLSPIGAWISSQLG 240
           +PT +TTRP SPSPRP V++E GVDDGEIIS EEG GRIICSNCFSFL+PIGAWISSQLG
Sbjct: 181 VPTEITTRPWSPSPRPSVVQETGVDDGEIISSEEGGGRIICSNCFSFLTPIGAWISSQLG 240

Query: 241 FGQKKPAISVKIVGGTEATSHAGPSIISR-DQPFIEVEGGDSIVLTIPPVSGEETLGQRG 300
           FGQKKP I   IVGGTEATSHAGPSIISR DQPFIEVEGGDSIVLTIPPVSGEETLGQRG
Sbjct: 241 FGQKKPTIPADIVGGTEATSHAGPSIISRDDQPFIEVEGGDSIVLTIPPVSGEETLGQRG 300

Query: 301 GRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGL 360
           GR VEIMKSIVYGGLTEAITSLGIV SAASASTPTENIVALALANLITGLIVITNN+SGL
Sbjct: 301 GRRVEIMKSIVYGGLTEAITSLGIVASAASASTPTENIVALALANLITGLIVITNNLSGL 360

Query: 361 KSNQLKKESNQSDDQA-QVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD 420
           KSNQLKKESNQ+DD A QVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD
Sbjct: 361 KSNQLKKESNQNDDGAVQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD 420

Query: 421 TDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAG 480
           TDD DLKLAAVAVSSLLCIALLAIVKAYVQKANN QEYAKTLVYY++LGFGASGLSYLAG
Sbjct: 421 TDDGDLKLAAVAVSSLLCIALLAIVKAYVQKANNLQEYAKTLVYYVSLGFGASGLSYLAG 480

Query: 481 KEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           KE NILLEQLGWFKKDQS PTLLLPNMDLAKPTW SI
Sbjct: 481 KEVNILLEQLGWFKKDQSTPTLLLPNMDLAKPTWGSI 517

BLAST of PI0013404 vs. ExPASy TrEMBL
Match: A0A0A0K8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G352500 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.3e-253
Identity = 460/526 (87.45%), Postives = 481/526 (91.44%), Query Frame = 0

Query: 1   MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGE 60
           MEDVVD LPPPVEP+AGD K REKH+ RSGSSSDSD+MYS GSPKEILKSGGKAIAPTGE
Sbjct: 1   MEDVVDFLPPPVEPIAGDGKPREKHMTRSGSSSDSDDMYSGGSPKEILKSGGKAIAPTGE 60

Query: 61  ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQE 120
           ADIIYIPHV+DKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPF+DGQE
Sbjct: 61  ADIIYIPHVVDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFEDGQE 120

Query: 121 LDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEY-ELPPSTN 180
           LDLQTLY LPNSHNFFCPNCKSCITKVIILRD PSSP RSP+PQ GSKD EY +LP STN
Sbjct: 121 LDLQTLYKLPNSHNFFCPNCKSCITKVIILRDPPSSPSRSPQPQWGSKDREYDQLPSSTN 180

Query: 181 I-PTVTTRPRSPS--------PRPGVIEEAG-VDDGEIISREEGCGRIICSNCFSFLSPI 240
           I P +TTR RSPS        PRPGV+++ G +DD EIISREEG GRIICSNCFSFL+PI
Sbjct: 181 IPPEITTRARSPSPSPSPSPGPRPGVVQQTGALDDNEIISREEGGGRIICSNCFSFLTPI 240

Query: 241 GAWISSQLGFGQKKPAISVKIVGG-TEATSHAGPSIISRD-QPFIEVEGGDSIVLTIPPV 300
           GAWISSQLGFG KKP I   IVGG TE TS AGPSIISRD QPFI++EGG+SI  TIPP 
Sbjct: 241 GAWISSQLGFGPKKPTIPADIVGGITEGTSRAGPSIISRDGQPFIDIEGGESIAFTIPPA 300

Query: 301 SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL 360
           SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL
Sbjct: 301 SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL 360

Query: 361 IVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPP 420
           IVITNN+SGLKSNQLKKESNQSDD+AQVDPYEEALGDRHHYLLHFTTA+LSFLIFGLLPP
Sbjct: 361 IVITNNLSGLKSNQLKKESNQSDDRAQVDPYEEALGDRHHYLLHFTTAVLSFLIFGLLPP 420

Query: 421 LVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFG 480
           LVYGFSFRDTDD DLKLAAVA SSLLCIALLAI KAYVQKANNW+EYAKTLVYY+TLGFG
Sbjct: 421 LVYGFSFRDTDDGDLKLAAVAASSLLCIALLAIAKAYVQKANNWKEYAKTLVYYVTLGFG 480

Query: 481 ASGLSYLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           ASGLSYLAGKE NILLEQLGWFKKDQS PTLLLPNMDLAKPTW SI
Sbjct: 481 ASGLSYLAGKEVNILLEQLGWFKKDQSVPTLLLPNMDLAKPTWGSI 526

BLAST of PI0013404 vs. ExPASy TrEMBL
Match: A0A6J1JNK9 (membrane protein of ER body 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488504 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 1.3e-160
Identity = 341/521 (65.45%), Postives = 376/521 (72.17%), Query Frame = 0

Query: 1   MEDVVDLLP--PPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPT 60
           ME V+D L     VE  A D KSR+K L R+ SSSDSDEMYS GSPKEILK GGKAIAPT
Sbjct: 1   MEAVIDQLAAVEAVEAAADDRKSRKKQLSRT-SSSDSDEMYSGGSPKEILKLGGKAIAPT 60

Query: 61  GE-ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQD 120
           GE ADII+IPHV+DKTLILNR N     NN+  PP  VS +  GGG  V +  Y KP + 
Sbjct: 61  GEAADIIFIPHVIDKTLILNRGN-----NNSTPPPYDVSDIGGGGGGAVGD-GYPKPIEG 120

Query: 121 GQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPS 180
            QELDLQTLY LP+SHNF+CP CK CITKVII RD PS                    P 
Sbjct: 121 EQELDLQTLYKLPDSHNFYCPTCKCCITKVIIRRDPPS--------------------PR 180

Query: 181 TNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRI--ICSNCFSFLSPIGAWISS 240
           +++     RPRSP P  G      V D       +  GRI  +CSNCFSFL+PIG W+SS
Sbjct: 181 SDVTPTEIRPRSPGPDAG---RTDVRDDVFNPEGDHGGRIDVLCSNCFSFLAPIGKWMSS 240

Query: 241 QLGFGQKKPAI-SVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLG 300
              F ++KP   + K   GT+ TS AGPS  +  +P I+ EG  S V+ IPP  G E LG
Sbjct: 241 L--FRREKPQFPAEKAPPGTDTTSGAGPS-TTPGEPDIKDEGPPSFVIPIPPGDGGERLG 300

Query: 301 Q--RGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360
           Q   GGRS+EIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN
Sbjct: 301 QTVSGGRSLEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360

Query: 361 NISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGF 420
           NISGLKSN+LKKE N+   +A+VD YEEALGDR HYLLHFT A+LSFLIFGLLPPLVYGF
Sbjct: 361 NISGLKSNKLKKEPNER-GEAKVDSYEEALGDRQHYLLHFTIAMLSFLIFGLLPPLVYGF 420

Query: 421 SFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLS 480
           SFR+TDD DLKLAAVA SSLLCIALLAI KAY Q+A NWQ YAKTLVYYITLGFGASGLS
Sbjct: 421 SFRNTDDGDLKLAAVAASSLLCIALLAIAKAYAQRARNWQAYAKTLVYYITLGFGASGLS 480

Query: 481 YLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           YLAGKEF+ LLEQ GWFK D   PTL LPNMD A PTW SI
Sbjct: 481 YLAGKEFDKLLEQFGWFKGDPLPPTLSLPNMDFATPTWGSI 487

BLAST of PI0013404 vs. ExPASy TrEMBL
Match: A0A6J1JXX6 (membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488504 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.9e-160
Identity = 340/520 (65.38%), Postives = 375/520 (72.12%), Query Frame = 0

Query: 1   MEDVVDLLP--PPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPT 60
           ME V+D L     VE  A D KSR+K L R+ SSSDSDEMYS GSPKEILK GGKAIAPT
Sbjct: 1   MEAVIDQLAAVEAVEAAADDRKSRKKQLSRT-SSSDSDEMYSGGSPKEILKLGGKAIAPT 60

Query: 61  GE-ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQD 120
           GE ADII+IPHV+DKTLILNR N     NN+  PP  VS +  GGG  V +  Y KP + 
Sbjct: 61  GEAADIIFIPHVIDKTLILNRGN-----NNSTPPPYDVSDIGGGGGGAVGD-GYPKPIEG 120

Query: 121 GQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPS 180
            QELDLQTLY LP+SHNF+CP CK CITKVII RD PS                    P 
Sbjct: 121 EQELDLQTLYKLPDSHNFYCPTCKCCITKVIIRRDPPS--------------------PR 180

Query: 181 TNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRI--ICSNCFSFLSPIGAWISS 240
           +++     RPRSP P  G      V D       +  GRI  +CSNCFSFL+PIG W+SS
Sbjct: 181 SDVTPTEIRPRSPGPDAG---RTDVRDDVFNPEGDHGGRIDVLCSNCFSFLAPIGKWMSS 240

Query: 241 QLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLGQ 300
              F ++KP    +   GT+ TS AGPS  +  +P I+ EG  S V+ IPP  G E LGQ
Sbjct: 241 L--FRREKPQFPAE--KGTDTTSGAGPS-TTPGEPDIKDEGPPSFVIPIPPGDGGERLGQ 300

Query: 301 --RGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN 360
              GGRS+EIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN
Sbjct: 301 TVSGGRSLEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN 360

Query: 361 ISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFS 420
           ISGLKSN+LKKE N+   +A+VD YEEALGDR HYLLHFT A+LSFLIFGLLPPLVYGFS
Sbjct: 361 ISGLKSNKLKKEPNER-GEAKVDSYEEALGDRQHYLLHFTIAMLSFLIFGLLPPLVYGFS 420

Query: 421 FRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSY 480
           FR+TDD DLKLAAVA SSLLCIALLAI KAY Q+A NWQ YAKTLVYYITLGFGASGLSY
Sbjct: 421 FRNTDDGDLKLAAVAASSLLCIALLAIAKAYAQRARNWQAYAKTLVYYITLGFGASGLSY 480

Query: 481 LAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           LAGKEF+ LLEQ GWFK D   PTL LPNMD A PTW SI
Sbjct: 481 LAGKEFDKLLEQFGWFKGDPLPPTLSLPNMDFATPTWGSI 484

BLAST of PI0013404 vs. ExPASy TrEMBL
Match: A0A6J1GPB5 (membrane protein of ER body 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455908 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 6.5e-160
Identity = 341/521 (65.45%), Postives = 376/521 (72.17%), Query Frame = 0

Query: 1   MEDVVDLLP--PPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPT 60
           ME V+D L     VE  A D KSR+K L R+ SSSDSDEMYS GSPKEILK GGKAIAPT
Sbjct: 1   MEAVIDQLAAVEAVEAAADDRKSRKKQLSRT-SSSDSDEMYSGGSPKEILKLGGKAIAPT 60

Query: 61  GE-ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQD 120
           GE ADII+IPHV+DKTLILNR N     NN+  PP  VS +  GGG  V +  Y KP + 
Sbjct: 61  GEAADIIFIPHVIDKTLILNRGN-----NNSTPPPYDVSDIGGGGGGAVGD-GYPKPIEG 120

Query: 121 GQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPS 180
            QELDLQTLYN+P+SHNF+CP C  CITKVII RD P                   LP S
Sbjct: 121 EQELDLQTLYNIPDSHNFYCPTCNCCITKVIIRRDPP-------------------LPRS 180

Query: 181 TNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRI--ICSNCFSFLSPIGAWISS 240
             IP    RP+SP P  G  +   V   +    ++GCGRI  +CSNCFSFL+PIG W++S
Sbjct: 181 DVIPP-EIRPQSPGPDAGGTD---VRPRKFNPDDDGCGRIDVLCSNCFSFLAPIGKWMAS 240

Query: 241 QLGFGQKKPAI-SVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLG 300
              F  +KP I   K   GT+ T  AGPS  +  +P I+ E   S ++ IPP  G E  G
Sbjct: 241 L--FRPEKPQIPDEKAPPGTDTTGGAGPS-TTPGEPDIKDERPSSFIIPIPPGDGGERFG 300

Query: 301 Q--RGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360
           Q    GRS+EIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN
Sbjct: 301 QTVSRGRSLEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360

Query: 361 NISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGF 420
           NISGLKSNQLKKE N+   +A+VD YEEALGDR HYLLH+T AILSFLIFGLLPPLVYGF
Sbjct: 361 NISGLKSNQLKKEPNER-GEAKVDSYEEALGDRQHYLLHYTIAILSFLIFGLLPPLVYGF 420

Query: 421 SFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLS 480
           SFRDTDD DLKLAAVA SSLLCIALLAI KAY Q+A NWQ YAKTLVYYITLGFGASGLS
Sbjct: 421 SFRDTDDGDLKLAAVAASSLLCIALLAIAKAYAQRAQNWQAYAKTLVYYITLGFGASGLS 480

Query: 481 YLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           YLAGKEF+ LLEQ GWFK D   PTLLLPNMD A PTW SI
Sbjct: 481 YLAGKEFDKLLEQFGWFKGDPLPPTLLLPNMDFATPTWGSI 487

BLAST of PI0013404 vs. NCBI nr
Match: KAA0026135.1 (membrane protein of ER body-like protein [Cucumis melo var. makuwa] >TYK11595.1 membrane protein of ER body-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 471/517 (91.10%), Postives = 485/517 (93.81%), Query Frame = 0

Query: 1   MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGE 60
           MEDVVDLLPPPVEPVAGD KSREKHL RS SSSDSDEMYS GSPKEILKSGGKAIAPTGE
Sbjct: 1   MEDVVDLLPPPVEPVAGDGKSREKHLTRSCSSSDSDEMYSGGSPKEILKSGGKAIAPTGE 60

Query: 61  ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQE 120
           ADIIYIPHV+DKTLILNRNNSNKNINNNLTPPSTVS+LDIGGGN VTE+VYRKPF+DGQE
Sbjct: 61  ADIIYIPHVVDKTLILNRNNSNKNINNNLTPPSTVSVLDIGGGNNVTEIVYRKPFEDGQE 120

Query: 121 LDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEY-ELPPSTN 180
           LDLQTLY LPNSHNFFCPNCKSCITKVIILRD PSSP RSPRPQ GSKD EY +LP STN
Sbjct: 121 LDLQTLYKLPNSHNFFCPNCKSCITKVIILRDPPSSPSRSPRPQSGSKDREYDQLPSSTN 180

Query: 181 IPT-VTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRIICSNCFSFLSPIGAWISSQLG 240
           +PT +TTRP SPSPRP V++E GVDDGEIIS EEG GRIICSNCFSFL+PIGAWISSQLG
Sbjct: 181 VPTEITTRPWSPSPRPSVVQETGVDDGEIISSEEGGGRIICSNCFSFLTPIGAWISSQLG 240

Query: 241 FGQKKPAISVKIVGGTEATSHAGPSIISR-DQPFIEVEGGDSIVLTIPPVSGEETLGQRG 300
           FGQKKP I   IVGGTEATSHAGPSIISR DQPFIEVEGGDSIVLTIPPVSGEETLGQRG
Sbjct: 241 FGQKKPTIPADIVGGTEATSHAGPSIISRDDQPFIEVEGGDSIVLTIPPVSGEETLGQRG 300

Query: 301 GRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGL 360
           GR VEIMKSIVYGGLTEAITSLGIV SAASASTPTENIVALALANLITGLIVITNN+SGL
Sbjct: 301 GRRVEIMKSIVYGGLTEAITSLGIVASAASASTPTENIVALALANLITGLIVITNNLSGL 360

Query: 361 KSNQLKKESNQSDDQA-QVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD 420
           KSNQLKKESNQ+DD A QVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD
Sbjct: 361 KSNQLKKESNQNDDGAVQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRD 420

Query: 421 TDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAG 480
           TDD DLKLAAVAVSSLLCIALLAIVKAYVQKANN QEYAKTLVYY++LGFGASGLSYLAG
Sbjct: 421 TDDGDLKLAAVAVSSLLCIALLAIVKAYVQKANNLQEYAKTLVYYVSLGFGASGLSYLAG 480

Query: 481 KEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           KE NILLEQLGWFKKDQS PTLLLPNMDLAKPTW SI
Sbjct: 481 KEVNILLEQLGWFKKDQSTPTLLLPNMDLAKPTWGSI 517

BLAST of PI0013404 vs. NCBI nr
Match: XP_004149783.2 (membrane protein of ER body 2 [Cucumis sativus] >KGN44637.1 hypothetical protein Csa_016593 [Cucumis sativus])

HSP 1 Score: 885.2 bits (2286), Expect = 2.7e-253
Identity = 460/526 (87.45%), Postives = 481/526 (91.44%), Query Frame = 0

Query: 1   MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGE 60
           MEDVVD LPPPVEP+AGD K REKH+ RSGSSSDSD+MYS GSPKEILKSGGKAIAPTGE
Sbjct: 1   MEDVVDFLPPPVEPIAGDGKPREKHMTRSGSSSDSDDMYSGGSPKEILKSGGKAIAPTGE 60

Query: 61  ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQE 120
           ADIIYIPHV+DKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPF+DGQE
Sbjct: 61  ADIIYIPHVVDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFEDGQE 120

Query: 121 LDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEY-ELPPSTN 180
           LDLQTLY LPNSHNFFCPNCKSCITKVIILRD PSSP RSP+PQ GSKD EY +LP STN
Sbjct: 121 LDLQTLYKLPNSHNFFCPNCKSCITKVIILRDPPSSPSRSPQPQWGSKDREYDQLPSSTN 180

Query: 181 I-PTVTTRPRSPS--------PRPGVIEEAG-VDDGEIISREEGCGRIICSNCFSFLSPI 240
           I P +TTR RSPS        PRPGV+++ G +DD EIISREEG GRIICSNCFSFL+PI
Sbjct: 181 IPPEITTRARSPSPSPSPSPGPRPGVVQQTGALDDNEIISREEGGGRIICSNCFSFLTPI 240

Query: 241 GAWISSQLGFGQKKPAISVKIVGG-TEATSHAGPSIISRD-QPFIEVEGGDSIVLTIPPV 300
           GAWISSQLGFG KKP I   IVGG TE TS AGPSIISRD QPFI++EGG+SI  TIPP 
Sbjct: 241 GAWISSQLGFGPKKPTIPADIVGGITEGTSRAGPSIISRDGQPFIDIEGGESIAFTIPPA 300

Query: 301 SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL 360
           SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL
Sbjct: 301 SGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGL 360

Query: 361 IVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPP 420
           IVITNN+SGLKSNQLKKESNQSDD+AQVDPYEEALGDRHHYLLHFTTA+LSFLIFGLLPP
Sbjct: 361 IVITNNLSGLKSNQLKKESNQSDDRAQVDPYEEALGDRHHYLLHFTTAVLSFLIFGLLPP 420

Query: 421 LVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFG 480
           LVYGFSFRDTDD DLKLAAVA SSLLCIALLAI KAYVQKANNW+EYAKTLVYY+TLGFG
Sbjct: 421 LVYGFSFRDTDDGDLKLAAVAASSLLCIALLAIAKAYVQKANNWKEYAKTLVYYVTLGFG 480

Query: 481 ASGLSYLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           ASGLSYLAGKE NILLEQLGWFKKDQS PTLLLPNMDLAKPTW SI
Sbjct: 481 ASGLSYLAGKEVNILLEQLGWFKKDQSVPTLLLPNMDLAKPTWGSI 526

BLAST of PI0013404 vs. NCBI nr
Match: XP_038899240.1 (membrane protein of ER body 2-like [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.8e-229
Identity = 431/516 (83.53%), Postives = 459/516 (88.95%), Query Frame = 0

Query: 1   MEDVVDLLPPPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPTGE 60
           MEDV   +P  VEP+AGDTKSREKHL  + SSSDSDEMYS GSPKEILKSGGKAIAPTGE
Sbjct: 1   MEDVA-AMPAAVEPIAGDTKSREKHL-TTVSSSDSDEMYSDGSPKEILKSGGKAIAPTGE 60

Query: 61  ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQDGQE 120
           ADIIYIPHV+DKTLILNRNN++KN ++NLTPPSTVSLLDIGGGN+VTE++YRKPF+D +E
Sbjct: 61  ADIIYIPHVIDKTLILNRNNNDKN-HDNLTPPSTVSLLDIGGGNSVTEIIYRKPFEDERE 120

Query: 121 LDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYE-LPPSTN 180
           L LQTLY LPNSHNF+CPNCK+CITKVIILRD PSSP RSPRP   SKD  YE LP STN
Sbjct: 121 LYLQTLYELPNSHNFYCPNCKNCITKVIILRDTPSSPSRSPRPLSDSKDGAYEALPSSTN 180

Query: 181 IPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRIICSNCFSFLSPIGAWISSQLGF 240
           IPT  TRPRSP     VI E GVDDGEI S EEG GRIICSNCFSFL PIGAWISSQLGF
Sbjct: 181 IPT-ETRPRSPG--LDVIGEVGVDDGEIHSSEEGGGRIICSNCFSFLLPIGAWISSQLGF 240

Query: 241 GQKKPAISVKI-VGGTEATSHAG-PSIISRDQPFIEVEGGDSIVLTIPPVSGEETLGQRG 300
           GQKKPAI     +GGT  TSHAG P+IISR +P IEVEGGDSI +TIPPVS E+  G+RG
Sbjct: 241 GQKKPAIPTPTDIGGTAPTSHAGSPTIISR-EPVIEVEGGDSIAITIPPVSTEQASGRRG 300

Query: 301 GRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGL 360
           GRS+EIMKSIVYGGLTE ITSLGIVTSAASASTPTENIVALALANLITGLIVITNN+SGL
Sbjct: 301 GRSLEIMKSIVYGGLTEVITSLGIVTSAASASTPTENIVALALANLITGLIVITNNLSGL 360

Query: 361 KSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFSFRDT 420
           KSNQLKKESN+  DQA+VDPYEE LGDRHHYLLHFTTAILSFL+FGLLPPLVYGFSFRDT
Sbjct: 361 KSNQLKKESNEGVDQAEVDPYEETLGDRHHYLLHFTTAILSFLVFGLLPPLVYGFSFRDT 420

Query: 421 DDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAGK 480
           DD DLKLAAVAVSSLLCIALLAI KAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAGK
Sbjct: 421 DDGDLKLAAVAVSSLLCIALLAIAKAYVQKANNWQEYAKTLVYYITLGFGASGLSYLAGK 480

Query: 481 EFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           EFN+LLEQLGWFKKD +APTLLLPNMDLAKPTW SI
Sbjct: 481 EFNMLLEQLGWFKKDPNAPTLLLPNMDLAKPTWGSI 509

BLAST of PI0013404 vs. NCBI nr
Match: XP_022992032.1 (membrane protein of ER body 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 576.2 bits (1484), Expect = 2.7e-160
Identity = 341/521 (65.45%), Postives = 376/521 (72.17%), Query Frame = 0

Query: 1   MEDVVDLLP--PPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPT 60
           ME V+D L     VE  A D KSR+K L R+ SSSDSDEMYS GSPKEILK GGKAIAPT
Sbjct: 1   MEAVIDQLAAVEAVEAAADDRKSRKKQLSRT-SSSDSDEMYSGGSPKEILKLGGKAIAPT 60

Query: 61  GE-ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQD 120
           GE ADII+IPHV+DKTLILNR N     NN+  PP  VS +  GGG  V +  Y KP + 
Sbjct: 61  GEAADIIFIPHVIDKTLILNRGN-----NNSTPPPYDVSDIGGGGGGAVGD-GYPKPIEG 120

Query: 121 GQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPS 180
            QELDLQTLY LP+SHNF+CP CK CITKVII RD PS                    P 
Sbjct: 121 EQELDLQTLYKLPDSHNFYCPTCKCCITKVIIRRDPPS--------------------PR 180

Query: 181 TNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRI--ICSNCFSFLSPIGAWISS 240
           +++     RPRSP P  G      V D       +  GRI  +CSNCFSFL+PIG W+SS
Sbjct: 181 SDVTPTEIRPRSPGPDAG---RTDVRDDVFNPEGDHGGRIDVLCSNCFSFLAPIGKWMSS 240

Query: 241 QLGFGQKKPAI-SVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLG 300
              F ++KP   + K   GT+ TS AGPS  +  +P I+ EG  S V+ IPP  G E LG
Sbjct: 241 L--FRREKPQFPAEKAPPGTDTTSGAGPS-TTPGEPDIKDEGPPSFVIPIPPGDGGERLG 300

Query: 301 Q--RGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360
           Q   GGRS+EIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN
Sbjct: 301 QTVSGGRSLEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITN 360

Query: 361 NISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGF 420
           NISGLKSN+LKKE N+   +A+VD YEEALGDR HYLLHFT A+LSFLIFGLLPPLVYGF
Sbjct: 361 NISGLKSNKLKKEPNER-GEAKVDSYEEALGDRQHYLLHFTIAMLSFLIFGLLPPLVYGF 420

Query: 421 SFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLS 480
           SFR+TDD DLKLAAVA SSLLCIALLAI KAY Q+A NWQ YAKTLVYYITLGFGASGLS
Sbjct: 421 SFRNTDDGDLKLAAVAASSLLCIALLAIAKAYAQRARNWQAYAKTLVYYITLGFGASGLS 480

Query: 481 YLAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           YLAGKEF+ LLEQ GWFK D   PTL LPNMD A PTW SI
Sbjct: 481 YLAGKEFDKLLEQFGWFKGDPLPPTLSLPNMDFATPTWGSI 487

BLAST of PI0013404 vs. NCBI nr
Match: XP_022992033.1 (membrane protein of ER body-like protein isoform X2 [Cucurbita maxima])

HSP 1 Score: 575.1 bits (1481), Expect = 6.1e-160
Identity = 340/520 (65.38%), Postives = 375/520 (72.12%), Query Frame = 0

Query: 1   MEDVVDLLP--PPVEPVAGDTKSREKHLIRSGSSSDSDEMYSAGSPKEILKSGGKAIAPT 60
           ME V+D L     VE  A D KSR+K L R+ SSSDSDEMYS GSPKEILK GGKAIAPT
Sbjct: 1   MEAVIDQLAAVEAVEAAADDRKSRKKQLSRT-SSSDSDEMYSGGSPKEILKLGGKAIAPT 60

Query: 61  GE-ADIIYIPHVLDKTLILNRNNSNKNINNNLTPPSTVSLLDIGGGNTVTEVVYRKPFQD 120
           GE ADII+IPHV+DKTLILNR N     NN+  PP  VS +  GGG  V +  Y KP + 
Sbjct: 61  GEAADIIFIPHVIDKTLILNRGN-----NNSTPPPYDVSDIGGGGGGAVGD-GYPKPIEG 120

Query: 121 GQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPRPQLGSKDIEYELPPS 180
            QELDLQTLY LP+SHNF+CP CK CITKVII RD PS                    P 
Sbjct: 121 EQELDLQTLYKLPDSHNFYCPTCKCCITKVIIRRDPPS--------------------PR 180

Query: 181 TNIPTVTTRPRSPSPRPGVIEEAGVDDGEIISREEGCGRI--ICSNCFSFLSPIGAWISS 240
           +++     RPRSP P  G      V D       +  GRI  +CSNCFSFL+PIG W+SS
Sbjct: 181 SDVTPTEIRPRSPGPDAG---RTDVRDDVFNPEGDHGGRIDVLCSNCFSFLAPIGKWMSS 240

Query: 241 QLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIEVEGGDSIVLTIPPVSGEETLGQ 300
              F ++KP    +   GT+ TS AGPS  +  +P I+ EG  S V+ IPP  G E LGQ
Sbjct: 241 L--FRREKPQFPAE--KGTDTTSGAGPS-TTPGEPDIKDEGPPSFVIPIPPGDGGERLGQ 300

Query: 301 --RGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN 360
              GGRS+EIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN
Sbjct: 301 TVSGGRSLEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNN 360

Query: 361 ISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFS 420
           ISGLKSN+LKKE N+   +A+VD YEEALGDR HYLLHFT A+LSFLIFGLLPPLVYGFS
Sbjct: 361 ISGLKSNKLKKEPNER-GEAKVDSYEEALGDRQHYLLHFTIAMLSFLIFGLLPPLVYGFS 420

Query: 421 FRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSY 480
           FR+TDD DLKLAAVA SSLLCIALLAI KAY Q+A NWQ YAKTLVYYITLGFGASGLSY
Sbjct: 421 FRNTDDGDLKLAAVAASSLLCIALLAIAKAYAQRARNWQAYAKTLVYYITLGFGASGLSY 480

Query: 481 LAGKEFNILLEQLGWFKKDQSAPTLLLPNMDLAKPTWVSI 514
           LAGKEF+ LLEQ GWFK D   PTL LPNMD A PTW SI
Sbjct: 481 LAGKEFDKLLEQFGWFKGDPLPPTLSLPNMDFATPTWGSI 484

BLAST of PI0013404 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 152.5 bits (384), Expect = 9.0e-37
Identity = 123/408 (30.15%), Postives = 196/408 (48.04%), Query Frame = 0

Query: 103 GNTVTEVVYRKPFQDGQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPR 162
           GN   E   R      ++ +L+ L     ++  +CP+C +CIT+ ++L           +
Sbjct: 163 GNEDEEKNERSSSDSEEKSNLENLLATQENYELYCPSCSTCITRNVVL-----------K 222

Query: 163 PQLGSKDIEYELPPSTNIPTVTTRPRSP-------SPRPGVIEEAGVDDGEIISREEGCG 222
            +   K +   L    +IP V   P  P       SP    + E  ++D     +E+  G
Sbjct: 223 KRKRGKHVNSSLDLKPDIPVV--EPDEPSDIEEMESPVKVYVPETRIED----DQEDKEG 282

Query: 223 RII-CSNC-FSFLSPIGAWISSQLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIE 282
            I  C  C   +   +G     QL + + KP                   +    + +I+
Sbjct: 283 TIFTCLVCDLKYFIRLGTKF-LQLDYIRGKP-------------------VEKSVEEYID 342

Query: 283 VEGGDSIVLTIPPVSGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTE 342
           V    +   + P +  +   G+R   ++E++KS VYGGLTE ITSLG+V+SA+++ + T 
Sbjct: 343 VRKSINTTQSPPQIQPD---GER--FAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 402

Query: 343 NIVALALANLITGLIVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFT 402
           NI+ALA+ANL  GLIV+  N   L+        N SD +   D YEE LG R    +H  
Sbjct: 403 NILALAVANLAGGLIVLAQNFQDLR--------NSSDQEK--DRYEELLGRRTKSRIHIL 462

Query: 403 TAILSFLIFGLLPPLVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANN--- 462
            A++S++ FGL+PPLVY FSF +T   + KL +V + SL+C+ LL  +K YV+K  N   
Sbjct: 463 VAVMSYIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCG 518

Query: 463 -WQEYAKTLVYYITLGFGASGLSYLAGKEFNILLEQLGWFKKDQSAPT 498
             + Y K+  YY ++   + G+SY+ G      +E+L     DQ + T
Sbjct: 523 STKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKLSLVGLDQISIT 518

BLAST of PI0013404 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 152.5 bits (384), Expect = 9.0e-37
Identity = 123/408 (30.15%), Postives = 196/408 (48.04%), Query Frame = 0

Query: 103 GNTVTEVVYRKPFQDGQELDLQTLYNLPNSHNFFCPNCKSCITKVIILRDAPSSPPRSPR 162
           GN   E   R      ++ +L+ L     ++  +CP+C +CIT+ ++L           +
Sbjct: 179 GNEDEEKNERSSSDSEEKSNLENLLATQENYELYCPSCSTCITRNVVL-----------K 238

Query: 163 PQLGSKDIEYELPPSTNIPTVTTRPRSP-------SPRPGVIEEAGVDDGEIISREEGCG 222
            +   K +   L    +IP V   P  P       SP    + E  ++D     +E+  G
Sbjct: 239 KRKRGKHVNSSLDLKPDIPVV--EPDEPSDIEEMESPVKVYVPETRIED----DQEDKEG 298

Query: 223 RII-CSNC-FSFLSPIGAWISSQLGFGQKKPAISVKIVGGTEATSHAGPSIISRDQPFIE 282
            I  C  C   +   +G     QL + + KP                   +    + +I+
Sbjct: 299 TIFTCLVCDLKYFIRLGTKF-LQLDYIRGKP-------------------VEKSVEEYID 358

Query: 283 VEGGDSIVLTIPPVSGEETLGQRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTE 342
           V    +   + P +  +   G+R   ++E++KS VYGGLTE ITSLG+V+SA+++ + T 
Sbjct: 359 VRKSINTTQSPPQIQPD---GER--FAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 418

Query: 343 NIVALALANLITGLIVITNNISGLKSNQLKKESNQSDDQAQVDPYEEALGDRHHYLLHFT 402
           NI+ALA+ANL  GLIV+  N   L+        N SD +   D YEE LG R    +H  
Sbjct: 419 NILALAVANLAGGLIVLAQNFQDLR--------NSSDQEK--DRYEELLGRRTKSRIHIL 478

Query: 403 TAILSFLIFGLLPPLVYGFSFRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANN--- 462
            A++S++ FGL+PPLVY FSF +T   + KL +V + SL+C+ LL  +K YV+K  N   
Sbjct: 479 VAVMSYIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCG 534

Query: 463 -WQEYAKTLVYYITLGFGASGLSYLAGKEFNILLEQLGWFKKDQSAPT 498
             + Y K+  YY ++   + G+SY+ G      +E+L     DQ + T
Sbjct: 539 STKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKLSLVGLDQISIT 534

BLAST of PI0013404 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 152.1 bits (383), Expect = 1.2e-36
Identity = 89/195 (45.64%), Postives = 128/195 (65.64%), Query Frame = 0

Query: 298 GRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNISGL 357
           GR +EI+KSIVYGGL EAITSLG+++SAA +     NI+ L LANL+ GLI+I +N+  L
Sbjct: 540 GRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQEL 599

Query: 358 KSNQ---LKKESNQSDDQAQVD-PYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVYGFS 417
           +  +      E NQ++ + + +  Y+  LG R ++ LH T AILSF+I G+LPP+VY FS
Sbjct: 600 REEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFS 659

Query: 418 FRDTDDSDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGASGLSY 477
           F +  + D K+A+V  +SL CI LLAI KA+V+       Y K+++YY ++    SG+SY
Sbjct: 660 FSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRG--SYLKSILYYGSIAVSVSGISY 719

Query: 478 LAGKEFNILLEQLGW 489
           + G     LLE+ GW
Sbjct: 720 VVGNFLEQLLEKHGW 732


HSP 2 Score: 43.5 bits (101), Expect = 5.9e-04
Identity = 15/37 (40.54%), Postives = 26/37 (70.27%), Query Frame = 0

Query: 115 FQDGQELDLQTLYNLPNSHNFFCPNCKSCITKVIILR 152
           F+D +  D++ + +   +H+ +CPNC SCITK +IL+
Sbjct: 192 FEDVEYHDVENMMDKQETHDLYCPNCDSCITKKVILK 228

BLAST of PI0013404 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 149.8 bits (377), Expect = 5.9e-36
Identity = 93/201 (46.27%), Postives = 129/201 (64.18%), Query Frame = 0

Query: 295 QRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNI 354
           ++GG  VEI+KSIVYGGLTE+ITSL  VTSAA++   T N++AL +ANL +GL++  +++
Sbjct: 405 RKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSL 464

Query: 355 SGLKSNQLKKESNQSDD----QAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVY 414
             L + + +K++N  D     + + D YEE LG R +  +H   AI SF+IFGL+PPLVY
Sbjct: 465 QELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVY 524

Query: 415 GFSFRDTDD--SDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGA 474
           GFSFR   +   + K+ AV   SLLCI LL+I KAYV K    ++Y KTL  Y T    A
Sbjct: 525 GFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK---RDYVKTLFRYTTTATTA 584

Query: 475 SGLSYLAGKEFNILLEQLGWF 490
           SG S   G   +  LE+ G++
Sbjct: 585 SGFSQFVGYLVSQWLEKSGFY 602

BLAST of PI0013404 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 149.8 bits (377), Expect = 5.9e-36
Identity = 93/201 (46.27%), Postives = 129/201 (64.18%), Query Frame = 0

Query: 295 QRGGRSVEIMKSIVYGGLTEAITSLGIVTSAASASTPTENIVALALANLITGLIVITNNI 354
           ++GG  VEI+KSIVYGGLTE+ITSL  VTSAA++   T N++AL +ANL +GL++  +++
Sbjct: 390 RKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVHSL 449

Query: 355 SGLKSNQLKKESNQSDD----QAQVDPYEEALGDRHHYLLHFTTAILSFLIFGLLPPLVY 414
             L + + +K++N  D     + + D YEE LG R +  +H   AI SF+IFGL+PPLVY
Sbjct: 450 QELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPLVY 509

Query: 415 GFSFRDTDD--SDLKLAAVAVSSLLCIALLAIVKAYVQKANNWQEYAKTLVYYITLGFGA 474
           GFSFR   +   + K+ AV   SLLCI LL+I KAYV K    ++Y KTL  Y T    A
Sbjct: 510 GFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKK---RDYVKTLFRYTTTATTA 569

Query: 475 SGLSYLAGKEFNILLEQLGWF 490
           SG S   G   +  LE+ G++
Sbjct: 570 SGFSQFVGYLVSQWLEKSGFY 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KFS71.3e-3530.15Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1[more]
Q8LPT31.7e-3545.64Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL... [more]
Q8W4P88.3e-3546.27Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CJ515.6e-26091.10Membrane protein of ER body-like protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0K8E61.3e-25387.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G352500 PE=3 SV=1[more]
A0A6J1JNK91.3e-16065.45membrane protein of ER body 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JXX62.9e-16065.38membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1GPB56.5e-16065.45membrane protein of ER body 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
KAA0026135.11.1e-25991.10membrane protein of ER body-like protein [Cucumis melo var. makuwa] >TYK11595.1 ... [more]
XP_004149783.22.7e-25387.45membrane protein of ER body 2 [Cucumis sativus] >KGN44637.1 hypothetical protein... [more]
XP_038899240.12.8e-22983.53membrane protein of ER body 2-like [Benincasa hispida][more]
XP_022992032.12.7e-16065.45membrane protein of ER body 1-like isoform X1 [Cucurbita maxima][more]
XP_022992033.16.1e-16065.38membrane protein of ER body-like protein isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G24290.29.0e-3730.15Vacuolar iron transporter (VIT) family protein [more]
AT5G24290.19.0e-3730.15Vacuolar iron transporter (VIT) family protein [more]
AT4G27870.11.2e-3645.64Vacuolar iron transporter (VIT) family protein [more]
AT4G27860.15.9e-3646.27vacuolar iron transporter (VIT) family protein [more]
AT4G27860.25.9e-3646.27vacuolar iron transporter (VIT) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..44
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..198
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..31
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 260..512
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 30..244
NoneNo IPR availableCDDcd01059CCC1_likecoord: 304..479
e-value: 4.68084E-8
score: 50.3028
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 30..244
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 260..512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013404.1PI0013404.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030026 cellular manganese ion homeostasis
biological_process GO:0006880 intracellular sequestering of iron ion
biological_process GO:0034755 iron ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
cellular_component GO:0010168 ER body
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005381 iron ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity