PI0013299 (gene) Melon (PI 482460) v1

Overview
NamePI0013299
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCalcium-binding EF hand family protein
Locationchr04: 8485653 .. 8486306 (-)
RNA-Seq ExpressionPI0013299
SyntenyPI0013299
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAACTCAGTCCCCAACCAAAAATCTATATCCATGTTCTTTAGATTAGAGTACTATTTAATCACAATGCAAACCCGGCTAACAGACGTCAAAAGCGTGCAGCTTGACCCGTCACCGCTCGTGTTTGTCGATCGGCTATCAATTCTACCAGTTTTATTCAACTTTGCAGACAGAATAAATGAGCTGATCAGATCTCTATCTTGGTCAATTCTTCAATCACAGTCCAAAGAGAAAGCTCTATCACAAAGTAATTGCTCTTCACAATCTGATGATCAAATCATCACTCAACAAAATTGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAAAAAGGGATGATGATGATGATCAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAG

mRNA sequence

ATTAACTCAGTCCCCAACCAAAAATCTATATCCATGTTCTTTAGATTAGAGTACTATTTAATCACAATGCAAACCCGGCTAACAGACGTCAAAAGCGTGCAGCTTGACCCGTCACCGCTCGTGTTTGTCGATCGGCTATCAATTCTACCAGTTTTATTCAACTTTGCAGACAGAATAAATGAGCTGATCAGATCTCTATCTTGGTCAATTCTTCAATCACAGTCCAAAGAGAAAGCTCTATCACAAAGTAATTGCTCTTCACAATCTGATGATCAAATCATCACTCAACAAAATTGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAAAAAGGGATGATGATGATGATCAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAG

Coding sequence (CDS)

ATGTTCTTTAGATTAGAGTACTATTTAATCACAATGCAAACCCGGCTAACAGACGTCAAAAGCGTGCAGCTTGACCCGTCACCGCTCGTGTTTGTCGATCGGCTATCAATTCTACCAGTTTTATTCAACTTTGCAGACAGAATAAATGAGCTGATCAGATCTCTATCTTGGTCAATTCTTCAATCACAGTCCAAAGAGAAAGCTCTATCACAAAGTAATTGCTCTTCACAATCTGATGATCAAATCATCACTCAACAAAATTGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAAAAAGGGATGATGATGATGATCAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAG

Protein sequence

MFFRLEYYLITMQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS
Homology
BLAST of PI0013299 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.5e-19
Identity = 61/144 (42.36%), Postives = 83/144 (57.64%), Query Frame = 0

Query: 61  QSQSKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGE 120
           + Q +     + +     DD      +  +SREE   VM  L LF+    +DD  QE   
Sbjct: 54  KDQQETLTKQEDDDDDDDDDDDDDDDDIDISREEAEMVMRSLGLFY----NDDQLQEQYS 113

Query: 121 SDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTM 180
           + E +  +FEE E SLEE+KQ F+VFD N+DGFID  EL  VL++L   +G ++ +C  M
Sbjct: 114 AKE-VSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVM 173

Query: 181 IARFDLNNDGKIDFHEFVKFMEVA 205
           I   D N DGKIDF+EFVKFME +
Sbjct: 174 IRSLDGNKDGKIDFNEFVKFMETS 192

BLAST of PI0013299 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.1e-17
Identity = 69/199 (34.67%), Postives = 107/199 (53.77%), Query Frame = 0

Query: 7   YYLITMQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQ--- 66
           Y  ITM++ L+  K       PL  +    ++   F +     +L  S  W ++Q Q   
Sbjct: 7   YSFITMKSSLSKCKQSSSLSFPLFGLINFFLIG-FFRWVS-FAQLFFSRFWPLVQHQQCV 66

Query: 67  SKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGESDE 126
           S++K+      +S   ++     +  + RE+V  VM+ L L   ++ +    Q    S +
Sbjct: 67  SEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQ---YSSK 126

Query: 127 IIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIAR 186
            +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++L   +G  + +C+ MI  
Sbjct: 127 EVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRS 186

Query: 187 FDLNNDGKIDFHEFVKFME 203
           FD + DG+IDF+ FVKFME
Sbjct: 187 FDGSKDGRIDFYGFVKFME 200

BLAST of PI0013299 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.4e-11
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 0

Query: 118 IGESDEIIRGMFEENEPSL---------EELKQTFNVFDRNRDGFIDEHELFIVLSLLES 177
           I +S+ +   + EE E  L         E +K+ F +FD N+DGFIDE+EL  VLSLL  
Sbjct: 87  IDDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGY 146

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           ++   + +C+ M+  +D N DGKIDF+EFVK +E + S
Sbjct: 147 DECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

BLAST of PI0013299 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 9.7e-11
Identity = 38/110 (34.55%), Postives = 67/110 (60.91%), Query Frame = 0

Query: 93  EEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDG 152
           +E+  ++EK+++      + D   +I E  E+ + + +E +   E++K+ FNVFD+N DG
Sbjct: 40  KELTQMIEKIDV------NGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDG 99

Query: 153 FIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 203
           FI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 FITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

BLAST of PI0013299 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 36/89 (40.45%), Postives = 57/89 (64.04%), Query Frame = 0

Query: 114 DDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 173
           D +E GE  + I  + +E+E   E++K+ FNVFDRN DGFI   EL  VLS L   +G  
Sbjct: 58  DIEEFGELYKTIM-VEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKT 117

Query: 174 IHDCKTMIARFDLNNDGKIDFHEFVKFME 203
           + +C+ MI + D++ DG++++ EF + M+
Sbjct: 118 LEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

BLAST of PI0013299 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.3e-92
Identity = 185/210 (88.10%), Postives = 194/210 (92.38%), Query Frame = 0

Query: 1   MFFRLEYYLITMQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSIL 60
           MFFRLEYYLITM+TRLTD+KSVQLDPSP VFVDR+SILP+LFNFADRI EL+RSL W IL
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QSQSKEKALSQSNCSSQSDDQII--TQQNC-SMSREEVRFVMEKLELFWREKRD-DDDDQ 120
           Q  SKEKALSQ NCSS+S DQII  TQQNC SMSREEVRFVMEKLELFWRE+RD    D+
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 EIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180
            IGESDE+IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           CKTMIARFDLNNDGKIDFHEFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

BLAST of PI0013299 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.6e-85
Identity = 178/200 (89.00%), Postives = 188/200 (94.00%), Query Frame = 0

Query: 12  MQTRLTD-VKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLS-WSILQSQ-SKEKA 71
           MQT++T+ VKSVQLDPSPLVFVD +S LP+LFNFA RI+ELIRSL  WSILQSQ SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDQII--TQQNCSMSREEVRFVMEKLELFWREKR-DDDDDQEIGESDEII 131
           LSQSNCSSQSDD+II  TQQNCSMSREEVRFVMEKLELFWRE+  DDDDD+EIG SDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVAL 206
           LNNDGKIDFHEFVKFMEVAL
Sbjct: 181 LNNDGKIDFHEFVKFMEVAL 200

BLAST of PI0013299 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.3e-45
Identity = 116/181 (64.09%), Postives = 136/181 (75.14%), Query Frame = 0

Query: 26  PSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQSNCSSQSDDQIITQ 85
           P PLVF+DR SI      FAD I++L+ SL++S  QS  KEKA  QS  +SQS+     +
Sbjct: 21  PPPLVFIDRASI------FADTIHDLV-SLAFSFFQSPPKEKAAPQSR-ASQSEG----K 80

Query: 86  QNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEPSLEELKQTFNV 145
           ++  MSREEV+ VMEKLEL+  E+ DD+ D+  G   E +RGMFEENEPSLEELKQTFNV
Sbjct: 81  EDWLMSREEVKSVMEKLELYCNEEGDDEIDECFGR--EEVRGMFEENEPSLEELKQTFNV 140

Query: 146 FDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 205
           FDRNRDGFID HEL  VL LL SN  IFIHDCK+MIARFD NND KIDF+EFVKFMEV+L
Sbjct: 141 FDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVKFMEVSL 187

Query: 206 S 207
           S
Sbjct: 201 S 187

BLAST of PI0013299 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 5.3e-44
Identity = 116/192 (60.42%), Postives = 140/192 (72.92%), Query Frame = 0

Query: 21  SVQLDPS------PLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQSNC 80
           ++QL PS      PLVF+DR SI      FA  I++L+ SL++S  QS  K+KA  QS  
Sbjct: 9   NLQLIPSTTTTTPPLVFIDRASI------FAGTIHDLV-SLAFSFFQSPPKDKAAPQSR- 68

Query: 81  SSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEP 140
           +SQS+    ++++  MSREEV+ VMEKLEL+  E+ DD+ D+  G  +  +RGMFEENEP
Sbjct: 69  ASQSE----SKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDE--VRGMFEENEP 128

Query: 141 SLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDF 200
           SLEELKQTFNVFDRNRDGFID  EL  VL LL SN  IFIHDCK+MIARFD NND KIDF
Sbjct: 129 SLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDF 186

Query: 201 HEFVKFMEVALS 207
           +EFVKFMEVALS
Sbjct: 189 NEFVKFMEVALS 186

BLAST of PI0013299 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 2.3e-39
Identity = 101/178 (56.74%), Postives = 124/178 (69.66%), Query Frame = 0

Query: 29  LVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQSNCSSQSDDQIITQQNC 88
           L+ VDR+SI  +LF+F DRI++LI S  WS+  S  KEKA  Q N     + +    +N 
Sbjct: 16  LLLVDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKASPQGN---SLESEATQHKNS 75

Query: 89  SMSREEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDR 148
           S+S+EEV+FVMEKLE F      D++ +E     E I  +F E+EPSLEELKQTFNVFD+
Sbjct: 76  SISKEEVKFVMEKLEFFC----SDEEIEESWLGGEEIGAIFYESEPSLEELKQTFNVFDK 135

Query: 149 NRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           NRDG ID  EL  VL LL  N+G+FIHDC  MIARFD N DGKIDF+EFVKFME+ALS
Sbjct: 136 NRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFMEIALS 185

BLAST of PI0013299 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 348.2 bits (892), Expect = 4.8e-92
Identity = 185/210 (88.10%), Postives = 194/210 (92.38%), Query Frame = 0

Query: 1   MFFRLEYYLITMQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSIL 60
           MFFRLEYYLITM+TRLTD+KSVQLDPSP VFVDR+SILP+LFNFADRI EL+RSL W IL
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QSQSKEKALSQSNCSSQSDDQII--TQQNC-SMSREEVRFVMEKLELFWREKRD-DDDDQ 120
           Q  SKEKALSQ NCSS+S DQII  TQQNC SMSREEVRFVMEKLELFWRE+RD    D+
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 EIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180
            IGESDE+IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           CKTMIARFDLNNDGKIDFHEFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

BLAST of PI0013299 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 327.4 bits (838), Expect = 8.8e-86
Identity = 174/199 (87.44%), Postives = 183/199 (91.96%), Query Frame = 0

Query: 12  MQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQ 71
           M+TRLTD+KSVQLDPSP VFVDR+SILP+LFNFADRI EL+RSL W ILQ  SKEKALSQ
Sbjct: 1   METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 60

Query: 72  SNCSSQSDDQII--TQQNC-SMSREEVRFVMEKLELFWREKRD-DDDDQEIGESDEIIRG 131
            NCSS+S DQII  TQQNC SMSREEVRFVMEKLELFWRE+RD    D+ IGESDE+IRG
Sbjct: 61  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 120

Query: 132 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 191
           MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN
Sbjct: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180

Query: 192 NDGKIDFHEFVKFMEVALS 207
           NDGKIDFHEFVKFMEVALS
Sbjct: 181 NDGKIDFHEFVKFMEVALS 199

BLAST of PI0013299 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 324.3 bits (830), Expect = 7.5e-85
Identity = 178/200 (89.00%), Postives = 188/200 (94.00%), Query Frame = 0

Query: 12  MQTRLTD-VKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLS-WSILQSQ-SKEKA 71
           MQT++T+ VKSVQLDPSPLVFVD +S LP+LFNFA RI+ELIRSL  WSILQSQ SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDQII--TQQNCSMSREEVRFVMEKLELFWREKR-DDDDDQEIGESDEII 131
           LSQSNCSSQSDD+II  TQQNCSMSREEVRFVMEKLELFWRE+  DDDDD+EIG SDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVAL 206
           LNNDGKIDFHEFVKFMEVAL
Sbjct: 181 LNNDGKIDFHEFVKFMEVAL 200

BLAST of PI0013299 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 255.8 bits (652), Expect = 3.3e-64
Identity = 148/198 (74.75%), Postives = 161/198 (81.31%), Query Frame = 0

Query: 12  MQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQSKEKALSQ 71
           MQ RL DV +VQL P+PLVFVDR SILP+LF FADRI++LI S+  S  QS SKEKA  Q
Sbjct: 1   MQNRLPDV-NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPLSFFQSPSKEKASPQ 60

Query: 72  SNCSSQSDDQIITQQ-NCSMSREEVRFVMEKLELFWREKRDDDDDQEIGE--SDEIIRGM 131
           SNCSSQSDDQ+ITQQ NC MSREEVRFVM+KLELF  +   +D D+EIGE    E +  M
Sbjct: 61  SNCSSQSDDQVITQQKNCLMSREEVRFVMDKLELFCSK---NDGDEEIGECFGGEELTAM 120

Query: 132 FEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNN 191
           FEENEPSLEELKQTFNVFDRNRDGFID  EL IVL LL+SN GIFIHDCK MIARFD NN
Sbjct: 121 FEENEPSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFDHNN 180

Query: 192 DGKIDFHEFVKFMEVALS 207
           DGKIDF EFVKFMEVALS
Sbjct: 181 DGKIDFTEFVKFMEVALS 193

BLAST of PI0013299 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197.2 bits (500), Expect = 1.4e-46
Identity = 128/212 (60.38%), Postives = 151/212 (71.23%), Query Frame = 0

Query: 1   MFFRLEYYLITMQTRLTDVKSVQLDPS------PLVFVDRLSILPVLFNFADRINELIRS 60
           MFF+LE YL+TM      + ++QL PS      PLVF+DR SI      FAD I++LI S
Sbjct: 1   MFFKLE-YLLTMHNPQPTI-NLQLIPSTTTTTPPLVFIDRASI------FADTIHDLI-S 60

Query: 61  LSWSILQSQSKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDD 120
           L +S  QS  KEKA  QS  +SQS+     +++  MSREEV+ VMEKLEL+  E+ DD+ 
Sbjct: 61  LVFSFFQSPPKEKAAPQSR-ASQSEG----KEDWLMSREEVKSVMEKLELYCNEEEDDEI 120

Query: 121 DQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFI 180
           D+  G  +  +RGMFEENEPSLEELKQTFNVFDRNRDGFID  EL  VL LL SN  IFI
Sbjct: 121 DECFGRDE--VRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFI 180

Query: 181 HDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           HDCK+MIARFD NND KIDF+EFVKFMEVALS
Sbjct: 181 HDCKSMIARFDHNNDQKIDFNEFVKFMEVALS 196

BLAST of PI0013299 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 61/144 (42.36%), Postives = 83/144 (57.64%), Query Frame = 0

Query: 61  QSQSKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGE 120
           + Q +     + +     DD      +  +SREE   VM  L LF+    +DD  QE   
Sbjct: 54  KDQQETLTKQEDDDDDDDDDDDDDDDDIDISREEAEMVMRSLGLFY----NDDQLQEQYS 113

Query: 121 SDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTM 180
           + E +  +FEE E SLEE+KQ F+VFD N+DGFID  EL  VL++L   +G ++ +C  M
Sbjct: 114 AKE-VSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVM 173

Query: 181 IARFDLNNDGKIDFHEFVKFMEVA 205
           I   D N DGKIDF+EFVKFME +
Sbjct: 174 IRSLDGNKDGKIDFNEFVKFMETS 192

BLAST of PI0013299 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.9e-18
Identity = 69/199 (34.67%), Postives = 107/199 (53.77%), Query Frame = 0

Query: 7   YYLITMQTRLTDVKSVQLDPSPLVFVDRLSILPVLFNFADRINELIRSLSWSILQSQ--- 66
           Y  ITM++ L+  K       PL  +    ++   F +     +L  S  W ++Q Q   
Sbjct: 7   YSFITMKSSLSKCKQSSSLSFPLFGLINFFLIG-FFRWVS-FAQLFFSRFWPLVQHQQCV 66

Query: 67  SKEKALSQSNCSSQSDDQIITQQNCSMSREEVRFVMEKLELFWREKRDDDDDQEIGESDE 126
           S++K+      +S   ++     +  + RE+V  VM+ L L   ++ +    Q    S +
Sbjct: 67  SEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQ---YSSK 126

Query: 127 IIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIAR 186
            +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++L   +G  + +C+ MI  
Sbjct: 127 EVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRS 186

Query: 187 FDLNNDGKIDFHEFVKFME 203
           FD + DG+IDF+ FVKFME
Sbjct: 187 FDGSKDGRIDFYGFVKFME 200

BLAST of PI0013299 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.1e-12
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 0

Query: 118 IGESDEIIRGMFEENEPSL---------EELKQTFNVFDRNRDGFIDEHELFIVLSLLES 177
           I +S+ +   + EE E  L         E +K+ F +FD N+DGFIDE+EL  VLSLL  
Sbjct: 87  IDDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGY 146

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 207
           ++   + +C+ M+  +D N DGKIDF+EFVK +E + S
Sbjct: 147 DECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

BLAST of PI0013299 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 68.6 bits (166), Expect = 6.9e-12
Identity = 38/110 (34.55%), Postives = 67/110 (60.91%), Query Frame = 0

Query: 93  EEVRFVMEKLELFWREKRDDDDDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDG 152
           +E+  ++EK+++      + D   +I E  E+ + + +E +   E++K+ FNVFD+N DG
Sbjct: 40  KELTQMIEKIDV------NGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDG 99

Query: 153 FIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 203
           FI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 FITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

BLAST of PI0013299 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 65.1 bits (157), Expect = 7.6e-11
Identity = 36/89 (40.45%), Postives = 57/89 (64.04%), Query Frame = 0

Query: 114 DDQEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 173
           D +E GE  + I  + +E+E   E++K+ FNVFDRN DGFI   EL  VLS L   +G  
Sbjct: 58  DIEEFGELYKTIM-VEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKT 117

Query: 174 IHDCKTMIARFDLNNDGKIDFHEFVKFME 203
           + +C+ MI + D++ DG++++ EF + M+
Sbjct: 118 LEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MBG51.5e-1942.36Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ... [more]
Q93Z274.1e-1734.67Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ... [more]
Q9SN894.4e-1141.84Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ... [more]
Q9LNE79.7e-1134.55Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1[more]
Q9ZR021.1e-0940.45Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LHQ52.3e-9288.10Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501... [more]
A0A1S3B8I13.6e-8589.00probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P... [more]
A0A6J1J5Q61.3e-4564.09probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819... [more]
A0A6J1F8545.3e-4460.42probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1EIK82.3e-3956.74probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
KGN59556.14.8e-9288.10hypothetical protein Csa_001548 [Cucumis sativus][more]
XP_004147503.18.8e-8687.44probable calcium-binding protein CML45 [Cucumis sativus][more]
XP_008443616.17.5e-8589.00PREDICTED: probable calcium-binding protein CML30 [Cucumis melo][more]
XP_038904514.13.3e-6474.75probable calcium-binding protein CML45 [Benincasa hispida][more]
XP_023528818.11.4e-4660.38probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G29000.11.1e-2042.36Calcium-binding EF-hand family protein [more]
AT5G39670.12.9e-1834.67Calcium-binding EF-hand family protein [more]
AT3G47480.13.1e-1241.84Calcium-binding EF-hand family protein [more]
AT1G05990.16.9e-1234.55EF hand calcium-binding protein family [more]
AT4G03290.17.6e-1140.45EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 138..166
e-value: 2.4E-5
score: 33.8
coord: 176..204
e-value: 8.7E-4
score: 28.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 137..202
e-value: 1.7E-9
score: 37.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 172..206
score: 11.33333
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 134..169
score: 13.537066
IPR002048EF-hand domainCDDcd00051EFhcoord: 138..201
e-value: 1.43713E-13
score: 61.0245
NoneNo IPR availableGENE3D1.10.238.10coord: 62..205
e-value: 1.5E-18
score: 69.4
NoneNo IPR availablePANTHERPTHR10891:SF745CALCIUM-BINDING PROTEIN CML46-RELATEDcoord: 6..202
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 6..202
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 185..197
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 147..159
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 131..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013299.1PI0013299.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding