PI0013250 (gene) Melon (PI 482460) v1

Overview
NamePI0013250
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCytochrome P450 71A1-like
Locationchr04: 15917674 .. 15919970 (-)
RNA-Seq ExpressionPI0013250
SyntenyPI0013250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATTTTTACCTAATTTTCATTCCTATTTATACCTCTTCAAACCTCATCACATTTCCACCATCACAAATAACAACAAATCCCCATTTCTACCATCAAATTCACCATGGAAATGAAATTTCCTTATGTAGCTGTTGCTTGGGTAGCCACTTTGGCCATCATCTTCCTCTCCCGCCGTCTCCGCCGCCGCAAACTCAACCTCCCACCCGGACCTAAACCATGGCCCCTAATCGGAAACCTCGACTTAATTGGCTCTCTGCCTCACCAATCCATTCATCAACTCTCCAAAAAATACGGCCCATAATGCATCTTCGTTTCGGATCATTCCCTGTCGTCGTTGGATCCTCCGTCGAAATGGCAAAGATTTTCCTCAAAACACAAGATCTCAATTTCGTGTCGCGCCCGAAAACCGCGGCGGGAAAATACACAACTTATAACTATTCCGACATTACTTGGTCCCAATACGGCCCTTATTGGCGGCAAGCTCGTAAGATGTGTTTGATGGAGCTTTTTAGTGCAAGAAGACTTGATTCTTATGAGTATATACGTAAGGAAGAAATGAAAGCTCTGCTTAGAGAAATTTACAAGTCTTGTGGTGAAGTAATTAAGCTTAAGGATTATTTGTCTACGGTGAGTTTGAATGTGATAAGTAGGATGGTATTGGGAAAAAAATATACGGATGAATCAGAAAATGGGATAGTTAGTCCAGATGAGTTTAAGAAAATGTTGGACGAGCTGTTTTTGCTGAGTGGTGTGTTGAATATTGGGGATTCAATTCCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGAATGAAGGTTTTGAGTAAGAAATTTGATAGGTTTCTTGAACATGTGTTGGATGAACATAATGAAAGAAGAAAGGGAGTTGAGAATTATGTGGCTAAAGATATGGTGGATGTTTTGTTGCAATTGGCTGATGATCCTGATCTTGAAGTTAAACTTGAAAGACATGGAGTCAAGGCTTTTACTCAGGTAAGTTGTTTTCCTTTACAGTCTATACCTTGTTTAATCATAGCCACTTCTTAAATTATTCTTTTTTCACACAAATATATATATATATATATATATATATATTATATTAGTAATCCATTAAAATGTTAGACAAAATTTGAGACATAAAATACTATTATTCACTTCACCTGGAACAAACACTAAGACCCACGCCACTTCCAGGACCCAATTTCTTAAAAAGAAATTCAAATGAATTTCTACTTTAGTAGTAGTTTAAACTTAATTAAAAGGGCAATATGTTCACTATCAAAAGAATAGAGGTATTTTTTATACAGTGAACAAAACTCTAACACTAACTTTGTAATTTAGCCTAATATTAACTACATTTCCCAAATAAATGCATGTTGAGAAGGGATTAATTTTAAAATGACAAACTATTTTCAAGTGTTTCCTATGAATACTTTTAAACTCACAAATATTTACTGTTTTTTTTTTTTCAAAACATGTAGGATCTTATAGCAGGTGGAACCGAGAGTTCAGCAGTGACAGTAGAATGGGCAATGTCAGAGCTATTAAAAAAGCCAGAGATATTCAACAAGGCAAGAGAAGAACTCGATAGAGTAATTGGAAGAGAAAGATGGGTAGAAGAGAAAGACATTGTAAATTTACCTTACATCGATGCAATTGCCAAAGAAACAATGAGACTACACCCAGTGGCTCCAATGTTAGTGCCAAGAATGGCTCGAGAGGATTCTCAAATTGCAGGGTATGACATAGCCAAAGGCACTAGAGTGTTTGTGAATGTATGGACCATTGGTAGAGACCCAACAGTGTGGGAAAATCCACTTGAATTTAAGCCAGAAAGGTTCATGGGAAAGAACATTGATGTGAAAGGTCAAGACTTCGAGCTTTTGCCATTTGGGTCAGGAAGAAGGATGTGTCCTGGATATAATCTTGGCTTGAAGGTTATTCAATCAAGTTTAGCTAATTTGTTGCATGGGTTTACCTGGAAGTTGAGTGGAGATATGAAGAATGAAGATTTGAATATGGATGAAGTTTTTGGTCTTTCAACTCCAAAGAAATTTCCTCTTGATGTTGTGGCAGAGCCAAGGCTTTCTCCTTCTCTTTACTCCATTTAATCATTAAGACTTGAAGGGAATTGCTCTTTTTTCCATTTAGAGATCATTTAAGTAGTTTCTATGTGTTATAGCTTGATTATTGTTATACTAAGTCTTAAGTCAATCAGAATAATTCAAATGAAAGTGATACCGTAGGAGGTGAGAAATAATAATGATGGTCAAATGTGAGTTTGAATTTA

mRNA sequence

CCATTTTTACCTAATTTTCATTCCTATTTATACCTCTTCAAACCTCATCACATTTCCACCATCACAAATAACAACAAATCCCCATTTCTACCATCAAATTCACCATGGAAATGAAATTTCCTTATGTAGCTGTTGCTTGGGTAGCCACTTTGGCCATCATCTTCCTCTCCCGCCGTCTCCGCCGCCGCAAACTCAACCTCCCACCCGGACCTAAACCATGGCCCCTAATCGGAAACCTCGACTTAATTGGCTCTCTGCCTCACCAATCCATTCATCAACTCTCCAAAAAATACGGCCCATAATGCATCTTCGTTTCGGATCATTCCCTGTCGTCGTTGGATCCTCCGTCGAAATGGCAAAGATTTTCCTCAAAACACAAGATCTCAATTTCGTGTCGCGCCCGAAAACCGCGGCGGGAAAATACACAACTTATAACTATTCCGACATTACTTGGTCCCAATACGGCCCTTATTGGCGGCAAGCTCGTAAGATGTGTTTGATGGAGCTTTTTAGTGCAAGAAGACTTGATTCTTATGAGTATATACGTAAGGAAGAAATGAAAGCTCTGCTTAGAGAAATTTACAAGTCTTGTGGTGAAGTAATTAAGCTTAAGGATTATTTGTCTACGGTGAGTTTGAATGTGATAAGTAGGATGGTATTGGGAAAAAAATATACGGATGAATCAGAAAATGGGATAGTTAGTCCAGATGAGTTTAAGAAAATGTTGGACGAGCTGTTTTTGCTGAGTGGTGTGTTGAATATTGGGGATTCAATTCCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGAATGAAGGTTTTGAGTAAGAAATTTGATAGGTTTCTTGAACATGTGTTGGATGAACATAATGAAAGAAGAAAGGGAGTTGAGAATTATGTGGCTAAAGATATGGTGGATGTTTTGTTGCAATTGGCTGATGATCCTGATCTTGAAGTTAAACTTGAAAGACATGGAGTCAAGGCTTTTACTCAGGATCTTATAGCAGGTGGAACCGAGAGTTCAGCAGTGACAGTAGAATGGGCAATGTCAGAGCTATTAAAAAAGCCAGAGATATTCAACAAGGCAAGAGAAGAACTCGATAGAGTAATTGGAAGAGAAAGATGGGTAGAAGAGAAAGACATTGTAAATTTACCTTACATCGATGCAATTGCCAAAGAAACAATGAGACTACACCCAGTGGCTCCAATGTTAGTGCCAAGAATGGCTCGAGAGGATTCTCAAATTGCAGGGTATGACATAGCCAAAGGCACTAGAGTGTTTGTGAATGTATGGACCATTGGTAGAGACCCAACAGTGTGGGAAAATCCACTTGAATTTAAGCCAGAAAGGTTCATGGGAAAGAACATTGATGTGAAAGGTCAAGACTTCGAGCTTTTGCCATTTGGGTCAGGAAGAAGGATGTGTCCTGGATATAATCTTGGCTTGAAGGTTATTCAATCAAGTTTAGCTAATTTGTTGCATGGGTTTACCTGGAAGTTGAGTGGAGATATGAAGAATGAAGATTTGAATATGGATGAAGTTTTTGGTCTTTCAACTCCAAAGAAATTTCCTCTTGATGTTGTGGCAGAGCCAAGGCTTTCTCCTTCTCTTTACTCCATTTAATCATTAAGACTTGAAGGGAATTGCTCTTTTTTCCATTTAGAGATCATTTAAGTAGTTTCTATGTGTTATAGCTTGATTATTGTTATACTAAGTCTTAAGTCAATCAGAATAATTCAAATGAAAGTGATACCGTAGGAGGTGAGAAATAATAATGATGGTCAAATGTGAGTTTGAATTTA

Coding sequence (CDS)

ATGGCCCCTAATCGGAAACCTCGACTTAATTGGCTCTCTGCCTCACCAATCCATTCATCAACTCTCCAAAAAATACGGCCCATAATGCATCTTCGTTTCGGATCATTCCCTGTCGTCGTTGGATCCTCCGTCGAAATGGCAAAGATTTTCCTCAAAACACAAGATCTCAATTTCGTGTCGCGCCCGAAAACCGCGGCGGGAAAATACACAACTTATAACTATTCCGACATTACTTGGTCCCAATACGGCCCTTATTGGCGGCAAGCTCGTAAGATGTGTTTGATGGAGCTTTTTAGTGCAAGAAGACTTGATTCTTATGAGTATATACGTAAGGAAGAAATGAAAGCTCTGCTTAGAGAAATTTACAAGTCTTGTGGTGAAGTAATTAAGCTTAAGGATTATTTGTCTACGGTGAGTTTGAATGTGATAAGTAGGATGGTATTGGGAAAAAAATATACGGATGAATCAGAAAATGGGATAGTTAGTCCAGATGAGTTTAAGAAAATGTTGGACGAGCTGTTTTTGCTGAGTGGTGTGTTGAATATTGGGGATTCAATTCCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGAATGAAGGTTTTGAGTAAGAAATTTGATAGGTTTCTTGAACATGTGTTGGATGAACATAATGAAAGAAGAAAGGGAGTTGAGAATTATGTGGCTAAAGATATGGTGGATGTTTTGTTGCAATTGGCTGATGATCCTGATCTTGAAGTTAAACTTGAAAGACATGGAGTCAAGGCTTTTACTCAGGATCTTATAGCAGGTGGAACCGAGAGTTCAGCAGTGACAGTAGAATGGGCAATGTCAGAGCTATTAAAAAAGCCAGAGATATTCAACAAGGCAAGAGAAGAACTCGATAGAGTAATTGGAAGAGAAAGATGGGTAGAAGAGAAAGACATTGTAAATTTACCTTACATCGATGCAATTGCCAAAGAAACAATGAGACTACACCCAGTGGCTCCAATGTTAGTGCCAAGAATGGCTCGAGAGGATTCTCAAATTGCAGGGTATGACATAGCCAAAGGCACTAGAGTGTTTGTGAATGTATGGACCATTGGTAGAGACCCAACAGTGTGGGAAAATCCACTTGAATTTAAGCCAGAAAGGTTCATGGGAAAGAACATTGATGTGAAAGGTCAAGACTTCGAGCTTTTGCCATTTGGGTCAGGAAGAAGGATGTGTCCTGGATATAATCTTGGCTTGAAGGTTATTCAATCAAGTTTAGCTAATTTGTTGCATGGGTTTACCTGGAAGTTGAGTGGAGATATGAAGAATGAAGATTTGAATATGGATGAAGTTTTTGGTCTTTCAACTCCAAAGAAATTTCCTCTTGATGTTGTGGCAGAGCCAAGGCTTTCTCCTTCTCTTTACTCCATTTAA

Protein sequence

MAPNRKPRLNWLSASPIHSSTLQKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNVISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKVLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLYSI
Homology
BLAST of PI0013250 vs. ExPASy Swiss-Prot
Match: A0A1D6F9Y9 (Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.2e-127
Identity = 230/464 (49.57%), Postives = 319/464 (68.75%), Query Frame = 0

Query: 27  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 86
           P+MHLR GS+  VV SS + A++ LK  DL F  RP+TAAG+  +Y Y  I  + YG YW
Sbjct: 67  PLMHLRLGSYHAVVASSADTARLVLKVHDLAFADRPRTAAGEVASYGYLGIVHTPYGAYW 126

Query: 87  RQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGE-VIKLKDYLSTVSLNVISR 146
           R ARK+C  ELFSARR+DS+E +R +EM+AL R +++  G   + ++++++  +L  I R
Sbjct: 127 RMARKLCATELFSARRVDSFERVRAQEMRALARGLFECAGRGAVAVREHVAGATLRNILR 186

Query: 147 MVLGKKYTDESENGIVSPDEFKKMLDELFLLSG-VLNIGDSIPWIDFLDLQGYVKRMKVL 206
           M +G+K++     G    + F++ LDE F ++G V N+G+ +PW+ +LDLQG V+RMK L
Sbjct: 187 MAVGEKWS--GCYGSPEGEAFRRTLDEAFAVTGAVSNVGEWVPWLGWLDLQGCVRRMKRL 246

Query: 207 SKKFDRFLEHVLDEHNER-------RKGVENYVA-KDMVDVLLQLADDPD------LEVK 266
             ++DRF E +LD+H+ +       R G  +  A  D+VDVLL+L ++ +         +
Sbjct: 247 RAQYDRFFEQILDDHDHKKTRRERGRPGAGDSAADDDLVDVLLRLVEEDEDRPETTEASR 306

Query: 267 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKD 326
           L R GVKAF QD++AGGTESSAVT+EWAMSELL++P+    A  ELDRVIG  RWV E+D
Sbjct: 307 LTRDGVKAFVQDIVAGGTESSAVTIEWAMSELLRRPDALRAATAELDRVIGHGRWVTERD 366

Query: 327 IVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVW 386
           + +LPYIDA+ KETMRLHPV P+LVP  ARE + +AGYD+  G RV VNVW I RDP  W
Sbjct: 367 LPDLPYIDAVVKETMRLHPVGPLLVPHHAREHTVVAGYDVPAGARVLVNVWAIARDPASW 426

Query: 387 -ENPLEFKPERFM----GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHG 446
            + P  F+PERF+    G ++DV+G  FELLPFG+GRRMCP + L +K++ + +ANL+HG
Sbjct: 427 PDAPDAFRPERFLNGSSGASVDVRGAHFELLPFGAGRRMCPAHGLAMKLVTAGVANLVHG 486

Query: 447 FTWKLSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLYS 470
           F W+L   M  ED++M+E+FGLST +K PL  VAEPRL   LY+
Sbjct: 487 FAWRLPDGMAPEDVSMEELFGLSTRRKVPLVAVAEPRLPAHLYT 528

BLAST of PI0013250 vs. ExPASy Swiss-Prot
Match: A0A1D6HSP4 (Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.6e-120
Identity = 219/458 (47.82%), Postives = 309/458 (67.47%), Query Frame = 0

Query: 27  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 86
           P+MHLR GS+  VV SS + A++ L+T D     RP TAAG+ T+Y Y  I  +  G YW
Sbjct: 73  PLMHLRLGSYHTVVASSADAARLVLRTHDSALADRPDTAAGEITSYGYLGIVHTPRGAYW 132

Query: 87  RQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCG--EVIKLKDYLSTVSLNVIS 146
           R AR++C  ELFSARR++S++ +R +EM+AL R ++        + ++++++  ++  I 
Sbjct: 133 RMARRLCATELFSARRVESFQDVRAQEMRALARGLFGCAAGRRAVAVREHVAGATMRNIL 192

Query: 147 RMVLGKKYTDESENGIVSPDEFKKMLDELFLLSG-VLNIGDSIPWIDFLDLQGYVKRMKV 206
           RM +G+K++     G    + F++ LDE F  +G V N+G+ +PW+ +LD+QG+ ++MK 
Sbjct: 193 RMAVGEKWS--GCYGSPEGEAFRRSLDEAFAATGAVSNVGEWVPWLGWLDVQGFKRKMKR 252

Query: 207 LSKKFDRFLEHVLDEHNERRKGVE----NYVAKDMVDVLLQLAD-----DPDLEVKLERH 266
           L    D F E +L +H ERR+  +     +VA D+VDVLLQL++     + + E +L R 
Sbjct: 253 LHDLHDHFYEKILVDHEERRRLAQASGGEFVATDLVDVLLQLSEESTKLESESEARLPRD 312

Query: 267 GVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNL 326
           GVKA  QD+IAGGTESSAVT+EWAM+ELL+ PE   KA +ELDRV+G  RWV E+D+  L
Sbjct: 313 GVKALIQDIIAGGTESSAVTIEWAMAELLRHPEAMAKATDELDRVVGSGRWVAERDLPEL 372

Query: 327 PYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVW-ENP 386
            YIDA+ KET+RLHPV P+LVP  ARE + +AGYD+  G RV VN W I RDP  W + P
Sbjct: 373 HYIDAVVKETLRLHPVGPLLVPHYARERTVVAGYDVPAGARVLVNAWAIARDPASWPDAP 432

Query: 387 LEFKPERFMG--KNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLS 446
             F+PERF+G    +DV+G  FELLPFGSGRR+CP Y+L +K++ + +ANL+HGF W+L 
Sbjct: 433 DAFQPERFLGAAAAVDVRGAHFELLPFGSGRRICPAYDLAMKLVAAGVANLVHGFAWRLP 492

Query: 447 GDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLYS 470
             +  ED++M+E  GLST +K PL  VAEPRL   LYS
Sbjct: 493 DGVAAEDVSMEEHVGLSTRRKVPLFAVAEPRLPVHLYS 528

BLAST of PI0013250 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 1.1e-110
Identity = 204/452 (45.13%), Postives = 289/452 (63.94%), Query Frame = 0

Query: 20  STLQKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITW 79
           S   ++ P++ L  G  P ++ S+ E+A+  LKT DL F SRP T A +   Y+ +D+ +
Sbjct: 57  SLANELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAF 116

Query: 80  SQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSC--GEVIKLKDYLST 139
           S YG YWRQ RK+C++EL S +R++SY  IR+EE+  ++  I +SC  GE + L + L  
Sbjct: 117 SPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLL 176

Query: 140 VSLNVISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIP---WIDFLDL 199
           +S   I+R+  GKKY  E E      ++F  +  EL  L G   +GD  P   W+D   L
Sbjct: 177 LSSGTITRVAFGKKYEGEEER----KNKFADLATELTTLMGAFFVGDYFPSFAWVDV--L 236

Query: 200 QGYVKRMKVLSKKFDRFLEHVLDEHNERRK--GVENYVAKDMVDVLLQLADDPDLEVKLE 259
            G   R+K    + D F++HV+D+H   RK  G +    KD+VDVLL L  D  L V L 
Sbjct: 237 TGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLN 296

Query: 260 RHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIV 319
           R+ +KA   D+ +GGT+++AVT+EWAM+EL+K P++  KA++E+ RV+G++  VEE+D+ 
Sbjct: 297 RNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLH 356

Query: 320 NLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWEN 379
            L Y+  I KET+RLHPVAP+LVPR +  D  I GY I   TRVF+N W IGRDP  WEN
Sbjct: 357 QLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWEN 416

Query: 380 PLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSG 439
             EF PERF+  ++D KGQDF+L+PFG+GRR CPG   G+  ++ SLANLL+ F W+L G
Sbjct: 417 AEEFLPERFVNNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPG 476

Query: 440 DMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLS 465
           D+  EDL+M E  G++   KFPL +VA+  LS
Sbjct: 477 DLTKEDLDMSEAVGITVHMKFPLQLVAKRHLS 502

BLAST of PI0013250 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.3e-105
Identity = 194/461 (42.08%), Postives = 290/461 (62.91%), Query Frame = 0

Query: 18  HSST---LQKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNY 77
           H ST    Q   P+M+L+ G   VVV +S  +A  FLKT D NF SRP  +  ++  YNY
Sbjct: 54  HQSTAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNY 113

Query: 78  SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDY 137
            D+ ++ YGP WR  RK+C + LFS + LD + ++R++E+K L R +  +  + +KL   
Sbjct: 114 QDLVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQL 173

Query: 138 LSTVSLNVISRMVLGKK-YTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLD 197
           L+  + N ++R++LGK+ + D S +      EFK M+ E+ +++GV NIGD IP +++LD
Sbjct: 174 LNVCTTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLD 233

Query: 198 LQGYVKRMKVLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLA-DDPDLE-VKL 257
           +QG   +MK L  +FD FL  +L+EH    KG      KD++  L+ L  DD D +  KL
Sbjct: 234 IQGVAAKMKKLHARFDAFLTDILEEH----KGKIFGEMKDLLSTLISLKNDDADNDGGKL 293

Query: 258 ERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDI 317
               +KA   +L   GT++S+ TVEWA++EL++ P+I  +A++E+D+V+GR+R V E D+
Sbjct: 294 TDTEIKALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDL 353

Query: 318 VNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWE 377
             L Y++AI KET RLHP  P+ +PR+A E  +I GY I KG+ + +NVW I RDP  W 
Sbjct: 354 AQLTYLEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWA 413

Query: 378 NPLEFKPERFM----GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFT 437
           +PLEF+PERF+       +DV+G DFE++PFG+GRR+C G NLG++++Q  +A L+H F 
Sbjct: 414 DPLEFRPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFN 473

Query: 438 WKLSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLY 469
           W L      E LNM+E +GL+  +  PL V   PRL    Y
Sbjct: 474 WDLVSGQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of PI0013250 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.5e-104
Identity = 185/449 (41.20%), Postives = 285/449 (63.47%), Query Frame = 0

Query: 27  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 86
           P+  LR GS  VVV SS  +A  FL+T D+NF +RP  +  ++  YNY D+ ++ YGP W
Sbjct: 66  PLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRW 125

Query: 87  RQARKMCLMELFSARRLDSYEYIRKEEMKALLREI--YKSCGEVIKLKDYLSTVSLNVIS 146
           R  RK+C + LFS + LD    +R+ E+  L+R +  +   G ++ L   L+  + N ++
Sbjct: 126 RMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALA 185

Query: 147 RMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKVL 206
           R +LG++   + +  +   DEFK+M+ EL  L+GV N+GD +P + +LDLQG V +MK L
Sbjct: 186 RAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRL 245

Query: 207 SKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLAD-DPDLEVKLERHGVKAFTQDL 266
            +++D FL+ V++E+    K      + D++ VL++L + D + E+KL    +KA   +L
Sbjct: 246 HRRYDAFLDRVIEENQANAK------SGDLLSVLIRLKEADAEGEIKLNNTDIKALLLNL 305

Query: 267 IAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAKE 326
              GT++S+ TVEW ++EL++ P+I  K + ELD VIGR+R V E D+ NLPY+ A+ KE
Sbjct: 306 FTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKE 365

Query: 327 TMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM- 386
           T RLHP  P+ +PRMA E+  + GY I K   + VNVW+IGRD  VW +PLEF+P RF+ 
Sbjct: 366 TFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLP 425

Query: 387 ---GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDL 446
               +++DVKG DFE++PFG+GRR+C G +LGL+++Q   A ++H + W L    + + L
Sbjct: 426 GGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKL 485

Query: 447 NMDEVFGLSTPKKFPLDVVAEPRLSPSLY 469
           +M+E +GL+  +  PL V   PRLS   Y
Sbjct: 486 DMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of PI0013250 vs. ExPASy TrEMBL
Match: A0A5D3D8H9 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1267G00050 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.0e-252
Identity = 434/448 (96.88%), Postives = 439/448 (97.99%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 61  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 120

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSARRLDSYEYIRKEEM ALLREIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 121 GPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNV 180

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 181 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 240

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHNERRKGVE+YVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 241 ALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 300

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDI+NLPYIDAIAK
Sbjct: 301 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIINLPYIDAIAK 360

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDI KGTRVFVNVWTIGRDPTVWENPLEFKPERFM
Sbjct: 361 ETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 420

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GKNIDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD
Sbjct: 421 GKNIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 480

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVAEPRLS SLY I
Sbjct: 481 EVFGLSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of PI0013250 vs. ExPASy TrEMBL
Match: A0A1S3CCA6 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103499163 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.7e-252
Identity = 433/448 (96.65%), Postives = 439/448 (97.99%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 61  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 120

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSARRLDSYEYIRKEEM ALLREIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 121 GPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNV 180

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 181 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 240

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHNERRKGVE+YVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 241 ALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 300

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIG+ERWVEEKDI+NLPYIDAIAK
Sbjct: 301 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKDIINLPYIDAIAK 360

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDI KGTRVFVNVWTIGRDPTVWENPLEFKPERFM
Sbjct: 361 ETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 420

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GKNIDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD
Sbjct: 421 GKNIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 480

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVAEPRLS SLY I
Sbjct: 481 EVFGLSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of PI0013250 vs. ExPASy TrEMBL
Match: A0A6J1GXG6 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.9e-241
Identity = 411/448 (91.74%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKT DLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 66  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQY 125

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSA+RLDSYEYIR+EEM ALLR++Y+SCG+VIK+KDYLSTVSLNV
Sbjct: 126 GPYWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYRSCGDVIKVKDYLSTVSLNV 185

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 186 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 245

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHN RR+GV++YVA+DMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 246 ALSKKFDRFLEHVLDEHNARREGVKDYVAQDMVDVLLQLADDPDLEVKLERHGVKAFTQD 305

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELL+KPEI NKAREELDRVIG+ERWVEEKDI +LPYIDAIAK
Sbjct: 306 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDIASLPYIDAIAK 365

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDIAKGTRV VNVWTIGRDP VWENP EF PERFM
Sbjct: 366 ETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPAVWENPNEFSPERFM 425

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GK+IDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTW L GDMK EDLNMD
Sbjct: 426 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWSLGGDMKKEDLNMD 485

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVA+PRL P LYSI
Sbjct: 486 EVFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of PI0013250 vs. ExPASy TrEMBL
Match: A0A6J1IRR8 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 406/448 (90.62%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKT DLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 66  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQY 125

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSA+RLDSYEYIR+EEM ALLR++YKSCGEV+++KDYLSTVSLNV
Sbjct: 126 GPYWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYKSCGEVMRVKDYLSTVSLNV 185

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 186 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 245

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHN RR+GV++YVAKDMVDVLLQLADDP LEVKLERHGVKAFTQD
Sbjct: 246 ALSKKFDRFLEHVLDEHNARREGVKDYVAKDMVDVLLQLADDPHLEVKLERHGVKAFTQD 305

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELL+KPEI NKAREELDRVIG+ERWVEEKD+ +LPYIDAIAK
Sbjct: 306 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDMASLPYIDAIAK 365

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDI KGTRV VNVWTIGRDP VWENP EF PERFM
Sbjct: 366 ETMRLHPVAPMLVPRMCREDCQIAGYDIVKGTRVLVNVWTIGRDPAVWENPSEFSPERFM 425

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GK+IDVKGQDFELLPFGSGRRMCPGY+LGLKVIQ+SLANLLHGFTW L  DMK EDLNMD
Sbjct: 426 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQTSLANLLHGFTWSLGEDMKKEDLNMD 485

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           E+FGLSTPKKFPLDVVA+PRL P LYSI
Sbjct: 486 EIFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of PI0013250 vs. ExPASy TrEMBL
Match: A0A5D3C5I8 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00890 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 409/449 (91.09%), Postives = 426/449 (94.88%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 65  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 124

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSARRLDSYEYIRKEEM  L+REIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 125 GPYWRQARKMCLMELFSARRLDSYEYIRKEEMNGLVREIYKSCGEVIKVKDYLSTVSLNV 184

Query: 143 ISRMVLGKKYTDE-SENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRM 202
           ISRMVLGKKYTDE SENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRM
Sbjct: 185 ISRMVLGKKYTDESSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRM 244

Query: 203 KVLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQ 262
           K LSKKFDRFLEHVLDEHNERRKGV+NYVAKDMVDVLLQLADDP+LEVKLERHGVKAFTQ
Sbjct: 245 KALSKKFDRFLEHVLDEHNERRKGVQNYVAKDMVDVLLQLADDPNLEVKLERHGVKAFTQ 304

Query: 263 DLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIA 322
           DLIAGGTESSAVTVEWA+SELLK PEIFNKA EEL+ VIG+ERWVEEKDIVNLPYI+AIA
Sbjct: 305 DLIAGGTESSAVTVEWAISELLKNPEIFNKAIEELNEVIGKERWVEEKDIVNLPYINAIA 364

Query: 323 KETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERF 382
           KETMRLHPVAPMLVPRMARED QIAGYDIAKGTRV VNVWTIGRDPTVW NP  F P+RF
Sbjct: 365 KETMRLHPVAPMLVPRMAREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWRNPHAFDPDRF 424

Query: 383 MGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNM 442
           M  +IDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGF WKL GDM+ EDLNM
Sbjct: 425 MESSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFNWKLPGDMEKEDLNM 484

Query: 443 DEVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           +E+FGLSTPKK+PLD VAEPRL P LYS+
Sbjct: 485 EEIFGLSTPKKYPLDAVAEPRLPPHLYSL 513

BLAST of PI0013250 vs. NCBI nr
Match: KAA0053469.1 (cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYK19885.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 880.6 bits (2274), Expect = 6.2e-252
Identity = 434/448 (96.88%), Postives = 439/448 (97.99%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 61  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 120

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSARRLDSYEYIRKEEM ALLREIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 121 GPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNV 180

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 181 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 240

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHNERRKGVE+YVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 241 ALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 300

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDI+NLPYIDAIAK
Sbjct: 301 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIINLPYIDAIAK 360

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDI KGTRVFVNVWTIGRDPTVWENPLEFKPERFM
Sbjct: 361 ETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 420

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GKNIDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD
Sbjct: 421 GKNIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 480

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVAEPRLS SLY I
Sbjct: 481 EVFGLSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of PI0013250 vs. NCBI nr
Match: XP_008460308.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 433/448 (96.65%), Postives = 439/448 (97.99%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 61  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 120

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSARRLDSYEYIRKEEM ALLREIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 121 GPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNV 180

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 181 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 240

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHNERRKGVE+YVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 241 ALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 300

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIG+ERWVEEKDI+NLPYIDAIAK
Sbjct: 301 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKDIINLPYIDAIAK 360

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDI KGTRVFVNVWTIGRDPTVWENPLEFKPERFM
Sbjct: 361 ETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 420

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GKNIDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD
Sbjct: 421 GKNIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 480

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVAEPRLS SLY I
Sbjct: 481 EVFGLSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of PI0013250 vs. NCBI nr
Match: XP_004144428.3 (trimethyltridecatetraene synthase [Cucumis sativus] >KGN58597.2 hypothetical protein Csa_002014 [Cucumis sativus])

HSP 1 Score: 867.1 bits (2239), Expect = 7.1e-248
Identity = 429/448 (95.76%), Postives = 437/448 (97.54%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTY+YS+ITWSQY
Sbjct: 61  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYDYSNITWSQY 120

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSA+RLDSYEYIRKEEM  LL EIYKSCGEVIK+KDYLSTVSLNV
Sbjct: 121 GPYWRQARKMCLMELFSAKRLDSYEYIRKEEMNGLLGEIYKSCGEVIKVKDYLSTVSLNV 180

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTD SENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 181 ISRMVLGKKYTDGSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 240

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLE VLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHG+KAFTQD
Sbjct: 241 ALSKKFDRFLERVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGIKAFTQD 300

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWA+SELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK
Sbjct: 301 LIAGGTESSAVTVEWAISELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 360

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRV VNVWTIGRDPTVWE+PLEFKPERFM
Sbjct: 361 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVLVNVWTIGRDPTVWEDPLEFKPERFM 420

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMK EDLNMD
Sbjct: 421 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKIEDLNMD 480

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVAEPRLS SLYSI
Sbjct: 481 EVFGLSTPKKFPLDVVAEPRLSSSLYSI 508

BLAST of PI0013250 vs. NCBI nr
Match: XP_038876425.1 (trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 856.3 bits (2211), Expect = 1.2e-244
Identity = 428/477 (89.73%), Postives = 440/477 (92.24%), Query Frame = 0

Query: 1   MAPNRKP-----RLNWLSASPIHS--STLQKIRPIMHLRFGSFPVVVGSSVEMAKIFLKT 60
           + P  KP      LN + + P  S     +K  PIMHLRFGSFPVVVGSSVEMAKIFLKT
Sbjct: 70  LPPGPKPWPLIGNLNLIGSLPHQSIHELSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKT 129

Query: 61  QDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEE 120
            DL FVSRPKTAAGKYTTYNYS+ITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEE
Sbjct: 130 HDLTFVSRPKTAAGKYTTYNYSNITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEE 189

Query: 121 MKALLREIYKSCGEVIKLKDYLSTVSLNVISRMVLGKKYTDESENGIVSPDEFKKMLDEL 180
           M ALL++IYKSCG+VIKLKDYLSTVSLNVISRMVLGKKYTDESEN IVSPDEFKKMLDEL
Sbjct: 190 MNALLKDIYKSCGQVIKLKDYLSTVSLNVISRMVLGKKYTDESENAIVSPDEFKKMLDEL 249

Query: 181 FLLSGVLNIGDSIPWIDFLDLQGYVKRMKVLSKKFDRFLEHVLDEHNERRKGVENYVAKD 240
           FLLSGVLNIGDSIPWIDFLDLQGYVKRMK LSKKFDRFLEHVLDEHNERRKGVENYVAKD
Sbjct: 250 FLLSGVLNIGDSIPWIDFLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVENYVAKD 309

Query: 241 MVDVLLQLADDPDLEVKLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAR 300
           MVDVLLQLADDP LEVKLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEI NKAR
Sbjct: 310 MVDVLLQLADDPHLEVKLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEILNKAR 369

Query: 301 EELDRVIGRERWVEEKDIVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKG 360
           EELDRVIG+ERWVEEKD+VNLPYIDAIAKETMRLHPVAPMLVPRM+RED QIAGYDIAKG
Sbjct: 370 EELDRVIGKERWVEEKDVVNLPYIDAIAKETMRLHPVAPMLVPRMSREDCQIAGYDIAKG 429

Query: 361 TRVFVNVWTIGRDPTVWENPLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLK 420
           TRV VNVWTIGRDPTVWENP EF PERFMGK IDVKGQDFELLPFGSGRRMCPGY+LGLK
Sbjct: 430 TRVLVNVWTIGRDPTVWENPYEFNPERFMGKTIDVKGQDFELLPFGSGRRMCPGYSLGLK 489

Query: 421 VIQSSLANLLHGFTWKLSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           VIQSSLANLLHGF WKL GDMK EDLNMDEVFGLSTPKKFPLDVVAEPRLSP LYSI
Sbjct: 490 VIQSSLANLLHGFYWKLGGDMKKEDLNMDEVFGLSTPKKFPLDVVAEPRLSPFLYSI 546

BLAST of PI0013250 vs. NCBI nr
Match: XP_022956310.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 842.8 bits (2176), Expect = 1.4e-240
Identity = 411/448 (91.74%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           +K  PIMHLRFGSFPVVVGSSVEMAKIFLKT DLNFVSRPKTAAGKYTTYNYSDITWSQY
Sbjct: 66  KKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQY 125

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNV 142
           GPYWRQARKMCLMELFSA+RLDSYEYIR+EEM ALLR++Y+SCG+VIK+KDYLSTVSLNV
Sbjct: 126 GPYWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYRSCGDVIKVKDYLSTVSLNV 185

Query: 143 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 202
           ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK
Sbjct: 186 ISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMK 245

Query: 203 VLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQD 262
            LSKKFDRFLEHVLDEHN RR+GV++YVA+DMVDVLLQLADDPDLEVKLERHGVKAFTQD
Sbjct: 246 ALSKKFDRFLEHVLDEHNARREGVKDYVAQDMVDVLLQLADDPDLEVKLERHGVKAFTQD 305

Query: 263 LIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAK 322
           LIAGGTESSAVTVEWAMSELL+KPEI NKAREELDRVIG+ERWVEEKDI +LPYIDAIAK
Sbjct: 306 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDIASLPYIDAIAK 365

Query: 323 ETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM 382
           ETMRLHPVAPMLVPRM RED QIAGYDIAKGTRV VNVWTIGRDP VWENP EF PERFM
Sbjct: 366 ETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPAVWENPNEFSPERFM 425

Query: 383 GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 442
           GK+IDVKGQDFELLPFGSGRRMCPGY+LGLKVIQSSLANLLHGFTW L GDMK EDLNMD
Sbjct: 426 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWSLGGDMKKEDLNMD 485

Query: 443 EVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           EVFGLSTPKKFPLDVVA+PRL P LYSI
Sbjct: 486 EVFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of PI0013250 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.9e-103
Identity = 189/451 (41.91%), Postives = 281/451 (62.31%), Query Frame = 0

Query: 27  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 86
           PI+HLR G   VVV +S  +A+ FLK  D NF SRP  +  K+  YNY D+ ++ YG  W
Sbjct: 66  PILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRW 125

Query: 87  RQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGEVIKLKDYLSTVSLNVISRM 146
           R  RK+  + LFSA+ L+ ++++R+EE+  L RE+ +   + + L   ++   +N + R 
Sbjct: 126 RLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGRE 185

Query: 147 VLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKVLSK 206
           ++G++      +     DEF+ M+ E+  L+GV NIGD +P +D+LDLQG   +MK L K
Sbjct: 186 MIGRRLFGADADH--KADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHK 245

Query: 207 KFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLE---VKLERHGVKAFTQDL 266
           +FD FL  +L EH    +  ++    DM+  L+ L    DL+     L    +KA   ++
Sbjct: 246 RFDAFLSSILKEHEMNGQDQKH---TDMLSTLISL-KGTDLDGDGGSLTDTEIKALLLNM 305

Query: 267 IAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAKE 326
              GT++SA TV+WA++EL++ P+I  KA+EELD V+GR+R V E DI  LPY+ A+ KE
Sbjct: 306 FTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKE 365

Query: 327 TMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFM- 386
             RLHP  P+ +P +A E  +I GY I KG+ +  N+W I RDP  W +PL FKPERF+ 
Sbjct: 366 NFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLP 425

Query: 387 ---GKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDL 446
                 +DVKG DFEL+PFG+GRR+C G +LGL+ IQ   A L+ GF W+L+G +  E L
Sbjct: 426 GGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKL 485

Query: 447 NMDEVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           NM+E +GL+  +  PL V  +PRL+P++Y +
Sbjct: 486 NMEESYGLTLQRAVPLVVHPKPRLAPNVYGL 510

BLAST of PI0013250 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 332.4 bits (851), Expect = 5.8e-91
Identity = 177/444 (39.86%), Postives = 263/444 (59.23%), Query Frame = 0

Query: 27  PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 86
           PI  L  GS   +V +S  +A+  LK QD+NF +R     G+  TY   DI W+ YG  W
Sbjct: 75  PIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEW 134

Query: 87  RQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGE--VIKLKDYLSTVSLNVIS 146
           RQ RK+C+++L S + LDS+  +R++E++   R +Y+   +   +K+ D L    +N+  
Sbjct: 135 RQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTM 194

Query: 147 RMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKVL 206
            M+ G     E    + +  EFK ++ E+  L    N+ D  PW+   DLQG VKRM V 
Sbjct: 195 NMLWGGSVKAEEMESVGT--EFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRMGVC 254

Query: 207 SKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLAD-DPDLEVKLERHGVKAFTQDL 266
           +++ D  L+  +++    R G ++   KD +  L++L D + D EV +  + VKA   D+
Sbjct: 255 ARELDAVLDRAIEQMKPLR-GRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKALLTDM 314

Query: 267 IAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNLPYIDAIAKE 326
           + GGT++S  T+E+AM+EL+  PE+  +A+EELD V+G++  VEE  I  LPYI AI KE
Sbjct: 315 VVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILAIMKE 374

Query: 327 TMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPLEFKPERFMG 386
           T+RLHP  P+LVP    E++ + GY I K T++FVNVW+I RDP VWENP EF+PERF+ 
Sbjct: 375 TLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPERFLD 434

Query: 387 KN-IDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMD 446
            N  D  G ++   PFGSGRR+C G  L  +++  +LA LLH F WK+    +   L++ 
Sbjct: 435 NNSCDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIP---EGHVLDLK 494

Query: 447 EVFGLSTPKKFPLDVVAEPRLSPS 467
           E FG+    K PL  +  PR S S
Sbjct: 495 EKFGIVLKLKIPLVALPIPRFSDS 512

BLAST of PI0013250 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 332.0 bits (850), Expect = 7.6e-91
Identity = 180/461 (39.05%), Postives = 277/461 (60.09%), Query Frame = 0

Query: 16  PIHSSTLQKIR----PIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTT 75
           PI    L K+     P+M+L  GS P ++ SS EMA   LK+ +LNF++RP      Y T
Sbjct: 52  PIAHQALHKLSIRYGPLMYLFIGSIPNLIVSSAEMANEILKSNELNFLNRPTMQNVDYLT 111

Query: 76  YNYSDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYK--SCGEVI 135
           Y  +D   + YG +W+  +++C++ELFS+R LDS+  +R EE+K LL  + K     E +
Sbjct: 112 YGSADFFSAPYGLHWKFMKRICMVELFSSRALDSFVSVRSEELKKLLIRVLKKAEAEESV 171

Query: 136 KLKDYLSTVSLNVISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWI 195
            L + L  ++ N+I+RM+  K  +D   +G    +E  KM+ EL  L+G  N+ ++  ++
Sbjct: 172 NLGEQLKELTSNIITRMMFRKMQSD--SDGGEKSEEVIKMVVELNELAGFFNVSETFWFL 231

Query: 196 DFLDLQGYVKRMKVLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEV 255
             LDLQG  KR+K    K+D  +E +++EH   +K       ++M+DVLL + +D + E+
Sbjct: 232 KRLDLQGLKKRLKNARDKYDVIIERIMEEHESSKKNATG--ERNMLDVLLDIYEDKNAEM 291

Query: 256 KLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEK 315
           KL R  +KAF  ++  GGT++SA+TVEWA++EL+  PEI  KA++E+++V+G +R VEE 
Sbjct: 292 KLTRENIKAFIMNIYGGGTDTSAITVEWALAELINHPEIMKKAQQEIEQVVGNKRVVEES 351

Query: 316 DIVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTV 375
           D+ NL Y  A+ KETMRLHP  P+ V R + E+  +AG+ I   TRV VNVW IGRD   
Sbjct: 352 DLCNLSYTQAVVKETMRLHPGGPIFV-RESDEECAVAGFRIPAKTRVIVNVWAIGRDSNQ 411

Query: 376 WENPLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWK 435
           WE+PLEF+PERF G    V  +  +++ FG+GRR CPG  +  + +   LA ++  F  K
Sbjct: 412 WEDPLEFRPERFEGSEWKVMSE--KMMSFGAGRRSCPGEKMVFRFVPIILAAIIQCFELK 471

Query: 436 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLYSI 471
           + G      ++MDE  G S P+  PL  V   + +   +S+
Sbjct: 472 VKG-----SVDMDEGTGSSLPRATPLVCVPVAKEATQSFSL 500

BLAST of PI0013250 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 327.4 bits (838), Expect = 1.9e-89
Identity = 168/454 (37.00%), Postives = 262/454 (57.71%), Query Frame = 0

Query: 23  QKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQY 82
           Q   PI+ +  GS   VV SS E+AK  LK  D     R +  + +  + N  D+ W+ Y
Sbjct: 56  QSYGPIISVWIGSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADY 115

Query: 83  GPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSC------GEVIKLKDYLS 142
           GP++ + RK+C +ELF+ +RL+S   IR++E+ A++  +++ C       + ++L+ YL 
Sbjct: 116 GPHYVKVRKVCTLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLG 175

Query: 143 TVSLNVISRMVLGKKYTDESENGIVSPD--EFKKMLDELFLLSGVLNIGDSIPWIDFLDL 202
            V+ N I+R+  GK++   +  G+V     EFK ++     L   L+I + IPW+ ++  
Sbjct: 176 AVAFNNITRLAFGKRFM--NAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWM-F 235

Query: 203 QGYVKRMKVLSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQLADDPDLEVKLERH 262
               K       + DR    +++EH   R+   +   +  VD LL L D  D    L   
Sbjct: 236 PADEKAFAEHGARRDRLTRAIMEEHTLARQ-KSSGAKQHFVDALLTLKDQYD----LSED 295

Query: 263 GVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIVNL 322
            +     D+I  G +++A+T EWAM+E++K P +  K +EE DRV+G +R + E D   L
Sbjct: 296 TIIGLLWDMITAGMDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRL 355

Query: 323 PYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTVWENPL 382
           PY+  + KE+ RLHP  P+++P  +  D +I GYDI KG+ V VNVW + RDP VW+NP 
Sbjct: 356 PYLQCVVKESFRLHPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPF 415

Query: 383 EFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWKLSGDM 442
           EF+PERF+ +++D+KG DF LLPFG+GRR+CPG  LG+ ++ S +++LLH F W      
Sbjct: 416 EFRPERFLEEDVDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGT 475

Query: 443 KNEDLNMDEVFGLSTPKKFPLDVVAEPRLSPSLY 469
           K E+++M E  GL T  + P+  VA PRL   LY
Sbjct: 476 KPEEIDMSENPGLVTYMRTPVQAVATPRLPSDLY 501

BLAST of PI0013250 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 323.6 bits (828), Expect = 2.7e-88
Identity = 183/455 (40.22%), Postives = 269/455 (59.12%), Query Frame = 0

Query: 20  STLQKIRPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITW 79
           S  +K  P+M L+FGS P VV SS E AK  LK  DLN  SRP  A  +  +YNY DI +
Sbjct: 57  SLSKKYGPVMLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVF 116

Query: 80  SQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMKALLREIYKSCGE--VIKLKDYLST 139
           S +  YW++ R+MC+ ELFS +++   + IR+EE+K L+    +S  +   + L + L++
Sbjct: 117 SPFNDYWKELRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLAS 176

Query: 140 VSLNVISRMVLGKKYTDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIP---W-IDFL- 199
           +++ VI +   G  +    +  +++ D F K++ + FL  G  +  D  P   W ID+L 
Sbjct: 177 LTVGVICKAAFGVSF----QGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLT 236

Query: 200 DLQGYVKRMKVLSKKFDRFLEHVLDEHNE-RRKGVENYVAKDMVDVLLQLADDPDL--EV 259
            LQG  +R     +  D F E + D H +  ++GVE     D VD+LL+L  +  +    
Sbjct: 237 GLQGQRERS---VRGLDAFYEQMFDLHKQGNKEGVE-----DFVDLLLKLEKEETVLGYG 296

Query: 260 KLERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEK 319
           KL R+ +KA   +++ GG  +SA+T+ WAM+EL++ P +  K + E+   IG +  +   
Sbjct: 297 KLTRNHIKAVLMNVLLGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLD 356

Query: 320 DIVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVFVNVWTIGRDPTV 379
           DI  L Y+  +  ET RLHP AP+LVPR    + +I GY I   TR++VNVW IGRDP  
Sbjct: 357 DIDQLHYLKMVINETWRLHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDT 416

Query: 380 WENPLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHGFTWK 439
           W++P EF PERF+  NID KGQ+FELLPFGSGRRMCP   +G  +++  LANLL+ F WK
Sbjct: 417 WKDPEEFLPERFVNSNIDAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWK 476

Query: 440 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRLS 465
           L   M  ED++M+E  GL+  KK  L +V    L+
Sbjct: 477 LPEGMVVEDIDMEESPGLNASKKNELVLVPRKYLN 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1D6F9Y92.2e-12749.57Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1[more]
A0A1D6HSP42.6e-12047.82Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1[more]
P244651.1e-11045.13Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Q9SBQ95.3e-10542.08Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
A0A4D6Q4154.5e-10441.20Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Match NameE-valueIdentityDescription
A0A5D3D8H93.0e-25296.88Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CCA66.7e-25296.65cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103499163 PE=3 SV=1[more]
A0A6J1GXG66.9e-24191.74cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=... [more]
A0A6J1IRR81.9e-23890.63cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1[more]
A0A5D3C5I83.6e-23791.09Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
KAA0053469.16.2e-25296.88cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYK19885.1 cytochrome P450... [more]
XP_008460308.11.4e-25196.65PREDICTED: cytochrome P450 71A1-like [Cucumis melo][more]
XP_004144428.37.1e-24895.76trimethyltridecatetraene synthase [Cucumis sativus] >KGN58597.2 hypothetical pro... [more]
XP_038876425.11.2e-24489.73trimethyltridecatetraene synthase-like [Benincasa hispida][more]
XP_022956310.11.4e-24091.74cytochrome P450 71A1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G07990.13.9e-10341.91Cytochrome P450 superfamily protein [more]
AT4G12300.15.8e-9139.86cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT5G06900.17.6e-9139.05cytochrome P450, family 93, subfamily D, polypeptide 1 [more]
AT2G40890.11.9e-8937.00cytochrome P450, family 98, subfamily A, polypeptide 3 [more]
AT3G26330.12.7e-8840.22cytochrome P450, family 71, subfamily B, polypeptide 37 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 138..156
score: 23.95
coord: 22..41
score: 30.27
coord: 46..67
score: 24.64
coord: 275..301
score: 40.02
coord: 318..336
score: 36.47
coord: 395..405
score: 55.71
coord: 255..272
score: 24.33
coord: 359..383
score: 39.32
coord: 405..428
score: 27.82
IPR001128Cytochrome P450PRINTSPR00385P450coord: 319..330
score: 39.41
coord: 266..283
score: 27.97
coord: 396..405
score: 59.44
IPR001128Cytochrome P450PFAMPF00067p450coord: 23..449
e-value: 2.2E-89
score: 300.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 14..462
e-value: 2.0E-113
score: 381.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 20..462
NoneNo IPR availablePANTHERPTHR24281STEROID 21-HYDROXYLASE-RELATEDcoord: 17..461
NoneNo IPR availablePANTHERPTHR24281:SF126CYTOCHROME P450 71A1-LIKEcoord: 17..461
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 398..407
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 154..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013250.1PI0013250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen