PI0013216 (gene) Melon (PI 482460) v1

BLAST of PI0013216 vs. ExPASy Swiss-Prot
Match: Q6BE24 (Cucurbitadienol synthase OS=Cucurbita pepo OX=3663 GN=CPQ PE=1 SV=1)

HSP 1 Score: 1299.3 bits (3361), Expect = 0.0e+00
Identity = 620/784 (79.08%), Postives = 662/784 (84.44%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGE++EKW+KS+SNHLGRQVWEFC +                 A++   L
Sbjct: 1   MWRLKVGAESVGEEDEKWVKSVSNHLGRQVWEFCAD---------------AAADTPHQL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFL---------------------- 120
           L     Q +NAR HF +NRFHRKQSSDLFLAIQ    +                      
Sbjct: 61  L-----QIQNARNHFHHNRFHRKQSSDLFLAIQYEKEIAKGAKGGAVKVKEGEEVGKEAV 120

Query: 121 -STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            ST      +Y +    DGNWASDLGGP+FLLPGLVIAL+VTGVLNSVLSKHHR EMCRY
Sbjct: 121 KSTLERALGFYSAVQTRDGNWASDLGGPLFLLPGLVIALHVTGVLNSVLSKHHRVEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGG+GGAMTKAR+WIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGDGGAMTKARAWILERGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITP VLSLR+ELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQD+LWGSIYH+YEPL
Sbjct: 301 FVGPITPKVLSLRQELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA++ AM+HIHYEDEN           +LNMLCCWVEDPYSDAFK HLQ
Sbjct: 361 FTRWPGKRLREKALQAAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RV DYLW+AEDGMRMQGYNGSQLWDTAFSIQAI++TKL+D++  TL+KAH FVK SQIQE
Sbjct: 421 RVHDYLWVAEDGMRMQGYNGSQLWDTAFSIQAIVATKLVDSYAPTLRKAHDFVKDSQIQE 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGDPNVWFRHIHKGAWP STRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRL
Sbjct: 481 DCPGDPNVWFRHIHKGAWPLSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQN+NGGFASYELTRSYPWLELINPAETFGDIVIDY YVECT+ATMEAL L
Sbjct: 541 CDAVNVLLSLQNDNGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID A+ KAANFLE MQR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDTAIGKAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           N+C+AIRKAC FLLSKELPGGGWG        +VYTNLEGNKPHLVNTAWV+MALIEAGQ
Sbjct: 661 NSCLAIRKACEFLLSKELPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
           GERDPAPLHRAARLL+NSQLENGDF QQEIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 GERDPAPLHRAARLLMNSQLENGDFVQQEIMGVFNKNCMITYAAYRNIFPIWALGEYCHR 764

BLAST of PI0013216 vs. ExPASy Swiss-Prot
Match: K7NBZ9 (Cucurbitadienol synthase OS=Siraitia grosvenorii OX=190515 GN=CBQ PE=1 SV=1)

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 607/784 (77.42%), Postives = 651/784 (83.04%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGE +EKW+KSISNHLGRQVWEFC +                 A + + L
Sbjct: 1   MWRLKVGAESVGENDEKWLKSISNHLGRQVWEFCPD-----------------AGTQQQL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQV-----------------------RSF 120
           LQ  +     ARK F ++RFHRKQSSDLF+ IQ                         + 
Sbjct: 61  LQVHK-----ARKAFHDDRFHRKQSSDLFITIQYGKEVENGGKTAGVKLKEGEEVRKEAV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            S+      +Y S    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY
Sbjct: 121 ESSLERALSFYSSIQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG STMFGSALNYVALRLLGEDA+    GAM KAR+WIL  GGAT
Sbjct: 181 VYNHQNEDGGWGLHIEGPSTMFGSALNYVALRLLGEDAN---AGAMPKARAWILDHGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
            ITSWGK            GNNPLPPEFWL PY LPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 GITSWGKLWLSVLGVYEWSGNNPLPPEFWLFPYFLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITPIVLSLRKELY +PYHEIDWNKSRNTCAKEDLYYPHPKMQD+LWGS++H+YEPL
Sbjct: 301 FVGPITPIVLSLRKELYAVPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSLHHVYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WP KRLREKA++ AM+HIHYEDEN           +LN+LCCWVEDPYSDAFK HLQ
Sbjct: 361 FTRWPAKRLREKALQTAMQHIHYEDENTRYICLGPVNKVLNLLCCWVEDPYSDAFKLHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RV DYLW+AEDGM+MQGYNGSQLWDTAFSIQAI+STKL+D +G TL+KAH FVK SQIQ+
Sbjct: 421 RVHDYLWVAEDGMKMQGYNGSQLWDTAFSIQAIVSTKLVDNYGPTLRKAHDFVKSSQIQQ 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGDPNVW+RHIHKGAWPFSTRDHGWLISDCTAEGLKA+LMLSKLPS+ VGE LE+NRL
Sbjct: 481 DCPGDPNVWYRHIHKGAWPFSTRDHGWLISDCTAEGLKAALMLSKLPSETVGESLERNRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQN+NGGFASYELTRSYPWLELINPAETFGDIVIDY YVECTSATMEAL L
Sbjct: 541 CDAVNVLLSLQNDNGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID A+ +AANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDTAIVRAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           NNC+AIRKAC FLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MALIEAGQ
Sbjct: 661 NNCLAIRKACDFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            ERDP PLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 AERDPTPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYCHR 759

BLAST of PI0013216 vs. ExPASy Swiss-Prot
Match: Q9SLP9 (Cycloartenol synthase OS=Luffa aegyptiaca OX=3670 GN=CAS1 PE=1 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 3.2e-306
Identity = 490/785 (62.42%), Postives = 599/785 (76.31%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEK---WIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSS 60
           MW+LK+G ++V         W+ S++NH+GRQVW F  E     +               
Sbjct: 1   MWQLKIGADTVPADPSNAGGWLSSLNNHVGRQVWHFHPELGTPED--------------- 60

Query: 61  KHLLQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFLSTF---PFFYL-------- 120
                   +Q ++AR+ F ++RF +K S+DL + +Q     S+F   P   +        
Sbjct: 61  -------LQQIQHARQRFSDHRFEKKHSADLLMRMQFAKNNSSFVNLPQIKVKDKEDVTE 120

Query: 121 ------------YYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEM 180
                       +Y +    DG+W  D GGPMFL+PGLVI L +TG LN+VLS  H++E+
Sbjct: 121 EAVSRTLRRAINFYSTIQGDDGHWPGDYGGPMFLIPGLVITLSITGALNAVLSTEHQREI 180

Query: 181 CRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRG 240
           CRY+YNHQN+DGGWGLHIEG STMFGS LNYV+LRLLGE+A+ G+ GA+ KAR WIL  G
Sbjct: 181 CRYLYNHQNKDGGWGLHIEGPSTMFGSVLNYVSLRLLGEEAEDGQ-GAVDKARKWILDHG 240

Query: 241 GATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLY 300
           GA+AITSWGK            GNNPLPPE WLLPY LPFHPGRMWCHCRMVYLPM YLY
Sbjct: 241 GASAITSWGKMWLSVLGVYEWAGNNPLPPELWLLPYLLPFHPGRMWCHCRMVYLPMCYLY 300

Query: 301 GKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLY 360
           GKRFVGPITPI+ SLRKELY +PYHE+DWNK+RN CAKEDLYYPHP +QD++W S++H+Y
Sbjct: 301 GKRFVGPITPIIRSLRKELYLVPYHEVDWNKARNECAKEDLYYPHPLVQDIVWASLHHVY 360

Query: 361 EPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKF 420
           EPLF  WP KRLREKA++  M+HIHYEDEN           +LNMLCCWVEDP+S+AFK 
Sbjct: 361 EPLFMRWPAKRLREKALQCVMQHIHYEDENTRYICIGPVNKVLNMLCCWVEDPHSEAFKL 420

Query: 421 HLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQ 480
           H+ R+ DYLW+AEDGM+MQGYNGSQLWDTAF++QAI+STKL + +G TL+KAH ++K SQ
Sbjct: 421 HIPRIFDYLWIAEDGMKMQGYNGSQLWDTAFAVQAIMSTKLAEEYGTTLRKAHKYIKDSQ 480

Query: 481 IQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK 540
           + EDCPGD   W+RHI KGAWPFST DHGW ISDCTAEGLKA L+LSKLPS+IVG+ +++
Sbjct: 481 VLEDCPGDLQSWYRHISKGAWPFSTADHGWPISDCTAEGLKAVLLLSKLPSEIVGKSIDE 540

Query: 541 NRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEA 600
            ++ DAVNV+LSLQN +GGFA+YELTRSYPWLEL+NPAETFGDIVIDY+YVECTSA ++A
Sbjct: 541 EQIYDAVNVILSLQNTDGGFATYELTRSYPWLELMNPAETFGDIVIDYTYVECTSAAIQA 600

Query: 601 LMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAG 660
           L+ FKKL+PGHR  EID  VAKAA+F+E++Q TDGSWYG WGVCFTY GWFGI+GLVAAG
Sbjct: 601 LVAFKKLYPGHRRDEIDNCVAKAADFIESIQATDGSWYGSWGVCFTYGGWFGIRGLVAAG 660

Query: 661 RTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIE 720
           R Y+NC ++RKAC FLLSKEL  GGWG        +VYTN++ ++PH+VNT W M++LI+
Sbjct: 661 RRYDNCSSLRKACDFLLSKELASGGWGESYLSGQNKVYTNIKDDRPHIVNTGWAMLSLID 720

Query: 721 AGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEY 730
           AGQ ERDP PLHRAAR+LINSQ+++GDFPQ+EIMG+FNKNCMI+YAAYRNIFPIWALGEY
Sbjct: 721 AGQSERDPTPLHRAARILINSQMDDGDFPQEEIMGIFNKNCMISYAAYRNIFPIWALGEY 762

BLAST of PI0013216 vs. ExPASy Swiss-Prot
Match: Q8W3Z4 (Cycloartenol synthase OS=Betula platyphylla OX=78630 GN=CASBPX1 PE=1 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 1.2e-305
Identity = 498/788 (63.20%), Postives = 582/788 (73.86%), Query Frame = 0

Query: 1   MWRLKVGKESV------GEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVA 60
           MW+LK+G E+       G   E W++S++NHLGRQ+WEF  E   + E +  DD      
Sbjct: 1   MWKLKIGAETARGDGGGGGGSETWLRSLNNHLGRQIWEFHPELGTQEELQQIDD------ 60

Query: 61  NSSKHLLQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQV-------------------- 120
                           AR+ F   RF R+ SSDL + IQ                     
Sbjct: 61  ----------------ARRRFWERRFERRHSSDLLMRIQFAKENPSSANIPQVKIKDTEE 120

Query: 121 ---RSFLSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180
               +   T      +Y +    DG+W  D GGPMFL+PGLVI L +TG LN+ LSK H+
Sbjct: 121 VREEAVGMTLRRAINFYSTIQADDGHWPGDYGGPMFLIPGLVITLSITGTLNAFLSKEHQ 180

Query: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL 240
            E+CRY+YNHQNEDGGWGLHIEG STMFG+ALNY+ LRLLGE  DG   GA+ KAR WIL
Sbjct: 181 CEICRYLYNHQNEDGGWGLHIEGPSTMFGTALNYITLRLLGEPEDG--MGAVEKARKWIL 240

Query: 241 GRGGATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMS 300
             GGATAITSWGK            GNNPLPPE WL PY LP HPGRMWCHCRMVYLPMS
Sbjct: 241 DHGGATAITSWGKMWLSVLGVYEWSGNNPLPPEVWLCPYLLPCHPGRMWCHCRMVYLPMS 300

Query: 301 YLYGKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIY 360
           YLYGKRFVGPIT  + SLRKELYT+PYHEIDWNK+RN CAKEDLYYPHP +QD+LW S+Y
Sbjct: 301 YLYGKRFVGPITSTIQSLRKELYTVPYHEIDWNKARNDCAKEDLYYPHPLVQDILWASLY 360

Query: 361 HLYEPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDA 420
           + YEP+F  WP KRLREKA+   M+HIHYEDEN           +LNMLCCW EDP S+A
Sbjct: 361 YAYEPIFMYWPAKRLREKALDTVMQHIHYEDENTRYICIGPVNKVLNMLCCWAEDPNSEA 420

Query: 421 FKFHLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVK 480
           FK HL R+ DYLW+AEDGM+MQGYNGSQLWDT F++QAI+ST + + +GQTL+KAH ++K
Sbjct: 421 FKLHLPRILDYLWIAEDGMKMQGYNGSQLWDTTFAVQAIISTNIAEEYGQTLRKAHEYIK 480

Query: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540
            SQ+ EDCPGD N W+RHI KGAWPFST DHGW ISDCTAEGLKA ++LS+ PS+ VG+ 
Sbjct: 481 DSQVLEDCPGDLNFWYRHISKGAWPFSTADHGWPISDCTAEGLKAVILLSQFPSETVGKS 540

Query: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600
           ++  RL DAV+V+LSLQN +GGFA+YELTRSY WLELINPAETFGDIVIDY YVECTSA 
Sbjct: 541 VDVKRLYDAVHVILSLQNTDGGFATYELTRSYHWLELINPAETFGDIVIDYPYVECTSAA 600

Query: 601 MEALMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660
           ++AL LFKKLHPGHR +EI+  +AKAA F+EN+Q +DGSWYG WGVCFTYAGWFGIKGLV
Sbjct: 601 IQALTLFKKLHPGHRREEIENCIAKAAEFIENIQASDGSWYGSWGVCFTYAGWFGIKGLV 660

Query: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMA 720
           AAGRTY NC +I KAC +LLSKEL  GGWG        +VYTNL+ N+PH+VNT W M+A
Sbjct: 661 AAGRTYKNCSSIHKACDYLLSKELASGGWGESYLSCQDKVYTNLKDNRPHIVNTGWAMLA 720

Query: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 730
           LI+AGQ ERDP PLHRAAR+LINSQ+ENGDFPQ+EIMGVFNKNCMI+Y+AYRNIFPIWAL
Sbjct: 721 LIDAGQAERDPTPLHRAARILINSQMENGDFPQEEIMGVFNKNCMISYSAYRNIFPIWAL 764

BLAST of PI0013216 vs. ExPASy Swiss-Prot
Match: Q6BE25 (Cycloartenol synthase OS=Cucurbita pepo OX=3663 GN=CPX PE=1 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 4.7e-302
Identity = 483/785 (61.53%), Postives = 590/785 (75.16%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEK---WIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSS 60
           MW+LK+G ++V         W+ +++NH+GRQVW F  E  +  +               
Sbjct: 1   MWQLKIGADTVPSDPSNAGGWLSTLNNHVGRQVWHFHPELGSPED--------------- 60

Query: 61  KHLLQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFLSTFPFF------------- 120
                   +Q + AR+HF ++RF +K S+DL + +Q     S+F                
Sbjct: 61  -------LQQIQQARQHFSDHRFEKKHSADLLMRMQFAKENSSFVNLPQVKVKDKEDVTE 120

Query: 121 ----------YLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEM 180
                       +Y +    DG+W  D GGPMFL+PGLVI L +TG LN+VLS  H++E+
Sbjct: 121 EAVTRTLRRAINFYSTIQADDGHWPGDYGGPMFLIPGLVITLSITGALNAVLSTEHQREI 180

Query: 181 CRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRG 240
           CRY+YNHQN+DGGWGLHIEG STMFGS LNYV LRLLGE+A+ G+ GA+ KAR WIL  G
Sbjct: 181 CRYLYNHQNKDGGWGLHIEGPSTMFGSVLNYVTLRLLGEEAEDGQ-GAVDKARKWILDHG 240

Query: 241 GATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLY 300
           GA AITSWGK            GNNPLPPE WLLPY LP HPGRMWCHCRMVYLPM YLY
Sbjct: 241 GAAAITSWGKMWLSVLGVYEWAGNNPLPPELWLLPYLLPCHPGRMWCHCRMVYLPMCYLY 300

Query: 301 GKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLY 360
           GKRFVGPITPI+ SLRKELY +PYHE+DWNK+RN CAKEDLYYPHP +QD+LW +++H+Y
Sbjct: 301 GKRFVGPITPIIRSLRKELYLVPYHEVDWNKARNQCAKEDLYYPHPLVQDILWATLHHVY 360

Query: 361 EPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKF 420
           EPLF  WP KRLREKA++  M+HIHYEDEN           +LNMLCCW EDP+S+AFK 
Sbjct: 361 EPLFMHWPAKRLREKALQSVMQHIHYEDENTRYICIGPVNKVLNMLCCWAEDPHSEAFKL 420

Query: 421 HLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQ 480
           H+ R+ DYLW+AEDGM+MQGYNGSQLWDTAF++QAI+ST+L + +  TL+KAH ++K SQ
Sbjct: 421 HIPRIYDYLWIAEDGMKMQGYNGSQLWDTAFAVQAIISTELAEEYETTLRKAHKYIKDSQ 480

Query: 481 IQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK 540
           + EDCPGD   W+RHI KGAWPFST DHGW ISDCTAEGLKA L+LSKLPS+IVG+ +++
Sbjct: 481 VLEDCPGDLQSWYRHISKGAWPFSTADHGWPISDCTAEGLKAVLLLSKLPSEIVGKSIDE 540

Query: 541 NRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEA 600
            +L +AVNV+LSLQN +GGFA+YELTRSY WLEL+NPAETFGDIVIDY YVEC+SA ++A
Sbjct: 541 QQLYNAVNVILSLQNTDGGFATYELTRSYRWLELMNPAETFGDIVIDYPYVECSSAAIQA 600

Query: 601 LMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAG 660
           L  FKKL+PGHR  EID  +A+AA+F+E++Q TDGSWYG WGVCFTY GWFGI+GLVAAG
Sbjct: 601 LAAFKKLYPGHRRDEIDNCIAEAADFIESIQATDGSWYGSWGVCFTYGGWFGIRGLVAAG 660

Query: 661 RTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIE 720
           R YNNC ++RKAC FLLSKEL  GGWG        +VYTN++ ++PH+VNT W M++LI+
Sbjct: 661 RRYNNCSSLRKACDFLLSKELAAGGWGESYLSCQNKVYTNIKDDRPHIVNTGWAMLSLID 720

Query: 721 AGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEY 730
           AGQ ERDP PLHRAAR+LINSQ+E+GDFPQ+EIMGVFNKNCMI+Y+AYRNIFPIWALGEY
Sbjct: 721 AGQSERDPTPLHRAARVLINSQMEDGDFPQEEIMGVFNKNCMISYSAYRNIFPIWALGEY 762

BLAST of PI0013216 vs. ExPASy TrEMBL
Match: A0A097IYL3 (Terpene cyclase/mutase family member OS=Cucumis sativus OX=3659 GN=Csa6G088690 PE=2 SV=1)

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 677/790 (85.70%), Postives = 700/790 (88.61%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAI--VVANSSK 60
           MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC EN+     ++DDDEA+  VVANSSK
Sbjct: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCAEND-----DDDDDEAVIHVVANSSK 60

Query: 61  HLLQPQRRQ--FENARKHFRNNRFHRKQSSDLFLAIQVRSFL------------------ 120
           HLLQ QRRQ  FENARK FRNNRFHRKQSSDLFL IQ    +                  
Sbjct: 61  HLLQQQRRQSSFENARKQFRNNRFHRKQSSDLFLTIQYEKEIARNGAKNGGNTKVKEGED 120

Query: 121 -------STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180
                  +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR
Sbjct: 121 VKKEAVNNTLERALSFYSAIQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180

Query: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL 240
           QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDA+GGE GAMTKARSWIL
Sbjct: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDANGGECGAMTKARSWIL 240

Query: 241 GRGGATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMS 300
            RGGATAITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMS
Sbjct: 241 ERGGATAITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMS 300

Query: 301 YLYGKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIY 360
           YLYGKRFVGPIT +VLSLRKELYTIPYHEIDWN+SRNTCA+EDLYYPHPKMQD+LWGSIY
Sbjct: 301 YLYGKRFVGPITHMVLSLRKELYTIPYHEIDWNRSRNTCAQEDLYYPHPKMQDILWGSIY 360

Query: 361 HLYEPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDA 420
           H+YEPLF+GWPG+RLREKAMKIAMEHIHYEDEN           +LNMLCCWVEDPYSDA
Sbjct: 361 HVYEPLFNGWPGRRLREKAMKIAMEHIHYEDENSRYIYLGPVNKVLNMLCCWVEDPYSDA 420

Query: 421 FKFHLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVK 480
           FKFHLQR+PDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFG TL+KAHHFVK
Sbjct: 421 FKFHLQRIPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGSTLRKAHHFVK 480

Query: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540
           HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP
Sbjct: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540

Query: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600
           LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT
Sbjct: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600

Query: 601 MEALMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660
           MEAL LFKKLHPGHRTKEIDAA+AKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV
Sbjct: 601 MEALALFKKLHPGHRTKEIDAALAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660

Query: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMA 720
           AAGRTYNNCVAIRKACHFLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MA
Sbjct: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMA 720

Query: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 732
           LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL
Sbjct: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 780

BLAST of PI0013216 vs. ExPASy TrEMBL
Match: A0A1S3CBF6 (Terpene cyclase/mutase family member OS=Cucumis melo OX=3656 GN=LOC103498907 PE=3 SV=1)

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 671/784 (85.59%), Postives = 689/784 (87.88%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC       ENENDDDEAI VAN+S   
Sbjct: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC-----SGENENDDDEAIAVANNS--- 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFL---------------------- 120
                 +FENAR HFRNNRFHRKQSSDLFLAIQ    +                      
Sbjct: 61  ----ASKFENARNHFRNNRFHRKQSSDLFLAIQCEKEIIRNGAKNEGTTKVKEGEDVKKE 120

Query: 121 ---STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC 180
              +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC
Sbjct: 121 AVKNTLERALSFYSAVQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC 180

Query: 181 RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGG 240
           RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGE ADGGE GAMTKARSWIL RGG
Sbjct: 181 RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEAADGGEHGAMTKARSWILERGG 240

Query: 241 ATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYG 300
           ATAITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYG
Sbjct: 241 ATAITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYG 300

Query: 301 KRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYE 360
           KRFVGPITPIVLSLRKELYTIPYHEIDWN+SRNTCAKEDLYYPHPKMQD+LWGSIYH+YE
Sbjct: 301 KRFVGPITPIVLSLRKELYTIPYHEIDWNRSRNTCAKEDLYYPHPKMQDILWGSIYHVYE 360

Query: 361 PLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFH 420
           PLFSGWPGKRLREKAMKIAMEHIHYEDEN           +LNMLCCWVEDPYSDAFKFH
Sbjct: 361 PLFSGWPGKRLREKAMKIAMEHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFH 420

Query: 421 LQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQI 480
           LQR+PDYLWLAEDGMRMQGYNGSQLWDTAFSIQAI+STKLIDTFG TL+KAHHFVKHSQI
Sbjct: 421 LQRIPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAIISTKLIDTFGPTLRKAHHFVKHSQI 480

Query: 481 QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN 540
           QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN
Sbjct: 481 QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN 540

Query: 541 RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL 600
           RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL
Sbjct: 541 RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL 600

Query: 601 MLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGR 660
            LFKKLHPGHRTKEIDAA+AKAANFLENMQ+TDGSWYGCWGVCFTYAGWFGIKGLVAAGR
Sbjct: 601 ALFKKLHPGHRTKEIDAAIAKAANFLENMQKTDGSWYGCWGVCFTYAGWFGIKGLVAAGR 660

Query: 661 TYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEA 720
           TYNNCVAIRKAC+FLLSKELPGGGWG        +VYTNLEGNKPHLVNTAWVMMALIEA
Sbjct: 661 TYNNCVAIRKACNFLLSKELPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVMMALIEA 720

Query: 721 GQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS 730
           GQGERDPAPLHRAARLLINSQLE+GDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS
Sbjct: 721 GQGERDPAPLHRAARLLINSQLESGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS 772

BLAST of PI0013216 vs. ExPASy TrEMBL
Match: A0A0D3QY32 (Terpene cyclase/mutase family member OS=Citrullus colocynthis OX=252529 GN=CDS1 PE=2 SV=1)

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 644/784 (82.14%), Postives = 674/784 (85.97%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGEKEEKW+KSISNHLGRQVWEFC      + N               HL
Sbjct: 1   MWRLKVGAESVGEKEEKWLKSISNHLGRQVWEFCAHQPTASPN---------------HL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ-----------------------VRSF 120
                +Q +NAR HFRNNRFHRKQSSDLFLAIQ                         + 
Sbjct: 61  -----QQIDNARNHFRNNRFHRKQSSDLFLAIQNEKEIANVTKGGGIKVKEEEDVRKETV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY
Sbjct: 121 KNTVERALSFYSAIQTNDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILDRGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITPIVLSLRKELYTIPYHEIDWN+SRNTCAKEDLYYPHPKMQD+LWGSIYHLYEPL
Sbjct: 301 FVGPITPIVLSLRKELYTIPYHEIDWNRSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA+++AM+HIHYEDEN           +LNMLCCWVEDPYSDAFKFHLQ
Sbjct: 361 FTRWPGKRLREKALQMAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RVPDYLW+AEDGMRMQGYNGSQLWDTAFS+QAI+STKLID+FG TLKKAH FVK SQIQ+
Sbjct: 421 RVPDYLWVAEDGMRMQGYNGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQ 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK+RL
Sbjct: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDY YVECTSATMEAL L
Sbjct: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID AVA+AANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDIAVARAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           N+CVAIRKAC FLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MALIEAGQ
Sbjct: 661 NSCVAIRKACDFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            ERDPAPLHRAARLLINSQLENGDFPQ+EIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 AERDPAPLHRAARLLINSQLENGDFPQEEIMGVFNKNCMITYAAYRNIFPIWALGEYFHR 764

BLAST of PI0013216 vs. ExPASy TrEMBL
Match: A0A0D3QXV2 (Terpene cyclase/mutase family member OS=Citrullus colocynthis OX=252529 GN=CDS2 PE=2 SV=1)

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 644/784 (82.14%), Postives = 673/784 (85.84%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGEKEEKW+KSISNHLGRQVWEFC +    + N               HL
Sbjct: 1   MWRLKVGAESVGEKEEKWLKSISNHLGRQVWEFCADQPTASPN---------------HL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ-----------------------VRSF 120
                +Q +NARKHFRNNRFHRKQSSDLFLAIQ                         + 
Sbjct: 61  -----QQIDNARKHFRNNRFHRKQSSDLFLAIQNEKEIANGTKGGGIKVKEEEDVRKETV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY
Sbjct: 121 KNTVERALSFYSAIQTNDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGGEGGAMTKAR WIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGEGGAMTKARGWILDRGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQD+LWGSIYHLYEPL
Sbjct: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA+++AM+HIHYEDEN           +LNMLCCWVEDPYSDAFKFHLQ
Sbjct: 361 FTRWPGKRLREKALQMAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RVPDYLW+AEDGMRMQGYNGSQLWDTAFS+QAI+STKLID+FG TLKKAH FVK SQIQ+
Sbjct: 421 RVPDYLWIAEDGMRMQGYNGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQ 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           D PGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK+RL
Sbjct: 481 DFPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDY YVECTSATMEAL L
Sbjct: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID AVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDTAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           + CVAIRKAC FLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MALIEAGQ
Sbjct: 661 STCVAIRKACDFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            ERDPAPLHRAARLLINSQLENGDFPQ+EIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 AERDPAPLHRAARLLINSQLENGDFPQEEIMGVFNKNCMITYAAYRNIFPIWALGEYFHR 764

BLAST of PI0013216 vs. ExPASy TrEMBL
Match: A0A6J1GP10 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111455830 PE=3 SV=1)

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 624/784 (79.59%), Postives = 661/784 (84.31%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGEK+EKW+KS+SNHLGRQVWEFC +                 A + + L
Sbjct: 1   MWRLKVGAESVGEKDEKWVKSVSNHLGRQVWEFCAD--------------AAAAATPRQL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFL---------------------- 120
           L     Q +NAR HF  NRFHRKQSSDLFLAIQ    +                      
Sbjct: 61  L-----QIQNARNHFHRNRFHRKQSSDLFLAIQYEKEIAEGGKGGAVKVKEEEEVGKEAV 120

Query: 121 -STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            ST      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR EMCRY
Sbjct: 121 KSTLERALSFYSAVQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRVEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGG+ GAMTKAR+WIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGDDGAMTKARAWILERGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITP VLSLR+ELYT+PYHEIDWNKSRNTCAKEDLYYPHPKMQD+LWGSIYH+YEPL
Sbjct: 301 FVGPITPKVLSLRQELYTVPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHVYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA++ AM+HIHYEDEN           +LNMLCCWVEDPYSDAFK HLQ
Sbjct: 361 FTRWPGKRLREKALQTAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKLHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RV DYLW+AEDGMRMQGYNGSQLWDTAFSIQAI++TKL+D+F  TL+KAH FVK SQIQE
Sbjct: 421 RVHDYLWVAEDGMRMQGYNGSQLWDTAFSIQAIVATKLVDSFAPTLRKAHDFVKDSQIQE 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPS +VGEPLEKNRL
Sbjct: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSTMVGEPLEKNRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQN+NGGFASYELTRSYPWLELINPAETFGDIVIDY YVECT+ATMEAL L
Sbjct: 541 CDAVNVLLSLQNDNGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTAATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID AV KAANFLE MQR DGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDTAVGKAANFLEKMQRADGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           N+C+AIRKAC FLLSKELPGGGWG        +VYTNLEGNKPHLVNTAWV+MALIEAGQ
Sbjct: 661 NSCLAIRKACEFLLSKELPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
           GERDPAPLHRAARLL+NSQLENGDF QQEIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 GERDPAPLHRAARLLMNSQLENGDFVQQEIMGVFNKNCMITYAAYRNIFPIWALGEYCHR 765

BLAST of PI0013216 vs. NCBI nr
Match: NP_001292630.1 (cucurbitadienol synthase [Cucumis sativus] >AIT72030.1 oxidosqualene cyclase [Cucumis sativus])

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 677/790 (85.70%), Postives = 700/790 (88.61%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAI--VVANSSK 60
           MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC EN+     ++DDDEA+  VVANSSK
Sbjct: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCAEND-----DDDDDEAVIHVVANSSK 60

Query: 61  HLLQPQRRQ--FENARKHFRNNRFHRKQSSDLFLAIQVRSFL------------------ 120
           HLLQ QRRQ  FENARK FRNNRFHRKQSSDLFL IQ    +                  
Sbjct: 61  HLLQQQRRQSSFENARKQFRNNRFHRKQSSDLFLTIQYEKEIARNGAKNGGNTKVKEGED 120

Query: 121 -------STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180
                  +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR
Sbjct: 121 VKKEAVNNTLERALSFYSAIQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180

Query: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL 240
           QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDA+GGE GAMTKARSWIL
Sbjct: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDANGGECGAMTKARSWIL 240

Query: 241 GRGGATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMS 300
            RGGATAITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMS
Sbjct: 241 ERGGATAITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMS 300

Query: 301 YLYGKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIY 360
           YLYGKRFVGPIT +VLSLRKELYTIPYHEIDWN+SRNTCA+EDLYYPHPKMQD+LWGSIY
Sbjct: 301 YLYGKRFVGPITHMVLSLRKELYTIPYHEIDWNRSRNTCAQEDLYYPHPKMQDILWGSIY 360

Query: 361 HLYEPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDA 420
           H+YEPLF+GWPG+RLREKAMKIAMEHIHYEDEN           +LNMLCCWVEDPYSDA
Sbjct: 361 HVYEPLFNGWPGRRLREKAMKIAMEHIHYEDENSRYIYLGPVNKVLNMLCCWVEDPYSDA 420

Query: 421 FKFHLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVK 480
           FKFHLQR+PDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFG TL+KAHHFVK
Sbjct: 421 FKFHLQRIPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGSTLRKAHHFVK 480

Query: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540
           HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP
Sbjct: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540

Query: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600
           LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT
Sbjct: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600

Query: 601 MEALMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660
           MEAL LFKKLHPGHRTKEIDAA+AKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV
Sbjct: 601 MEALALFKKLHPGHRTKEIDAALAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660

Query: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMA 720
           AAGRTYNNCVAIRKACHFLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MA
Sbjct: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMA 720

Query: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 732
           LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL
Sbjct: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 780

BLAST of PI0013216 vs. NCBI nr
Match: KGN46400.2 (hypothetical protein Csa_005166 [Cucumis sativus])

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 677/790 (85.70%), Postives = 700/790 (88.61%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAI--VVANSSK 60
           MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC EN+     ++DDDEA+  VVANSSK
Sbjct: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCAEND-----DDDDDEAVIHVVANSSK 60

Query: 61  HLLQPQRRQ--FENARKHFRNNRFHRKQSSDLFLAIQVRSFL------------------ 120
           HLLQ QRRQ  FENARK FRNNRFHRKQSSDLFL IQ    +                  
Sbjct: 61  HLLQQQRRQSSFENARKQFRNNRFHRKQSSDLFLTIQYEKEIARNGAKNGGNTKVKEGED 120

Query: 121 -------STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180
                  +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR
Sbjct: 121 VKKEAVNNTLERALSFYSAIQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180

Query: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL 240
           QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDA+GGE GAMTKARSWIL
Sbjct: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDANGGECGAMTKARSWIL 240

Query: 241 GRGGATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMS 300
            RGGATAITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMS
Sbjct: 241 ERGGATAITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMS 300

Query: 301 YLYGKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIY 360
           YLYGKRFVGPIT +VLSLRKELYTIPYHEIDWN+SRNTCA+EDLYYPHPKMQD+LWGSIY
Sbjct: 301 YLYGKRFVGPITHMVLSLRKELYTIPYHEIDWNRSRNTCAQEDLYYPHPKMQDILWGSIY 360

Query: 361 HLYEPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDA 420
           H+YEPLF+GWPG+RLREKAMKIAMEHIHYEDEN           +LNMLCCWVEDPYSDA
Sbjct: 361 HVYEPLFNGWPGRRLREKAMKIAMEHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDA 420

Query: 421 FKFHLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVK 480
           FKFHLQR+PDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFG TL+KAHHFVK
Sbjct: 421 FKFHLQRIPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGSTLRKAHHFVK 480

Query: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540
           HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP
Sbjct: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540

Query: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600
           LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT
Sbjct: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600

Query: 601 MEALMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660
           MEAL LFKKLHPGHRTKEIDAA+AKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV
Sbjct: 601 MEALALFKKLHPGHRTKEIDAALAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660

Query: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMA 720
           AAGRTYNNCVAIRKACHFLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MA
Sbjct: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMA 720

Query: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 732
           LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL
Sbjct: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 780

BLAST of PI0013216 vs. NCBI nr
Match: XP_008459938.1 (PREDICTED: cucurbitadienol synthase [Cucumis melo])

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 671/784 (85.59%), Postives = 689/784 (87.88%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC       ENENDDDEAI VAN+S   
Sbjct: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFC-----SGENENDDDEAIAVANNS--- 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQVRSFL---------------------- 120
                 +FENAR HFRNNRFHRKQSSDLFLAIQ    +                      
Sbjct: 61  ----ASKFENARNHFRNNRFHRKQSSDLFLAIQCEKEIIRNGAKNEGTTKVKEGEDVKKE 120

Query: 121 ---STFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC 180
              +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC
Sbjct: 121 AVKNTLERALSFYSAVQTSDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMC 180

Query: 181 RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGG 240
           RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGE ADGGE GAMTKARSWIL RGG
Sbjct: 181 RYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEAADGGEHGAMTKARSWILERGG 240

Query: 241 ATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYG 300
           ATAITSWGK            GNNPLPPEFWLLPY LPFHPGRMWCHCRMVYLPMSYLYG
Sbjct: 241 ATAITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYSLPFHPGRMWCHCRMVYLPMSYLYG 300

Query: 301 KRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYE 360
           KRFVGPITPIVLSLRKELYTIPYHEIDWN+SRNTCAKEDLYYPHPKMQD+LWGSIYH+YE
Sbjct: 301 KRFVGPITPIVLSLRKELYTIPYHEIDWNRSRNTCAKEDLYYPHPKMQDILWGSIYHVYE 360

Query: 361 PLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFH 420
           PLFSGWPGKRLREKAMKIAMEHIHYEDEN           +LNMLCCWVEDPYSDAFKFH
Sbjct: 361 PLFSGWPGKRLREKAMKIAMEHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFH 420

Query: 421 LQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQI 480
           LQR+PDYLWLAEDGMRMQGYNGSQLWDTAFSIQAI+STKLIDTFG TL+KAHHFVKHSQI
Sbjct: 421 LQRIPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAIISTKLIDTFGPTLRKAHHFVKHSQI 480

Query: 481 QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN 540
           QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN
Sbjct: 481 QEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKN 540

Query: 541 RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL 600
           RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL
Sbjct: 541 RLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEAL 600

Query: 601 MLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGR 660
            LFKKLHPGHRTKEIDAA+AKAANFLENMQ+TDGSWYGCWGVCFTYAGWFGIKGLVAAGR
Sbjct: 601 ALFKKLHPGHRTKEIDAAIAKAANFLENMQKTDGSWYGCWGVCFTYAGWFGIKGLVAAGR 660

Query: 661 TYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEA 720
           TYNNCVAIRKAC+FLLSKELPGGGWG        +VYTNLEGNKPHLVNTAWVMMALIEA
Sbjct: 661 TYNNCVAIRKACNFLLSKELPGGGWGESYLSCQNKVYTNLEGNKPHLVNTAWVMMALIEA 720

Query: 721 GQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS 730
           GQGERDPAPLHRAARLLINSQLE+GDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS
Sbjct: 721 GQGERDPAPLHRAARLLINSQLESGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYS 772

BLAST of PI0013216 vs. NCBI nr
Match: AJR21209.1 (cucurbitadienol synthase 1 [Citrullus colocynthis])

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 644/784 (82.14%), Postives = 674/784 (85.97%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGEKEEKW+KSISNHLGRQVWEFC      + N               HL
Sbjct: 1   MWRLKVGAESVGEKEEKWLKSISNHLGRQVWEFCAHQPTASPN---------------HL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ-----------------------VRSF 120
                +Q +NAR HFRNNRFHRKQSSDLFLAIQ                         + 
Sbjct: 61  -----QQIDNARNHFRNNRFHRKQSSDLFLAIQNEKEIANVTKGGGIKVKEEEDVRKETV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY
Sbjct: 121 KNTVERALSFYSAIQTNDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILDRGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITPIVLSLRKELYTIPYHEIDWN+SRNTCAKEDLYYPHPKMQD+LWGSIYHLYEPL
Sbjct: 301 FVGPITPIVLSLRKELYTIPYHEIDWNRSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA+++AM+HIHYEDEN           +LNMLCCWVEDPYSDAFKFHLQ
Sbjct: 361 FTRWPGKRLREKALQMAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RVPDYLW+AEDGMRMQGYNGSQLWDTAFS+QAI+STKLID+FG TLKKAH FVK SQIQ+
Sbjct: 421 RVPDYLWVAEDGMRMQGYNGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQ 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK+RL
Sbjct: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDY YVECTSATMEAL L
Sbjct: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID AVA+AANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDIAVARAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           N+CVAIRKAC FLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MALIEAGQ
Sbjct: 661 NSCVAIRKACDFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            ERDPAPLHRAARLLINSQLENGDFPQ+EIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 AERDPAPLHRAARLLINSQLENGDFPQEEIMGVFNKNCMITYAAYRNIFPIWALGEYFHR 764

BLAST of PI0013216 vs. NCBI nr
Match: AJR21210.1 (cucurbitadienol synthase 2 [Citrullus colocynthis])

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 644/784 (82.14%), Postives = 673/784 (85.84%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLKVG ESVGEKEEKW+KSISNHLGRQVWEFC +    + N               HL
Sbjct: 1   MWRLKVGAESVGEKEEKWLKSISNHLGRQVWEFCADQPTASPN---------------HL 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ-----------------------VRSF 120
                +Q +NARKHFRNNRFHRKQSSDLFLAIQ                         + 
Sbjct: 61  -----QQIDNARKHFRNNRFHRKQSSDLFLAIQNEKEIANGTKGGGIKVKEEEDVRKETV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            +T      +Y +    DGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY
Sbjct: 121 KNTVERALSFYSAIQTNDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG+STMFGSALNYVALRLLGEDADGGEGGAMTKAR WIL RGGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGTSTMFGSALNYVALRLLGEDADGGEGGAMTKARGWILDRGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
           AITSWGK            GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 AITSWGKLWLSVLGVYEWSGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQD+LWGSIYHLYEPL
Sbjct: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDILWGSIYHLYEPL 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
           F+ WPGKRLREKA+++AM+HIHYEDEN           +LNMLCCWVEDPYSDAFKFHLQ
Sbjct: 361 FTRWPGKRLREKALQMAMKHIHYEDENSRYICLGPVNKVLNMLCCWVEDPYSDAFKFHLQ 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           RVPDYLW+AEDGMRMQGYNGSQLWDTAFS+QAI+STKLID+FG TLKKAH FVK SQIQ+
Sbjct: 421 RVPDYLWIAEDGMRMQGYNGSQLWDTAFSVQAIISTKLIDSFGTTLKKAHDFVKDSQIQQ 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           D PGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEK+RL
Sbjct: 481 DFPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKSRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
           CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDY YVECTSATMEAL L
Sbjct: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYPYVECTSATMEALTL 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           FKKLHPGHRTKEID AVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY
Sbjct: 601 FKKLHPGHRTKEIDTAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
           + CVAIRKAC FLLSKELPGGGWG        +VYTNLEGN+PHLVNTAWV+MALIEAGQ
Sbjct: 661 STCVAIRKACDFLLSKELPGGGWGESYLSCQNKVYTNLEGNRPHLVNTAWVLMALIEAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            ERDPAPLHRAARLLINSQLENGDFPQ+EIMGVFNKNCMITYAAYRNIFPIWALGEY HR
Sbjct: 721 AERDPAPLHRAARLLINSQLENGDFPQEEIMGVFNKNCMITYAAYRNIFPIWALGEYFHR 764

BLAST of PI0013216 vs. TAIR 10
Match: AT2G07050.1 (cycloartenol synthase 1 )

HSP 1 Score: 1005.7 bits (2599), Expect = 1.8e-293
Identity = 478/784 (60.97%), Postives = 570/784 (72.70%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MW+LK     + E    W+++ +NH+GRQ WEF     + N    +D  A+         
Sbjct: 1   MWKLK-----IAEGGSPWLRTTNNHVGRQFWEF-----DPNLGTPEDLAAV--------- 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQV-----------------------RSF 120
                   E ARK F +NRF +K S+DL + +Q                           
Sbjct: 61  --------EEARKSFSDNRFVQKHSADLLMRLQFSRENLISPVLPQVKIEDTDDVTEEMV 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            +T      +Y +   +DG+W  D GGPMFLLPGL+I L +TG LN+VLS+ H+QEM RY
Sbjct: 121 ETTLKRGLDFYSTIQAHDGHWPGDYGGPMFLLPGLIITLSITGALNTVLSEQHKQEMRRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           +YNHQNEDGGWGLHIEG STMFGS LNYV LRLLGE  + G+G  M K R WIL  GGAT
Sbjct: 181 LYNHQNEDGGWGLHIEGPSTMFGSVLNYVTLRLLGEGPNDGDGD-MEKGRDWILNHGGAT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
            ITSWGK            GNNPLPPE WLLPY LP HPGRMWCHCRMVYLPMSYLYGKR
Sbjct: 241 NITSWGKMWLSVLGAFEWSGNNPLPPEIWLLPYFLPIHPGRMWCHCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPIT  VLSLRKEL+T+PYHE++WN++RN CAKEDLYYPHP +QD+LW S++ + EP+
Sbjct: 301 FVGPITSTVLSLRKELFTVPYHEVNWNEARNLCAKEDLYYPHPLVQDILWASLHKIVEPV 360

Query: 361 FSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHLQ 420
              WPG  LREKA++ A+EHIHYEDEN           +LNMLCCWVEDP S+AFK HL 
Sbjct: 361 LMRWPGANLREKAIRTAIEHIHYEDENTRYICIGPVNKVLNMLCCWVEDPNSEAFKLHLP 420

Query: 421 RVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQE 480
           R+ D+LWLAEDGM+MQGYNGSQLWDT F+IQAIL+T L++ +G  L+KAH FVK+SQ+ E
Sbjct: 421 RIHDFLWLAEDGMKMQGYNGSQLWDTGFAIQAILATNLVEEYGPVLEKAHSFVKNSQVLE 480

Query: 481 DCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNRL 540
           DCPGD N W+RHI KGAWPFST DHGW ISDCTAEGLKA+L+LSK+P  IVGEP++  RL
Sbjct: 481 DCPGDLNYWYRHISKGAWPFSTADHGWPISDCTAEGLKAALLLSKVPKAIVGEPIDAKRL 540

Query: 541 CDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALML 600
            +AVNV++SLQN +GG A+YELTRSYPWLELINPAETFGDIVIDY YVECTSA ++AL+ 
Sbjct: 541 YEAVNVIISLQNADGGLATYELTRSYPWLELINPAETFGDIVIDYPYVECTSAAIQALIS 600

Query: 601 FKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRTY 660
           F+KL+PGHR KE+D  + KA  F+E++Q  DGSWYG W VCFTY  WFG+KGLVA G+T 
Sbjct: 601 FRKLYPGHRKKEVDECIEKAVKFIESIQAADGSWYGSWAVCFTYGTWFGVKGLVAVGKTL 660

Query: 661 NNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAGQ 720
            N   + KAC FLLSK+ P GGWG        +VY+NL+GN+ H+VNTAW M+ALI AGQ
Sbjct: 661 KNSPHVAKACEFLLSKQQPSGGWGESYLSCQDKVYSNLDGNRSHVVNTAWAMLALIGAGQ 720

Query: 721 GERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSHR 732
            E D  PLHRAAR LIN+Q+ENGDFPQQEIMGVFN+NCMITYAAYRNIFPIWALGEY  +
Sbjct: 721 AEVDRKPLHRAARYLINAQMENGDFPQQEIMGVFNRNCMITYAAYRNIFPIWALGEYRCQ 756

BLAST of PI0013216 vs. TAIR 10
Match: AT3G45130.1 (lanosterol synthase 1 )

HSP 1 Score: 905.6 bits (2339), Expect = 2.6e-263
Identity = 442/789 (56.02%), Postives = 544/789 (68.95%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLK+   S G++E     S++ H+GRQ WE+        +N+    E           
Sbjct: 1   MWRLKL---SEGDEE-----SVNQHVGRQFWEY--------DNQFGTSE----------- 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ--------------------------- 120
              +R    + R +F  NRF  K SSDL    Q                           
Sbjct: 61  ---ERHHINHLRSNFTLNRFSSKHSSDLLYRFQCWKEKGKGMERLPQVKVKEGEERLINE 120

Query: 121 --VRSFLSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHR 180
             V   L     FY   +S    DG W  D GGP+FLLP LVI LYVT VL+  L+  H+
Sbjct: 121 EVVNVTLRRSLRFYSILQSQ---DGFWPGDYGGPLFLLPALVIGLYVTEVLDGTLTAQHQ 180

Query: 181 QEMCRYIYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWIL 240
            E+ RY+YNHQN+DGGWGLH+EG+STMF + L+YVALRL+GE+ DGG+ GAM  ARSWI 
Sbjct: 181 IEIRRYLYNHQNKDGGWGLHVEGNSTMFCTVLSYVALRLMGEELDGGD-GAMESARSWIH 240

Query: 241 GRGGATAITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMS 300
             GGAT I SWGKF           GNNPLPPE WLLPY LPFHPGRMWCHCRMVYLPMS
Sbjct: 241 HHGGATFIPSWGKFWLSVLGAYEWSGNNPLPPELWLLPYSLPFHPGRMWCHCRMVYLPMS 300

Query: 301 YLYGKRFVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIY 360
           YLYG+RFV      +LSLR+ELYTIPYH IDW+ +RN CAKEDLYYPHPK+QDVLW  + 
Sbjct: 301 YLYGRRFVCRTNGTILSLRRELYTIPYHHIDWDTARNQCAKEDLYYPHPKIQDVLWSCLN 360

Query: 361 HLYEPLFSGWPGKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDA 420
              EPL   WP   LR  A++  M+HIHYED+N           +LNMLCCWVE   S+A
Sbjct: 361 KFGEPLLERWPLNNLRNHALQTVMQHIHYEDQNSHYICIGPVNKVLNMLCCWVESSNSEA 420

Query: 421 FKFHLQRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVK 480
           FK HL R+ DYLW+AEDGM+MQGYNGSQLWD   ++QAIL+T L+D +G  LKKAH+++K
Sbjct: 421 FKSHLSRIKDYLWVAEDGMKMQGYNGSQLWDVTLAVQAILATNLVDDYGLMLKKAHNYIK 480

Query: 481 HSQIQEDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEP 540
           ++QI++D  GDP +W+RH  KG W FST D+ W +SDCTAE LKA+L+LS++P  +VGEP
Sbjct: 481 NTQIRKDTSGDPGLWYRHPCKGGWGFSTGDNPWPVSDCTAEALKAALLLSQMPVNLVGEP 540

Query: 541 LEKNRLCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSAT 600
           + +  L DAVN +LSLQN+NGGFASYELTRSYP LE+INP+ETFGDI+IDY YVECTSA 
Sbjct: 541 MPEEHLVDAVNFILSLQNKNGGFASYELTRSYPELEVINPSETFGDIIIDYQYVECTSAA 600

Query: 601 MEALMLFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLV 660
           ++ L+LF  L+  ++ KEI  ++ KA  F+E  Q  DGSWYG WGVCFTYA WFGIKG++
Sbjct: 601 IQGLVLFTTLNSSYKRKEIVGSINKAVEFIEKTQLPDGSWYGSWGVCFTYATWFGIKGML 660

Query: 661 AAGRTYNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMA 720
           A+G+TY + + IRKAC FLLSK+L  GGWG        +VYTNL GNK H+VNT+W ++A
Sbjct: 661 ASGKTYESSLCIRKACGFLLSKQLCCGGWGESYLSCQNKVYTNLPGNKSHIVNTSWALLA 720

Query: 721 LIEAGQGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWAL 731
           LIEAGQ  RDP PLHR A+ LINSQ+E+GD+PQQEI+GVFN+NCMI+Y+AYRNIFPIWAL
Sbjct: 721 LIEAGQASRDPMPLHRGAKSLINSQMEDGDYPQQEILGVFNRNCMISYSAYRNIFPIWAL 755

BLAST of PI0013216 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 821.6 bits (2121), Expect = 4.9e-238
Identity = 394/782 (50.38%), Postives = 519/782 (66.37%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MW+LK+     G KEE ++ S +N LGRQ WEF            D D   V        
Sbjct: 1   MWKLKIAN---GNKEEPYLFSTNNFLGRQTWEF------------DPDAGTV-------- 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQV---RSFLSTFPFFYL----------- 120
              +    E AR+ F ++RF  K SSDL   +Q    + F    P   +           
Sbjct: 61  --EELAAVEEARRKFYDDRFRVKASSDLIWRMQFLKEKKFEQVIPPAKVEDANNITSEIA 120

Query: 121 ---------YYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
                    +  +    DG+W ++  GP+F LP LV  LYVTG L+ + ++ HR+E+ RY
Sbjct: 121 TNALRKGVNFLSALQASDGHWPAENAGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           IY HQNEDGGWGLHIEG+STMF + LNY+ +R+LGE  +GG G A  +AR WIL  GGAT
Sbjct: 181 IYCHQNEDGGWGLHIEGNSTMFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGAT 240

Query: 241 AITSWGK-----------FGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
            I SWGK            G+NP+PPEFW+LP  LP HP +MWC+CR+VY+PMSYLYGKR
Sbjct: 241 YIPSWGKTWLSILGVFDWSGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPI+P++L LR+E+Y  PY +I+WN++R+ CAKED Y PHP++QDV+W  +Y   EP 
Sbjct: 301 FVGPISPLILQLREEIYLQPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPF 360

Query: 361 FSGWP-GKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHL 420
            + WP  K LREKA+ +AM+HIHYEDEN            L ML CWVEDP    FK HL
Sbjct: 361 LACWPFNKLLREKALGVAMKHIHYEDENSRYITIGCVEKALCMLACWVEDPNGIHFKKHL 420

Query: 421 QRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQ 480
            R+ DYLW+AEDGM+MQ + GSQLWD+ F++QA++++ L++     L++ + F+K+SQ++
Sbjct: 421 LRISDYLWIAEDGMKMQSF-GSQLWDSGFALQALVASNLVNEIPDVLRRGYDFLKNSQVR 480

Query: 481 EDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNR 540
           E+  GD    +RHI KG+W FS RDHGW  SDCTAE  K  L+LS +P  IVG  ++  +
Sbjct: 481 ENPSGDFTNMYRHISKGSWTFSDRDHGWQASDCTAESFKCCLLLSMIPPDIVGPKMDPEQ 540

Query: 541 LCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALM 600
           L +AV +LLSLQ++NGG  ++E  R   WLEL+NP E F DIV+++ Y ECTS+ ++AL+
Sbjct: 541 LYEAVTILLSLQSKNGGVTAWEPARGQEWLELLNPTEVFADIVVEHEYNECTSSAIQALI 600

Query: 601 LFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRT 660
           LFK+L+P HRT+EI+ ++ KA  ++E++Q  DGSWYG WGVCFTY+ WFG+ GL AAG+T
Sbjct: 601 LFKQLYPNHRTEEINTSIKKAVQYIESIQMLDGSWYGSWGVCFTYSTWFGLGGLAAAGKT 660

Query: 661 YNNCVAIRKACHFLLSKELPGGGWGRVYTNL--------EGNKPHLVNTAWVMMALIEAG 720
           YNNC+A+RK  HFLL+ +   GGWG  Y +         EG + +LV T+W MM L+ AG
Sbjct: 661 YNNCLAMRKGVHFLLTTQKDNGGWGESYLSCPKKRYIPSEGERSNLVQTSWAMMGLLHAG 720

Query: 721 QGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSH 729
           Q ERDP+PLHRAA+LLINSQLENGDFPQQEI G F KNC++ YAAYRNIFP+WAL EY  
Sbjct: 721 QAERDPSPLHRAAKLLINSQLENGDFPQQEITGAFMKNCLLHYAAYRNIFPVWALAEYRR 756

BLAST of PI0013216 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 4.5e-231
Identity = 392/782 (50.13%), Postives = 498/782 (63.68%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MWRLK+G+   G  ++ ++ + +N  GRQ WEF  +  +  E                  
Sbjct: 1   MWRLKIGE---GNGDDPYLFTTNNFAGRQTWEFDPDGGSPEE------------------ 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQ-----------------------VRSF 120
               R     AR+ F +NRFH K SSDL   +Q                         + 
Sbjct: 61  ----RHSVVEARRIFYDNRFHVKASSDLLWRMQFLREKKFEQRIAPVKVEDSEKVTFETA 120

Query: 121 LSTFPFFYLYYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
            S       ++ +    DG+W ++  GP+F LP LV  LY+TG L+ V +  HR+E+ RY
Sbjct: 121 TSALRRGIHFFSALQASDGHWPAENAGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRY 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           IY HQ EDGGWGLHIEG STMF + LNY+ +R+LGE  DGG   A  +AR WIL  GG T
Sbjct: 181 IYCHQKEDGGWGLHIEGHSTMFCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVT 240

Query: 241 AITSWGK-----------FGNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
            I SWGK            G+NP+PPEFW+LP   P HP +MW +CRMVYLPMSYLYGKR
Sbjct: 241 YIPSWGKTWLSILGVFDWSGSNPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGPIT ++L LRKELY  PY EI+W K R+ CAKED YYP P +Q+++W S+Y   EP 
Sbjct: 301 FVGPITSLILQLRKELYLQPYEEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPF 360

Query: 361 FSGWP-GKRLREKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHL 420
            + WP  K LREKA+++AM+HIHYEDEN           +L ML CWVEDP  D FK HL
Sbjct: 361 LARWPFNKLLREKALQLAMKHIHYEDENSRYITIGCVEKVLCMLACWVEDPNGDYFKKHL 420

Query: 421 QRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQ 480
            R+ DYLW+AEDGM+MQ + GSQLWDT F++QA+L++ L       L++ H F+K+SQ+ 
Sbjct: 421 SRISDYLWMAEDGMKMQSF-GSQLWDTGFAMQALLASNLSSEISDVLRRGHEFIKNSQVG 480

Query: 481 EDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNR 540
           E+  GD    +RHI KGAW FS RDHGW +SDCTA GLK  L+ S L   IVG   +  R
Sbjct: 481 ENPSGDYKSMYRHISKGAWTFSDRDHGWQVSDCTAHGLKCCLLFSMLAPDIVGPKQDPER 540

Query: 541 LCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALM 600
           L D+VN+LLSLQ++NGG  ++E   +  WLEL+NP E F DIVI++ Y ECTS+ ++AL 
Sbjct: 541 LHDSVNILLSLQSKNGGMTAWEPAGAPKWLELLNPTEMFSDIVIEHEYSECTSSAIQALS 600

Query: 601 LFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRT 660
           LFK+L+P HRT EI A + KAA +LENMQ  DGSWYG WG+CFTY  WF + GL AAG+T
Sbjct: 601 LFKQLYPDHRTTEITAFIKKAAEYLENMQTRDGSWYGNWGICFTYGTWFALAGLAAAGKT 660

Query: 661 YNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAG 720
           +N+C AIRK   FLL+ +   GGWG        ++Y    G   ++V TAW +M LI +G
Sbjct: 661 FNDCEAIRKGVQFLLAAQKDNGGWGESYLSCSKKIYIAQVGEISNVVQTAWALMGLIHSG 720

Query: 721 QGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEYSH 729
           Q ERDP PLHRAA+L+INSQLE+GDFPQQ+  GVF KNC + YAAYRNI P+WAL EY  
Sbjct: 721 QAERDPIPLHRAAKLIINSQLESGDFPQQQATGVFLKNCTLHYAAYRNIHPLWALAEYRA 756

BLAST of PI0013216 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-227
Identity = 379/778 (48.71%), Postives = 501/778 (64.40%), Query Frame = 0

Query: 1   MWRLKVGKESVGEKEEKWIKSISNHLGRQVWEFCGENENENENENDDDEAIVVANSSKHL 60
           MW+LK+G+   G  E+ ++ S +N +GRQ WEF                        K  
Sbjct: 1   MWKLKIGE---GNGEDPYLFSSNNFVGRQTWEF----------------------DPKAG 60

Query: 61  LQPQRRQFENARKHFRNNRFHRKQSSDLFLAIQV---RSFLSTFPFFYL----------- 120
              +R   E+AR+++ +NR   K  SDL   +Q      F    P   +           
Sbjct: 61  TPEERAAVEDARRNYLDNRPRVKGCSDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYKNA 120

Query: 121 ---------YYKSSLIYDGNWASDLGGPMFLLPGLVIALYVTGVLNSVLSKHHRQEMCRY 180
                    +Y +    DG+W +++ G +F LP LV   Y+TG L  +    HR+EM R+
Sbjct: 121 TDALRRAVSFYSALQSSDGHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRH 180

Query: 181 IYNHQNEDGGWGLHIEGSSTMFGSALNYVALRLLGEDADGGEGGAMTKARSWILGRGGAT 240
           IY HQNEDGGWGLHIEG S MF + LNY+ LR+LGE  +GG   A  +AR WIL  GG T
Sbjct: 181 IYCHQNEDGGWGLHIEGKSVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVT 240

Query: 241 AITSWGKF-----------GNNPLPPEFWLLPYCLPFHPGRMWCHCRMVYLPMSYLYGKR 300
            I SWGK            G NP+PPE WLLP   P H G+  C+ RMVY+PMSYLYGKR
Sbjct: 241 YIPSWGKIWLSILGIYDWSGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKR 300

Query: 301 FVGPITPIVLSLRKELYTIPYHEIDWNKSRNTCAKEDLYYPHPKMQDVLWGSIYHLYEPL 360
           FVGP+TP+++ LRKEL+  PY EI+WNK+R  CAKED+ YPHP +QD+LW ++++  EP+
Sbjct: 301 FVGPLTPLIMLLRKELHLQPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPI 360

Query: 361 FSGWPGKRL-REKAMKIAMEHIHYEDEN-----------ILNMLCCWVEDPYSDAFKFHL 420
            + WP K+L REKA+++AMEHIHYEDEN           +L ML CW+E+P  D FK HL
Sbjct: 361 LTNWPLKKLVREKALRVAMEHIHYEDENSHYITIGCVEKVLCMLACWIENPNGDHFKKHL 420

Query: 421 QRVPDYLWLAEDGMRMQGYNGSQLWDTAFSIQAILSTKLIDTFGQTLKKAHHFVKHSQIQ 480
            R+PD++W+AEDG++MQ + GSQLWDT F+IQA+L+  L D     L+K H F+K SQ++
Sbjct: 421 ARIPDFMWVAEDGLKMQSF-GSQLWDTVFAIQALLACDLSDETDDVLRKGHSFIKKSQVR 480

Query: 481 EDCPGDPNVWFRHIHKGAWPFSTRDHGWLISDCTAEGLKASLMLSKLPSKIVGEPLEKNR 540
           E+  GD    +RHI KGAW  S RDHGW +SDCTAE LK  ++LS +P+++VG+ ++  +
Sbjct: 481 ENPSGDFKSMYRHISKGAWTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQ 540

Query: 541 LCDAVNVLLSLQNENGGFASYELTRSYPWLELINPAETFGDIVIDYSYVECTSATMEALM 600
           L D+VN+LLSLQ E GG  ++E  R+  WLEL+NP + F  ++ +  YVECTSA ++AL+
Sbjct: 541 LYDSVNLLLSLQGEKGGLTAWEPVRAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALV 600

Query: 601 LFKKLHPGHRTKEIDAAVAKAANFLENMQRTDGSWYGCWGVCFTYAGWFGIKGLVAAGRT 660
           LFK+L+P HRTKEI  ++ K   F+E+ Q  DGSW+G WG+CF YA WF + GL AAG+T
Sbjct: 601 LFKQLYPDHRTKEIIKSIEKGVQFIESKQTPDGSWHGNWGICFIYATWFALSGLAAAGKT 660

Query: 661 YNNCVAIRKACHFLLSKELPGGGWG--------RVYTNLEGNKPHLVNTAWVMMALIEAG 720
           Y +C+A+RK   FLL+ +   GGWG        + Y  LEGN+ +LV TAW MM LI AG
Sbjct: 661 YKSCLAVRKGVDFLLAIQEEDGGWGESHLSCPEQRYIPLEGNRSNLVQTAWAMMGLIHAG 720

Query: 721 QGERDPAPLHRAARLLINSQLENGDFPQQEIMGVFNKNCMITYAAYRNIFPIWALGEY 725
           Q ERDP PLHRAA+L+I SQLENGDFPQQEI+GVF   CM+ YA YRNIFP+WAL EY
Sbjct: 721 QAERDPTPLHRAAKLIITSQLENGDFPQQEILGVFMNTCMLHYATYRNIFPLWALAEY 752