PI0013205 (gene) Melon (PI 482460) v1

Overview
NamePI0013205
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGeneral transcription factor IIH subunit
Locationchr09: 3859810 .. 3862384 (+)
RNA-Seq ExpressionPI0013205
SyntenyPI0013205
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAATGGGGAAAATAGGCAATTGAATGGGGAAGCCGATGAAGAAGATGACGACGACGATGCCAATGGACTTGCTGCGTGGGAAAGGACTTATGCGGATGACAGGTCGTGGGAAGCCCTGCAAGAGGATGAGTCTGGACTCCTTCGCCCGATCGACAATAAGGCAATTTACCATGCCCAGTATCGAAGGCGCCTTCGTACCCTTTCTTCCTTAGCAACCACTGCTCGGATTCAGAAGGGTCTTATTCGCTATCTCTGTATCGTCATTGACTTCTCCAAGGTATTCTTTTTTCTTCCATGACTCTATTACTATATTAGTTTTCCTTTTTTTTTTTTTTTTGTGATAACTTACTACATTAGTAAATGTTTCAACTGTTTGATTTATGAGGTTCTGTGCCTTGGCCTCTCATCTTCTTATTGAGAAAAGGGATTTGTTCTAATGTTCATCAAGTTAAATGCATAGCATTGTTCCTGAAATGTATCATAACCATCAAATTGCATTGGTTTTTTCAGCTTACCTAAGAACTGTTATGTGGGATAAATTCGTTGTCTCCCCAATTGTTTGTTCTCTGATTATGTTTCAAAGGGTTTGGAGAAGCATCTCCACAGTTATTCCATAGAGGATTGCTACTCATTTAATCCAACTACTATGCAGTGAATGAAAAATGTGCTACCTTGAGTTTTTTTGGAGCAAGTATGATTTATGTTCATAGAAACTGTAAATCACTGGAGATAAATAACTTTGAACGGATGGTGATGGAATGGAGGTTTTGCTTTCTCTATCTCTCATTTTGCTTATTCCACCATGGGCAGGCTGCTACAGAAATGGATTTCCGACCAAGTCGAATGGCTGTTGTGGCAAAACACATAGAGGCTTTTGTCAGGGAATTCTTTGACCAAAATCCACTCAGCCAGATTGGTTTGGTGACTATAAAAGATGGAGTTGCTCATTGCTTAACAGATCTTGGTGGAAGTCCTGAATCTCATGTTAAAGCGTTAATGGGTAAACTGGAATGCTCAGGTGATGCTTCCTTGCAGAATGGTCTGGAACTTGTCCACAGCTATCTAAATCAAATTCCATCATATGGGCATAGAGAAGTTTTAATTTTATACTCTGCTCTTAATTCTTGTGATCCTGGGGACATCATGGAGACAGTTCAGAAATGCAAAACTTCTAAATAAGGTGTTCAGTTATTGGTCTTACTGCAGAAATTTTTATTTGCAGACATCTCTGCCAGGAAACTGGTGGCTCATACTCTGTCGCATTGGATGAGGTTAGTTCAATAGATGTGCGAATTTCTCAAAATTGAGAGAAATTGAAATGGCTATTTTTTTAGTTGACCAGCTAATTGAACCAACCATAAAGGAAGCTTAACAGAGAACACAGTTATGAAACAGAAGTTCAAATTTCAATTACTATAGCAAGATTTTATTTAATGTTCTTATATATAGAAGTTCTGTTTCCTAAATCTTAGTGGAATACTTGCCTTGCGTTTTATGTACAGGCTCACTTCAAAGAGTTGCTATTGGAGCATGCACCCCCACCCCCAGCAATTGCAGACTCTGCCATGCCTAATTTAATCAAGATGGGTTTTCCACAAAGAGCAGCAGAGAGTTCTATTGCAATATGTTCATGTCACAAAGAAGCTAAAGTTGGAGGGGGCTATACTTGCCCTCGATGCAAAGCACGGGTTTGTGAGCTTCCCACAGAGTGTCGAATTTGTGGACTGACACTTATCTCCTCACCTCATTTGGCTAGGTCATATCATCATCTCTTTCCAATTATACCATTTGATGAAGTCTCTGATAAAGTATTTCATGACCCACGACACCAACTGCCAAAAGTTTGCTTTGGCTGCCAAGAAAGCCTCATGAATCCTGGTAAGAACTCAATTAAACATGATATTTGAAGCTCTCAAATGAAACTTCTTTTTATGACATTTGTTGTCTTATAATATAGTGCTCATGCCAAATTAGTCAGCATTTTGTATCTGGATATCTTAGAATCAGATGATTTGAAGATTAAGAACATATTTATTTTCTTTACTACTAGGAGTTTGTTTATGCCCTGTAATGTTCGACTCCTAAAGCAAACAAAATGTTGTGTATAAGAGATCTTCCACGTTCCCTGTTCGTGTTTTGGTCATTCTTCAGTATTAATTATTGTAAGCACCAGTACTACAATCTGCTGAAACAAGCATAGGGAATTAAGTTGTTATCTATGTGATTTGATTTCGCCTTCTCTGTTGGGTGACTTCTTTTTATTGTATTGTTCATATGTGATTTTGTCTATGGTAATAGGAACTACTTATACTTGTGACATCCAAGTAAGAACAAATAAACATGGATGATTTGTTGTTACTTTATGATTTGCTGAATCTAATATTTTCACTCTCCATTGAAATGTACTTTTCATTTTAGGCACAGGTAATAGTCCAGGCATCCGTGTATCTTGCCCAAAGTGCAAACAACACTTCTGTCTTGATTGTGATATTTATATTCACGAGAGCTTGCACAATTGTCCTGGATGTGAGAGTTTCAGGCGTCCCAAGTTAGCGACTTCTGATGAATGA

mRNA sequence

ATGAACAATGGGGAAAATAGGCAATTGAATGGGGAAGCCGATGAAGAAGATGACGACGACGATGCCAATGGACTTGCTGCGTGGGAAAGGACTTATGCGGATGACAGGTCGTGGGAAGCCCTGCAAGAGGATGAGTCTGGACTCCTTCGCCCGATCGACAATAAGGCAATTTACCATGCCCAGTATCGAAGGCGCCTTCGTACCCTTTCTTCCTTAGCAACCACTGCTCGGATTCAGAAGGGTCTTATTCGCTATCTCTGTATCGTCATTGACTTCTCCAAGGCTGCTACAGAAATGGATTTCCGACCAAGTCGAATGGCTGTTGTGGCAAAACACATAGAGGCTTTTGTCAGGGAATTCTTTGACCAAAATCCACTCAGCCAGATTGGTTTGGTGACTATAAAAGATGGAGTTGCTCATTGCTTAACAGATCTTGGTGGAAGTCCTGAATCTCATGTTAAAGCGTTAATGGGTAAACTGGAATGCTCAGGTGATGCTTCCTTGCAGAATGGTCTGGAACTTGTCCACAGCTATCTAAATCAAATTCCATCATATGGGCATAGAGAAGTTTTAATTTTATACTCTGCTCTTAATTCTTTTATTGGTCTTACTGCAGAAATTTTTATTTGCAGACATCTCTGCCAGGAAACTGGTGGCTCATACTCTGTCGCATTGGATGAGGCTCACTTCAAAGAGTTGCTATTGGAGCATGCACCCCCACCCCCAGCAATTGCAGACTCTGCCATGCCTAATTTAATCAAGATGGGTTTTCCACAAAGAGCAGCAGAGAGTTCTATTGCAATATGTTCATGTCACAAAGAAGCTAAAGTTGGAGGGGGCTATACTTGCCCTCGATGCAAAGCACGGGTTTGTGAGCTTCCCACAGAGTGTCGAATTTGTGGACTGACACTTATCTCCTCACCTCATTTGGCTAGGTCATATCATCATCTCTTTCCAATTATACCATTTGATGAAGTCTCTGATAAAGTATTTCATGACCCACGACACCAACTGCCAAAAGTTTGCTTTGGCTGCCAAGAAAGCCTCATGAATCCTGGCACAGGTAATAGTCCAGGCATCCGTGTATCTTGCCCAAAGTGCAAACAACACTTCTGTCTTGATTGTGATATTTATATTCACGAGAGCTTGCACAATTGTCCTGGATGTGAGAGTTTCAGGCGTCCCAAGTTAGCGACTTCTGATGAATGA

Coding sequence (CDS)

ATGAACAATGGGGAAAATAGGCAATTGAATGGGGAAGCCGATGAAGAAGATGACGACGACGATGCCAATGGACTTGCTGCGTGGGAAAGGACTTATGCGGATGACAGGTCGTGGGAAGCCCTGCAAGAGGATGAGTCTGGACTCCTTCGCCCGATCGACAATAAGGCAATTTACCATGCCCAGTATCGAAGGCGCCTTCGTACCCTTTCTTCCTTAGCAACCACTGCTCGGATTCAGAAGGGTCTTATTCGCTATCTCTGTATCGTCATTGACTTCTCCAAGGCTGCTACAGAAATGGATTTCCGACCAAGTCGAATGGCTGTTGTGGCAAAACACATAGAGGCTTTTGTCAGGGAATTCTTTGACCAAAATCCACTCAGCCAGATTGGTTTGGTGACTATAAAAGATGGAGTTGCTCATTGCTTAACAGATCTTGGTGGAAGTCCTGAATCTCATGTTAAAGCGTTAATGGGTAAACTGGAATGCTCAGGTGATGCTTCCTTGCAGAATGGTCTGGAACTTGTCCACAGCTATCTAAATCAAATTCCATCATATGGGCATAGAGAAGTTTTAATTTTATACTCTGCTCTTAATTCTTTTATTGGTCTTACTGCAGAAATTTTTATTTGCAGACATCTCTGCCAGGAAACTGGTGGCTCATACTCTGTCGCATTGGATGAGGCTCACTTCAAAGAGTTGCTATTGGAGCATGCACCCCCACCCCCAGCAATTGCAGACTCTGCCATGCCTAATTTAATCAAGATGGGTTTTCCACAAAGAGCAGCAGAGAGTTCTATTGCAATATGTTCATGTCACAAAGAAGCTAAAGTTGGAGGGGGCTATACTTGCCCTCGATGCAAAGCACGGGTTTGTGAGCTTCCCACAGAGTGTCGAATTTGTGGACTGACACTTATCTCCTCACCTCATTTGGCTAGGTCATATCATCATCTCTTTCCAATTATACCATTTGATGAAGTCTCTGATAAAGTATTTCATGACCCACGACACCAACTGCCAAAAGTTTGCTTTGGCTGCCAAGAAAGCCTCATGAATCCTGGCACAGGTAATAGTCCAGGCATCCGTGTATCTTGCCCAAAGTGCAAACAACACTTCTGTCTTGATTGTGATATTTATATTCACGAGAGCTTGCACAATTGTCCTGGATGTGAGAGTTTCAGGCGTCCCAAGTTAGCGACTTCTGATGAATGA

Protein sequence

MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLNQIPSYGHREVLILYSALNSFIGLTAEIFICRHLCQETGGSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGGGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLPKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLATSDE
Homology
BLAST of PI0013205 vs. ExPASy Swiss-Prot
Match: Q9ZVN9 (General transcription factor IIH subunit 2 OS=Arabidopsis thaliana OX=3702 GN=GTF2H2 PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 4.6e-174
Identity = 298/412 (72.33%), Postives = 335/412 (81.31%), Query Frame = 0

Query: 7   RQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQYRRRL 66
           R+ + +  EE+DD+DA G+  WER Y DDRSWE LQEDESGLLRPIDN AIYHAQYRRRL
Sbjct: 5   RKRSNDEREEEDDEDAEGIGEWERAYVDDRSWEELQEDESGLLRPIDNSAIYHAQYRRRL 64

Query: 67  RTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFFDQNPL 126
           R LS+ A   RIQKGLIRYL IVIDFS+AA EMDFRPSRMA++AKH+EAF+REFFDQNPL
Sbjct: 65  RMLSAAAAGTRIQKGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPL 124

Query: 127 SQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLNQIPSYG 186
           SQIGLV+IK+GVAH LTDLGGSPE+H+KALMGKLE  GD+SLQN LELVH +LNQ+PSYG
Sbjct: 125 SQIGLVSIKNGVAHTLTDLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYG 184

Query: 187 HREVLILYSAL---------------------NSFIGLTAEIFICRHLCQETGGSYSVAL 246
           HREVLILYSAL                      S IGL+AE+FIC+HLCQETGG YSVA+
Sbjct: 185 HREVLILYSALCTCDPGDIMETIQKCKKSKLRCSVIGLSAEMFICKHLCQETGGLYSVAV 244

Query: 247 DEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGGGYTCPR 306
           DE H K+LLLEHAPPPPAIA+ A+ NLIKMGFPQRAAE S+AICSCHKE K+G GY CPR
Sbjct: 245 DEVHLKDLLLEHAPPPPAIAEFAIANLIKMGFPQRAAEGSMAICSCHKEVKIGAGYMCPR 304

Query: 307 CKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEV-SDKVFHDPRHQLPKVCFG 366
           CKARVC+LPTEC ICGLTL+SSPHLARSYHHLFPI PFDEV +    +D R +L K CFG
Sbjct: 305 CKARVCDLPTECTICGLTLVSSPHLARSYHHLFPIAPFDEVPALSSLNDNRRKLGKSCFG 364

Query: 367 CQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK 397
           CQ+SL+  G GN P   V+C KCK +FCLDCDIYIHESLHNCPGCES  RPK
Sbjct: 365 CQQSLI--GAGNKPVPCVTCRKCKHYFCLDCDIYIHESLHNCPGCESIHRPK 414

BLAST of PI0013205 vs. ExPASy Swiss-Prot
Match: O74995 (General transcription and DNA repair factor IIH subunit ssl1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ssl1 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 8.6e-80
Identity = 162/419 (38.66%), Postives = 230/419 (54.89%), Query Frame = 0

Query: 2   NNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQ 61
           +  E+ Q NG         D N    WE  Y   RSW+ +QED  G L  +    I   +
Sbjct: 13  SESEDEQKNGRVKVRSRKTDDNEGYTWEGEY--QRSWDIVQEDAEGSLVGVIAGLIQSGK 72

Query: 62  YRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFF 121
            +R LR       T  +Q+G+IR++ +V+D S +  E DF   R  +  K+   FV EFF
Sbjct: 73  RKRLLR------DTTPLQRGIIRHMVLVLDLSNSMEERDFHHKRFDLQIKYASEFVLEFF 132

Query: 122 DQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLNQ 181
           +QNP+SQ+ ++ + DG+AH +TDL G+P+SH++ L    +CSG+ SLQN LE+  + L+ 
Sbjct: 133 EQNPISQLSIIGVMDGIAHRITDLHGNPQSHIQKLKSLRDCSGNFSLQNALEMARASLSH 192

Query: 182 IPSYGHREVLILYSALNS---------------------FIGLTAEIFICRHLCQETGGS 241
           I S+G REVLI++ ++ S                      +GL AE+ IC+ +C +T  S
Sbjct: 193 IASHGTREVLIIFGSILSSDPGDIFKTIDALVHDSIRVRIVGLAAEVAICKEICNKTNSS 252

Query: 242 ----YSVALDEAHFKELLLEHA-PPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEA 301
               Y V + E HF+ELLLE   PP    A +   +L+ MGFP +  E   ++C+CH   
Sbjct: 253 TKNAYGVVISEQHFRELLLESTIPPATDSAKTTDASLVMMGFPSKVVEQLPSLCACH-SI 312

Query: 302 KVGGGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPR 361
              GG+ CPRCKA+VC LP EC  C L LI S HLARSYHHLFP+  + E+         
Sbjct: 313 PSRGGFHCPRCKAKVCTLPIECPSCSLVLILSTHLARSYHHLFPLKNWSEIPWSANPKST 372

Query: 362 HQLPKVCFGCQ----ESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCE 391
           H     CF CQ    +  ++P   ++  +R +CP CK HFCLDCD++ HE LH C GC+
Sbjct: 373 H-----CFACQLPFPKPPVSPFDESTSSMRYACPSCKNHFCLDCDVFAHEQLHECYGCQ 417

BLAST of PI0013205 vs. ExPASy Swiss-Prot
Match: Q86KZ2 (General transcription factor IIH subunit 2 OS=Dictyostelium discoideum OX=44689 GN=gtf2h2 PE=3 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 4.3e-79
Identity = 159/423 (37.59%), Postives = 246/423 (58.16%), Query Frame = 0

Query: 2   NNGENRQLNGEA-DEED--------DDDDANGLAAWERTYADDRSWEALQEDESGLLRPI 61
           NN +N++ N    D+ED        +D+D      WE  +  +++W  + EDE G LRP 
Sbjct: 8   NNAQNKRTNRSLYDDEDGPAHVLQTNDEDGTNKYKWENRF--EKTWLTIDEDEHG-LRPS 67

Query: 62  DNKAIYHAQYRRRLRTLSS---LATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVV 121
           + +        RRL+       L+   R+++G+ R+LC+++D SK  +  D +PSR  V+
Sbjct: 68  NQEE--RNTRNRRLKNKDRDGILSQDQRVRRGMQRHLCLILDLSKTLSNQDLKPSRYQVL 127

Query: 122 AKHIEAFVREFFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQ 181
            +++E F++EFFDQNP+SQ+ ++  K+  A  +++L G+   H++A+   +   G+ S+Q
Sbjct: 128 LQNVELFIKEFFDQNPISQLSIIITKNSKAEKISELSGNRLRHIQAMKDAIAMEGEPSIQ 187

Query: 182 NGLELVHSYLNQIPSYGHREVLILYSALN---------------------SFIGLTAEIF 241
           N LE+  S L  +P YG REVL ++S+L                      SFI + AE++
Sbjct: 188 NSLEVALSSLCYVPKYGSREVLFIFSSLTTCDPSSLQKTIQSLKNESIRVSFIHMAAELY 247

Query: 242 ICRHLCQETGGSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAI 301
           IC+ + ++T G+  V L+E HF E L+    PPP I  +    L++MGFPQ+   +  + 
Sbjct: 248 ICKAIAEQTNGTSKVILNEEHFNESLMLKCQPPPTIGKTEAA-LVEMGFPQQITSTVPSP 307

Query: 302 CSCHKEAKVGGGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSD 361
           C CH++ K   GY CPRC  + CELPT+C+IC L+L+SSPHLARSYHHLF I  F+EV+ 
Sbjct: 308 CICHEKMKY-SGYICPRCGVKSCELPTDCQICNLSLVSSPHLARSYHHLFQIPLFNEVNW 367

Query: 362 KVFHDPRHQLPKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPG 392
           K  +         C GC    ++    +   +  SCP+C++ FCLDCD++IHESLHNCPG
Sbjct: 368 KELNK-----NVTCIGC----LSSSEKSILSLFFSCPRCQEIFCLDCDLFIHESLHNCPG 414

BLAST of PI0013205 vs. ExPASy Swiss-Prot
Match: Q2TBV5 (General transcription factor IIH subunit 2 OS=Bos taurus OX=9913 GN=GTF2H2 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.1e-78
Identity = 155/405 (38.27%), Postives = 229/405 (56.54%), Query Frame = 0

Query: 19  DDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQYRRRLRTLSSLATTARI 78
           D++      WE  Y  +R+WE L+EDESG L+      ++ A+ +R            ++
Sbjct: 2   DEEPERTKRWEGGY--ERTWEILKEDESGSLKATIEDILFKAKRKR------VFEHHGQV 61

Query: 79  QKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFFDQNPLSQIGLVTIKDGV 138
           + G++R+L +V+D S+   + D +P+R+    K +E FV E+FDQNP+SQIG++  K   
Sbjct: 62  RLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKR 121

Query: 139 AHCLTDLGGSPESHVKALMGKLE--CSGDASLQNGLELVHSYLNQIPSYGHREVLILYSA 198
           A  LT+L G+P  H+ +L   ++  C G+ SL N L +    L  +P +  REVLI++S+
Sbjct: 122 AEKLTELSGNPRKHITSLKKAVDMTCHGEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSS 181

Query: 199 LN---------------------SFIGLTAEIFICRHLCQETGGSYSVALDEAHFKELLL 258
           L                      S IGL+AE+ +C  L +ETGG+Y V LDE+H+KELL 
Sbjct: 182 LTTCDPSNIYDLIKSLKAAKIRVSIIGLSAEVRVCTALARETGGTYHVILDESHYKELLT 241

Query: 259 EHAPPPPAIADSAMPNLIKMGFPQRA--------AESSIAICSCHKEAKVG---GGYTCP 318
            H  PPPA ++S   +LI+MGFPQ          A+ S ++       + G   GGY CP
Sbjct: 242 HHVSPPPASSNSEC-SLIRMGFPQHTIASLSDQDAKPSFSMAHLDSNTEPGLTLGGYFCP 301

Query: 319 RCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLPKVCFG 378
           +C+A+ CELP EC+ICGLTL+S+PHLARSYHHLFP+  F E+  +      H   + C+ 
Sbjct: 302 QCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLE-----EHNGERFCYA 361

Query: 379 CQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGC 390
           CQ  L +            C  C+  FC+DCD+++H+SLH CPGC
Sbjct: 362 CQGELKDQHV-------YVCSVCQNVFCVDCDVFVHDSLHCCPGC 385

BLAST of PI0013205 vs. ExPASy Swiss-Prot
Match: Q13888 (General transcription factor IIH subunit 2 OS=Homo sapiens OX=9606 GN=GTF2H2 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.8e-78
Identity = 156/405 (38.52%), Postives = 231/405 (57.04%), Query Frame = 0

Query: 19  DDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQYRRRLRTLSSLATTARI 78
           D++      WE  Y  +R+WE L+EDESG L+      ++ A+ +R            ++
Sbjct: 2   DEEPERTKRWEGGY--ERTWEILKEDESGSLKATIEDILFKAKRKR------VFEHHGQV 61

Query: 79  QKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFFDQNPLSQIGLVTIKDGV 138
           + G++R+L +V+D S+   + D +P+R+    K +E FV E+FDQNP+SQIG++  K   
Sbjct: 62  RLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKR 121

Query: 139 AHCLTDLGGSPESHVKALMGKLE--CSGDASLQNGLELVHSYLNQIPSYGHREVLILYSA 198
           A  LT+L G+P  H+ +L   ++  C G+ SL N L +    L  +P +  REVLI++S+
Sbjct: 122 AEKLTELSGNPRKHITSLKKAVDMTCHGEPSLYNSLSIAMQTLKHMPGHTSREVLIIFSS 181

Query: 199 LN---------------------SFIGLTAEIFICRHLCQETGGSYSVALDEAHFKELLL 258
           L                      S IGL+AE+ +C  L +ETGG+Y V LDE+H+KELL 
Sbjct: 182 LTTCDPSNIYDLIKTLKAAKIRVSVIGLSAEVRVCTVLARETGGTYHVILDESHYKELLT 241

Query: 259 EHAPPPPAIADSAMPNLIKMGFPQRA--------AESSIAICSCHKEAKVG---GGYTCP 318
            H  PPPA + S+  +LI+MGFPQ          A+ S ++       + G   GGY CP
Sbjct: 242 HHVSPPPA-SSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDGNTEPGLTLGGYFCP 301

Query: 319 RCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLPKVCFG 378
           +C+A+ CELP EC+ICGLTL+S+PHLARSYHHLFP+  F E+  + ++  R      C+G
Sbjct: 302 QCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLEEYNGER-----FCYG 361

Query: 379 CQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGC 390
           CQ  L +            C  C+  FC+DCD+++H+SLH CPGC
Sbjct: 362 CQGELKDQHV-------YVCAVCQNVFCVDCDVFVHDSLHCCPGC 385

BLAST of PI0013205 vs. ExPASy TrEMBL
Match: A0A5A7UNG8 (General transcription factor IIH subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001400 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.3e-229
Identity = 393/423 (92.91%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLL PIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLCPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH+EAFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVEAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFR PKLAT
Sbjct: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRHPKLAT 420

BLAST of PI0013205 vs. ExPASy TrEMBL
Match: A0A0A0KPM4 (General transcription factor IIH subunit OS=Cucumis sativus OX=3659 GN=Csa_5G169080 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.0e-228
Identity = 391/423 (92.43%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH++AFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVDAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDG A+CLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGFANCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNP TGNSP IRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT
Sbjct: 361 KVCFGCQESLMNPSTGNSPSIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 420

BLAST of PI0013205 vs. ExPASy TrEMBL
Match: A0A1S3CUH8 (General transcription factor IIH subunit OS=Cucumis melo OX=3656 GN=LOC103504674 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.0e-228
Identity = 392/423 (92.67%), Postives = 396/423 (93.62%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLL PIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLCPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH+EAFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVEAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNPGT NSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFR PKLAT
Sbjct: 361 KVCFGCQESLMNPGTRNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRHPKLAT 420

BLAST of PI0013205 vs. ExPASy TrEMBL
Match: A0A6J1GC53 (General transcription factor IIH subunit OS=Cucurbita moschata OX=3662 GN=LOC111452792 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.6e-220
Identity = 377/424 (88.92%), Postives = 392/424 (92.45%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDAN-GLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH 60
           MNNGENR+LNGEA+EEDDDDDAN G+AAWERTYADDRSWEALQEDESGLLRPIDNKAI+H
Sbjct: 1   MNNGENRRLNGEAEEEDDDDDANGGMAAWERTYADDRSWEALQEDESGLLRPIDNKAIFH 60

Query: 61  AQYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVRE 120
           AQYRRRLR+LSSLATTARIQKGLIRYL +VIDFS+AA EMDFRPSRMAVVAKH+EAFVRE
Sbjct: 61  AQYRRRLRSLSSLATTARIQKGLIRYLYVVIDFSRAAAEMDFRPSRMAVVAKHVEAFVRE 120

Query: 121 FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYL 180
           FFDQNPLSQIGLVTIKDGVAH LTDLGGSPESHVKALMGKLECSG+ASLQNGL+LV  YL
Sbjct: 121 FFDQNPLSQIGLVTIKDGVAHSLTDLGGSPESHVKALMGKLECSGEASLQNGLDLVCGYL 180

Query: 181 NQIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETG 240
           NQIPSYGHREVLILYSALN                     S IGLTAE+FICRHLCQETG
Sbjct: 181 NQIPSYGHREVLILYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAELFICRHLCQETG 240

Query: 241 GSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG 300
           GSYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRA ESSIAICSCHKEAKVG
Sbjct: 241 GSYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAGESSIAICSCHKEAKVG 300

Query: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQL 360
           GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDK+FHDPRHQL
Sbjct: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKLFHDPRHQL 360

Query: 361 PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLA 403
           PKVCFGCQE+  NPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK A
Sbjct: 361 PKVCFGCQENFTNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKSA 420

BLAST of PI0013205 vs. ExPASy TrEMBL
Match: A0A6J1K980 (General transcription factor IIH subunit OS=Cucurbita maxima OX=3661 GN=LOC111493405 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.3e-219
Identity = 376/424 (88.68%), Postives = 391/424 (92.22%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDAN-GLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH 60
           MNNGENR+LNGEA+EEDDDDDAN G+AAWERTYADDRSWEALQEDESGLLRPIDNKAI+H
Sbjct: 1   MNNGENRRLNGEAEEEDDDDDANGGMAAWERTYADDRSWEALQEDESGLLRPIDNKAIFH 60

Query: 61  AQYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVRE 120
           AQYRRRLR+LSSLATTARIQKGLIRYL +VIDFS+AA EMDFRPSRMAVVAKH+EAFVRE
Sbjct: 61  AQYRRRLRSLSSLATTARIQKGLIRYLYVVIDFSRAAAEMDFRPSRMAVVAKHVEAFVRE 120

Query: 121 FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYL 180
           FFDQNPLSQIGLVTIKDGVAH LTDLGGSPESHVKALMGKLECSG+ASLQNGL+LV  YL
Sbjct: 121 FFDQNPLSQIGLVTIKDGVAHSLTDLGGSPESHVKALMGKLECSGEASLQNGLDLVCGYL 180

Query: 181 NQIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETG 240
           NQIPSYGHREVLILYSALN                     S IGLTAE+FICRHLCQETG
Sbjct: 181 NQIPSYGHREVLILYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAELFICRHLCQETG 240

Query: 241 GSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG 300
           GSY VALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRA ESSIAICSCHKEAKVG
Sbjct: 241 GSYLVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAGESSIAICSCHKEAKVG 300

Query: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQL 360
           GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDK+FHDPRHQL
Sbjct: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKLFHDPRHQL 360

Query: 361 PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLA 403
           PKVCFGCQE+  NPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK A
Sbjct: 361 PKVCFGCQENFTNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKSA 420

BLAST of PI0013205 vs. NCBI nr
Match: KAA0055121.1 (general transcription factor IIH subunit 2 [Cucumis melo var. makuwa] >TYK20738.1 general transcription factor IIH subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 804.3 bits (2076), Expect = 4.8e-229
Identity = 393/423 (92.91%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLL PIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLCPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH+EAFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVEAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFR PKLAT
Sbjct: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRHPKLAT 420

BLAST of PI0013205 vs. NCBI nr
Match: XP_004143721.1 (general transcription factor IIH subunit 2 [Cucumis sativus] >XP_031741116.1 general transcription factor IIH subunit 2 [Cucumis sativus] >KGN50372.1 hypothetical protein Csa_000481 [Cucumis sativus])

HSP 1 Score: 801.2 bits (2068), Expect = 4.1e-228
Identity = 391/423 (92.43%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH++AFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVDAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDG A+CLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGFANCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNP TGNSP IRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT
Sbjct: 361 KVCFGCQESLMNPSTGNSPSIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 420

BLAST of PI0013205 vs. NCBI nr
Match: XP_008467294.1 (PREDICTED: general transcription factor IIH subunit 2 [Cucumis melo])

HSP 1 Score: 801.2 bits (2068), Expect = 4.1e-228
Identity = 392/423 (92.67%), Postives = 396/423 (93.62%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHA 60
           MNNGENR+LNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLL PIDNKAIYHA
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLCPIDNKAIYHA 60

Query: 61  QYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREF 120
           QYRRRLRTLSSLATTARIQKGLIRYL IVIDFSKAATEMDFRPSRMAVVAKH+EAFVREF
Sbjct: 61  QYRRRLRTLSSLATTARIQKGLIRYLYIVIDFSKAATEMDFRPSRMAVVAKHVEAFVREF 120

Query: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180
           FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN
Sbjct: 121 FDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLN 180

Query: 181 QIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETGG 240
           QIPSYGHREVL+LYSALN                     S IGLTAEIFICRHLCQETGG
Sbjct: 181 QIPSYGHREVLVLYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETGG 240

Query: 241 SYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300
           SYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG
Sbjct: 241 SYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGG 300

Query: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360
           GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP
Sbjct: 301 GYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQLP 360

Query: 361 KVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLAT 403
           KVCFGCQESLMNPGT NSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFR PKLAT
Sbjct: 361 KVCFGCQESLMNPGTRNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRHPKLAT 420

BLAST of PI0013205 vs. NCBI nr
Match: XP_038874496.1 (general transcription factor IIH subunit 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 793.9 bits (2049), Expect = 6.5e-226
Identity = 389/424 (91.75%), Postives = 397/424 (93.63%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDAN-GLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH 60
           MNNGENR+LNGEADEEDDDDDAN GLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH
Sbjct: 1   MNNGENRRLNGEADEEDDDDDANGGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH 60

Query: 61  AQYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVRE 120
           AQYRRRLR+LSSLATTARIQKGLIRYL IVIDFS+AATEMDFRPSRMAVVAKH+EAFVRE
Sbjct: 61  AQYRRRLRSLSSLATTARIQKGLIRYLYIVIDFSRAATEMDFRPSRMAVVAKHVEAFVRE 120

Query: 121 FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYL 180
           FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVH +L
Sbjct: 121 FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHGFL 180

Query: 181 NQIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETG 240
           NQIPSYGHREVLILYSALN                     S IGLTAEIFICRHLCQETG
Sbjct: 181 NQIPSYGHREVLILYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAEIFICRHLCQETG 240

Query: 241 GSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG 300
           GSYS+ALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG
Sbjct: 241 GSYSIALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG 300

Query: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQL 360
           GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKV HDPR+QL
Sbjct: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVLHDPRNQL 360

Query: 361 PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLA 403
           PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK A
Sbjct: 361 PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKSA 420

BLAST of PI0013205 vs. NCBI nr
Match: XP_022949453.1 (general transcription factor IIH subunit 2 [Cucurbita moschata] >XP_023525764.1 general transcription factor IIH subunit 2 [Cucurbita pepo subsp. pepo] >KAG6607180.1 General transcription factor IIH subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 774.2 bits (1998), Expect = 5.3e-220
Identity = 377/424 (88.92%), Postives = 392/424 (92.45%), Query Frame = 0

Query: 1   MNNGENRQLNGEADEEDDDDDAN-GLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYH 60
           MNNGENR+LNGEA+EEDDDDDAN G+AAWERTYADDRSWEALQEDESGLLRPIDNKAI+H
Sbjct: 1   MNNGENRRLNGEAEEEDDDDDANGGMAAWERTYADDRSWEALQEDESGLLRPIDNKAIFH 60

Query: 61  AQYRRRLRTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVRE 120
           AQYRRRLR+LSSLATTARIQKGLIRYL +VIDFS+AA EMDFRPSRMAVVAKH+EAFVRE
Sbjct: 61  AQYRRRLRSLSSLATTARIQKGLIRYLYVVIDFSRAAAEMDFRPSRMAVVAKHVEAFVRE 120

Query: 121 FFDQNPLSQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYL 180
           FFDQNPLSQIGLVTIKDGVAH LTDLGGSPESHVKALMGKLECSG+ASLQNGL+LV  YL
Sbjct: 121 FFDQNPLSQIGLVTIKDGVAHSLTDLGGSPESHVKALMGKLECSGEASLQNGLDLVCGYL 180

Query: 181 NQIPSYGHREVLILYSALN---------------------SFIGLTAEIFICRHLCQETG 240
           NQIPSYGHREVLILYSALN                     S IGLTAE+FICRHLCQETG
Sbjct: 181 NQIPSYGHREVLILYSALNSCDPGDIMETVQKCKTSKIRCSVIGLTAELFICRHLCQETG 240

Query: 241 GSYSVALDEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVG 300
           GSYSVALDE+HFKELLLEHAPPPPAIADSAMPNLIKMGFPQRA ESSIAICSCHKEAKVG
Sbjct: 241 GSYSVALDESHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAGESSIAICSCHKEAKVG 300

Query: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKVFHDPRHQL 360
           GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDK+FHDPRHQL
Sbjct: 301 GGYTCPRCKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEVSDKLFHDPRHQL 360

Query: 361 PKVCFGCQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKLA 403
           PKVCFGCQE+  NPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK A
Sbjct: 361 PKVCFGCQENFTNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPKSA 420

BLAST of PI0013205 vs. TAIR 10
Match: AT1G05055.1 (general transcription factor II H2 )

HSP 1 Score: 612.1 bits (1577), Expect = 3.3e-175
Identity = 298/412 (72.33%), Postives = 335/412 (81.31%), Query Frame = 0

Query: 7   RQLNGEADEEDDDDDANGLAAWERTYADDRSWEALQEDESGLLRPIDNKAIYHAQYRRRL 66
           R+ + +  EE+DD+DA G+  WER Y DDRSWE LQEDESGLLRPIDN AIYHAQYRRRL
Sbjct: 5   RKRSNDEREEEDDEDAEGIGEWERAYVDDRSWEELQEDESGLLRPIDNSAIYHAQYRRRL 64

Query: 67  RTLSSLATTARIQKGLIRYLCIVIDFSKAATEMDFRPSRMAVVAKHIEAFVREFFDQNPL 126
           R LS+ A   RIQKGLIRYL IVIDFS+AA EMDFRPSRMA++AKH+EAF+REFFDQNPL
Sbjct: 65  RMLSAAAAGTRIQKGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPL 124

Query: 127 SQIGLVTIKDGVAHCLTDLGGSPESHVKALMGKLECSGDASLQNGLELVHSYLNQIPSYG 186
           SQIGLV+IK+GVAH LTDLGGSPE+H+KALMGKLE  GD+SLQN LELVH +LNQ+PSYG
Sbjct: 125 SQIGLVSIKNGVAHTLTDLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYG 184

Query: 187 HREVLILYSAL---------------------NSFIGLTAEIFICRHLCQETGGSYSVAL 246
           HREVLILYSAL                      S IGL+AE+FIC+HLCQETGG YSVA+
Sbjct: 185 HREVLILYSALCTCDPGDIMETIQKCKKSKLRCSVIGLSAEMFICKHLCQETGGLYSVAV 244

Query: 247 DEAHFKELLLEHAPPPPAIADSAMPNLIKMGFPQRAAESSIAICSCHKEAKVGGGYTCPR 306
           DE H K+LLLEHAPPPPAIA+ A+ NLIKMGFPQRAAE S+AICSCHKE K+G GY CPR
Sbjct: 245 DEVHLKDLLLEHAPPPPAIAEFAIANLIKMGFPQRAAEGSMAICSCHKEVKIGAGYMCPR 304

Query: 307 CKARVCELPTECRICGLTLISSPHLARSYHHLFPIIPFDEV-SDKVFHDPRHQLPKVCFG 366
           CKARVC+LPTEC ICGLTL+SSPHLARSYHHLFPI PFDEV +    +D R +L K CFG
Sbjct: 305 CKARVCDLPTECTICGLTLVSSPHLARSYHHLFPIAPFDEVPALSSLNDNRRKLGKSCFG 364

Query: 367 CQESLMNPGTGNSPGIRVSCPKCKQHFCLDCDIYIHESLHNCPGCESFRRPK 397
           CQ+SL+  G GN P   V+C KCK +FCLDCDIYIHESLHNCPGCES  RPK
Sbjct: 365 CQQSLI--GAGNKPVPCVTCRKCKHYFCLDCDIYIHESLHNCPGCESIHRPK 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVN94.6e-17472.33General transcription factor IIH subunit 2 OS=Arabidopsis thaliana OX=3702 GN=GT... [more]
O749958.6e-8038.66General transcription and DNA repair factor IIH subunit ssl1 OS=Schizosaccharomy... [more]
Q86KZ24.3e-7937.59General transcription factor IIH subunit 2 OS=Dictyostelium discoideum OX=44689 ... [more]
Q2TBV52.1e-7838.27General transcription factor IIH subunit 2 OS=Bos taurus OX=9913 GN=GTF2H2 PE=2 ... [more]
Q138882.8e-7838.52General transcription factor IIH subunit 2 OS=Homo sapiens OX=9606 GN=GTF2H2 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7UNG82.3e-22992.91General transcription factor IIH subunit OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0KPM42.0e-22892.43General transcription factor IIH subunit OS=Cucumis sativus OX=3659 GN=Csa_5G169... [more]
A0A1S3CUH82.0e-22892.67General transcription factor IIH subunit OS=Cucumis melo OX=3656 GN=LOC103504674... [more]
A0A6J1GC532.6e-22088.92General transcription factor IIH subunit OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1K9801.3e-21988.68General transcription factor IIH subunit OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
Match NameE-valueIdentityDescription
KAA0055121.14.8e-22992.91general transcription factor IIH subunit 2 [Cucumis melo var. makuwa] >TYK20738.... [more]
XP_004143721.14.1e-22892.43general transcription factor IIH subunit 2 [Cucumis sativus] >XP_031741116.1 gen... [more]
XP_008467294.14.1e-22892.67PREDICTED: general transcription factor IIH subunit 2 [Cucumis melo][more]
XP_038874496.16.5e-22691.75general transcription factor IIH subunit 2 isoform X1 [Benincasa hispida][more]
XP_022949453.15.3e-22088.92general transcription factor IIH subunit 2 [Cucurbita moschata] >XP_023525764.1 ... [more]
Match NameE-valueIdentityDescription
AT1G05055.13.3e-17572.33general transcription factor II H2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004595TFIIH C1-like domainSMARTSM01047C1_4_2coord: 341..391
e-value: 1.8E-20
score: 84.0
IPR004595TFIIH C1-like domainPFAMPF07975C1_4coord: 342..391
e-value: 3.0E-19
score: 69.0
IPR007198Ssl1-likePFAMPF04056Ssl1coord: 89..199
e-value: 9.6E-44
score: 149.5
IPR007198Ssl1-likeCDDcd01453vWA_transcription_factor_IIH_typecoord: 81..242
e-value: 1.20718E-83
score: 251.481
IPR036465von Willebrand factor A-like domain superfamilyGENE3D3.40.50.410von Willebrand factor, type A domaincoord: 79..199
e-value: 5.5E-40
score: 138.9
coord: 200..243
e-value: 1.8E-11
score: 45.9
IPR036465von Willebrand factor A-like domain superfamilySUPERFAMILY53300vWA-likecoord: 81..198
IPR012170TFIIH subunit Ssl1/p44PIRSFPIRSF015919TFIIH_SSL1coord: 198..392
e-value: 8.1E-76
score: 253.4
coord: 1..200
e-value: 6.2E-64
score: 214.3
IPR012170TFIIH subunit Ssl1/p44TIGRFAMTIGR00622TIGR00622coord: 280..391
e-value: 2.8E-37
score: 125.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 318..396
e-value: 1.3E-20
score: 75.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR12695GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2coord: 17..199
coord: 199..389
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 315..390
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 364..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013205.1PI0013205.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006289 nucleotide-excision repair
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006281 DNA repair
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0000439 transcription factor TFIIH core complex
cellular_component GO:0005675 transcription factor TFIIH holo complex
molecular_function GO:0008270 zinc ion binding