PI0013145 (gene) Melon (PI 482460) v1

Overview
NamePI0013145
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiondisease resistance protein RGA2-like
Locationchr04: 7942133 .. 7944071 (-)
RNA-Seq ExpressionPI0013145
SyntenyPI0013145
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGGTGTGGCATGGGAGTTGGAGAATGAGTTGTCCCTATTGAAAGACAAGTTACATGATGCTGATTCAATCTTAGAAGACATCAATAGAAAGAAATCACACAATGACTCTGTGAAGCGATGGGTGCAAAAACTTGAAGATATCGTCCATGAAGCTGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGTACTGTGGAGCATACTGAGAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATGAGGAACATTACTGATACGTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGACTAGTTGGTGTGGAATCTGTCATAGAAACAGAGCTTGCTCTCAAACAGATTCGGGAGACAACCTCAATTCTTGACTTCCAAGTTGAAAGCAGGGAAGCTGAAGTTTTGGAGCTACTTAAATTGGTGATTGACTCTACCAATGAACATCATATGTCTGTGATATCCATTGTTGGAATGGGTGGTCTTGGCAAAACAACTTTGGCCAAGATGATCTTCAATCATGATGCCATTAAAAGACATTTTGATCAAACTATATGGGTTTGTGTGTCAAAACCATTTATTGTCACGAAATTTTGGAACAAATCTTTCAAGGTTTAACAATGACTTGTAGTGGGTTGGAATCCAATAAGGAGGCCTTGCTTGGGAGGCTTCGAAAGGAGATGCATGACAAGAAGTATTTTCTTGTGCTTGACGATGTTTGGGATAATGAGAAACACTTGTGGGACGAGCTTAGTGGCTGTTTGAAACATATTTCTGGAAAATCTGGAAATATTATTATGGTGACCACAAGGAATGTAGAGGTAGCAACAATGGTGGAGACAATTTCCATTCATCATCTAAAAAATTATCCGATGATCAATGTTGGGCATTGTTTAAAGAAAGTGCAAATGCTAATCAGCTGCCAATGAATGCGAAGTTGGAGAGTATAAAAACGGAGTTGGTTAAAAAAATGGGTGGTGTACCACTCGTTGCAAAAGTTTTAGGGGGGGCAGTCAAATTTGAAGAAGCTGAATTTAAAGAAGGAGATTATGAGACCTGGATGATGAAAGTTGAAAGCATTGCAAGGAACATTTCAATAGAGGACAAAGAATTTGTTTTGTCCATATTAAAATTAAGTGTGGATTCTTTACCAAATCCTGTGTTGAAGCAATGTTTTGCCTACTGTTCAAATTTTTCCCAAGACTATGATTTTCAGAAAGACGACCTAATTCAAATGTGGATAGCACAAAGATTTATTCAACCCCAACAAGAAAGAGAGAACTTAATAATGGAGGATATAGGAGAACAATACTTCAACTTCTTATTGTCTCGCTCCTTATTTCAAGATGTCACTAGGGATGAGAATAATAGAATTGTTACCTTTAAGATGCATGATCTAATACATGATATTGCTTGTGCAATTTCGTGCCATCAAAATATGGAATCAAATACTCCAAATAATTTGAGTCGAAAAAGCACAAGAAAGTTGCACATGTTGATTTTCGATGATGAAGTGATGGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAACTAAATAGGAGTCGAGCTCGAGATTTGTGGATTCCACTGGACAAGTTGATACATTTAAGATATCTTGATATTTCAGATTCTGATTTTAGGAACCGTCCTCAACAATCTCTTTCTCTACTTTATAATTTACAAACGTTAAAGCTTGGATTTATTGAGGGTGATCTACCGAAGAATTTGAGAAAATTGGTTAATCTAAGACATTTAGAATTTAGTTGTATTGATATGAAACAAATGCTTTACATATGTGCAATTTGA

mRNA sequence

ATGGTTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGGTGTGGCATGGGAGTTGGAGAATGAGTTGTCCCTATTGAAAGACAAGTTACATGATGCTGATTCAATCTTAGAAGACATCAATAGAAAGAAATCACACAATGACTCTGTGAAGCGATGGGTGCAAAAACTTGAAGATATCGTCCATGAAGCTGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGTACTGTGGAGCATACTGAGAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATGAGGAACATTACTGATACGTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGACTAGTTGGTGTGGAATCTGTCATAGAAACAGAGCTTGCTCTCAAACAGATTCGGGAGACAACCTCAATTCTTGACTTCCAAGTTGAAAGCAGGGAAGCTGAAGTTTTGGAGCTACTTAAATTGGTGATTGACTCTACCAATGAACATCATATGTCTGTGATATCCATTGTTGGAATGGGTGGTCTTGGCAAAACAACTTTGGCCAAGATGATCTTCAATCATGATGCCATTAAAAGACATTTTGATCAAACTATATGGGTTTGTGTGTCAAAACCATTTATTGTCACGAAATTTTGGAACAAATCTTTCAAGGAGGCCTTGCTTGGGAGGCTTCGAAAGGAGATGCATGACAAGAAGTATTTTCTTGTGCTTGACGATGTTTGGGATAATGAGAAACACTTGTGGGACGAGCTTAGTGGCTGTTTGAAACATATTTCTGGAAAATCTGGAAATATTATTATGGTGACCACAAGGAATGTAGAGGTAGCAACAATGGTGGAGACAATTTCCATTCATCATCTAAAAAATTATCCGATGATCAATCTGCCAATGAATGCGAAGTTGGAGAGTATAAAAACGGAGTTGGTTAAAAAAATGGGTGGTGTACCACTCGTTGCAAAAGTTTTAGGGGGGGCAGTCAAATTTGAAGAAGCTGAATTTAAAGAAGGAGATTATGAGACCTGGATGATGAAAGTTGAAAGCATTGCAAGGAACATTTCAATAGAGGACAAAGAATTTGTTTTGTCCATATTAAAATTAAGTGTGGATTCTTTACCAAATCCTGTGTTGAAGCAATGTTTTGCCTACTGTTCAAATTTTTCCCAAGACTATGATTTTCAGAAAGACGACCTAATTCAAATGTGGATAGCACAAAGATTTATTCAACCCCAACAAGAAAGAGAGAACTTAATAATGGAGGATATAGGAGAACAATACTTCAACTTCTTATTGTCTCGCTCCTTATTTCAAGATGTCACTAGGGATGAGAATAATAGAATTGTTACCTTTAAGATGCATGATCTAATACATGATATTGCTTGTGCAATTTCGTGCCATCAAAATATGGAATCAAATACTCCAAATAATTTGAGTCGAAAAAGCACAAGAAAGTTGCACATGTTGATTTTCGATGATGAAGTGATGGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAACTAAATAGGAGTCGAGCTCGAGATTTGTGGATTCCACTGGACAAGTTGATACATTTAAGATATCTTGATATTTCAGATTCTGATTTTAGGAACCGTCCTCAACAATCTCTTTCTCTACTTTATAATTTACAAACGTTAAAGCTTGGATTTATTGAGGGTGATCTACCGAAGAATTTGAGAAAATTGGTTAATCTAAGACATTTAGAATTTAGTTGTATTGATATGAAACAAATGCTTTACATATGTGCAATTTGA

Coding sequence (CDS)

ATGGTTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGGTGTGGCATGGGAGTTGGAGAATGAGTTGTCCCTATTGAAAGACAAGTTACATGATGCTGATTCAATCTTAGAAGACATCAATAGAAAGAAATCACACAATGACTCTGTGAAGCGATGGGTGCAAAAACTTGAAGATATCGTCCATGAAGCTGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGTACTGTGGAGCATACTGAGAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATGAGGAACATTACTGATACGTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGACTAGTTGGTGTGGAATCTGTCATAGAAACAGAGCTTGCTCTCAAACAGATTCGGGAGACAACCTCAATTCTTGACTTCCAAGTTGAAAGCAGGGAAGCTGAAGTTTTGGAGCTACTTAAATTGGTGATTGACTCTACCAATGAACATCATATGTCTGTGATATCCATTGTTGGAATGGGTGGTCTTGGCAAAACAACTTTGGCCAAGATGATCTTCAATCATGATGCCATTAAAAGACATTTTGATCAAACTATATGGGTTTGTGTGTCAAAACCATTTATTGTCACGAAATTTTGGAACAAATCTTTCAAGGAGGCCTTGCTTGGGAGGCTTCGAAAGGAGATGCATGACAAGAAGTATTTTCTTGTGCTTGACGATGTTTGGGATAATGAGAAACACTTGTGGGACGAGCTTAGTGGCTGTTTGAAACATATTTCTGGAAAATCTGGAAATATTATTATGGTGACCACAAGGAATGTAGAGGTAGCAACAATGGTGGAGACAATTTCCATTCATCATCTAAAAAATTATCCGATGATCAATCTGCCAATGAATGCGAAGTTGGAGAGTATAAAAACGGAGTTGGTTAAAAAAATGGGTGGTGTACCACTCGTTGCAAAAGTTTTAGGGGGGGCAGTCAAATTTGAAGAAGCTGAATTTAAAGAAGGAGATTATGAGACCTGGATGATGAAAGTTGAAAGCATTGCAAGGAACATTTCAATAGAGGACAAAGAATTTGTTTTGTCCATATTAAAATTAAGTGTGGATTCTTTACCAAATCCTGTGTTGAAGCAATGTTTTGCCTACTGTTCAAATTTTTCCCAAGACTATGATTTTCAGAAAGACGACCTAATTCAAATGTGGATAGCACAAAGATTTATTCAACCCCAACAAGAAAGAGAGAACTTAATAATGGAGGATATAGGAGAACAATACTTCAACTTCTTATTGTCTCGCTCCTTATTTCAAGATGTCACTAGGGATGAGAATAATAGAATTGTTACCTTTAAGATGCATGATCTAATACATGATATTGCTTGTGCAATTTCGTGCCATCAAAATATGGAATCAAATACTCCAAATAATTTGAGTCGAAAAAGCACAAGAAAGTTGCACATGTTGATTTTCGATGATGAAGTGATGGATTATAAGGTTTTGGACGACCTTGTTTGTTTGCGTGTTTTAAAACTAAATAGGAGTCGAGCTCGAGATTTGTGGATTCCACTGGACAAGTTGATACATTTAAGATATCTTGATATTTCAGATTCTGATTTTAGGAACCGTCCTCAACAATCTCTTTCTCTACTTTATAATTTACAAACGTTAAAGCTTGGATTTATTGAGGGTGATCTACCGAAGAATTTGAGAAAATTGGTTAATCTAAGACATTTAGAATTTAGTTGTATTGATATGAAACAAATGCTTTACATATGTGCAATTTGA

Protein sequence

MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLKLVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSFKEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVTTRNVEVATMVETISIHHLKNYPMINLPMNAKLESIKTELVKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLIFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSLLYNLQTLKLGFIEGDLPKNLRKLVNLRHLEFSCIDMKQMLYICAI
Homology
BLAST of PI0013145 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 2.1e-69
Identity = 204/627 (32.54%), Postives = 318/627 (50.72%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKL 69
           +Q VL  +  +   ++ + +  ++E   L        ++LED   K+ ++  ++ W+QKL
Sbjct: 6   IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65

Query: 70  EDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTL 129
               +E DD+LDE      + T     ++ +    + P      FR K+ K+M  +   L
Sbjct: 66  NAATYEVDDILDEY---KTKATRFLQSEYGRYHPKVIP------FRHKVGKRMDQVMKKL 125

Query: 130 YKHYCAASAFGLVGVESVIETELALKQIRETTSIL-DFQVESREAEVLELLKLVIDSTNE 189
                    F L   E +IE + A    RET S+L + QV  R+ E  E++K++I++ ++
Sbjct: 126 NAIAEERKKFHL--QEKIIERQAA---TRETGSVLTEPQVYGRDKEKDEIVKILINTASD 185

Query: 190 -HHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPF--------IVTKFW 249
              +SV+ I+GMGGLGKTTL++M+FN   +   F   IW+C+S  F        IV    
Sbjct: 186 AQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIE 245

Query: 250 NKSFKEALLGRLRKEMHD----KKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMV 309
            KS  +  L  L+K++ +    K+YFLVLDDVW+ ++H W  L   LK   G SG  ++ 
Sbjct: 246 GKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAFVLT 305

Query: 310 TTRNVEVATMVETISIHHLKNYPMINL-------------PMNAKLESIKTELVKKMGGV 369
           TTR  +V +++ T+  + L N    +               +N  L +I  E+VKK GGV
Sbjct: 306 TTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGV 365

Query: 370 PLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPV 429
           PL AK LGG ++F+  E        W    +S   N+  +D+  +L  L+LS   LP   
Sbjct: 366 PLAAKTLGGILRFKREE------REWEHVRDSPIWNLP-QDESSILPALRLSYHHLPLD- 425

Query: 430 LKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRSLF 489
           L+QCF YC+ F +D    K++LI  W+A  F+     + NL +ED+G + +N L  RS F
Sbjct: 426 LRQCFVYCAVFPKDTKMAKENLIAFWMAHGFL---LSKGNLELEDVGNEVWNELYLRSFF 485

Query: 490 QDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLIFDDEVMD 549
           Q++  +       FKMHDLIHD+A ++    N  S+    ++      +  + F + V  
Sbjct: 486 QEIEVESGK--TYFKMHDLIHDLATSL-FSANTSSSNIREINANYDGYMMSIGFAEVVSS 545

Query: 550 Y--KVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDS-DFRNRPQQSLSLLYNL 605
           Y   +L   V LRVL L  S    L   +  L+HLRYLD+S +   RN P++ L  L NL
Sbjct: 546 YSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKR-LCKLQNL 601

BLAST of PI0013145 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.6e-69
Identity = 202/628 (32.17%), Postives = 321/628 (51.11%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKL 69
           +Q +L  +  +   ++ + +  ++E   L        ++LED   K+ +N  ++ W+QKL
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65

Query: 70  EDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTL 129
               +E DD+LDE      + T     ++ +    + P      FR K+ K+M  +   L
Sbjct: 66  NAATYEVDDILDEY---KTKATRFSQSEYGRYHPKVIP------FRHKVGKRMDQVMKKL 125

Query: 130 YKHYCAASAFGLVGVESVIETELALKQIRETTSIL-DFQVESREAEVLELLKLVIDSTNE 189
                    F L   E ++E +      RET S+L + QV  R+ E  E++K++I++ ++
Sbjct: 126 KAIAEERKNFHL--HEKIVERQAVR---RETGSVLTEPQVYGRDKEKDEIVKILINNVSD 185

Query: 190 -HHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSFKEAL 249
             H+SV+ I+GMGGLGKTTLA+M+FN   +  HF   IW+CVS+ F   K   K+  E++
Sbjct: 186 AQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDF-DEKRLIKAIVESI 245

Query: 250 LGR--------------LRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNII 309
            GR              L++ ++ K+Y LVLDDVW+ ++  W  L   LK   G SG  +
Sbjct: 246 EGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGASV 305

Query: 310 MVTTRNVEVATMVETISIHHLKNYPMINL-------------PMNAKLESIKTELVKKMG 369
           + TTR  +V +++ T+  + L N    +               +N  L +I  E+VKK G
Sbjct: 306 LTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSG 365

Query: 370 GVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPN 429
           GVPL AK LGG + F+  E        W    +S   N+  +D+  +L  L+LS   LP 
Sbjct: 366 GVPLAAKTLGGILCFKREE------RAWEHVRDSPIWNLP-QDESSILPALRLSYHQLPL 425

Query: 430 PVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRS 489
             LKQCFAYC+ F +D   +K+ LI +W+A  F+     + N+ +ED+G++ +  L  RS
Sbjct: 426 D-LKQCFAYCAVFPKDAKMEKEKLISLWMAHGFL---LSKGNMELEDVGDEVWKELYLRS 485

Query: 490 LFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLIFDDEV 549
            FQ++   +      FKMHDLIHD+A ++    N  S+    +++ S   +  + F + V
Sbjct: 486 FFQEIEVKDGK--TYFKMHDLIHDLATSL-FSANTSSSNIREINKHSYTHMMSIGFAEVV 545

Query: 550 MDYKV--LDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSLLYN 605
             Y +  L+  + LRVL L  S    L   +  L+HLRYL++  S  R+ P+Q L  L N
Sbjct: 546 FFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQ-LCKLQN 601

BLAST of PI0013145 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-67
Identity = 206/638 (32.29%), Postives = 322/638 (50.47%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKL 69
           +Q +L  +  +   ++G+ +  E E   L        ++LED   K+    ++K W+QKL
Sbjct: 6   LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65

Query: 70  EDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTL 129
               +E DD+LD+   E  R         + V     P   +F +  K+ K+M+ + + L
Sbjct: 66  NVAAYEVDDILDDCKTEAARFK-------QAVLGRYHPRTITFCY--KVGKRMKEMMEKL 125

Query: 130 YKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLKLVIDSTN-E 189
                    F L   E +IE + A +Q      + + +V  RE E  E++K++I++ +  
Sbjct: 126 DAIAEERRNFHL--DERIIERQAARRQTGFV--LTEPKVYGREKEEDEIVKILINNVSYS 185

Query: 190 HHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPF--------IVTKFWN 249
             + V+ I+GMGGLGKTTLA+M+FN   I  HF+  IWVCVS  F        IV     
Sbjct: 186 EEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEG 245

Query: 250 KSFKEALLGRLRKEMHD----KKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVT 309
           KS  +  L  L+K++ +    K+YFLVLDDVW+ ++  WD L   LK   G SG  I++T
Sbjct: 246 KSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASILIT 305

Query: 310 TRNVEVATMVETISIHHLKNYPMIN-------------LPMNAKLESIKTELVKKMGGVP 369
           TR  ++ +++ T+ ++ L N    +                + KL  I  E+VKK GGVP
Sbjct: 306 TRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVP 365

Query: 370 LVAKVLGGAVKF--EEAEFKE-GDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPN 429
           L AK LGG ++F  EE+E++   D E W +           +D+  VL  L+LS   LP 
Sbjct: 366 LAAKTLGGLLRFKREESEWEHVRDSEIWNLP----------QDENSVLPALRLSYHHLPL 425

Query: 430 PVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRS 489
             L+QCFAYC+ F +D   +K+ LI +W+A  F+     + N+ +ED+G + +N L  RS
Sbjct: 426 D-LRQCFAYCAVFPKDTKIEKEYLIALWMAHSFL---LSKGNMELEDVGNEVWNELYLRS 485

Query: 490 LFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLI----- 549
            FQ++  +  +    FKMHDLIHD+A ++    +  S +   ++ K    +  ++     
Sbjct: 486 FFQEI--EVKSGKTYFKMHDLIHDLATSM-FSASASSRSIRQINVKDDEDMMFIVTNYKD 545

Query: 550 -----FDDEVMDY--KVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNR 605
                F + V  Y   +    V LRVL L+ S    L   +  L+HLRYLD+S +   + 
Sbjct: 546 MMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSL 605

BLAST of PI0013145 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.4e-67
Identity = 208/634 (32.81%), Postives = 322/634 (50.79%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKL 69
           +Q +L+ +  +  +++ + +  E E   L        ++L+D   K+  + +++ W+QKL
Sbjct: 6   LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65

Query: 70  EDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTL 129
               +E DD+L E   E +R        F + R           FR K+ ++M+ I + L
Sbjct: 66  NSAAYEVDDILGECKNEAIR--------FEQSRLGFY-HPGIINFRHKIGRRMKEIMEKL 125

Query: 130 YKHYCAASAFGLVGVESVIETELALKQIRETTSIL-DFQVESREAEVLELLKLVIDSTN- 189
                    F    +E + E + A    RET  +L + +V  R+ E  E++K++I++ N 
Sbjct: 126 DAISEERRKFHF--LEKITERQ-AAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNV 185

Query: 190 EHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPF--------IVTKFW 249
              + V  I+GMGGLGKTTLA+MIFN + + +HF+  IWVCVS  F        I+    
Sbjct: 186 AEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIE 245

Query: 250 NKSFKEALLGRLRKEMHD----KKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMV 309
             S     L   +K++ +    K+Y LVLDDVW+++   W +L   L    G  G  I+ 
Sbjct: 246 RSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGASILA 305

Query: 310 TTRNVEVATMVETISIHHLKNY-PMINLPM------------NAKLESIKTELVKKMGGV 369
           TTR  +V +++ T+  +HL N  P  +L +            N  L +I  E+VKK GGV
Sbjct: 306 TTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGV 365

Query: 370 PLVAKVLGGAVKF--EEAEFKE-GDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLP 429
           PL AK LGG ++F  EE+E++   D E W +           +D+  +L  L+LS   LP
Sbjct: 366 PLAAKTLGGLLRFKREESEWEHVRDNEIWSLP----------QDESSILPALRLSYHHLP 425

Query: 430 NPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSR 489
              L+QCFAYC+ F +D    K++LI +W+A  F+     + NL +ED+G + +N L  R
Sbjct: 426 LD-LRQCFAYCAVFPKDTKMIKENLITLWMAHGFL---LSKGNLELEDVGNEVWNELYLR 485

Query: 490 SLFQDVTRDENNRIVTFKMHDLIHDIACAI-----SCHQNMESNTPNNLSRKSTRKLHML 549
           S FQ++     N    FK+HDLIHD+A ++     SC    E N       K  +    +
Sbjct: 486 SFFQEIEAKSGN--TYFKIHDLIHDLATSLFSASASCGNIREINV------KDYKHTVSI 545

Query: 550 IFDDEVMDY--KVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQS 605
            F   V  Y   +L   V LRVL L+ S+   L   +  L+HLRYLD+S ++FR+ P++ 
Sbjct: 546 GFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPER- 602

BLAST of PI0013145 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.5e-51
Identity = 178/656 (27.13%), Postives = 322/656 (49.09%), Query Frame = 0

Query: 32  ENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKLEDIVHEADDLLDELVYEHLRRT 91
           EN L  L   L    ++L D   K+  N  V++WV +L D+V+ A+D LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V----EHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTLYKHYCAASAFGLVGVESV 151
           +      + + R++R  +S           +  ++  +T  L +    AS   ++G++ +
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGLKEL 155

Query: 152 IETELALKQIRETTSILD-FQVESREAEVLELLKLVI-DSTNEHHMSVISIVGMGGLGKT 211
             T +  KQ   TTS++D  +V  R+ +  E+++ +I ++  ++ ++V++IVG+GG+GKT
Sbjct: 156 --TAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKT 215

Query: 212 TLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSFK------------EALLGRLRK 271
           TL+++++N   ++ +F   +W  VS+ F V K   K ++            + L  +L++
Sbjct: 216 TLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKE 275

Query: 272 EMHDK--KYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVTTRNVEVATMVETISI 331
            +      + LVLDD+W+     WD L     H +   G+ I+VTTR+  VA+++  + +
Sbjct: 276 RLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCAVHV 335

Query: 332 HHLKN--------------YPMINLPMNAKLESIKTELVKKMGGVPLVAKVLGGAVKFEE 391
           H+L+               +      +N ++  +   +V K  G+PL  K LGG ++F  
Sbjct: 336 HNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRF-- 395

Query: 392 AEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDY 451
               EG    W   + S   ++   DK  +L +L++S   LP   LK+CFAYCS F + +
Sbjct: 396 ----EGKVIEWERVLSSRIWDLP-ADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGH 455

Query: 452 DFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRSLFQDVTRDENNRIVTFK 511
            F+KD ++ +W+A+ F+Q  +  +NL  E++G +YF+ L SRSL Q            + 
Sbjct: 456 AFEKDKVVLLWMAEGFLQQTRSSKNL--EELGNEYFSELESRSLLQKTK-------TRYI 515

Query: 512 MHDLIHDIA--------------CAISCHQN------MESNTPNNLSRKSTRKLHML--- 571
           MHD I+++A              C +   +       +  N    +  ++ R++  L   
Sbjct: 516 MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTF 575

Query: 572 -----------IFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLD---KLIHLRYLDI 615
                         D+++  K+L  L  LRVL L+  +   L  P D    + H R+LD+
Sbjct: 576 LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARL--PPDFFKNISHARFLDL 635

BLAST of PI0013145 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 5.1e-260
Identity = 487/629 (77.42%), Postives = 524/629 (83.31%), Query Frame = 0

Query: 7   TFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSH-NDSVKRW 66
           TF VQEVLK+IVKYGAEQI VAWELENE+SLLKDKLHDAD+ILEDINRKKSH  +SVKRW
Sbjct: 6   TFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRW 65

Query: 67  VQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNI 126
           V+KLEDIVHEADDLLDELVYEHLRRTVEHTEKF KV DSIS S NSFLFRRKMAKK++NI
Sbjct: 66  VEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAKKIKNI 125

Query: 127 TDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLKLVIDS 186
           TDTL +HYCAASAFGLVGVE+V E ELAL QIRETTSILDFQVE REAEVLELLKL IDS
Sbjct: 126 TDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLKLAIDS 185

Query: 187 TNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSF-- 246
           TNEHHMSVISIVGMGGLGKTTLAKMIFNH  I+ HFD+TIWVCVSKPFIVTK   K F  
Sbjct: 186 TNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQG 245

Query: 247 -----------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNI 306
                      KEALLGRLRKEM DK YFLVLDDVWDNEKHLWDEL GCLKHI+GK GN 
Sbjct: 246 LTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAGKPGNT 305

Query: 307 IMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTELVKKM 366
           IMVTTRN EVATMVE ISI+ LK                   LPMN+KLE +K ELV+KM
Sbjct: 306 IMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKELVRKM 365

Query: 367 GGVPLVAKVLGGAVKFEEAEFKEGDYE-TWMMKVESIARNISIEDKEFVLSILKLSVDSL 426
           GGVPLVAKVLGGAVKFEE E +E D+E +WM KVESI RNIS+EDK+FVLSILKLSVDSL
Sbjct: 366 GGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKLSVDSL 425

Query: 427 PNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERE-NLIMEDIGEQYFNFLL 486
           PNPVLKQC AYCSNFSQDYDFQKDDLI+MWIAQ FIQP Q R+ NL+MEDIGEQYFNFLL
Sbjct: 426 PNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLL 485

Query: 487 SRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLIFD 546
           SRS+FQDVTRD N RIV FKMHDL+HDIACAIS HQN+ESN PNNLS KS RKL  LI +
Sbjct: 486 SRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESN-PNNLSGKSVRKLRTLICN 545

Query: 547 DEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSLLY 606
           DEV++Y   +D+VCLRVLK+      DLWIP+DKLIHLRYLDIS+        +SLSLLY
Sbjct: 546 DEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESLSLLY 605

BLAST of PI0013145 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 1.2e-221
Identity = 424/640 (66.25%), Postives = 489/640 (76.41%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVLKKIV +GAEQI +AW LE ELS LK  L  A +IL DIN KKSH+ 
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV  WV++L DI++EADDLLDE+VYE +R+TVE T K RKVRDSISPSKNSFLF  KMAK
Sbjct: 61  SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           KM+ IT TLY+HYC AS  GLVG ES  E+E AL QIRETTSILDF+VE REAEVLE+LK
Sbjct: 121 KMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEILK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVIDST+E H+SVISIVGMGGLGKTTLAKM+FNHDAIK HFD+T+WVCVSKPFIV K   
Sbjct: 181 LVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            +EALL RLR+EM  KKYFLVLDDVWD E  LWDEL G LK+I+GK
Sbjct: 241 AIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIAGK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTEL 360
           SGN IMVTTR+VEVATMV+T+ I+HLK                   L MN+KLE+ K  L
Sbjct: 301 SGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTKNIL 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSV 420
           V+K+GGVPL+AKVLGGAVKFE     EG  E+WM K+ES ARNISIEDK+FVLSILKLSV
Sbjct: 361 VRKIGGVPLIAKVLGGAVKFE-----EGGSESWMAKIESFARNISIEDKDFVLSILKLSV 420

Query: 421 DSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNF 480
           +SLP+  LKQCFAYCSNF QDY+F KD+ IQMWIA+ FIQP+QERENL ME+IGE+Y NF
Sbjct: 421 ESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEYLNF 480

Query: 481 LLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLI 540
           LLSRSLF+D  +  + RIVTFK+HDL+HDIACAIS H  M+SN P + + KSTRKL  LI
Sbjct: 481 LLSRSLFEDAIK-YDGRIVTFKIHDLMHDIACAISNHHKMDSN-PISWNGKSTRKLRTLI 540

Query: 541 FDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSL 600
            ++E   +K+  D++CLRVL L       L   +DKLIHLRYLDIS+ +     + S+  
Sbjct: 541 CENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLRDSICA 600

Query: 601 LYNLQTLKLGFIEGDLPKNLRKLVNLRHLEF-SCIDMKQM 615
           LYNLQTLKLG+IE DLPKNLR LVNLRHLEF    DM QM
Sbjct: 601 LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQM 633

BLAST of PI0013145 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 8.4e-194
Identity = 380/631 (60.22%), Postives = 457/631 (72.42%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVL+KIVK  A+QIG+AW LE ELS L   L  A++IL DINR+K H+D
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV+ W++ L+D+VHEA DLLDELVYE LRR VE   K  KVR SIS S N F FRRKMAK
Sbjct: 61  SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVE-IGKMNKVRGSISRSYNLFSFRRKMAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           KM+++T+TLYKHYC AS  GLVG ES+   ++ LKQIRETTS L+F+V  RE EV  ++K
Sbjct: 121 KMKDVTETLYKHYCEASPLGLVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSNIVK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVIDS+NEHH S+I IVGMGGLGKTTLA+MIFNH+ IK HFD+TIW+CVS+PFI+     
Sbjct: 181 LVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIINILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            KE L+ +L++EMH KKYFLVLDDVW+ E  LWD+L  CLK I+ K
Sbjct: 241 GIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQITEK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTEL 360
           SGN I+VTTR++EVAT +ET+S HHLK                   L MN KL+ I+  L
Sbjct: 301 SGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIREIL 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSV 420
           VKK+GGVPLVAKVLGGA+KF      EGDYETW+ KV+S+ RNIS +DK+FVLSILKLSV
Sbjct: 361 VKKIGGVPLVAKVLGGAIKF------EGDYETWVKKVKSVVRNISKDDKDFVLSILKLSV 420

Query: 421 DSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNF 480
           D LP   LKQCFAYCSNFS+D  F K++LIQMWIAQ FIQ  +       EDIGE + NF
Sbjct: 421 DCLPLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNF 480

Query: 481 LLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLI 540
           LLSRSLFQDV +D N R+  FKMHDLIHDIACAIS H  +     +N SRKS RKL  LI
Sbjct: 481 LLSRSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLI 540

Query: 541 FDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSL 600
            +++V+ Y  L D V LRVL LN ++  +L   + KL HLRYLDIS    R  P +S++ 
Sbjct: 541 CNNQVI-YNELMDCVSLRVLVLNSAKTDNLLDSIGKLKHLRYLDISHYSIRVLP-ESIAK 600

Query: 601 LYNLQTLKLGFIEGDLPKNLRKLVNLRHLEF 607
           LYNLQTLK+G +E  LPKNLRK+V+LRHL+F
Sbjct: 601 LYNLQTLKIGRME-SLPKNLRKMVSLRHLKF 621

BLAST of PI0013145 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.9e-185
Identity = 375/643 (58.32%), Postives = 456/643 (70.92%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVL+KIVK  A+QIG+A  LE ELS L   L  A++IL DINRKKSH+D
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV+ WV+ L+D+VHEADDLLDELVYEHLRR VE   K  KVRDS S S N FLFR KMAK
Sbjct: 61  SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVE-IGKINKVRDSFSTSHNVFLFRLKMAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           K+++IT  L+KHYC AS  GLVG ES    ++ L QIR+TTS L+F+V  RE EV  ++K
Sbjct: 121 KIKDITQILHKHYCEASPLGLVGQESTEANQIVLSQIRDTTSNLNFEVVGREDEVSNIVK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVIDS+NEHHMS++ IVGMGGLGKTT A+ IFNHD IK HFD  IWVCVS+PFI+     
Sbjct: 181 LVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIINILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            K  LL +++KEMH K YFLVLDDVW+ E  LWD+L  CLK I+GK
Sbjct: 241 GIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQIAGK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTEL 360
           SGN I+VTTR++EVA +V T+  H LK                   L MN KLE I+  L
Sbjct: 301 SGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEIIRDML 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSV 420
           VKK+GGVPLVAKVLGGA KF      EGDYE W+ KVES+ RNIS +DK+FV SILKLSV
Sbjct: 361 VKKIGGVPLVAKVLGGATKF------EGDYERWVTKVESVVRNISNDDKDFVSSILKLSV 420

Query: 421 DSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQ-QEREN-LIMEDIGEQYF 480
           D LP   LKQCFAYCSNF++D  + K++LI++WIAQ FIQPQ QE +N +  EDIGE +F
Sbjct: 421 DCLPLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGEWHF 480

Query: 481 NFLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQN-MESNTPNNLSRKSTRKLH 540
           NFLLSRSLFQDVT D++ R + F MHDLIHDIACAIS HQ  +   + +N SRKS RKL 
Sbjct: 481 NFLLSRSLFQDVTMDKSERGIYFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLR 540

Query: 541 MLIFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQS 600
            LI +++V+ Y  L D V LRVL L+  +  +L   + KL HLRYL I++ ++ +   +S
Sbjct: 541 TLICNNQVI-YNELVDCVSLRVLVLHCEKTGNLLESIGKLKHLRYLSIANGNYVDVLPES 600

Query: 601 LSLLYNLQTLKLGFIEGDLPKNLRKLVNLRHLEFSC-IDMKQM 615
           + +LYNLQTLKL  ++  LPKN RK+V+LRHLEF    D +QM
Sbjct: 601 IVMLYNLQTLKLRHMK-SLPKNSRKMVSLRHLEFELKSDFRQM 634

BLAST of PI0013145 vs. ExPASy TrEMBL
Match: A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 6.4e-178
Identity = 356/640 (55.62%), Postives = 453/640 (70.78%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVLKK++K  AEQIGVAW L+ ELS L+ +L  A++IL DINR+K  ++
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV+ WV+ L+ +V++ADDLLD+LVYE LR+ VE  EK  KVR+ +SPS N FLFR KMAK
Sbjct: 61  SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVE-MEKINKVRNFLSPSNNPFLFRFKMAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIET-ELALKQIRETTSILDFQVESREAEVLELL 180
           +M+ I++TLY+HYC AS  G VG ES   T +  L+QIRETTSIL+F+V  RE EVL+++
Sbjct: 121 RMKAISETLYEHYCEASVLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVLKIM 180

Query: 181 KLVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFW 240
           KLVIDS++EH MS+I IVGMGGLGKTTLAKM+FNH+ IK HFD+TIWVCVSKPFI+    
Sbjct: 181 KLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIVNIL 240

Query: 241 ------------NKSFKEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISG 300
                         ++KE L  RL+KEM  KKYFLVLDDVW+ E  LWDEL  CL  I+ 
Sbjct: 241 EGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNEIAE 300

Query: 301 KSGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTE 360
           KSGN I+VTTR+  VAT+++T+  H L+                   L MN +LE+I+  
Sbjct: 301 KSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENIREV 360

Query: 361 LVKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLS 420
           +VK++GG+PLVAKVLGG  KF      EG+YE W+  VESI RNI  ++K+FVLS LKLS
Sbjct: 361 VVKRIGGIPLVAKVLGGTAKF------EGNYERWVTTVESIVRNILNDEKDFVLSTLKLS 420

Query: 421 VDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFN 480
           VDSLP   LKQCFA+CSNF +D +F K++LIQMWIAQ FIQ ++    ++ E+IGE YFN
Sbjct: 421 VDSLPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFN 480

Query: 481 FLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHML 540
           FLLSRSLFQDV++D +  I  FKMHDLIHD+AC +S +Q +E + P N S K  R L  L
Sbjct: 481 FLLSRSLFQDVSKDIDGTITHFKMHDLIHDVACIVS-NQKLELD-PRNWSEKGVRALRTL 540

Query: 541 IFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLS 600
           + ++  + +  L+D V LRVL LN    ++L   +  L HLRYLDI  S  R  P +S++
Sbjct: 541 VINNGAIIHNKLEDSVYLRVLVLNSDFIKNLPDSIGMLKHLRYLDIIGSRIRRLP-ESIT 600

Query: 601 LLYNLQTLKLGFIEGDLPKNLRKLVNLRHLEFSCIDMKQM 615
           +LYNLQ LKL     DLPKNLRKLV+LRHL F  I M++M
Sbjct: 601 MLYNLQILKLR-TRIDLPKNLRKLVSLRHLPFFYIHMEKM 629

BLAST of PI0013145 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 906.7 bits (2342), Expect = 1.1e-259
Identity = 487/629 (77.42%), Postives = 524/629 (83.31%), Query Frame = 0

Query: 7   TFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSH-NDSVKRW 66
           TF VQEVLK+IVKYGAEQI VAWELENE+SLLKDKLHDAD+ILEDINRKKSH  +SVKRW
Sbjct: 6   TFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRW 65

Query: 67  VQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNI 126
           V+KLEDIVHEADDLLDELVYEHLRRTVEHTEKF KV DSIS S NSFLFRRKMAKK++NI
Sbjct: 66  VEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAKKIKNI 125

Query: 127 TDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLKLVIDS 186
           TDTL +HYCAASAFGLVGVE+V E ELAL QIRETTSILDFQVE REAEVLELLKL IDS
Sbjct: 126 TDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLKLAIDS 185

Query: 187 TNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSF-- 246
           TNEHHMSVISIVGMGGLGKTTLAKMIFNH  I+ HFD+TIWVCVSKPFIVTK   K F  
Sbjct: 186 TNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQG 245

Query: 247 -----------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNI 306
                      KEALLGRLRKEM DK YFLVLDDVWDNEKHLWDEL GCLKHI+GK GN 
Sbjct: 246 LTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAGKPGNT 305

Query: 307 IMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTELVKKM 366
           IMVTTRN EVATMVE ISI+ LK                   LPMN+KLE +K ELV+KM
Sbjct: 306 IMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKELVRKM 365

Query: 367 GGVPLVAKVLGGAVKFEEAEFKEGDYE-TWMMKVESIARNISIEDKEFVLSILKLSVDSL 426
           GGVPLVAKVLGGAVKFEE E +E D+E +WM KVESI RNIS+EDK+FVLSILKLSVDSL
Sbjct: 366 GGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKLSVDSL 425

Query: 427 PNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERE-NLIMEDIGEQYFNFLL 486
           PNPVLKQC AYCSNFSQDYDFQKDDLI+MWIAQ FIQP Q R+ NL+MEDIGEQYFNFLL
Sbjct: 426 PNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLL 485

Query: 487 SRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLIFD 546
           SRS+FQDVTRD N RIV FKMHDL+HDIACAIS HQN+ESN PNNLS KS RKL  LI +
Sbjct: 486 SRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESN-PNNLSGKSVRKLRTLICN 545

Query: 547 DEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSLLY 606
           DEV++Y   +D+VCLRVLK+      DLWIP+DKLIHLRYLDIS+        +SLSLLY
Sbjct: 546 DEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESLSLLY 605

BLAST of PI0013145 vs. NCBI nr
Match: XP_038877687.1 (putative disease resistance protein RGA1 [Benincasa hispida] >XP_038877688.1 putative disease resistance protein RGA1 [Benincasa hispida])

HSP 1 Score: 823.2 bits (2125), Expect = 1.5e-234
Identity = 440/640 (68.75%), Postives = 504/640 (78.75%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVLKKIVK+GAEQIG+AW LE E+S L++ L  A++IL DIN+KK H  
Sbjct: 1   MAEFLWTFAVQEVLKKIVKFGAEQIGLAWGLEKEVSQLRNWLLKAETILADINKKKLHIA 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV+ WV+ L+DIV+EADDLLDELVYEHLRR VE T K R+V DSIS SKN FLFRRK AK
Sbjct: 61  SVRLWVEDLQDIVYEADDLLDELVYEHLRRAVEQTGKLREVCDSISLSKNPFLFRRKKAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           +M+NIT  LYKHYC AS  GLV  ES  ETE+ L QIRETTSILDF+V  REAE  E+LK
Sbjct: 121 QMKNITQALYKHYCEASPLGLVSDESATETEVTLAQIRETTSILDFKVRGREAEFQEILK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVI+S+NEH MSVISIVGMGGLGKTTLAKM+FNHDAIK HFD+TIWVCVSKPFIV K   
Sbjct: 181 LVIESSNEHEMSVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTIWVCVSKPFIVMKILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            KEALL RLR+ M  K+YFLVLDDVWD +  LWDELSGCLK I+GK
Sbjct: 241 AIFQGSTNTCSGLNSKEALLKRLREAMQGKRYFLVLDDVWDKDNGLWDELSGCLKQINGK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTEL 360
           SGN +MVTTR+VEVATMV T+S+HHLK                +  L MN+K+E +K  L
Sbjct: 301 SGNCVMVTTRSVEVATMVNTVSVHHLKELSDDQCWVLFKESANVNQLSMNSKIEIVKDLL 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSV 420
           V+KMGGVPLVAKVLGGAVKFEE +F++GDYE+W+ KVESIA+NI  ED++FVLSILKLSV
Sbjct: 361 VRKMGGVPLVAKVLGGAVKFEEVDFEKGDYESWITKVESIAKNILKEDRDFVLSILKLSV 420

Query: 421 DSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNF 480
           DSLP  VLKQCFAYCSNF QDYDF+KD+LIQMWIAQ FIQ QQERENL+MEDIGE+YFNF
Sbjct: 421 DSLPLLVLKQCFAYCSNFPQDYDFKKDELIQMWIAQGFIQSQQERENLVMEDIGEEYFNF 480

Query: 481 LLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLI 540
           LLSRSLFQDV RDEN RI+TFKMHDL+HDIACAIS +QNM SN PNNLS +S  KL  L+
Sbjct: 481 LLSRSLFQDVIRDENKRIITFKMHDLMHDIACAISNNQNMVSN-PNNLSGRSAGKLRTLV 540

Query: 541 FDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSL 600
           + DE   YKV D+   LRVLK ++  A +L   LDKLIHLRYLD+S        ++SL L
Sbjct: 541 YRDEENQYKVCDNFFRLRVLKQDKIWANNLPYLLDKLIHLRYLDLSKCYIIKNLRESLCL 600

Query: 601 LYNLQTLKLGFIEGDLPKNLRKLVNLRHLEFSCI-DMKQM 615
           LYNLQTLKLG+IE +LP+NLRKLVNLRHLEF  +  MKQM
Sbjct: 601 LYNLQTLKLGYIESELPENLRKLVNLRHLEFYVVPGMKQM 639

BLAST of PI0013145 vs. NCBI nr
Match: XP_038877689.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 802.0 bits (2070), Expect = 3.7e-228
Identity = 439/641 (68.49%), Postives = 491/641 (76.60%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQE+LKKIVK+GAEQIGVAW LE E   LK  L  A++IL DIN+KK H+ 
Sbjct: 1   MAEFLWTFAVQEILKKIVKFGAEQIGVAWGLEKEEFQLKKWLLKAETILADINKKKLHHH 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           +V+ WV+ L+DIV+EADDLLDEL YE LRRT E T KF+KVRDSISPSKNSFLFR KMA 
Sbjct: 61  AVRLWVEDLQDIVYEADDLLDELRYEDLRRTGEQTGKFKKVRDSISPSKNSFLFRLKMAN 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           KM+ IT  L+KHYC AS  GLVG ES  E E+AL+QIRETTSILDF+V  RE EVLE+LK
Sbjct: 121 KMKKITAALHKHYCEASPLGLVGEESATEIEVALQQIRETTSILDFEVVGREDEVLEILK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVIDS+NEH MS+ISIVGMGGLGKTTLAKM+FNHDAIK HFDQTIWVCVSKPFIV K   
Sbjct: 181 LVIDSSNEHDMSMISIVGMGGLGKTTLAKMVFNHDAIKGHFDQTIWVCVSKPFIVMKILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            KEALL RLRKEM  KKYFLVLDDVW+ E  LWDEL+G LK I+GK
Sbjct: 241 AIFQSLTNSSSGLNSKEALLQRLRKEMQSKKYFLVLDDVWEKENDLWDELNGYLKQIAGK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYPMIN-------------LPMNAKLESIKTEL 360
           SGN IMVTTRNVEVATMV+T+SIHHL+     N             LPMN+  + +K  L
Sbjct: 301 SGNSIMVTTRNVEVATMVKTVSIHHLQTLSDDNCWSLFEKCANASQLPMNSNFKIMKNML 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGD-YETWMMKVESIARNISIEDKEFVLSILKLS 420
           V+K+GGVPLVAKVLGGA+KF      EGD YE WM KVESI RNI  ED+ FVLSIL+LS
Sbjct: 361 VRKIGGVPLVAKVLGGALKF------EGDHYERWMTKVESITRNILKEDEAFVLSILRLS 420

Query: 421 VDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFN 480
           VDSLP   LKQCFAYCSNF QDY+F+KD+LIQMWIAQ FIQPQ+ERENLIMEDIGE+YFN
Sbjct: 421 VDSLPLLTLKQCFAYCSNFPQDYNFKKDELIQMWIAQGFIQPQEERENLIMEDIGEEYFN 480

Query: 481 FLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHML 540
           FLLSRSLFQDV +DEN RIVTFKMHDLIHDIAC +S HQ   SN PNN S KS R L  L
Sbjct: 481 FLLSRSLFQDVDKDENGRIVTFKMHDLIHDIACMVSNHQKKASN-PNNWSGKSARTLRAL 540

Query: 541 IFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLS 600
           I ++E   +K+  ++ CLRVL LN     +L I +DKLIHLRYLDIS        Q+SLS
Sbjct: 541 ICNNEETPHKIQANINCLRVLVLNSFETNNLSIIMDKLIHLRYLDISKCSVNQLSQESLS 600

Query: 601 LLYNLQTLKLGFIEGDLPKNLRKLVNLRHLEF-SCIDMKQM 615
            LYNLQTLK G+I  DLPKNLRKLVNLRHLEF    DMKQM
Sbjct: 601 SLYNLQTLKFGYIGSDLPKNLRKLVNLRHLEFQKKFDMKQM 634

BLAST of PI0013145 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 779.2 bits (2011), Expect = 2.6e-221
Identity = 424/640 (66.25%), Postives = 489/640 (76.41%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M EFLWTFAVQEVLKKIV +GAEQI +AW LE ELS LK  L  A +IL DIN KKSH+ 
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
           SV  WV++L DI++EADDLLDE+VYE +R+TVE T K RKVRDSISPSKNSFLF  KMAK
Sbjct: 61  SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLELLK 180
           KM+ IT TLY+HYC AS  GLVG ES  E+E AL QIRETTSILDF+VE REAEVLE+LK
Sbjct: 121 KMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEILK 180

Query: 181 LVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWN 240
           LVIDST+E H+SVISIVGMGGLGKTTLAKM+FNHDAIK HFD+T+WVCVSKPFIV K   
Sbjct: 181 LVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKILE 240

Query: 241 KSF------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGK 300
             F            +EALL RLR+EM  KKYFLVLDDVWD E  LWDEL G LK+I+GK
Sbjct: 241 AIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIAGK 300

Query: 301 SGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESIKTEL 360
           SGN IMVTTR+VEVATMV+T+ I+HLK                   L MN+KLE+ K  L
Sbjct: 301 SGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTKNIL 360

Query: 361 VKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSV 420
           V+K+GGVPL+AKVLGGAVKFE     EG  E+WM K+ES ARNISIEDK+FVLSILKLSV
Sbjct: 361 VRKIGGVPLIAKVLGGAVKFE-----EGGSESWMAKIESFARNISIEDKDFVLSILKLSV 420

Query: 421 DSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNF 480
           +SLP+  LKQCFAYCSNF QDY+F KD+ IQMWIA+ FIQP+QERENL ME+IGE+Y NF
Sbjct: 421 ESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEYLNF 480

Query: 481 LLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTRKLHMLI 540
           LLSRSLF+D  +  + RIVTFK+HDL+HDIACAIS H  M+SN P + + KSTRKL  LI
Sbjct: 481 LLSRSLFEDAIK-YDGRIVTFKIHDLMHDIACAISNHHKMDSN-PISWNGKSTRKLRTLI 540

Query: 541 FDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRPQQSLSL 600
            ++E   +K+  D++CLRVL L       L   +DKLIHLRYLDIS+ +     + S+  
Sbjct: 541 CENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLRDSICA 600

Query: 601 LYNLQTLKLGFIEGDLPKNLRKLVNLRHLEF-SCIDMKQM 615
           LYNLQTLKLG+IE DLPKNLR LVNLRHLEF    DM QM
Sbjct: 601 LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQM 633

BLAST of PI0013145 vs. NCBI nr
Match: KAE8651192.1 (hypothetical protein Csa_002145 [Cucumis sativus])

HSP 1 Score: 723.4 bits (1866), Expect = 1.7e-204
Identity = 388/517 (75.05%), Postives = 420/517 (81.24%), Query Frame = 0

Query: 118 MAKKMRNITDTLYKHYCAASAFGLVGVESVIETELALKQIRETTSILDFQVESREAEVLE 177
           MAKK++NITDTL +HYCAASAFGLVGVE+V E ELAL QIRETTSILDFQVE REAEVLE
Sbjct: 1   MAKKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLE 60

Query: 178 LLKLVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTK 237
           LLKL IDSTNEHHMSVISIVGMGGLGKTTLAKMIFNH  I+ HFD+TIWVCVSKPFIVTK
Sbjct: 61  LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK 120

Query: 238 FWNKSF-------------KEALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKH 297
              K F             KEALLGRLRKEM DK YFLVLDDVWDNEKHLWDEL GCLKH
Sbjct: 121 ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH 180

Query: 298 ISGKSGNIIMVTTRNVEVATMVETISIHHLKNYP-------------MINLPMNAKLESI 357
           I+GK GN IMVTTRN EVATMVE ISI+ LK                   LPMN+KLE +
Sbjct: 181 IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM 240

Query: 358 KTELVKKMGGVPLVAKVLGGAVKFEEAEFKEGDYE-TWMMKVESIARNISIEDKEFVLSI 417
           K ELV+KMGGVPLVAKVLGGAVKFEE E +E D+E +WM KVESI RNIS+EDK+FVLSI
Sbjct: 241 KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI 300

Query: 418 LKLSVDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERE-NLIMEDIG 477
           LKLSVDSLPNPVLKQC AYCSNFSQDYDFQKDDLI+MWIAQ FIQP Q R+ NL+MEDIG
Sbjct: 301 LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG 360

Query: 478 EQYFNFLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNMESNTPNNLSRKSTR 537
           EQYFNFLLSRS+FQDVTRD N RIV FKMHDL+HDIACAIS HQN+ESN PNNLS KS R
Sbjct: 361 EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESN-PNNLSGKSVR 420

Query: 538 KLHMLIFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFRNRP 597
           KL  LI +DEV++Y   +D+VCLRVLK+      DLWIP+DKLIHLRYLDIS+       
Sbjct: 421 KLRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLL 480

Query: 598 QQSLSLLYNLQTLKLGFIEGDLPKNLRKLVNLRHLEF 607
            +SLSLLYNLQTLKLG  +  LPKNLRKLVNLRHLEF
Sbjct: 481 LESLSLLYNLQTLKLG--QSGLPKNLRKLVNLRHLEF 514

BLAST of PI0013145 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-52
Identity = 178/656 (27.13%), Postives = 322/656 (49.09%), Query Frame = 0

Query: 32  ENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKLEDIVHEADDLLDELVYEHLRRT 91
           EN L  L   L    ++L D   K+  N  V++WV +L D+V+ A+D LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V----EHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTLYKHYCAASAFGLVGVESV 151
           +      + + R++R  +S           +  ++  +T  L +    AS   ++G++ +
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGLKEL 155

Query: 152 IETELALKQIRETTSILD-FQVESREAEVLELLKLVI-DSTNEHHMSVISIVGMGGLGKT 211
             T +  KQ   TTS++D  +V  R+ +  E+++ +I ++  ++ ++V++IVG+GG+GKT
Sbjct: 156 --TAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKT 215

Query: 212 TLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSFK------------EALLGRLRK 271
           TL+++++N   ++ +F   +W  VS+ F V K   K ++            + L  +L++
Sbjct: 216 TLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKE 275

Query: 272 EMHDK--KYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVTTRNVEVATMVETISI 331
            +      + LVLDD+W+     WD L     H +   G+ I+VTTR+  VA+++  + +
Sbjct: 276 RLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCAVHV 335

Query: 332 HHLKN--------------YPMINLPMNAKLESIKTELVKKMGGVPLVAKVLGGAVKFEE 391
           H+L+               +      +N ++  +   +V K  G+PL  K LGG ++F  
Sbjct: 336 HNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRF-- 395

Query: 392 AEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDY 451
               EG    W   + S   ++   DK  +L +L++S   LP   LK+CFAYCS F + +
Sbjct: 396 ----EGKVIEWERVLSSRIWDLP-ADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGH 455

Query: 452 DFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRSLFQDVTRDENNRIVTFK 511
            F+KD ++ +W+A+ F+Q  +  +NL  E++G +YF+ L SRSL Q            + 
Sbjct: 456 AFEKDKVVLLWMAEGFLQQTRSSKNL--EELGNEYFSELESRSLLQKTK-------TRYI 515

Query: 512 MHDLIHDIA--------------CAISCHQN------MESNTPNNLSRKSTRKLHML--- 571
           MHD I+++A              C +   +       +  N    +  ++ R++  L   
Sbjct: 516 MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTF 575

Query: 572 -----------IFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLD---KLIHLRYLDI 615
                         D+++  K+L  L  LRVL L+  +   L  P D    + H R+LD+
Sbjct: 576 LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARL--PPDFFKNISHARFLDL 635

BLAST of PI0013145 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 184.1 bits (466), Expect = 3.4e-46
Identity = 173/641 (26.99%), Postives = 296/641 (46.18%), Query Frame = 0

Query: 35  LSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKLEDIVHEADDLLDELVYEHL-RRTVE 94
           L  LK  L  A+ +L D +++  H   VK W+  ++D   +A+D+LDEL  E L RR V 
Sbjct: 36  LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

Query: 95  HTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTLYKHYCAASAFGLVGVESVIETELA 154
                  +  ++   + +   ++K+  KM  +   L  H       GL       E +  
Sbjct: 96  EAGGLGGLFQNLMAGREA--IQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWR 155

Query: 155 LKQIRETTSILDFQVESREAEVLELLKLVI--DSTNEHHMSVISIVGMGGLGKTTLAKMI 214
                    +   ++  R  + L L+ L++  D  +    +VIS+VGM G+GKTTL +++
Sbjct: 156 QASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIV 215

Query: 215 FNHDAIKRHFDQTIWVCVSKPF------------IVTKFWNKSFKEALLGRLRKEMHDKK 274
           FN   +  HF+  +W+     F            I +   N     +L  +L+K +  K+
Sbjct: 216 FNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKR 275

Query: 275 YFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVTTRNVEVATMVETISIHHLK---- 334
           + LVLDD W      W+     +     + G+ I++TTR+  V+T+ +   I+ +K    
Sbjct: 276 FLLVLDDFWSESDSEWESFQ--VAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTN 335

Query: 335 --------NYPMINL---PMNAKLESIKTELVKKMGGVPLVAKVLGGAVKFEEAEFKEGD 394
                    +   N+    +N +LE I   + ++  G+PL A+ +   ++       + +
Sbjct: 336 EECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLR------SKPN 395

Query: 395 YETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDYDFQKDDL 454
            + W     ++++N S      +L +LKLS DSLP P LK+CFA CS F + + F +++L
Sbjct: 396 PDDWY----AVSKNFSSYTNS-ILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDREEL 455

Query: 455 IQMWIA-QRFIQPQQERENLIMEDIGEQYFNFLLSRSLFQ--DVTRDENNRIVTFKMHDL 514
           + +W+A     QP+  R    +EDIG  Y   L+++S FQ  D+T      + +F MHDL
Sbjct: 456 VLLWMAIDLLYQPRSSRR---LEDIGNDYLGDLVAQSFFQRLDIT------MTSFVMHDL 515

Query: 515 IHDIACAIS---CHQNMESNTPN--------NLSR------------------------K 574
           ++D+A A+S   C +  + N P         + SR                         
Sbjct: 516 MNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFN 575

Query: 575 STRKLHMLIFDDEVMDYKVLDDLVCLRVLKLNRSRARDLWIPLDKLIHLRYLDISDSDFR 606
           S   L  L   ++V++  +L+ L  LR+L L+  +  +L   L  L  LRYLD+S +  +
Sbjct: 576 SPTSLESLQLTEKVLN-PLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIK 635

BLAST of PI0013145 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 172.6 bits (436), Expect = 1.0e-42
Identity = 175/680 (25.74%), Postives = 302/680 (44.41%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M E + +F V+++ + + +  A   G+    + ++  LK +L    S+L+D + KK+  +
Sbjct: 1   MAEAVVSFGVEKLWELLSRESARLNGI----DEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
            V+ +++ ++DIV++ADD+++  +   LR       K + ++  +       + RRK A 
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELR------GKEKGIKKQVRTLACFLVDRRKFAS 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELAL----KQIRETTS-ILDFQVESREAEV 180
            +  IT  + +      + G+  +       L+L    ++IR+T S   +  +   +  V
Sbjct: 121 DIEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSV 180

Query: 181 LELLKLVIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIV 240
            EL+  ++++ +   + V+S+ GMGG+GKTTLA+ +F+HD ++RHFD   WVCVS+ F  
Sbjct: 181 EELVDHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTR 240

Query: 241 TKFWNKSFKE--------------ALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGC 300
              W +  ++               L G L + +   +Y LVLDDVW  E   WD +   
Sbjct: 241 KDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAV 300

Query: 301 LKHISGKSGNIIMVTTRNVEVATMVETISI----------HHLKNYPMINLPMNAKL--- 360
             H   K G  +++T+RN  +    +                 K +  I      K    
Sbjct: 301 FPH---KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEFK 360

Query: 361 --ESIKTELVKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNI------S 420
             E++  E+V   GG+PL  KVLGG +       K+     W     +I  +I      S
Sbjct: 361 VDEAMGKEMVTYCGGLPLAVKVLGGLLA------KKHTVLEWKRVHSNIVTHIVGKSGLS 420

Query: 421 IEDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQER 480
            ++   V  +L LS + LP   LK CF Y ++F +DY      L   W+A+  I P  + 
Sbjct: 421 DDNSNSVYRVLSLSYEDLPMQ-LKHCFFYLAHFPEDYKIDVKILFNYWVAEGIITPFHDG 480

Query: 481 ENLIMEDIGEQYFNFLLSRSLFQDVTRDENNRIVTFKMHDLIHDIACAISCHQNM----- 540
             +  +D GE Y   L+ R++         +RI   +MHD++ ++  + +  +N      
Sbjct: 481 STI--QDTGESYLEELVRRNMVVVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVK 540

Query: 541 ----ESNTPNNLSRKSTRK--------LHMLIFDDEVMDYKVL--------------DDL 600
                S T N  S   +R+        LHML   D      VL                L
Sbjct: 541 VPTTTSTTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCL 600

Query: 601 VCLRVLKLNRSRARDLWIP--LDKLIHLRYLDISDSDFRNRPQQSLSLLYNLQTLKLGFI 605
             LRVL L+  +     +P  +  LIHLR+L + ++   + P  SL  L  L  L LG  
Sbjct: 601 PLLRVLDLSYVQFEGGKLPSSIGDLIHLRFLSLYEAGVSHLP-SSLGNLKLLLCLNLGVA 652

BLAST of PI0013145 vs. TAIR 10
Match: AT1G50180.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-41
Identity = 177/686 (25.80%), Postives = 307/686 (44.75%), Query Frame = 0

Query: 1   MVEFLWTFAVQEVLKKIVKYGAEQIGVAWELENELSLLKDKLHDADSILEDINRKKSHND 60
           M E + +  VQ++ + +++      G+     +++  L+D+L   +  L+D + K+  ++
Sbjct: 1   MAEAIVSVTVQKLGQLLLEEPLFLFGIG----DQVKQLQDELKRLNCFLKDADEKQHESE 60

Query: 61  SVKRWVQKLEDIVHEADDLLDELVYEHLRRTVEHTEKFRKVRDSISPSKNSFLFRRKMAK 120
            V+ WV  + +  ++A+D+L+      L+      +  ++V   ++   N  +    +  
Sbjct: 61  RVRNWVAGIREASYDAEDILEAF---FLKAESRKQKGMKRVLRRLACILNEAVSLHSVGS 120

Query: 121 KMRNITDTLYKHYCAASAFGLVGVESVIETELALK-QIRETTSILDFQVESREAEVLELL 180
           ++R IT  L K   +   FG+   ES+    L+L   +RE      + VE     + + L
Sbjct: 121 EIREITSRLSKIAASMLDFGI--KESMGREGLSLSDSLREQRQSFPYVVEHNLVGLEQSL 180

Query: 181 KLVIDS--TNEHHMSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTK 240
           + +++   +    + V SI GMGGLGKTTLAK IF+H  ++RHFD+  WV VS+      
Sbjct: 181 EKLVNDLVSGGEKLRVTSICGMGGLGKTTLAKQIFHHHKVRRHFDRFAWVYVSQDCRRRH 240

Query: 241 FW-----NKSFK-----------EALLGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGC 300
            W     N S+K           E L   L + +   K  +VLDD+W   K  WD    C
Sbjct: 241 VWQDIFLNLSYKDENQRILSLRDEQLGEELHRFLKRNKCLIVLDDIWG--KDAWD----C 300

Query: 301 LKHI-SGKSGNIIMVTTRNVEVATMVETISIHH------------------LKNYPMINL 360
           LKH+   ++G+ I++TTRN EVA   +   + H                  L     I  
Sbjct: 301 LKHVFPHETGSEIILTTRNKEVALYADPRGVLHEPQLLTCEESWELLEKISLSGRENIEP 360

Query: 361 PMNAKLESIKTELVKKMGGVPLVAKVLGGAVKFEEAEFKEGDYETWMMKVESIARNISI- 420
            +  K+E I  ++V + GG+PL   VLGG +        +  +  W    E+I   +S  
Sbjct: 361 MLVKKMEEIGKQIVVRCGGLPLAITVLGGLLA------TKSTWNEWQRVCENIKSYVSNG 420

Query: 421 -----EDKEFVLSILKLSVDSLPNPVLKQCFAYCSNFSQDYDFQKDDLIQMWIAQRFIQP 480
                     V  +L LS + LP P +KQCF Y +++ +DY+     L+   IA+  + P
Sbjct: 421 GSSNGSKNMLVADVLCLSYEYLP-PHVKQCFLYFAHYPEDYEVHVGTLVSYCIAEGMVMP 480

Query: 481 QQERE-NLIMEDIGEQYFNFLLSRSLFQDVTRD-ENNRIVTFKMHDLIHDIAC------- 540
            +  E    +ED+G+ Y   L+ RS+     RD   + ++T +MHDL+ ++         
Sbjct: 481 VKHTEAGTTVEDVGQDYLEELVKRSMVMVGRRDIVTSEVMTCRMHDLMREVCLQKAKQES 540

Query: 541 ---AISCHQNMESNTPNNLSRKSTRKLHMLIFDD-EVMDYKVLDD-----LVCLRVLKLN 600
               I      E+    +LS  ++R++ + +    E    K L       +  LRVL L 
Sbjct: 541 FVQVIDSRDQDEAEAFISLSTNTSRRISVQLHGGAEEHHIKSLSQVSFRKMKLLRVLDLE 600

Query: 601 RSRARDLWIPLD--KLIHLRYLDISDSDFRNRPQQSLSLLYNLQTLKLGFIEGDL----- 608
            ++     +P D   LIHLR L +  ++ +     S+  L  + TL L F++G L     
Sbjct: 601 GAQIEGGKLPDDVGDLIHLRNLSVRLTNVKEL-TSSIGNLKLMITLDL-FVKGQLYIPNQ 660

BLAST of PI0013145 vs. TAIR 10
Match: AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 163.3 bits (412), Expect = 6.2e-40
Identity = 166/656 (25.30%), Postives = 297/656 (45.27%), Query Frame = 0

Query: 31  LENELSLLKDKLHDADSILEDINRKKSHNDSVKRWVQKLEDIVHEADDLLDELVYEHLRR 90
           +E++++ LK  L+   S L+D + KK  +  VK  V+++++I+++ +D ++  V E    
Sbjct: 27  VEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIETFVLE---- 86

Query: 91  TVEHTEKFRKVRDSISPSKNSFLFRRKMAKKMRNITDTLYKHYCAASAFGLVGVESVIET 150
             ++  K   ++ SI         RR+ A  +  +++ + K      +FG+   +++++ 
Sbjct: 87  --QNLGKTSGIKKSIRRLACIIPDRRRYALGIGGLSNRISKVIRDMQSFGV--QQAIVDG 146

Query: 151 ELALKQIRETTSIL-------DFQVESREAEVLELLKLVIDSTNEHHMSVISIVGMGGLG 210
                Q  +   +        D      EA V +L+  ++D  N   + V+SI GMGGLG
Sbjct: 147 GYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVDEAN---VQVVSITGMGGLG 206

Query: 211 KTTLAKMIFNHDAIKRHFDQTIWVCVSKPFIVTKFWNKSFK----------------EAL 270
           KTTLAK +FNH+ +K  FD   WVCVS+ F     W K  +                + L
Sbjct: 207 KTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKIMEMTQDTL 266

Query: 271 LGRLRKEMHDKKYFLVLDDVWDNEKHLWDELSGCLKHISGKSGNIIMVTTRNVEVATMVE 330
            G L + +   K  +VLDD+W  EK  W+ +        G     +++T+RN  VA    
Sbjct: 267 QGELIRLLETSKSLIVLDDIW--EKEDWELIKPIFPPTKGWK---VLLTSRNESVAMRRN 326

Query: 331 TISIHHLKN----------YPMINLPM----NAKLESIKTEL----VKKMGGVPLVAKVL 390
           T  I+              +  I LPM      K++  K EL    +K  GG+PL  +VL
Sbjct: 327 TSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLPLAIRVL 386

Query: 391 GG--AVKFEEAEFKEGDYETWMMKVESIARNISIEDKEFVLSILKLSVDSLPNPVLKQCF 450
           GG  A K+   +++    E     +     N + ++      +L LS + LP+  LK CF
Sbjct: 387 GGMLAEKYTSHDWRRLS-ENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPS-YLKHCF 446

Query: 451 AYCSNFSQDYDFQKDDLIQMWIAQRFIQPQQERENLIMEDIGEQYFNFLLSRSLFQDVTR 510
            Y ++F  DY+    +L   W A+   QP +  +  I+ D+G+ Y   L+ R++      
Sbjct: 447 LYLAHFPDDYEINVKNLSYYWAAEGIFQP-RHYDGEIIRDVGDVYIEELVRRNMVISERD 506

Query: 511 DENNRIVTFKMHDLIHDIACAISCHQNM------ESNTPNNLSRKSTRKL-----HMLIF 570
            + +R  T  +HD++ ++    +  +N        ++T N+LS  ++R+L       L  
Sbjct: 507 VKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPITLDV 566

Query: 571 DDEVMDYKVLDDLV------------------------CLRVLKLNRSRAR--DLWIPLD 605
           + ++ D K+   +V                         LRVL ++R++ +   L   + 
Sbjct: 567 EKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLELLRVLDIHRAKLKGGKLASSIG 626

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XA422.1e-6932.54Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ93.6e-6932.17Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA401.5e-6732.29Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA393.4e-6732.81Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR41.5e-5127.13Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0LBV05.1e-26077.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1[more]
A0A0A0KST51.2e-22166.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1[more]
A0A6J1CVR68.4e-19460.22putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CU861.9e-18558.32disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1CUL86.4e-17855.63putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004136783.21.1e-25977.42disease resistance protein RGA2 [Cucumis sativus][more]
XP_038877687.11.5e-23468.75putative disease resistance protein RGA1 [Benincasa hispida] >XP_038877688.1 put... [more]
XP_038877689.13.7e-22868.49putative disease resistance protein RGA3 [Benincasa hispida][more]
XP_004140442.12.6e-22166.25disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical prote... [more]
KAE8651192.11.7e-20475.05hypothetical protein Csa_002145 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G14470.11.1e-5227.13NB-ARC domain-containing disease resistance protein [more]
AT3G14460.13.4e-4626.99LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G53350.11.0e-4225.74Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G50180.11.9e-4125.80NB-ARC domain-containing disease resistance protein [more]
AT1G58848.16.2e-4025.30Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 62..82
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 550..566
score: 26.62
coord: 339..353
score: 36.5
coord: 254..268
score: 43.17
coord: 193..208
score: 56.41
NoneNo IPR availableGENE3D1.20.5.4130coord: 8..126
e-value: 1.9E-14
score: 55.7
NoneNo IPR availablePANTHERPTHR23155:SF1139NBS-LRR TYPE DISEASE RESISTANCE PROTEINcoord: 6..606
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 477..608
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 155..311
e-value: 1.6E-30
score: 107.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 168..410
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 185..396
e-value: 3.8E-30
score: 104.9
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 9..97
e-value: 6.3E-16
score: 58.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 390..488
e-value: 5.7E-15
score: 57.2
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 493..617
e-value: 2.8E-10
score: 41.0
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 6..606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013145.1PI0013145.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding