PI0013011 (gene) Melon (PI 482460) v1

Overview
NamePI0013011
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description(R)-mandelonitrile lyase 1-like
Locationchr05: 5049340 .. 5052652 (-)
RNA-Seq ExpressionPI0013011
SyntenyPI0013011
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTATCCCAATAACATTTAGCTTTTGAAAGGCCATGGGACATTCTAGGGTTACCAATCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACGACTTCACTCCTCACATGCTATCCCCAATCAAGGTACAAATTATTGTAGAATAAGTCATCGTCACAACTCGGATGACAGTTTTACAACGTTCGTTACGTGTCACCTTTATTTTATTTAAAATTTTCCTTCATTGATACTTATTTTGGTATGAACTTTAATTTTCCAGATTTTAGCTACATGAAGTTTGTACATAATGCCACTGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAAAAATCCCTTTCAACGTTTCGTCTCGGAGGACGGCGTAGAGAACATAAGAGGACGAGTCCTTGGCGGGGGAAGCATGGTCAATGCTGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATACTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGCTGGTGTTGTTCCTGATAATGGGTTTGATGTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTTTTCTCTGGTTAGTCCTTTGCAAATATTCAATTTAAATTATATTTGTTTTAATTTACTAGACTTCTAATTTATACTTTAACGTAATCTCCTAACGAAAATTGAGCCAATGACTTTATAATGTTTCACTAAGATAATTCTAATGCTAGTGTTTAATGAGTAATTGTTTTCCTAGGATTGAACAAATTACTTTTCTTGTGGAAGCAACACTCTTTCACAAGATCCAATAAACTATACTTGGTGAAAATAAATTGAACTTGAGGAGTTATTCCAATAGACTATCCGTAGTGCTTGGGAAAAGTTATTTTAAAATGCATTAATTTTGAAGAAAGACATATTTCGAGTTCATATCTCTTCTTCGAATTAATTAATATAACTTCACACGAATTGTCACATATCATTTATTCCAATGGATAATTTCCATAATTTTTGCTCATGTATTATTTTGACTCGTAGGTTTATCAGCAAATGGGGTTTTGTATTCAGATTCAAAAGGAAAGATACACACAGCATTCATTCGCAAAAAAGGAGAAATCATTCTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCATCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAAGTACCTGTCGTTCTTCACCAACCATATGTCGGTCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCCTTCCATTCCCAGTCCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAGTCTATTGCCAGCCCTTTACCAATTTTAATTCCCCCAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATATTTGTTGGAAAATTCTCGGAGGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCCACTAATGTGAAAAAGAGTCCCATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCAAACTATGGAAAAGATTAAGACGCAAAATTTGGAGGGGAATAAAGGATTTGAGTTTTTGGGGGTTCCATTGCCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAAACGGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTCGAAAAAGTGGTTGACGGTAATTATAAAGTCATCGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGGTGAGTATTTTCTTTTTCGTTCTTTCCTTTTTTTTTTTTTTTTGTTAGTACAATAATTAGGATAAGCTAAGCCATTGTGGTCAAGTAATTCTTTTATAAATGTGTCTAAAATAAACGTTTTCACGTATAACACAATAAACTAAAATATTTACAAAATATAACCAAATTTAAGATTTTAGTATTGATAAATATTTGACAGACATCTATTAATATCTATCGAGTATATTATTAATAGAATCTGAAATTTTACTATATTTTTATAAATATTTTCAACAGTTTTGTCATTTACGATAATTTATTTAAATTTTATTTGCTTTGAGTTATTATGTAGTTTCAAATTTTTTTATAACTAAAAATCAACCATTATTTTTAGTTTTTAAAACTTGTTTGGTTTTTAAAACACTACTAGACAAAGTAATTTAAAAAAATCTTATTAGTTTAATTTTAAAAAAATTAAAAAGGAAACTAAATAATTCTTTAACATTACCTTAGAGACCTATAAAAAAAAATAAACTTAGACCTATATATTATTCACAATCCTACAAAAGGACTAATTATAAGCTTATAATTTAATTAACTCCAAAAAAAAAAAAAAGAAAAATCCAATTCGATGTCATTGACTCTTAAATTGATTTTTTTAATCCTCAAACCCCATTTAGGACTTAGAAATAATTTTGAAAATTAAAGTAAAAGGTATTATCAAAACCTAGGAGTTTTATTTTACAAAATAACAACATATAATATTTTCTTATAAAGAAATAGGCTCATATGTTAAGACATATGCTGTACTTTGTGATTTTATTTCAATATTATTCTCCATCATTTCTATGAACAAATTTCAAAATAATTTTGCAGATATGTTGGCCTTAAGGTACTCCAACAAAGATCGAGTGTCAACTAATTAAGAAAGGCCAATTTTTGTTCAATATTCTTCTTCGTTGTCTGCATGTTTCTTGTACTTTCTTCTTTAATTATATAAGTGTGTTTTATGATAATTATTGTTATTTATTTTTCGTTTTTCATATTTTTAATTAGTTCCTACAAATTGTATTTTTTTCTTCTTATTAATTCCCACTTTTTACTACATGAATAAACTTATTAGCTTGATTC

mRNA sequence

TTGTATCCCAATAACATTTAGCTTTTGAAAGGCCATGGGACATTCTAGGGTTACCAATCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACGACTTCACTCCTCACATGCTATCCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCACTGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAAAAATCCCTTTCAACGTTTCGTCTCGGAGGACGGCGTAGAGAACATAAGAGGACGAGTCCTTGGCGGGGGAAGCATGGTCAATGCTGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATACTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGCTGGTGTTGTTCCTGATAATGGGTTTGATGTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTTTTCTCTGGTTTATCAGCAAATGGGGTTTTGTATTCAGATTCAAAAGGAAAGATACACACAGCATTCATTCGCAAAAAAGGAGAAATCATTCTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCATCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAAGTACCTGTCGTTCTTCACCAACCATATGTCGGTCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCCTTCCATTCCCAGTCCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAGTCTATTGCCAGCCCTTTACCAATTTTAATTCCCCCAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATATTTGTTGGAAAATTCTCGGAGGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCCACTAATGTGAAAAAGAGTCCCATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCAAACTATGGAAAAGATTAAGACGCAAAATTTGGAGGGGAATAAAGGATTTGAGTTTTTGGGGGTTCCATTGCCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAAACGGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTCGAAAAAGTGGTTGACGGTAATTATAAAGTCATCGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGATATGTTGGCCTTAAGGTACTCCAACAAAGATCGAGTGTCAACTAATTAAGAAAGGCCAATTTTTGTTCAATATTCTTCTTCGTTGTCTGCATGTTTCTTGTACTTTCTTCTTTAATTATATAAGTGTGTTTTATGATAATTATTGTTATTTATTTTTCGTTTTTCATATTTTTAATTAGTTCCTACAAATTGTATTTTTTTCTTCTTATTAATTCCCACTTTTTACTACATGAATAAACTTATTAGCTTGATTC

Coding sequence (CDS)

ATGGGACATTCTAGGGTTACCAATCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACGACTTCACTCCTCACATGCTATCCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCACTGATTTACCAACAAAAGAAGAATATGACTACATAATTATAGGAGGAGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAAAAATCCCTTTCAACGTTTCGTCTCGGAGGACGGCGTAGAGAACATAAGAGGACGAGTCCTTGGCGGGGGAAGCATGGTCAATGCTGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATACTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGCTGGTGTTGTTCCTGATAATGGGTTTGATGTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTTTTCTCTGGTTTATCAGCAAATGGGGTTTTGTATTCAGATTCAAAAGGAAAGATACACACAGCATTCATTCGCAAAAAAGGAGAAATCATTCTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCATCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAAGTACCTGTCGTTCTTCACCAACCATATGTCGGTCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCCTTCCATTCCCAGTCCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAGTCTATTGCCAGCCCTTTACCAATTTTAATTCCCCCAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATATTTGTTGGAAAATTCTCGGAGGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCCACTAATGTGAAAAAGAGTCCCATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCAAACTATGGAAAAGATTAAGACGCAAAATTTGGAGGGGAATAAAGGATTTGAGTTTTTGGGGGTTCCATTGCCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAAACGGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTCGAAAAAGTGGTTGACGGTAATTATAAAGTCATCGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCTATGGCCACTCTCATGATGCTTGGCCGATATGTTGGCCTTAAGGTACTCCAACAAAGATCGAGTGTCAACTAA

Protein sequence

MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILIPPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQRSSVN
Homology
BLAST of PI0013011 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.1e-161
Identity = 281/539 (52.13%), Postives = 380/539 (70.50%), Query Frame = 0

Query: 9   LILSLMISIFQL-RLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLAT 68
           L+L L + + Q   +HS     N DFSY++F ++ATDL  +  YDY+I+GGGT+GCPLA 
Sbjct: 11  LVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAA 70

Query: 69  TLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVENIRGRVL 128
           TLS K+ VL+LERGS P  YP+VL   G +      DDGK P +RFVSEDG++N+RGRVL
Sbjct: 71  TLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVL 130

Query: 129 GGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRSSLL 188
           GG SM+NAG Y+R +   ++++GVDWDM+LV K YEWVE+T+V +P    WQS   ++ L
Sbjct: 131 GGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFL 190

Query: 189 EAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFS 248
           EAGV P++GF + H  GT+  GS FDNKG RH A ELLNK N  N++V + A+V++I+FS
Sbjct: 191 EAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFS 250

Query: 249 ---GLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSSLKVP 308
              GL+A GV+Y DS G  H AF+R KGE+I+SAG IG+PQLL LSG+GP+S+LSSL +P
Sbjct: 251 NAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIP 310

Query: 309 VVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILIPPIF 368
           VVL  PYVGQ + DNPR   NI+ P P+ P+ V V+GI  D  + Q   S LP   PP F
Sbjct: 311 VVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP-F 370

Query: 369 SLLPPQATSI-IPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCV 428
           S  P  +  +   T A F  K +   S GSL L SS+NV+ SP V+FNYYS+P DL  CV
Sbjct: 371 SFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCV 430

Query: 429 KGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHY 488
            G++K+G+LL T  ++  K ++L G +GF  LG+PLP++  +D++ E +C+++V +YWHY
Sbjct: 431 SGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHY 490

Query: 489 HGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQRSS 543
           HGGCLV KV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+LQ+RS+
Sbjct: 491 HGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of PI0013011 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.3e-159
Identity = 280/550 (50.91%), Postives = 386/550 (70.18%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLR---LHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIG 60
           M  S ++ ++L L I +  L+   +HS     + DFSY+ F ++ATDL  +  YDY+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSED 120
           GGT+GCPLA TLS K+ VL+LERGS P  YP+VL   G +      DDGK P +RFVSED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNT 180
           G++N+RGRVLGG S++NAG Y+R +   ++++GVDWDM+LV + YEWVE+T+V +P   +
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 181 WQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQS  +++ LEAGV P++GF + H  GT+  GS FDNKG RH A ELLNK N  N++V +
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 EATVQRILFS---GLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGP 300
            A+V++I+FS   GL+A GV+Y DS G  H AF+R KGE+I+SAG IG+PQLL LSG+GP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIAS 360
           +S+LSSL +PVVL  PYVGQ + DNPR   NI+ P P+ P+ V V+GI  D  + Q   S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFS 360

Query: 361 PLPILIPPIFSLLPPQATSIIP--TLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNY 420
            LP   PP F    P A+  +P  T A F  K +   S GSL L SS+NV+ SP V+FNY
Sbjct: 361 SLPFTTPP-FGFF-PSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+G+LL T  ++  K ++L G +GF  LG+PLP++  +D++ E +
Sbjct: 421 YSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETF 480

Query: 481 CKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C+++V +YWHYHGGCLV KV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGRYV
Sbjct: 481 CRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYV 540

Query: 541 GLKVLQQRSS 543
           G+K+LQ+RS+
Sbjct: 541 GIKILQERSA 546

BLAST of PI0013011 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 1.0e-155
Identity = 274/550 (49.82%), Postives = 380/550 (69.09%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPN---QDFSYMKFVHNATDLPTKEEYDYIIIG 60
           M  S ++ ++L L I +  L+     ++ N    DFSY+ FV++ATD   +  YDYII+G
Sbjct: 1   MVKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSED 120
           GGTAGCPLA TLS+ +SVL+LERGS P +YP++L   G +      DDGK P +RFVSED
Sbjct: 61  GGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNT 180
           G++N+RGRVLGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V +P   T
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQT 180

Query: 181 WQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQ+   ++ LEAG++P+NGF V HL GT+  GS FDN G RH + ELLNK +P N++VA+
Sbjct: 181 WQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAV 240

Query: 241 EATVQRILF----SGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIG 300
           +A V++I+F    SG++A GV+Y+DS G  H AF+R +GE+ILSAG IGSPQLL LSG+G
Sbjct: 241 QAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVG 300

Query: 301 PKSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIA 360
           P+S+L+SL + VV   PYVGQ + DNPR   NI+ P P+  S+V V+GI  D  + Q   
Sbjct: 301 PESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSI 360

Query: 361 SPLPILIPPIFSLLPPQATSI-IPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNY 420
           S LP   PP FS  P  +  +   T A  V K     S G++ LNSS++V+  P V+FNY
Sbjct: 361 SSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+G++L T  +E  K ++L G  GF  LG+PLPEN  +D++ E +
Sbjct: 421 YSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETF 480

Query: 481 CKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C+++V +YWHYHGGCLV KV+D  ++V GI  LRVVDGSTF  +P ++P    +MLGRY+
Sbjct: 481 CRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYM 540

Query: 541 GLKVLQQRSS 543
           G+++LQ+RS+
Sbjct: 541 GIQILQERSA 547

BLAST of PI0013011 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 273/551 (49.55%), Postives = 373/551 (67.70%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPN----QDFSYMKFVHNATDLPTKEEYDYIII 60
           M  S ++ ++L L + +  L+    H++ N     DF Y+KFV+NA DL  +  YDYII+
Sbjct: 1   MEKSTMSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIV 60

Query: 61  GGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSE 120
           GGGT+GCPLA TLS+ +SVL+LERG+   +YP+ L   G        DDGK P +RFVSE
Sbjct: 61  GGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSE 120

Query: 121 DGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILN 180
           DG++N+R R+LGG +++NAG Y+R +  F+ ++GV+WD++LV +AYEWVE+ +V +P   
Sbjct: 121 DGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQ 180

Query: 181 TWQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA 240
           +WQS   ++ LEAGV PDNGF + H  GT+  GS FDN G RH + ELLNK +P N+KVA
Sbjct: 181 SWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVA 240

Query: 241 IEATVQRILF----SGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGI 300
           +EA VQ+I+F    SGL+A GV+Y+DS G  H A +  KGE+ILSAG +G+PQLL LSG+
Sbjct: 241 VEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGV 300

Query: 301 GPKSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSI 360
           GP+S+L+SL + VV   PYVGQ ++DNPR   NI+ P P+ PS+V V+GI  D  + Q  
Sbjct: 301 GPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCS 360

Query: 361 ASPLPILIPPIFSLLPPQATSI-IPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFN 420
            S LP   PP FSL P  +  +   T A  V K     S GSL L SS+NV  +P V+FN
Sbjct: 361 LSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFN 420

Query: 421 YYSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEE 480
           Y S P DL  CV G++K+G  L T  ++  K  +L G  GF  LG PLPEN  +D++ E+
Sbjct: 421 YCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEK 480

Query: 481 YCKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRY 540
           +C+ TV +YWHYHGG +V KV+DGN++V GI  LRVVDGSTF  +P ++P    +MLGRY
Sbjct: 481 FCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGRY 540

Query: 541 VGLKVLQQRSS 543
           VG K++Q+RS+
Sbjct: 541 VGTKIVQERSA 548

BLAST of PI0013011 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.9e-151
Identity = 271/549 (49.36%), Postives = 370/549 (67.40%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPN---QDFSYMKFVHNATDLPTKEEYDYIIIG 60
           M  S ++ ++  L + +  L+    H++ N    DFSY+KFV+NATD   +  YDYI+IG
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSED 120
           GGT+GCPLA TLS K+ VLLLERG+   +YP+ L   G        DDGK P +RFVSED
Sbjct: 61  GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNT 180
           G++N+R R+LGG +++NAG Y+R +  F++  G++WD++LV K YEWVE+ +V +P   +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQS 180

Query: 181 WQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQS      LEAG++PDNGF + H  GT+  GS FDN G RH A ELLNK +P N+ VA+
Sbjct: 181 WQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAV 240

Query: 241 EATVQRILF----SGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIG 300
           +A+V++ILF    S LSA GV+Y+DS G  H AF+R  GE+I+SAG IG+PQLL LSG+G
Sbjct: 241 QASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVG 300

Query: 301 PKSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIA 360
           P+S+LSSL + VV   PYVGQ + +NPR   N   P P+  S V V+GI  D  Y Q   
Sbjct: 301 PESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSL 360

Query: 361 SPLPILIPPIFSLLPPQATSI-IPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNY 420
           S LP   PP FSL P  +  +   T A  V +     S GS+ LNSS++V+ +P ++FNY
Sbjct: 361 SSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+GDLL+T+ +E  K +++ G  GF +LGVPLPEN  +D+S E +
Sbjct: 421 YSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETF 480

Query: 481 CKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C   V +YWHYHGG LV KV+D +++V+GIK LRVVD STF   P ++P    +MLGRYV
Sbjct: 481 CLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYV 540

Query: 541 GLKVLQQRS 542
           GL++LQ+RS
Sbjct: 541 GLQILQERS 546

BLAST of PI0013011 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.0e-293
Identity = 505/530 (95.28%), Postives = 517/530 (97.55%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M HSRVT LILSLMISIFQL+LHSSHAIPNQD SYMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GVVPD+GFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H A IRKKGEII+SAGAIGSPQLL LSGIGPKSHLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASP PILI
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           P IFSLLPPQATSIIPTLA+FVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDLG
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGCLV KVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of PI0013011 vs. ExPASy TrEMBL
Match: A0A1S3BSB5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 3.0e-288
Identity = 499/544 (91.73%), Postives = 520/544 (95.59%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M H RVT LILSLMISIFQL+LHSS AIPNQD  YMKFVHNATDLPTKEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GV+PDNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H AFIR+KGEIILSAGAIGSPQLL LSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PPIFS L  Q TSI PT AIFVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDLG
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKTQNLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540
           WHYHGGC+V KVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLKVLQ+R
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQER 540

Query: 541 SSVN 545
            ++N
Sbjct: 541 LNLN 543

BLAST of PI0013011 vs. ExPASy TrEMBL
Match: A0A5A7VNU1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001020 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.2e-282
Identity = 489/530 (92.26%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M H RVT LILSLMISIFQL+LHSS AIPNQD  YMKFVHNATDLPTKEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GV+PDNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H AFIR+KGEIILSAGAIGSPQLL LSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PPIFS L  Q TSI PT AIFVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDLG
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKTQNLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+V KVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of PI0013011 vs. ExPASy TrEMBL
Match: A0A1S3BS35 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493093 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 479/531 (90.21%), Postives = 502/531 (94.54%), Query Frame = 0

Query: 14  MISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKF 73
           MISIFQL+LHSSHAIPNQD SYMKFVHNATDL TKEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVENIRGRVLGGGSMV 133
           SVL+LERGSDPNKYP VLNE GL NVF AGDDGKNP QRF+SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVENIRGRVLGGGSMV 120

Query: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRSSLLEAGVVP 193
           NAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILN WQS FRSSLLE+GVVP
Sbjct: 121 NAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQSTFRSSLLESGVVP 180

Query: 194 DNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253
           DNGFD+RHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEATVQRILFSGLSANG 240

Query: 254 VLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSSLKVPVVLHQPYVG 313
           VLYSDSKGK+H AFIRKKGEIILSAGAIGSPQLL LSGIGPKS+LSSLK+P+VLHQPYVG
Sbjct: 241 VLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPLVLHQPYVG 300

Query: 314 QSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILIPPIFSLLPPQATS 373
           QSMSDNPRFGT  ILPFPVLPSS K VGILQDNIYIQSIAS LPILIPPIFS LPP ATS
Sbjct: 301 QSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILIPPIFSFLPPGATS 360

Query: 374 IIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCVKGVRKMGDLL 433
           I P+L + +GKFSEVHSEGSLRLNSST+VK+SPIVRFNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 361 IDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 420

Query: 434 KTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVEKVV 493
           KT+TMEKIKTQNLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TYWHYHGGC+V KVV
Sbjct: 421 KTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTYWHYHGGCVVGKVV 480

Query: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQRSSVN 545
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLK+LQQRS++N
Sbjct: 481 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKLLQQRSNLN 531

BLAST of PI0013011 vs. ExPASy TrEMBL
Match: A0A5A7VGR6 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001190 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.1e-277
Identity = 479/530 (90.38%), Postives = 499/530 (94.15%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M HSRVT LILSLMISIFQL+LHSSHAIPNQD SYMKFVHNATDL TKEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVL+LERGSDPNKYP VLNE GL NVF AGDDGKNP QRF+SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
            FRSSLLE+GVVPDNGFD+RHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEAT
Sbjct: 181 TFRSSLLESGVVPDNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H AFIRKKGEIILSAGAIGSPQLL LSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+P+VLHQPYVGQSMSDNPRFGT  ILPFPVLPSS K VGILQDNIYIQSIAS LPILI
Sbjct: 301 LKLPLVLHQPYVGQSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILI 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PPIFS LPP ATSI P+L + +GKFSEVHSEGSLRLNSST+VK+SPIVRFNYYSHPDDLG
Sbjct: 361 PPIFSFLPPGATSIDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKTQNLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+V KVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 530

BLAST of PI0013011 vs. NCBI nr
Match: XP_011650116.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.7e-298
Identity = 515/543 (94.84%), Postives = 529/543 (97.42%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M HSRVT LILSLMISIFQL+LHSSHAIPNQD SYMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GVVPDNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H A IRKKGEII+SAGAIGSPQLL LSGIGPKSHLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASP PILI
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           P IFSLLPPQATSIIPTLA+FVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDLG
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540
           WHYHGGCLV KVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLK+L QR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540

Query: 541 SSV 544
           S++
Sbjct: 541 SNL 543

BLAST of PI0013011 vs. NCBI nr
Match: KAE8651660.1 (hypothetical protein Csa_021193 [Cucumis sativus])

HSP 1 Score: 1018.1 bits (2631), Expect = 2.9e-293
Identity = 504/530 (95.09%), Postives = 518/530 (97.74%), Query Frame = 0

Query: 14  MISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKF 73
           MISIFQL+LHSSHAIPNQD SYMKFVHNATDLP KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVENIRGRVLGGGSMV 133
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120

Query: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRSSLLEAGVVP 193
           NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILN WQSAFRSSLLE GVVP
Sbjct: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180

Query: 194 DNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253
           DNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240

Query: 254 VLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSSLKVPVVLHQPYVG 313
           VLYSDSKGK+H A IRKKGEII+SAGAIGSPQLL LSGIGPKSHLSSLK+PVVLHQPYVG
Sbjct: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300

Query: 314 QSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILIPPIFSLLPPQATS 373
           QSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASP PILIP IFSLLPPQATS
Sbjct: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 360

Query: 374 IIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCVKGVRKMGDLL 433
           IIPTLA+FVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDLGRCVKGVRKMGDLL
Sbjct: 361 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420

Query: 434 KTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVEKVV 493
           KT+TMEKIKT+NLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLV KVV
Sbjct: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480

Query: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQRSSV 544
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLK+L QRS++
Sbjct: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 530

BLAST of PI0013011 vs. NCBI nr
Match: XP_008452041.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 1000.3 bits (2585), Expect = 6.2e-288
Identity = 499/544 (91.73%), Postives = 520/544 (95.59%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M H RVT LILSLMISIFQL+LHSS AIPNQD  YMKFVHNATDLPTKEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GV+PDNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H AFIR+KGEIILSAGAIGSPQLL LSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PPIFS L  Q TSI PT AIFVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDLG
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKTQNLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540
           WHYHGGC+V KVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLKVLQ+R
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQER 540

Query: 541 SSVN 545
            ++N
Sbjct: 541 LNLN 543

BLAST of PI0013011 vs. NCBI nr
Match: KAA0067281.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 979.9 bits (2532), Expect = 8.7e-282
Identity = 489/530 (92.26%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M H RVT LILSLMISIFQL+LHSS AIPNQD  YMKFVHNATDLPTKEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILN WQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLE GV+PDNGFD+RHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGK+H AFIR+KGEIILSAGAIGSPQLL LSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PPIFS L  Q TSI PT AIFVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDLG
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKTQNLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+V KVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of PI0013011 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 968.8 bits (2503), Expect = 2.0e-278
Identity = 478/544 (87.87%), Postives = 507/544 (93.20%), Query Frame = 0

Query: 1   MGHSRVTNLILSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGT 60
           M HSR T LILSLMISIF L L SSHAIPNQD  YMKFVH+ATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVE 120
           AGCPL++TLSSKFSVLLLERGSDPNKYPSVLNEQGLLN FAA DDGKNPFQ F+SEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQS 180
           NIRGRVLGGGSMVN GFYSRGH++FF +AGVDWDMELVEKAY+WVEETVVSQPILN WQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRS+ L+AGVVPDNGFD+RH VGTK GGSIFDNKGNRHGAVELLNKANPTNIKV IEAT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKSHLSS 300
           VQRILFSGLSA GVLYSDSKGK+H AFIRK+GEII+SAGA+GSPQLL LSG+GPKSHLSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPLPILI 360
           LK+PVVLHQPYVG+ MSDNPRFGTNIILPF ++PSSVKVVGILQDNIYIQS ASP P   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLG 420
           PP FSLLPPQ  SI+P+LA+FVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDL 
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKTQTMEKIKT +LEGNKGF+FLG+PLPENL NDSSVEEYC+KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540
           WHYHGGCLV KVVDGNYKVIGI+NLRVVDGSTF +SPGTNPMATLMMLGRYVGLKVLQQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SSVN 545
           SSVN
Sbjct: 541 SSVN 544

BLAST of PI0013011 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 2.4e-149
Identity = 264/544 (48.53%), Postives = 372/544 (68.38%), Query Frame = 0

Query: 7   TNLILSLMISIFQLRLHSSHAIP--NQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCP 66
           T L++ L++ +    +H S+A P  N+   +M+F+ NATD  +++ YDYII+GGGTAGCP
Sbjct: 12  TALVVLLLLGV----VHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCP 71

Query: 67  LATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGVENIRG 126
           LA TLS  F VLLLERG  P   P+V++  G L      ++  +P Q F+SE+GV N RG
Sbjct: 72  LAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARG 131

Query: 127 RVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRS 186
           RVLGG S +NAGFYSR  ++FF ++G+ WD+  V ++YEWVE  +V +P L TWQ+A R 
Sbjct: 132 RVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRD 191

Query: 187 SLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRI 246
           +LLE GV P NGF + H VGTK GGS FD  G RH + +LL  A  +NI+VA+ ATV+R+
Sbjct: 192 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 251

Query: 247 LF--------SGLSANGVLYSDSKGKIHTAFIRKKGEIILSAGAIGSPQLLHLSGIGPKS 306
           L         S +SA GV+Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSGIGP+S
Sbjct: 252 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 311

Query: 307 HLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPL 366
           +LS+  +PV L QP+VG  + DNPR G +I+ P P+  S ++VVG+ +D  ++++ ++ +
Sbjct: 312 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 371

Query: 367 PILIPPIFSLLPPQATSIIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHP 426
           P   P     +   A+ +   +   + K     S G LRL +ST+V+ +P+VRFNY+S P
Sbjct: 372 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDP 431

Query: 427 DDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKT 486
            DL RCV G RK+G++L+++ M+    +   GN+ F F+G PLP +  ND  + ++C++T
Sbjct: 432 QDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRT 491

Query: 487 VTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKV 541
           V+T WHYHGG +V KVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+GLK+
Sbjct: 492 VSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKM 550

BLAST of PI0013011 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 5.9e-119
Identity = 253/574 (44.08%), Postives = 335/574 (58.36%), Query Frame = 0

Query: 11  LSLMISIFQLRLHSSHAIPNQDFSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLS 70
           L + +S+F   LHS     ++  +Y  F+ +AT  PT   YDYIIIGGGTAGCPLA TLS
Sbjct: 10  LFVALSLF---LHSPICSSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLS 69

Query: 71  SKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG---KNPFQRFVSEDGVENIRGRVL 130
              SVLLLERG  P   P++      L+ F A        +P QRFVSEDGV N R RVL
Sbjct: 70  QNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSEDGVINARARVL 129

Query: 131 GGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNTWQSAFRSSLL 190
           GGGS +NAGFY+R   ++  + G  WD  L  ++Y+WVE  V  QP +  WQ+A R  LL
Sbjct: 130 GGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLL 189

Query: 191 EAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFS 250
           EAG+VP+NGF   H+ GTK GG+IFD  GNRH A +LL  A+P  I V + ATV RILF 
Sbjct: 190 EAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFR 249

Query: 251 GLS-----ANGVLYSDSKGKIHTAFIRK--KGEIILSAGAIGSPQLLHLSGIGPKSHLSS 310
                   ANGV+Y D  G+ H A++++    EIILSAG +GSPQLL LSG+GP + L +
Sbjct: 250 TRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQA 309

Query: 311 LKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQ---------- 370
             + VV+ QP+VGQ M DNP     +  P PV  S ++VVGI  +  Y++          
Sbjct: 310 QNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGG 369

Query: 371 -------SIASPLPILIPPIFSLLPPQATSIIPTLAIFVG-----KFSEVHSEGSLRLNS 430
                  S       +  P  +LL   + + + +   F G     K     S G L L  
Sbjct: 370 GGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-K 429

Query: 431 STNVKKSPIVRFNYYSHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFEF---L 490
           + N K +PIV FNY+ HPDDL RCV+G++ +  +++++   + K  ++     FE+   L
Sbjct: 430 TRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNL 489

Query: 491 GVPLPENLWN---------DSSVEEYCKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLR 541
               P NL             S EE+C+ TVTT WHYHGGC+V +VVDG+YKVIGI  LR
Sbjct: 490 TASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLR 549

BLAST of PI0013011 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 407.9 bits (1047), Expect = 1.3e-113
Identity = 243/537 (45.25%), Postives = 316/537 (58.85%), Query Frame = 0

Query: 51  YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNVFAAGDDGKN 110
           YDYI+IGGGTAGCPLA TLS  FSVL+LERG  P  N   S L      ++  A     +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 111 PFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEET 170
             Q FVS DGV N R RVLGGGS +NAGFYSR    F   AG  WD +LV+++Y WVE  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 171 VVSQPILNTWQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKGNRHGAVELLNKA 230
           +V QP L  WQ A R SLLE GV P NGF   H+ GTK GG+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 231 NPTNIKVAIEATVQRILF--SGL--SANGVLYSDSKGKIHTAFI--RKKGEIILSAGAIG 290
           NP  ++V I ATVQ+I+F  SG      GV++ D KG  H A +  RK  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 291 SPQLLHLSGIGPKSHLSSLKVPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGI 350
           SPQ+L LSGIGPK  L  LK+PVVL   +VG+ M+DNP     +    P+  S ++ VGI
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 351 LQDNIYI----------QSIASPLPILI--PPIFSLLP-----PQATSIIPTL------- 410
            +  +Y+          +SI +   I+     +FS +P     P+AT    T        
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 411 ----AIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCVKGVRKMGDLLK 470
               +  + K +   S G L L  +TNV  +P V FNY+ HP DL RCV+ +R +  ++ 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVT 482

Query: 471 T-----------QTMEKIKTQNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHY 530
           +           Q + K+ + +++ N          P+ L +  S+ ++CK TV T WHY
Sbjct: 483 SNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIWHY 542

Query: 531 HGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 541
           HGGCLV KVV  N KV+G+  LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L++R
Sbjct: 543 HGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of PI0013011 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.4e-112
Identity = 242/541 (44.73%), Postives = 320/541 (59.15%), Query Frame = 0

Query: 37  KFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 96
           +F+ +AT  P    +DYIIIGGGTAGC LA TLS   +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 97  LNVFAAGDDGKNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDME 156
            N         +  Q F+SEDGV N R RVLGGG+++NAGFYSR   +F A AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 157 LVEKAYEWVEETVVSQPILNTWQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDNKG 216
            VE AYEWVE+ VV +P +N WQSAFR  LLEAGV P NGF   H+VGTK GG+IFD  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 217 NRHGAVELLNKANPTNIKVAIEATVQRILFS-----GLSANGVLYSDSKGKIHTAFIRKK 276
           +RH A  LL  ANP  I V + A+V +ILF+        A GV++ D+ G  + A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 277 ----GEIILSAGAIGSPQLLHLSGIGPKSHLSSLKV-PVVLHQPYVGQSMSDNPRFGTNI 336
                E+ILSAGAI SPQLL LSG+GP +HL++ +V PV++ QP VGQ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 337 ILPFPVLPSSVKVVGILQDNIYIQ-SIASPLPILIPPIF----------SLLPPQATS-- 396
             P PV  S V+ VGI +   YI+   A  L I +   F          + LP Q+ S  
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 397 ---------IIPTLAIFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLGRCVK 456
                    +     + + K +   S G L L  +TN   +P V FNY+  P+DL +CV+
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 457 GVRKMGDLLKTQTMEKIKTQNLEGNKGFEFLGVPLPENL-----WNDSSVEEYCKKTVTT 516
           G+  +  ++ ++   K K   L   +G   L + LP NL      +   +E+YC  TV T
Sbjct: 452 GLSTIIKVIDSKGYSKYK-YPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMT 511

Query: 517 YWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQ 541
            +HYHGGC V KVVD NYKV+G+  LR++DGSTF +SPGTNP AT+MMLGRY+G K+L++
Sbjct: 512 IYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGRYMGQKILRE 567

BLAST of PI0013011 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 6.5e-110
Identity = 236/555 (42.52%), Postives = 319/555 (57.48%), Query Frame = 0

Query: 35  YMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQ 94
           Y  F+ +AT  P    +DYIIIGGGT+GC LA TLS   SVL+LERG  P   P+  + +
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 95  GLLNVFAAGDDGKNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWD 154
                  +    K+  Q F+SEDGV N R RVLGGGS++NAGFY+R   E+      +W 
Sbjct: 90  NFATTL-SNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE--TEWK 149

Query: 155 MELVEKAYEWVEETVVSQPILNTWQSAFRSSLLEAGVVPDNGFDVRHLVGTKTGGSIFDN 214
            + VE AYEWVE+ V  QP +  WQ+AF+  LLEAG  P NGF   H+ GTK GG+IFD 
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDR 209

Query: 215 KGNRHGAVELLNKANPTNIKVAIEATVQRILFSGL-----SANGVLYSDSKGKIHTAFIR 274
            G+RH A +LL  ANP NI V + A+V +ILF+        A GV++ D+ G +H A + 
Sbjct: 210 AGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELE 269

Query: 275 KK--GEIILSAGAIGSPQLLHLSGIGPKSHLSSLKV-PVVLHQPYVGQSMSDNPRFGTNI 334
           K    E+ILSAGAIGSPQLL LSGIGP +HL++  + P+VL  P VGQ M DNP     I
Sbjct: 270 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFI 329

Query: 335 ILPFPVLPSSVKVVGILQDNIYIQSIASPL------PILIPPIFSLLPPQATS-----II 394
             P PV  S ++VVGI +   YI+  +  +            + + L    TS       
Sbjct: 330 PSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTS 389

Query: 395 PTLA----------------------IFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYY 454
           PTL+                      + + K +   S G L L  +TN   +P VRFNYY
Sbjct: 390 PTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFNYY 449

Query: 455 SHPDDLGRCVKGVRKMGDLLKTQTMEKIKTQNLEGNKGFE-FLGVPL---PENLWNDSSV 514
             P+DL  CV+G+  +  ++ ++   K K  +   +   +  L VP    P ++ +  ++
Sbjct: 450 QEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNL 509

Query: 515 EEYCKKTVTTYWHYHGGCLVEKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLG 545
            ++C  TV T WHYHGGC V +VVD NY+V+GI +LRV+DGSTF +SPGTNP AT+MMLG
Sbjct: 510 RQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLG 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527062.1e-16152.13(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K24.3e-15950.91(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527071.0e-15549.82(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O827841.8e-15249.55(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
O242431.9e-15149.36(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LK769.0e-29395.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1[more]
A0A1S3BSB53.0e-28891.73(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=... [more]
A0A5A7VNU14.2e-28292.26(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BS352.2e-27890.21(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493093 PE=3 SV=... [more]
A0A5A7VGR69.1e-27790.38(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
XP_011650116.21.7e-29894.84(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
KAE8651660.12.9e-29395.09hypothetical protein Csa_021193 [Cucumis sativus][more]
XP_008452041.16.2e-28891.73PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
KAA0067281.18.7e-28292.26(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa][more]
XP_038889315.12.0e-27887.87(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G73050.12.4e-14948.53Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.15.9e-11944.08Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.11.3e-11345.25Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.11.4e-11244.73Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.16.5e-11042.52Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 389..530
e-value: 1.9E-27
score: 96.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 51..320
e-value: 2.7E-32
score: 112.4
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 124..147
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 279..293
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 51..529
e-value: 2.0E-171
score: 573.2
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 31..539
NoneNo IPR availableGENE3D3.30.410.40coord: 171..479
e-value: 2.0E-171
score: 573.2
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 6..542
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 6..542
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 389..486
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 31..340
e-value: 2.2E-58
score: 196.4
coord: 368..544
e-value: 1.9E-29
score: 100.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013011.1PI0013011.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors