PI0012887 (gene) Melon (PI 482460) v1

Overview
NamePI0012887
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr09: 20982652 .. 20983665 (-)
RNA-Seq ExpressionPI0012887
SyntenyPI0012887
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTTCGTCCAGTGTTTGTTCCGAAATCTCAACTCCACAACTTCCATGGAAGCACAGGATCACTCTTCGATTCGCCGCTCTACTCTTCAACGCTTCTCTCCGATCAGATCTCACCGTTAATCGCCGACTTCTCAATTTCATCGATCCCAAAATCCCTCCCAATTCAACCCACTCCATTTCCTCTTCCGATCTCACTATCCACACCTCTCGTGACCTCTTCCTTCGAATATTCGCCCCCACCCCCACCGCCGCACCCGATGAATCTCTCCCACCGCTGCCGATCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGCTCCGCTGACTCCACTTCATTTGACATGGCTGCCCGCCGCTTCGCCAAAGAATTACACGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGATTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATTGACGAAATGGACGACGACAGTTTACTAGATAGGGTAGATCCGAGTCGTTGCTTTATATTGGGGGAGAGCGCCGGTGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAAAGAGTGAAGATTATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAATATGACAGATTGGTTTTGGAGGGCATTTCTGCCGGCCGGGGAAAATCGAGACCATGCAGCGGCGAATGTGAATGGACCCAATGGAAGGGATATCTCGGGATTGGAAAAATTCCCGGCGACGGTGATTTTCGCCGGAGGATTGGATTTGCTGATTGATCGACACAAGTGTTATTATGAGGGGTTGAAGAGAATGGGTAAGGATGTGAAGCTGGTGGTGTTTTCGAATGCCGTCCATGGATTCTTTGGCTTCTCTGATCTGCCGGAATATTCTTTGATGATTAAGGACATGAGGGATTTCATTGCAAAGCTCACGTAG

mRNA sequence

ATGTGTTCGTCCAGTGTTTGTTCCGAAATCTCAACTCCACAACTTCCATGGAAGCACAGGATCACTCTTCGATTCGCCGCTCTACTCTTCAACGCTTCTCTCCGATCAGATCTCACCGTTAATCGCCGACTTCTCAATTTCATCGATCCCAAAATCCCTCCCAATTCAACCCACTCCATTTCCTCTTCCGATCTCACTATCCACACCTCTCGTGACCTCTTCCTTCGAATATTCGCCCCCACCCCCACCGCCGCACCCGATGAATCTCTCCCACCGCTGCCGATCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGCTCCGCTGACTCCACTTCATTTGACATGGCTGCCCGCCGCTTCGCCAAAGAATTACACGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGATTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATTGACGAAATGGACGACGACAGTTTACTAGATAGGGTAGATCCGAGTCGTTGCTTTATATTGGGGGAGAGCGCCGGTGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAAAGAGTGAAGATTATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAATATGACAGATTGGTTTTGGAGGGCATTTCTGCCGGCCGGGGAAAATCGAGACCATGCAGCGGCGAATGTGAATGGACCCAATGGAAGGGATATCTCGGGATTGGAAAAATTCCCGGCGACGGTGATTTTCGCCGGAGGATTGGATTTGCTGATTGATCGACACAAGTGTTATTATGAGGGGTTGAAGAGAATGGGTAAGGATGTGAAGCTGGTGGTGTTTTCGAATGCCGTCCATGGATTCTTTGGCTTCTCTGATCTGCCGGAATATTCTTTGATGATTAAGGACATGAGGGATTTCATTGCAAAGCTCACGTAG

Coding sequence (CDS)

ATGTGTTCGTCCAGTGTTTGTTCCGAAATCTCAACTCCACAACTTCCATGGAAGCACAGGATCACTCTTCGATTCGCCGCTCTACTCTTCAACGCTTCTCTCCGATCAGATCTCACCGTTAATCGCCGACTTCTCAATTTCATCGATCCCAAAATCCCTCCCAATTCAACCCACTCCATTTCCTCTTCCGATCTCACTATCCACACCTCTCGTGACCTCTTCCTTCGAATATTCGCCCCCACCCCCACCGCCGCACCCGATGAATCTCTCCCACCGCTGCCGATCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGCTCCGCTGACTCCACTTCATTTGACATGGCTGCCCGCCGCTTCGCCAAAGAATTACACGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGATTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATTGACGAAATGGACGACGACAGTTTACTAGATAGGGTAGATCCGAGTCGTTGCTTTATATTGGGGGAGAGCGCCGGTGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAAAGAGTGAAGATTATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAATATGACAGATTGGTTTTGGAGGGCATTTCTGCCGGCCGGGGAAAATCGAGACCATGCAGCGGCGAATGTGAATGGACCCAATGGAAGGGATATCTCGGGATTGGAAAAATTCCCGGCGACGGTGATTTTCGCCGGAGGATTGGATTTGCTGATTGATCGACACAAGTGTTATTATGAGGGGTTGAAGAGAATGGGTAAGGATGTGAAGCTGGTGGTGTTTTCGAATGCCGTCCATGGATTCTTTGGCTTCTCTGATCTGCCGGAATATTCTTTGATGATTAAGGACATGAGGGATTTCATTGCAAAGCTCACGTAG

Protein sequence

MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPNSTHSISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAKLT
Homology
BLAST of PI0012887 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.0e-75
Identity = 149/324 (45.99%), Postives = 202/324 (62.35%), Query Frame = 0

Query: 15  LPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STHSISSSDLTIHTSRD 74
           LP K RI L   + + + + R D T+NRR L   D + PPN    + +S+SD  +  SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISV 134
           L+ R++  TP  + D+    +P++ +FHGGGFAF S ++  +D   RRFA++L A VISV
Sbjct: 73  LWFRLY--TPHVSGDK----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGESAGGNLGHHVAV-- 194
           NYRLAP+ R+P QYDDGFDALK+I+E     L    D SRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENR 254
            R     F  VK+IG I+ QPFFGGEERTE+E +L     ++ + TDW W+A    G NR
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNA 314
           DH A NV GPN  DISGL+ +P T++   G D L D  + YYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 VHGFFGFSDLPEYSLMIKDMRDFI 334
            H F+ F +LPE   +I  ++DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of PI0012887 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.6e-57
Identity = 124/317 (39.12%), Postives = 181/317 (57.10%), Query Frame = 0

Query: 28  LLFNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAP----T 87
           L +N   R D T NR L  F+D K+P N+   + + S D+ I    +L  R++ P    T
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  PTAAPDESLPP----LPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNYRLA 147
             +  D   P     +P+I +FHGG FA  SA+S  +D   RR      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPS-RCFILGESAGGNLGHHVAVRASEYE 207
           P+ R+PC YDDG+  LK+++      L  + D   R F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANV 267
             R+ ++G I   P FGG ERTESE RL  +  +T+   DW+WRAFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNAVHGFFGF 327
            GP  + + GL  FP +++   GLDL+ D    Y EGLK+ G++VKL+    A  GF+  
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 SDLPEYSLMIKDMRDFI 334
            +   +  ++ ++  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of PI0012887 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 127/326 (38.96%), Postives = 178/326 (54.60%), Query Frame = 0

Query: 28  LLFNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAPTPTAA 87
           L +N   R+D T  R L  ++D ++P N+     +SS D  I  S  L +RI+       
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  PDE-----------------SLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAV 147
            +E                 +  P P+I +FHGG F   SA ST +D   RRF K    V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVD-PSRCFILGESAGGNLGHH 207
           V+SVNYR AP+ R+PC YDDG+ ALK++  M    +    D  +R F+ G+S+GGN+ HH
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHH 208

Query: 208 VAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGE 267
           VAVRA++   + VK+ G I     FGG ERTESE RL  +  +TL   DW+W+A+LP   
Sbjct: 209 VAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDA 268

Query: 268 NRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFS 327
           +RDH A N  GPNGR + GL  F  ++I   GLDL  DR   Y + L+  G  VK+V   
Sbjct: 269 DRDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCE 328

Query: 328 NAVHGFFGFSDLPEYSLMIKDMRDFI 334
           NA  GF+   +   Y  +++++ DF+
Sbjct: 329 NATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of PI0012887 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 114/301 (37.87%), Postives = 166/301 (55.15%), Query Frame = 0

Query: 30  FNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAPTPTAAPD 89
           +N   R D T NR L  ++D K+  N+     + S D+ I    +L  R++ P   A  D
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRP---AYAD 90

Query: 90  ESLPP-------------LPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNY 149
           +  PP             +P+I +FHGG FA  SA+S  +D   RR       VV+SVNY
Sbjct: 91  QEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNY 150

Query: 150 RLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPS-RCFILGESAGGNLGHHVAVRAS 209
           R AP+  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA 
Sbjct: 151 RRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAG 210

Query: 210 EYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAA 269
           E     + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GE+R+H A
Sbjct: 211 E---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPA 270

Query: 270 ANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNAVHGF 315
            N   P G+ + G+  FP +++   GLDL+ D    Y EGLK+ G++VKL+    A  GF
Sbjct: 271 CNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGF 322

BLAST of PI0012887 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.2e-51
Identity = 111/324 (34.26%), Postives = 177/324 (54.63%), Query Frame = 0

Query: 28  LLFNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAPT---- 87
           L +    R D + NR L  F+D K+P NS     + S D  + ++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  -------PTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNY 147
                      P  +   +P++ +FHGG F   SA+S  +D   RR       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPDFRFPCQYDDGFDALKFID-----EMDDDSLLDRVDPSRCFILGESAGGNLGHHVA 207
           R +P+ R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNV------YVYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GE+R
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNA 327
           DH A N  GP G+ + G+  FP +++   GLDL+ D    Y +GLK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 VHGFFGFSDLPEYSLMIKDMRDFI 334
             GF+   +   +  +++++  F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of PI0012887 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 302/337 (89.61%), Postives = 317/337 (94.07%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STH 60
           MCSS VC +ISTPQLPWKHRITLRFA LL+N SLRSDLTVNRRLLNFIDPKIP N  STH
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDL + TSRDLFLRIF PTPTAA DESLP LPIIFYFHGGGF FGSAD+TS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           RRFAK+L AVVISVNYRLAP+FRFPCQYDDGFDALKFIDEMDDDSLL+RVD S CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHAAANVNGPNGRDISGLE FPAT+IFAGGLDLLIDR KCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAK 336
           KDVKLVVFSNAVHG+FGF+DLPEYSLMIK+MRDFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0012887 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.5e-173
Identity = 300/337 (89.02%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STH 60
           MCSS VC +ISTPQLPWKHRITLRFA LL+N SLRSDLTVNRRLLNFIDPKIP N  STH
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDL + TSRDLFLRIF PTPTAA DESLP LPIIFYFHGGGF FGSAD+TS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           RRFAK+L AVVISVNYRLAP+FRFPCQ DDGFD LKFIDEMDDDSLL+RVD SRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHA ANVNGPNGRDISGLE FPAT+IFAGGLDLLIDR KCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAK 336
           KDVKLVVFSNAVHG+FGF+DLPEYSLMIK+MRDFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0012887 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.0e-170
Identity = 297/338 (87.87%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTH 60
           M SSSVC E+STPQLPWKH++TLRFA LLFN SLRSD TVNRRLL F+DPKIP  PNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDLTI TSRDLFLRIF P PTAA DESLP LPIIFYFHGGGFAFGSAD+TS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           R FA++L AVVISVNYRLAP+FRFPCQYDDGFDALKFIDEMDDDSLL+RVD SRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHAAANVNGPNGRDISGLE FPATVIFAGGLDLL+DR K YYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAKL 337
           KDVKLVVFSNA HGFFGF DLPEYSLMI++M DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of PI0012887 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 276/337 (81.90%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTH 60
           M  SSV SEIST QLPWKHR+TLRFAALLFNAS+RSDLTVNRRLLNF+DPKIP  PNS H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           ++SSSDLTI  SRDLFLRIF PT  AA D  LP  P+IFYFHGGGFAFGSAD+TS DMAA
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSADATSMDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDS-LLDRVDPSRCFILG 180
           RRF+KEL AVVISVNYRLAP FRFPCQYDDGFDA+KFIDEMDDD  LL + D SRCFILG
Sbjct: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGGEERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240

Query: 241 FWRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRM 300
           FW+AFLPAGE+RDHAAANVNGPNGRD+SGL  FPAT+I AGGLDLLIDR + YYE LKRM
Sbjct: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRM 300

Query: 301 GKDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIA 335
           GK+VKLVVF+N VHGFF F D PEYSLMIK+MRDFIA
Sbjct: 301 GKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of PI0012887 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.3e-153
Identity = 273/332 (82.23%), Postives = 292/332 (87.95%), Query Frame = 0

Query: 5   SVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTHSISS 64
           SV SEIST QLPWKHR+TLRFAALLFNAS+RSDLTVNRRLLNF+DPKIP  PNS H++SS
Sbjct: 5   SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSS 64

Query: 65  SDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFA 124
           SDLTI  SRDLFLRIF PT  AA D  LP  P+IFYFHGGGFAFGSA +TS DMAARRF+
Sbjct: 65  SDLTIDASRDLFLRIFTPT-VAATDSLLP--PVIFYFHGGGFAFGSAAATSMDMAARRFS 124

Query: 125 KELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDD-DSLLDRVDPSRCFILGESAG 184
           KEL AVVISVNYRL+P FRFPCQYDDGFDA+KFIDEMDD D LL R D SRCFILGESAG
Sbjct: 125 KELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESAG 184

Query: 185 GNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 244
           GNLGHHVAVRASEY FKRVK+IGFIASQPFFGGEERTESE RLC Q PLTL + DWFW+A
Sbjct: 185 GNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKA 244

Query: 245 FLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDV 304
           FLPAGE+RDHAAANVNGPNGRD+SGL  FPAT+I AGGLD LIDR + YYE LKRMGKDV
Sbjct: 245 FLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKDV 304

Query: 305 KLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFI 334
           KLVVF+N VHGFF F DLPEYSLMIK+MRDFI
Sbjct: 305 KLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of PI0012887 vs. NCBI nr
Match: XP_008459713.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 621.3 bits (1601), Expect = 4.8e-174
Identity = 302/337 (89.61%), Postives = 317/337 (94.07%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STH 60
           MCSS VC +ISTPQLPWKHRITLRFA LL+N SLRSDLTVNRRLLNFIDPKIP N  STH
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDL + TSRDLFLRIF PTPTAA DESLP LPIIFYFHGGGF FGSAD+TS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           RRFAK+L AVVISVNYRLAP+FRFPCQYDDGFDALKFIDEMDDDSLL+RVD S CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHAAANVNGPNGRDISGLE FPAT+IFAGGLDLLIDR KCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAK 336
           KDVKLVVFSNAVHG+FGF+DLPEYSLMIK+MRDFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0012887 vs. NCBI nr
Match: KAA0039340.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 616.3 bits (1588), Expect = 1.6e-172
Identity = 300/337 (89.02%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STH 60
           MCSS VC +ISTPQLPWKHRITLRFA LL+N SLRSDLTVNRRLLNFIDPKIP N  STH
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDL + TSRDLFLRIF PTPTAA DESLP LPIIFYFHGGGF FGSAD+TS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           RRFAK+L AVVISVNYRLAP+FRFPCQ DDGFD LKFIDEMDDDSLL+RVD SRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHA ANVNGPNGRDISGLE FPAT+IFAGGLDLLIDR KCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAK 336
           KDVKLVVFSNAVHG+FGF+DLPEYSLMIK+MRDFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0012887 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 297/338 (87.87%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTH 60
           M SSSVC E+STPQLPWKH++TLRFA LLFN SLRSD TVNRRLL F+DPKIP  PNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           S+SSSDLTI TSRDLFLRIF P PTAA DESLP LPIIFYFHGGGFAFGSAD+TS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGE 180
           R FA++L AVVISVNYRLAP+FRFPCQYDDGFDALKFIDEMDDDSLL+RVD SRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGG+ERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMG 300
           WRAFLPAGE+RDHAAANVNGPNGRDISGLE FPATVIFAGGLDLL+DR K YYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIAKL 337
           KDVKLVVFSNA HGFFGF DLPEYSLMI++M DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of PI0012887 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 555.4 bits (1430), Expect = 3.3e-154
Identity = 276/337 (81.90%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MCSSSVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTH 60
           M  SSV SEIST QLPWKHR+TLRFAALLFNAS+RSDLTVNRRLLNF+DPKIP  PNS H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  SISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAA 120
           ++SSSDLTI  SRDLFLRIF PT  AA D  LP  P+IFYFHGGGFAFGSAD+TS DMAA
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSADATSMDMAA 120

Query: 121 RRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDS-LLDRVDPSRCFILG 180
           RRF+KEL AVVISVNYRLAP FRFPCQYDDGFDA+KFIDEMDDD  LL + D SRCFILG
Sbjct: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGGEERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240

Query: 241 FWRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRM 300
           FW+AFLPAGE+RDHAAANVNGPNGRD+SGL  FPAT+I AGGLDLLIDR + YYE LKRM
Sbjct: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRM 300

Query: 301 GKDVKLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIA 335
           GK+VKLVVF+N VHGFF F D PEYSLMIK+MRDFIA
Sbjct: 301 GKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of PI0012887 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-153
Identity = 273/333 (81.98%), Postives = 294/333 (88.29%), Query Frame = 0

Query: 5   SVCSEISTPQLPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIP--PNSTHSISS 64
           SV SEIST QLPWKHR+TLRFAALLFNAS+RSDLTVNRRLLNF+DPKIP  PNS H++SS
Sbjct: 5   SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSS 64

Query: 65  SDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFA 124
           SDLTI  SRDLFLRIF PT  AA D  LP  P+IFYFHGGGFAFGSA +TS DMAARRF+
Sbjct: 65  SDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAARRFS 124

Query: 125 KELHAVVISVNYRLAPDFRFPCQYDDGFDALKFIDEMDDDS-LLDRVDPSRCFILGESAG 184
           KEL AVVISVNYRLAP FRFPCQYDDGFDA+KFIDEMDDD  LL + D SRCFILGESAG
Sbjct: 125 KELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAG 184

Query: 185 GNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 244
           GNLGHHVAVRASEY FKRVK+IGFIASQPFFGGEERTESE RLC Q PLTL + DWFW+A
Sbjct: 185 GNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKA 244

Query: 245 FLPAGENRDHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDV 304
           FLPAGE+RDHAAANVNGPNGRD+SGL  FPAT+I AGGLD+LIDR + YYE LKRMGK+V
Sbjct: 245 FLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEV 304

Query: 305 KLVVFSNAVHGFFGFSDLPEYSLMIKDMRDFIA 335
           KLVVF+N VHGFF F DLPEYSLMIK+MRDFIA
Sbjct: 305 KLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 334

BLAST of PI0012887 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-76
Identity = 149/324 (45.99%), Postives = 202/324 (62.35%), Query Frame = 0

Query: 15  LPWKHRITLRFAALLFNASLRSDLTVNRRLLNFIDPKIPPN--STHSISSSDLTIHTSRD 74
           LP K RI L   + + + + R D T+NRR L   D + PPN    + +S+SD  +  SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFAPTPTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISV 134
           L+ R++  TP  + D+    +P++ +FHGGGFAF S ++  +D   RRFA++L A VISV
Sbjct: 73  LWFRLY--TPHVSGDK----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPSRCFILGESAGGNLGHHVAV-- 194
           NYRLAP+ R+P QYDDGFDALK+I+E     L    D SRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENR 254
            R     F  VK+IG I+ QPFFGGEERTE+E +L     ++ + TDW W+A    G NR
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNA 314
           DH A NV GPN  DISGL+ +P T++   G D L D  + YYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 VHGFFGFSDLPEYSLMIKDMRDFI 334
            H F+ F +LPE   +I  ++DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of PI0012887 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 6.1e-58
Identity = 124/317 (39.12%), Postives = 181/317 (57.10%), Query Frame = 0

Query: 28  LLFNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAP----T 87
           L +N   R D T NR L  F+D K+P N+   + + S D+ I    +L  R++ P    T
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  PTAAPDESLPP----LPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNYRLA 147
             +  D   P     +P+I +FHGG FA  SA+S  +D   RR      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPS-RCFILGESAGGNLGHHVAVRASEYE 207
           P+ R+PC YDDG+  LK+++      L  + D   R F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANV 267
             R+ ++G I   P FGG ERTESE RL  +  +T+   DW+WRAFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNAVHGFFGF 327
            GP  + + GL  FP +++   GLDL+ D    Y EGLK+ G++VKL+    A  GF+  
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 SDLPEYSLMIKDMRDFI 334
            +   +  ++ ++  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of PI0012887 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 114/301 (37.87%), Postives = 166/301 (55.15%), Query Frame = 0

Query: 30  FNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAPTPTAAPD 89
           +N   R D T NR L  ++D K+  N+     + S D+ I    +L  R++ P   A  D
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRP---AYAD 90

Query: 90  ESLPP-------------LPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNY 149
           +  PP             +P+I +FHGG FA  SA+S  +D   RR       VV+SVNY
Sbjct: 91  QEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNY 150

Query: 150 RLAPDFRFPCQYDDGFDALKFIDEMDDDSLLDRVDPS-RCFILGESAGGNLGHHVAVRAS 209
           R AP+  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA 
Sbjct: 151 RRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAG 210

Query: 210 EYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAA 269
           E     + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GE+R+H A
Sbjct: 211 E---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPA 270

Query: 270 ANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNAVHGF 315
            N   P G+ + G+  FP +++   GLDL+ D    Y EGLK+ G++VKL+    A  GF
Sbjct: 271 CNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGF 322

BLAST of PI0012887 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.2e-52
Identity = 111/324 (34.26%), Postives = 177/324 (54.63%), Query Frame = 0

Query: 28  LLFNASLRSDLTVNRRLLNFIDPKIPPNS--THSISSSDLTIHTSRDLFLRIFAPT---- 87
           L +    R D + NR L  F+D K+P NS     + S D  + ++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  -------PTAAPDESLPPLPIIFYFHGGGFAFGSADSTSFDMAARRFAKELHAVVISVNY 147
                      P  +   +P++ +FHGG F   SA+S  +D   RR       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPDFRFPCQYDDGFDALKFID-----EMDDDSLLDRVDPSRCFILGESAGGNLGHHVA 207
           R +P+ R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNV------YVYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GE+R
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAAANVNGPNGRDISGLEKFPATVIFAGGLDLLIDRHKCYYEGLKRMGKDVKLVVFSNA 327
           DH A N  GP G+ + G+  FP +++   GLDL+ D    Y +GLK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 VHGFFGFSDLPEYSLMIKDMRDFI 334
             GF+   +   +  +++++  F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of PI0012887 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 8.6e-44
Identity = 104/280 (37.14%), Postives = 150/280 (53.57%), Query Frame = 0

Query: 45  LNFIDPKIPPNSTHSISSSDLTIHTSRDLFLRIFAPTPTAAPDESLPPLPIIFYFHGGGF 104
           ++ I  +IP  +  ++   D   H   +L LR++ P   +    +   LP++ +FHGGGF
Sbjct: 32  IDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISAS----NRTALPVVVFFHGGGF 91

Query: 105 AFGSADSTSFDMAARRFAKELHAVVISVNYRLAPDFRFPCQYDDGFDALKFI-DEMDDDS 164
            FGS     F       A  L+A+V+S +YRLAP+ R P  ++D    L ++ D+   D 
Sbjct: 92  CFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDG 151

Query: 165 LL------DRVDPSRCFILGESAGGNLGHHVAVR--ASEYEFKRVKIIGFIASQPFFGGE 224
           +         VD  R F++G+S+GGN+ H +AVR  +   E   V++ G++   PFFGGE
Sbjct: 152 VNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGE 211

Query: 225 ERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGLEKFPATVI 284
           ERT SEN   + L L+L++ D FWR  LP G  RDH  AN  GP    +  +   P  VI
Sbjct: 212 ERTNSENGPSEAL-LSLDLLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVI 271

Query: 285 FAGGLDLLIDRHKCYYEGLKRM-GKDVKLVVFSNAVHGFF 315
             GG +LL DR K Y   LK+M GK V  + F N  HGF+
Sbjct: 272 -VGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFY 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT107.0e-7545.99Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G68.6e-5739.12Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5451.1e-5638.96Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA72.9e-5237.87Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC13.2e-5134.26Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CBA42.3e-17489.61probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL917.5e-17389.02Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0KXS86.0e-17087.87Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
A0A6J1ECC81.6e-15481.90probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A6J1IER72.3e-15382.23probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_008459713.14.8e-17489.61PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
KAA0039340.11.6e-17289.02putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative car... [more]
XP_004141618.11.2e-16987.87probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ... [more]
XP_022925491.13.3e-15481.90probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6592331.12.1e-15381.98putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
AT5G23530.15.0e-7645.99carboxyesterase 18 [more]
AT5G27320.16.1e-5839.12alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.12.0e-5337.87alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.12.2e-5234.26alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.18.6e-4437.14alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 96..314
e-value: 8.0E-53
score: 179.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 3..336
e-value: 8.9E-83
score: 280.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 53..335
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 10..334
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 10..334
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 96..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012887.1PI0012887.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity