PI0012741 (gene) Melon (PI 482460) v1

Overview


Name	PI0012741
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Cytochrome P450 81E8-like
Location	chr07: 22764220 .. 22766998 (-)
RNA-Seq Expression	PI0012741
Synteny	PI0012741

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCTCACACAGTTACTTGCTTTTCTCTTTCTCTTCTCTTCGTCACTCTCATTTTTCTTTCCAAGCTCAGAAAAGTAAGAAACCAAAAAAAACTTCCACCAACCCCACCTTCCCTTCCGATAATCGGTCATCTCCATCTCCTCAAAGAACCTTTCCACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCACGTGCTAGTCGTCTCCTCCCCCACCGCAGTCCGTGAATGCTTCACAAAAAACGACATCGTATTCGCCAACCGCCCTCGCTTACTCTCAGGAAAATATATCAATTACAACTACACCGCCGTGGGATTCGCCCCGTACGGACAGCACTGGCGCAACACAACAGAGCTACTCTCCAACCACCGGCTCAACACATATATGAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAATTTGTACAAATGGAGTAGTGGGGGGGATTTTGTTGTGGTGTATATGAAGTCGAAATTGAAGGAACTTTCATTCAATACCGTGATGAGGATGGTTAGTGGGAAAAGGTATTTTGGTGTGGAAGTGGAGGATGTGGAAGAAGGATTGGAATTTAGAGAGATAATGAAGGAACTACTTGAATTGAGTGGGGCTACTAATGCGGCCGACTTTTTTACAATTTTGAAAGTGTTTGATTTTGAAGGAGTTAAGAAGAGGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTGTACAGAAACTTATTGATGAAGAGAGGGAAAGAAGAGTTTCACGTTGGGCGGAGGAAAAACAGAAACAGGGGAAAACGTCTATGATTCAAACTCTGCTTTCCTTGCAAGAATCTCAACCTCATTATTACTCCGATGATATAATCAAGGGTCATGTTCTGGTAAGTGCTAACTTCCTTTAAAAATATCAATCTTACTTTTTCAACCCTTTTGATATATAATTTTGGACTCATTGACCCAACCCAAAGTGAGTTGGATGAAAAACGATAAACATCATCCGTTCAAATATTTATTAAATCAATTATAGTCAGTTGACTTTGAACGACTGATAGATTGTCTCAGATATAATTTATAATCAACCATGTTTCTGTTACCAAGAAAATAACAATATCAAAAAATATAAAAAAAATTTAGATGCATCTATATTTTTTATAATAATAGCATGCAATTTAATCTATATATCCTTTTCTTACCAAAAAAAAATTAAAGATTCAAGTTTAATATCAATTTAACATGTTATCGAAACAAAAGATTTTGGAGGACAAACAAAGAACAAATCCTTGTTATGTCATGTTTTCTCTCTTTTAATATTGATTTTAATCAATTAGCTATTTTATAAAATTTTAAAGTTTATTGCTATAATTTAATGATGAATTTGGTGGGAATGCATAAATATATGCGATGTTCAAGATGTAATAAATATTTTTTAATCAAATTTACCATAACTACTATTTGAAGATTTTGGATTGGTGGGTGGTTTAACATTTTCTTGCTTTGGCAAAATTGATTTTACTTCATTTTAGTAATATTCTATGGTTTGAGAACAACATTATCTTAATTTATTGTTGGCTATAAAATATATTTATGAGAGTAAAGTGGAATATTCATCTTCTTATCGGATTGATTTCAAATATGTGGAAATGAAGTGAAATAATTAGAAAATAGAGTAAAATTCAAATTTTATGAAGGATAAAGTACTATATTTAGGATTAAAGTCAAAGATAATTATACGGCCTATTATGAATATCTTATTATCTTTTAACATCGGGATCTAAAATACGTATGGATTTATTAATATAAATTGCGGTGTCAGTCTTAGACGAGAATATCAATACCTATCAATGCGACAGAAACCTCAAACGACCTTAACTATCCACTTGCCTTCACCCTTAATATTTTCAAATTTTATATGTAAAAATAAAATGATCAATATGTGACCTAAAACAATAATGTTTAAAGTAAGTTTCCAAACTTTATATAGAAATACCAATTAATATTTAGGCTCACAGCTTAAATGGTAATAATTTAGTTTCAATATAAATCTCTAACCATTTAATTTTGTTTAACTTTATGGTGTAATAGTTTTGTATGTAATAGTTTAACTTAAATGAATGATATGTGTGTACAGACGATGTTGGTTGCGGGTACAGACACAACTTCAGAAACAATAGAATAGACAATGTCACTACTTTTGAATCATCCAATGATGATGAAGAAAGCTTGGATTGAGATAAGAGATTATGTTGGAGAAAATTGAATGGTGGAGGAAAGTAATGTGAGTAAACTAAAATATTTAGAAGCTATAATCTACAAGACTCTTCGACTGTTTCCAGCAGTTCCATTGTTGGTCCCACACGAATGCTCTGAAGATTGTAGAATTGAAGGATTTGAGGTACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCTATTCATAGGGATCCGAAAGTGTGGGAGGATCCTACCAACTTTTGACCAGAGAGGTTTCTAAATTGGGAAGGTGTTGATTTCTACGAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTGTCGAGGAATTGCCCTGGCCCAACGATCTATTTGATTGACATTAGCTACTTTGATTCAATGCTGCTTTGAGTGGAGAAAGTTGATGAACATGAACAAATAGACTTGTCTGAAGGAACTGGAATCACTATGCCCAAAGCCAAAGCTTTGGAGGCAATGTGTAAACCTCGTACCTCCATGTTTCATTTGTTGGCTCAACTTTGA

mRNA sequence

ATGGAACCTCACACAGTTACTTGCTTTTCTCTTTCTCTTCTCTTCGTCACTCTCATTTTTCTTTCCAAGCTCAGAAAAGTAAGAAACCAAAAAAAACTTCCACCAACCCCACCTTCCCTTCCGATAATCGGTCATCTCCATCTCCTCAAAGAACCTTTCCACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCACGTGCTAGTCGTCTCCTCCCCCACCGCAGTCCGTGAATGCTTCACAAAAAACGACATCGTATTCGCCAACCGCCCTCGCTTACTCTCAGGAAAATATATCAATTACAACTACACCGCCGTGGGATTCGCCCCGTACGGACAGCACTGGCGCAACACAACAGAGCTACTCTCCAACCACCGGCTCAACACATATATGAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAATTTGTACAAATGGAGTAGTGGGGGGGATTTTGTTGTGGTGTATATGAAGTCGAAATTGAAGGAACTTTCATTCAATACCGTGATGAGGATGGTTAGTGGGAAAAGGTATTTTGGTGTGGAAGTGGAGGATGTGGAAGAAGGATTGGAATTTAGAGAGATAATGAAGGAACTACTTGAATTGAGTGGGGCTACTAATGCGGCCGACTTTTTTACAATTTTGAAAGTGTTTGATTTTGAAGGAGTTAAGAAGAGGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTGTACAGAAACTTATTGATGAAGAGAGGGAAAGAAGAGTTTCACGTTGGGCGGAGGAAAAACAGAAACAGGGGAAAACGTCTATGATTCAAACTCTGCTTTCCTTGCAAGAATCTCAACCTCATTATTACTCCGATGATATAATCAAGGGTCATGTTCTGACAATGTCACTACTTTTGAATCATCCAATGATGATGAAGAAAGCTTGGATTGAGATAAGAGATTATACTCTTCGACTGTTTCCAGCAGTTCCATTGTTGGTCCCACACGAATGCTCTGAAGATTGTAGAATTGAAGGATTTGAGGTACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCTATTCATAGGGATCCGAAAAGGTTTCTAAATTGGGAAGGTGTTGATTTCTACGAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTTGGAGAAAGTTGATGAACATGAACAAATAGACTTGTCTGAAGGAACTGGAATCACTATGCCCAAAGCCAAAGCTTTGGAGGCAATGTGTAAACCTCGTACCTCCATGTTTCATTTGTTGGCTCAACTTTGA

Coding sequence (CDS)

ATGGAACCTCACACAGTTACTTGCTTTTCTCTTTCTCTTCTCTTCGTCACTCTCATTTTTCTTTCCAAGCTCAGAAAAGTAAGAAACCAAAAAAAACTTCCACCAACCCCACCTTCCCTTCCGATAATCGGTCATCTCCATCTCCTCAAAGAACCTTTCCACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCACGTGCTAGTCGTCTCCTCCCCCACCGCAGTCCGTGAATGCTTCACAAAAAACGACATCGTATTCGCCAACCGCCCTCGCTTACTCTCAGGAAAATATATCAATTACAACTACACCGCCGTGGGATTCGCCCCGTACGGACAGCACTGGCGCAACACAACAGAGCTACTCTCCAACCACCGGCTCAACACATATATGAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAATTTGTACAAATGGAGTAGTGGGGGGGATTTTGTTGTGGTGTATATGAAGTCGAAATTGAAGGAACTTTCATTCAATACCGTGATGAGGATGGTTAGTGGGAAAAGGTATTTTGGTGTGGAAGTGGAGGATGTGGAAGAAGGATTGGAATTTAGAGAGATAATGAAGGAACTACTTGAATTGAGTGGGGCTACTAATGCGGCCGACTTTTTTACAATTTTGAAAGTGTTTGATTTTGAAGGAGTTAAGAAGAGGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTGTACAGAAACTTATTGATGAAGAGAGGGAAAGAAGAGTTTCACGTTGGGCGGAGGAAAAACAGAAACAGGGGAAAACGTCTATGATTCAAACTCTGCTTTCCTTGCAAGAATCTCAACCTCATTATTACTCCGATGATATAATCAAGGGTCATGTTCTGACAATGTCACTACTTTTGAATCATCCAATGATGATGAAGAAAGCTTGGATTGAGATAAGAGATTATACTCTTCGACTGTTTCCAGCAGTTCCATTGTTGGTCCCACACGAATGCTCTGAAGATTGTAGAATTGAAGGATTTGAGGTACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCTATTCATAGGGATCCGAAAAGGTTTCTAAATTGGGAAGGTGTTGATTTCTACGAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTTGGAGAAAGTTGATGAACATGAACAAATAGACTTGTCTGAAGGAACTGGAATCACTATGCCCAAAGCCAAAGCTTTGGAGGCAATGTGTAAACCTCGTACCTCCATGTTTCATTTGTTGGCTCAACTTTGA

Protein sequence

MEPHTVTCFSLSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDLSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRNTTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKGHVLTMSLLLNHPMMMKKAWIEIRDYTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRFLNWEGVDFYEWIPFGMGRRALEKVDEHEQIDLSEGTGITMPKAKALEAMCKPRTSMFHLLAQL

Homology

[Close]

BLAST of PI0012741 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.6e-98
Identity = 218/511 (42.66%), Postives = 299/511 (58.51%), Query Frame = 0


Query: 10  SLSLLFVTLIFLSKLRKV----RNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHDLSNKY 69
           +L   F+ ++ LS   K+    R ++ LPP+P  +LP+IGHLHL+ +  HR L+DLS KY
Sbjct: 5   TLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 64

Query: 70  GPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQH 129
           G + SL +G+R VLVVSSP A  ECFTKNDIVFANRP  + GKYI YNYT +  +PYG+H
Sbjct: 65  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 124

Query: 130 WRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSF 189
           WRN       E+ S   LN +++IR +E+K  +  +LY+ SSG DF  V MKSKL ELSF
Sbjct: 125 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLL-SLYQ-SSGQDFGKVEMKSKLSELSF 184

Query: 190 NTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKR 249
           N  MRMV+GKRYFG +V D +E   FR ++ E+ E +GA+N  DF   L+  DF+  +K+
Sbjct: 185 NVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKK 244

Query: 250 MMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIK 309
           + K S   D F+Q+LI E R  +              +MI  LLSLQESQP YY+D IIK
Sbjct: 245 VSKISQEMDAFLQRLIHESRINK-----------NNVTMIDHLLSLQESQPEYYTDQIIK 304

Query: 310 GHVL----------------TMSLLLNHPMMMKKAWIEIR-------------------- 369
           G ++                 MSLLLNHP  ++KA  EI                     
Sbjct: 305 GIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYL 364

Query: 370 ----DYTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRFLNWE--- 429
                 T RL PA P+LVPHE S+DC+++G++VPKGT+L+VNAWAIHRDP+    W+   
Sbjct: 365 HNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPE---FWDEPT 424

Query: 430 --------GVDF--YEWIPFGMGRRAL-----------------------EKVDEHEQID 435
                   GV+    + +PFGMGRR+                        +++ E  +ID
Sbjct: 425 LFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGE-AKID 484

[Close]

BLAST of PI0012741 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 215/504 (42.66%), Postives = 277/504 (54.96%), Query Frame = 0


Query: 6   VTCFSLSLLFVTLIFLSKLRKV-----RNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDL 65
           +T F LSL+ ++L FL    KV     R  K LPP P  LPIIG+LH LK+P H   H L
Sbjct: 1   MTTFYLSLI-ISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTL 60

Query: 66  SNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAP 125
           S KYG I SL  GSR V+VVSS T  +ECFTKNDIV ANRP  L+GKYI YN T V  +P
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 126 YGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLK 185
           YG HWRN     + E+LS+HRLN+++ IR +E+   ++    K  +G  F  V ++    
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNG--FTEVELRPMFS 180

Query: 186 ELSFNTVMRMVSGKRYFG--VEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDF 245
           E++FNT+MRMVSGKRY+G   +V DVEE   FR I+KE++ L GA N  DF   L+ FDF
Sbjct: 181 EMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDF 240

Query: 246 EGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYY 305
           +G++KR+ K S R D F+Q LIDE R            K+   +MI  LL+ Q+SQP YY
Sbjct: 241 DGLEKRLKKISKRTDAFLQGLIDEHR----------FGKRNSNTMIDHLLTQQQSQPEYY 300

Query: 306 SDDIIKGHVLTMSL----------------LLNHPMMMKKAWIEIRDY------------ 365
           +D IIKG ++ M L                LLNHP +MKKA  E+  +            
Sbjct: 301 TDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDI 360

Query: 366 ------------TLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRF- 425
                       TLRL  A PLLVPH  SED  + G+ +P+ T+LMVNAW IHRDP  + 
Sbjct: 361 SKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWS 420

[Close]

BLAST of PI0012741 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 6.9e-91
Identity = 195/499 (39.08%), Postives = 280/499 (56.11%), Query Frame = 0


Query: 6   VTCFSLSLLFVTLIFLSKL-RKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDLSNKY 65
           ++  S S+ ++ L F+  +  + R  K LPP PPSLPIIG+LH LK P HR    LS KY
Sbjct: 4   LSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY 63

Query: 66  GPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQH 125
           G + SL  GSR V+VVSS +  ++CFTKND+V ANRPR LSGKYI YNYT +G   YG+H
Sbjct: 64  GHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 126 WRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSF 185
           WRN       ++LSNHR+N++  IR +E +  +        S  +F  + + S+L +++F
Sbjct: 124 WRNLRRITALDVLSNHRINSFSGIRRDETQRLITR--LADDSSTNFAEMELSSRLYDMTF 183

Query: 186 NTVMRMVSGKRYFG--VEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVK 245
           N +MRM+SGKRY+G   +  D++E  +FR+++ ELL+LSGA N  DF  +L+  DFE ++
Sbjct: 184 NNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLE 243

Query: 246 KRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDI 305
           KR+   SG+ D F++ LI+E R +          K+   +MI  LL+LQ+SQP YY+D I
Sbjct: 244 KRLKDISGKTDAFLRGLIEEHRTK----------KERANTMIDHLLNLQDSQPEYYTDQI 303

Query: 306 IKGHVL----------------TMSLLLNHPMMMKKAWIEIRDY---------------- 365
           IKG  L                +MS LLNHP ++KK   E+  +                
Sbjct: 304 IKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLT 363

Query: 366 --------TLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRFLN--- 425
                   TLRL+   PLL+PH  S++C I G++VP+ T++++NAWAIHRDP+ +     
Sbjct: 364 YLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATT 423

[Close]

BLAST of PI0012741 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 9.9e-90
Identity = 205/494 (41.50%), Postives = 268/494 (54.25%), Query Frame = 0


Query: 11  LSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDLSNKYGPILSL 70
           LSL F+  I +      R  K LPP PP++PIIG+LH LK P HR    LS  YG I SL
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSL 67

Query: 71  TIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRN--- 130
             GSR V+VVSSP+   ECFTKNDI+ ANRPR L+GKYI YNYT +G A YG HWRN   
Sbjct: 68  WFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRR 127

Query: 131 --TTELLSNHRLNTYMNIRVEELKLWVKNNLYK-WSSGGDFVVVYMKSKLKELSFNTVMR 190
             T ++LSN+RLN+++ +R +E    ++  L    S G  F  V ++ +L E++FN +MR
Sbjct: 128 ITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMR 187

Query: 191 MVSGKRYFG--VEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKRMMK 250
           M+SGKRY+G   +V DVEE  +FREI+ E++ L GA N  DF  +L+V D + ++KR  +
Sbjct: 188 MISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKR 247

Query: 251 ASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKGHV 310
            + R++ F++ LI+E R   +          G T MI  LL L ESQP YYSD +IKG +
Sbjct: 248 IAKRSNAFLEGLIEEHRRGNI-------HSDGGT-MIDHLLKLSESQPEYYSDHLIKGLI 307

Query: 311 ----------------LTMSLLLNHPMMMKKAWIE------------------------I 370
                             MS LLNHP ++KKA  E                        I
Sbjct: 308 QGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNI 367

Query: 371 RDYTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK-----------R 424
              TLRL P  PLL+PH  SEDC I  F VPK T+++ N W IHRDPK           R
Sbjct: 368 ISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER 427

[Close]

BLAST of PI0012741 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.5e-87
Identity = 192/494 (38.87%), Postives = 278/494 (56.28%), Query Frame = 0


Query: 12  SLLFVTLIFLSKLR-KVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDLSNKYGPILSL 71
           SL ++++ F+ +L  + R  K LPP P SLPIIG+LH LK P +R    L+ KYG ++SL
Sbjct: 10  SLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISL 69

Query: 72  TIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRN--- 131
             GSR V+VVSS +  +ECFTKND+V ANRPR LSGKYI YNYT +G   YG+HWRN   
Sbjct: 70  WFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 129

Query: 132 --TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSFNTVMRM 191
             + ++LSNHR+N +  IR +E +  +K       S   F  V +  +  +++FN +MRM
Sbjct: 130 ITSLDVLSNHRINNFAPIRRDETQRLIKK--LAEDSSTKFAEVELTFRFFDMTFNNIMRM 189

Query: 192 VSGKRYFG--VEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKRMMKA 251
           +SGKRY+G   ++ +V+E  +FR+++ ELL+LSGA N  DF  +LK  DFE ++KR+ + 
Sbjct: 190 ISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRI 249

Query: 252 SGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKGHVL 311
             + DVF+  L+ E+R +          K+   +MI  LL++QESQP YY+D IIKG  L
Sbjct: 250 GEKNDVFLSGLLQEQRSK----------KERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 309

Query: 312 ----------------TMSLLLNHPMMMKKAWIEIRDY---------------------- 371
                           TMS +LN+P ++KK   E+  +                      
Sbjct: 310 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 369

Query: 372 --TLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRF----------L 426
             TLRL+   PLL+PH  +++C + G++VP+ T++++NAWAIHRDP+ +           
Sbjct: 370 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 429

[Close]

BLAST of PI0012741 vs. ExPASy TrEMBL
Match: A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.6e-212
Identity = 394/515 (76.50%), Postives = 411/515 (79.81%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPHT+T FSLSLL + TLI LSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLH+
Sbjct: 9   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 68

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR VLVVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAVGFA
Sbjct: 69  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 128

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKL 180
           PYGQHWRN     TTELLSNHRLN Y+NIRVEELKLWVKNNLYKW SGGDFVVV MKSKL
Sbjct: 129 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKL 188

Query: 181 KELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFE 240
           KELSFNTVMRM+SGKRYFGVEVEDVEE  EFREIMKELLELSGATNAADF  IL+VFD E
Sbjct: 189 KELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 248

Query: 241 GVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYS 300
           GVKKRMMKASGRADVF+QKLIDEER++RVSRW EEKQKQ KTSMI+TLLSLQESQP YYS
Sbjct: 249 GVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYS 308

Query: 301 DDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD-------------- 360
           DDIIKGHVLT                MSLLLNHPM MKKAWIEIRD              
Sbjct: 309 DDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVS 368

Query: 361 ----------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK---- 420
                      TLR+FPA PLLVPHECSEDC IEGFEVPKGTML+VNAW IHRDPK    
Sbjct: 369 ELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWED 428

Query: 421 -------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHE 436
                  RFLNWEGV+ Y+WIPFGMGRRA                        +KVDE E
Sbjct: 429 PTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQE 488

[Close]

BLAST of PI0012741 vs. ExPASy TrEMBL
Match: A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.7e-209
Identity = 391/515 (75.92%), Postives = 408/515 (79.22%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPHT+T FSLSLL + TLI LSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLH+
Sbjct: 6   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 65

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR VLVVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAVGFA
Sbjct: 66  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 125

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKL 180
           PYGQHWRN     TTELLSNHRLN Y+NIRVEELKLWVKNNLYKW SGGDFVVV MKSKL
Sbjct: 126 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKL 185

Query: 181 KELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFE 240
           KELSFNTVMRM+SGKRYFGVEVEDVEE  EFREIMKELLELSGATNAADF  IL+VFD E
Sbjct: 186 KELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 245

Query: 241 GVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYS 300
           GVKKRMMKASGRADVF+QKLIDEER++   RW EEKQKQ KTSMI+TLLSLQESQP YYS
Sbjct: 246 GVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQESQPQYYS 305

Query: 301 DDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD-------------- 360
           DDIIKGHVLT                MSLLLNHPM MKKAWIEIRD              
Sbjct: 306 DDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVS 365

Query: 361 ----------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK---- 420
                      TLR+FPA PLLVPHECSEDC IEGFEVPKGTML+VNAW IHRDPK    
Sbjct: 366 ELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWED 425

Query: 421 -------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHE 436
                  RFLNWEGV+ Y+WIPFGMGRRA                        +KVDE E
Sbjct: 426 PTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQE 485

[Close]

BLAST of PI0012741 vs. ExPASy TrEMBL
Match: A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 3.1e-163
Identity = 316/514 (61.48%), Postives = 368/514 (71.60%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDL 60
           ME +T+T  SLSLLF TL  + KLRK ++ ++ PP+PPSLP+IGHLHLLK+PFHRVL DL
Sbjct: 1   MEANTITWLSLSLLF-TLSLIYKLRK-QSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQDL 60

Query: 61  SNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAP 120
           S+K+GPILSL+IGSR ++V+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT V  A 
Sbjct: 61  SDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAVAS 120

Query: 121 YGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLK 180
           YG HWRN     TTELLSNHRLN Y+NIRV+ELKLWVKN   +     DFV + M+SKL 
Sbjct: 121 YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSKLN 180

Query: 181 ELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEG 240
           ELSFNTVMRMVSGKRYFGV+VEDVEE  EFREIMKELL LSGA+N ADF  IL++FD++G
Sbjct: 181 ELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDYQG 240

Query: 241 VKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSD 300
           ++KRM+KASGRAD+F+Q LID ERERR SRW EE  K G  +MIQ+LLS QESQP YYSD
Sbjct: 241 LEKRMVKASGRADLFLQNLIDCERERRASRWPEE--KHGNKTMIQSLLSFQESQPQYYSD 300

Query: 301 DIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD--------------- 360
           DIIKGHVLT                MSLLLNHP +M KAW EIR+               
Sbjct: 301 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSS 360

Query: 361 ---------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRD------- 420
                     TLRLFP  PLLVPHE SE+CRIEGF++PKGTML+VNAWAIHRD       
Sbjct: 361 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 420

Query: 421 ----PKRFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHEQ 436
               P+RFLNWE  + ++W+PFG+GRRA                        ++V E E 
Sbjct: 421 TSFRPERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGE-EP 480

[Close]

BLAST of PI0012741 vs. ExPASy TrEMBL
Match: A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 7.2e-160
Identity = 311/514 (60.51%), Postives = 368/514 (71.60%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDL 60
           ME +T+TC SLSLL +TLI + KLRK ++ +  PP+PPSLP+IGHLHLLK+PFHRVL DL
Sbjct: 12  MEANTITCLSLSLL-LTLILIYKLRK-QSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDL 71

Query: 61  SNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAP 120
           SNK+GPILSL+IGSR ++V+SSPTAVRECFTKNDI  ANRPRLL+GKY+NYNYT V FA 
Sbjct: 72  SNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFAS 131

Query: 121 YGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLK 180
           YG HWRN     TTELLSNHRLN Y+NIRV+ELKL VKN         DFV + M+SKL 
Sbjct: 132 YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLN 191

Query: 181 ELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEG 240
           ELSFN VMRMVSGKRYFGV+VEDVEE  EFREIMKELL+L GA+N ADF  IL++FD++G
Sbjct: 192 ELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQG 251

Query: 241 VKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSD 300
           ++KRM++ASGRAD F+Q LID ER+RR SRW EE  K+G  +MIQ+LLSLQESQP +YSD
Sbjct: 252 LEKRMVQASGRADSFLQNLIDCERDRRASRWPEE--KRGNKTMIQSLLSLQESQPQHYSD 311

Query: 301 DIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD--------------- 360
           DIIKGHVLT                MSLLLNHP +M KAW EIR+               
Sbjct: 312 DIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSS 371

Query: 361 ---------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK----- 420
                     TLRLFPA PLLVPHE SE+CR+EGF++PKGTML+VNAWAIHRDP+     
Sbjct: 372 LKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDP 431

Query: 421 ------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHEQ 436
                 RFLNWE  + ++W+PFG+GRRA                        ++V E E 
Sbjct: 432 TSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGE-EP 491

[Close]

BLAST of PI0012741 vs. ExPASy TrEMBL
Match: A0A0A0L0E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641750 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 5.8e-149
Identity = 273/312 (87.50%), Postives = 292/312 (93.59%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPH +TC SLSLL + TLI LSKLRK+RNQ+KLPPTPPSLPIIGHLHLLK+PF+RVLHD
Sbjct: 1   MEPHALTCLSLSLLLISTLILLSKLRKLRNQRKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR V+VVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTA+GFA
Sbjct: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSG-GDFVVVYMKSK 180
           PYGQHWRN     TTELLSNHRLNTY+NIRVEELKLWVKN+LYKWSSG GDFVVV MK K
Sbjct: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180

Query: 181 LKELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDF 240
           LKELSFNTVMRM+SGKRY+GVEVEDVEE LEFREIMKELLELSGATNAADFFTIL+VFD 
Sbjct: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240

Query: 241 EGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYY 300
           EGVKK+MMKASGRADVF+QKLIDEERE+ VSRW EEKQK+GKTSMI+TLLSLQESQP YY
Sbjct: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300

Query: 301 SDDIIKGHVLTM 306
           SDDIIKGHVL +
Sbjct: 301 SDDIIKGHVLNL 312

[Close]

BLAST of PI0012741 vs. NCBI nr
Match: KAA0064424.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK20163.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 748.4 bits (1931), Expect = 3.4e-212
Identity = 394/515 (76.50%), Postives = 411/515 (79.81%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPHT+T FSLSLL + TLI LSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLH+
Sbjct: 9   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 68

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR VLVVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAVGFA
Sbjct: 69  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 128

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKL 180
           PYGQHWRN     TTELLSNHRLN Y+NIRVEELKLWVKNNLYKW SGGDFVVV MKSKL
Sbjct: 129 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKL 188

Query: 181 KELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFE 240
           KELSFNTVMRM+SGKRYFGVEVEDVEE  EFREIMKELLELSGATNAADF  IL+VFD E
Sbjct: 189 KELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 248

Query: 241 GVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYS 300
           GVKKRMMKASGRADVF+QKLIDEER++RVSRW EEKQKQ KTSMI+TLLSLQESQP YYS
Sbjct: 249 GVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYS 308

Query: 301 DDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD-------------- 360
           DDIIKGHVLT                MSLLLNHPM MKKAWIEIRD              
Sbjct: 309 DDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVS 368

Query: 361 ----------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK---- 420
                      TLR+FPA PLLVPHECSEDC IEGFEVPKGTML+VNAW IHRDPK    
Sbjct: 369 ELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWED 428

Query: 421 -------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHE 436
                  RFLNWEGV+ Y+WIPFGMGRRA                        +KVDE E
Sbjct: 429 PTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQE 488

[Close]

BLAST of PI0012741 vs. NCBI nr
Match: XP_004141393.1 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8649872.1 hypothetical protein Csa_012081 [Cucumis sativus])

HSP 1 Score: 742.7 bits (1916), Expect = 1.9e-210
Identity = 390/516 (75.58%), Postives = 416/516 (80.62%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPH +TC SLSLL + TLI LSKLRK+RNQ+KLPPTPPSLPIIGHLHLLK+PF+RVLHD
Sbjct: 1   MEPHALTCLSLSLLLISTLILLSKLRKLRNQRKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR V+VVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTA+GFA
Sbjct: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSG-GDFVVVYMKSK 180
           PYGQHWRN     TTELLSNHRLNTY+NIRVEELKLWVKN+LYKWSSG GDFVVV MK K
Sbjct: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180

Query: 181 LKELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDF 240
           LKELSFNTVMRM+SGKRY+GVEVEDVEE LEFREIMKELLELSGATNAADFFTIL+VFD 
Sbjct: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240

Query: 241 EGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYY 300
           EGVKK+MMKASGRADVF+QKLIDEERE+ VSRW EEKQK+GKTSMI+TLLSLQESQP YY
Sbjct: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300

Query: 301 SDDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD------------- 360
           SDDIIKGHVLT                MSLLLNHPM+M+KAWIEIRD             
Sbjct: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360

Query: 361 -----------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK--- 420
                       TLR+FPA PLLVPHECSEDC IEGFE+PKGTMLMVNAWAIHRDPK   
Sbjct: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420

Query: 421 --------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEH 436
                   RFLNWEGV+ Y+WIPFGMGRRA                        EKVDE+
Sbjct: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480

[Close]

BLAST of PI0012741 vs. NCBI nr
Match: XP_008453067.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 738.4 bits (1905), Expect = 3.5e-209
Identity = 391/515 (75.92%), Postives = 408/515 (79.22%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFV-TLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHD 60
           MEPHT+T FSLSLL + TLI LSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLH+
Sbjct: 6   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 65

Query: 61  LSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFA 120
           LSNKYGPILSLTIGSR VLVVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAVGFA
Sbjct: 66  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 125

Query: 121 PYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKL 180
           PYGQHWRN     TTELLSNHRLN Y+NIRVEELKLWVKNNLYKW SGGDFVVV MKSKL
Sbjct: 126 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKL 185

Query: 181 KELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFE 240
           KELSFNTVMRM+SGKRYFGVEVEDVEE  EFREIMKELLELSGATNAADF  IL+VFD E
Sbjct: 186 KELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLE 245

Query: 241 GVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYS 300
           GVKKRMMKASGRADVF+QKLIDEER++   RW EEKQKQ KTSMI+TLLSLQESQP YYS
Sbjct: 246 GVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQESQPQYYS 305

Query: 301 DDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD-------------- 360
           DDIIKGHVLT                MSLLLNHPM MKKAWIEIRD              
Sbjct: 306 DDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVS 365

Query: 361 ----------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK---- 420
                      TLR+FPA PLLVPHECSEDC IEGFEVPKGTML+VNAW IHRDPK    
Sbjct: 366 ELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWED 425

Query: 421 -------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHE 436
                  RFLNWEGV+ Y+WIPFGMGRRA                        +KVDE E
Sbjct: 426 PTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQE 485

[Close]

BLAST of PI0012741 vs. NCBI nr
Match: XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 658.3 bits (1697), Expect = 4.6e-185
Identity = 363/518 (70.08%), Postives = 392/518 (75.68%), Query Frame = 0


Query: 1   MEPHTVTC---FSLSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVL 60
           MEPHT+T    F +SL   T I LSKLRK+ N KKLPP+PPSLP+IGHLHLL++P HRVL
Sbjct: 1   MEPHTITWFFPFLISLFLFTFILLSKLRKI-NHKKLPPSPPSLPVIGHLHLLRDPLHRVL 60

Query: 61  HDLSNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
           H+LSNKYGPILSL+IGSR V+VVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG
Sbjct: 61  HNLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120

Query: 121 FAPYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGD-FVVVYMK 180
           FAPYGQHWRN     TTELLSNHRLNTY+NIRV+ELKLW+K NLYK     D FV V MK
Sbjct: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIK-NLYKLICREDNFVGVEMK 180

Query: 181 SKLKELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVF 240
           SKLKELSFN VMRMVSGKRYFGVEV DVEE LEFRE+ KELLELSGATN ADF TIL+VF
Sbjct: 181 SKLKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVF 240

Query: 241 DFEGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPH 300
           DF+G+ KRM+KASGRADVF+QKLIDE+RERR+SRW E+  K GK SMIQTLLSLQESQP 
Sbjct: 241 DFQGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPED--KHGK-SMIQTLLSLQESQPD 300

Query: 301 YYSDDIIKGHVL----------------TMSLLLNHPMMMKKAWIEIRD----------- 360
           YYSDDIIKGHVL                TMSLLLNHP +MKKAWIEIRD           
Sbjct: 301 YYSDDIIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEA 360

Query: 361 -------------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK- 420
                         TLRLFPA PLLVPHE SEDCRIEGFEVPKGTML+VNAWAIHRDPK 
Sbjct: 361 DVSNLRYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKV 420

Query: 421 ----------RFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVD 436
                     RFLNWEGV+ Y+WIPFGMGRRA                        EKVD
Sbjct: 421 WEDPNSFRPERFLNWEGVESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVD 480

[Close]

BLAST of PI0012741 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591.7 bits (1524), Expect = 5.3e-165
Identity = 319/514 (62.06%), Postives = 370/514 (71.98%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDL 60
           ME  T+T  SLSLLF TLI + KLRK ++ +K PP+PPSLP+IGHLHLLK+PFHRVL DL
Sbjct: 20  MEASTITWLSLSLLF-TLILIHKLRK-QSPRKCPPSPPSLPLIGHLHLLKQPFHRVLQDL 79

Query: 61  SNKYGPILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAP 120
           SNK+GPILSL+IGSR ++V+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT V FA 
Sbjct: 80  SNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAFAS 139

Query: 121 YGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLK 180
           YG HWRN     TTELLSNHRLNTY+NIRV+ELKLWVKN         DFV + M+SKL 
Sbjct: 140 YGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFVRLEMQSKLN 199

Query: 181 ELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEG 240
           ELSFNTVMRMVSGKRYFGV+VEDVEE  EFREIMKELL+LSGA+N ADF  IL++FD++G
Sbjct: 200 ELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDYQG 259

Query: 241 VKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSD 300
           ++KRM+KASGRAD+F+Q LID ERERR S W EE  K G  +MIQ+LLSLQESQP YYSD
Sbjct: 260 LEKRMVKASGRADLFLQNLIDCERERRASPWPEE--KHGNKTMIQSLLSLQESQPQYYSD 319

Query: 301 DIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD--------------- 360
           DIIKGHVLT                MSLLLNHP +M KAW EIR+               
Sbjct: 320 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSS 379

Query: 361 ---------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRD------- 420
                     TLRLFP  PLLVPHE SE+CRIEGF++PKGTML+VNAWAIHRD       
Sbjct: 380 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 439

Query: 421 ----PKRFLNWEGVDFYEWIPFGMGRRAL-----------------------EKVDEHEQ 436
               P+R LNWE  + ++W+PFG+GRRA                        ++V E E 
Sbjct: 440 TSFRPERLLNWESAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGE-EP 499

[Close]

BLAST of PI0012741 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 326.2 bits (835), Expect = 3.9e-89
Identity = 202/497 (40.64%), Postives = 285/497 (57.34%), Query Frame = 0


Query: 12  SLLFVTLIFLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHDLSNKY--GPIL 71
           S+LFV L  +  + K++ +  LPP+P  SLP+IGHL LLK P HR    LS      PI 
Sbjct: 9   SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68

Query: 72  SLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRN- 131
           SL +G+R V V SS +   ECFTKND+V ANRP  +  K++ Y+YT +  A YG HWRN 
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 132 ----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSFNTVM 191
               + E+ SNHRLN++++IR +E++  V      +S   +FV V MKS L +L+FN ++
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQ--EFVKVDMKSMLSDLTFNNIL 188

Query: 192 RMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILK-VFDFEGVKKRMMK 251
           RMV+GKRY+G  VED  E    R+++ +++  +GA NA D+  +L+ V D+E    R+ K
Sbjct: 189 RMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDYE---TRVKK 248

Query: 252 ASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKGHV 311
            +GR D F+Q L+DE+RE      A+EK   G T MI  LL+LQESQP Y++D IIKG++
Sbjct: 249 LAGRLDEFLQGLVDEKRE------AKEK---GNT-MIDHLLTLQESQPDYFTDRIIKGNM 308

Query: 312 LTMSL----------------LLNHPMMMKKAWIEIR----------------------- 371
           L + L                +LNHP ++ KA  EI                        
Sbjct: 309 LALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNI 368

Query: 372 -DYTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRF---------- 429
              TLRL+PA P+L+PH  SEDC++ G+++P+GT+L+ N WAIHRDP+ +          
Sbjct: 369 VSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPER 428

[Close]

BLAST of PI0012741 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-81
Identity = 197/497 (39.64%), Postives = 278/497 (55.94%), Query Frame = 0


Query: 11  LSLLFVTLIFLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHDLSNKYG--PI 70
           LS LF+T+     L K   +  LPP+P   LP+IGHLHLLK+P HR  H +S   G  PI
Sbjct: 9   LSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPI 68

Query: 71  LSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRN 130
             L +G+R V V+SS +   ECFTKND+V ANRP ++  K++ YN+T +  A YG HWRN
Sbjct: 69  FHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRN 128

Query: 131 -----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSFNTV 190
                  E+ S+HR++T+ +IR +E++  +  +L + S  G FV V +KS L  L+FN +
Sbjct: 129 LRRIAAVEIFSSHRISTFSSIRKDEIRRLI-THLSRDSLHG-FVEVELKSLLTNLAFNNI 188

Query: 191 MRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFF-TILKVFDFEGVKKRMM 250
           + MV+GKRY+G   ED +E    RE++ E++  +G+ N AD+  +I  V +FE   K + 
Sbjct: 189 IMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWVTNFENQTKIL- 248

Query: 251 KASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKGH 310
               R D  +QKL+DE+R         EK+K G+T +I  LLS QE++P YY+D IIKG 
Sbjct: 249 --GNRLDRVLQKLVDEKR--------AEKEK-GQT-LIDHLLSFQETEPEYYTDVIIKGI 308

Query: 311 VL----------------TMSLLLNHPMMMKKAWIEIRD--------------------- 370
           +L                 MS LLNHP +++KA  EI D                     
Sbjct: 309 ILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQN 368

Query: 371 ---YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRD------PKRF--- 426
               TLRL+PAVPLL+PH  S++C++ G+++P+ T+L+ N WA+HRD      P+RF   
Sbjct: 369 IVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPE 428

[Close]

BLAST of PI0012741 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 299.3 bits (765), Expect = 5.1e-81
Identity = 191/508 (37.60%), Postives = 286/508 (56.30%), Query Frame = 0


Query: 11  LSLLFVTLIFLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHDLSNKYGPILSL 70
           L LLF+ + +     K + +  LPP PPS P +GHLHL+K P HR+L   SN+YGPI SL
Sbjct: 7   LPLLFLVISYKFLYSKTQ-RFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPIFSL 66

Query: 71  TIGSRHVLVVSSPTAVRECFT-KNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRN-- 130
             GSR V+V++SP+  +E FT +NDIV ++RP  L+ KY+ YN+T VG APYG HWRN  
Sbjct: 67  RFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRNLR 126

Query: 131 ---TTELLSNHRLNTYMNIRVEE-LKLWVKNNLYKWSS--GGDFVVVYMKSKLKELSFNT 190
              + E+LS+HRL  + +IR +E L++  + + Y  +S    DF  + ++  L +L+FN 
Sbjct: 127 RICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLTFNN 186

Query: 191 VMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKRMM 250
           ++RMV+GKRY+G +V + EE   F++++ ++   SGA ++AD+  ILK+F  +  K+  +
Sbjct: 187 IVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKLFGNKFEKE--V 246

Query: 251 KASGRA-DVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKG 310
           KA G++ D  +Q+L+DE R          + K+G T M+  L+SLQ+ QP YY+D IIKG
Sbjct: 247 KAIGKSMDDILQRLLDECR----------RDKEGNT-MVNHLISLQQQQPEYYTDVIIKG 306

Query: 311 HVLTMSL----------------LLNHPMMMKKAWIEIRD-------------------- 370
            +++M L                LL +P +++KA  EI +                    
Sbjct: 307 LMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQ 366

Query: 371 ----YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPK---------- 430
                T RLFP  P L+P   ++D +I G++VP+ T++MVNAWAIHRDP+          
Sbjct: 367 NVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNP 426

Query: 431 -RFLNWEGVDFYEW--IPFGMGRRAL-----------------------EKVDEHEQIDL 433
            R+ +  G D+Y +  +PFG GRR                         E V + E++D+
Sbjct: 427 DRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENV-KGEEMDM 486

[Close]

BLAST of PI0012741 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 298.5 bits (763), Expect = 8.7e-81
Identity = 188/500 (37.60%), Postives = 276/500 (55.20%), Query Frame = 0


Query: 9   FSLSLLFVTLIFLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHDLSNKYG-- 68
           F+   + ++LIFL  + +++ +  LPP+P  +LP+IGHL LLK P HRV   +S   G  
Sbjct: 8   FTFCFIVLSLIFL--IGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDA 67

Query: 69  PILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHW 128
           PI+SL +G+R + VVSS +   ECFTKND++ ANR   +S K+I+Y  + V  A Y +HW
Sbjct: 68  PIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHW 127

Query: 129 RN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKSKLKELSFN 188
           RN       E+ S HRLN++ +IR +E++  +   L   S G  F  V MKS   +L+FN
Sbjct: 128 RNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYG--FTKVEMKSMFSDLTFN 187

Query: 189 TVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILKVFDFEGVKKRM 248
            ++RM++GK Y+G   ED  E    R ++ E +  SG  NAAD+  IL    +   + R+
Sbjct: 188 NIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRI 247

Query: 249 MKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPHYYSDDIIKG 308
            K +GR D F+Q L+DE+RE       +EK+   + +M+  LL LQE+QP YY D IIKG
Sbjct: 248 KKLAGRLDEFLQGLVDEKRE------GKEKK---ENTMVDHLLCLQETQPEYYMDRIIKG 307

Query: 309 HVLT----------------MSLLLNHPMMMKKAWIEIR--------------------- 368
            +L+                +S LLN+P ++ KA  EI                      
Sbjct: 308 TMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQ 367

Query: 369 ---DYTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKRFLNWEGVDF 426
                TLRL+PA P+L+PH  S+DC++ G+++P+GTML+ NAWAIHRDP   L W+    
Sbjct: 368 NIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDP---LLWDDPTS 427

[Close]

BLAST of PI0012741 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 298.5 bits (763), Expect = 8.7e-81
Identity = 200/516 (38.76%), Postives = 283/516 (54.84%), Query Frame = 0


Query: 1   MEPHTVTCFSLSLLFVTLIFLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKEPFHRVLHD 60
           M+ + +   S   LF   IFL  L + + +  LPP+P  SLP+IGHLHLLK P HR    
Sbjct: 1   MDLNQILILSFLSLFTLAIFL--LTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLS 60

Query: 61  LSNKYG--PILSLTIGSRHVLVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
           LS   G  P+  L +G+R V V+SS +   ECFTKND+V ANRP+    K++ YN T + 
Sbjct: 61  LSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLL 120

Query: 121 FAPYGQHWRN-----TTELLSNHRLNTYMNIRVEELKLWVKNNLYKWSSGGDFVVVYMKS 180
            A YG HWRN       E+ S HRLN+++ IR +E++  + ++L + S  G FV V MK+
Sbjct: 121 SASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLI-SHLSRDSLHG-FVEVEMKT 180

Query: 181 KLKELSFNTVMRMVSGKRYFGVEVEDVEEGLEFREIMKELLELSGATNAADFFTILK-VF 240
            L  L+ NT +RM++GKRYFG   ED ++    + ++ E +  +GA N  D+ +IL+ V 
Sbjct: 181 LLTNLASNTTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWVS 240

Query: 241 DFEGVKKRMMKASGRADVFVQKLIDEERERRVSRWAEEKQKQGKTSMIQTLLSLQESQPH 300
            +E   KR+     R D F+QKL+DE+R         EK+K G+T MI  LL+LQ+ QP 
Sbjct: 241 SYE---KRIKNLGNRFDTFLQKLVDEKR--------AEKEK-GET-MIDHLLALQDIQPD 300

Query: 301 YYSDDIIKGHVLT----------------MSLLLNHPMMMKKAWIEIRD----------- 360
           YY+D IIKG +LT                MS LLNHP ++KKA +EI +           
Sbjct: 301 YYTDVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDES 360

Query: 361 -------------YTLRLFPAVPLLVPHECSEDCRIEGFEVPKGTMLMVNAWAIHRDPKR 420
                         TLR++PAVPLL+PH  SEDC++ G+++P GTM++ NAWA+HRDP+ 
Sbjct: 361 DIVNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPE- 420

Query: 421 FLNWEGVDFY------------EWIPFGMGRRAL-----------------------EKV 433
              WE  + +            + I FGMGRRA                        E+V
Sbjct: 421 --VWEDPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERV 480

The following BLAST results are available for this feature:

BLAST of PI0012741 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	2.6e-98	42.66	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	1.8e-91	42.66	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
P93147	6.9e-91	39.08	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ9	9.9e-90	41.50	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q6WNR0	3.5e-87	38.87	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of PI0012741 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VB12	1.6e-212	76.50	Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3BWG9	1.7e-209	75.92	LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1F8L9	3.1e-163	61.48	isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IL26	7.2e-160	60.51	isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ...	[more]
A0A0A0L0E6	5.8e-149	87.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641750 PE=3 SV=1	[more]

BLAST of PI0012741 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0064424.1	3.4e-212	76.50	cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK20163.1 cytochrome P450...	[more]
XP_004141393.1	1.9e-210	75.58	cytochrome P450 81Q32 [Cucumis sativus] >KAE8649872.1 hypothetical protein Csa_0...	[more]
XP_008453067.1	3.5e-209	75.92	PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038896686.1	4.6e-185	70.08	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_023535658.1	5.3e-165	62.06	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0012741 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	3.9e-89	40.64	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37320.1	1.0e-81	39.64	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37430.1	5.1e-81	37.60	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT4G37360.1	8.7e-81	37.60	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37330.1	8.7e-81	38.76	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 383..393 score: 44.33 coord: 320..338 score: 26.16 coord: 177..195 score: 19.12 coord: 61..80 score: 35.97 coord: 85..106 score: 26.62
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 326..392 e-value: 2.6E-15 score: 56.2 coord: 34..305 e-value: 5.5E-21 score: 74.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 20..430 e-value: 6.3E-66 score: 224.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 34..425
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 395..431 coord: 26..322
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 324..392
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 395..431 coord: 26..322
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 324..392

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0012741.1	PI0012741.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen