PI0012707 (gene) Melon (PI 482460) v1

BLAST of PI0012707 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.3e-65
Identity = 139/343 (40.52%), Postives = 206/343 (60.06%), Query Frame = 0

Query: 7   FVSNWEGVESVPETYVYPPEKRF--GVSVKKTIPVIDWATDNRALLSKKIFDVSQEFGFF 66
           FVSNW   +SV E+++ P +KR    V V   +P+ID    +  LL ++I    Q+FG F
Sbjct: 4   FVSNW-STKSVSESFIAPLQKRAEKDVPVGNDVPIID-LQQHHHLLVQQITKACQDFGLF 63

Query: 67  QVINHGVSKTLVEETMKIVKEFHGMSPEDKER-----ECSK---DPDKSCKVYSSSQNYE 126
           QVINHG  + L+ ETM++ KEF  +  E+KE+     E +K     ++  K+Y   +   
Sbjct: 64  QVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLS 123

Query: 127 SEQIHLWKDTLKLRCHPNLDKNL-QFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGL 186
           +E+   WKDTL   CHP LD++L   WP+ P K+REVV  Y V + K  + +L+ + EGL
Sbjct: 124 NEEFLYWKDTLAHGCHP-LDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183

Query: 187 GVEKGYFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQ--FLKD 246
           G++ GYF  E+S+    L ++YPPCP+PS T+G   H D  LIT+L QD+ GLQ   +KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243

Query: 247 GQWIDLDPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIE 306
             WI + P+P AF+VNLG  L+VI+N K + + HRVVT+ T  R S+  ++ P+   TIE
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303

Query: 307 PSKCFINEANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFN 337
           P+K  +N+ NPP Y+   + +F D  +S   D ++ +K    N
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDSGVKPYKIN 343

BLAST of PI0012707 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.9e-59
Identity = 125/335 (37.31%), Postives = 196/335 (58.51%), Query Frame = 0

Query: 6   KFVSNWEGVESVPETYVYPPEKR---FGVSVKKTIPVIDWATDNRALLSKKIFDVSQEFG 65
           K +S      ++PE YV P   R     VS  +  P+ID ++ +R+ L ++I      FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 66  FFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHL 125
           FFQVINHGV+K +++E + + +EF  MS E+K +  S DP K+ ++ S+S N + E+++ 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL-STSFNVKKEEVNN 123

Query: 126 WKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYF 185
           W+D L+L C+P + K +  WP NPP F+E+V  Y   + +   +I E++SE LG+EK Y 
Sbjct: 124 WRDYLRLHCYP-IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 183

Query: 186 RGEMSKNPEFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD--VNGLQFLKDGQWIDL 245
           +  + +  + + V++YPPCP P LT GL  H DP  +TIL QD  V GLQ L DGQW  +
Sbjct: 184 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 243

Query: 246 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCF- 305
           +P PDAF++N+G  L+ +SNG  K+  HR VTNT   R S+   + P     + P+K   
Sbjct: 244 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 303

Query: 306 --INEANPPHYRSLEFKDFIDKLMSKPPDRETAMK 332
              ++   P Y+   + ++  K  S+  D+E  ++
Sbjct: 304 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of PI0012707 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.6e-57
Identity = 114/323 (35.29%), Postives = 185/323 (57.28%), Query Frame = 0

Query: 15  ESVPETYVYPPEKRFGVSV---KKTIPVIDWATDNRALLSKKIFDVSQEFGFFQVINHGV 74
           +++P  YV P  +R  + +      IPV+D A+ +RA +   + D  +  GFFQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 75  SKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHLWKDTLKLRC 134
              L+   M++ +EF  +  E+K +  S DP K  ++ S+S N   E +H W+D L+L C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRL-STSFNVRKETVHNWRDYLRLHC 137

Query: 135 HPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYFRGEMSKNPE 194
           +P L + +  WP NPP F+E++  Y   + +    + E +SE LG+E GY R  + +  +
Sbjct: 138 YP-LHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQ 197

Query: 195 FL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD--VNGLQFLKDGQWIDLDPLPDAFLV 254
            + V++YP CP P LT GL  H DP  +TIL  D  V GLQ L DG+WI ++P P A ++
Sbjct: 198 HMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVI 257

Query: 255 NLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINEANPPHYR 314
           N+G  L+ +SNGK ++  HR V N+   R S+   + P + V + P+K  I + +P  YR
Sbjct: 258 NIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYR 317

Query: 315 SLEFKDFIDKLMSKPPDRETAMK 332
           +  + ++  K  S+  D+E  ++
Sbjct: 318 NYTYDEYYKKFWSRNLDQEHCLE 338

BLAST of PI0012707 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 208.8 bits (530), Expect = 9.9e-53
Identity = 109/308 (35.39%), Postives = 179/308 (58.12%), Query Frame = 0

Query: 16  SVPETYVY-----PPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQEFGFFQVINHG 75
           S+PE++V+     PP  +  VS    +P+ID +   R  + + I +  +E GFFQV+NHG
Sbjct: 13  SLPESFVFASDKVPPATKAVVS----LPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHG 72

Query: 76  VSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHLWKDTLKLR 135
           VSK ++ +   + ++F  +   DK    S++  K  +++S +  Y++     W+D L+L 
Sbjct: 73  VSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLFSGA-TYDTGGEKYWRDCLRLA 132

Query: 136 CHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYFRGEMSKNP 195
           C   +D ++  WP  P   R+V++ +         E+L +L EG+G++  YF G++S   
Sbjct: 133 CPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGN 192

Query: 196 EFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD-VNGLQFLKDGQWIDLDPLPDAFLV 255
             L ++HYP CPNP   +G   H D  LIT+L    VNGL+    G WI +DP P+AF+V
Sbjct: 193 VILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVV 252

Query: 256 NLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINEANPPHYR 315
           N G  LEV++NG +K+ EHR +TN+ ++R S+   + P  +  I P+K F+++ NPP YR
Sbjct: 253 NFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYR 312

Query: 316 SLEFKDFI 317
           +  F+DF+
Sbjct: 313 TTMFRDFM 314

BLAST of PI0012707 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 113/325 (34.77%), Postives = 183/325 (56.31%), Query Frame = 0

Query: 8   VSNWEGVESVPETYVYPPEKRFGVSVKK----TIPVIDWAT---DNRALLSKKIFDVSQE 67
           VS++     +P  YV P   R  +S  +    +IP+ID       NRA++ +++      
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 68  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQI 127
           +GFFQ+ NHGV  T V +   + +EF      ++ +  S DP K+ ++ S+S N  ++++
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL-STSFNVGADKV 130

Query: 128 HLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKG 187
             W+D L+L C P ++  ++ WP +P  FREV   Y   +    L +LE +SE LG+E  
Sbjct: 131 LNWRDFLRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESD 190

Query: 188 YFRGEMSKNPEFLV-HHYPPCPNPSLTMGLSQHADPTLITILFQD-VNGLQFLKDGQWID 247
           +    + K+ + +  ++YPPCP P LT GL  H DPT+IT+L QD V+GLQ  KD +W+ 
Sbjct: 191 HISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVA 250

Query: 248 LDPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCF 307
           + P+P+ F+VN+G  ++VISN K K+  HR V NT   R S+    +P +D  I P+   
Sbjct: 251 VSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHEL 310

Query: 308 INEANP-PHYRSLEFKDFIDKLMSK 323
           +NE +    YR+  F ++ DK  ++
Sbjct: 311 VNEQDSLAIYRTYPFVEYWDKFWNR 333

BLAST of PI0012707 vs. ExPASy TrEMBL
Match: A0A1S3BRU2 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.5e-176
Identity = 301/339 (88.79%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMSQTKFVSNW+GVESVPETYVYPPEKRFGVSVKKTIPVIDWA D+R LL KK+ DV+QE
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPD-KSCKVYSSSQNYESEQ 120
           FGF QVINHGV KTLVEETMKI+KEFH MSPEDKERECSKDPD KSC+VYSSSQNYE+EQ
Sbjct: 61  FGFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 IHLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEK 180
           IHLWKDTLKL CHPNLD+N+QFWPQNPPKFREVVKAYL+ +EKFALEILEVLSEGLG+EK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 GYFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDL 240
           GY RGEMSKNPE  VHHYPPCPNPSLT+GL+QHADPTLITILFQDVNGLQFLKDGQWID+
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFI 300
           DPLP+AFLVNLGYVLEVISNGKMKA EHRVVTNTT SRQSL YVVYP +DV IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           NEANPPHY SLEFKDFI KLMSKPPDRETA+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of PI0012707 vs. ExPASy TrEMBL
Match: A0A5A7V1J0 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00760 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.3e-176
Identity = 299/339 (88.20%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMSQTKFVSNW+GVESVPETYVYPPEKRFG+SVKKTIPVIDWA D+RALL KK+ DV++E
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTRE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPD-KSCKVYSSSQNYESEQ 120
           FGF QVINHGV KTLVEETMK++KEFH MSPEDKERECSKDPD KSC+VYSSSQNYE+EQ
Sbjct: 61  FGFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 IHLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEK 180
           IHLWKDTLKL CHPNLD+N+QFWPQNPPKFREVVKAYL+ +EKFALEILEVLSEGLG+EK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 GYFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDL 240
           GY RGEMSKNPE  VHHYPPCPNPSLT+GL+QHADPTLITILFQDVNGLQFLKDGQWID+
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFI 300
           DPLP+AFLVNLGYVLEVISNGKMKA EHRVVTNTT SRQSL YVVYP +DV IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           NEANPPHY SLEFKDFI KLMSKPPDRETA+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of PI0012707 vs. ExPASy TrEMBL
Match: A0A0A0K5H0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.1e-174
Identity = 295/337 (87.54%), Postives = 320/337 (94.96%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMS+TKFVSNW GV SVPETYVYPPEKRFG+SVKK+IPVIDW +D+RALL K++ DV+QE
Sbjct: 1   MMSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDW-SDDRALLLKRVHDVTQE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQI 120
           FGF QVINHGV KTLVEE MKI+KEFHGMS EDKERECSKDPDKSCKVY+SSQNYE+EQI
Sbjct: 61  FGFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQI 120

Query: 121 HLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKG 180
           HLWKDT KLRCHPNLDKNL+FWPQNPPK REVVKAY+VM+EKFA EILEVLSEGLG+EKG
Sbjct: 121 HLWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKG 180

Query: 181 YFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLD 240
           YFRGEMSKNPEFL HHYPPCPNPSLT+GLSQHADPTLITILFQDVNGLQFLKDGQWID+D
Sbjct: 181 YFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVD 240

Query: 241 PLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFIN 300
           PLPDAFLVNLGYV EVISNGK+KA EHRVVTN+T SRQ+LVYVVYPE+DVTIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFIN 300

Query: 301 EANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQ 338
           EANPPHYRSLEFKDF +KLMSKPPDR+TAM+FISF+Q
Sbjct: 301 EANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of PI0012707 vs. ExPASy TrEMBL
Match: A0A0A0K745 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.6e-162
Identity = 273/337 (81.01%), Postives = 309/337 (91.69%), Query Frame = 0

Query: 2   MSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQEF 61
           MSQTKFVSNW+G+ SVPETYV PPEKR GVSVKK IPVID AT++R LLSKK+ DVSQEF
Sbjct: 1   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQEF 60

Query: 62  GFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIH 121
           GFFQVINHGVSK LVEETM++ +EFH MS EDKE ECSKDP+KSC +YSS+ NYE E++H
Sbjct: 61  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 120

Query: 122 LWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGY 181
           LWKD+L+L C+PNL+KN+QFWPQNPPKFREV++AYLV+M+KF+LEILEVLSEGLG+EK Y
Sbjct: 121 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 180

Query: 182 FRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLDP 241
           F G MS NPEFLVHHYPPCPNPSLT+GL++HADPT+ITILFQDVNGLQFLKDG+WID+DP
Sbjct: 181 FSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 240

Query: 242 LPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINE 301
           LPDAFLVNLGYV EVISNGKMKA EHRVVTNTT SRQSL Y VYPE+++TIEPSKCFINE
Sbjct: 241 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 300

Query: 302 ANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           ANPPHYR+LEFKDF++KL SK PDRE AMKFISFNQP
Sbjct: 301 ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 337

BLAST of PI0012707 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 1.1e-123
Identity = 213/335 (63.58%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 6   KFVSNWEGVESVPETYVYPPEKRFG---VSVKKTIPVIDWATDNRALLSKKIFDVSQEFG 65
           KFVSNWE VESVPE+YVYPPEKR G   V + K IPVID +  +R LL +KI D S+EFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 66  FFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHL 125
           FFQ+INHGVSK + EETM+I KEFH MS  +K +ECSKDP++SC+VY+SS+NY  EQ+H 
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 126 WKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYF 185
           W+D L   CHP L+K + FWPQ PPK+REVV AY + M K  LEILE++SEGLG+ KGYF
Sbjct: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186

Query: 186 RGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLDPL 245
            GEMS+NP  LV+HYPPCPNPSLT+GLSQH DP+LITILFQDVNGLQ LKDGQWI + P+
Sbjct: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246

Query: 246 PDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINEA 305
            +AF+VN+G+VL++I+NGK++A EHR VTN+  SRQSL Y+VYP+ +  +EP+KC INEA
Sbjct: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306

Query: 306 NPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQ 338
           NPP YRSL FKDF    + +  D +  M++I  NQ
Sbjct: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

BLAST of PI0012707 vs. NCBI nr
Match: XP_008451257.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 627.9 bits (1618), Expect = 5.2e-176
Identity = 301/339 (88.79%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMSQTKFVSNW+GVESVPETYVYPPEKRFGVSVKKTIPVIDWA D+R LL KK+ DV+QE
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPD-KSCKVYSSSQNYESEQ 120
           FGF QVINHGV KTLVEETMKI+KEFH MSPEDKERECSKDPD KSC+VYSSSQNYE+EQ
Sbjct: 61  FGFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 IHLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEK 180
           IHLWKDTLKL CHPNLD+N+QFWPQNPPKFREVVKAYL+ +EKFALEILEVLSEGLG+EK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 GYFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDL 240
           GY RGEMSKNPE  VHHYPPCPNPSLT+GL+QHADPTLITILFQDVNGLQFLKDGQWID+
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFI 300
           DPLP+AFLVNLGYVLEVISNGKMKA EHRVVTNTT SRQSL YVVYP +DV IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           NEANPPHY SLEFKDFI KLMSKPPDRETA+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of PI0012707 vs. NCBI nr
Match: KAA0059649.1 (hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK08181.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 8.9e-176
Identity = 299/339 (88.20%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMSQTKFVSNW+GVESVPETYVYPPEKRFG+SVKKTIPVIDWA D+RALL KK+ DV++E
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTRE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPD-KSCKVYSSSQNYESEQ 120
           FGF QVINHGV KTLVEETMK++KEFH MSPEDKERECSKDPD KSC+VYSSSQNYE+EQ
Sbjct: 61  FGFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 IHLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEK 180
           IHLWKDTLKL CHPNLD+N+QFWPQNPPKFREVVKAYL+ +EKFALEILEVLSEGLG+EK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 GYFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDL 240
           GY RGEMSKNPE  VHHYPPCPNPSLT+GL+QHADPTLITILFQDVNGLQFLKDGQWID+
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFI 300
           DPLP+AFLVNLGYVLEVISNGKMKA EHRVVTNTT SRQSL YVVYP +DV IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           NEANPPHY SLEFKDFI KLMSKPPDRETA+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of PI0012707 vs. NCBI nr
Match: XP_004148957.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44743.1 hypothetical protein Csa_016242 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 6.3e-174
Identity = 295/337 (87.54%), Postives = 320/337 (94.96%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMS+TKFVSNW GV SVPETYVYPPEKRFG+SVKK+IPVIDW +D+RALL K++ DV+QE
Sbjct: 1   MMSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDW-SDDRALLLKRVHDVTQE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQI 120
           FGF QVINHGV KTLVEE MKI+KEFHGMS EDKERECSKDPDKSCKVY+SSQNYE+EQI
Sbjct: 61  FGFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQI 120

Query: 121 HLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKG 180
           HLWKDT KLRCHPNLDKNL+FWPQNPPK REVVKAY+VM+EKFA EILEVLSEGLG+EKG
Sbjct: 121 HLWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKG 180

Query: 181 YFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLD 240
           YFRGEMSKNPEFL HHYPPCPNPSLT+GLSQHADPTLITILFQDVNGLQFLKDGQWID+D
Sbjct: 181 YFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVD 240

Query: 241 PLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFIN 300
           PLPDAFLVNLGYV EVISNGK+KA EHRVVTN+T SRQ+LVYVVYPE+DVTIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFIN 300

Query: 301 EANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQ 338
           EANPPHYRSLEFKDF +KLMSKPPDR+TAM+FISF+Q
Sbjct: 301 EANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of PI0012707 vs. NCBI nr
Match: XP_011659768.2 (protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KAE8646291.1 hypothetical protein Csa_015678 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 2.5e-162
Identity = 274/338 (81.07%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MMSQTKFVSNWEGVESVPETYVYPPEKRFGVSVKKTIPVIDWATDNRALLSKKIFDVSQE 60
           MMSQTKFVSNW+G+ SVPETYV PPEKR GVSVKK IPVID AT++R LLSKK+ DVSQE
Sbjct: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQE 60

Query: 61  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQI 120
           FGFFQVINHGVSK LVEETM++ +EFH MS EDKE ECSKDP+KSC +YSS+ NYE E++
Sbjct: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 120

Query: 121 HLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKG 180
           HLWKD+L+L C+PNL+KN+QFWPQNPPKFREV++AYLV+M+KF+LEILEVLSEGLG+EK 
Sbjct: 121 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 180

Query: 181 YFRGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLD 240
           YF G MS NPEFLVHHYPPCPNPSLT+GL++HADPT+ITILFQDVNGLQFLKDG+WID+D
Sbjct: 181 YFSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD 240

Query: 241 PLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFIN 300
           PLPDAFLVNLGYV EVISNGKMKA EHRVVTNTT SRQSL Y VYPE+++TIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 300

Query: 301 EANPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQP 339
           EANPPHYR+LEFKDF++KL SK PDRE AMKFISFNQP
Sbjct: 301 EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 338

BLAST of PI0012707 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 453.0 bits (1164), Expect = 2.3e-123
Identity = 213/335 (63.58%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 6   KFVSNWEGVESVPETYVYPPEKRFG---VSVKKTIPVIDWATDNRALLSKKIFDVSQEFG 65
           KFVSNWE VESVPE+YVYPPEKR G   V + K IPVID +  +R LL +KI D S+EFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 66  FFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHL 125
           FFQ+INHGVSK + EETM+I KEFH MS  +K +ECSKDP++SC+VY+SS+NY  EQ+H 
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 126 WKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYF 185
           W+D L   CHP L+K + FWPQ PPK+REVV AY + M K  LEILE++SEGLG+ KGYF
Sbjct: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186

Query: 186 RGEMSKNPEFLVHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLKDGQWIDLDPL 245
            GEMS+NP  LV+HYPPCPNPSLT+GLSQH DP+LITILFQDVNGLQ LKDGQWI + P+
Sbjct: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246

Query: 246 PDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINEA 305
            +AF+VN+G+VL++I+NGK++A EHR VTN+  SRQSL Y+VYP+ +  +EP+KC INEA
Sbjct: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306

Query: 306 NPPHYRSLEFKDFIDKLMSKPPDRETAMKFISFNQ 338
           NPP YRSL FKDF    + +  D +  M++I  NQ
Sbjct: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

BLAST of PI0012707 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.3e-60
Identity = 125/335 (37.31%), Postives = 196/335 (58.51%), Query Frame = 0

Query: 6   KFVSNWEGVESVPETYVYPPEKR---FGVSVKKTIPVIDWATDNRALLSKKIFDVSQEFG 65
           K +S      ++PE YV P   R     VS  +  P+ID ++ +R+ L ++I      FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 66  FFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHL 125
           FFQVINHGV+K +++E + + +EF  MS E+K +  S DP K+ ++ S+S N + E+++ 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL-STSFNVKKEEVNN 123

Query: 126 WKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYF 185
           W+D L+L C+P + K +  WP NPP F+E+V  Y   + +   +I E++SE LG+EK Y 
Sbjct: 124 WRDYLRLHCYP-IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 183

Query: 186 RGEMSKNPEFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD--VNGLQFLKDGQWIDL 245
           +  + +  + + V++YPPCP P LT GL  H DP  +TIL QD  V GLQ L DGQW  +
Sbjct: 184 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 243

Query: 246 DPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCF- 305
           +P PDAF++N+G  L+ +SNG  K+  HR VTNT   R S+   + P     + P+K   
Sbjct: 244 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 303

Query: 306 --INEANPPHYRSLEFKDFIDKLMSKPPDRETAMK 332
              ++   P Y+   + ++  K  S+  D+E  ++
Sbjct: 304 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of PI0012707 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 113/325 (34.77%), Postives = 183/325 (56.31%), Query Frame = 0

Query: 8   VSNWEGVESVPETYVYPPEKRFGVSVKK----TIPVIDWAT---DNRALLSKKIFDVSQE 67
           VS++     +P  YV P   R  +S  +    +IP+ID       NRA++ +++      
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 68  FGFFQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQI 127
           +GFFQ+ NHGV  T V +   + +EF      ++ +  S DP K+ ++ S+S N  ++++
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL-STSFNVGADKV 130

Query: 128 HLWKDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKG 187
             W+D L+L C P ++  ++ WP +P  FREV   Y   +    L +LE +SE LG+E  
Sbjct: 131 LNWRDFLRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESD 190

Query: 188 YFRGEMSKNPEFLV-HHYPPCPNPSLTMGLSQHADPTLITILFQD-VNGLQFLKDGQWID 247
           +    + K+ + +  ++YPPCP P LT GL  H DPT+IT+L QD V+GLQ  KD +W+ 
Sbjct: 191 HISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVA 250

Query: 248 LDPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCF 307
           + P+P+ F+VN+G  ++VISN K K+  HR V NT   R S+    +P +D  I P+   
Sbjct: 251 VSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHEL 310

Query: 308 INEANP-PHYRSLEFKDFIDKLMSK 323
           +NE +    YR+  F ++ DK  ++
Sbjct: 311 VNEQDSLAIYRTYPFVEYWDKFWNR 333

BLAST of PI0012707 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.6e-52
Identity = 117/328 (35.67%), Postives = 178/328 (54.27%), Query Frame = 0

Query: 14  VESVPETYVYPPEKRFGVSVKKT----IPVIDWAT---DNRALLSKKIFDVSQEFGFFQV 73
           V+ VP  YV P   R  +S  +T    IP+ID       NRA +  +        GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 74  INHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHLWKDT 133
            NHGV +  +++ M   +EF   S  ++ +  S D  K+ ++ S+S N   E++  W+D 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRL-STSFNVSKEKVSNWRDF 134

Query: 134 LKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYFRGEM 193
           L+L C+P ++  +  WP  P  FREV   Y   +    L +LE +SE LG+ K      +
Sbjct: 135 LRLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTI 194

Query: 194 SKNPEFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD-VNGLQFLKDGQWIDLDPLPD 253
            K+ + + +++YP CP P LT GL  H D  LIT+L QD V+GLQ  KDG+WI ++P+P+
Sbjct: 195 GKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPN 254

Query: 254 AFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINEA-- 313
            F+VNLG  ++VISN K K+  HR V N+ M R S+     P  D  I P++  INE   
Sbjct: 255 TFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEED 314

Query: 314 NPPHYRSLEFKDFIDKLMSKPPDRETAM 331
           +P  YR+  + ++ +K      D E+ +
Sbjct: 315 SPAIYRNFTYAEYFEKFWDTAFDTESCI 340

BLAST of PI0012707 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.6e-45
Identity = 106/320 (33.12%), Postives = 173/320 (54.06%), Query Frame = 0

Query: 13  GVESVPETYVYPPEKR--FGVSV---KKTIPVIDWATDNRALL-SKKIFDVS---QEFGF 72
           GV  VP+ YV PP +R   G S+   + T+PVID +  ++  L S  I ++S   +EFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 73  FQVINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHLW 132
           FQVINHG+  ++V + +    +F  +  E+K    S +  +  + Y +S N+ ++++H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 133 KDTLKLRCHPNLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLSEGLGVEKGYFR 192
           +D +K   HP L K +  WP NPP +++ V  Y         +++E +SE LG+EK Y +
Sbjct: 143 RDFIKHYSHP-LSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQ 202

Query: 193 GEMSKNPEFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQDVNGLQFLK-DGQWIDLDP 252
            E+ +  + + V+ YP CP P + +G+  H+D + +TIL Q   GLQ +  +  W+ +  
Sbjct: 203 EEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPY 262

Query: 253 LPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPESDVTIEPSKCFINE 312
           +  A +V LG  +EV+SNG  K+  HRV  N  + R S   +        I P+   +N 
Sbjct: 263 IEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNP 322

Query: 313 ANPPHYRSLEFKDFIDKLMS 322
            N P Y    F DF++ + S
Sbjct: 323 NNAPAYGEFSFNDFLNYISS 340

BLAST of PI0012707 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-42
Identity = 106/327 (32.42%), Postives = 166/327 (50.76%), Query Frame = 0

Query: 19  ETYVYPPEKRFGVSVKKT--------IPVIDWAT-----DNRALLSKKIFDVSQEFGFFQ 78
           E ++  PE R    +  +        IP ID ++      ++  ++K+I +  + +GFFQ
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 79  VINHGVSKTLVEETMKIVKEFHGMSPEDKERECSKDPDKSCKVYSSSQNYESEQIHLWK- 138
           VINHG+   L     K   EF  ++ E+K R+  +D       +       ++ +  WK 
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEK-RKVKRDEVNPMGYHDEE---HTKNVRDWKE 125

Query: 139 -------DTLKLRCHP-----NLDKNLQFWPQNPPKFREVVKAYLVMMEKFALEILEVLS 198
                  D+  +   P      L K    WPQNP  FREV + Y   +EK A  +LE++S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185

Query: 199 EGLGVEKGYFRGEMSKNPEFL-VHHYPPCPNPSLTMGLSQHADPTLITILFQD-VNGLQF 258
             LG+      G  ++   FL  +HYPPCPNP L +G+ +H D   +T+L QD V GLQ 
Sbjct: 186 ISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQV 245

Query: 259 LK--DGQWIDLDPLPDAFLVNLGYVLEVISNGKMKATEHRVVTNTTMSRQSLVYVVYPES 316
            +  DGQWI + P+ DA ++N+G  ++V +N +  + EHRVV NT+  R S+ +  +P  
Sbjct: 246 SRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSH 305