PI0012683 (gene) Melon (PI 482460) v1

Overview
NamePI0012683
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionUPF0481 protein At3g47200-like
Locationchr03: 4257435 .. 4260420 (-)
RNA-Seq ExpressionPI0012683
SyntenyPI0012683
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCTTTGCTCCTTTTTATTTATTGAGTTCCACTTTCACCAACCCATCTCTCTTGGTTACTCCATCTTAATTCTTTCCCACATTTCTCTCTTTCTTTCTTCCCTCATTTCATCTCAAATTTTGAACACAAAGGTAGAACCCAAAGGTACTTGTTTTTTATTTTATTTTGTTTTATTAATTTCTTCCCTTAATTCATTTAACTTTCAAGTTTTTTCTATATATGTATCTAGATTAAATATTCTTTTACTCCATTTTCTTCCTTTTGATTCCTCTACTTTAAAAATGTCCATTTTCCTTTGCATACTTTTAACTTTTATTTATTTTAGTCCCACTAGTTCCAAAGGTCCATTTTGTGTAAAAGCCAATAATCATTAGATAGTGTAAAATAAAAATACGTTATATAGAAATGGACTTGTTTGGTAATAACATTCTCATTTCCCGTTTGTTATTTTCTATTATTTTTTTAGAAATTGACTTGTTTGATAACTGTTCTCGTTTCTTGTTTCTAAATTTTGAAAAGTTTCTGAAAGTGGGGTCAAATCTAAAGATTTTTTTTTTTTTTTTTTTTGTGTATGGTAGATAAATGGGGAACACATAAGATAGGTGCATCATGGATGTACCCAATCTTTCACTTTCTTTTTTTTTTAAGTTGTTTCTTTGACATATTTTTAATTCTTTTCTTTATTAATTTTTTTATATTTAATTTGAATTTAAATTATCTATATTTAGATTTATATATTATTTTTATATCCAAAATTTTAATATGTATATTTAAGGGCTATAATCGAATATAACAAAGTGAAACATAATATTTACAAACATGACAAAATTTTACTGTCTATTAATGATAGATGATACATCAATGTCTATTAAGTGATAAGTCAAACATCATTTAAGACAAAATAAGTTAAGTGAGAAATAATATAATGTCAACATCGTTTAAGACAAAATAAGTTGATTGAGAAATAATATAATTTTTTTTTTCATTTCTTGTTGAATTCTCTTAGTTAATTTAATTTTCTGTATAAAATTTTGAATTTAATTGGATGTTAATTACTACTTCAATAAGTTAAACTATTTATAATAACATACAAACAAGTGGGTGCCGATAAATGTAAAATTTTAAATAACTAAAAGTTGTTGATGTAATTATAACAAACCACAAAGTTAATTGGAAAGTTTGATATAATTTAGGGGTTTGTAATTGTAACAAACTCCAATTGATTGCTGCACTGCTAATTTTTAGTTTGAAATCATTACAACGTTTTTATAATAGGGGTGATAACAAAATAATTAAAGAAATACATAGTTTTTAATTTGCTAGTGGTAAAACATTAAGCTTGAAAGCTGATCACCAATAGGTAACGTAACGGCCTATTGATCTATGTGCAGGGTGTTTGTTGAAGGAAATTTAGAAACGTGGGCGTCTTTCAATATTTCAATAAATGGGAAATAGTGAGATTGAACAAGCAAACGATATACCTTACAAACCGGAAGAAACAATAATTAGCGATGCTCATGAAATGCTTCGTGGTAATGTTGTGATACGTATTGAAGAAATTTTAAAACAATTGCCTGAAGTTAATCCAAAAAGCAGCATCTATCGAATTTCCAAAGAGCTACACGAGTTGAATGATAACGCATATACTCCTCAATTCATTTCCATAGGCCCTTTTCACTATGACACTCGAGGGGATTTGCTAGCCAATGAACATTATAAGGTTCAAGGCTTTATTAGCTTTTTACAGCGTATAGATATTAATAAGAAAGAAAACCAGGGTAAAATTACCACATCATTACTGATTTTCCTTGTGGAAAAAAGTCATGGTTGGGTGAAAGAAGCTTATAATTTCTATGCAGAACCAATAAAGATGAAGGAGGAGGAGTTTGTTGTAATGATGCTTGTGGATGCTTGTTTCATAGTCGAGTTTTTTTTACTATTTTGTTATGGTAGATTCCGAGAAGATGGTAAGTTATTCAAAATAGATATTACATTATACTATGGAGCATTCGACAAAATATTCATTGACTTGATAAAGTTGGAAAATCAAGTTCCTTTTATTCTTCTTAAAAATCTATTTAACCTGATACCAAACATGATTCCCACTTCGTTATAGGTGATGAAGAAGAAGATAGCTCTTTCCCCTCGATGATTAATCTTACAATCGAGGCTTTTAAGTGGTTTGGGTGGGGGTCTGACTTTGAGGAATTGTTGTATGAGAAAGAACCAAAGCACTTGGTTGATTTCTTAAGTTTCTTTTTCGAGCCGCCCCCTGATTATGGGCAAGACAAGCAAGAAGGTGAGAACAACAATAATAATTTCTTAAACTTCTTTGGTGACTTGCTGCAGCTGTGTTGTTTTTGGAATAAGAAGCATTGTCCCGAGGAAATGATAAGAGGGAGTCAGTCTCCTCCATCCATAACTAAACTCTGCGAGGCTGGTGTCACCATCACACCAGGAGATATGAACGCAGGACTATGTTTGATTGACATAAACTTCAAAAATGGGGTTTTGAAAATCCCACGTATATGTATTCAAAGTAGAGTCGAAGTCATGATACGAAACGTGATAGCATTTGATCATTACCCAGCAGGAAATGATAAGAGGTCTGCACTCCAATATGTGTCATTTCTGGAAAAATTGATAGAGACAGAGCAAGATGTGCATTTACTTGTGAAGGTTGGAGTCATAATCAACCTTGTTGATGGCAGTGATAAATACATTTTAGAATGGTTTAAAAATCTCTCTAAATATACCTTCAACCAACCTTGGTTCCACTTTTTGAAAATCGGTGAAGCTTTACGTGAACATTGCAATAGACGGAGGAACAGGACAAAAAGTTATTGGAGCAAGGCGAAAGTTACATTAAAACATGACTATTTCAATACGCCATGGGCTGCTATCTCTGTCATTGCTGCAAGTTTCCTCATTATTCTCACCCTCCTTCAAACCATATTCTCTGCTATATCTGCGTTTCCTGGCTAG

mRNA sequence

CAGCTTTGCTCCTTTTTATTTATTGAGTTCCACTTTCACCAACCCATCTCTCTTGGTTACTCCATCTTAATTCTTTCCCACATTTCTCTCTTTCTTTCTTCCCTCATTTCATCTCAAATTTTGAACACAAAGGTAGAACCCAAAGGGTGTTTGTTGAAGGAAATTTAGAAACGTGGGCGTCTTTCAATATTTCAATAAATGGGAAATAGTGAGATTGAACAAGCAAACGATATACCTTACAAACCGGAAGAAACAATAATTAGCGATGCTCATGAAATGCTTCGTGGTAATGTTGTGATACGTATTGAAGAAATTTTAAAACAATTGCCTGAAGTTAATCCAAAAAGCAGCATCTATCGAATTTCCAAAGAGCTACACGAGTTGAATGATAACGCATATACTCCTCAATTCATTTCCATAGGCCCTTTTCACTATGACACTCGAGGGGATTTGCTAGCCAATGAACATTATAAGGTTCAAGGCTTTATTAGCTTTTTACAGCGTATAGATATTAATAAGAAAGAAAACCAGGGTAAAATTACCACATCATTACTGATTTTCCTTGTGGAAAAAAGTCATGGTTGGGTGAAAGAAGCTTATAATTTCTATGCAGAACCAATAAAGATGAAGGAGGAGGAGTTTGTTGTAATGATGCTTGTGGATGCTTGTTTCATAGTCGAGTTTTTTTTACTATTTTGTTATGGTAGATTCCGAGAAGATGGTGATGAAGAAGAAGATAGCTCTTTCCCCTCGATGATTAATCTTACAATCGAGGCTTTTAAGTGGTTTGGGTGGGGGTCTGACTTTGAGGAATTGTTGTATGAGAAAGAACCAAAGCACTTGGTTGATTTCTTAAGTTTCTTTTTCGAGCCGCCCCCTGATTATGGGCAAGACAAGCAAGAAGGTGAGAACAACAATAATAATTTCTTAAACTTCTTTGGTGACTTGCTGCAGCTGTGTTGTTTTTGGAATAAGAAGCATTGTCCCGAGGAAATGATAAGAGGGAGTCAGTCTCCTCCATCCATAACTAAACTCTGCGAGGCTGGTGTCACCATCACACCAGGAGATATGAACGCAGGACTATGTTTGATTGACATAAACTTCAAAAATGGGGTTTTGAAAATCCCACGTATATGTATTCAAAGTAGAGTCGAAGTCATGATACGAAACGTGATAGCATTTGATCATTACCCAGCAGGAAATGATAAGAGGTCTGCACTCCAATATGTGTCATTTCTGGAAAAATTGATAGAGACAGAGCAAGATGTGCATTTACTTGTGAAGGTTGGAGTCATAATCAACCTTGTTGATGGCAGTGATAAATACATTTTAGAATGGTTTAAAAATCTCTCTAAATATACCTTCAACCAACCTTGGTTCCACTTTTTGAAAATCGGTGAAGCTTTACGTGAACATTGCAATAGACGGAGGAACAGGACAAAAAGTTATTGGAGCAAGGCGAAAGTTACATTAAAACATGACTATTTCAATACGCCATGGGCTGCTATCTCTGTCATTGCTGCAAGTTTCCTCATTATTCTCACCCTCCTTCAAACCATATTCTCTGCTATATCTGCGTTTCCTGGCTAG

Coding sequence (CDS)

ATGGGAAATAGTGAGATTGAACAAGCAAACGATATACCTTACAAACCGGAAGAAACAATAATTAGCGATGCTCATGAAATGCTTCGTGGTAATGTTGTGATACGTATTGAAGAAATTTTAAAACAATTGCCTGAAGTTAATCCAAAAAGCAGCATCTATCGAATTTCCAAAGAGCTACACGAGTTGAATGATAACGCATATACTCCTCAATTCATTTCCATAGGCCCTTTTCACTATGACACTCGAGGGGATTTGCTAGCCAATGAACATTATAAGGTTCAAGGCTTTATTAGCTTTTTACAGCGTATAGATATTAATAAGAAAGAAAACCAGGGTAAAATTACCACATCATTACTGATTTTCCTTGTGGAAAAAAGTCATGGTTGGGTGAAAGAAGCTTATAATTTCTATGCAGAACCAATAAAGATGAAGGAGGAGGAGTTTGTTGTAATGATGCTTGTGGATGCTTGTTTCATAGTCGAGTTTTTTTTACTATTTTGTTATGGTAGATTCCGAGAAGATGGTGATGAAGAAGAAGATAGCTCTTTCCCCTCGATGATTAATCTTACAATCGAGGCTTTTAAGTGGTTTGGGTGGGGGTCTGACTTTGAGGAATTGTTGTATGAGAAAGAACCAAAGCACTTGGTTGATTTCTTAAGTTTCTTTTTCGAGCCGCCCCCTGATTATGGGCAAGACAAGCAAGAAGGTGAGAACAACAATAATAATTTCTTAAACTTCTTTGGTGACTTGCTGCAGCTGTGTTGTTTTTGGAATAAGAAGCATTGTCCCGAGGAAATGATAAGAGGGAGTCAGTCTCCTCCATCCATAACTAAACTCTGCGAGGCTGGTGTCACCATCACACCAGGAGATATGAACGCAGGACTATGTTTGATTGACATAAACTTCAAAAATGGGGTTTTGAAAATCCCACGTATATGTATTCAAAGTAGAGTCGAAGTCATGATACGAAACGTGATAGCATTTGATCATTACCCAGCAGGAAATGATAAGAGGTCTGCACTCCAATATGTGTCATTTCTGGAAAAATTGATAGAGACAGAGCAAGATGTGCATTTACTTGTGAAGGTTGGAGTCATAATCAACCTTGTTGATGGCAGTGATAAATACATTTTAGAATGGTTTAAAAATCTCTCTAAATATACCTTCAACCAACCTTGGTTCCACTTTTTGAAAATCGGTGAAGCTTTACGTGAACATTGCAATAGACGGAGGAACAGGACAAAAAGTTATTGGAGCAAGGCGAAAGTTACATTAAAACATGACTATTTCAATACGCCATGGGCTGCTATCTCTGTCATTGCTGCAAGTTTCCTCATTATTCTCACCCTCCTTCAAACCATATTCTCTGCTATATCTGCGTTTCCTGGCTAG

Protein sequence

MGNSEIEQANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSSIYRISKELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQGKITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGDEEEDSSFPSMINLTIEAFKWFGWGSDFEELLYEKEPKHLVDFLSFFFEPPPDYGQDKQEGENNNNNFLNFFGDLLQLCCFWNKKHCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAAISVIAASFLIILTLLQTIFSAISAFPG
Homology
BLAST of PI0012683 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 3.3e-14
Identity = 95/443 (21.44%), Postives = 177/443 (39.95%), Query Frame = 0

Query: 52  IYRISKELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQ 111
           I+R+ +    LN  AY P+ +SIGP+HY  +   +  +H K +    FL   +  KK+ +
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQH-KPRLLQLFLD--EAKKKDVE 107

Query: 112 GKITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYG-R 171
             +    ++ L +K           Y+E +K    + + MM++D CFI+  FL+      
Sbjct: 108 ENVLVKAVVDLEDKIR-------KSYSEELK-TGHDLMFMMVLDGCFILMVFLIMSGNIE 167

Query: 172 FRED---------GDEEED-----SSFPSMINLTIEAFKWFGWGSDFEELLYEKEPKHLV 231
             ED            + D     +  P  +  T+      G  SD   + +        
Sbjct: 168 LSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFH------- 227

Query: 232 DFLSFFFEPPPDYGQDKQEGENNNNNFLNFFGDLLQLCCFWN--------KKHCPEEMIR 291
                FF+ P D  ++    E + N       DL++     N          H   ++  
Sbjct: 228 -----FFKNPID--KEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHE 287

Query: 292 G-SQSPPSITKLCEAGVTITPGDMNAGL----------CLIDINFKNGVLKIPRICIQSR 351
           G S + PS+       +         G+           ++++  K   L+IP++     
Sbjct: 288 GKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGF 347

Query: 352 VEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYI 411
           +     N +AF+ +   +       Y+ F+  L+  E+DV  L    +II    GS+  +
Sbjct: 348 ISSFFLNCVAFEQFYT-DSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEV 407

Query: 412 LEWFKNLSK-YTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAA 460
            E+FK +SK   F     +   + + + E+       TK +++      +H +F +PW  
Sbjct: 408 SEFFKTISKDVVFEVDTSYLNNVFKGVNEY-------TKKWYNGLWAGFRHTHFESPWTF 457

BLAST of PI0012683 vs. ExPASy TrEMBL
Match: A0A5A7VD32 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002550 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 7.6e-115
Identity = 256/510 (50.20%), Postives = 311/510 (60.98%), Query Frame = 0

Query: 6   IEQANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSSIYRISKELHELNDN 65
           I+  +DI YK EE I  DA + L  NVV+ I+ IL+Q+P+VNPK  IYRISKE+ ELND 
Sbjct: 2   IQANHDITYKVEEPITGDADQELCVNVVMFIKNILEQVPQVNPKICIYRISKEVRELNDR 61

Query: 66  AYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKE------NQGKITTSLL 125
           AY PQFISIGPFHY TR DL+ANE+YK QGF +FL+RI IN ++      +Q KI T  +
Sbjct: 62  AYAPQFISIGPFHYHTRQDLIANEYYKYQGFNNFLRRISINNEQIESMEGSQVKINT--V 121

Query: 126 IFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLL-------------- 185
            FLVEK HG +KEA+N YA+ I M EEEFV MMLVDACFIVEF +L              
Sbjct: 122 KFLVEKCHGLMKEAWNCYADQINMMEEEFVGMMLVDACFIVEFLILLRGRVSNIQRIISK 181

Query: 186 ----FCYGRFRED-GD----EEEDSSF----------------------PSMINLTIEAF 245
               F  G   E  GD    E +   F                       S+++LT  AF
Sbjct: 182 IDSKFYQGALYEILGDLIKLENQVPFFLLQSLFDLITKDDLPLVGTHERASLMDLTCFAF 241

Query: 246 KWFGWGSDFEELLYEK-EPKHLVDFLSFFFEPPPDYGQDKQEGENNNNNFLNFFGDLLQL 305
           K F         +Y+K  PKHLVDF S FF  PP Y +D  +  +  + +          
Sbjct: 242 KIFVVAGYHFNYMYDKTTPKHLVDFSSIFFMSPPTYVEDNHKYVHTKDRW---------- 301

Query: 306 CCFWNKKHCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRIC 365
            C                  PS+T L EAGVTI P  + +  CL  I+F+NGVL IP I 
Sbjct: 302 -CI----------------SPSVTTLWEAGVTIQPSYVES--CLTSISFENGVLNIPHIN 361

Query: 366 IQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGS 425
           +    E+MIRN+IAFDHYPAGN    A++YVSFL  L++ EQDVHLLVKVGVI+N V G 
Sbjct: 362 MGKTFEIMIRNLIAFDHYPAGNKNMYAVEYVSFLHDLMKEEQDVHLLVKVGVIVNSVGGG 421

Query: 426 DKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTP 464
           DKYIL++F NLS+Y    P+  F +I E L  +CNRRRNR K YW+KAKVTL+HDYF TP
Sbjct: 422 DKYILDFFNNLSRYNMVSPFSQFGEIAETLHVYCNRRRNRAKRYWNKAKVTLRHDYFTTP 480

BLAST of PI0012683 vs. ExPASy TrEMBL
Match: A0A5A7VGS6 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002590 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 5.6e-110
Identity = 251/524 (47.90%), Postives = 327/524 (62.40%), Query Frame = 0

Query: 1   MGNSEIE--QANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSS-IYRISK 60
           M NSEIE  + N+I ++  E I  D  + +R  VVI I+++L  L  VN K + IY++ K
Sbjct: 1   MKNSEIEMIEDNNISHRVGEIISEDIDQKVRDIVVISIDKMLDGLSPVNSKCNIIYQVPK 60

Query: 61  ELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKK------ENQ 120
           EL E+ND AY PQFISIGPFHY TR DL+ANEHYK+QGF +FL+ I+IN+K      E+Q
Sbjct: 61  ELREINDKAYVPQFISIGPFHYRTRKDLIANEHYKLQGFYNFLRGININEKQVESLEEDQ 120

Query: 121 GKITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRF 180
            KI++ +   LVEKSH W+KEA+N YAEPIKM+EEEF++MMLVDACFIVEFFLL CYG F
Sbjct: 121 VKISSKV---LVEKSHSWMKEAWNCYAEPIKMEEEEFIIMMLVDACFIVEFFLL-CYGSF 180

Query: 181 REDG-----------------------------------------DEEEDSSFP------ 240
           +E                                           D ++ SS        
Sbjct: 181 QEGDRLFKFSLFYYGVFYEILLDLIKLENQVPFFLLQNLFDLIPKDNDDTSSIVDYEAGP 240

Query: 241 -SMINLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEPPP--DYGQDKQEGENNN 300
            S+I+LT  A K FG+  ++E   LY K P+H++D LSF+F P P  D   +KQ  +  N
Sbjct: 241 ISLIDLTYMALKAFGFVREYEINNLYHKNPEHILDLLSFYFLPVPHNDPDMNKQHDKRKN 300

Query: 301 N-NFLNFFGDLLQLCCFWNKKHCPEEMIRGSQ-SPPSITKLCEAGVTITPGDMNAGLCLI 360
             +FL+FF  L      WNKK   +++ +  + SPP+IT+L EAGVTI   +    LC +
Sbjct: 301 KADFLSFFRHL------WNKKSDHKKISKQRRLSPPTITELFEAGVTIKAAE-KENLCFM 360

Query: 361 DINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVH 420
           +I+FKNGVL+IP I I+   EV+IRNV+AF+ +PAGN K+ A+QYV+FL+ LI TE+D+ 
Sbjct: 361 NISFKNGVLEIPPIVIEGTFEVVIRNVLAFEIFPAGNQKKYAIQYVTFLDDLISTEKDLC 420

Query: 421 LLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYW 463
           LLVK GVI N   GSDK   E F +L+K        +F    +ALR HC+         W
Sbjct: 421 LLVKAGVITNDTGGSDKEASELFNSLTKLVTTPLSSYFDDTSKALRVHCD-------GSW 480

BLAST of PI0012683 vs. ExPASy TrEMBL
Match: A0A1S4DW65 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488289 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 5.2e-108
Identity = 255/540 (47.22%), Postives = 308/540 (57.04%), Query Frame = 0

Query: 1   MGNSEIEQA---NDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSS-IYRIS 60
           M NSEI +    NDI        IS+  E +  NVVI I++IL  LP +NPK   IY++S
Sbjct: 1   MENSEIIETKVENDICDDELGETISEI-EKVCDNVVISIDKILGGLPRINPKCHIIYQVS 60

Query: 61  KELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQGKITT 120
           KEL E+ND AY PQFISIGPFH+ TR DL+ANEHYK+QGF +FL RI+     N  +I +
Sbjct: 61  KELREMNDKAYAPQFISIGPFHHRTRNDLIANEHYKLQGFNNFLHRIN-----NYEQIES 120

Query: 121 SLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGD 180
           S     V+K HGWVKEA+N YAEPI M EEEFV+MMLVDACFI+EFF+L     +  D  
Sbjct: 121 SK--EFVKKCHGWVKEAWNCYAEPINMNEEEFVLMMLVDACFILEFFILLIDDHYGGDYL 180

Query: 181 EEEDS-------------------------------------------------SFPSMI 240
            E D                                                   FPS+I
Sbjct: 181 FEADQIFQIQDMVDFSFYRGVFFEILIDLIKLENQVPFFLLQNLFDLMPKHDVPMFPSLI 240

Query: 241 NLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEP--PPD-----YGQDKQEGENN 300
           ++T E   WFG+   ++   LY K+PKHL+DFLSF+F P  P D        +++  + N
Sbjct: 241 DITSEILTWFGFVGKYKINDLYHKKPKHLLDFLSFYFFPLLPNDDHIRFKQNERKNSDQN 300

Query: 301 NNNFLNFFGDLL---------------QLCCFWNKKHCPEEMIRGSQ---SPPSITKLCE 360
           NNN L FF  L                 LCCF NK+    E    +    SPPSIT+LCE
Sbjct: 301 NNNLLRFFRPLFPAHWLKKNNDSFGVPSLCCFSNKEAETREKDSENYFRLSPPSITELCE 360

Query: 361 AGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSAL 420
           AGVTI        LC ++I FKNGVL+IP I I    EV+IRNVIAFD YPAGN+K  A+
Sbjct: 361 AGVTIKAA-KREDLCFMNIGFKNGVLEIPCIDIDCTFEVVIRNVIAFDQYPAGNEKMYAI 420

Query: 421 QYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGE 462
            YV FL+ LI TEQD HLL K G+IIN   GSDK I E F   SK+       HF  I +
Sbjct: 421 HYVLFLDDLINTEQDAHLLTKAGIIINTFGGSDKDITEMFNRFSKFVTFPICSHFDDINK 480

BLAST of PI0012683 vs. ExPASy TrEMBL
Match: A0A0A0LQ57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380610 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 2.9e-106
Identity = 244/522 (46.74%), Postives = 312/522 (59.77%), Query Frame = 0

Query: 1   MGNSEIEQANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPK-SSIYRISKEL 60
           M NSEIE  ++           +  + +R NVVI I+++L+ LP VNPK ++IY++ KEL
Sbjct: 1   MENSEIEIMSE-----------NIDQNVRDNVVISIDKMLEGLPRVNPKCNTIYQVPKEL 60

Query: 61  HELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINK------KENQGK 120
            E+ND AY PQFISIGPFHY TR DL+ANEHYK+QGF +FL RI I        +ENQ  
Sbjct: 61  REINDKAYVPQFISIGPFHYRTRKDLIANEHYKLQGFFNFLGRISIINSHIQLLEENQVN 120

Query: 121 ITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFRE 180
           I++ +   LVEKSH WVKEA+N YAEPIKMK+EEF++MMLVDACFIVEFFLL+ YG F E
Sbjct: 121 ISSKV---LVEKSHDWVKEAWNCYAEPIKMKDEEFIIMMLVDACFIVEFFLLY-YGSFHE 180

Query: 181 DG-------------------------------------------DEEEDSSF------- 240
           DG                                           D+ + SS        
Sbjct: 181 DGKLFNAELSLFYYGVFYEILLDLIKLENQVPFFLLQNLFDLMPKDKVDISSIIGGYKDS 240

Query: 241 -PSMINLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEPPPDYGQDKQEGENNNN 300
             S+I+LT    K FG+  +++   LY K PKHL+DFLSF+F P P             N
Sbjct: 241 PISLIDLTYMVLKEFGFVREYKINNLYHKNPKHLLDFLSFYFLPVPP------------N 300

Query: 301 NFLNFFGDLLQLCCFWNKKHCPEEMIRGSQ-SPPSITKLCEAGVTITPGDMNAGLCLIDI 360
           N              WN+K    ++ +  + SPP+ T+LCEAGVTI        LC ++I
Sbjct: 301 N--------------WNRKFDHVKISKQWRLSPPTTTELCEAGVTIKVAKRKNNLCFMNI 360

Query: 361 NFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLL 420
           +FKNGVL+IP I I+   EV+IRNV+AF+ +PAGN K+ A+QYV+FL+ LI TE+D+ LL
Sbjct: 361 SFKNGVLEIPPIVIEGTFEVLIRNVLAFEIFPAGNQKKYAIQYVTFLDDLISTEKDLCLL 420

Query: 421 VKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSK 463
           VK GVIIN   GSDK + E F +L+K        +F    +ALR HC+         W+K
Sbjct: 421 VKAGVIINDTGGSDKEVSELFNSLTKLVTTPLPSYFDDTSKALRVHCD-------GSWNK 474

BLAST of PI0012683 vs. ExPASy TrEMBL
Match: A0A5A7VF39 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002650 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 5.5e-89
Identity = 228/515 (44.27%), Postives = 290/515 (56.31%), Query Frame = 0

Query: 21  ISDAHEMLRGNVVIRIEEILKQLPEVNPKSSIYRISKELHELNDNAYTPQFISIGPF-HY 80
           I +A + L  NVVI I+++L Q+  +N   SIYRI K+L E+N  AYTPQ ISIGPF HY
Sbjct: 35  IIEAQQDLCDNVVISIQKMLNQMHSINGDCSIYRIPKQLREMNPKAYTPQLISIGPFHHY 94

Query: 81  DTRGDLLANEHYKVQGFISFLQRIDIN----KKENQGKITTSL--------LIFLVEKSH 140
             + D  A E YK+Q  ++FL+RI+ N    +++   K + SL        L  LVEK H
Sbjct: 95  RRQNDFKATEQYKLQALVNFLRRINNNNETMEEDQDVKKSRSLDDLLKIGTLKVLVEKVH 154

Query: 141 GWVKEAYNFYAEPIK-MKEEEFVVMMLVDACFIVEFFL---------------------- 200
            W+ E  N Y+EPI  M +  FV+MML+DACFIVE F+                      
Sbjct: 155 CWMTETRNCYSEPIDYMDDHNFVIMMLLDACFIVESFIQQYDASYPHDRKFPRIQDNVDF 214

Query: 201 LFCYGRFREDGDEE---------------------EDSSFPSMINLTIEA-FKWFGWGSD 260
           L  Y    +D   +                     + S   S INLT  A + WF     
Sbjct: 215 LLLYPETSQDISNDLIKLENQVPFFLLQHMFHMIPQHSPPVSFINLTYSALYDWFISTYK 274

Query: 261 FEEL-LYEKEPKHLVDFLSFFFEP---PPDYGQDKQEGEN------NNNNFLNFFGDLLQ 320
              + +   EPKHLV FLSF+F P     D  Q+KQE +N      NN NFL+FF  L  
Sbjct: 275 ICSVGIVPNEPKHLVHFLSFYFIPSHTTDDMIQNKQEIKNSENKKKNNYNFLSFFSTLF- 334

Query: 321 LCCFW----NKKHCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLK 380
            C  W    NKK   +E++    SPPSIT+LCE+GVTI     NA   L +I FKNGVLK
Sbjct: 335 CCQLWQNPDNKKKNDQELL----SPPSITELCESGVTIEKA-KNAKY-LTNITFKNGVLK 394

Query: 381 IPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIIN 440
           IP + I    E+M RN+IAF+ +PAGN+   A QY+ F++ LI TE+DV LLV  GVIIN
Sbjct: 395 IPHLHIYDEFELMFRNLIAFEQFPAGNENMYATQYILFMDDLISTEKDVRLLVNSGVIIN 454

Query: 441 LVDGSDKYILEWFKNLSKYTFNQPW-FHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKH 463
            + GSDK + E F NL K+    P  + F  I +ALR+HCN R       W+KAK +LKH
Sbjct: 455 NIGGSDKEVSELFNNLGKFVNQCPSPYMFNHISKALRKHCNGR-------WNKAKASLKH 514

BLAST of PI0012683 vs. NCBI nr
Match: XP_038884451.1 (UPF0481 protein At3g47200-like [Benincasa hispida] >XP_038884452.1 UPF0481 protein At3g47200-like [Benincasa hispida] >XP_038884453.1 UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 438.7 bits (1127), Expect = 6.1e-119
Identity = 271/522 (51.92%), Postives = 327/522 (62.64%), Query Frame = 0

Query: 1   MGNSEIE---QANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPK-SSIYRIS 60
           MG+S+IE   +AND  Y   E IIS+  + L  NVVI IEE L++LP VN    SIY++ 
Sbjct: 1   MGDSDIEIMKEANDESYDVVE-IISEVDQKLCDNVVICIEEKLERLPHVNANCKSIYQVP 60

Query: 61  KELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRID----INKKENQG 120
           K L E+ND AYTPQFISIGPFHY TR +L+ANEHYKVQGF +FL+R++    I   E+ G
Sbjct: 61  KGLREMNDKAYTPQFISIGPFHYGTRTNLIANEHYKVQGFFNFLRRLNNKYKIQSLEDSG 120

Query: 121 --KITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFC--- 180
             KITTSLL  LVEK+   VKEA+N YAEPI MKEEEF  MMLVDACFIVEF +L+    
Sbjct: 121 HVKITTSLLKVLVEKACCSVKEAHNCYAEPINMKEEEFFTMMLVDACFIVEFLILWVCEN 180

Query: 181 ----YGRFREDGDE---------------------------------------EEDSSFP 240
                G+F +  D                                        ++D    
Sbjct: 181 LLQRRGKFFQIQDNVDFSFYHGIEFDILIDLIKLENQVPFILLQNLFDLIPKLDDDPMIS 240

Query: 241 SMINLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEP-PPDYGQDKQEGENNNNN 300
           S+I LT  A K FG  S +E + LY KEPKHL+DFLSF+F P PPD  Q+KQ+   N   
Sbjct: 241 SLIRLTDIALKGFGLVSRYEIDDLYYKEPKHLLDFLSFYFLPLPPDDMQNKQDEIKNIEK 300

Query: 301 FLNFFGDLLQLCCFWNKK--HCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDI 360
             N F  LL   C WNKK  H      +   SPPS T+LCEAG+TI   D N  LCL +I
Sbjct: 301 KNNDFRKLLS--CLWNKKPDHEKNSYQQWRLSPPSTTELCEAGITIKVAD-NENLCLTNI 360

Query: 361 NFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLL 420
           +FKNGVL+IP I I+S  E+++RN+IAFDHYPAGN K+  +QYV FL+ LI TE+DVHLL
Sbjct: 361 SFKNGVLEIPPIIIESTFEIVVRNLIAFDHYPAGNQKQYVIQYVLFLDDLISTEEDVHLL 420

Query: 421 VKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSK 463
           VK GVIIN   GSDK I + F N SK+       HF  + +ALR HCN R       W+K
Sbjct: 421 VKAGVIINTFGGSDKEISDMFNNFSKFAIFPVTSHFDDMSQALRVHCNGR-------WNK 480

BLAST of PI0012683 vs. NCBI nr
Match: KAA0064937.1 (UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 424.1 bits (1089), Expect = 1.6e-114
Identity = 256/510 (50.20%), Postives = 311/510 (60.98%), Query Frame = 0

Query: 6   IEQANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSSIYRISKELHELNDN 65
           I+  +DI YK EE I  DA + L  NVV+ I+ IL+Q+P+VNPK  IYRISKE+ ELND 
Sbjct: 2   IQANHDITYKVEEPITGDADQELCVNVVMFIKNILEQVPQVNPKICIYRISKEVRELNDR 61

Query: 66  AYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKE------NQGKITTSLL 125
           AY PQFISIGPFHY TR DL+ANE+YK QGF +FL+RI IN ++      +Q KI T  +
Sbjct: 62  AYAPQFISIGPFHYHTRQDLIANEYYKYQGFNNFLRRISINNEQIESMEGSQVKINT--V 121

Query: 126 IFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLL-------------- 185
            FLVEK HG +KEA+N YA+ I M EEEFV MMLVDACFIVEF +L              
Sbjct: 122 KFLVEKCHGLMKEAWNCYADQINMMEEEFVGMMLVDACFIVEFLILLRGRVSNIQRIISK 181

Query: 186 ----FCYGRFRED-GD----EEEDSSF----------------------PSMINLTIEAF 245
               F  G   E  GD    E +   F                       S+++LT  AF
Sbjct: 182 IDSKFYQGALYEILGDLIKLENQVPFFLLQSLFDLITKDDLPLVGTHERASLMDLTCFAF 241

Query: 246 KWFGWGSDFEELLYEK-EPKHLVDFLSFFFEPPPDYGQDKQEGENNNNNFLNFFGDLLQL 305
           K F         +Y+K  PKHLVDF S FF  PP Y +D  +  +  + +          
Sbjct: 242 KIFVVAGYHFNYMYDKTTPKHLVDFSSIFFMSPPTYVEDNHKYVHTKDRW---------- 301

Query: 306 CCFWNKKHCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRIC 365
            C                  PS+T L EAGVTI P  + +  CL  I+F+NGVL IP I 
Sbjct: 302 -CI----------------SPSVTTLWEAGVTIQPSYVES--CLTSISFENGVLNIPHIN 361

Query: 366 IQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGS 425
           +    E+MIRN+IAFDHYPAGN    A++YVSFL  L++ EQDVHLLVKVGVI+N V G 
Sbjct: 362 MGKTFEIMIRNLIAFDHYPAGNKNMYAVEYVSFLHDLMKEEQDVHLLVKVGVIVNSVGGG 421

Query: 426 DKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTP 464
           DKYIL++F NLS+Y    P+  F +I E L  +CNRRRNR K YW+KAKVTL+HDYF TP
Sbjct: 422 DKYILDFFNNLSRYNMVSPFSQFGEIAETLHVYCNRRRNRAKRYWNKAKVTLRHDYFTTP 480

BLAST of PI0012683 vs. NCBI nr
Match: KAA0064941.1 (UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 407.9 bits (1047), Expect = 1.2e-109
Identity = 251/524 (47.90%), Postives = 327/524 (62.40%), Query Frame = 0

Query: 1   MGNSEIE--QANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSS-IYRISK 60
           M NSEIE  + N+I ++  E I  D  + +R  VVI I+++L  L  VN K + IY++ K
Sbjct: 1   MKNSEIEMIEDNNISHRVGEIISEDIDQKVRDIVVISIDKMLDGLSPVNSKCNIIYQVPK 60

Query: 61  ELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKK------ENQ 120
           EL E+ND AY PQFISIGPFHY TR DL+ANEHYK+QGF +FL+ I+IN+K      E+Q
Sbjct: 61  ELREINDKAYVPQFISIGPFHYRTRKDLIANEHYKLQGFYNFLRGININEKQVESLEEDQ 120

Query: 121 GKITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRF 180
            KI++ +   LVEKSH W+KEA+N YAEPIKM+EEEF++MMLVDACFIVEFFLL CYG F
Sbjct: 121 VKISSKV---LVEKSHSWMKEAWNCYAEPIKMEEEEFIIMMLVDACFIVEFFLL-CYGSF 180

Query: 181 REDG-----------------------------------------DEEEDSSFP------ 240
           +E                                           D ++ SS        
Sbjct: 181 QEGDRLFKFSLFYYGVFYEILLDLIKLENQVPFFLLQNLFDLIPKDNDDTSSIVDYEAGP 240

Query: 241 -SMINLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEPPP--DYGQDKQEGENNN 300
            S+I+LT  A K FG+  ++E   LY K P+H++D LSF+F P P  D   +KQ  +  N
Sbjct: 241 ISLIDLTYMALKAFGFVREYEINNLYHKNPEHILDLLSFYFLPVPHNDPDMNKQHDKRKN 300

Query: 301 N-NFLNFFGDLLQLCCFWNKKHCPEEMIRGSQ-SPPSITKLCEAGVTITPGDMNAGLCLI 360
             +FL+FF  L      WNKK   +++ +  + SPP+IT+L EAGVTI   +    LC +
Sbjct: 301 KADFLSFFRHL------WNKKSDHKKISKQRRLSPPTITELFEAGVTIKAAE-KENLCFM 360

Query: 361 DINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVH 420
           +I+FKNGVL+IP I I+   EV+IRNV+AF+ +PAGN K+ A+QYV+FL+ LI TE+D+ 
Sbjct: 361 NISFKNGVLEIPPIVIEGTFEVVIRNVLAFEIFPAGNQKKYAIQYVTFLDDLISTEKDLC 420

Query: 421 LLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYW 463
           LLVK GVI N   GSDK   E F +L+K        +F    +ALR HC+         W
Sbjct: 421 LLVKAGVITNDTGGSDKEASELFNSLTKLVTTPLSSYFDDTSKALRVHCD-------GSW 480

BLAST of PI0012683 vs. NCBI nr
Match: XP_008445182.1 (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo] >XP_008445184.1 PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo] >XP_016899950.1 PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-107
Identity = 255/540 (47.22%), Postives = 308/540 (57.04%), Query Frame = 0

Query: 1   MGNSEIEQA---NDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSS-IYRIS 60
           M NSEI +    NDI        IS+  E +  NVVI I++IL  LP +NPK   IY++S
Sbjct: 1   MENSEIIETKVENDICDDELGETISEI-EKVCDNVVISIDKILGGLPRINPKCHIIYQVS 60

Query: 61  KELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQGKITT 120
           KEL E+ND AY PQFISIGPFH+ TR DL+ANEHYK+QGF +FL RI+     N  +I +
Sbjct: 61  KELREMNDKAYAPQFISIGPFHHRTRNDLIANEHYKLQGFNNFLHRIN-----NYEQIES 120

Query: 121 SLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGD 180
           S     V+K HGWVKEA+N YAEPI M EEEFV+MMLVDACFI+EFF+L     +  D  
Sbjct: 121 SK--EFVKKCHGWVKEAWNCYAEPINMNEEEFVLMMLVDACFILEFFILLIDDHYGGDYL 180

Query: 181 EEEDS-------------------------------------------------SFPSMI 240
            E D                                                   FPS+I
Sbjct: 181 FEADQIFQIQDMVDFSFYRGVFFEILIDLIKLENQVPFFLLQNLFDLMPKHDVPMFPSLI 240

Query: 241 NLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEP--PPD-----YGQDKQEGENN 300
           ++T E   WFG+   ++   LY K+PKHL+DFLSF+F P  P D        +++  + N
Sbjct: 241 DITSEILTWFGFVGKYKINDLYHKKPKHLLDFLSFYFFPLLPNDDHIRFKQNERKNSDQN 300

Query: 301 NNNFLNFFGDLL---------------QLCCFWNKKHCPEEMIRGSQ---SPPSITKLCE 360
           NNN L FF  L                 LCCF NK+    E    +    SPPSIT+LCE
Sbjct: 301 NNNLLRFFRPLFPAHWLKKNNDSFGVPSLCCFSNKEAETREKDSENYFRLSPPSITELCE 360

Query: 361 AGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSAL 420
           AGVTI        LC ++I FKNGVL+IP I I    EV+IRNVIAFD YPAGN+K  A+
Sbjct: 361 AGVTIKAA-KREDLCFMNIGFKNGVLEIPCIDIDCTFEVVIRNVIAFDQYPAGNEKMYAI 420

Query: 421 QYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGE 462
            YV FL+ LI TEQD HLL K G+IIN   GSDK I E F   SK+       HF  I +
Sbjct: 421 HYVLFLDDLINTEQDAHLLTKAGIIINTFGGSDKDITEMFNRFSKFVTFPICSHFDDINK 480

BLAST of PI0012683 vs. NCBI nr
Match: XP_031737062.1 (UPF0481 protein At3g47200 [Cucumis sativus])

HSP 1 Score: 395.6 bits (1015), Expect = 5.9e-106
Identity = 244/522 (46.74%), Postives = 312/522 (59.77%), Query Frame = 0

Query: 1   MGNSEIEQANDIPYKPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPK-SSIYRISKEL 60
           M NSEIE  ++           +  + +R NVVI I+++L+ LP VNPK ++IY++ KEL
Sbjct: 1   MENSEIEIMSE-----------NIDQNVRDNVVISIDKMLEGLPRVNPKCNTIYQVPKEL 60

Query: 61  HELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINK------KENQGK 120
            E+ND AY PQFISIGPFHY TR DL+ANEHYK+QGF +FL RI I        +ENQ  
Sbjct: 61  REINDKAYVPQFISIGPFHYRTRKDLIANEHYKLQGFFNFLGRISIINSHIQLLEENQVN 120

Query: 121 ITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFRE 180
           I++ +   LVEKSH WVKEA+N YAEPIKMK+EEF++MMLVDACFIVEFFLL+ YG F E
Sbjct: 121 ISSKV---LVEKSHDWVKEAWNCYAEPIKMKDEEFIIMMLVDACFIVEFFLLY-YGSFHE 180

Query: 181 DG-------------------------------------------DEEEDSSF------- 240
           DG                                           D+ + SS        
Sbjct: 181 DGKLFNAELSLFYYGVFYEILLDLIKLENQVPFFLLQNLFDLMPKDKVDISSIIGGYKDS 240

Query: 241 -PSMINLTIEAFKWFGWGSDFE-ELLYEKEPKHLVDFLSFFFEPPPDYGQDKQEGENNNN 300
             S+I+LT    K FG+  +++   LY K PKHL+DFLSF+F P P             N
Sbjct: 241 PISLIDLTYMVLKEFGFVREYKINNLYHKNPKHLLDFLSFYFLPVPP------------N 300

Query: 301 NFLNFFGDLLQLCCFWNKKHCPEEMIRGSQ-SPPSITKLCEAGVTITPGDMNAGLCLIDI 360
           N              WN+K    ++ +  + SPP+ T+LCEAGVTI        LC ++I
Sbjct: 301 N--------------WNRKFDHVKISKQWRLSPPTTTELCEAGVTIKVAKRKNNLCFMNI 360

Query: 361 NFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLL 420
           +FKNGVL+IP I I+   EV+IRNV+AF+ +PAGN K+ A+QYV+FL+ LI TE+D+ LL
Sbjct: 361 SFKNGVLEIPPIVIEGTFEVLIRNVLAFEIFPAGNQKKYAIQYVTFLDDLISTEKDLCLL 420

Query: 421 VKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSK 463
           VK GVIIN   GSDK + E F +L+K        +F    +ALR HC+         W+K
Sbjct: 421 VKAGVIINDTGGSDKEVSELFNSLTKLVTTPLPSYFDDTSKALRVHCD-------GSWNK 474

BLAST of PI0012683 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 165.6 bits (418), Expect = 9.3e-41
Identity = 132/454 (29.07%), Postives = 214/454 (47.14%), Query Frame = 0

Query: 24  AHEMLRGN----VVIRIEEILKQLPEVNPKSSIYRISKELHELNDNAYTPQFISIGPFHY 83
           A+E +  N    +V  I+  L  L  ++ K  IY++  +L  LN +AYTP+ +S GP H 
Sbjct: 263 AYERMNQNEGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHR 322

Query: 84  DTRGDLLANEHYKVQGFISFLQRIDINKKENQGKITTSLLIFLVEKSHGWVKEAYNFYAE 143
             + +L A E  K +  +SF+ R            T S L  LV  +  W + A + YAE
Sbjct: 323 G-KEELQAMEDQKYRYLLSFIPR------------TNSSLEDLVRLARTWEQNARSCYAE 382

Query: 144 PIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGDEEEDSSFPSMINLTIEAFKWFGW 203
            +K+  +EFV M++VD  F+VE  L   Y R R     E D  F + + +T         
Sbjct: 383 DVKLHSDEFVEMLVVDGSFLVELLLRSHYPRLR----GENDRIFGNSMMITDVCRDMI-- 442

Query: 204 GSDFEELLYEKEPKHLVD-----FLSFFFEPPPDYGQDKQE------GENNNNNFL---N 263
                 L+  + P  +V       L+++ +  P   Q  Q          ++  F+    
Sbjct: 443 ------LIENQLPFFVVKEIFLLLLNYYQQGTPSIIQLAQRHFSYFLSRIDDEKFITEPE 502

Query: 264 FFGDLLQLCCFWNKKHCPEEMIRGSQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNG 323
            F DLL+ C         E       + P  T+L  AGV   P + ++  CL+DI+F +G
Sbjct: 503 HFVDLLRSCYLPQFPIKLEYTTVKVDNAPEATELHTAGVRFKPAETSS--CLLDISFADG 562

Query: 324 VLKIPRICIQSRVEVMIRNVIAFDHYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGV 383
           VLKIP I +    E + +N+I F+     N  ++ L Y+  L   I++  D  LL+  G+
Sbjct: 563 VLKIPTIVVDDLTESLYKNIIGFEQCRCSN--KNFLDYIMLLGCFIKSPTDADLLIHSGI 622

Query: 384 IINLVDGSDKYILEWFKNLSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTL 443
           I+N + G+   +   F ++SK       F+F  + E L+ +CN       + W++ K  L
Sbjct: 623 IVNYL-GNSVDVSNLFNSISKEVIYDRRFYFSMLSENLQAYCN-------TPWNRWKAIL 679

Query: 444 KHDYFNTPWAAISVIAASFLIILTLLQTIFSAIS 460
           + DYF+ PWA  SV AA  L++LT +Q++ S ++
Sbjct: 683 RRDYFHNPWAVASVFAALLLLLLTFIQSVCSILA 679

BLAST of PI0012683 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-27
Identity = 139/484 (28.72%), Postives = 208/484 (42.98%), Query Frame = 0

Query: 5   EIEQANDIPYKPEET------IISDAHEM-LRGNVVIRIEEILKQLPEVNPKS----SIY 64
           E+E    I  KP ET       I D +E  LR   VI + + +K L +    S     IY
Sbjct: 48  ELENIEVIEEKPRETQVESVVSIEDKNEQKLREIWVISLNDKMKTLGDNATTSWDNLCIY 107

Query: 65  RISKELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQGK 124
           R+   L E +  +Y PQ +SIGP+H+  +  L+  E +K +          +N    + K
Sbjct: 108 RVPPYLQENDTKSYMPQIVSIGPYHHGHK-HLMPMERHKWRA---------VNMVMARAK 167

Query: 125 ITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFRE 184
               + I  +++     ++A   Y  PI M   EF+ M+++D  FI+E F     G F+E
Sbjct: 168 HDIEMYIDAMKELE---EKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEG-FQE 227

Query: 185 DGDEEEDSSF------PSMINLTIEAFKWFGWGSDFEELLYEKEPKHL----VDFLSFFF 244
            G    D  F       S+    +       W S  + LL  + P  L    V     FF
Sbjct: 228 IGYAPNDPVFGMRGLMQSIRRDMVMLENQLPW-SVLKGLLQLQRPDVLDKVNVQLFQPFF 287

Query: 245 EP-PPDYGQDKQEG-----ENNNNNFLNFFGDLLQLCCFWNKKHCPEEMIRGSQSPPSIT 304
           +P  P      +EG     +      L   G   +     NK+  P+++I        +T
Sbjct: 288 QPLLPTREVLTEEGGLHCLDVLRRGLLQSSGTSDEDMSMVNKQ--PQQLIH------CVT 347

Query: 305 KLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFDHYPAGNDK 364
           +L  AGV     +        DI FKNG LKIP++ I    + +  N+IAF+     + K
Sbjct: 348 ELRNAGVEFMRKETGH---FWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIKSSK 407

Query: 365 RSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNLSKYTFNQPWFHFL 424
           +    Y+ F++ LI + +DV  L   G+I N + GSD  + + F  L K     P   +L
Sbjct: 408 K-ITSYIIFMDNLINSSEDVSYLHHYGIIENWL-GSDSEVSDLFNGLGKEVIFDPNDGYL 467

Query: 425 KIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAAISVIAASFLIILTLLQTIFSA 462
               AL    N    R    W+  K TL+H YFN PWA  S IAA  L+I T  Q+ F+ 
Sbjct: 468 ---SALTGEVNIYYRRK---WNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAV 497

BLAST of PI0012683 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 117.9 bits (294), Expect = 2.2e-26
Identity = 133/493 (26.98%), Postives = 204/493 (41.38%), Query Frame = 0

Query: 15  KPEETIISDAHEMLRGNVVIRIEEILKQLPEVNPKSS-----IYRISKELHELNDNAYTP 74
           KPEET         R   VI I + ++Q    +  +S     IYR+ + L E N  +Y P
Sbjct: 108 KPEET---------REEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFP 167

Query: 75  QFISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQGKITTSLLIFLVEKSHGW 134
           Q +S+GPFH+  +  LL  + +K +     + R            T   +   ++     
Sbjct: 168 QTVSLGPFHHGNK-HLLPMDRHKWRAVNMVMAR------------TKHDIEMYIDAMKEL 227

Query: 135 VKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGDEEEDSSFP----- 194
              A   Y  PI +   +F  M+++D CF++E F     G F E G +  D  F      
Sbjct: 228 EDRARACYEGPIDLSSNKFSEMLVLDGCFVLELFRGADEG-FSELGYDRNDPVFAMRGSM 287

Query: 195 -----SMINLTIEAFKWFGWGSDFEELLYEKEPKHLVDFLSF-FFEP--PPDYGQDKQEG 254
                 M+ L       F      E  L ++    LV  L+  FF+P  P D    K + 
Sbjct: 288 HSIQRDMVMLE-NQLPLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDD 347

Query: 255 ENNNNNFLN---------------FFGDLLQLCC-----------FWNKKHCPEEMIRGS 314
               + F N               F  +LL+ C             W  +   +   R  
Sbjct: 348 SLEQDKFFNPIADKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADK---RQQ 407

Query: 315 QSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFD- 374
           Q    +T+L EAG+       +      DI FKNG L+IP++ I    + +  N+IAF+ 
Sbjct: 408 QLIHCVTELREAGIKFRTRKTDR---FWDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQ 467

Query: 375 -HYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNLSKYT 434
            H  + ND  S   Y+ F++ LI++ +DV  L   G+I + + G+D  + + F  L +  
Sbjct: 468 CHIDSSNDITS---YIIFMDNLIDSSEDVRYLHYCGIIEHWL-GNDYEVADLFNRLCQEV 527

Query: 435 FNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAAISVIAASFLIIL 462
              P   +L     L    +R  +R    W+  K  LKH YFN PWA  S  AA  L++L
Sbjct: 528 AFDPQNSYL---SQLSNKVDRNYSRK---WNVLKAILKHKYFNNPWAYFSFFAALVLLVL 560

BLAST of PI0012683 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 117.1 bits (292), Expect = 3.8e-26
Identity = 128/495 (25.86%), Postives = 214/495 (43.23%), Query Frame = 0

Query: 16  PEETIISDAHEMLRGNVVIRIEEILKQLPEVNP-----KSSIYRISKELHELNDNAYTPQ 75
           P   +I ++ +  R + VI I + L+Q    +      K  IYR+   L E ++ +Y PQ
Sbjct: 64  PAIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQ 123

Query: 76  FISIGPFHYDTRGDLLANEHYKVQGFISFLQRIDINKKENQG-KITTSLLIFLVEKSHGW 135
            +S+GP+H+  +  L + + +K +     L      K+ NQG K+    +  L EK+   
Sbjct: 124 TVSLGPYHHGKK-RLRSMDRHKWRAVNRVL------KRTNQGIKMYIDAMRELEEKARA- 183

Query: 136 VKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFFLLFCYGRFREDGDEEEDSSFP----- 195
                  Y  P+ +   EF+ M+++D CF++E F     G F E G    D  F      
Sbjct: 184 ------CYEGPLSLSSNEFIEMLVLDGCFVLELFRGAVEG-FTELGYARNDPVFAMRGSM 243

Query: 196 -----SMINLTIEAFKWFGWGSDFEELLYEKEPKHLVDFLSF-FFEP--PPD-----YGQ 255
                 M+ L       F      E  L  +    LV  L+  FF+P  P D      GQ
Sbjct: 244 HSIQRDMVMLE-NQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQ 303

Query: 256 DKQEGENNNNNFLNFFGDLLQLCCF----------------------WNKKHCPEEMIRG 315
            K E     +   + F D+ +L C                       W++     +  R 
Sbjct: 304 SKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVADK-RR 363

Query: 316 SQSPPSITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEVMIRNVIAFD 375
            Q    +T+L EAG+       +      D+ FKNG L+IPR+ I    + +  N+IAF+
Sbjct: 364 QQLIHCVTELKEAGIKFRRRKTDR---FWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFE 423

Query: 376 --HYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYILEWFKNL-SK 435
             H  + ND  S   Y+ F++ LI++ +DV  L   G+I + + GSD  + + F  L  +
Sbjct: 424 QCHIDSSNDITS---YIIFMDNLIDSHEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQE 483

Query: 436 YTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAAISVIAASFLI 462
             F+    +  ++   +  + + +       W+  + TLKH YFN PWA +S  AA  L+
Sbjct: 484 VVFDTEDSYLSRLSIEVNRYYDHK-------WNAWRATLKHKYFNNPWAIVSFCAAVILL 527

BLAST of PI0012683 vs. TAIR 10
Match: AT2G28580.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-25
Identity = 122/442 (27.60%), Postives = 201/442 (45.48%), Query Frame = 0

Query: 52  IYRISKELHELNDNAYTPQFISIGPFHYDTRGDLLANEHYKVQ-GFISFLQRIDINKK-- 111
           IYR+   L ++N  AYTPQ + IGP H+  + + L  + YK    ++++L  ++++KK  
Sbjct: 111 IYRVPDRLRKVNPEAYTPQMLLIGPLHHSKKAEAL--KRYKTDLRYLNYL-NMELHKKKC 170

Query: 112 -----ENQGKITTSLLIFLVEKSHGWVKEAYNFYAEPIKMKEEEFVVMMLVDACFIVEFF 171
                +  G         ++E +  +++++Y      I +  ++FV M+L D+ FI+ FF
Sbjct: 171 LDSIADIYGDQPVKEFRRIIEINEKFIRDSY--AESTIWINTKDFVEMILHDSVFILLFF 230

Query: 172 LLFCYGRFREDGDEEEDSSF--PSMINLTIEAFKWFGWGSDFEEL-LYEKEPKHLVDFLS 231
           +         + +++ED  F    +IN T          +  E+L L E +  + +  L 
Sbjct: 231 I---QTGSTLNFNKKEDILFNQSRLINAT----------AILEDLILLENQLPYAL--LE 290

Query: 232 FFFEPPPDYGQDKQ------------EGENNNNNFLNFFGDLLQLCCFWNKKHCPEEMIR 291
             FEP       K+            EG+         F DL + C   +     EE I 
Sbjct: 291 KLFEPFSSNVNTKETFRDITLRAFRFEGKIKEEVRFQHFTDLFR-CVRVSTLSLTEEQIN 350

Query: 292 GSQSPP--------SITKLCEAGVTITPGDMNAGLCLIDINFKNGVLKIPRICIQSRVEV 351
            +++ P        +  KL  AGV     D    L L+ I FK+G+LK+P   ++   E 
Sbjct: 351 IAKNEPPKSRKIMYNADKLDSAGVNFVNVDEENDLSLV-ITFKDGILKMPCFTVEDNTER 410

Query: 352 MIRNVIAFD--HYPAGNDKRSALQYVSFLEKLIETEQDVHLLVKVGVIINLVDGSDKYIL 411
           ++RN++A +  HYP          Y+SFL+ LI T+QDV LL K G++ N + G    + 
Sbjct: 411 VVRNLMALEQCHYPR---TTFVCDYISFLDFLINTDQDVDLLAKKGIVKNWL-GHQGSVT 470

Query: 412 EWFKN--LSKYTFNQPWFHFLKIGEALREHCNRRRNRTKSYWSKAKVTLKHDYFNTPWAA 459
           E      L    F     H+  I E L +H + R NR+         TL+  YF   W  
Sbjct: 471 EMVNKLCLGLVDFGS---HYSDIVENLNKHYDNRLNRSVG-------TLRRVYFKDLWTG 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD533.3e-1421.44UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7VD327.6e-11550.20UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00255... [more]
A0A5A7VGS65.6e-11047.90UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00259... [more]
A0A1S4DW655.2e-10847.22UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488289 PE=4 SV=1[more]
A0A0A0LQ572.9e-10646.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380610 PE=4 SV=1[more]
A0A5A7VF395.5e-8944.27UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00265... [more]
Match NameE-valueIdentityDescription
XP_038884451.16.1e-11951.92UPF0481 protein At3g47200-like [Benincasa hispida] >XP_038884452.1 UPF0481 prote... [more]
KAA0064937.11.6e-11450.20UPF0481 protein [Cucumis melo var. makuwa][more]
KAA0064941.11.2e-10947.90UPF0481 protein [Cucumis melo var. makuwa][more]
XP_008445182.11.1e-10747.22PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo] >XP_008445184.1 PREDICT... [more]
XP_031737062.15.9e-10646.74UPF0481 protein At3g47200 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G31980.19.3e-4129.07unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50160.11.2e-2728.72Plant protein of unknown function (DUF247) [more]
AT3G50130.12.2e-2626.98Plant protein of unknown function (DUF247) [more]
AT3G50120.13.8e-2625.86Plant protein of unknown function (DUF247) [more]
AT2G28580.14.2e-2527.60Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 52..175
e-value: 5.1E-23
score: 82.0
coord: 207..448
e-value: 2.7E-42
score: 145.5
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 32..173
coord: 181..459
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 32..173
coord: 181..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012683.1PI0012683.1mRNA