PI0012409 (gene) Melon (PI 482460) v1

Overview


Name	PI0012409
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	tRNA 4-demethylwyosine synthase (AdoMet-dependent)
Location	chr03: 1811450 .. 1817998 (-)
RNA-Seq Expression	PI0012409
Synteny	PI0012409

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCAAAATGATTTAGGGCAACCATCCAATTTACATTTTGGGCTTGGGCTTTGTTCACTTCGGTTCGCAATTGGGCAGCTCGCTCCGACCTGGATGCTCCGGCGAGGCCACCGTCTTCTTCTTCTTTTTCTTCTTCTTCAATTTCCAATATTCTCTTGTGAGTCGCAAAAAAATGAAGCTAAGGATCTTCGCTTTCGGTTTACGTTCGATCAATTAACCTAATAGCATCTCAAAACCAATCAATTCGCCATGTCCTTTTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGGAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAATCCCAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTTTGCTCAACCTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTATGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTAGCTAATTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCTAAGCGGATGAAAGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTGTGAAGGTTGATACCGAGGGAAATGGGGTGGAGTTTTTTTCTGGGACTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAATCTACGAATGTAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGTAAGTTCGTTGGCTGTTTATATGCATATTTGTAATCATAAATATGGTTCTACGCATATGCGTTTTCCAAATTGTTCGAATAGCTGCTTTTTTAAGGCCATTCTGAGTGTCTTGTGAATCAATACAAATTTGAACTGACTTGATAATTTGTTCTTGCAAAGTGCTAGTTGGGTTGAATTAGAAGCCTTACCTCTTTTTCTTTTGTTGTTTGGTTGGCTCTTTGAATGATTTTTCTTATGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGGGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGGTAATGTCCCACTTTATATGCTATTTTGTTTGGCCATAGCGTGTCTTATTTCCTTTTCATGGCCATTATAAATTTGTAATTCTTGATGCTGTAATGCATTAAGTGGCAATGGCATGGAGATCCCCCTTCATTAAAATGTATGCCACTTTACTTTGGTAAATATATAATTTCAACTCTTGAGGAGGATGGATGATAGAATATGATTGAATACTGACTAGGTGAATATAAGAATAGATTGGCAGTGGCCTTCAAAGCACTGCAGAATTCACAAATCAAATGGAGATCACTTTGTTTGATATTCTTTGGTCGGCCAAATATTATATCCAGCTACTTCTAATTTCATTCTGGCAATCTTTCTCGGTTTGCCTGCGTGACTGATATCTTAACCTCTTTTAGTTGTTACGCTGGTGCAAGATGTGTGTTCATAGGTCCTTTTGTTTGACTTGCATTATGAGTTCTTATTCATTCGTGTTAAGTTCACTTCTCTGGCCTCTGGGAAATTTCAAGTTGACTCTTCACTTTCCTTTCTCTCTCAACAAAGCTTTTCATAAGCTCTTGACTTTTTTAATAGACTGGTAAAAACTATCTATTGATCAGTGCAGTTGCTCACAATAACTTTTTTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATAGATTTACACAAAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTTAGTGCTCCTGCCTTACCGCACTGGTTTGAGTAATATTTTTATGAAGAATGTCTTTTCTTGGATATACTCAATTGTTTATGTGATTTTCTGATTGTGGGATCCGAAATGTTGGTAAATTATCAATATTTTTAACTTTCATAAAACCGTGTTGGCGTGGCATGAGTTTTGTGGCTAAAAAGAATCGGGAGCAGTAGAGACCGAAAGTAACCAGAGTATTATTCAGTTGAATACAGGTTACTGAGGGTCTTGTGAGGGTATAGAAGTGCGTTAGCATAGCATGATTTTTGTGGCTAAACAGAGATCAAGAGTAGTAGGGACCAAAAGTAACCTGTATTCTATGCAGTTGAATTTTCCTTTGGGAGTTTTAGAGATCTTTCAAAGGAATTTAAGCAATATGTTTAATCTAAGATTCTTTTATCTGGCTGCTTGAGAATGCAAAGTTGAGGTATTTTATTTTACAGTAGCCAATAGCCATCAGTGAGAGTATTGTGAGGATGCTTGATATGCAAGTTATTTTTCATATACTTTTTACTTGTATATTTCAAACTTTTTTTTTGGGCAGTAATCTATGAAATGTAATCGCTGAGTTTATGTTCATATAAAACTTTTTATTTTATTTATTTATTTATTTTATTCAGAAAGAACAAAGAAGTACATGTCATGAAATTTGAAAGGCAATGACCAAACCTTGCTTGCTTAAGTGCAAAGCTTGCTCCCTGACAAAACAAGTGTAAACCTAATTTTAGTTATTCAGATCCACTCATGTTAATAAAATAGAAGTTAATGAACCATGAATGCTTTAAGGGGTAAAAGCATATGGTAGTTGGGATTTTAGTGGCAAGGTGAGTCATTAATGCATGCCATCTTGTTGATAAGCAATGTCTTCCATCTTCCTGCAGGTGTTACGCAGGAGAAGTTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAATTTGCTGAAGCCAGTCACCCAGGTTTGCGTGCTTTCAAATTTTTGTTTTCTAAGTAACATTAAATGTCAGGATTATATTCTTCAATCATGTTACTAGATTACATATCTAATTTTTATGATGCATGCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGTAAGTTTCCTCATGCCCTCACCTCTTTCTTAGCTTGTCTCTTAAGCCAGGCTTCATTTGAACTAATGAGAGAAGTAGATCTTGGGGGGTGTTTTATATCTTGTTGCTTTGTTGCCGTAATCTGCTTTGTAGTTTGCTGTTCTTTTTCATGTAGAGATGCAAAAGATTTGCAGTTTCTTTACAAAAATCCTAAAAGACATTCTAGTTTCTCGAGTACATTGTCAAATAGGCTGGATAAGGATCAGATATTTCTTGTCAATTTGTCATCACACACCATTGCTTAAGTTCTTTGACTATCACAATTAATCTTGGCCTTACATTCTGTGGATCGAAGGAAATATTCTTTAAATAACTGATTTGTGTGCTCCCATAATGTTGCAGGATTCTTTAAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGAGGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCGTGAGTTTGTATTTATTTATTTTGCATTCGATCGCAGGATATTGATTTGAAAATTGTGGGAGCTTAACAAATCTAATGTTCTTCTGTAGATCTAATACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGTGTTCTTTTAGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGAGAAATTCCATGACCTGGTTTGTATAGACGACAACTAATTTACAATGAAACTTTAAAATGTTGAAGCTCCTTCCATAAATTTTATCCTTTTTGAAAATTTTAGGTGGCCTCTGGAAAACCTTTTGATAGCAAAGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAAACCAAAACCCACATCTTGACAGCTTTGAATGTCCGGGTCAGTTTTGTTTAATGGGTTAATCAATTTTCATTGAGGATTTCTGTGGTTTATATTGTTCTATTGTGAAACAGCCTAGCTTGCCATTATTTATTTTTAAAAAGTGTCTTGCGTCGCATTTCCATACATATACATGTTTAAACTTGTTCTACTTTCCTAGTTATAGAAAATATAGAGCTTGCTTGGTAATCAAATAATATTTTAGTCATTCTATTTTTAAATGTGTAACAATTTATTATTGTAGCTTGAAATTTGTCACAATTTTATCCCCATCATAAAAAATTCCATCAAAATTAAGTGTTAATTTTTATTACATAATGACTTAATATTTGTTTTTTCTATAAAGTCCTGTATCAATTTACATATGTAAGAAATCTTATTGAATCTTAACAAGTTTTTCATGGACCATATTGTAGTAAATTTTAAAATACAAGGACTAAGTTGTTATGTAGTAAAATTCATAGGCTAATTTGTTACACCAGCAAAACTGTAGGGACTAAATCATTAGAGAATAAAGTTCATGTACTATATTGTTATTTTCATGGACTAAAAGTTTTGTTTAACCAATTTATCTTTACACTTAATTTCCTTGATTGTTTCTGAGAAATGTTCTTGGTTAAGAAAGAAAGCAAAGTTAGCCGATAACTAAGACAACGTCTTCTCTTACCTTTTCTCTTGTTTTCTCTAAGACAATTGTATTTTTGGTGTATTTATGCTTAGTTATGATTTTTTTTCATAAATGGAAACGTTTGTAGGCCCCATCCTTATCATTCTCCTCAGACCTTAAGAGACCCATAGAGGTGCTTGAATTTTCCTAACACTAACTGCTAACGTTCATCGTTCTTCCAGAGTCATGTCACCATCCATTTAAGGTTGATTTACCTTCTTAGAGTTAGCAGCCGACGAATAACATTATTTCAACCTCAGAAAGCGGGAAGGGCACCATGGCTGTACGTGTCCCATCAGTAGATATAATCCATGAGGATAGGAACGGATCCTGGCATGTTGCCATCTACCTACCTTCATACTAAAAAAATAGTTGTACCTACAATATTTACTGGATCCAACCTTCAAAATCAAAGAATAAGTCCAATCAAATCAAGATCTGTTTTACCTTCAGTAAGAATAAGTCCAGATAAATCTAGATCTGTGTTAGTAGTACTGTAGTAGTATAGTTCTTAAAGGTTTTCATGATATACAACTGGTCTGGTGTATATTATCCCTGTTATAAATGTTGAAACAGATACCTCTTATAGGACTGTACCGAAGCAAAGCTTGTCATTGCCAACTCCATTGGATCAGTTCAAGTGCAACCCGTGGAGAATAAAGTACTTTAATTGGTTGCACGTGGCGAGGGAGTAAAGGTATAGAGTCTTGAAAATGGTCACTCAGCATTACTTTGAGAACTCCAAATTTACTTCCAGTATCTCTAAGTTTGAATTCTTGATGGATGTTGAAATCTTAGGATGTATATTGTTGCATTAAAGAATGGTTTGTGGTACAGAGTTTTCTGGGGCTGCTGGTGAATAACCCACGGTAAAAGATGGAGAGACAAGATCCTCATCTGCTGGTCAATTGTCCCCAGGTAAATTTATTTGGTGATAAGTGAAATTGACCTTAGAACATAGTTTCTTCTATTATTTGGTCGTTTTTTTTTGGGAGGTCTACATACCATGAACCGGAGAACAAAGGATGACCATTGATTTAGATATATTTAAAGGCTTAGAAATTCAGAATTAGTGTGTTGTTTCGTAGGATTTATCCCCTAAATTTTGGATTTCAATTCTAAATTTATGAACTCTTTTTAGATACGAATTTTAAGCTTTTTTGTTTGTTTATCTTTTCACTAAAAGAACGATTGATGGAATATATGAAAATCCCAGAAATTAAAAATCTGGAACCCAGTGGTTATTTAA

mRNA sequence

TTTCAAAATGATTTAGGGCAACCATCCAATTTACATTTTGGGCTTGGGCTTTGTTCACTTCGGTTCGCAATTGGGCAGCTCGCTCCGACCTGGATGCTCCGGCGAGGCCACCGTCTTCTTCTTCTTTTTCTTCTTCTTCAATTTCCAATATTCTCTTGTGAGTCGCAAAAAAATGAAGCTAAGGATCTTCGCTTTCGGTTTACGTTCGATCAATTAACCTAATAGCATCTCAAAACCAATCAATTCGCCATGTCCTTTTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGGAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAATCCCAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTTTGCTCAACCTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTATGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTAGCTAATTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCTAAGCGGATGAAAGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTGTGAAGGTTGATACCGAGGGAAATGGGGTGGAGTTTTTTTCTGGGACTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAATCTACGAATGTAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGGGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATAGATTTACACAAAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTGTTACGCAGGAGAAGTTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAATTTGCTGAAGCCAGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGATTCTTTAAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGAGGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCATCTAATACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGTGTTCTTTTAGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAAGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAAACCAAAACCCACATCTTGACAGCTTTGAATGTCCGGGTCAGTTTTGTTTAATGGGTTAATCAATTTTCATTGAGGATTTCTGTGGTTTATATTGTTCTATTGTGAAACAGCCTAGCTTGCCATTATTTATTTTTAAAAAGTGTCTTGCGTCGCATTTCCATACATATACATGTTTAAACTTGTTCTACTTTCCTAGTTATAGAAAATATAGAGCTTGCTTGGTAATCAAATAATATTTTAGTCATTCTATTTTTAAATGTGTAACAATTTATTATTGTAGCTTGAAATTTGTCACAATTTTATCCCCATCATAAAAAATTCCATCAAAATTAAGTGTTAATTTTTATTACATAATGACTTAATATTTGTTTTTTCTATAAAGTCCTGTATCAATTTACATATGTAAGAAATCTTATTGAATCTTAACAAGTTTTTCATGGACCATATTGTAGTAAATTTTAAAATACAAGGACTAAGTTGTTATGTAGTAAAATTCATAGGCTAATTTGTTACACCAGCAAAACTGTAGGGACTAAATCATTAGAGAATAAAGTTCATGTACTATATTGTTATTTTCATGGACTAAAAGTTTTGTTTAACCAATTTATCTTTACACTTAATTTCCTTGATTGTTTCTGAGAAATGTTCTTGGTTAAGAAAGAAAGCAAAGTTAGCCGATAACTAAGACAACGTCTTCTCTTACCTTTTCTCTTGTTTTCTCTAAGACAATTGTATTTTTGGTGTATTTATGCTTAGTTATGATTTTTTTTCATAAATGGAAACGTTTGTAGGCCCCATCCTTATCATTCTCCTCAGACCTTAAGAGACCCATAGAGGTGCTTGAATTTTCCTAACACTAACTGCTAACGTTCATCGTTCTTCCAGAGTCATGTCACCATCCATTTAAGGTTGATTTACCTTCTTAGAGTTAGCAGCCGACGAATAACATTATTTCAACCTCAGAAAGCGGGAAGGGCACCATGGCTGTACGTGTCCCATCAGTAGATATAATCCATGAGGATAGGAACGGATCCTGGCATGTTGCCATCTACCTACCTTCATACTAAAAAAATAGTTGTACCTACAATATTTACTGGATCCAACCTTCAAAATCAAAGAATAAGTCCAATCAAATCAAGATCTGTTTTACCTTCAGTAAGAATAAGTCCAGATAAATCTAGATCTGTGTTAGTAGTACTGTAGTAGTATAGTTCTTAAAGGTTTTCATGATATACAACTGGTCTGGTGTATATTATCCCTGTTATAAATGTTGAAACAGATACCTCTTATAGGACTGTACCGAAGCAAAGCTTGTCATTGCCAACTCCATTGGATCAGTTCAAGTGCAACCCGTGGAGAATAAAGTACTTTAATTGGTTGCACGTGGCGAGGGAGTAAAGAGTTTTCTGGGGCTGCTGGTGAATAACCCACGGTAAAAGATGGAGAGACAAGATCCTCATCTGCTGGTCAATTGTCCCCAGGTAAATTTATTTGGTGATAAGTGAAATTGACCTTAGAACATAGTTTCTTCTATTATTTGGTCGTTTTTTTTTGGGAGGTCTACATACCATGAACCGGAGAACAAAGGATGACCATTGATTTAGATATATTTAAAGGCTTAGAAATTCAGAATTAGTGTGTTGTTTCGTAGGATTTATCCCCTAAATTTTGGATTTCAATTCTAAATTTATGAACTCTTTTTAGATACGAATTTTAAGCTTTTTTGTTTGTTTATCTTTTCACTAAAAGAACGATTGATGGAATATATGAAAATCCCAGAAATTAAAAATCTGGAACCCAGTGGTTATTTAA

Coding sequence (CDS)

ATGTCCTTTTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGGAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAATCCCAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTTTGCTCAACCTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTATGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTAGCTAATTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCTAAGCGGATGAAAGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTGTGAAGGTTGATACCGAGGGAAATGGGGTGGAGTTTTTTTCTGGGACTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAATCTACGAATGTAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGGGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATAGATTTACACAAAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTGTTACGCAGGAGAAGTTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAATTTGCTGAAGCCAGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGATTCTTTAAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGAGGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCATCTAATACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGTGTTCTTTTAGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAAGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAAACCAAAACCCACATCTTGA

Protein sequence

MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKALAHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGDIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS

Homology

[Close]

BLAST of PI0012409 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 4.9e-279
Identity = 472/640 (73.75%), Postives = 535/640 (83.59%), Query Frame = 0


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRNPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL +   K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  L  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVVKVDT--EGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLHK MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE+++AY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

[Close]

BLAST of PI0012409 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 5.0e-255
Identity = 436/641 (68.02%), Postives = 508/641 (79.25%), Query Frame = 0


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLS--SRNPKLFFISQTGTSKALA 63
           +S ++ L +L LLS+S+++  YKS RLR+      P     +  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
             L   L   G+     D   ++P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGD 183
            DFR G+ LLS  +F+VFGVGSR YGETFNA AR FS+ ++ALGA E++AVGEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDI----V 243
           ++  F++W  ++++VVK      G E   G  G+SD     E+EE DDD  EE++    V
Sbjct: 192 LEVVFEEWCGRVVRVVK------GEEIGEGHNGESDGFDELEEEESDDDDDEEEVDGGEV 251

Query: 244 DLEDIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 303
           D+EDIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKIC
Sbjct: 252 DMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKIC 311

Query: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 363
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDD
Sbjct: 312 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDD 371

Query: 364 PLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 423
           PLDIVN+AID H KM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DEL
Sbjct: 372 PLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDEL 431

Query: 424 HRRRISTFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 483
           HRR ISTFLVTNAQFPDKI  LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSLK
Sbjct: 432 HRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLK 491

Query: 484 ALNEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENV 543
           +L++K QRTVYRLTLVKGWN E+I+ Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 492 SLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENV 551

Query: 544 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLV 603
           PWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF+++G+W TWIDY++FH+LV
Sbjct: 552 PWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELV 611

Query: 604 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 635
            SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 TSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646

[Close]

BLAST of PI0012409 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 1.1e-177
Identity = 324/651 (49.77%), Postives = 429/651 (65.90%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNLLNSNGLVFDLVDPKDYEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +   GL  +L++ K+Y+P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRM 166
            DG PP  A++   WL E++ DFR G   L   +++VFG+G+ VY   +N V ++  K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRKLLKVVKV-------DTEGNGVEFF 226
             L A  ++   EGD +      G I+  F+ W RK L  +K           G   +  
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGK 263

Query: 227 SGTAGDSDAESVEEDEEEDDDLGEED------------------------IVDLEDIAGK 286
             +   S  ESVEE+EEE++   E +                        ++D+ED+ GK
Sbjct: 264 CKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-GK 323

Query: 287 GPS--RKSTNVVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSGV 346
             S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSGV
Sbjct: 324 AMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGV 383

Query: 347 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWE 406
           K+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+
Sbjct: 384 KLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWK 443

Query: 407 MDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLV 466
           MD P  I+  AI  H+ MIKQ KGVPGV  E+ EEGL+ +HCALSLVGEPIMYPEINT +
Sbjct: 444 MDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFL 503

Query: 467 DELHRRRISTFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFID 526
             LH + IS+FLVTNAQFP++I  L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFID
Sbjct: 504 RLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFID 563

Query: 527 SLKALNEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTM 586
           SLKAL EKQQRTVYRLTLVK WN ++++AY++L ++G PDF+E+KGVTYCG S+ S LTM
Sbjct: 564 SLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLTM 623

Query: 587 ENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFH 636
            NVPWH +V  F E L      +YEVACEH HS C+L+A   KF+++G+W TWIDYE+F 
Sbjct: 624 ANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLIAH-KKFKINGEWCTWIDYERFQ 683

[Close]

BLAST of PI0012409 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 4.3e-174
Identity = 311/648 (47.99%), Postives = 421/648 (64.97%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNLLNSNGLVFDLVDPKDYEPEDLFKE-----TLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + G+  +++D KD++PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+ VY   FN V++   K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDTEG------------- 226
             L A  I+  GEGD +      G +   F  W  K L  ++   +G             
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKAS 259

Query: 227 --------NGVEFFSGTAGDSDAE------SVEEDEEEDDDLGEEDIVDLEDIAG----- 286
                      E     A  +++E      S +E+   +D+     ++D+ED+       
Sbjct: 260 CKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHM 319

Query: 287 -KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKIC 346
            K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+ 
Sbjct: 320 KKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLR 379

Query: 347 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 406
           RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD 
Sbjct: 380 RWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDP 439

Query: 407 PLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 466
              I+  A++ H+ MI+Q +GVPGV  E+ EEGL+ +HCALSLVGEPIMYPEIN+ +  L
Sbjct: 440 AEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLL 499

Query: 467 HRRRISTFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 526
           H++ IS+FLVTNAQFP++I  L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL+
Sbjct: 500 HQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLR 559

Query: 527 ALNEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENV 586
           AL EKQQRTVYRLTLVK WN ++++AY++L ++G+PDF+E+KGVTYCG S+ S LTM NV
Sbjct: 560 ALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMANV 619

Query: 587 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLV 636
           PWH +V  F + L A    +YE+ACEH HS C+LLA   KF+VDG+W+TWIDYE+F +L+
Sbjct: 620 PWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQELI 679

[Close]

BLAST of PI0012409 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 1.8e-172
Identity = 309/650 (47.54%), Postives = 413/650 (63.54%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNLLNSNGLVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S  L   +++ K+Y+P+D     +  + + +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+  Y   FN V ++  K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDS 226
             LGA  +++ GEGD D      G I+  F  W  K +  ++   +G   +   G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 DAESVEEDEEE--------------------------DDDLGEED------IVDLEDIAG 286
             ES +   EE                          +++ G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H+ MIKQ KGVPGV  E+ EEG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH+ +IS+FLVTNAQFP +I  L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

Query: 527 LKALNEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
           LKAL  KQQRTVYRLTLVK WN ++++AY+ L S+G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619

Query: 587 NVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHD 636
           +VPWH +V  F   L      EYE+ACEH HS C+L+A   KF++ G+W+TWIDY +F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679

[Close]

BLAST of PI0012409 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/641 (97.35%), Postives = 630/641 (98.28%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVD EGNGVE  SG AGDSDA SVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of PI0012409 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 623/641 (97.19%), Postives = 631/641 (98.44%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVD EGNGVE  SG AGDSDA SVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of PI0012409 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 619/640 (96.72%), Postives = 627/640 (97.97%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SR PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKV+K D EGNGVE  SG AGDSDA SVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of PI0012409 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 600/640 (93.75%), Postives = 616/640 (96.25%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+KVD  G G + +SG   D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI  LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

[Close]

BLAST of PI0012409 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 597/640 (93.28%), Postives = 616/640 (96.25%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSN L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+KVD  G G + +SG   D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI  LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR+DGQWFTWIDYEKFH LVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

[Close]

BLAST of PI0012409 vs. NCBI nr
Match: KAA0065238.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/641 (97.35%), Postives = 630/641 (98.28%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVD EGNGVE  SG AGDSDA SVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of PI0012409 vs. NCBI nr
Match: XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 623/641 (97.19%), Postives = 631/641 (98.44%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVD EGNGVE  SG AGDSDA SVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of PI0012409 vs. NCBI nr
Match: XP_004152698.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_021988 [Cucumis sativus])

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 619/640 (96.72%), Postives = 627/640 (97.97%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SR PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKV+K D EGNGVE  SG AGDSDA SVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of PI0012409 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 613/640 (95.78%), Postives = 629/640 (98.28%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTLSSR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGETFNAVAR+FSKRMKALGAKEILAVG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DID+CFDDWSR+LLKV+KVD  GNGV+ +SG  GDSDAESVEEDEEED DLGEEDIVDLE
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI LLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 640

[Close]

BLAST of PI0012409 vs. NCBI nr
Match: XP_023546392.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 600/640 (93.75%), Postives = 618/640 (96.56%), Query Frame = 0


Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRNPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLN+LNSN L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDTEGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+KVD  G G + +SG   D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADSGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIST
Sbjct: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIST 420

Query: 421 FLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKI  LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDI+AYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

[Close]

BLAST of PI0012409 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 961.4 bits (2484), Expect = 3.5e-280
Identity = 472/640 (73.75%), Postives = 535/640 (83.59%), Query Frame = 0


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRNPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL +   K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNLLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  L  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVVKVDT--EGNGVEFFSGTAGDSDAESVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLHK MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKINLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIEAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE+++AY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

The following BLAST results are available for this feature:

BLAST of PI0012409 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXN5	4.9e-279	73.75	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
Q8H8N3	5.0e-255	68.02	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
Q2KHP8	1.1e-177	49.77	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopu...	[more]
Q08C92	4.3e-174	47.99	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio ...	[more]
Q9NV66	1.8e-172	47.54	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo s...	[more]

BLAST of PI0012409 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VC83	0.0e+00	97.35	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BAY2	0.0e+00	97.19	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LPA4	0.0e+00	96.72	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN...	[more]
A0A6J1K9S7	0.0e+00	93.75	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HDZ5	0.0e+00	93.28	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662...	[more]

BLAST of PI0012409 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065238.1	0.0e+00	97.35	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo ...	[more]
XP_008444711.1	0.0e+00	97.19	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
XP_004152698.1	0.0e+00	96.72	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativ...	[more]
XP_038884961.1	0.0e+00	95.78	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa his...	[more]
XP_023546392.1	0.0e+00	93.75	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pep...	[more]

BLAST of PI0012409 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75200.1	3.5e-280	73.75	flavodoxin family protein / radical SAM domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 95..106 score: 39.97 coord: 133..143 score: 42.76 coord: 47..60 score: 35.6 coord: 158..177 score: 33.36
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 e-value: 3.0E-25 score: 89.1
IPR008254	Flavodoxin/nitric oxide synthase	PROSITE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 22.446825
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 324..502 e-value: 2.7E-25 score: 89.7
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 312..555 score: 14.566998
IPR013917	tRNA wybutosine-synthesis	PFAM	PF08608	Wyosine_form	coord: 507..570 e-value: 2.4E-26 score: 91.9
IPR023993	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, archaea	TIGRFAM	TIGR03972	TIGR03972	coord: 274..568 e-value: 1.0E-95 score: 318.2
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 251..582 e-value: 5.1E-154 score: 514.3
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 26..205 e-value: 3.2E-39 score: 136.2
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 45..198
None	No IPR available	SFLD	SFLDG01071	tRNA_wybutosine-synthesizing	coord: 263..575 e-value: 0.0 score: 490.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 217..233
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 208..233
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 611..640
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..635
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 324..496 e-value: 3.63965E-7 score: 49.2542
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 325..504
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	SFLD	SFLDF00284	tRNA_wybutosine-synthesizing	coord: 263..575 e-value: 0.0 score: 490.4
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..635

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0012409.3	PI0012409.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0031591	wybutosine biosynthetic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0102521	tRNA-4-demethylwyosine synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0016829	lyase activity