PI0012355 (gene) Melon (PI 482460) v1

BLAST of PI0012355 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.4e-39
Identity = 127/389 (32.65%), Postives = 206/389 (52.96%), Query Frame = 0

Query: 6   RYKLGLLLILSVVIMWVSSAEVTQSIFAD--YEQPFAISYVTTSLWIVYL-PIALLKDWL 65
           R+ LGLL++  V+I+WV S+ +   IF D  Y +PF I+Y  T+ +I YL P A      
Sbjct: 12  RWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPTA------ 71

Query: 66  LSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPLV 125
                           V  +  +  +  V+    +E +G  S  N ++D+ S        
Sbjct: 72  --------------KAVVVNYKDTGRANVHRELIMEEEGTGSDSNRSVDMTSPLLTNLEA 131

Query: 126 AVHIG--KENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 185
             H    K  TL +  K +A+      FC+  +WF    +TNA+LA TSVAS T+LS+TS
Sbjct: 132 GTHANQKKRLTLYETIKLSAE------FCI--LWFTANLVTNASLAFTSVASQTILSTTS 191

Query: 186 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQK---NANGNENHA- 245
             FTL IGA+   ++++  KV+   +S  G+ M T  K+ +    Q+   + +G++N A 
Sbjct: 192 SFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVT--KSDSHQRYQRHIADVSGDDNDAV 251

Query: 246 --LLGNVFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAM 305
             L+GN+ ++  +V YG+++ LLK+  G    ++M+  FG +GLF  + LW  +  L   
Sbjct: 252 QVLIGNLLALAGAVLYGVYSTLLKREVGDETRVNMKIFFGFVGLFNLLFLWPSLIVLDFF 311

Query: 306 GIEPRFTFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLED 365
           G EP F+ P+   V  +I VN  + +F+SD+ WA A++ TSPL   +G+S+TIP+AM  D
Sbjct: 312 GWEP-FSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGD 368

Query: 366 MVIHGRQYSVIYIIGSAQVFFGFVIANLS 384
           ++   +  S +Y+ G+  +   F I N S
Sbjct: 372 VIFKHKTMSALYLFGATLILGSFFIINKS 368

BLAST of PI0012355 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.6e-33
Identity = 114/419 (27.21%), Postives = 195/419 (46.54%), Query Frame = 0

Query: 6   RYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVY-LPIALLKDWLLS 65
           R  LG++++L V ++WV+S+E+T  +F  Y +PF  ++  TS++++Y L   + K W   
Sbjct: 68  RMALGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIVWKPWRQQ 127

Query: 66  FLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCK----------NCTIDVYS 125
             R +  K        +        +  + S L     +  K          N  ID   
Sbjct: 128 CTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEK 187

Query: 126 KDEGTPLVAVHI-------------------------GKENTLKKDRKFTAKEVAAFGFC 185
             + + +   +I                          +E+ LK   K TA +VA   F 
Sbjct: 188 IPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKTVGKLTATQVAKISFF 247

Query: 186 VAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALL---GEDTINIIKVVSVVV 245
              +WFL  +    AL+ T VA   +LSSTSGLFTL++ A+      D   + K+++V++
Sbjct: 248 FCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVIL 307

Query: 246 SMAGVAMTTFGKTSAADELQKNANGNE---NHALLGNVFSVLSSVTYGLFTVLLKKFAGG 305
           S+ GV +              N +G+E       +G+++S++ ++ Y ++ V++K+    
Sbjct: 308 SIGGVVLV-------------NLSGSEKSPGRNTIGSIWSLVGAMLYAVYIVMIKRKVDR 367

Query: 306 GQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFPRSANVEEVILVNAFVGSFLS 365
              LD+   FG +GLF  + LW   + L   G E  F FP    V   I++N  +G+ LS
Sbjct: 368 EDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFED-FEFPNKV-VLMCIVINGLIGTVLS 427

Query: 366 DYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSVIYIIGSAQVFFGFVIANL 383
           ++ W      TS L+  L +SLTIP++++ DM +   Q+S ++  G+  VFF F IA L
Sbjct: 428 EFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIATL 471

BLAST of PI0012355 vs. ExPASy Swiss-Prot
Match: Q8R314 (Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.7e-33
Identity = 115/425 (27.06%), Postives = 193/425 (45.41%), Query Frame = 0

Query: 6   RYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVY-LPIALLKDWLLS 65
           R  LG++++L V ++WV+S+E+T  +F  Y +PF  ++  TS++++Y L   + K W   
Sbjct: 68  RMALGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQ 127

Query: 66  FLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPLVAV 125
             R +  K        +        + +++S L     +  K      +       L + 
Sbjct: 128 CTRGFRGKPAAFFADAEGYFAACTTDTSMSSSLSEPLYVPVK------FHDLPSEKLEST 187

Query: 126 HIGKENTLKKDR-----------------------------------------KFTAKEV 185
           +IG E T KK R                                         K TA +V
Sbjct: 188 NIGTEKTPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKDQESILKTVGKLTATQV 247

Query: 186 AAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALL---GEDTINIIK 245
           A   F    +WFL       AL+ T VA   +LSSTSGLFTL++ A+      D   + K
Sbjct: 248 AKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSK 307

Query: 246 VVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHA---LLGNVFSVLSSVTYGLFTVLL 305
           +++V++S+ GV +              N +G+E  A    +G+++S+  ++ Y ++ V++
Sbjct: 308 LLAVILSIGGVVLV-------------NLSGSEKSAGRDTIGSIWSLAGAMFYAVYIVMI 367

Query: 306 KKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFPRSANVEEVILVNAF 365
           K+       LD+   FG +GLF  + LW   + L   G E  F FP    V   I++N  
Sbjct: 368 KRKVDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFED-FEFPNKV-VLLCIIINGL 427

Query: 366 VGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSVIYIIGSAQVFFGF 383
           +G+ LS++ W      TS L+  L +SLTIP++++ DM +   Q+S ++  G+  VFF F
Sbjct: 428 IGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSF 471

BLAST of PI0012355 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.2e-32
Identity = 114/418 (27.27%), Postives = 191/418 (45.69%), Query Frame = 0

Query: 6   RYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVY-LPIALLKDWLLS 65
           R  LG++++L V ++WV+S+E+T  +F  Y +PF  ++  TS++++Y L   + K W   
Sbjct: 68  RMALGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQ 127

Query: 66  FLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEH---------------------QGEL 125
             R    K        +        +  + S L                         E 
Sbjct: 128 CTRGLRGKHAAFFADAEGYFAACATDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEK 187

Query: 126 SCKNCTIDVYSKDEGTPLVAVHI-------------GKENTLKKDRKFTAKEVAAFGFCV 185
           + K   +   +  E   L + H               +E+ LK   K TA +VA   F  
Sbjct: 188 TPKKSRVRFSNIMEIRQLPSNHALESKLSRMSYPVKEQESILKTVGKLTATQVAKISFFF 247

Query: 186 APIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALL---GEDTINIIKVVSVVVS 245
             +WFL       AL+ T VA   +LSSTSGLFTL++ A+      D   + K+++V++S
Sbjct: 248 CFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVILS 307

Query: 246 MAGVAMTTFGKTSAADELQKNANGNENHA---LLGNVFSVLSSVTYGLFTVLLKKFAGGG 305
           + GV +              N +G+E  A    +G+++S+  ++ Y ++ V++K+     
Sbjct: 308 IGGVVLV-------------NLSGSEKSAGRDTIGSIWSLAGAMLYAVYIVMIKRKVDRE 367

Query: 306 QNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFPRSANVEEVILVNAFVGSFLSD 365
             LD+   FG +GLF  + LW   + L   G E  F FP    V   I++N  +G+ LS+
Sbjct: 368 DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFED-FEFPNKV-VLMCIIINGLIGTVLSE 427

Query: 366 YFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSVIYIIGSAQVFFGFVIANL 383
           + W      TS L+  L +SLTIP++++ DM +   Q+S ++  G+  VFF F I  L
Sbjct: 428 FLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIVTL 470

BLAST of PI0012355 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.2e-32
Identity = 106/378 (28.04%), Postives = 183/378 (48.41%), Query Frame = 0

Query: 7   YKLGLLLILSVVIMWVSSAEVTQSIFAD--YEQPFAISYVTTSLWIVYLPIALLKDWLLS 66
           + +G+LL+  VV+ WV ++ +T  +     Y +PF ++Y+  S + +YL   L   W   
Sbjct: 10  HMIGVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLTPDL---W--- 69

Query: 67  FLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPLVAV 126
             R   S+R  L    + ++ +   E                       S  E  PL++ 
Sbjct: 70  --RIIQSRRKSLQERTERTLPIHTQE-----------------------SFSEFLPLLSS 129

Query: 127 HIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFT 186
                + L        K+          +WF+     NAAL+ T+VAS+T+LSSTS  FT
Sbjct: 130 TPSTSSNLSSIADTKVKDTMRLSLLFCVLWFVANLAANAALSYTTVASSTILSSTSSFFT 189

Query: 187 LLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGNVFSV 246
           L +   LG +T +  K++ + VS+ G+ +     +   D +  ++       L+GN  ++
Sbjct: 190 LFLATSLGIETFSTKKLLGLFVSLFGIILIVMQSSKQQDSVSASS------FLVGNTLAL 249

Query: 247 LSSVTYGLFTVLLK-KFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFP 306
           L S+ Y ++T LLK + +  G  LD+Q   G +G+FTF+  W ++  L    +E  F  P
Sbjct: 250 LGSLGYSVYTTLLKYEISSKGLRLDIQMFLGYVGIFTFLLFWPILIILDITHME-TFELP 309

Query: 307 RSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYS 366
            + ++  ++++N  +  F+SDYFW  A++ TSPLV  + ++ TIP+AM  D V     ++
Sbjct: 310 SNFHISFLVMLNCII-IFVSDYFWCKALILTSPLVVTVALTFTIPLAMFADFVWREAFFT 348

Query: 367 VIYIIGSAQVFFGFVIAN 382
             YIIG   +F  F + N
Sbjct: 370 PWYIIGVIFIFVSFFLVN 348

BLAST of PI0012355 vs. ExPASy TrEMBL
Match: A0A0A0LVW6 (EamA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G222870 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.9e-219
Identity = 401/409 (98.04%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD
Sbjct: 17  MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 76

Query: 61  WLLSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTP 120
           WLLSFL RYNSKRGDLSVVGQSSVELQKNEVNIASELE QGELSCKNCT+DVYSKDEGTP
Sbjct: 77  WLLSFLHRYNSKRGDLSVVGQSSVELQKNEVNIASELEPQGELSCKNCTVDVYSKDEGTP 136

Query: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180
           LVAVHIGKE TLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS
Sbjct: 137 LVAVHIGKETTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 196

Query: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 240
           GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN
Sbjct: 197 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 256

Query: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300
           VFSVLSSVTYGLFTVLLKKFAGGGQNLD+QKLFGCIGLFTFVALWWLVWPLTAMGIEPRF
Sbjct: 257 VFSVLSSVTYGLFTVLLKKFAGGGQNLDLQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 316

Query: 301 TFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 360
           TFP SANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIP+AMLEDMVIHGR
Sbjct: 317 TFPHSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPIAMLEDMVIHGR 376

Query: 361 QYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           QYSVIYIIGSAQVF GFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL
Sbjct: 377 QYSVIYIIGSAQVFLGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 425

BLAST of PI0012355 vs. ExPASy TrEMBL
Match: A0A1S3B991 (uncharacterized transporter C405.03c-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487403 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 396/409 (96.82%), Postives = 399/409 (97.56%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVI+WVSSAEVTQSIF DYEQPFAISYVTTSLWIVYLPIALLKD
Sbjct: 1   MKMCWRYKLGLLLILSVVILWVSSAEVTQSIFEDYEQPFAISYVTTSLWIVYLPIALLKD 60

Query: 61  WLLSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTP 120
           WLLSFL RYNSKRGDL VVGQSSVELQKNEVNIASELEHQ ELSCKNCTIDVYSKDEG P
Sbjct: 61  WLLSFLHRYNSKRGDLCVVGQSSVELQKNEVNIASELEHQRELSCKNCTIDVYSKDEGAP 120

Query: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180
           LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS
Sbjct: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180

Query: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 240
           GLFTLLIGALLGEDTINIIKVVSVVVSMAGV MTTFGKTSAADELQKNANGNENHALLGN
Sbjct: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVVMTTFGKTSAADELQKNANGNENHALLGN 240

Query: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300
           VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF
Sbjct: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300

Query: 301 TFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 360
           TFP SANVEEVI+VNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIP AMLEDMVIHGR
Sbjct: 301 TFPHSANVEEVIIVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPFAMLEDMVIHGR 360

Query: 361 QYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           QYSVIYIIGS QVF GFVIANLSDWFSQNLASKV+RTTSQLQSLSFGPL
Sbjct: 361 QYSVIYIIGSVQVFLGFVIANLSDWFSQNLASKVARTTSQLQSLSFGPL 409

BLAST of PI0012355 vs. ExPASy TrEMBL
Match: A0A6J1L0I2 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499930 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 6.1e-201
Identity = 369/408 (90.44%), Postives = 389/408 (95.34%), Query Frame = 0

Query: 3   MCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL 62
           MCWRYKLGLLLILSVV+MWVSSAEVTQSIFADYEQPFA+SYVTTSL IVYLPIA LKDWL
Sbjct: 1   MCWRYKLGLLLILSVVVMWVSSAEVTQSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWL 60

Query: 63  LSFLRRYNSKRGD-LSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPL 122
           +SF    NSK GD L+VV QSSVELQK EV+ ASE+EHQG+LSCKNCTID+YSKDEGTPL
Sbjct: 61  MSFFYCCNSKCGDNLNVVYQSSVELQKVEVSFASEIEHQGQLSCKNCTIDMYSKDEGTPL 120

Query: 123 VAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSG 182
           VAVHI KENTLK+DRKFTAKEVAAFGFC+APIWFLTEYLTNAALARTSVASTTLLSSTSG
Sbjct: 121 VAVHIDKENTLKRDRKFTAKEVAAFGFCIAPIWFLTEYLTNAALARTSVASTTLLSSTSG 180

Query: 183 LFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGNV 242
           LFTLLIGALLGEDTINI+KVVSVVVSMAGVAMTTFGKTSAADELQKN NG EN+ALLGNV
Sbjct: 181 LFTLLIGALLGEDTINIVKVVSVVVSMAGVAMTTFGKTSAADELQKNGNGIENNALLGNV 240

Query: 243 FSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFT 302
           FSVLSS+TYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF 
Sbjct: 241 FSVLSSMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFL 300

Query: 303 FPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQ 362
           FP SANVE+VILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQ
Sbjct: 301 FPHSANVEKVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQ 360

Query: 363 YSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           YS IYIIGSAQVF GFV+ANLSDWFSQNLASK+SRTTSQLQS+SF P+
Sbjct: 361 YSAIYIIGSAQVFLGFVVANLSDWFSQNLASKLSRTTSQLQSISFWPM 408

BLAST of PI0012355 vs. ExPASy TrEMBL
Match: A0A6J1F619 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita moschata OX=3662 GN=LOC111441155 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.0e-200
Identity = 368/408 (90.20%), Postives = 388/408 (95.10%), Query Frame = 0

Query: 3   MCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL 62
           MCWRYKLGLLLILSVV+ WVSSAEVTQSIFADYEQPFA+SYVTTSL IVYLPIA LKDWL
Sbjct: 1   MCWRYKLGLLLILSVVVTWVSSAEVTQSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWL 60

Query: 63  LSFLRRYNSKRGD-LSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPL 122
           +SF    NSK GD L+VV QSSVELQK EVN ASE+EHQG+LSCKNCTID+YSKDEGTPL
Sbjct: 61  MSFFYCCNSKCGDNLNVVYQSSVELQKVEVNFASEIEHQGQLSCKNCTIDMYSKDEGTPL 120

Query: 123 VAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSG 182
           VAVHI KENTLK+DRKFTAKEVAAFGFC+APIWFLTEYLTNAALARTSVASTTLLSSTSG
Sbjct: 121 VAVHIDKENTLKRDRKFTAKEVAAFGFCIAPIWFLTEYLTNAALARTSVASTTLLSSTSG 180

Query: 183 LFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGNV 242
           LFTLLIGALLGEDTINI+KVVSVVVSMAGVAMTTFGKTSAADELQKN NG EN+ALLGNV
Sbjct: 181 LFTLLIGALLGEDTINIVKVVSVVVSMAGVAMTTFGKTSAADELQKNGNGIENNALLGNV 240

Query: 243 FSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFT 302
           FSVLSS+TYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF 
Sbjct: 241 FSVLSSMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFV 300

Query: 303 FPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQ 362
           FP SANVE+VILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQ
Sbjct: 301 FPHSANVEKVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQ 360

Query: 363 YSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           YS IYIIGSAQVF GFV+AN+SDWFSQNLASK+SRTTSQLQS+SF P+
Sbjct: 361 YSAIYIIGSAQVFLGFVVANISDWFSQNLASKLSRTTSQLQSISFWPM 408

BLAST of PI0012355 vs. ExPASy TrEMBL
Match: A0A1S3BA01 (uncharacterized transporter C405.03c-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487403 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.7e-200
Identity = 370/409 (90.46%), Postives = 373/409 (91.20%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVI+WVSSAEVTQSIF DYEQPFAISYVTTSLWIVYLPIALLKD
Sbjct: 1   MKMCWRYKLGLLLILSVVILWVSSAEVTQSIFEDYEQPFAISYVTTSLWIVYLPIALLKD 60

Query: 61  WLLSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTP 120
           WLLSFL RYNSKRGDL VVGQSSVELQKNEVNIASELEHQ ELSCKNCTIDVYSKDEG P
Sbjct: 61  WLLSFLHRYNSKRGDLCVVGQSSVELQKNEVNIASELEHQRELSCKNCTIDVYSKDEGAP 120

Query: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180
           LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS
Sbjct: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180

Query: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 240
           G                          MAGV MTTFGKTSAADELQKNANGNENHALLGN
Sbjct: 181 G--------------------------MAGVVMTTFGKTSAADELQKNANGNENHALLGN 240

Query: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300
           VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF
Sbjct: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300

Query: 301 TFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 360
           TFP SANVEEVI+VNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIP AMLEDMVIHGR
Sbjct: 301 TFPHSANVEEVIIVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPFAMLEDMVIHGR 360

Query: 361 QYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           QYSVIYIIGS QVF GFVIANLSDWFSQNLASKV+RTTSQLQSLSFGPL
Sbjct: 361 QYSVIYIIGSVQVFLGFVIANLSDWFSQNLASKVARTTSQLQSLSFGPL 383

BLAST of PI0012355 vs. NCBI nr
Match: XP_004150512.1 (uncharacterized vacuolar membrane protein YML018C [Cucumis sativus] >XP_011655305.1 uncharacterized vacuolar membrane protein YML018C [Cucumis sativus] >KGN65104.1 hypothetical protein Csa_004556 [Cucumis sativus])

HSP 1 Score: 770.8 bits (1989), Expect = 6.0e-219
Identity = 401/409 (98.04%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD
Sbjct: 17  MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 76

Query: 61  WLLSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTP 120
           WLLSFL RYNSKRGDLSVVGQSSVELQKNEVNIASELE QGELSCKNCT+DVYSKDEGTP
Sbjct: 77  WLLSFLHRYNSKRGDLSVVGQSSVELQKNEVNIASELEPQGELSCKNCTVDVYSKDEGTP 136

Query: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180
           LVAVHIGKE TLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS
Sbjct: 137 LVAVHIGKETTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 196

Query: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 240
           GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN
Sbjct: 197 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 256

Query: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300
           VFSVLSSVTYGLFTVLLKKFAGGGQNLD+QKLFGCIGLFTFVALWWLVWPLTAMGIEPRF
Sbjct: 257 VFSVLSSVTYGLFTVLLKKFAGGGQNLDLQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 316

Query: 301 TFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 360
           TFP SANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIP+AMLEDMVIHGR
Sbjct: 317 TFPHSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPIAMLEDMVIHGR 376

Query: 361 QYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           QYSVIYIIGSAQVF GFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL
Sbjct: 377 QYSVIYIIGSAQVFLGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 425

BLAST of PI0012355 vs. NCBI nr
Match: XP_008443928.1 (PREDICTED: uncharacterized transporter C405.03c-like isoform X1 [Cucumis melo] >XP_008443929.1 PREDICTED: uncharacterized transporter C405.03c-like isoform X1 [Cucumis melo])

HSP 1 Score: 761.9 bits (1966), Expect = 2.8e-216
Identity = 396/409 (96.82%), Postives = 399/409 (97.56%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVI+WVSSAEVTQSIF DYEQPFAISYVTTSLWIVYLPIALLKD
Sbjct: 1   MKMCWRYKLGLLLILSVVILWVSSAEVTQSIFEDYEQPFAISYVTTSLWIVYLPIALLKD 60

Query: 61  WLLSFLRRYNSKRGDLSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTP 120
           WLLSFL RYNSKRGDL VVGQSSVELQKNEVNIASELEHQ ELSCKNCTIDVYSKDEG P
Sbjct: 61  WLLSFLHRYNSKRGDLCVVGQSSVELQKNEVNIASELEHQRELSCKNCTIDVYSKDEGAP 120

Query: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180
           LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS
Sbjct: 121 LVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTS 180

Query: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGN 240
           GLFTLLIGALLGEDTINIIKVVSVVVSMAGV MTTFGKTSAADELQKNANGNENHALLGN
Sbjct: 181 GLFTLLIGALLGEDTINIIKVVSVVVSMAGVVMTTFGKTSAADELQKNANGNENHALLGN 240

Query: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300
           VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF
Sbjct: 241 VFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF 300

Query: 301 TFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 360
           TFP SANVEEVI+VNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIP AMLEDMVIHGR
Sbjct: 301 TFPHSANVEEVIIVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPFAMLEDMVIHGR 360

Query: 361 QYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           QYSVIYIIGS QVF GFVIANLSDWFSQNLASKV+RTTSQLQSLSFGPL
Sbjct: 361 QYSVIYIIGSVQVFLGFVIANLSDWFSQNLASKVARTTSQLQSLSFGPL 409

BLAST of PI0012355 vs. NCBI nr
Match: XP_038880573.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida] >XP_038880574.1 uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida] >XP_038880575.1 uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 741.5 bits (1913), Expect = 3.9e-210
Identity = 390/410 (95.12%), Postives = 398/410 (97.07%), Query Frame = 0

Query: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKD 60
           MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSL IVYLPIALLKD
Sbjct: 1   MKMCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLLIVYLPIALLKD 60

Query: 61  WLLSFLRRYNSKRGD-LSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGT 120
           WLLSFL RYNSK  D  SVV +SS+ELQKNEVN+ASELEHQG+LSCKNCTIDVYSKDEGT
Sbjct: 61  WLLSFLYRYNSKSCDNPSVVDESSIELQKNEVNLASELEHQGQLSCKNCTIDVYSKDEGT 120

Query: 121 PLVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSST 180
           PLVAVHIGKENTLKKDR FTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSST
Sbjct: 121 PLVAVHIGKENTLKKDRMFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSST 180

Query: 181 SGLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLG 240
           SGLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNA+GNENHALLG
Sbjct: 181 SGLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNAHGNENHALLG 240

Query: 241 NVFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPR 300
           NVFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMG+EPR
Sbjct: 241 NVFSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGVEPR 300

Query: 301 FTFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHG 360
           FTFP+SANVE VIL NAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHG
Sbjct: 301 FTFPQSANVEGVILGNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHG 360

Query: 361 RQYSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           RQYSV+YIIGSAQVF GFVIANLSDWFSQNLASKVSRTTSQLQSL FGPL
Sbjct: 361 RQYSVVYIIGSAQVFLGFVIANLSDWFSQNLASKVSRTTSQLQSLFFGPL 410

BLAST of PI0012355 vs. NCBI nr
Match: XP_023007431.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 709.9 bits (1831), Expect = 1.3e-200
Identity = 369/408 (90.44%), Postives = 389/408 (95.34%), Query Frame = 0

Query: 3   MCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL 62
           MCWRYKLGLLLILSVV+MWVSSAEVTQSIFADYEQPFA+SYVTTSL IVYLPIA LKDWL
Sbjct: 1   MCWRYKLGLLLILSVVVMWVSSAEVTQSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWL 60

Query: 63  LSFLRRYNSKRGD-LSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPL 122
           +SF    NSK GD L+VV QSSVELQK EV+ ASE+EHQG+LSCKNCTID+YSKDEGTPL
Sbjct: 61  MSFFYCCNSKCGDNLNVVYQSSVELQKVEVSFASEIEHQGQLSCKNCTIDMYSKDEGTPL 120

Query: 123 VAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSG 182
           VAVHI KENTLK+DRKFTAKEVAAFGFC+APIWFLTEYLTNAALARTSVASTTLLSSTSG
Sbjct: 121 VAVHIDKENTLKRDRKFTAKEVAAFGFCIAPIWFLTEYLTNAALARTSVASTTLLSSTSG 180

Query: 183 LFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGNV 242
           LFTLLIGALLGEDTINI+KVVSVVVSMAGVAMTTFGKTSAADELQKN NG EN+ALLGNV
Sbjct: 181 LFTLLIGALLGEDTINIVKVVSVVVSMAGVAMTTFGKTSAADELQKNGNGIENNALLGNV 240

Query: 243 FSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFT 302
           FSVLSS+TYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF 
Sbjct: 241 FSVLSSMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFL 300

Query: 303 FPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQ 362
           FP SANVE+VILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQ
Sbjct: 301 FPHSANVEKVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQ 360

Query: 363 YSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           YS IYIIGSAQVF GFV+ANLSDWFSQNLASK+SRTTSQLQS+SF P+
Sbjct: 361 YSAIYIIGSAQVFLGFVVANLSDWFSQNLASKLSRTTSQLQSISFWPM 408

BLAST of PI0012355 vs. NCBI nr
Match: XP_023531838.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 709.1 bits (1829), Expect = 2.1e-200
Identity = 369/408 (90.44%), Postives = 388/408 (95.10%), Query Frame = 0

Query: 3   MCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL 62
           MCWRYKLGLLLILSVV+MWVSSAEVTQSIFADYEQPFA+SYVTTSL IVYLPIA LKDWL
Sbjct: 1   MCWRYKLGLLLILSVVVMWVSSAEVTQSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWL 60

Query: 63  LSFLRRYNSKRGD-LSVVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSKDEGTPL 122
           +SF    NSK GD L+VV QSSVELQK EVN ASE+EHQ +LSCKNCTID+YSKDEGTPL
Sbjct: 61  MSFFYCCNSKCGDNLNVVYQSSVELQKVEVNFASEIEHQEQLSCKNCTIDMYSKDEGTPL 120

Query: 123 VAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSSTSG 182
           VAVHI KENTLK+DRKFTAKEVAAFGFC+APIWFLTEYLTNAALARTSVASTTLLSSTSG
Sbjct: 121 VAVHIDKENTLKRDRKFTAKEVAAFGFCIAPIWFLTEYLTNAALARTSVASTTLLSSTSG 180

Query: 183 LFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLGNV 242
           LFTLLIGALLGEDTINI+KVVSVVVSMAGVAMTTFGKTSAADELQKN NG EN+ALLGNV
Sbjct: 181 LFTLLIGALLGEDTINIVKVVSVVVSMAGVAMTTFGKTSAADELQKNGNGIENNALLGNV 240

Query: 243 FSVLSSVTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFT 302
           FSVLSS+TYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF 
Sbjct: 241 FSVLSSMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFV 300

Query: 303 FPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQ 362
           FP SANVE+VILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQ
Sbjct: 301 FPHSANVEKVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQ 360

Query: 363 YSVIYIIGSAQVFFGFVIANLSDWFSQNLASKVSRTTSQLQSLSFGPL 410
           YS IYIIGSAQVF GFV+ANLSDWFSQNLASK+SRTTSQLQS+SF P+
Sbjct: 361 YSAIYIIGSAQVFLGFVVANLSDWFSQNLASKLSRTTSQLQSISFWPM 408

BLAST of PI0012355 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 439.1 bits (1128), Expect = 3.9e-123
Identity = 235/392 (59.95%), Postives = 301/392 (76.79%), Query Frame = 0

Query: 3   MCWRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL 62
           M WRYK GL LI +VVI+WV+SAEVTQ IF  Y+QPFA++Y+  SL IVYLP+A LKDWL
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 63  LSFLRRYNSKRGDL-SVVGQSSVELQKNEVNIASELEHQGELSCKNCT--IDVYSKDEGT 122
             +L R +SK   + ++   SSVEL     +   E+  QG ++ K+    +  + +++  
Sbjct: 61  CRYLDRRSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQGTITKKDSEEHLSSHEEEDER 120

Query: 123 PLVAVHIGKENTLKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAALARTSVASTTLLSST 182
           PL+     +  TLK+ ++ T K++A +G  +APIWF+TEYL+NAALARTSVASTT+LSST
Sbjct: 121 PLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTTVLSST 180

Query: 183 SGLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADELQKNANGNENHALLG 242
           SGLFTL IG  LG+DT+N+ KVV+V VSMAGV MTT GKT A+DE Q N++ N   +L+G
Sbjct: 181 SGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGERSLMG 240

Query: 243 NVFSVLSSVTYGLFTVLLKKFAG-GGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEP 302
           ++F +LS+V+YGLFTVLLKKFAG  G+ +D+QKLFG IGLFT VALWWLVWPLTA+GIEP
Sbjct: 241 DLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEP 300

Query: 303 RFTFPRSANVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIH 362
           +FT P S  V+EV+L N F+GS LSDYFWAL+VVWT+PLVA LG+SLTIP+AM+ DM+IH
Sbjct: 301 KFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVADMMIH 360

Query: 363 GRQYSVIYIIGSAQVFFGFVIANLSDWFSQNL 391
           GR YS IYI+GS QVF GFVIAN+SD FS+ L
Sbjct: 361 GRHYSAIYILGSTQVFAGFVIANISDLFSKKL 392

BLAST of PI0012355 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 122.5 bits (306), Expect = 8.0e-28
Identity = 108/403 (26.80%), Postives = 191/403 (47.39%), Query Frame = 0

Query: 5   WRYKLGLLLILSVVIMWVSSAEVTQSIFADYEQPFAISYVTTSLWIVYLPIALLKDWL-- 64
           WR+ LGL+ + +V  +W++++ V QS+      PF I+++  SL++VYLP+  +  +L  
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 65  -LSFLRRYNSKRGDLS--VVGQSSVELQKNEVNIASELEHQGELSCKNCTIDVYSK--DE 124
               L  + SKR  L   V  + +V L K+   + S+      L  +   I       + 
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNGIES 125

Query: 125 GTPLVAVHI-----------GKENT-LKKDRKFTAKEVAAFGFCVAPIWFLTEYLTNAAL 184
           G   + + I           G  N  L +  ++T   VA     + P WFL +   N +L
Sbjct: 126 GLENIELEIDASVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQLTFNVSL 185

Query: 185 ARTSVASTTLLSSTSGLFTLLIGALLGEDTINIIKVVSVVVSMAGVAMTTFGKTSAADEL 244
             T+V S T+LSS S LFT L+  +   +    +K+ SV++ M+G  + + G      + 
Sbjct: 186 KYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMG------DS 245

Query: 245 QKNANGNENHALLGNVFSVLSSVTYGLFTVLLKKFAGGGQ----NLDMQKLFGCIGLFTF 304
           +  +N    + LLG++ S++S+  Y ++  L++K           + M +  G +GLF F
Sbjct: 246 ESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGLFNF 305

Query: 305 VALWWLVWPLTAMGIEPRFTFPRSANVEEVILV--NAFVGSFLSDYFWALAVVWTSPLVA 364
              +  + P   +    R  F  +  +++  LV     + + LSDY WA AV+ T+  VA
Sbjct: 306 ---FIFLPPALILNFTKRERF-NALTLKQFGLVVGKGLLDNVLSDYLWAKAVLLTTTTVA 365

Query: 365 ALGVSLTIPVAMLEDMVIHGRQYSVIYIIGSAQVFFGFVIANL 383
             G+++ +P+A + D  + G + S    IG+A V  GF   N+
Sbjct: 366 TAGLTIQVPLAAIVDS-LSGNKPSFTDYIGAAAVMVGFAGINI 397