PI0012300 (gene) Melon (PI 482460) v1

Overview


Name	PI0012300
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	protein MIZU-KUSSEI 1
Location	chr08: 21003536 .. 21004204 (+)
RNA-Seq Expression	PI0012300
Synteny	PI0012300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTTACCCCGCCGTCAACGTCGCCGCCGTAGACTGCCAGAAACAAGTCCGTTCATGGCAGCTCTTCCGATCTCTAATTGAATTCCTCATTCCCACTTGTAACTGCGCCTTCACCGAAGATCAAGAACAACAACAACCCATGTATTATTCCTCAAAACCCATCTTCCCGCCGTCCTCCGCCGCCAATTCCGTCACCGGCACCATTTTCGGTTACCGTAGAGGAAAAGTCAACTTCTGTATCCAAACAAATTCCAGCTCAACCAACCCAATTCTCCTCCTCGAATTGGCGGTCCCCACCGCAATCCTCGCCCGGGAAATGAGAGGCGGCGTCCTCAGAATCGCCCTCGAATCCGCCACGGCGGCGAATTCAGGGGGGAGATCGGTTCTGTCGTCGCCGGCGTGGAGTATGTATTTCAATGGAAGGAAAGTTGGGTACGGATTAAGACGAGGGGCATCGGCGGCAGAGGTGGAGACGCTGCGGCGGTTGGGGAGGGTGGCGGAAGGGGCGGGAATTATTGAAGGTGAAGATGATTATTTGATGTATTTGAGAGGGAATTTTGACAGAGTTTGTGGAGCGTCGGGAGATTCTGAATCTTTCCATTTGAGAGATCCAAATGGAAGCATTGGTCAAGAGCTCAGTATTTTCTTTTTCCGATCCAAATAA

mRNA sequence

ATGGCCACTTACCCCGCCGTCAACGTCGCCGCCGTAGACTGCCAGAAACAAGTCCGTTCATGGCAGCTCTTCCGATCTCTAATTGAATTCCTCATTCCCACTTGTAACTGCGCCTTCACCGAAGATCAAGAACAACAACAACCCATGTATTATTCCTCAAAACCCATCTTCCCGCCGTCCTCCGCCGCCAATTCCGTCACCGGCACCATTTTCGGTTACCGTAGAGGAAAAGTCAACTTCTGTATCCAAACAAATTCCAGCTCAACCAACCCAATTCTCCTCCTCGAATTGGCGGTCCCCACCGCAATCCTCGCCCGGGAAATGAGAGGCGGCGTCCTCAGAATCGCCCTCGAATCCGCCACGGCGGCGAATTCAGGGGGGAGATCGGTTCTGTCGTCGCCGGCGTGGAGTATGTATTTCAATGGAAGGAAAGTTGGGTACGGATTAAGACGAGGGGCATCGGCGGCAGAGGTGGAGACGCTGCGGCGGTTGGGGAGGGTGGCGGAAGGGGCGGGAATTATTGAAGGTGAAGATGATTATTTGATGTATTTGAGAGGGAATTTTGACAGAGTTTGTGGAGCGTCGGGAGATTCTGAATCTTTCCATTTGAGAGATCCAAATGGAAGCATTGGTCAAGAGCTCAGTATTTTCTTTTTCCGATCCAAATAA

Coding sequence (CDS)

ATGGCCACTTACCCCGCCGTCAACGTCGCCGCCGTAGACTGCCAGAAACAAGTCCGTTCATGGCAGCTCTTCCGATCTCTAATTGAATTCCTCATTCCCACTTGTAACTGCGCCTTCACCGAAGATCAAGAACAACAACAACCCATGTATTATTCCTCAAAACCCATCTTCCCGCCGTCCTCCGCCGCCAATTCCGTCACCGGCACCATTTTCGGTTACCGTAGAGGAAAAGTCAACTTCTGTATCCAAACAAATTCCAGCTCAACCAACCCAATTCTCCTCCTCGAATTGGCGGTCCCCACCGCAATCCTCGCCCGGGAAATGAGAGGCGGCGTCCTCAGAATCGCCCTCGAATCCGCCACGGCGGCGAATTCAGGGGGGAGATCGGTTCTGTCGTCGCCGGCGTGGAGTATGTATTTCAATGGAAGGAAAGTTGGGTACGGATTAAGACGAGGGGCATCGGCGGCAGAGGTGGAGACGCTGCGGCGGTTGGGGAGGGTGGCGGAAGGGGCGGGAATTATTGAAGGTGAAGATGATTATTTGATGTATTTGAGAGGGAATTTTGACAGAGTTTGTGGAGCGTCGGGAGATTCTGAATCTTTCCATTTGAGAGATCCAAATGGAAGCATTGGTCAAGAGCTCAGTATTTTCTTTTTCCGATCCAAATAA

Protein sequence

MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPSSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK

Homology

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BLAST of PI0012300 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.1e-27
Identity = 75/177 (42.37%), Postives = 106/177 (59.89%), Query Frame = 0


Query: 60  SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALES 119
           SS    VTGT++G++RG V F +Q N  S +P+LLL+LA+ TA L +EM  G++RIALE 
Sbjct: 123 SSLGRRVTGTLYGHKRGHVTFSVQYNQRS-DPVLLLDLAMSTATLVKEMSSGLVRIALEC 182

Query: 120 ATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVE--TLRRLGRVAEGAGII--- 179
                SG + +   P W+MY NGRK GY + RG +  + +   L  + RV  GAG+I   
Sbjct: 183 EKRHRSGTK-LFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTP 242

Query: 180 EGEDDY-----------LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFR 221
           +  DD            L+Y+RG F+RV G S DSE+F++ +P+ + G ELSIF  R
Sbjct: 243 KTIDDVSGVGSGTELGELLYMRGKFERVVG-SRDSEAFYMMNPDKNGGPELSIFLLR 296

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BLAST of PI0012300 vs. ExPASy TrEMBL
Match: A0A5A7T4C8 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003070 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.1e-119
Identity = 218/222 (98.20%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPS 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP  
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPTP 60

Query: 61  SAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120
           SAANSVTGTIFGYRRGKVNFCIQT+SSSTNPILLLELAVPTAILAREMRGGVLRIALESA
Sbjct: 61  SAANSVTGTIFGYRRGKVNFCIQTSSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120

Query: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDY 180
           TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAG+IEGEDDY
Sbjct: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGVIEGEDDY 180

Query: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 222

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BLAST of PI0012300 vs. ExPASy TrEMBL
Match: A0A1S3B0Z8 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103484947 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.1e-119
Identity = 218/222 (98.20%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPS 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP  
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPTP 60

Query: 61  SAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120
           SAANSVTGTIFGYRRGKVNFCIQT+SSSTNPILLLELAVPTAILAREMRGGVLRIALESA
Sbjct: 61  SAANSVTGTIFGYRRGKVNFCIQTSSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120

Query: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDY 180
           TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAG+IEGEDDY
Sbjct: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGVIEGEDDY 180

Query: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 222

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BLAST of PI0012300 vs. ExPASy TrEMBL
Match: A0A0A0KG77 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G499200 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.7e-118
Identity = 216/222 (97.30%), Postives = 217/222 (97.75%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPS 60
           MATYP VNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPI PP 
Sbjct: 1   MATYPVVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPISPPP 60

Query: 61  SAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120
           S ANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA
Sbjct: 61  STANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120

Query: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDY 180
           T ANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAG+IEGEDDY
Sbjct: 121 TTANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGVIEGEDDY 180

Query: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 222

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BLAST of PI0012300 vs. ExPASy TrEMBL
Match: A0A6J1GES2 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453526 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.8e-99
Identity = 196/234 (83.76%), Postives = 204/234 (87.18%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP-P 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAF+E++       YSSKP FP  
Sbjct: 8   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFSEEEPT-----YSSKPTFPST 67

Query: 61  SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALES 120
           SS+A SVTGTIFGYRRGKVNFCIQT+S STNPILLLELAVPTAILAREMRGGVLRIALES
Sbjct: 68  SSSATSVTGTIFGYRRGKVNFCIQTHSKSTNPILLLELAVPTAILAREMRGGVLRIALES 127

Query: 121 ---ATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGII-- 180
              A A NSGGRSVLSSPAWSMYFNGRKVGY LRR ASAA+VETLRRLG + EGAGI+  
Sbjct: 128 TSTAAAVNSGGRSVLSSPAWSMYFNGRKVGYALRRRASAADVETLRRLGTMVEGAGIMGG 187

Query: 181 -------EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
                  EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS
Sbjct: 188 KEVEGEGEGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 236

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BLAST of PI0012300 vs. ExPASy TrEMBL
Match: A0A6J1ITF7 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478498 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.1e-98
Identity = 195/237 (82.28%), Postives = 203/237 (85.65%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP-P 60
           MATYPAVNVAAVDCQKQVRSWQ FRSLIEFLIPTCNCAF+E++       YSSKP FP  
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQFFRSLIEFLIPTCNCAFSEEEPT-----YSSKPTFPST 60

Query: 61  SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALES 120
           SS+A SVTGTIFGYRRGKVNFCIQT+S STNPILLLELAVPTAILAREMRGGVLRIALES
Sbjct: 61  SSSATSVTGTIFGYRRGKVNFCIQTHSKSTNPILLLELAVPTAILAREMRGGVLRIALES 120

Query: 121 ----ATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGII- 180
               A A NSGGRSVLSSPAWSMYFNGRKVGY LRR ASAA+VETLRRLG + EGAGI+ 
Sbjct: 121 TSTAAAAVNSGGRSVLSSPAWSMYFNGRKVGYALRRRASAADVETLRRLGTMVEGAGIMG 180

Query: 181 ----------EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
                     EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS
Sbjct: 181 GKEVEGEGEGEGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 232

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BLAST of PI0012300 vs. NCBI nr
Match: XP_008440568.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0036325.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK12719.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 437.2 bits (1123), Expect = 8.5e-119
Identity = 218/222 (98.20%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPS 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP  
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPTP 60

Query: 61  SAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120
           SAANSVTGTIFGYRRGKVNFCIQT+SSSTNPILLLELAVPTAILAREMRGGVLRIALESA
Sbjct: 61  SAANSVTGTIFGYRRGKVNFCIQTSSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120

Query: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDY 180
           TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAG+IEGEDDY
Sbjct: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGVIEGEDDY 180

Query: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 222

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BLAST of PI0012300 vs. NCBI nr
Match: XP_011657961.1 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN48725.1 hypothetical protein Csa_003562 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 5.5e-118
Identity = 216/222 (97.30%), Postives = 217/222 (97.75%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPS 60
           MATYP VNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPI PP 
Sbjct: 1   MATYPVVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPISPPP 60

Query: 61  SAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120
           S ANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA
Sbjct: 61  STANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALESA 120

Query: 121 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDY 180
           T ANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAG+IEGEDDY
Sbjct: 121 TTANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGVIEGEDDY 180

Query: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 LMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 222

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BLAST of PI0012300 vs. NCBI nr
Match: XP_038883078.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 400.6 bits (1028), Expect = 8.9e-108
Identity = 208/227 (91.63%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPP- 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAF+EDQEQQ    YSSKP+FPP 
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFSEDQEQQP--IYSSKPMFPPS 60

Query: 61  --SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIAL 120
             SSAANSVTGTIFGYRRGKVNFCIQT+S STNPILLLELAVPTAILAREM GGVLRIAL
Sbjct: 61  ISSSAANSVTGTIFGYRRGKVNFCIQTSSKSTNPILLLELAVPTAILAREMGGGVLRIAL 120

Query: 121 ESAT--AANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIE 180
           ES +  AANSGGRSVLSSPAWSMYFNGRKVGYGLRR ASAAEVETLRRLG VAEGAGI+E
Sbjct: 121 ESTSTAAANSGGRSVLSSPAWSMYFNGRKVGYGLRRRASAAEVETLRRLGTVAEGAGIME 180

Query: 181 GEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 223
           GEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK
Sbjct: 181 GEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRSK 225

[Close]

BLAST of PI0012300 vs. NCBI nr
Match: XP_022950422.1 (protein MIZU-KUSSEI 1-like [Cucurbita moschata])

HSP 1 Score: 370.5 bits (950), Expect = 9.8e-99
Identity = 196/234 (83.76%), Postives = 204/234 (87.18%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP-P 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAF+E++       YSSKP FP  
Sbjct: 8   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFSEEEPT-----YSSKPTFPST 67

Query: 61  SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALES 120
           SS+A SVTGTIFGYRRGKVNFCIQT+S STNPILLLELAVPTAILAREMRGGVLRIALES
Sbjct: 68  SSSATSVTGTIFGYRRGKVNFCIQTHSKSTNPILLLELAVPTAILAREMRGGVLRIALES 127

Query: 121 ---ATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGII-- 180
              A A NSGGRSVLSSPAWSMYFNGRKVGY LRR ASAA+VETLRRLG + EGAGI+  
Sbjct: 128 TSTAAAVNSGGRSVLSSPAWSMYFNGRKVGYALRRRASAADVETLRRLGTMVEGAGIMGG 187

Query: 181 -------EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
                  EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS
Sbjct: 188 KEVEGEGEGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 236

[Close]

BLAST of PI0012300 vs. NCBI nr
Match: XP_023544212.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo] >KAG6604051.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034217.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.5 bits (950), Expect = 9.8e-99
Identity = 196/234 (83.76%), Postives = 204/234 (87.18%), Query Frame = 0


Query: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFP-P 60
           MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAF+E++       YSSKP FP  
Sbjct: 1   MATYPAVNVAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFSEEEPT-----YSSKPTFPST 60

Query: 61  SSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIALES 120
           SS+A SVTGTIFGYRRGKVNFCIQT+S STNPILLLELAVPTAILAREMRGGVLRIALES
Sbjct: 61  SSSATSVTGTIFGYRRGKVNFCIQTHSKSTNPILLLELAVPTAILAREMRGGVLRIALES 120

Query: 121 ---ATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGII-- 180
              A A NSGGRSVLSSPAWSMYFNGRKVGY LRR ASAA+VETLRRLG + EGAGI+  
Sbjct: 121 TSTAAAVNSGGRSVLSSPAWSMYFNGRKVGYALRRRASAADVETLRRLGTMVEGAGIMGG 180

Query: 181 -------EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
                  EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS
Sbjct: 181 KEVEGEGEGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 229

[Close]

BLAST of PI0012300 vs. TAIR 10
Match: AT5G65340.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-56
Identity = 124/230 (53.91%), Postives = 158/230 (68.70%), Query Frame = 0


Query: 11  AVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPSSAANSV---- 70
           AVDC KQVRSW+L R++ + LIP+C C   +  + Q+    S + I P +S+A+S     
Sbjct: 23  AVDCHKQVRSWRLLRTIAQLLIPSCYCTIVDPNDSQEDK-LSHRQIKPRTSSASSTATNS 82

Query: 71  --TGTIFGYRRGKVNFCIQ-TNSSSTNPIL-LLELAVPTAILAREMRGGVLRIALESAT- 130
             TGTIFG+RRGKVNFCIQ TNS + NPI+ LLEL VPT +LAREM+GGVLRIALES   
Sbjct: 83  TFTGTIFGFRRGKVNFCIQATNSKTLNPIIVLLELTVPTEVLAREMQGGVLRIALESNNN 142

Query: 131 ----AANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGE 190
               +      S+L++P W+MY NGRKVG+ ++R  S +E+  L+ L  VAEGAG++ GE
Sbjct: 143 DGYDSHEDSSSSLLTTPLWNMYCNGRKVGFAIKREPSKSELAALKVLTPVAEGAGVVNGE 202

Query: 191 D------DYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
           +      D++MYLR +F RV G S DSESFHL DP G IGQELSIFF RS
Sbjct: 203 EINREKSDHMMYLRASFKRVFG-SFDSESFHLVDPRGIIGQELSIFFSRS 250

[Close]

BLAST of PI0012300 vs. TAIR 10
Match: AT2G22460.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 209.1 bits (531), Expect = 3.5e-54
Identity = 123/229 (53.71%), Postives = 147/229 (64.19%), Query Frame = 0


Query: 9   VAAVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQ------QPMYYSSK---PIFPP 68
           VA VDCQKQVRSW+L RS I+ LIPTCNC   ++ EQ       + +Y   K     F  
Sbjct: 17  VATVDCQKQVRSWRLLRSFIQLLIPTCNCTLVQELEQDNTNLRGKKLYNDIKSRTSSFRS 76

Query: 69  SSAANS--VTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMRGGVLRIAL 128
           SS+ +S  VTGTIFGYR+GK+NFCIQT   STN  LLLELAVPT +LAREMR G LRI L
Sbjct: 77  SSSLSSSTVTGTIFGYRKGKINFCIQTPRKSTNLDLLLELAVPTTVLAREMREGALRIVL 136

Query: 129 ESATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGE 188
           E          S LS P W+MY NG++VGY  +R  S  ++  L  L +V  GAG++ G+
Sbjct: 137 ERNNEKQDDD-SFLSKPFWNMYCNGKRVGYARKRSPSQDDMTALTALSKVVVGAGVVTGK 196

Query: 189 -----DDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
                DD LMYLR +F RV G S +SESFHL DP G+IGQELSIF   S
Sbjct: 197 ELGRFDDELMYLRASFRRVNG-SKESESFHLIDPAGNIGQELSIFIVPS 243

[Close]

BLAST of PI0012300 vs. TAIR 10
Match: AT1G21050.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 177.2 bits (448), Expect = 1.5e-44
Identity = 101/222 (45.50%), Postives = 143/222 (64.41%), Query Frame = 0


Query: 11  AVDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPSS-------AA 70
           +VDC ++ R  +  RSLIE +IP C C + +   Q   +  SS      SS       ++
Sbjct: 26  SVDCSREFRHRRTLRSLIECMIPYC-CTYQQQPYQNDTLSVSSTSSSSSSSDHSSSSQSS 85

Query: 71  NSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREM-RGGVLRIALE--SA 130
           ++V+GT FG+RRG+V+FC+Q  +  ++P+LLLELAVPTA LA+EM   GVLRIALE    
Sbjct: 86  STVSGTFFGHRRGRVSFCLQDAAVGSSPLLLLELAVPTAALAKEMDEEGVLRIALECDRR 145

Query: 131 TAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEG-EDD 190
            ++NS   S+   P WSM+ NGRK+G+ +RR  +  +   LR +  V+ GAG++   E+D
Sbjct: 146 RSSNSRSSSIFDVPVWSMFCNGRKMGFAVRRKVTENDAVFLRMMQSVSVGAGVVPSEEED 205

Query: 191 YLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
            ++YLR  F+RV G+S DSESFH+ +P GS GQELSIF  RS
Sbjct: 206 QMLYLRARFERVTGSS-DSESFHMMNPGGSYGQELSIFLLRS 245

[Close]

BLAST of PI0012300 vs. TAIR 10
Match: AT1G76610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 158.7 bits (400), Expect = 5.5e-39
Identity = 96/211 (45.50%), Postives = 126/211 (59.72%), Query Frame = 0


Query: 12  VDCQKQVRSWQLFRSLIEFLIPTCNCAFTEDQEQQQPMYYSSKPIFPPSSAANSVTGTIF 71
           VDC ++V   +  RSL+E +IP C C +      Q      S            VTGT +
Sbjct: 23  VDCTREVGLRRTLRSLMECVIPYC-CTYQPPPTDQNDTVSVSSSTSSSDQTGVVVTGTFY 82

Query: 72  GYRRGKVNFCIQTNS-SSTNPILLLELAVPTAILAREMRGGVLRIALESATAANSGGRSV 131
           G+RRG V+FC+Q ++  S+ P+LLLELAVPTA LAREM  G LRIAL S +   S   S+
Sbjct: 83  GHRRGHVSFCLQDDTRPSSPPLLLLELAVPTAALAREMEEGFLRIALRSKSNRRS---SI 142

Query: 132 LSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAEGAGIIEGEDDYLMYLRGNFDR 191
            + P WSMY NGRK G+ +RR  +  +V  LR +  V+ GAG+I   +   +YLR  F+R
Sbjct: 143 FNVPVWSMYCNGRKFGFAVRRETTENDVGFLRLMQSVSVGAGVIPNGE--TLYLRAKFER 202

Query: 192 VCGASGDSESFHLRDPNGSIGQELSIFFFRS 222
           V G+S DSESFH+ +  G  GQELSIF  RS
Sbjct: 203 VTGSS-DSESFHMVNQGGGYGQELSIFLSRS 226

[Close]

BLAST of PI0012300 vs. TAIR 10
Match: AT2G37880.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 131.3 bits (329), Expect = 9.3e-31
Identity = 75/182 (41.21%), Postives = 106/182 (58.24%), Query Frame = 0


Query: 50  YYSSKPIFPPSSAANSVTGTIFGYRRGKVNFCIQTNSSSTNPILLLELAVPTAILAREMR 109
           Y  S    P   +   V GTIFG R+G V FC+Q +  S  PILLLEL++ T+ L  EM 
Sbjct: 65  YSISAVTVPQRPSKTMVIGTIFGRRKGHVWFCVQHDRLSVKPILLLELSIATSQLVHEMG 124

Query: 110 GGVLRIALESATAANSGGRSVLSSPAWSMYFNGRKVGYGLRRGASAAEVETLRRLGRVAE 169
            G++R+ALE  T        + S P W+M+ NGRK+G+ +RR A+      L+RL  +  
Sbjct: 125 SGLVRVALECPTRPELKSCLLRSVPVWTMFCNGRKLGFAVRRSANEETRMMLKRLESMTV 184

Query: 170 GAGII----------EGEDDYLMYLRGNFDRVCGASGDSESFHLRDPNGSIGQELSIFFF 222
           GAG++          E + D +MY+R N++ V G+S DSESFHL +P+ +  QELSIF  
Sbjct: 185 GAGVLPSGSGLGGSDESDTDEVMYMRANYEHVVGSS-DSESFHLINPDANSAQELSIFLL 244

The following BLAST results are available for this feature:

BLAST of PI0012300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	6.1e-27	42.37	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of PI0012300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T4C8	4.1e-119	98.20	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25...	[more]
A0A1S3B0Z8	4.1e-119	98.20	protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103484947 PE=4 SV=1	[more]
A0A0A0KG77	2.7e-118	97.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G499200 PE=4 SV=1	[more]
A0A6J1GES2	4.8e-99	83.76	protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453526 PE=4 SV...	[more]
A0A6J1ITF7	3.1e-98	82.28	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478498 PE=4 SV=1	[more]

BLAST of PI0012300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008440568.1	8.5e-119	98.20	PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0036325.1 protein MIZU-KUSSE...	[more]
XP_011657961.1	5.5e-118	97.30	protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN48725.1 hypothetical protein Csa_003...	[more]
XP_038883078.1	8.9e-108	91.63	protein MIZU-KUSSEI 1 [Benincasa hispida]	[more]
XP_022950422.1	9.8e-99	83.76	protein MIZU-KUSSEI 1-like [Cucurbita moschata]	[more]
XP_023544212.1	9.8e-99	83.76	protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo] >KAG6604051.1 Protein MI...	[more]

BLAST of PI0012300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65340.1	1.3e-56	53.91	Protein of unknown function, DUF617	[more]
AT2G22460.1	3.5e-54	53.71	Protein of unknown function, DUF617	[more]
AT1G21050.1	1.5e-44	45.50	Protein of unknown function, DUF617	[more]
AT1G76610.1	5.5e-39	45.50	Protein of unknown function, DUF617	[more]
AT2G37880.1	9.3e-31	41.21	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 66..221 e-value: 9.1E-54 score: 179.7
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 66..220 e-value: 1.2E-53 score: 181.6
None	No IPR available	PANTHER	PTHR31276:SF10	PROTEIN MIZU-KUSSEI 1	coord: 1..222
None	No IPR available	PANTHER	PTHR31276	FAMILY NOT NAMED	coord: 1..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0012300.1	PI0012300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism
molecular_function	GO:0005488	binding