PI0012207 (gene) Melon (PI 482460) v1

Overview
NamePI0012207
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr05: 23049580 .. 23051664 (-)
RNA-Seq ExpressionPI0012207
SyntenyPI0012207
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGATGGAGAGGGAGAGAGAGAGATAGAGAGAGAGTTTGAAGAAGCGGATAGGAAGGGTCGGGTTGTCCAGAGAAAGAGAGAGGGAGAGAGGATCTTTTTATTATTTACCTTCTTGTTGGTTACAACGTTGAATTCTCTCTCTCTCGTTCGCCACGGCTTCCTCTTTAAATTCAATTCGCCGTTTCCTTGTCGGACCGACCGCACAGATTCCCGGAATTCCTCAACATACGCTTCCGTTTTCAAGTAATTCCTTCTTCTTCTTTGTTTTCCTCCTCCCATCCTTGTTGCTATTGCTTGCTTTTCTTTTCTTTCCATTTCTATTTCAATTTCAATTGAATCTCCATCCGCCCGATTTTTATCCTTTTCTTTTGATGGTTGACCCTTTACCGGCGGATCTGGGATCTGTGTACGGTTTCCAAGTTTTTGCGCATTCCACATCTCACATCTGATCGGACGGATTCCCCCCATCACCTGCCTACATATACGATTTGTGACCATCCTCTTACATTTCTACAGAAAACAGAATACCGGAAGGCTGTGTGTGATCATCCCACTACTACAATACAATTCATAGAGAAGACGATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCGATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAACGATATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAATGAGCTCGAATCCGATTTTGGATCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGCATGATGGGAAATCCCCGTGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACAACTGAGCTTGATTCAGTCATTAGAAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATGGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAACCAAAACCAAAACTCTATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCAAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCCGGGGATGAATCCAAGCGTGATGTAGATGGTGGGTCTGCTGCCAGGTTCATGAGCGACCTTAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGTTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGAATATCTTGCTTCTGCCCTGCTTGCTTGCTTATGTATGTGTATGTATGTGTGTTATATTAATTATACTATACAGATGTTTTGCCTCCTGTTCTTAGTAGAACAATTTCTCTTCTTAATATTTTACCAACATTGTATATGCCTTTTCACTCTTACCTCTGCTTTTGTTGCTATTCCTTCACCTCCGATAACAAATCTGTCTTCACCTGTTCCTTAAATTCAACTACAAATATCATCTCACTCTATGTTTTGTGGCAGGGTCAACGATATTGGTTGCGTACTCGCCCATATATATGTTTGTCTGTAGGAATAATAATTTTCATCCCCTGTGATTTCGAAACATATACGGGAATGATATTCGTAGGCTATGTAATTTTAATTATATTTCTACATACACTGCAATTTTTAAAATTCAC

mRNA sequence

GAGATGGAGAGGGAGAGAGAGAGATAGAGAGAGAGTTTGAAGAAGCGGATAGGAAGGGTCGGGTTGTCCAGAGAAAGAGAGAGGGAGAGAGGATCTTTTTATTATTTACCTTCTTGTTGGTTACAACGTTGAATTCTCTCTCTCTCGTTCGCCACGGCTTCCTCTTTAAATTCAATTCGCCGTTTCCTTGTCGGACCGACCGCACAGATTCCCGGAATTCCTCAACATACGCTTCCGTTTTCAAAAAACAGAATACCGGAAGGCTGTGTGTGATCATCCCACTACTACAATACAATTCATAGAGAAGACGATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCGATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAACGATATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAATGAGCTCGAATCCGATTTTGGATCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGCATGATGGGAAATCCCCGTGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACAACTGAGCTTGATTCAGTCATTAGAAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATGGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAACCAAAACCAAAACTCTATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCAAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCCGGGGATGAATCCAAGCGTGATGTAGATGGTGGGTCTGCTGCCAGGTTCATGAGCGACCTTAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGTTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGAATATCTTGCTTCTGCCCTGCTTGCTTGCTTATGTATGTGTATGTATGTGTGTTATATTAATTATACTATACAGATGTTTTGCCTCCTGTTCTTAGTAGAACAATTTCTCTTCTTAATATTTTACCAACATTGTATATGCCTTTTCACTCTTACCTCTGCTTTTGTTGCTATTCCTTCACCTCCGATAACAAATCTGTCTTCACCTGTTCCTTAAATTCAACTACAAATATCATCTCACTCTATGTTTTGTGGCAGGGTCAACGATATTGGTTGCGTACTCGCCCATATATATGTTTGTCTGTAGGAATAATAATTTTCATCCCCTGTGATTTCGAAACATATACGGGAATGATATTCGTAGGCTATGTAATTTTAATTATATTTCTACATACACTGCAATTTTTAAAATTCAC

Coding sequence (CDS)

ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCGATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAACGATATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAATGAGCTCGAATCCGATTTTGGATCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGCATGATGGGAAATCCCCGTGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACAACTGAGCTTGATTCAGTCATTAGAAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATGGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAACCAAAACCAAAACTCTATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCAAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCCGGGGATGAATCCAAGCGTGATGTAGATGGTGGGTCTGCTGCCAGGTTCATGAGCGACCTTAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGTTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Protein sequence

MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNELESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI
Homology
BLAST of PI0012207 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 6.2e-53
Identity = 142/371 (38.27%), Postives = 192/371 (51.75%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           M E   TRYWCH C Q +   +  EIKCP C  GFVEE+         D       N + 
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM--------TDEEIERLTNRQP 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           E  F      S W PI                 E   +  D++ ED +  R      E +
Sbjct: 61  EPGF------SQWNPI-----------------EHPGETMDSDDEDNDLGR------EFE 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGG-- 180
             IRR R ++T+ ++L  I   +A             +ER+  ILIN FNQ + +QG   
Sbjct: 121 GFIRRHRRASTLRRVLDSIHDDLAD-----------DQERDSSILINAFNQALALQGSVL 180

Query: 181 ------ENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVR 240
                  +Q  + N  G L +Y +G GL LLLQH+AE+DP+R GTPPA+KEAV+ALPTV+
Sbjct: 181 DPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVK 240

Query: 241 VELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES 300
           +E      + CSVCLD+ EV  +AK+MPC+HKFH+ CILPWLELHSSCPVCR +LP +E+
Sbjct: 241 IE----EVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEET 300

Query: 301 KRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSF-PWPFNGLF-TSQGNSSAG 360
           K   +  +  R          +G G +    S  S R ++  PW  N LF T +  ++ G
Sbjct: 301 KDLNEPSNIGRVEDSHEEVRADGPGNV----SESSNRPWAIVPW-LNELFSTREAQNAGG 314

Query: 361 SASDSQRDHTS 362
            ++D Q  HTS
Sbjct: 361 VSTDQQSPHTS 314

BLAST of PI0012207 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 82/173 (47.40%), Postives = 108/173 (62.43%), Query Frame = 0

Query: 190 SLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELE--EDSRLQCSVCL 249
           ++GDYF+GPGL+ L+Q +AENDPNRYGTPPA K A++ALP V +          QC+VC+
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 250 DEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES--KRDVDGGSAARFM 309
           D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP D+   +R V G   A+  
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRG---AQGT 288

Query: 310 SDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRD 359
           S  N+G+ +G        + E   R S PWPF       G +   +A   Q D
Sbjct: 289 SGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA--RGPGPAPGDNAETRQED 336

BLAST of PI0012207 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 119/352 (33.81%), Postives = 164/352 (46.59%), Query Frame = 0

Query: 9   YWCHMCSQMVNPIV--DVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNELESDFGS 68
           ++C+ C+Q V   +    +  CP C  GF+EE       D N N + N N N  +S F  
Sbjct: 21  FFCYQCNQTVTISISSSADPFCPICNQGFLEEY-----EDPNPNQSLNFNPNSSDSFFPM 80

Query: 69  ERALSLWAPILLG-MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRR 128
               S   P++ G     P G                  +    +      T L + ++ 
Sbjct: 81  ADPFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQT 140

Query: 129 RRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNQ 188
            RSS T  + +          E+  S  G R                             
Sbjct: 141 LRSSGTHFEFV---------IENHPSDPGNRMP--------------------------- 200

Query: 189 NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV--ELEEDSRLQC 248
              G+ GDYF GPGL+ L+Q +AENDPNRYGTPPA K A+DALPTV+V  ++ +    QC
Sbjct: 201 ---GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQC 260

Query: 249 SVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAAR 308
           +VC+DEFE   + K+MPCKH FH  C+LPWLELH+SCPVCR +LP D+   D +  S   
Sbjct: 261 AVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDP--DYENRSQG- 312

Query: 309 FMSDLNNGNGNGSGEIEGRNSSESGRRFS--FPWPFNGLFTSQGNSSAGSAS 354
                + G+G+G G +EG+ +     RFS   PWPF      Q  S +GS +
Sbjct: 321 -----SQGSGDGQGSVEGQQTP----RFSIQLPWPFR----RQDGSGSGSGA 312

BLAST of PI0012207 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.9e-25
Identity = 83/247 (33.60%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 135 LLQGIRAGMATAESENSYE----------GERSRERERVILINPFNQTIVVQGGENQ--- 194
           L++   A M +  +E SY+          G  S      +LI        + GG+N    
Sbjct: 56  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 115

Query: 195 ---NQNQNSIG-----SLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV 254
              N     IG     + GDYF GPGL+ L++ ++    +R G PPA K ++DALPT+++
Sbjct: 116 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKI 175

Query: 255 ELE--EDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDE 314
             +  + S   C VC DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP   
Sbjct: 176 TQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELP--- 235

Query: 315 SKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGS 359
                  GS++   S  N           GR +S     FS  WPF         SS+ S
Sbjct: 236 -----SRGSSSSTQSSQNRST-------NGRENSRRRNIFSNLWPF--------RSSSSS 278

BLAST of PI0012207 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.2e-25
Identity = 67/166 (40.36%), Postives = 90/166 (54.22%), Query Frame = 0

Query: 189 GSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELE--EDSRLQCSVC 248
           G+ GDYF GPGL+ L + ++     R G PPA + A+DALPT+++       S   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 249 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMS 308
            DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP           S  R   
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG----PSSSQNRTTP 254

Query: 309 DLNNGNGNGSGEIEGR-NSSESGRRFSFPWPFNGLFTSQGNSSAGS 352
             N  + + S     R N +E    FS  WPF     S G+SS+ +
Sbjct: 255 TRNYRSSSSSSSSNSRENGNERRNPFSSFWPFR----SSGSSSSST 291

BLAST of PI0012207 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 6.8e-204
Identity = 359/362 (99.17%), Postives = 360/362 (99.45%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360

Query: 361 SI 363
           SI
Sbjct: 361 SI 362

BLAST of PI0012207 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.4e-201
Identity = 357/366 (97.54%), Postives = 358/366 (97.81%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 363
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of PI0012207 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.4e-201
Identity = 357/366 (97.54%), Postives = 358/366 (97.81%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 363
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of PI0012207 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.8e-172
Identity = 320/374 (85.56%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GN +SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGNRVSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERS--------RERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAGMAT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSS 360
            DESKRD DGGS ARFM    +G+G+GSGEI+GRN+S +GRRFSFPWPFNGLF+SQGNSS
Sbjct: 301 ADESKRDADGGS-ARFMGS-GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSS 360

Query: 361 AGSASDSQRDHTSI 363
            GS S+SQRDHTSI
Sbjct: 361 GGSGSESQRDHTSI 369

BLAST of PI0012207 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.3e-169
Identity = 317/380 (83.42%), Postives = 336/380 (88.42%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEE+GN +SSDNN  NNNNN+NN L
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDD+N   EH DGEGNR SSE  
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERS----------RERERVILI 180
           ELDSVI RRRRSSATILQLLQGIRAGMAT ESENSYEGERS          RERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVD 240
           NPFNQTIVVQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVD
Sbjct: 181 NPFNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 300
           ALPTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQ
Sbjct: 241 ALPTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQ 300

Query: 301 LPGDESKRDVDGGSAARFMSDLNNGN----GNGSGEIEGRNSSESGRRFSFPWPFNGLFT 360
           LP DESKRD DGGS ARFM +L+NGN    G+GSGEI+GR +S  GRRFSFPWPFNGLF+
Sbjct: 301 LPADESKRDTDGGS-ARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFS 360

Query: 361 SQGNSSAGSASDSQRDHTSI 363
           SQGNSS GS S+SQRDHT I
Sbjct: 361 SQGNSSGGSESESQRDHTPI 375

BLAST of PI0012207 vs. NCBI nr
Match: XP_031739817.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus])

HSP 1 Score: 712.2 bits (1837), Expect = 2.2e-201
Identity = 358/362 (98.90%), Postives = 359/362 (99.17%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYE ERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYE-ERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360

Query: 361 SI 363
           SI
Sbjct: 361 SI 361

BLAST of PI0012207 vs. NCBI nr
Match: XP_008449904.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0040122.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK21744.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 357/366 (97.54%), Postives = 358/366 (97.81%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 363
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of PI0012207 vs. NCBI nr
Match: XP_038901761.1 (E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida])

HSP 1 Score: 645.6 bits (1664), Expect = 2.6e-181
Identity = 330/363 (90.91%), Postives = 342/363 (94.21%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN IS   NDNN+NN+NNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGIS---NDNNDNNSNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDNEHEDGEGNRVSSE T+LD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTDLD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGE 
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGE- 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
            NQNQN IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDALPTVRVE   DS 
Sbjct: 181 -NQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVE-PVDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQC+VCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD DGGS
Sbjct: 241 LQCAVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDADGGS 300

Query: 301 AARFMSDLNNGNGNGSGE-IEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDH 360
             RFM DLNN NG+GSGE I+GRN+S +GRRFSFPWPFNGLF++QGNSS GSASDSQRDH
Sbjct: 301 -TRFMGDLNNANGSGSGEIIDGRNASGTGRRFSFPWPFNGLFSAQGNSSGGSASDSQRDH 356

Query: 361 TSI 363
            SI
Sbjct: 361 ASI 356

BLAST of PI0012207 vs. NCBI nr
Match: KAE8649424.1 (hypothetical protein Csa_021650 [Cucumis sativus])

HSP 1 Score: 632.9 bits (1631), Expect = 1.7e-177
Identity = 323/362 (89.23%), Postives = 323/362 (89.23%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGN ISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 325

Query: 361 SI 363
           SI
Sbjct: 361 SI 325

BLAST of PI0012207 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 615.1 bits (1585), Expect = 3.7e-172
Identity = 320/374 (85.56%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GN +SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNRVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGNRVSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERS--------RERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAGMAT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QN N+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSS 360
            DESKRD DGGS ARFM    +G+G+GSGEI+GRN+S +GRRFSFPWPFNGLF+SQGNSS
Sbjct: 301 ADESKRDADGGS-ARFMGS-GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSS 360

Query: 361 AGSASDSQRDHTSI 363
            GS S+SQRDHTSI
Sbjct: 361 GGSGSESQRDHTSI 369

BLAST of PI0012207 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.0e-103
Identity = 209/391 (53.45%), Postives = 263/391 (67.26%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           M+E +A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EE+     +          +  + 
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMS---GNGGGGGGRGIRDVQDS 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN-----EHEDGEGNRVSSE 120
           E+DFG++RALSLWAPILLGMM +PR RRRFR  EF +++DDN      + DG GN  ++ 
Sbjct: 61  ETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNS 120

Query: 121 TT-------------------ELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGE 180
                                E +S++ RRRRSS  ILQLLQGIRAG+A+    +    +
Sbjct: 121 NNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNWD 180

Query: 181 RSRERERVILINPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRY 240
            SRER+RVI+INP+NQ++VV    +QNQN  S+ SLGDYF+GPGLDLLLQH+AENDPNR 
Sbjct: 181 NSRERDRVIMINPYNQSLVVP--SDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQ 240

Query: 241 GTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLE 300
           GTPPA+KEAV+ALPTV++       LQCSVCLD+FE   EAKEMPCKHKFH  CI+PWLE
Sbjct: 241 GTPPARKEAVEALPTVKIM----EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLE 300

Query: 301 LHSSCPVCRHQLPG-----DESKRDVDGGSAARFMSDLNNGN-----GNGSGEIEGRNSS 357
           LHSSCPVCR +LP      DE+K D +     R + + +NGN     GN     E    S
Sbjct: 301 LHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRS 360

BLAST of PI0012207 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.7e-88
Identity = 191/380 (50.26%), Postives = 248/380 (65.26%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+  +I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN             
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ------RVSSETTELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERV 184
                 R      E +S++ RRRRSSATILQLLQGIRAG+A +E E+S         +R 
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRD 180

Query: 185 ILINPFNQTIVVQGGE--NQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQ 244
           +L    NQ+ VVQG    NQN+N  S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+
Sbjct: 181 LL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPAR 240

Query: 245 KEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCP 304
           KE VD LPTV++       LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 KEVVDNLPTVKI----SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCP 300

Query: 305 VCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLF 362
           VCR++LP D+  +        R + ++N  N N   E + RNS+ S RRFSFPWPF+GLF
Sbjct: 301 VCRYELPPDDETKVNPVRPRTRTL-EINVSNEN--VEDDARNSNVSERRFSFPWPFSGLF 356

BLAST of PI0012207 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.7e-88
Identity = 191/380 (50.26%), Postives = 248/380 (65.26%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+  +I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN             
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ------RVSSETTELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERV 184
                 R      E +S++ RRRRSSATILQLLQGIRAG+A +E E+S         +R 
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRD 180

Query: 185 ILINPFNQTIVVQGGE--NQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQ 244
           +L    NQ+ VVQG    NQN+N  S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+
Sbjct: 181 LL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPAR 240

Query: 245 KEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCP 304
           KE VD LPTV++       LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 KEVVDNLPTVKI----SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCP 300

Query: 305 VCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLF 362
           VCR++LP D+  +        R + ++N  N N   E + RNS+ S RRFSFPWPF+GLF
Sbjct: 301 VCRYELPPDDETKVNPVRPRTRTL-EINVSNEN--VEDDARNSNVSERRFSFPWPFSGLF 356

BLAST of PI0012207 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 2.4e-84
Identity = 182/375 (48.53%), Postives = 239/375 (63.73%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNEL 60
           M+E + TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEE+ +D   D++D  +   NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDD-----DNEHEDGEGNRVSSE 120
                     SLWAPIL+ +M +P  RRR + +E  +D+      +N  +DGE N +  +
Sbjct: 61  ----------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-NDLDWQ 120

Query: 121 TTELDSVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVV 180
             E+  + RRRR SA +LQLLQGIRAG+ + ESE++  G       RVILIN  NQTI V
Sbjct: 121 LQEI--LRRRRRHSAAVLQLLQGIRAGL-SVESEST--GNGGNNPGRVILINTSNQTITV 180

Query: 181 QGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVEL 240
           Q   + + +    GSLGDYF+GPG ++LLQ +AENDPNRYGTPPA+KEAV+AL TV++E 
Sbjct: 181 Q--NSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIE- 240

Query: 241 EEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD 300
                LQCSVCLD+FE+  EAK MPC HKFH+ C+LPWLELHSSCPVCR+QLP DE+K D
Sbjct: 241 ---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTD 300

Query: 301 -----VDGGSAARFMSDLNNGNGNGSG------------EIEGRNSSESGRRFSFPWPFN 354
                 D   ++   +  ++G  N  G            E E  N +  G  FS PWPF+
Sbjct: 301 SVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFS 350

BLAST of PI0012207 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.5e-65
Identity = 158/340 (46.47%), Postives = 204/340 (60.00%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEIGNDISSDNNDNNNNNNNNNE 60
           M++   T YWCHMCS+ V P++  E IKC FC+SGFVEE+      DNND++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEF-----DDDDDDNEHEDGEGNRVS- 120
                  + A SLW PIL+ MM N   +      +      D+D+D+++ +DG+ N    
Sbjct: 61  -------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE 120

Query: 121 -SETTELDSVIR-RRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQ 180
              T +L+ + R R R S  I+ LLQGIRAG+   ESEN+ +   +   E V+LIN FNQ
Sbjct: 121 IDITHQLEEIRRIRTRHSTAIVNLLQGIRAGL-LIESENNEDNPDN--SELVVLINSFNQ 180

Query: 181 TIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAEND-PNRYGTPPAQKEAVDALPT 240
            I V        +  S GSLGDYF+GPG + LLQ +AEND  NRYGTPPA KEAV+AL  
Sbjct: 181 RIRVHQDSVDTTSVPS-GSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAM 240

Query: 241 VRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP-- 300
           V++   EDS LQCSVCLD+FE+  EAKEMPCKHKFH+ C+LPWLELHSSCPVCR+ LP  
Sbjct: 241 VKI---EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTG 300

Query: 301 -GDESKRDVDGGSAARFMSDLNNGN--GNGSGEIEGRNSS 326
             DE K D +         D++N +   NGS      N+S
Sbjct: 301 DDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AVN26.2e-5338.27E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
P0CH301.3e-3747.40E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN71.3e-3733.81E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q94AK41.9e-2533.60E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221974.2e-2540.36Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Match NameE-valueIdentityDescription
A0A0A0KWR36.8e-20499.17RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE... [more]
A0A1S3BNR75.4e-20197.54RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ... [more]
A0A5D3DDU35.4e-20197.54RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1FWF91.8e-17285.56RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781... [more]
A0A6J1JDY59.3e-16983.42RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P... [more]
Match NameE-valueIdentityDescription
XP_031739817.12.2e-20198.90LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus][more]
XP_008449904.11.1e-20097.54PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1... [more]
XP_038901761.12.6e-18190.91E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquit... [more]
KAE8649424.11.7e-17789.23hypothetical protein Csa_021650 [Cucumis sativus][more]
XP_022944289.13.7e-17285.56E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G56340.13.0e-10353.45RING/U-box superfamily protein [more]
AT4G26400.24.7e-8850.26RING/U-box superfamily protein [more]
AT4G26400.14.7e-8850.26RING/U-box superfamily protein [more]
AT1G55530.12.4e-8448.53RING/U-box superfamily protein [more]
AT3G13430.11.5e-6546.47RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 243..283
e-value: 7.0E-8
score: 42.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 242..284
e-value: 4.0E-13
score: 49.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 243..284
score: 12.386007
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 206..290
e-value: 5.0E-22
score: 79.4
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 7..39
e-value: 4.0E-12
score: 46.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..362
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 289..362
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..339
NoneNo IPR availablePANTHERPTHR15710:SF22RING/U-BOX SUPERFAMILY PROTEINcoord: 3..339
NoneNo IPR availableCDDcd16669RING-H2_RNF181coord: 242..287
e-value: 4.44158E-23
score: 88.5817
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 232..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012207.1PI0012207.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity