PI0012030 (gene) Melon (PI 482460) v1

BLAST of PI0012030 vs. ExPASy Swiss-Prot
Match: Q41059 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (Fragment) OS=Pisum sativum OX=3888 GN=SBEII PE=1 SV=1)

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 598/769 (77.76%), Postives = 662/769 (86.09%), Query Frame = 0

Query: 61  STYKRVHSPAIS-SVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLL 120
           S Y++V S     SVMTDD ST+ S +E  ENIGIL+ D  L+PFKDHFKYR+ RY    
Sbjct: 44  SIYQKVSSGFKGVSVMTDDKSTMPSVEEDFENIGILNVDSSLEPFKDHFKYRLKRYLHQK 103

Query: 121 NLLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKN 180
            L++++EGGL EFA+GYLKFGFNREEDGI YREWAPAAQEAQI+GDFNGW+G+N  MEK+
Sbjct: 104 KLIEEYEGGLQEFAKGYLKFGFNREEDGISYREWAPAAQEAQIIGDFNGWNGSNLHMEKD 163

Query: 181 EFGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGV 240
           +FG+WSI+I D  G PAI HNSRVKFRFKH +GVW+DRIPAWIKYATVDPT+FAAPYDGV
Sbjct: 164 QFGVWSIQIPDADGNPAIPHNSRVKFRFKHSDGVWVDRIPAWIKYATVDPTRFAAPYDGV 223

Query: 241 YWDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNT 300
           YWDPP SERY+FKHPRP KP APR+YEAHVGMSSSEPR++SYREFAD VLPRI+ENNYNT
Sbjct: 224 YWDPPLSERYQFKHPRPPKPKAPRIYEAHVGMSSSEPRINSYREFADDVLPRIRENNYNT 283

Query: 301 VQLMAIMEHSYYASFGYHVTN-FFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHAS 360
           VQLMA+MEHSYYASF YHVT  FFAVSSRSG+PEDLKYLIDKAH LGL VLMD++HSHAS
Sbjct: 284 VQLMAVMEHSYYASFWYHVTKPFFAVSSRSGSPEDLKYLIDKAHSLGLNVLMDVIHSHAS 343

Query: 361 NNVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQ 420
           NNVTDGLNGFDVGQS+Q SYFH GDRGYHKLWDSRLFNYANW+   FLLSN+RWWLEEY+
Sbjct: 344 NNVTDGLNGFDVGQSSQQSYFHAGDRGYHKLWDSRLFNYANWK-SSFLLSNLRWWLEEYK 403

Query: 421 FDGFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVI 480
           FDGFRFDGVTSMLYHHHGINM FTG+YNEYFSE TDVDAVVYLMLAN+L H ILPDAT I
Sbjct: 404 FDGFRFDGVTSMLYHHHGINMAFTGDYNEYFSEETDVDAVVYLMLANSLVHDILPDATDI 463

Query: 481 AEDVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYS 540
           AEDVSGMPGLGRPV E GIGFDYRL MAIPDKWIDYLKNK D EWSM EIS NLTNRRY+
Sbjct: 464 AEDVSGMPGLGRPVSEVGIGFDYRLAMAIPDKWIDYLKNKKDSEWSMKEISLNLTNRRYT 523

Query: 541 EKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMAL 600
           EKC+SYAESHDQSIVGDKTIAFLLMD+EMYS MS LT  SP +ERGI+LHKMIHFIT+AL
Sbjct: 524 EKCVSYAESHDQSIVGDKTIAFLLMDEEMYSSMSCLTMLSPTIERGISLHKMIHFITLAL 583

Query: 601 GGEGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCR-RQWNLPDTNHLRYKFLNAFDRAMNA 660
           GGEGYLNFMGNEFGHPEWIDFPR+GNGWSY+KCR  QWNL DTNHLRYKF+NAFDRAMN 
Sbjct: 584 GGEGYLNFMGNEFGHPEWIDFPREGNGWSYEKCRLTQWNLVDTNHLRYKFMNAFDRAMNL 643

Query: 661 LDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVA 720
           LD+KFS LAS+KQIVS T  EDKVIVFERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVA
Sbjct: 644 LDDKFSILASTKQIVSSTNNEDKVIVFERGDLVFVFNFHPENTYEGYKVGCDLPGKYRVA 703

Query: 721 LDSDALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDES 780
           LDSDA +FGGHGRVGH  D FTSPEGIPG+PETNFNNRPNSFKVLSP  TCVVYY+VDE 
Sbjct: 704 LDSDATEFGGHGRVGHDADQFTSPEGIPGIPETNFNNRPNSFKVLSPPHTCVVYYRVDER 763

Query: 781 KEKEKDDLVGGANEDVFVRHSEDSEELAGCEEENDIAVGEISKTEDGDD 827
           +E+  +  +G   E+ F     D   +     E++ +   + + ED  +
Sbjct: 764 QEESNNPNLGSV-EETFAAADTDVARIPDVSMESEDS--NLDRIEDNSE 808

BLAST of PI0012030 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 567/691 (82.05%), Postives = 628/691 (90.88%), Query Frame = 0

Query: 67  HSPAISSVMTDDTSTVSSTDESM--ENIGILSHDPGLKPFKDHFKYRIGRYADLLNLLDK 126
           HS AIS+V+TDD ST++  +E +  ENIG+L+ DP L+P+ DHF++R+ RY D   L++K
Sbjct: 69  HSSAISAVLTDDNSTMAPLEEDVKTENIGLLNLDPTLEPYLDHFRHRMKRYVDQKMLIEK 128

Query: 127 HEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIW 186
           +EG L+EFA+GYLKFGFNRE+  IVYREWAPAAQE +++GDFNGW+G+NH MEK++FG+W
Sbjct: 129 YEGPLEEFAQGYLKFGFNREDGCIVYREWAPAAQEDEVIGDFNGWNGSNHMMEKDQFGVW 188

Query: 187 SIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPP 246
           SI+I D+  KP I HNSRVKFRFKHGNGVW+DRIPAWIKYAT D TKFAAPYDGVYWDPP
Sbjct: 189 SIRIPDVDSKPVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPP 248

Query: 247 SSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMA 306
            SERY FK+PRP KP APR+YEAHVGMSSSEPRV+SYREFAD VLPRIK NNYNTVQLMA
Sbjct: 249 PSERYHFKYPRPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMA 308

Query: 307 IMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDG 366
           IMEHSYY SFGYHVTNFFAVSSR G PEDLKYLIDKAH LGL+VL+D+VHSHASNNVTDG
Sbjct: 309 IMEHSYYGSFGYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDG 368

Query: 367 LNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRF 426
           LNGFD+GQ +Q+SYFH G+RGYHKLWDSRLFNYANWEVLRFLLSN+RWWLEEY FDGFRF
Sbjct: 369 LNGFDIGQGSQESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRF 428

Query: 427 DGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSG 486
           DG+TSMLY HHGINMGFTGNYNEYFSEATDVDAVVYLMLANNL H I PDATVIAEDVSG
Sbjct: 429 DGITSMLYVHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSG 488

Query: 487 MPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISY 546
           MPGLGRPV EGGIGFDYRL MAIPDKWIDYLKNK DE+WSM E++ +LTNRRY+EKCI+Y
Sbjct: 489 MPGLGRPVSEGGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSLTNRRYTEKCIAY 548

Query: 547 AESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGGEGYL 606
           AESHDQSIVGDKTIAFLLMDKEMYSGMS LT+ASPVV+RGIALHKMIHF TMALGGEGYL
Sbjct: 549 AESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYL 608

Query: 607 NFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSF 666
           NFMGNEFGHPEWIDFPR+GN WSYDKCRRQWNL D+ HLRYKF+NAFDRAMN+LDEKFSF
Sbjct: 609 NFMGNEFGHPEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKFSF 668

Query: 667 LASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALD 726
           LAS KQIVS   +++KV+VFERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA +
Sbjct: 669 LASGKQIVSSMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDAWE 728

Query: 727 FGGHGRVGHGIDHFTSPEGIPGVPETNFNNR 756
           FGGHGR GH +DHFTSPEGIPGVPETNFN R
Sbjct: 729 FGGHGRTGHDVDHFTSPEGIPGVPETNFNGR 759

BLAST of PI0012030 vs. ExPASy Swiss-Prot
Match: Q01401 (1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SBE1 PE=1 SV=2)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 546/700 (78.00%), Postives = 619/700 (88.43%), Query Frame = 0

Query: 81  TVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLNLLDKHEGGLDEFARGYLKFG 140
           T+ +  E ++++ I   DP L+ FKDHF YRI RY D   L++KHEGGL+EF++GYLKFG
Sbjct: 65  TMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQKCLIEKHEGGLEEFSKGYLKFG 124

Query: 141 FNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWSIKIYDLGGKPAISHN 200
            N  +   +YREWAPAAQEAQ++G+FN W+G  H MEK++FGIWSIKI  + GKPAI HN
Sbjct: 125 INTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKMEKDKFGIWSIKISHVNGKPAIPHN 184

Query: 201 SRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHPRPAKPN 260
           S+VKFRF+HG G W+DRIPAWI+YAT D +KF APYDGV+WDPP+ ERY FKHPRP KP+
Sbjct: 185 SKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGAPYDGVHWDPPACERYVFKHPRPPKPD 244

Query: 261 APRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTN 320
           APR+YEAHVGMS  EP VS+YREFAD VLPRI+ NNYNTVQLMAIMEHSYYASFGYHVTN
Sbjct: 245 APRIYEAHVGMSGEEPEVSTYREFADNVLPRIRANNYNTVQLMAIMEHSYYASFGYHVTN 304

Query: 321 FFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQSTQDSYFH 380
           FFAVSSRSGTPEDLKYL+DKAH LGLRVLMD+VHSHASNNVTDGLNG+DVGQ+T +SYFH
Sbjct: 305 FFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFH 364

Query: 381 TGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMG 440
           TGDRGYHKLWDSRLFNYANWEVLRFLLSN+R+W++E+ FDGFRFDGVTSMLYHHHGIN G
Sbjct: 365 TGDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKG 424

Query: 441 FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFEGGIGFD 500
           FTGNY EYFS  TDVDA+VY+MLAN+L H +LP+AT++AEDVSGMP L RPV EGG+GFD
Sbjct: 425 FTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVLCRPVDEGGVGFD 484

Query: 501 YRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQSIVGDKTIAF 560
           +RL MAIPD+WIDYLKNK D +WSM EI   LTNRRY+EKCI+YAESHDQSIVGDKTIAF
Sbjct: 485 FRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAF 544

Query: 561 LLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFP 620
           LLMDKEMY+GMS L  ASP + RGIAL KMIHFITMALGG+GYLNFMGNEFGHPEWIDFP
Sbjct: 545 LLMDKEMYTGMSDLQPASPTINRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFP 604

Query: 621 RDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDK 680
           R+GN WSYDKCRRQW+L DT+HLRYK++NAFD+AMNAL+E+FSFL+SSKQIVS   E+DK
Sbjct: 605 REGNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDK 664

Query: 681 VIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHGIDHFTS 740
           VIVFERGDLVFVFNFHP  TY GYKVGCDLPGKYRVALDSDAL FGGHGRVGH +DHFTS
Sbjct: 665 VIVFERGDLVFVFNFHPNKTYKGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTS 724

Query: 741 PEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 781
           PEG+PGVPETNFNNRPNSFKVLSP RTCV YY+VDE +E+
Sbjct: 725 PEGMPGVPETNFNNRPNSFKVLSPPRTCVAYYRVDEDREE 764

BLAST of PI0012030 vs. ExPASy Swiss-Prot
Match: O23647 (1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE2.1 PE=1 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 1.2e-245
Identity = 416/710 (58.59%), Postives = 510/710 (71.83%), Query Frame = 0

Query: 98  DPGLKPFKDHFKYRIGRYADLLNLLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 157
           DP L   ++H  YR G+Y  L   +DK+EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIVGDFNGWDGTNHCMEKNEFGIWSIKI-YDLGGKPAISHNSRVKFRFKHGNGVWID 217
           + A ++GDFN W+  +  M +N+FG+W I +  +  G PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEP 277
            IPAWIKY+   P +   PY+GVY+DPP  ++Y FKHPRP KP + R+YE+HVGMSS+EP
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
           ++++Y  F D VLPRIK+  YN VQ+MAI EH+YYASFGYHVTNFFA SSR GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFN 397
           LIDKAH LGL VLMDIVHSHAS N  DGL+ FD    T   YFH+G RGYH +WDSRLFN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGNYNEYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+L H + P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTN 577
            K DE+W +G+I++ LTNRR+ EKC+ YAESHDQ++VGDKTIAF LMDK+MY  M+    
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPVVERGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPRD-----------GNG 637
           A+P V+RGIALHKMI  ITM LGGEGYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFE 697
            SYDKCRR+++L D  +LRY  L  FDRAM  L+E + F+ S  Q +S   E D+VIVFE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHGIDHFTSPEGIP 757
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDLV--GGANEDV 794
              +   ++RP SF V +P RT VVY  VD+  + E+  LV  G   EDV
Sbjct: 824 ---DGRHDDRPCSFMVYAPCRTAVVYAAVDDDDDDERSSLVPIGLLPEDV 858

BLAST of PI0012030 vs. ExPASy Swiss-Prot
Match: Q08047 (1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays OX=4577 GN=SBE1 PE=1 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.5e-243
Identity = 410/694 (59.08%), Postives = 500/694 (72.05%), Query Frame = 0

Query: 94  ILSHDPGLKPFKDHFKYRIGRYADLLNLLDKHEGGLDEFARGYLKFGFNREEDGIVYREW 153
           I   DP L+ +K H +YR   Y  + + +D+HEGGL+ F+R Y KFGFN   +GI YREW
Sbjct: 121 IFQIDPMLQGYKYHLEYRYSLYRRIRSDIDEHEGGLEAFSRSYEKFGFNASAEGITYREW 180

Query: 154 APAAQEAQIVGDFNGWDGTNHCMEKNEFGIWSIKI-YDLGGKPAISHNSRVKFRFKHGNG 213
           AP A  A +VGD N WD     M KNEFG+W I +  +  G   I H SRVK R    +G
Sbjct: 181 APGAFSAALVGDVNNWDPNADRMSKNEFGVWEIFLPNNADGTSPIPHGSRVKVRMDTPSG 240

Query: 214 VWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHPRPAKPNAPRVYEAHVGMS 273
           +  D IPAWIKY+   P +   PYDG+Y+DPP   +Y F+H +P +P + R+YE HVGMS
Sbjct: 241 I-KDSIPAWIKYSVQAPGEI--PYDGIYYDPPEEVKYVFRHAQPKRPKSLRIYETHVGMS 300

Query: 274 SSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPE 333
           S EP++++Y  F D VLPRIK+  YN VQ+MAI EHSYY SFGYHVTNFFA SSR GTPE
Sbjct: 301 SPEPKINTYVNFRDEVLPRIKKLGYNAVQIMAIQEHSYYGSFGYHVTNFFAPSSRFGTPE 360

Query: 334 DLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDS 393
           DLK LID+AH LGL VLMD+VHSHAS+N  DGLNGFD    T   YFH+G RG+H +WDS
Sbjct: 361 DLKSLIDRAHELGLLVLMDVVHSHASSNTLDGLNGFD---GTDTHYFHSGPRGHHWMWDS 420

Query: 394 RLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMGFTGNYNEYFSEA 453
           RLFNY NWEVLRFLLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGN+NEYF  A
Sbjct: 421 RLFNYGNWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVTFTGNFNEYFGFA 480

Query: 454 TDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWI 513
           TDVDAVVYLML N+L H + P+A  I EDVSGMP    PV +GG+GFDYR+ MA+ DKWI
Sbjct: 481 TDVDAVVYLMLVNDLIHGLYPEAVTIGEDVSGMPTFALPVHDGGVGFDYRMHMAVADKWI 540

Query: 514 DYLKNKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMS 573
           D LK ++DE W MG+I   LTNRR+ EKC++YAESHDQ++VGDKTIAF LMDK+MY  M+
Sbjct: 541 DLLK-QSDETWKMGDIVHTLTNRRWLEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMA 600

Query: 574 SLTNASPVVERGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPR----------- 633
               ++P ++RGIALHKMI  ITM LGGEGYLNFMGNEFGHPEWIDFPR           
Sbjct: 601 LDRPSTPTIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGPQRLPSGKFI 660

Query: 634 DGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKV 693
            GN  SYDKCRR+++L D ++LRY  +  FD+AM  L++K+ F+ S  Q +S   EEDKV
Sbjct: 661 PGNNNSYDKCRRRFDLGDADYLRYHGMQEFDQAMQHLEQKYEFMTSDHQYISRKHEEDKV 720

Query: 694 IVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHGIDHFTSP 753
           IVFE+GDLVFVFNFH  N+Y  Y++GC  PG Y+V LDSDA  FGG  R+ H  +HFT+ 
Sbjct: 721 IVFEKGDLVFVFNFHCNNSYFDYRIGCRKPGVYKVVLDSDAGLFGGFSRIHHAAEHFTA- 780

Query: 754 EGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVD 776
                  + + +NRP SF V +P+RTCVVY  V+
Sbjct: 781 -------DCSHDNRPYSFSVYTPSRTCVVYAPVE 799

BLAST of PI0012030 vs. ExPASy TrEMBL
Match: A0A0A0KGQ9 (1,4-alpha-glucan branching enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G357030 PE=3 SV=1)

HSP 1 Score: 1681.0 bits (4352), Expect = 0.0e+00
Identity = 805/849 (94.82%), Postives = 823/849 (96.94%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           MLLS CVLPSTA+ASLSLSAKK  ISDSRN+WPSLRQQTELNFWSRMSHHRQMNPF+FPY
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKRVHSPAISSVMT+DTSTVSSTDESMENIGILSHDPGLKPFKDHFKYR+GRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIK+YDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINMGF+GNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNLPDT+HLRYKFLNAFD AMNALDE
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGGHGRVGH IDHFTSPEGIPGVPETNFNNRPNSFK+LSPARTCVVYYKVDESKEK
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESKEK 780

Query: 781 EKDDLVGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTED---------GDDIDSN 840
           EKDDLVG ANEDVF RH  EDSE LAGC+EENDIAVGEISKTED          DD+DSN
Sbjct: 781 EKDDLVGSANEDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDTSKPEDDDVDSN 840

BLAST of PI0012030 vs. ExPASy TrEMBL
Match: E5GBZ3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1672.9 bits (4331), Expect = 0.0e+00
Identity = 807/856 (94.28%), Postives = 823/856 (96.14%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           MLLSPCVLPSTAIASLSLSAKK FISDSRN+WPSLRQQTELNFWSRMSHHRQMNPFIFPY
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHDPGLKPFKDHFKYR+GRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGW+GTNHCMEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKPN PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+M FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNL DT+HLRYKFLNAFDRAMNALDE
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 EKDDLVGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTEDG---------DDIDSN 840
           EKDDLV   +EDV  RH  EDSE LAG EEENDIAVG+ISKTED          DD+DSN
Sbjct: 781 EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDDVDSN 840

Query: 841 EIEDLPVRGTRARIID 847
           +IEDLPVRG RA+I D
Sbjct: 841 KIEDLPVRGNRAKISD 856

BLAST of PI0012030 vs. ExPASy TrEMBL
Match: A0A1S3CMX9 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103502624 PE=3 SV=1)

HSP 1 Score: 1672.9 bits (4331), Expect = 0.0e+00
Identity = 807/856 (94.28%), Postives = 823/856 (96.14%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           MLLSPCVLPSTAIASLSLSAKK FISDSRN+WPSLRQQTELNFWSRMSHHRQMNPFIFPY
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHDPGLKPFKDHFKYR+GRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGW+GTNHCMEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKPN PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+M FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNL DT+HLRYKFLNAFDRAMNALDE
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 EKDDLVGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTEDG---------DDIDSN 840
           EKDDLV   +EDV  RH  EDSE LAG EEENDIAVG+ISKTED          DD+DSN
Sbjct: 781 EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDDVDSN 840

Query: 841 EIEDLPVRGTRARIID 847
           +IEDLPVRG RA+I D
Sbjct: 841 KIEDLPVRGNRAKISD 856

BLAST of PI0012030 vs. ExPASy TrEMBL
Match: A0A5A7T693 (1,4-alpha-glucan branching enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00200 PE=3 SV=1)

HSP 1 Score: 1552.7 bits (4019), Expect = 0.0e+00
Identity = 746/791 (94.31%), Postives = 760/791 (96.08%), Query Frame = 0

Query: 66  VHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLNLLDKH 125
           VHSPAISSVMTDDTSTVSS DESMENIGILSHDPGLKPFKDHFKYR+GRY DLLNLLDKH
Sbjct: 24  VHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLNLLDKH 83

Query: 126 EGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS 185
           EGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS
Sbjct: 84  EGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS 143

Query: 186 IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPS 245
           IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPP 
Sbjct: 144 IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPP 203

Query: 246 SERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI 305
            ERYEFKHPRPAKPN PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI
Sbjct: 204 LERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI 263

Query: 306 MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGL 365
           MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNNVTDGL
Sbjct: 264 MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGL 323

Query: 366 NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD 425
           NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD
Sbjct: 324 NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD 383

Query: 426 GVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM 485
           GVTSMLYHHHGI+M FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM
Sbjct: 384 GVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM 443

Query: 486 PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISYA 545
           PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEKCISYA
Sbjct: 444 PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEKCISYA 503

Query: 546 ESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGGEGYLN 605
           ESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGGEGYLN
Sbjct: 504 ESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGGEGYLN 563

Query: 606 FMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFL 665
           FMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNL DT+HLRYKFLNAFDRAMNALDEKFSFL
Sbjct: 564 FMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDEKFSFL 623

Query: 666 ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDF 725
           ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDSDA DF
Sbjct: 624 ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAGDF 683

Query: 726 GGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL 785
           GG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL
Sbjct: 684 GGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL 743

Query: 786 VGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTEDG---------DDIDSNEIEDL 845
           VG  +EDV  RH  EDSE LAG EEENDIAVG+ISKTED          DD+DSN+IEDL
Sbjct: 744 VGSVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDDVDSNKIEDL 803

Query: 846 PVRGTRARIID 847
           PVRG RA+I D
Sbjct: 804 PVRGNRAKISD 814

BLAST of PI0012030 vs. ExPASy TrEMBL
Match: A0A6J1DI71 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111020717 PE=3 SV=1)

HSP 1 Score: 1534.6 bits (3972), Expect = 0.0e+00
Identity = 740/858 (86.25%), Postives = 785/858 (91.49%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           ML S CVLP T++ASLSLSAKK F + SRN+WPSLR+Q EL F SRMSHHR MNPF+ P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKR HS AIS VMTD+  T++S DE MEN+GILSHDPGL+PFKDHFKYRIGRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LL+KHEGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGWDG+NH MEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIK+ D GGKPAI+HNSRVKFRFKHGNGVWIDRIPAWI+YATVDPT+FAAPYDGVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEF+HPRPAKP APR+YEAHVGMSSSEPRV+SYREFADF+LPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD++HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+Q SYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNL HSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKT+AFLLMDKEMYSGMS LT ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNLPDT+HLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+S T EEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGGHGR+GH  DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYY+VDESKEK
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESKEK 780

Query: 781 EKDDLVG---GANEDVFVRHSEDSEELAGCEEENDIAVGE------------ISKTEDGD 840
           E  D VG       DV  RH+E+ +E     EE DIAVGE             SK+EDGD
Sbjct: 781 ENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVEDDNIDTSKSEDGD 840

Query: 841 DIDSNEIEDLPVRGTRAR 844
           D D ++ EDLPV+ TRAR
Sbjct: 841 D-DPSKSEDLPVKETRAR 857

BLAST of PI0012030 vs. NCBI nr
Match: XP_004144236.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic [Cucumis sativus] >KGN47537.1 hypothetical protein Csa_019021 [Cucumis sativus])

HSP 1 Score: 1681.0 bits (4352), Expect = 0.0e+00
Identity = 805/849 (94.82%), Postives = 823/849 (96.94%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           MLLS CVLPSTA+ASLSLSAKK  ISDSRN+WPSLRQQTELNFWSRMSHHRQMNPF+FPY
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKRVHSPAISSVMT+DTSTVSSTDESMENIGILSHDPGLKPFKDHFKYR+GRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIK+YDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINMGF+GNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNLPDT+HLRYKFLNAFD AMNALDE
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGGHGRVGH IDHFTSPEGIPGVPETNFNNRPNSFK+LSPARTCVVYYKVDESKEK
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESKEK 780

Query: 781 EKDDLVGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTED---------GDDIDSN 840
           EKDDLVG ANEDVF RH  EDSE LAGC+EENDIAVGEISKTED          DD+DSN
Sbjct: 781 EKDDLVGSANEDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDTSKPEDDDVDSN 840

BLAST of PI0012030 vs. NCBI nr
Match: XP_008464849.1 (PREDICTED: 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like isoform X1 [Cucumis melo] >ADN33992.1 starch branching enzyme [Cucumis melo subsp. melo])

HSP 1 Score: 1672.9 bits (4331), Expect = 0.0e+00
Identity = 807/856 (94.28%), Postives = 823/856 (96.14%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           MLLSPCVLPSTAIASLSLSAKK FISDSRN+WPSLRQQTELNFWSRMSHHRQMNPFIFPY
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHDPGLKPFKDHFKYR+GRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGW+GTNHCMEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKPN PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+M FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNL DT+HLRYKFLNAFDRAMNALDE
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 EKDDLVGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTEDG---------DDIDSN 840
           EKDDLV   +EDV  RH  EDSE LAG EEENDIAVG+ISKTED          DD+DSN
Sbjct: 781 EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDDVDSN 840

Query: 841 EIEDLPVRGTRARIID 847
           +IEDLPVRG RA+I D
Sbjct: 841 KIEDLPVRGNRAKISD 856

BLAST of PI0012030 vs. NCBI nr
Match: XP_038884508.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Benincasa hispida])

HSP 1 Score: 1582.0 bits (4095), Expect = 0.0e+00
Identity = 773/852 (90.73%), Postives = 799/852 (93.78%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           ML SPCVLPS A+ASLSLSAKK F  DSRN+WPSLRQ  ELNF S+MS+HRQMNPF+ P+
Sbjct: 1   MLPSPCVLPSPALASLSLSAKKIFTLDSRNQWPSLRQ--ELNFGSKMSNHRQMNPFMLPF 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYK+VHSPAISSV+TDDT  VSS DES ENIGILSHDPGL+ FKDHFKYR+GRYADLLN
Sbjct: 61  STYKKVHSPAISSVVTDDTPKVSSPDESTENIGILSHDPGLESFKDHFKYRVGRYADLLN 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGWDGTNH MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWDGTNHRMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIKI DL GKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY
Sbjct: 181 FGIWSIKIRDLSGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEFKHPRPAKP APRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINM FTG YNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMAFTGTYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMS LT+ASPVVERGI+LHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVERGISLHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNLPDT+HLRYKFLNAFDRAMN+LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGGHGRVGH +DHFTSPEGIPGV ETNFNNRPNSFKVLSPARTCVVY++VDESKEK
Sbjct: 721 DAWDFGGHGRVGHDVDHFTSPEGIPGVSETNFNNRPNSFKVLSPARTCVVYHRVDESKEK 780

Query: 781 EKDDLVGGANE----DVFVRHSEDSEE-LAGCEEENDIAVGEIS---------KTEDGDD 839
           E D+LV GANE    DVF RH ED  E  AG EEENDIA GEIS         KTEDG D
Sbjct: 781 ENDNLV-GANEARATDVFARHVEDDNEGSAGGEEENDIAAGEISKIEDDTDTRKTEDG-D 840

BLAST of PI0012030 vs. NCBI nr
Match: KAA0038453.1 (1,4-alpha-glucan-branching enzyme 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1552.7 bits (4019), Expect = 0.0e+00
Identity = 746/791 (94.31%), Postives = 760/791 (96.08%), Query Frame = 0

Query: 66  VHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLNLLDKH 125
           VHSPAISSVMTDDTSTVSS DESMENIGILSHDPGLKPFKDHFKYR+GRY DLLNLLDKH
Sbjct: 24  VHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLNLLDKH 83

Query: 126 EGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS 185
           EGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS
Sbjct: 84  EGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNEFGIWS 143

Query: 186 IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPS 245
           IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPP 
Sbjct: 144 IKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPP 203

Query: 246 SERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI 305
            ERYEFKHPRPAKPN PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI
Sbjct: 204 LERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAI 263

Query: 306 MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGL 365
           MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD+VHSHASNNVTDGL
Sbjct: 264 MEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVTDGL 323

Query: 366 NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD 425
           NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD
Sbjct: 324 NGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFD 383

Query: 426 GVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM 485
           GVTSMLYHHHGI+M FTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM
Sbjct: 384 GVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGM 443

Query: 486 PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISYA 545
           PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEKCISYA
Sbjct: 444 PGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEKCISYA 503

Query: 546 ESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGGEGYLN 605
           ESHDQSIVGDKTIAFLLMDKEMYSGMS L NASPVVERGIALHKMIHFITMALGGEGYLN
Sbjct: 504 ESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGGEGYLN 563

Query: 606 FMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFL 665
           FMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNL DT+HLRYKFLNAFDRAMNALDEKFSFL
Sbjct: 564 FMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDEKFSFL 623

Query: 666 ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDF 725
           ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDSDA DF
Sbjct: 624 ASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAGDF 683

Query: 726 GGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL 785
           GG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL
Sbjct: 684 GGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDL 743

Query: 786 VGGANEDVFVRH-SEDSEELAGCEEENDIAVGEISKTEDG---------DDIDSNEIEDL 845
           VG  +EDV  RH  EDSE LAG EEENDIAVG+ISKTED          DD+DSN+IEDL
Sbjct: 744 VGSVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDTSKTEEDDVDSNKIEDL 803

Query: 846 PVRGTRARIID 847
           PVRG RA+I D
Sbjct: 804 PVRGNRAKISD 814

BLAST of PI0012030 vs. NCBI nr
Match: XP_022153149.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Momordica charantia])

HSP 1 Score: 1534.6 bits (3972), Expect = 0.0e+00
Identity = 740/858 (86.25%), Postives = 785/858 (91.49%), Query Frame = 0

Query: 1   MLLSPCVLPSTAIASLSLSAKKFFISDSRNRWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60
           ML S CVLP T++ASLSLSAKK F + SRN+WPSLR+Q EL F SRMSHHR MNPF+ P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRIGRYADLLN 120
           STYKR HS AIS VMTD+  T++S DE MEN+GILSHDPGL+PFKDHFKYRIGRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180
           LL+KHEGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGWDG+NH MEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240
           FGIWSIK+ D GGKPAI+HNSRVKFRFKHGNGVWIDRIPAWI+YATVDPT+FAAPYDGVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP  ERYEF+HPRPAKP APR+YEAHVGMSSSEPRV+SYREFADF+LPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMD++HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+Q SYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNL HSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTNASPVVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKT+AFLLMDKEMYSGMS LT ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDE 660
           EGYLNFMGNEFGHPEWIDFPR+GNGWSYDKCRRQWNLPDT+HLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+S T EEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DALDFGGHGRVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780
           DA DFGGHGR+GH  DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYY+VDESKEK
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESKEK 780

Query: 781 EKDDLVG---GANEDVFVRHSEDSEELAGCEEENDIAVGE------------ISKTEDGD 840
           E  D VG       DV  RH+E+ +E     EE DIAVGE             SK+EDGD
Sbjct: 781 ENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVEDDNIDTSKSEDGD 840

Query: 841 DIDSNEIEDLPVRGTRAR 844
           D D ++ EDLPV+ TRAR
Sbjct: 841 D-DPSKSEDLPVKETRAR 857

BLAST of PI0012030 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 850.9 bits (2197), Expect = 8.8e-247
Identity = 416/710 (58.59%), Postives = 510/710 (71.83%), Query Frame = 0

Query: 98  DPGLKPFKDHFKYRIGRYADLLNLLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 157
           DP L   ++H  YR G+Y  L   +DK+EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIVGDFNGWDGTNHCMEKNEFGIWSIKI-YDLGGKPAISHNSRVKFRFKHGNGVWID 217
           + A ++GDFN W+  +  M +N+FG+W I +  +  G PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEP 277
            IPAWIKY+   P +   PY+GVY+DPP  ++Y FKHPRP KP + R+YE+HVGMSS+EP
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
           ++++Y  F D VLPRIK+  YN VQ+MAI EH+YYASFGYHVTNFFA SSR GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFN 397
           LIDKAH LGL VLMDIVHSHAS N  DGL+ FD    T   YFH+G RGYH +WDSRLFN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGNYNEYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+L H + P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTN 577
            K DE+W +G+I++ LTNRR+ EKC+ YAESHDQ++VGDKTIAF LMDK+MY  M+    
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPVVERGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPRD-----------GNG 637
           A+P V+RGIALHKMI  ITM LGGEGYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFE 697
            SYDKCRR+++L D  +LRY  L  FDRAM  L+E + F+ S  Q +S   E D+VIVFE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHGIDHFTSPEGIP 757
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEKEKDDLV--GGANEDV 794
              +   ++RP SF V +P RT VVY  VD+  + E+  LV  G   EDV
Sbjct: 824 ---DGRHDDRPCSFMVYAPCRTAVVYAAVDDDDDDERSSLVPIGLLPEDV 858

BLAST of PI0012030 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 841.3 bits (2172), Expect = 7.0e-244
Identity = 407/686 (59.33%), Postives = 503/686 (73.32%), Query Frame = 0

Query: 98  DPGLKPFKDHFKYRIGRYADLLNLLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 157
           DP L+ + +H  YR G+Y  L   +DK+EGGL+ F+RGY K GF+R + GI YREWAP A
Sbjct: 129 DPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREWAPGA 188

Query: 158 QEAQIVGDFNGWDGTNHCMEKNEFGIWSIKI-YDLGGKPAISHNSRVKFRFKHGNGVWID 217
           + A ++GDFN W+     M +NEFG+W I +  +  G PAI H SRVK R    +G+  D
Sbjct: 189 KAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSGI-KD 248

Query: 218 RIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHPRPAKPNAPRVYEAHVGMSSSEP 277
            IPAWIK++   P +   P++G+Y+DPP  E+Y FKHP+P +P + R+YEAHVGMSS+EP
Sbjct: 249 SIPAWIKFSVQAPGEI--PFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSSTEP 308

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
            V++Y  F D VLPRIK+  YN VQ+MAI EHSYYASFGYHVTNFFA SSR GTPE+LK 
Sbjct: 309 MVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKS 368

Query: 338 LIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFN 397
           LID+AH LGL VLMDIVHSHAS N  DGLN FD    T   YFH+G RGYH +WDSRLFN
Sbjct: 369 LIDRAHELGLVVLMDIVHSHASKNTLDGLNMFD---GTDAHYFHSGPRGYHWMWDSRLFN 428

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+++GFTGNY EYF   TDVD
Sbjct: 429 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVD 488

Query: 458 AVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLK 517
           AV YLML N++ H + P+A  + EDVSGMP    PV +GG+GFDYRL MAI DKWI+ LK
Sbjct: 489 AVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLK 548

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSSLTN 577
            K DE+W MG+I + LTNRR+SEKCISYAESHDQ++VGDKTIAF LMDK+MY  M+    
Sbjct: 549 -KRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRP 608

Query: 578 ASPVVERGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPR-----------DGNG 637
           ++P+++RGIALHKMI  ITM LGGEGYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 609 STPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIPGNN 668

Query: 638 WSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFE 697
           +SYDKCRR+++L D ++LRY+ L  FD+AM  L+E + F+ S  Q +S   E D+VIVFE
Sbjct: 669 FSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVIVFE 728

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHGRVGHGIDHFTSPEGIP 757
           RGDLVFVFNFH  ++Y  Y++GC  PGKY++ LDSD   FGG  R+    ++FT  +G+ 
Sbjct: 729 RGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFGGFNRLDRKAEYFTY-DGL- 788

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVY 772
                 ++ RP SF V +P RT VVY
Sbjct: 789 ------YDERPCSFMVYAPCRTAVVY 799

BLAST of PI0012030 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 466.5 bits (1199), Expect = 4.7e-131
Identity = 237/582 (40.72%), Postives = 351/582 (60.31%), Query Frame = 0

Query: 195 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHP 254
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P         ++W+P     Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASF 314
           +P  P + R+YE HVG+S SEP+VS++ EF   VLP +K   YN +QL+ + EH  Y + 
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTV 453

Query: 315 GYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQST 374
           GY VTNFFA SSR GTP+D K L+D+AHGLGL V +DIVHS+A+ +   GL+ FD    +
Sbjct: 454 GYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 375 QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHH 434
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 435 HGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFE 494
           +G    F  + ++Y ++  D DA++YL+LAN + H   P+   IAED +  PGL  PV +
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 495 GGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESHDQSIV 554
           GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+QSI 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 555 GDKTIAFLLMDKEMYSGMSSLTNASP----VVERGIALHKMIHFITMALGGEGYLNFMGN 614
           G ++ A +L           + N SP    +++RGI+LHKMI  IT   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL--------FGGVDNGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 615 EFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSK 674
           EFGHPE ++FP   N +S+    R+W+L ++    + F  +FD+ +  LD+    L+   
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGL 813

Query: 675 QIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHG 734
             +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD + +GG G
Sbjct: 814 PSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQG 873

Query: 735 RVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY 772
            V    DH+     +        + + N  +V  P+RT  VY
Sbjct: 874 IVTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 891

BLAST of PI0012030 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 1.1e-127
Identity = 230/554 (41.52%), Postives = 339/554 (61.19%), Query Frame = 0

Query: 195 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKF---AAPYDGVYWDPPSSERYEF 254
           PA+ H S+ +  F   +G  ++R+PAW  Y     T F         ++W+P     Y++
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKW 393

Query: 255 KHPRPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYY 314
           K+ +P  P + R+YE HVG+S SEP+VS++ EF   VLP +K   YN +QL+ + EH  Y
Sbjct: 394 KYSKPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDY 453

Query: 315 ASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVG 374
            + GY VTNFFA SSR GTP+D K L+D+AHGLGL V +DIVHS+A+ +   GL+ FD  
Sbjct: 454 FTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD-- 513

Query: 375 QSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSML 434
             + D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+
Sbjct: 514 -GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMI 573

Query: 435 YHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRP 494
           Y H+G    F  + ++Y ++  D DA++YL+LAN + H   P+   IAED +  PGL  P
Sbjct: 574 YTHNGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEP 633

Query: 495 VFEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESHDQ 554
           V +GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+Q
Sbjct: 634 VSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQ 693

Query: 555 SIVGDKTIAFLLMDKEMYSGMSSLTNASP----VVERGIALHKMIHFITMALGGEGYLNF 614
           SI G ++ A +L           + N SP    +++RGI+LHKMI  IT   GG  YLNF
Sbjct: 694 SISGGRSFAEIL--------FGGVDNGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNF 753

Query: 615 MGNEFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRA--MNALDEKFSF 674
           MGNEFGHPE ++FP   N +S+    R+W+L ++    + F  +FD+   +  LD+    
Sbjct: 754 MGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKVSELMDLDKSKGI 813

Query: 675 LASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALD 734
           L+     +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD + 
Sbjct: 814 LSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVK 870

Query: 735 FGGHGRVGHGIDHF 739
           +GG G V    DH+
Sbjct: 874 YGGQGIVTE--DHY 870

BLAST of PI0012030 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 4.5e-118
Identity = 224/582 (38.49%), Postives = 333/582 (57.22%), Query Frame = 0

Query: 195 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWDPPSSERYEFKHP 254
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P         ++W+P     Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPAKPNAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASF 314
           +P  P + R+YE HVG+S SEP+VS++ EF                              
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTK---------------------------- 453

Query: 315 GYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASNNVTDGLNGFDVGQST 374
              VTNFFA SSR GTP+D K L+D+AHGLGL V +DIVHS+A+ +   GL+ FD    +
Sbjct: 454 --KVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 375 QDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHH 434
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 435 HGINMGFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGRPVFE 494
           +G    F  + ++Y ++  D DA++YL+LAN + H   P+   IAED +  PGL  PV +
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 495 GGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESHDQSIV 554
           GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+QSI 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 555 GDKTIAFLLMDKEMYSGMSSLTNASP----VVERGIALHKMIHFITMALGGEGYLNFMGN 614
           G ++ A +L           + N SP    +++RGI+LHKMI  IT   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL--------FGGVDNGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 615 EFGHPEWIDFPRDGNGWSYDKCRRQWNLPDTNHLRYKFLNAFDRAMNALDEKFSFLASSK 674
           EFGHPE ++FP   N +S+    R+W+L ++    + F  +FD+ +  LD+    L+   
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGL 813

Query: 675 QIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDALDFGGHG 734
             +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD + +GG G
Sbjct: 814 PSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQG 861

Query: 735 RVGHGIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY 772
            V    DH+     +        + + N  +V  P+RT  VY
Sbjct: 874 IVTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 861