PI0011660 (gene) Melon (PI 482460) v1

Overview
NamePI0011660
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionThreonyl-tRNA synthetase
Locationchr01: 3887890 .. 3892074 (-)
RNA-Seq ExpressionPI0011660
SyntenyPI0011660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCCATTTCCCCTAACGTCTCTGCTCCTCGGTTCTTCCTTGCAGTTGCGACCAAATTTTCAAGAAATAGGGACAAATCTTTTTTCCCTCGCAAGAAGTCCAATGGGTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGATTTGGTGGATACCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGAATTCGTCACACGGTGATTCTGTTTTTTCTTTGATTTTCCCCTGTGGGTTTTGGGATAATGTTATTTGAATGTGGGTATGGATTCTTATCTGGGAATTTGAGGACTTTTTATGCTTAATGAAGGGTTTTCTTAATGAGGTTTCCCTGGGTGAAGTTTAGGTTTGAACTAGGCAGGAGTTTCATAACTTCAACAAGGTGTTGCTAACCTAGAAGGGGCTTTCTCAATTCCATTTTGCATCTTGGAGATAAGATTGAATGCGGACTTTAGGTTTCAACTGCTTGATATATGCTTTTCAAATTGCAGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGACGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGTTGGTATTATACCAAATCAATGAGCACTGGTTTTTTTTTCTTTATTATTTTACATCACATGATTTGGAATATATACATTAGTCTATGCTTTTAGGGCGTGGGGTTTATTTCTCATATCCTTTCTAGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGATGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGATTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGTAGGGATTGCAATCATCTTTGGTACTTCTCTGTTCCACCTTTTGAGATCTTCTTGTATTTCATGCTTAGCAATGTTTGATACTTTTCTTGATTGAACACTCGATTCTTTTATCATCTTGCTTTGGCGTATGAATGGCTAGAGTTTAGAGTTTTCTTTTTCTTTTTGTTTCCCAGATTGATATAGATTGGGACTTCACAAGCAAAATATTGAAAAGAAAATACCCCAACGAAAATTAGTATTCATCACAAGATTTTATTCGGCTATACACAATAAGAAGTAAGAGCAGTGTTATGATCGTTGAGTAATATTGGAGATGACTTGAAAACTGCTAAAAAAGATATTGACATAATATTTTTGGCGGGTATAGGATATATTTATTGTTCTTTATTTAACAGAGCTCTCACAGGGCTGGTGAAGTAAAAAGTGCTTATTTAAAGAACAAAAGTATAAAGTGTTCTTGTAAACACCTGAATGTATTTTCTTAATGTGCTTGTAATTTATCTTCCCTTTTCTCTTTCGTGGAGAGATGCTAGGTTGACATAGATGCAGTTTAGGAAATTTTCTTGTTTATTTTCATGATCCTGAATGATTTTCAACGCTTGAAAAGTCATTTCAATTATGTCATTATTTGAAAACTGAAATTGTTCTTTTCTGTGTTATGATCATTGATTAGTATCACTTCGCTGTTAGGATTTTAATGAAAAGAAAGTCTAGAGAGACTCTCCCATTCTTGAAATTAGCAAATTTCTCTCTTTTCTCCATACTCTTCCAAAGTTCAAAATGTTCTCTTGATTGCGATTAAGAGGATATTGGTAGCTGCAAAAGACGGCAAATGGTAGATCAATTGTCTAATAAATTAAGAACGTCTTGGTCTATTTTGATCAGTTGGTAATTCTATCCCTTAACCTTAATTGATGATTAAATTTGTAATGCTTTCTTAGTAGTACCCTATATTCTTCACAGGAAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAAAGGATATACGGCACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAAACGTCGTGATCACAGACGACTTGGGAAAGATCTTGATCTATTTTCCATTCAGGTGTATACTTCCTCCAAAACTATTATAATGTTCTTGATTGTAGCGAGAGTTATGCTCTTAGATTTTGTTGGTCTGAATTGTTTTAGAGCATTGGCTGAACACAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCGAAGGGTGCTGTCGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATCTGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGATGTTGAGAACGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACCTCCTATCAAGATTTTCCTATTAGAGTTGCAGAATTGGGGACGGTATATAGATACGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGCGTCAGAGGTTTTACCCAGGTGCACTTTATGTTGGATGCTGTCATTTTCTTTTTTTGATCATTTAGACTCTACAAGTTGTTATCTGCTCAATGCAGAAAATCAAAATTTCCAAGCTGTGAAGGATTGAAGTTATATTTTGATTGTTTCTCGTTTGTGGCTTAAATAATAAAGATTTTTTGCTGCTTTACTTACACGTTTTATGGGTTTTGCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATTTAACAGAAGAGTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACCAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATTTGCTCTTAAAGATGCTCTAAGTGACAAGGGGTGGGCCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACTATACAGGCAATCCCTCAACTTAATATGGGTTTTTATTGGTAGATCTAATCTTAGTAGAATCTCTTTCACTGTCCTGAAATCTTATGATTTCTACAAACTGATGTTCATGACAGTTTCTTTTGATGTATGAAATAAGCTATCTGACTTGACTGTGGTTAAGTAAAACTGTTATTTCTTTAATATTTAATATTTTATTTCTTTCAGGTTGATTTTAATCTACCTCAGCGGTTTGACATCACATATGTTGATTCAAACTCTGAAAAGAAGAGGCCTATCATGATCCATCGTGCGGTGCTAGGATCTCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGGTATTATATATAAGCATAAAATCTCCAGCAGTTTGTGACATATTAGTTATGTTGTCGCTATAATTTGCTTGGTCCTGTCTTGATTATGTATGAAAGCTTACTGCCAACAAACCCATGCAAGATATTCATCAAGTTCTTAGGTAGAATCATGTCCCTGATGACGAGGTTCATTACGCTTGGAATGGGACCGTGTAAATTTGATTTGCCAACTGAATCAATTGCAGAGATTCATTTTAGGTATTAAAGTAGTGATATTCACTGAAATTTCCATTGGTTTTCTATCCCAGCTCTCATACTGCAAAGAGGTGACTGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTCTGTCATGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAATCAAATTTGCCTCAGAGAATAGAACGCCATTTTGA

mRNA sequence

ATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCCATTTCCCCTAACGTCTCTGCTCCTCGGTTCTTCCTTGCAGTTGCGACCAAATTTTCAAGAAATAGGGACAAATCTTTTTTCCCTCGCAAGAAGTCCAATGGGTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGATTTGGTGGATACCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGAATTCGTCACACGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGACGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGATGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGATTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGAAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAAAGGATATACGGCACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAAACGTCGTGATCACAGACGACTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCGAAGGGTGCTGTCGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATCTGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGATGTTGAGAACGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACCTCCTATCAAGATTTTCCTATTAGAGTTGCAGAATTGGGGACGGTATATAGATACGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGCGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATTTAACAGAAGAGTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACCAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATTTGCTCTTAAAGATGCTCTAAGTGACAAGGGGTGGGCCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACTATACAGGTTGATTTTAATCTACCTCAGCGGTTTGACATCACATATGTTGATTCAAACTCTGAAAAGAAGAGGCCTATCATGATCCATCGTGCGGTGCTAGGATCTCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGCTCTCATACTGCAAAGAGGTGACTGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTCTGTCATGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAATCAAATTTGCCTCAGAGAATAGAACGCCATTTTGA

Coding sequence (CDS)

ATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCCATTTCCCCTAACGTCTCTGCTCCTCGGTTCTTCCTTGCAGTTGCGACCAAATTTTCAAGAAATAGGGACAAATCTTTTTTCCCTCGCAAGAAGTCCAATGGGTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGATTTGGTGGATACCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGAATTCGTCACACGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGACGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGATATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGATGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGATTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGAAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGATGAGAAGAATCCAATGCTACAAAGGATATACGGCACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAAACGTCGTGATCACAGACGACTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCGAAGGGTGCTGTCGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATCTGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGATGTTGAGAACGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACCTCCTATCAAGATTTTCCTATTAGAGTTGCAGAATTGGGGACGGTATATAGATACGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGCGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATTTAACAGAAGAGTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACCAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATTTGCTCTTAAAGATGCTCTAAGTGACAAGGGGTGGGCCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACTATACAGGTTGATTTTAATCTACCTCAGCGGTTTGACATCACATATGTTGATTCAAACTCTGAAAAGAAGAGGCCTATCATGATCCATCGTGCGGTGCTAGGATCTCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGCTCTCATACTGCAAAGAGGTGACTGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTCTGTCATGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAATCAAATTTGCCTCAGAGAATAGAACGCCATTTTGA

Protein sequence

MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTSISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF
Homology
BLAST of PI0011660 vs. ExPASy Swiss-Prot
Match: F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 2.5e-310
Identity = 514/664 (77.41%), Postives = 588/664 (88.55%), Query Frame = 0

Query: 7   MATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTSISVATD 66
           MA++  LL  S  ++  S   S+ R F+ + T       + F+PR++ +GFST  +VAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60

Query: 67  SSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
              P+IS+         K +   +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  ---PAISSSG-----PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
           VR+IIE+ WKK+H E GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWA 486
           QIKDEIRGVLDLTEE+L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW+
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540

Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEV 606
           RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEV++KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600

Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666
           CHGERLPKLIRNAETQKIPLMAVVG KEVE  TVTVRSRFGGE+GTI VDD + +I  A 
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648

Query: 667 ENRT 671
           E RT
Sbjct: 661 ETRT 648

BLAST of PI0011660 vs. ExPASy Swiss-Prot
Match: Q0I902 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 5.6e-225
Identity = 365/582 (62.71%), Postives = 456/582 (78.35%), Query Frame = 0

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           +VLP    SE LL+IRH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSR+EA+RRI+A NEPYK+EILE + E PIT+Y +G +
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE    LQRIYGTAWET +QL  +   
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 329
           KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY+LLY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +L+LTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDA 509
           + +E+NLST+P K++G+D +WE AT  L +AL  KGWAY+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P Q+R+LPVTD  L Y +E   +L+A+G+RA +   G+RL KLIR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIRIGEKMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFASENR 670
           AV+GAKE E    ++RSR  G++G IT +  +   + A+++R
Sbjct: 563 AVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603

BLAST of PI0011660 vs. ExPASy Swiss-Prot
Match: A5GJT7 (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.1e-224
Identity = 364/582 (62.54%), Postives = 457/582 (78.52%), Query Frame = 0

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           +VLP    +E+LLRIRH+ +HVMAMAVQ+LFP+A+VTIGPW E+GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSENEQLLRIRHSMSHVMAMAVQQLFPNARVTIGPWTESGFYYDFDNPEPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKK M +II++ LPL R EVSR EA+ +I+A NEPYK+EILE ++E PIT+Y +G +
Sbjct: 83  LKAIKKGMIKIINKKLPLERVEVSRSEAEEKIKAQNEPYKLEILEGLQE-PITLYTLGED 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHVE+TG++N +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTGQLNAKAFELESVAGAYWRGDETKAQLQRIYGTAWETAEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 329
           KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY+LLY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEEFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDA 509
           N +E+NLSTRP KS+G+D +W+ AT  L +AL  KGWAY+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NTYEINLSTRPEKSIGDDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF + Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFKLDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           PLWL+P QVR+LPVTD    Y + + ++L  +GVRA +   G+RL KLIR  E  KIP++
Sbjct: 503 PLWLAPEQVRLLPVTDEVQPYAESLLDQLTQAGVRATIDRSGDRLGKLIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFASENR 670
           AV+GAKE E   V++RSR  G++G + V D +   + A+  R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVVAVADLLRAAQNANSQR 603

BLAST of PI0011660 vs. ExPASy Swiss-Prot
Match: Q3AYC6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.1e-223
Identity = 364/584 (62.33%), Postives = 455/584 (77.91%), Query Frame = 0

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           +VLP    SE+LL+IRH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSRDEA+ RI+A NEPYK+EILE ++E PIT+Y +G++
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 329
           K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY+LLY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL DQI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDA 509
           N +E+NLSTRP KS+G++ +WE AT  L +AL  KGW ++IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF + YV ++  K++PIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P Q+R+LPVTD    Y ++V  +L+ +G+RA +   G+RL K+IR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFASENRTP 672
           AV+GAKE E   +++RSR  G++G  +VD  +   + A+  R P
Sbjct: 563 AVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAERQP 605

BLAST of PI0011660 vs. ExPASy Swiss-Prot
Match: Q3AKU0 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 9.9e-222
Identity = 357/582 (61.34%), Postives = 453/582 (77.84%), Query Frame = 0

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 149
           +VLP    S++LL+IRH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD  +P  + D
Sbjct: 23  VVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDNPDPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 209
           LK IKK M +II++ LPL R EVSR+EA+ +I+A NEPYK+EIL+ + E PIT+Y +G +
Sbjct: 83  LKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PITLYTLGED 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE    LQRIYGTAWE+ EQL  +   
Sbjct: 143 WWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 329
           KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY+LLY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSAFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDA 509
           + +E+NLSTRP KS+G D +W+ AT  L +AL  KGWAY+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P QVR+LPVTD    Y +++ ++L  +GVRA V   G+RL KLIR  E  KIP++
Sbjct: 503 PFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSGDRLGKLIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFASENR 670
           AV+GAKE E   V++RSR  G++G   V D +   + A+  R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 603

BLAST of PI0011660 vs. ExPASy TrEMBL
Match: A0A0A0K179 (Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/672 (97.77%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAV+TKF+RNRD+ F PR KSNGFSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPSISTQNEDLVD HKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKME+LESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYD+LYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTE+LLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEV+EKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTI VDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. ExPASy TrEMBL
Match: A0A1S3CHN3 (Threonyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103500535 PE=3 SV=1)

HSP 1 Score: 1337.8 bits (3461), Expect = 0.0e+00
Identity = 655/672 (97.47%), Postives = 663/672 (98.66%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAV+TKF+ NRD+SFFPRKKSN FSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPS+STQNEDLVD HKDQT KIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSVSTQNEDLVDAHKDQTGKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEV+EKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVCH ERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG
Sbjct: 601 GVRAEVCHSERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. ExPASy TrEMBL
Match: A0A5A7V0A9 (Threonyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00590 PE=3 SV=1)

HSP 1 Score: 1330.1 bits (3441), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 664/694 (95.68%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAV+TKF+ NRD+SFFPRKKSN FSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPS+STQNED VD HKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSVSTQNEDFVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDA--------------- 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDA               
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQRFILGIKVVMFFTE 600

Query: 601 -------QLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ 660
                  QLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ
Sbjct: 601 IFIAFLSQLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ 660

Query: 661 TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF 673
           TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF
Sbjct: 661 TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF 694

BLAST of PI0011660 vs. ExPASy TrEMBL
Match: A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 641/672 (95.39%), Postives = 655/672 (97.47%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMA+TTGLLLH PLV PISPNVS PRFFLAVAT+F+ NRDKS F RKK+NGFSTS
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           I+VATDSSAPSI TQNEDLVDTHK+++EKIVLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVE+E
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSL GLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFN+FEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYV+SNSEKK
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQL YCKEVTEKLRA 
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAI 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVC GERLPKLIRNAETQKIPLMAVVGAKEVE +TVTVRSRFGGEIGTITVDDF G
Sbjct: 601 GVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. ExPASy TrEMBL
Match: A0A6J1HZS8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1)

HSP 1 Score: 1303.5 bits (3372), Expect = 0.0e+00
Identity = 640/672 (95.24%), Postives = 653/672 (97.17%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMA+TTGLLLH PLV PISPNVSAPRFFLAVAT+F+ NRDKS F RKK+NGFSTS
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSAPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           I+VATDSSAPSI TQNEDLVDTHK+++EKIVLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSR EAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRGEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKN MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNQMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWK LHRERGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVE+E
Sbjct: 301 HPKGAVVRSIIEDLWKTLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSL GLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFN+FEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYV+SNSEKK
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQL YCKEVTEKLRA 
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAI 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVC GERLPKLIRNAETQKIPLMAVVGAKEVE +TVTVRSRFGGEIGTITVDDF G
Sbjct: 601 GVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. NCBI nr
Match: XP_004144536.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN43435.1 hypothetical protein Csa_020587 [Cucumis sativus])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/672 (97.77%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAV+TKF+RNRD+ F PR KSNGFSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPSISTQNEDLVD HKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKME+LESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYD+LYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTE+LLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEV+EKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTI VDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. NCBI nr
Match: XP_008462105.1 (PREDICTED: threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis melo])

HSP 1 Score: 1337.8 bits (3461), Expect = 0.0e+00
Identity = 655/672 (97.47%), Postives = 663/672 (98.66%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAV+TKF+ NRD+SFFPRKKSN FSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPS+STQNEDLVD HKDQT KIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSVSTQNEDLVDAHKDQTGKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEV+EKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVCH ERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG
Sbjct: 601 GVRAEVCHSERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. NCBI nr
Match: KAA0059181.1 (threonine--tRNA ligase [Cucumis melo var. makuwa] >TYK19338.1 threonine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1330.1 bits (3441), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 664/694 (95.68%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAV+TKF+ NRD+SFFPRKKSN FSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPS+STQNED VD HKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSVSTQNEDFVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
            DKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDA--------------- 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDA               
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQRFILGIKVVMFFTE 600

Query: 601 -------QLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ 660
                  QLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ
Sbjct: 601 IFIAFLSQLSYCKEVTEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQ 660

Query: 661 TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF 673
           TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF
Sbjct: 661 TVTVRSRFGGEIGTITVDDFVGRIKFASENRTPF 694

BLAST of PI0011660 vs. NCBI nr
Match: XP_038888474.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Benincasa hispida])

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 647/672 (96.28%), Postives = 659/672 (98.07%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMATTTGLLLH PLV P SPNVSAPRFFL VAT+F+ NRDKS FPRKKSNGFSTS
Sbjct: 1   MFVLQRMATTTGLLLHPPLVRPTSPNVSAPRFFLTVATQFAGNRDKSLFPRKKSNGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           I+VATDSSAPSISTQNEDLVDTH+DQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSISTQNEDLVDTHEDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVE+E
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSL GLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFN+FEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           SDKGW YQID+GGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYV+SNSEK+
Sbjct: 481 SDKGWGYQIDDGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKR 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQ+SYCKEV EKLRA 
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQISYCKEVAEKLRAI 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           G+RAEVC GERLPKLIRNAETQKIPLMAVVGAKEVE QTVTVRSRFGGEIGTITVDDFVG
Sbjct: 601 GIRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIK+ASENRTPF
Sbjct: 661 RIKYASENRTPF 672

BLAST of PI0011660 vs. NCBI nr
Match: XP_022952560.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata])

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 641/672 (95.39%), Postives = 655/672 (97.47%), Query Frame = 0

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTS 60
           MFVLQRMA+TTGLLLH PLV PISPNVS PRFFLAVAT+F+ NRDKS F RKK+NGFSTS
Sbjct: 1   MFVLQRMASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           I+VATDSSAPSI TQNEDLVDTHK+++EKIVLPTNESSEKLLRIRH+CAHVMAMAVQKLF
Sbjct: 61  IAVATDSSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKA LWKTSGHSDFY ENMYNQMDVE+E
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSL GLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDAL 480
           IFCLDDQIKDEIRGVLDLTEELLLQFGFN+FEVNLSTRPPKSVGNDEIWEKAT ALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDAL 480

Query: 481 SDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           SDKGW YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYV+SNSEKK
Sbjct: 481 SDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQL YCKEVTEKLRA 
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAI 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
           GVRAEVC GERLPKLIRNAETQKIPLMAVVGAKEVE +TVTVRSRFGGEIGTITVDDF G
Sbjct: 601 GVRAEVCQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of PI0011660 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 1065.4 bits (2754), Expect = 1.8e-311
Identity = 514/664 (77.41%), Postives = 588/664 (88.55%), Query Frame = 0

Query: 7   MATTTGLLLHSPLVTPISPNVSAPRFFLAVATKFSRNRDKSFFPRKKSNGFSTSISVATD 66
           MA++  LL  S  ++  S   S+ R F+ + T       + F+PR++ +GFST  +VAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60

Query: 67  SSAPSISTQNEDLVDTHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
              P+IS+         K +   +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  ---PAISSSG-----PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
           VR+IIE+ WKK+H E GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWA 486
           QIKDEIRGVLDLTEE+L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW+
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540

Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEV 606
           RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEV++KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600

Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666
           CHGERLPKLIRNAETQKIPLMAVVG KEVE  TVTVRSRFGGE+GTI VDD + +I  A 
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648

Query: 667 ENRT 671
           E RT
Sbjct: 661 ETRT 648

BLAST of PI0011660 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 408.7 bits (1049), Expect = 9.2e-114
Identity = 219/565 (38.76%), Postives = 337/565 (59.65%), Query Frame = 0

Query: 106 HTCAHVMAMAVQKLFPDAKVTIGPWIE--NGFYYDFDMEPLG--DKDLKKIKKEMERIIS 165
           H+ AH++  A+++ +   ++ IGP      GFYYD     LG  D     I+    +   
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206

Query: 166 RNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKED-PITIYHIGNEWWDLCAGPHVES 225
              P  R EV++D+A       N  +K+E++  +  D  IT+Y  G    DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266

Query: 226 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRL 285
           T  +  +A +    + AYW+GD+    LQR+YG ++  ++QLK YL F EEAK+ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326

Query: 286 GKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKT 345
           G+  +LF        G  F+ P G  V + + D  K  +  RGY  + TP++   ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386

Query: 346 SGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 405
           SGH+D Y +NM+   ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446

Query: 406 LSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPP 465
            SG+L GL RVR F QDDAHIFC  +Q+K E++GVL+  + +   FGF  +E+ LSTRP 
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506

Query: 466 KSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQV 525
           K +G+ E W+KA   LK+A+   G    ++EG GAFYGPKIDI + DA+ RK+QC+T+Q+
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATLQL 566

Query: 526 DFNLPQRFDITY-VDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRV 585
           DF LP RF++ Y  +  ++K RP+MIHRAVLGS+ER F IL+E+Y G +P W+SP Q  V
Sbjct: 567 DFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIV 626

Query: 586 LPVTDAQLSYCKEVTEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEMQ 645
            P+++    Y ++V ++++ +G   +    +R + K +R A+  +   + VVG  E    
Sbjct: 627 CPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATG 686

Query: 646 TVTVRSRFGGEIGTITVDDFVGRIK 664
            V+VR R        +++D +   K
Sbjct: 687 QVSVRVRDNAAHSVKSIEDLLEEFK 702

BLAST of PI0011660 vs. TAIR 10
Match: AT1G17960.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 240.4 bits (612), Expect = 4.3e-63
Identity = 127/287 (44.25%), Postives = 178/287 (62.02%), Query Frame = 0

Query: 387 PIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQF 446
           PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC  DQ+++E++GVLD  + +   F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230

Query: 447 GFNEFEVNLSTRPPKSVGNDEIWEKATFALKDALSDKGWAYQIDEGGGAFYGPKIDIKIE 506
           GF  +E+ LSTRP   +G+ E W KA   L+ AL D G  + I EG GAFYGPKIDI + 
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290

Query: 507 DALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAG 566
           DA  RK+QC+TIQ+DF LP  F + Y+   +E + P+MIH AVLGS+ER F IL+E+Y G
Sbjct: 291 DARNRKFQCATIQLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERMFAILLEHYKG 350

Query: 567 DFPLWLSPIQVRVLPVTDAQLSYCKEVTEKLRASGVRAEVCHGER-LPKLIRNAETQKIP 626
            +P WLSP Q  V  +++   SY K+V +++   G   ++   +R L K + +A      
Sbjct: 351 IWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYN 410

Query: 627 LMAVVGAKEVEMQTVTVRSRFGGE----IGTITVDDFVGRIKFASEN 669
            + VVG KEV    VTVR R   E    +  ++++  +   KF + N
Sbjct: 411 YILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456

BLAST of PI0011660 vs. TAIR 10
Match: AT1G18130.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.6e-15
Identity = 47/125 (37.60%), Postives = 69/125 (55.20%), Query Frame = 0

Query: 520 VDFNLPQRFDITYVDSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVR 579
           VDF+L  RF + Y   N    +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q  
Sbjct: 17  VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76

Query: 580 VLPVTDAQLSYCKEVTEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEM 639
           V  V++   SY  +V +++  +G   +V   +R +   ++ A+      + VVG +E   
Sbjct: 77  VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136

Query: 640 QTVTV 643
             VTV
Sbjct: 137 GQVTV 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IFC52.5e-31077.41Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX... [more]
Q0I9025.6e-22562.71Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=... [more]
A5GJT72.1e-22462.54Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=... [more]
Q3AYC61.1e-22362.33Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE... [more]
Q3AKU09.9e-22261.34Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE... [more]
Match NameE-valueIdentityDescription
A0A0A0K1790.0e+0097.77Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1[more]
A0A1S3CHN30.0e+0097.47Threonyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103500535 PE=3 SV=1[more]
A0A5A7V0A90.0e+0094.67Threonyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1GM450.0e+0095.39Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1[more]
A0A6J1HZS80.0e+0095.24Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004144536.10.0e+0097.77threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN4343... [more]
XP_008462105.10.0e+0097.47PREDICTED: threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis melo][more]
KAA0059181.10.0e+0094.67threonine--tRNA ligase [Cucumis melo var. makuwa] >TYK19338.1 threonine--tRNA li... [more]
XP_038888474.10.0e+0096.28threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Benincasa hispida][more]
XP_022952560.10.0e+0095.39threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G04842.11.8e-31177.41threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative [more]
AT5G26830.19.2e-11438.76Threonyl-tRNA synthetase [more]
AT1G17960.14.3e-6344.25Threonyl-tRNA synthetase [more]
AT1G18130.12.6e-1537.60Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002320Threonine-tRNA ligase, class IIaPRINTSPR01047TRNASYNTHTHRcoord: 569..581
score: 60.71
coord: 397..420
score: 65.62
coord: 364..392
score: 46.55
coord: 499..527
score: 57.02
coord: 542..555
score: 68.62
IPR002320Threonine-tRNA ligase, class IIaTIGRFAMTIGR00418TIGR00418coord: 105..663
e-value: 1.8E-219
score: 728.3
IPR002320Threonine-tRNA ligase, class IIaPANTHERPTHR11451THREONINE-TRNA LIGASEcoord: 63..670
IPR002320Threonine-tRNA ligase, class IIaHAMAPMF_00184Thr_tRNA_synthcoord: 99..668
score: 33.294922
IPR012947Threonyl/alanyl tRNA synthetase, SADSMARTSM00863tRNA_SAD_4coord: 200..252
e-value: 2.4E-21
score: 86.9
IPR012947Threonyl/alanyl tRNA synthetase, SADPFAMPF07973tRNA_SADcoord: 200..252
e-value: 3.2E-13
score: 49.5
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 258..567
e-value: 1.2E-121
score: 407.6
NoneNo IPR availableGENE3D3.30.54.20coord: 162..218
e-value: 1.9E-60
score: 205.7
NoneNo IPR availableGENE3D3.30.980.10coord: 103..256
e-value: 1.9E-60
score: 205.7
NoneNo IPR availablePANTHERPTHR11451:SF44THREONINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIAL 2coord: 63..670
NoneNo IPR availableCDDcd00860ThrRS_anticodoncoord: 574..663
e-value: 2.08243E-33
score: 121.072
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 562..663
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 276..561
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 568..671
e-value: 2.3E-27
score: 97.4
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 356..564
e-value: 6.6E-41
score: 140.2
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 576..663
e-value: 1.9E-18
score: 66.4
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 300..569
score: 25.904842
IPR033728Threonine-tRNA ligase catalytic core domainCDDcd00771ThrRS_corecoord: 276..574
e-value: 0.0
score: 513.636
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamilySUPERFAMILY55186ThrRS/AlaRS common domaincoord: 97..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0011660.1PI0011660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006435 threonyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004829 threonine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding