PI0011427 (gene) Melon (PI 482460) v1

Overview


Name	PI0011427
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Mitotic checkpoint protein BUB3.3
Location	chr09: 15839204 .. 15839731 (-)
RNA-Seq Expression	PI0011427
Synteny	PI0011427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTATCTGGATTTAATTTAATTGTGGCTTCTGGAGCATGTGTATACTTATTAGACTTGCGTAACCTAGAGAAATCAATTCAACTGAAAGATTCTTATATGGAAGCTCCAGTAGCTTGTGTTCGCTCAGTTCCTTATAGTGAAGGTAAGTTATTTTACTTGGCATAATGGCATGTTATATATACATCACTGATCCTTTTTGTGTTATACTGTTATGTTACTTTGAAAACATGGATTATGGGGTCATATCGCTTCTTTCATATAAATGTATTTGCAGTTTATTCCAGATAAAATTTTTGGCTGATATTGATCTTTGAGGATGACCCTCTTTCTTTAATTTATTTTTTTTCCAGTAATATTTACTTTTTTTAAAACTATATAACATCAAGACATTGTTTGCTTGAATTGTATTGACCACCTCTAGATACTATTGTTGCAGGAGTTGCGGTTGGATCAGTAGATGGATGTGTAGCTTTGGATATTGCTTGTCTAGACCAAACTGATAACATTAGGTTTTTATTATAA

mRNA sequence

ATGTCTCTATCTGGATTTAATTTAATTGTGGCTTCTGGAGCATGTGTATACTTATTAGACTTGCGTAACCTAGAGAAATCAATTCAACTGAAAGATTCTTATATGGAAGCTCCAGTAGCTTGTGTTCGCTCAGTTCCTTATAGTGAAGGAGTTGCGGTTGGATCAGTAGATGGATGTGTAGCTTTGGATATTGCTTGTCTAGACCAAACTGATAACATTAGGTTTTTATTATAA

Coding sequence (CDS)

ATGTCTCTATCTGGATTTAATTTAATTGTGGCTTCTGGAGCATGTGTATACTTATTAGACTTGCGTAACCTAGAGAAATCAATTCAACTGAAAGATTCTTATATGGAAGCTCCAGTAGCTTGTGTTCGCTCAGTTCCTTATAGTGAAGGAGTTGCGGTTGGATCAGTAGATGGATGTGTAGCTTTGGATATTGCTTGTCTAGACCAAACTGATAACATTAGGTTTTTATTATAA

Protein sequence

MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCVALDIACLDQTDNIRFLL

Homology

[Close]

BLAST of PI0011427 vs. ExPASy Swiss-Prot
Match: F4I241 (Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.8e-11
Identity = 33/75 (44.00%), Postives = 50/75 (66.67%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           +++SG NL+V   A +++ DLRNL+++ Q   S +E P+ C+ SVPYS G AVGSVDG V
Sbjct: 142 VTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITSVPYSRGYAVGSVDGRV 201

Query: 61  ALDIACLDQTDNIRF 76
           A+D      +  I++
Sbjct: 202 AVDFPNTSCSSEIKY 216

[Close]

BLAST of PI0011427 vs. ExPASy Swiss-Prot
Match: Q9LJN8 (Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 1.9e-04
Identity = 27/70 (38.57%), Postives = 41/70 (58.57%), Query Frame = 0


Query: 1   MSLSGFNLIVA-SGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGC 60
           MSL G  L+VA +G  V + DLRN+ +  Q ++S ++    CVR  P   G A+ SV+G 
Sbjct: 150 MSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTRCVRCYPNGTGYALSSVEGR 209

Query: 61  VALDIACLDQ 70
           VA++   L +
Sbjct: 210 VAMEFFDLSE 219

[Close]

BLAST of PI0011427 vs. ExPASy TrEMBL
Match: A0A1S3CPL0 (mitotic checkpoint protein BUB3.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503359 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.8e-29
Identity = 69/75 (92.00%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDG V
Sbjct: 64  MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGRV 123

Query: 61  ALDIACLDQTDNIRF 76
           ALDIACLDQTD+IR+
Sbjct: 124 ALDIACLDQTDDIRY 138

[Close]

BLAST of PI0011427 vs. ExPASy TrEMBL
Match: A0A1S4E5H8 (mitotic checkpoint protein BUB3.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503359 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.8e-29
Identity = 69/75 (92.00%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDG V
Sbjct: 38  MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGRV 97

Query: 61  ALDIACLDQTDNIRF 76
           ALDIACLDQTD+IR+
Sbjct: 98  ALDIACLDQTDDIRY 112

[Close]

BLAST of PI0011427 vs. ExPASy TrEMBL
Match: A0A0A0KNE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.0e-29
Identity = 68/76 (89.47%), Postives = 72/76 (94.74%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPY EGVAVGSVDG V
Sbjct: 144 MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYREGVAVGSVDGRV 203

Query: 61  ALDIACLDQTDNIRFL 77
           ALDIACLDQTD+IR++
Sbjct: 204 ALDIACLDQTDDIRYI 219

[Close]

BLAST of PI0011427 vs. ExPASy TrEMBL
Match: A0A5D3DCW9 (Mitotic checkpoint protein BUB3.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold518G00020 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.9e-29
Identity = 69/77 (89.61%), Postives = 72/77 (93.51%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDG V
Sbjct: 144 MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGRV 203

Query: 61  ALDIACLDQTDNIRFLL 78
           ALDIACLDQTD+I  +L
Sbjct: 204 ALDIACLDQTDDISNIL 220

[Close]

BLAST of PI0011427 vs. ExPASy TrEMBL
Match: A0A6J1I8A9 (mitotic checkpoint protein BUB3.3 OS=Cucurbita maxima OX=3661 GN=LOC111471870 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.5e-23
Identity = 56/76 (73.68%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MS+SGFNLIVASG CVY LDLRN++K +QL DS M+ PVACV SVPY+EG AVGSVDG V
Sbjct: 144 MSISGFNLIVASGVCVYSLDLRNIQKPVQLTDSCMKVPVACVSSVPYTEGFAVGSVDGRV 203

Query: 61  ALDIACLDQTDNIRFL 77
           AL+IACLDQ D+IR++
Sbjct: 204 ALEIACLDQADDIRYI 219

[Close]

BLAST of PI0011427 vs. NCBI nr
Match: XP_008465750.1 (PREDICTED: mitotic checkpoint protein BUB3.3 isoform X1 [Cucumis melo])

HSP 1 Score: 137.9 bits (346), Expect = 3.7e-29
Identity = 69/75 (92.00%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDG V
Sbjct: 64  MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGRV 123

Query: 61  ALDIACLDQTDNIRF 76
           ALDIACLDQTD+IR+
Sbjct: 124 ALDIACLDQTDDIRY 138

[Close]

BLAST of PI0011427 vs. NCBI nr
Match: XP_016903479.1 (PREDICTED: mitotic checkpoint protein BUB3.3 isoform X2 [Cucumis melo])

HSP 1 Score: 137.9 bits (346), Expect = 3.7e-29
Identity = 69/75 (92.00%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDG V
Sbjct: 38  MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGRV 97

Query: 61  ALDIACLDQTDNIRF 76
           ALDIACLDQTD+IR+
Sbjct: 98  ALDIACLDQTDDIRY 112

[Close]

BLAST of PI0011427 vs. NCBI nr
Match: XP_031740855.1 (mitotic checkpoint protein BUB3.3 isoform X2 [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 8.3e-29
Identity = 68/76 (89.47%), Postives = 72/76 (94.74%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPY EGVAVGSVDG V
Sbjct: 144 MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYREGVAVGSVDGRV 203

Query: 61  ALDIACLDQTDNIRFL 77
           ALDIACLDQTD+IR++
Sbjct: 204 ALDIACLDQTDDIRYI 219

[Close]

BLAST of PI0011427 vs. NCBI nr
Match: XP_031740854.1 (mitotic checkpoint protein BUB3.3 isoform X1 [Cucumis sativus] >KGN51155.1 hypothetical protein Csa_009057 [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 8.3e-29
Identity = 68/76 (89.47%), Postives = 72/76 (94.74%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPY EGVAVGSVDG V
Sbjct: 144 MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYREGVAVGSVDGRV 203

Query: 61  ALDIACLDQTDNIRFL 77
           ALDIACLDQTD+IR++
Sbjct: 204 ALDIACLDQTDDIRYI 219

[Close]

BLAST of PI0011427 vs. NCBI nr
Match: XP_038889965.1 (mitotic checkpoint protein BUB3.3 isoform X2 [Benincasa hispida])

HSP 1 Score: 136.0 bits (341), Expect = 1.4e-28
Identity = 67/76 (88.16%), Postives = 71/76 (93.42%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           MSLSGFNL VAS ACVYLLDLRNLEKSIQLKDSYM+ PVACV SVPYSEGVAVGSVDGCV
Sbjct: 152 MSLSGFNLNVASRACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDGCV 211

Query: 61  ALDIACLDQTDNIRFL 77
           ALDI CLDQTD+IR++
Sbjct: 212 ALDITCLDQTDDIRYI 227

[Close]

BLAST of PI0011427 vs. TAIR 10
Match: AT1G69400.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 2.0e-12
Identity = 33/75 (44.00%), Postives = 50/75 (66.67%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           +++SG NL+V   A +++ DLRNL+++ Q   S +E P+ C+ SVPYS G AVGSVDG V
Sbjct: 142 VTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITSVPYSRGYAVGSVDGRV 201

Query: 61  ALDIACLDQTDNIRF 76
           A+D      +  I++
Sbjct: 202 AVDFPNTSCSSEIKY 216

[Close]

BLAST of PI0011427 vs. TAIR 10
Match: AT1G69400.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 2.0e-12
Identity = 33/75 (44.00%), Postives = 50/75 (66.67%), Query Frame = 0


Query: 1   MSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGCV 60
           +++SG NL+V   A +++ DLRNL+++ Q   S +E P+ C+ SVPYS G AVGSVDG V
Sbjct: 142 VTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITSVPYSRGYAVGSVDGRV 201

Query: 61  ALDIACLDQTDNIRF 76
           A+D      +  I++
Sbjct: 202 AVDFPNTSCSSEIKY 216

[Close]

BLAST of PI0011427 vs. TAIR 10
Match: AT3G19590.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.4e-05
Identity = 27/70 (38.57%), Postives = 41/70 (58.57%), Query Frame = 0


Query: 1   MSLSGFNLIVA-SGACVYLLDLRNLEKSIQLKDSYMEAPVACVRSVPYSEGVAVGSVDGC 60
           MSL G  L+VA +G  V + DLRN+ +  Q ++S ++    CVR  P   G A+ SV+G 
Sbjct: 150 MSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTRCVRCYPNGTGYALSSVEGR 209

Query: 61  VALDIACLDQ 70
           VA++   L +
Sbjct: 210 VAMEFFDLSE 219

The following BLAST results are available for this feature:

BLAST of PI0011427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I241	2.8e-11	44.00	Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2...	[more]
Q9LJN8	1.9e-04	38.57	Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1...	[more]

BLAST of PI0011427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CPL0	1.8e-29	92.00	mitotic checkpoint protein BUB3.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A1S4E5H8	1.8e-29	92.00	mitotic checkpoint protein BUB3.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A0A0KNE2	4.0e-29	89.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1	[more]
A0A5D3DCW9	8.9e-29	89.61	Mitotic checkpoint protein BUB3.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1I8A9	2.5e-23	73.68	mitotic checkpoint protein BUB3.3 OS=Cucurbita maxima OX=3661 GN=LOC111471870 PE...	[more]

BLAST of PI0011427 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465750.1	3.7e-29	92.00	PREDICTED: mitotic checkpoint protein BUB3.3 isoform X1 [Cucumis melo]	[more]
XP_016903479.1	3.7e-29	92.00	PREDICTED: mitotic checkpoint protein BUB3.3 isoform X2 [Cucumis melo]	[more]
XP_031740855.1	8.3e-29	89.47	mitotic checkpoint protein BUB3.3 isoform X2 [Cucumis sativus]	[more]
XP_031740854.1	8.3e-29	89.47	mitotic checkpoint protein BUB3.3 isoform X1 [Cucumis sativus] >KGN51155.1 hypot...	[more]
XP_038889965.1	1.4e-28	88.16	mitotic checkpoint protein BUB3.3 isoform X2 [Benincasa hispida]	[more]

BLAST of PI0011427 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69400.2	2.0e-12	44.00	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G69400.1	2.0e-12	44.00	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G19590.1	1.4e-05	38.57	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 1..76 e-value: 2.8E-6 score: 28.5
None	No IPR available	PANTHER	PTHR10971	MRNA EXPORT FACTOR AND BUB3	coord: 1..74
None	No IPR available	PANTHER	PTHR10971:SF17	MITOTIC CHECKPOINT PROTEIN BUB3.3	coord: 1..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0011427.1	PI0011427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045727	positive regulation of translation
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003723	RNA binding