PI0011215 (gene) Melon (PI 482460) v1

Overview
NamePI0011215
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionchaperone protein DnaJ
Locationchr07: 19846693 .. 19849164 (-)
RNA-Seq ExpressionPI0011215
SyntenyPI0011215
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCATTCATGGTGGATCTTCCTCAAGCTCTGAGCATCTCCTTTCGAAATTTCTGCAAATCCTACAAATCCTTCTTCAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGGATACCGATAATGTCGTTGTTGATACTAAACACGACGACGCTACCTCCGACGACATCCATAATAATGGACCTTCCAAGGTATATATATATATATATATATATGTGCTTTCAAACGTTTTTATGATTAATCAGATCGACCCTTGTTATTTCTGTTCGTGTTTTTCTTTGATTACAGATTTTTTTATTTTGGTCTCTACGCCTGATGGAAAGGATGGAGAAGCCATAATTTATTAGGCGTTTAATCGAAAATAATCGTTGATTTTCAGGATCATCATGAAGAAGTTAAGAAAAAGGAAGGACCGAGACCGACGACGACAAAAGGCGTTCATAGCTTTAGATATGGAGGTCGGAGTCTGAGAGAGAATGATACGACGAGTTTCAGGCCTCGAAGCTATGACAGTGGATACTCTACTCTTTCACGGAATGCGAGTCGGAGAGGTCAAAATCCGGCGTCGACGTCGTCGTCTCTGTTTCGGAGCGTGAGCCGGAGGAGCAATGAATCGATGCCGTCACGAGTGAGCAGCGGGAGAAGGAGTATCGATTCGATTTCGTCTTCTCCACTGTTGTCGAAAAGTGGTAGCCGGAGGAGCACGACTCCGATCATGTTCTCGAATTCGTCCGGGGTGCTTAAGGCGGCGCCGATCGAGAAGCAGCTTGAATGCACACTGGAGGAGCTCTGCTTTGGGTGCATGAAGAAAATTAAGGTCACAAGAGATCTCCTCTTGATCTCCGGGTTTGTCCTCTTCTTCTTTTATAAAACCCTACACCCATTCTTTTCTTTTTTTTCTTTTTCTTTATTGCTTATTATCATTTCTTTTATTTAGTATTTAATATATAAAATTAGAGATATATATAGATATAAATAATATAACATCTCTACATGACATTCACCCAGCAACCTACAAAAAGTGATTTGGTTTAGACATTGCATTTCATTTTTTAATTTCATGTTTACAGTTTCTTTCCGATGGAATGTATACATCGGACAAAATTGAGACTATTTATAAAATATAATAAAAAAAAACTTAAATGGATCAAATAGAGTTAATGGATTTTGCTATATAAATAGTTACATTATATTTTAAAATGCTTCTTCTTTTTATTATTAAAATACTATTTTAGTCCATATATTCTATATTTTCTCTATATTTTAAAATTTTACCCTATATATTCTATATTCTAAATAAATTTAAATTTAGTCATCAATGCATATTTTGTCTGAAAACAAATTTCTTATCTATAAATTTTGAAATGTATTGACATATTAAATTAATTAGACAAACAAAATTTAAGATTTATTGAAACTAGGAAATTTTTTTTAATATTTTGAAAACTAAAATTGCACTTTTAACTATTTTGTTTATTATAAGAATTTTTTTTAGTGTGAATTTTGGAAGGATAAAATAAAAAACTATATTATTTAAGAATTATATGGATGATTCATCTCTAAAGGTAATGAACTTATTATTTTAAATAATAAAACTATATTATTTCGATGTTTTGGAAATATTGTTGAAATATTATGAAAGTAAAAATAATAGAAATTAATGGATAGGCAAGCGATGGAGGAAGAAGAAACATTGACAATAAAAGTAAAGCCAGGGTGGAGAAAGGGGACAAAGATAACATTTGAAGGAGGAATGGGGAATGAGAGGGCAGGGAGTTATCCAGCGGACATGTCGTTTGTGATAGCTGAAAAACGACACTCATATTTCAAAAGAGAAGGTGACGACTTGGAGTTAACAGTGGAGATCCCTCTATTGAAAGCACTAACTGGTTGCACCATTTCAGTACCATTGTTGGGAGGAGAAACAATGAGCTTGGAGATACACGACGTGGTTTCCCCTGGATACGAGAAGCTCATTCAAGGTCAAGGTATGCCTAAACTTAAAGATGCTAACAACAGAGGCAACCTGATTGTTAAGTTTTTGGTTGACTTTCCAACTCAATTAACTCTCCAACAACGAATTGATGTTTGTCGAATTTTACAGGGTTCTCACTATTCTAGTTAAAACCAATTTGGCTTTATTATTATTAGGATTTCACTCTATACTTTTTTTTTGTTTTTTTGTTCTTTTTTTTTTGTTTTCTTTCTTTCCTTCTTTTGTTTGATTGATTGTAATCTGTATTTTGGCTTTTGGCTTTTAAGTGAAAAAAAAATGCATTCAACTTTAACTCACTAGGGCTAACTCTCTCTGGCTTGTTGGGCTTTTTAGTCCTCATACTACTTTTATCATCTTTTAGAGTTTGATTATTTACTCATAATCCCCAAATTATAAATGTTTGTTAAGTTTTATGGTTTATGGAAAATTTTCAAATATATATTTATGAAGTAAAACTCAATCTTTGTTATATTGA

mRNA sequence

CTCCCATTCATGGTGGATCTTCCTCAAGCTCTGAGCATCTCCTTTCGAAATTTCTGCAAATCCTACAAATCCTTCTTCAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGGATACCGATAATGTCGTTGTTGATACTAAACACGACGACGCTACCTCCGACGACATCCATAATAATGGACCTTCCAAGGATCATCATGAAGAAGTTAAGAAAAAGGAAGGACCGAGACCGACGACGACAAAAGGCGTTCATAGCTTTAGATATGGAGGTCGGAGTCTGAGAGAGAATGATACGACGAGTTTCAGGCCTCGAAGCTATGACAGTGGATACTCTACTCTTTCACGGAATGCGAGTCGGAGAGGTCAAAATCCGGCGTCGACGTCGTCGTCTCTGTTTCGGAGCGTGAGCCGGAGGAGCAATGAATCGATGCCGTCACGAGTGAGCAGCGGGAGAAGGAGTATCGATTCGATTTCGTCTTCTCCACTGTTGTCGAAAAGTGGTAGCCGGAGGAGCACGACTCCGATCATGTTCTCGAATTCGTCCGGGGTGCTTAAGGCGGCGCCGATCGAGAAGCAGCTTGAATGCACACTGGAGGAGCTCTGCTTTGGGTGCATGAAGAAAATTAAGGTCACAAGAGATCTCCTCTTGATCTCCGGGCAAGCGATGGAGGAAGAAGAAACATTGACAATAAAAGTAAAGCCAGGGTGGAGAAAGGGGACAAAGATAACATTTGAAGGAGGAATGGGGAATGAGAGGGCAGGGAGTTATCCAGCGGACATGTCGTTTGTGATAGCTGAAAAACGACACTCATATTTCAAAAGAGAAGGTGACGACTTGGAGTTAACAGTGGAGATCCCTCTATTGAAAGCACTAACTGGTTGCACCATTTCAGTACCATTGTTGGGAGGAGAAACAATGAGCTTGGAGATACACGACGTGGTTTCCCCTGGATACGAGAAGCTCATTCAAGGTCAAGGTATGCCTAAACTTAAAGATGCTAACAACAGAGGCAACCTGATTGTTAAGTTTTTGGTTGACTTTCCAACTCAATTAACTCTCCAACAACGAATTGATGTTTGTCGAATTTTACAGGGTTCTCACTATTCTAGTTAAAACCAATTTGGCTTTATTATTATTAGGATTTCACTCTATACTTTTTTTTTGTTTTTTTGTTCTTTTTTTTTTGTTTTCTTTCTTTCCTTCTTTTGTTTGATTGATTGTAATCTGTATTTTGGCTTTTGGCTTTTAAGTGAAAAAAAAATGCATTCAACTTTAACTCACTAGGGCTAACTCTCTCTGGCTTGTTGGGCTTTTTAGTCCTCATACTACTTTTATCATCTTTTAGAGTTTGATTATTTACTCATAATCCCCAAATTATAAATGTTTGTTAAGTTTTATGGTTTATGGAAAATTTTCAAATATATATTTATGAAGTAAAACTCAATCTTTGTTATATTGA

Coding sequence (CDS)

ATGGTGGATCTTCCTCAAGCTCTGAGCATCTCCTTTCGAAATTTCTGCAAATCCTACAAATCCTTCTTCAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGGATACCGATAATGTCGTTGTTGATACTAAACACGACGACGCTACCTCCGACGACATCCATAATAATGGACCTTCCAAGGATCATCATGAAGAAGTTAAGAAAAAGGAAGGACCGAGACCGACGACGACAAAAGGCGTTCATAGCTTTAGATATGGAGGTCGGAGTCTGAGAGAGAATGATACGACGAGTTTCAGGCCTCGAAGCTATGACAGTGGATACTCTACTCTTTCACGGAATGCGAGTCGGAGAGGTCAAAATCCGGCGTCGACGTCGTCGTCTCTGTTTCGGAGCGTGAGCCGGAGGAGCAATGAATCGATGCCGTCACGAGTGAGCAGCGGGAGAAGGAGTATCGATTCGATTTCGTCTTCTCCACTGTTGTCGAAAAGTGGTAGCCGGAGGAGCACGACTCCGATCATGTTCTCGAATTCGTCCGGGGTGCTTAAGGCGGCGCCGATCGAGAAGCAGCTTGAATGCACACTGGAGGAGCTCTGCTTTGGGTGCATGAAGAAAATTAAGGTCACAAGAGATCTCCTCTTGATCTCCGGGCAAGCGATGGAGGAAGAAGAAACATTGACAATAAAAGTAAAGCCAGGGTGGAGAAAGGGGACAAAGATAACATTTGAAGGAGGAATGGGGAATGAGAGGGCAGGGAGTTATCCAGCGGACATGTCGTTTGTGATAGCTGAAAAACGACACTCATATTTCAAAAGAGAAGGTGACGACTTGGAGTTAACAGTGGAGATCCCTCTATTGAAAGCACTAACTGGTTGCACCATTTCAGTACCATTGTTGGGAGGAGAAACAATGAGCTTGGAGATACACGACGTGGTTTCCCCTGGATACGAGAAGCTCATTCAAGGTCAAGGTATGCCTAAACTTAAAGATGCTAACAACAGAGGCAACCTGATTGTTAAGTTTTTGGTTGACTTTCCAACTCAATTAACTCTCCAACAACGAATTGATGTTTGTCGAATTTTACAGGGTTCTCACTATTCTAGTTAA

Protein sequence

MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPSKDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRILQGSHYSS
Homology
BLAST of PI0011215 vs. ExPASy Swiss-Prot
Match: P59910 (DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 6.5e-34
Identity = 77/177 (43.50%), Postives = 114/177 (64.41%), Query Frame = 0

Query: 180 GVLKAAP-IEKQLECTLEELCFGCMKKIKVTRDLLLISGQAME-EEETLTIKVKPGWRKG 239
           GV K  P +E+ L  +LE+L FGC KKIK++R +L   G +   +++ LTI VKPGWR+G
Sbjct: 132 GVKKQDPQVERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQG 191

Query: 240 TKITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVP 299
           T+ITFE   G++     PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ V 
Sbjct: 192 TRITFE-KEGDQGPNIIPADIIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVR 251

Query: 300 LLGGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQR 355
            L    +++ I+D++ P Y K + G+GMP  +D   +G+L + F + FPT+LT Q++
Sbjct: 252 TLDDRLLNIPINDIIHPKYFKKVPGEGMPLPEDPTKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of PI0011215 vs. ExPASy Swiss-Prot
Match: Q80Y75 (DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.2e-33
Identity = 73/170 (42.94%), Postives = 112/170 (65.88%), Query Frame = 0

Query: 186 PIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAME-EEETLTIKVKPGWRKGTKITFEG 245
           PIE+ L  +LE+L FGC KKIK++R +L     +   +++ LTI V+PGWR+GT+ITFE 
Sbjct: 139 PIERDLYLSLEDLFFGCTKKIKISRRVLNEDRYSSTIKDKILTIDVRPGWRQGTRITFE- 198

Query: 246 GMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETM 305
             G++     PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ V  L    +
Sbjct: 199 KEGDQGPNIIPADIIFIVKEKLHPRFRREHDNLFFVYPIPLGKALTCCTVEVKTLDDRLL 258

Query: 306 SLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQR 355
           ++ I+D+V P Y K++ G+GMP  ++ + +G+L + F + FPT+LT Q++
Sbjct: 259 NIPINDIVHPKYFKIVPGEGMPLPENPSKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of PI0011215 vs. ExPASy Swiss-Prot
Match: Q2KIT4 (DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-31
Identity = 69/166 (41.57%), Postives = 110/166 (66.27%), Query Frame = 0

Query: 186 PIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAME-EEETLTIKVKPGWRKGTKITFEG 245
           P+  +L  +LEE+  GC K++K++R  L   G++   E++ LTI++K GW++GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNPDGRSYRTEDKILTIEIKKGWKEGTKITFP- 218

Query: 246 GMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETM 305
             G+E   S PAD+ FVI +K H  FKR+G ++  T +I L +AL GC+I+VP + G T+
Sbjct: 219 REGDETPTSIPADIVFVIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTMDGRTI 278

Query: 306 SLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLT 351
            + I+D+V PG  + I G G+P  K+ + RG+L+++F V FP  ++
Sbjct: 279 PMTINDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTIS 323

BLAST of PI0011215 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-31
Identity = 67/166 (40.36%), Postives = 109/166 (65.66%), Query Frame = 0

Query: 186 PIEKQLECTLEELCFGCMKKIKVTRDLLLISGQA-MEEEETLTIKVKPGWRKGTKITFEG 245
           P+  +L  +LEE+  GC K++K++R  L   G++   E++ LTI++K GW++GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFP- 218

Query: 246 GMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETM 305
             G+E   S PAD+ F+I +K H  FKR+G ++  T +I L +AL GC+I+VP L G  +
Sbjct: 219 REGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNI 278

Query: 306 SLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLT 351
            + ++D+V PG  + I G G+P  K+ + RG+L+++F V FP  ++
Sbjct: 279 PMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTIS 323

BLAST of PI0011215 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-31
Identity = 67/166 (40.36%), Postives = 109/166 (65.66%), Query Frame = 0

Query: 186 PIEKQLECTLEELCFGCMKKIKVTRDLLLISGQA-MEEEETLTIKVKPGWRKGTKITFEG 245
           P+  +L  +LEE+  GC K++K++R  L   G++   E++ LTI++K GW++GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFP- 218

Query: 246 GMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETM 305
             G+E   S PAD+ F+I +K H  FKR+G ++  T +I L +AL GC+I+VP L G  +
Sbjct: 219 REGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNI 278

Query: 306 SLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLT 351
            + ++D+V PG  + I G G+P  K+ + RG+L+++F V FP  ++
Sbjct: 279 PMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTIS 323

BLAST of PI0011215 vs. ExPASy TrEMBL
Match: A0A1S3BAK8 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.2e-185
Identity = 345/369 (93.50%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSKVKNSKKDTDN VVDTKHDDATSD IHN+ PS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDN-VVDTKHDDATSDAIHNHSPS 60

Query: 61  KDHHEEVKKKEGPRP-TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
           KD HEE+KKKEGPRP TTT+GVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG
Sbjct: 61  KD-HEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
           QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLI+GQAMEEEETLTIKVKPGWRKGTK
Sbjct: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFEGG GNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTI VPLL
Sbjct: 241 ITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GGETMSLEIH+VVSPGYEKLIQGQGMPKLKD NNRGNLIVKF V FPTQLT QQR DVCR
Sbjct: 301 GGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVCR 360

Query: 361 ILQGSHYSS 369
           IL+ SHY S
Sbjct: 361 ILEASHYPS 367

BLAST of PI0011215 vs. ExPASy TrEMBL
Match: A0A0A0KYJ4 (DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.8e-180
Identity = 334/367 (91.01%), Postives = 349/367 (95.10%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDD TSD IHN GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQ 120
           KD HE++KKKEGPRP  TKGVHSFRYGGRSLRENDTTSFRP+SYDSGYSTLSRNASRRGQ
Sbjct: 61  KD-HEDIKKKEGPRP--TKGVHSFRYGGRSLRENDTTSFRPQSYDSGYSTLSRNASRRGQ 120

Query: 121 NPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSG 180
           N  STSSSLFRS+SRRSNESM SRVSSGRRSIDSISSSPLLSKSGS+RSTTPIMFSNSSG
Sbjct: 121 NAGSTSSSLFRSMSRRSNESMTSRVSSGRRSIDSISSSPLLSKSGSKRSTTPIMFSNSSG 180

Query: 181 VLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKI 240
           VLKAA IEKQLECTLEELCFGC+KKIKVTRDLLLI+GQAMEEEETLT+KVKPGWRKGTKI
Sbjct: 181 VLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLLINGQAMEEEETLTMKVKPGWRKGTKI 240

Query: 241 TFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLG 300
           TFEGGMGNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTISVPLLG
Sbjct: 241 TFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISVPLLG 300

Query: 301 GETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRI 360
           GETMSL+IH+VVSPGYEKLIQGQGMPKLKD + RG+LI+KF VDFPTQLT QQR DVCRI
Sbjct: 301 GETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSDVCRI 360

Query: 361 LQGSHYS 368
           L+GSH+S
Sbjct: 361 LEGSHHS 364

BLAST of PI0011215 vs. ExPASy TrEMBL
Match: A0A5D3E349 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold587G00120 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.5e-166
Identity = 316/369 (85.64%), Postives = 322/369 (87.26%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSK                               
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSK------------------------------- 60

Query: 61  KDHHEEVKKKEGPRP-TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
              HEE+KKKEGPRP TTT+GVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG
Sbjct: 61  --DHEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
           QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLI+GQAMEEEETLTIKVKPGWRKGTK
Sbjct: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFEGG GNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTI VPLL
Sbjct: 241 ITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GGETMSLEIH+VVSPGYEKLIQGQGMPKLKD NNRGNLIVKF V FPTQLT QQR DVCR
Sbjct: 301 GGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVCR 336

Query: 361 ILQGSHYSS 369
           IL+ SHY S
Sbjct: 361 ILEASHYPS 336

BLAST of PI0011215 vs. ExPASy TrEMBL
Match: A0A6J1FLF5 (dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111446483 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.5e-139
Identity = 275/368 (74.73%), Postives = 310/368 (84.24%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDAT-SDDIHNNGP 60
           MVDLPQ L +SFRNFCKSYKSFFKKWHSKVKNSKK+TD  VVD+KHD ++ + D +NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTD--VVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
           SKD  E    KEGPR  T KG  SFRY  RS+REND    R RSYD   S++ RNASRRG
Sbjct: 61  SKDREE--INKEGPRQMTKKGGLSFRYASRSMRENDPKISRHRSYD---SSMFRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
            NP  +SSS FRSVSRRS ESMPSR++SG  S+DS SSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 LNPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLK  PIEKQLECTLEELCFGCMKKIKVTRD++L +GQA+EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFE GMGNER GSYPAD+SFVI EK+H YFKREGDDL+LTVEIPLLKALTGCTIS+PLL
Sbjct: 241 ITFE-GMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GG+ MSL  HDV+  G  K+I+GQGMPKLK+  NRGNL VKFL++FPT+LT QQR DVCR
Sbjct: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 359

Query: 361 ILQGSHYS 368
           IL+GS+YS
Sbjct: 361 ILEGSNYS 359

BLAST of PI0011215 vs. ExPASy TrEMBL
Match: A0A6J1ISU0 (dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480292 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.3e-138
Identity = 274/368 (74.46%), Postives = 311/368 (84.51%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDAT-SDDIHNNGP 60
           MVDLPQ L +SFRNFCKSYKSFFKKWHSKVKNSKK+TD  VVD+KHD ++ + D +NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTD--VVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
           SKD  E    KEGPR  T KG  SFRYG RS+REND    R RSYD   S++ RNASRRG
Sbjct: 61  SKDREE--ISKEGPRQMTKKGGLSFRYGSRSMRENDPKISRHRSYD---SSMFRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
            NP  +SSS FRSVSRRS ESMPSR++SG  S+DS SSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 LNPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLK APIEKQLECTLEELCFGCMKKIKVTRD++L +GQA+EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPAPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFE GMGNER GSYPAD+SFVI EK+H YFKREGDDL+LTVEIPL+KALTGCTIS+PLL
Sbjct: 241 ITFE-GMGNERLGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLVKALTGCTISIPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GG+ MSL+  DV+  G  K+I+GQGMPKLK+  NRGNL VKFL +FPT+LT QQR D+CR
Sbjct: 301 GGDIMSLKTQDVIWHGCVKIIKGQGMPKLKEEINRGNLKVKFLTEFPTELTDQQRSDICR 359

Query: 361 ILQGSHYS 368
           IL+GS+YS
Sbjct: 361 ILEGSNYS 359

BLAST of PI0011215 vs. NCBI nr
Match: XP_008444178.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 657.1 bits (1694), Expect = 8.7e-185
Identity = 345/369 (93.50%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSKVKNSKKDTDN VVDTKHDDATSD IHN+ PS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDN-VVDTKHDDATSDAIHNHSPS 60

Query: 61  KDHHEEVKKKEGPRP-TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
           KD HEE+KKKEGPRP TTT+GVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG
Sbjct: 61  KD-HEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
           QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLI+GQAMEEEETLTIKVKPGWRKGTK
Sbjct: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFEGG GNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTI VPLL
Sbjct: 241 ITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GGETMSLEIH+VVSPGYEKLIQGQGMPKLKD NNRGNLIVKF V FPTQLT QQR DVCR
Sbjct: 301 GGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVCR 360

Query: 361 ILQGSHYSS 369
           IL+ SHY S
Sbjct: 361 ILEASHYPS 367

BLAST of PI0011215 vs. NCBI nr
Match: XP_004142703.1 (uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical protein Csa_012239 [Cucumis sativus])

HSP 1 Score: 641.7 bits (1654), Expect = 3.8e-180
Identity = 334/367 (91.01%), Postives = 349/367 (95.10%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDD TSD IHN GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQ 120
           KD HE++KKKEGPRP  TKGVHSFRYGGRSLRENDTTSFRP+SYDSGYSTLSRNASRRGQ
Sbjct: 61  KD-HEDIKKKEGPRP--TKGVHSFRYGGRSLRENDTTSFRPQSYDSGYSTLSRNASRRGQ 120

Query: 121 NPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSG 180
           N  STSSSLFRS+SRRSNESM SRVSSGRRSIDSISSSPLLSKSGS+RSTTPIMFSNSSG
Sbjct: 121 NAGSTSSSLFRSMSRRSNESMTSRVSSGRRSIDSISSSPLLSKSGSKRSTTPIMFSNSSG 180

Query: 181 VLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKI 240
           VLKAA IEKQLECTLEELCFGC+KKIKVTRDLLLI+GQAMEEEETLT+KVKPGWRKGTKI
Sbjct: 181 VLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLLINGQAMEEEETLTMKVKPGWRKGTKI 240

Query: 241 TFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLG 300
           TFEGGMGNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTISVPLLG
Sbjct: 241 TFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISVPLLG 300

Query: 301 GETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRI 360
           GETMSL+IH+VVSPGYEKLIQGQGMPKLKD + RG+LI+KF VDFPTQLT QQR DVCRI
Sbjct: 301 GETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSDVCRI 360

Query: 361 LQGSHYS 368
           L+GSH+S
Sbjct: 361 LEGSHHS 364

BLAST of PI0011215 vs. NCBI nr
Match: TYK30011.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 595.5 bits (1534), Expect = 3.1e-166
Identity = 316/369 (85.64%), Postives = 322/369 (87.26%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSK                               
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSK------------------------------- 60

Query: 61  KDHHEEVKKKEGPRP-TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
              HEE+KKKEGPRP TTT+GVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG
Sbjct: 61  --DHEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
           QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLI+GQAMEEEETLTIKVKPGWRKGTK
Sbjct: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFEGG GNERAGSYPAD SFVIAEKRHSYFKREGDDLEL VEIPLLKALTGCTI VPLL
Sbjct: 241 ITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GGETMSLEIH+VVSPGYEKLIQGQGMPKLKD NNRGNLIVKF V FPTQLT QQR DVCR
Sbjct: 301 GGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVCR 336

Query: 361 ILQGSHYSS 369
           IL+ SHY S
Sbjct: 361 ILEASHYPS 336

BLAST of PI0011215 vs. NCBI nr
Match: XP_038899191.1 (dnaJ homolog subfamily B member 4-like [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 7.2e-163
Identity = 315/367 (85.83%), Postives = 331/367 (90.19%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPS 60
           MVDLPQAL ISFRNFCKSYKSFFKKWHSK KNSKKD D  VVDTKH DATSD I NNGPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKAKNSKKDAD--VVDTKH-DATSDAIGNNGPS 60

Query: 61  KDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQ 120
           KD HEEVKK EG  PTT K VHS RYGG+S+REND TSFRPRSYDSG S+LSRNASRRGQ
Sbjct: 61  KD-HEEVKKTEG--PTTMKSVHSLRYGGQSMRENDATSFRPRSYDSGVSSLSRNASRRGQ 120

Query: 121 NPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSG 180
           NP+STSSSLFRSVSRRS ES+PSRVSSGRRSIDS+SSSPLLSKSGSR STTPIMFSNSSG
Sbjct: 121 NPSSTSSSLFRSVSRRSTESVPSRVSSGRRSIDSVSSSPLLSKSGSRMSTTPIMFSNSSG 180

Query: 181 VLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKI 240
           +LK APIEKQLECTLEELCFGCMKKIKVTRDL+LISG+ MEEEETLTIK+KPGW KGTKI
Sbjct: 181 LLKPAPIEKQLECTLEELCFGCMKKIKVTRDLILISGKTMEEEETLTIKIKPGWTKGTKI 240

Query: 241 TFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLG 300
           TFE GMGNER GSYPADMSFVI EKRH YFKREGDDLELTVEIPLLKAL GC ISVPLLG
Sbjct: 241 TFE-GMGNERPGSYPADMSFVIVEKRHPYFKREGDDLELTVEIPLLKALIGCNISVPLLG 300

Query: 301 GETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRI 360
           GETMSL+IHDVV PGYEK+IQGQGMPKLKD + RGNL VKFLV+FPT+LT QQR DVCRI
Sbjct: 301 GETMSLKIHDVVWPGYEKVIQGQGMPKLKD-DKRGNLKVKFLVEFPTELTDQQRYDVCRI 359

Query: 361 LQGSHYS 368
           L+ S YS
Sbjct: 361 LEDSDYS 359

BLAST of PI0011215 vs. NCBI nr
Match: XP_022941087.1 (dnaJ homolog subfamily B member 5-like [Cucurbita moschata])

HSP 1 Score: 504.6 bits (1298), Expect = 7.2e-139
Identity = 275/368 (74.73%), Postives = 310/368 (84.24%), Query Frame = 0

Query: 1   MVDLPQALSISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDAT-SDDIHNNGP 60
           MVDLPQ L +SFRNFCKSYKSFFKKWHSKVKNSKK+TD  VVD+KHD ++ + D +NNGP
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTD--VVDSKHDGSSDAIDNNNNGP 60

Query: 61  SKDHHEEVKKKEGPRPTTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120
           SKD  E    KEGPR  T KG  SFRY  RS+REND    R RSYD   S++ RNASRRG
Sbjct: 61  SKDREE--INKEGPRQMTKKGGLSFRYASRSMRENDPKISRHRSYD---SSMFRNASRRG 120

Query: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSS 180
            NP  +SSS FRSVSRRS ESMPSR++SG  S+DS SSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 LNPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTK 240
           GVLK  PIEKQLECTLEELCFGCMKKIKVTRD++L +GQA+EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240

Query: 241 ITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLL 300
           ITFE GMGNER GSYPAD+SFVI EK+H YFKREGDDL+LTVEIPLLKALTGCTIS+PLL
Sbjct: 241 ITFE-GMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300

Query: 301 GGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCR 360
           GG+ MSL  HDV+  G  K+I+GQGMPKLK+  NRGNL VKFL++FPT+LT QQR DVCR
Sbjct: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 359

Query: 361 ILQGSHYS 368
           IL+GS+YS
Sbjct: 361 ILEGSNYS 359

BLAST of PI0011215 vs. TAIR 10
Match: AT1G11040.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-60
Identity = 127/257 (49.42%), Postives = 178/257 (69.26%), Query Frame = 0

Query: 106 SGYSTLSRNASRRGQNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSG 165
           S  S++S++AS+R ++   ++SS        ++ S+P   S   R  D+  S   ++KS 
Sbjct: 190 SSPSSISKSASKRDKDERGSTSS-------ATSTSLPYSKSKSTR--DTAGS---IAKSI 249

Query: 166 SRRSTTPIMFSNSSGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLISGQAMEEEET 225
           SRRSTTPI+FS S+   K   +EK+LECTLEELC G +K IK+ RD++   G  M++EE 
Sbjct: 250 SRRSTTPIVFSQSTPPKKPPAVEKKLECTLEELCHGGVKNIKIKRDIITDEGLIMQQEEM 309

Query: 226 LTIKVKPGWRKGTKITFEGGMGNERAGSYPADMSFVIAEKRHSYFKREGDDLELTVEIPL 285
           L + ++PGW+KGTKITFE G+GNE+ G  P D++FV+ EKRH  FKR GDDLE+ VEIPL
Sbjct: 310 LRVNIQPGWKKGTKITFE-GVGNEKPGYLPEDITFVVEEKRHPLFKRRGDDLEIAVEIPL 369

Query: 286 LKALTGCTISVPLLGGETMSLEIHDVVSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDF 345
           LKALTGC +SVPLL GE+MS+ + DV+  G+EK I+GQGMP  K+   RG+L + FLV+F
Sbjct: 370 LKALTGCKLSVPLLSGESMSITVGDVIFHGFEKAIKGQGMPNAKEEGKRGDLRITFLVNF 429

Query: 346 PTQLTLQQRIDVCRILQ 363
           P +L+ +QR     +L+
Sbjct: 430 PEKLSEEQRSMAYEVLK 433

BLAST of PI0011215 vs. TAIR 10
Match: AT1G44160.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.1e-56
Identity = 147/347 (42.36%), Postives = 195/347 (56.20%), Query Frame = 0

Query: 25  KWHSKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPSKDHHEEVK---KKEGPRPTTTKGV 84
           K H K K+  K+T    +D +H          N P+     E K   K       +   V
Sbjct: 38  KHHHKAKHDTKETKR--LDEEH--------KRNTPNAFEFRETKGIGKNNKVEGVSLCKV 97

Query: 85  HSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQNPASTSSSLFRSVSRRSNESM 144
            S+R+      +N  T+F          +LSR+ S+      ST+ +L      RS   +
Sbjct: 98  RSYRF------DNTNTNF-----VGSKKSLSRSCSQNTATATSTNPTL------RSLSFI 157

Query: 145 PSRVSSGRRSIDSISSSPLLSKSGS---RRSTTPIMFSNSSG-VLKAAPIEKQLECTLEE 204
               SS  R  +S    P L +S +   R    PI++S+SS  V K +P EK+L CTLEE
Sbjct: 158 GRSKSSSNRMTESGGFMPTLMRSTTTVPRSFANPILYSSSSAKVAKPSPTEKKLRCTLEE 217

Query: 205 LCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKITFEGGMGNERAGSYPAD 264
           LC GC KKIK+ RD++   G+  EEEE + IKVKPGW+ GTK+TFE G GNE   S PAD
Sbjct: 218 LCNGCTKKIKIKRDVITSLGEKCEEEEMVEIKVKPGWKGGTKVTFE-GKGNEAMRSVPAD 277

Query: 265 MSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETMSLEIHDVVSPGYE 324
           ++FVI EK H  FKREGDDLE+ VE+ LL+ALTGC +SV LL G+ M L I DV+ PGY 
Sbjct: 278 LTFVIVEKEHEVFKREGDDLEMAVEVSLLEALTGCELSVALLDGDNMRLRIEDVIHPGYV 337

Query: 325 KLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRILQGS 365
            ++QG+GMP LK+   RG+L V+F   FP  LT +QR ++  ILQ S
Sbjct: 338 TVVQGKGMPNLKEKGKRGDLRVRFRTKFPQHLTDEQRAEIHSILQDS 356

BLAST of PI0011215 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.4e-44
Identity = 124/349 (35.53%), Postives = 183/349 (52.44%), Query Frame = 0

Query: 17  KSYKSFFKKWH-SKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPSKDHHEEVKKKEGPRP 76
           K+Y+    KWH  K  N+KKD +              D         + EE    + P P
Sbjct: 22  KAYRKLAMKWHPDKNPNNKKDAEAKFKQISEAYDVLSDPQKRAIYDQYGEEGLTSQAPPP 81

Query: 77  TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQNPASTSSSLFRSVSR 136
               G   F  GG S R      F  RS D  +S                    F   +R
Sbjct: 82  GAGGG---FSDGGASFR------FNGRSADDIFSE-------------------FFGFTR 141

Query: 137 RSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSGVLKAAPIEKQLECTL 196
              +S  +  S+G R  + + SS ++                     KAAPIE+QL C+L
Sbjct: 142 PFGDSRGAGPSNGFRFAEDVFSSNVVPPR------------------KAAPIERQLPCSL 201

Query: 197 EELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKITFEGGMGNERAGSYP 256
           E+L  G  KK+K++RD+L  SG+    EE LTI++KPGW+KGTKITF    GNE+ G  P
Sbjct: 202 EDLYKGVSKKMKISRDVLDSSGRPTTVEEILTIEIKPGWKKGTKITFP-EKGNEQRGIIP 261

Query: 257 ADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETMSLEIHDVVSPG 316
           +D+ F++ EK H+ FKR+G+DL +T +IPL++ALTG T  V  L G ++++ I++V+SP 
Sbjct: 262 SDLVFIVDEKPHAVFKRDGNDLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPS 321

Query: 317 YEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRILQGS 365
           YE++++G+GMP  KD + +GNL +KF V FP++LT +Q+  + R+   S
Sbjct: 322 YEEVVKGEGMPIPKDPSKKGNLRIKFTVKFPSRLTTEQKSGIKRMFSSS 323

BLAST of PI0011215 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-40
Identity = 125/350 (35.71%), Postives = 181/350 (51.71%), Query Frame = 0

Query: 17  KSYKSFFKKWH-SKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPSKDHHEEVKKKEGPRP 76
           K+Y+    KWH  K  N+KKD + +            D         + EE  K   P P
Sbjct: 22  KAYRKLAMKWHPDKNPNNKKDAEAMFKQISEAYEVLSDPQKKAVYDQYGEEGLKGNVP-P 81

Query: 77  TTTKGVHSFRYGGRSLRENDTTSFR--PRSYDSGYSTLSRNASRRGQNPAST--SSSLFR 136
               G   F  G      +  TSFR  PR+ D  ++     +S  G     T  SSS+F 
Sbjct: 82  PDAGGATYFSTG------DGPTSFRFNPRNADDIFAEFFGFSSPFGGGRGGTRFSSSMFG 141

Query: 137 SVSRRSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSGVLKAAPIEKQL 196
                                D++ +S      G   S       +  G  KAAPIE +L
Sbjct: 142 ---------------------DNMFASFGEGGGGGGGS------MHHGGARKAAPIENKL 201

Query: 197 ECTLEELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKITFEGGMGNERA 256
            C+LE+L  G  KK++++R++  +SG+ M+ EE LTI VKPGW+KGTKITF    GNE+ 
Sbjct: 202 PCSLEDLYKGTTKKMRISREIADVSGKTMQVEEILTIDVKPGWKKGTKITFP-EKGNEQP 261

Query: 257 GSYPADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETMSLEIHDV 316
           G  PAD+ F+I EK H  F REG+DL +T +I L++ALTG T+++  L G  +++ + +V
Sbjct: 262 GVIPADLVFIIDEKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTLDGRRLTIPVTNV 321

Query: 317 VSPGYEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRIL 362
           V P YE+++  +GMP  KD   RGNL +KF + FPT+LT +Q+  V ++L
Sbjct: 322 VHPEYEEVVPKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKLL 336

BLAST of PI0011215 vs. TAIR 10
Match: AT5G01390.1 (DNAJ heat shock family protein )

HSP 1 Score: 162.5 bits (410), Expect = 6.3e-40
Identity = 120/346 (34.68%), Postives = 178/346 (51.45%), Query Frame = 0

Query: 17  KSYKSFFKKWH-SKVKNSKKDTDNVVVDTKHDDATSDDIHNNGPSKDHHEEVKKKEGPRP 76
           K+Y+    KWH  K  N+KK+ +              D       + + EE    + P P
Sbjct: 22  KAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDPQKRAIYEQYGEE-GLNQAPPP 81

Query: 77  TTTKGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRGQNPASTSSSLFRSVSR 136
               G       G S R      F PRS D  +S               T  S       
Sbjct: 82  GAGGGYPGGSDAGASFR------FNPRSADDIFSEFF----------GFTRPSFGTGSDS 141

Query: 137 RSNESMPSRVSSGRRSIDSISSSPLLSKSGSRRSTTPIMFSNSSGVLKAAPIEKQLECTL 196
           R+         SG R  D I +S   + +G   S             K+APIE+QL C+L
Sbjct: 142 RAG-------PSGFRYGDDIFASFRAATTGGEASIPS---------RKSAPIERQLPCSL 201

Query: 197 EELCFGCMKKIKVTRDLLLISGQAMEEEETLTIKVKPGWRKGTKITFEGGMGNERAGSYP 256
           E+L  G  KK+K++RD+L  SG+    EE LTI++KPGW+KGTKITF    GNE  G  P
Sbjct: 202 EDLYKGVSKKMKISRDVLDSSGRPTPVEEILTIEIKPGWKKGTKITFL-EKGNEHRGVIP 261

Query: 257 ADMSFVIAEKRHSYFKREGDDLELTVEIPLLKALTGCTISVPLLGGETMSLEIHDVVSPG 316
           +D+ F++ EK H  FKR+G+DL +  +I L+ ALTG T  V  L G T+++ +++V+SP 
Sbjct: 262 SDLVFIVDEKPHPVFKRDGNDLVVMQKISLVDALTGYTAQVTTLDGRTLTVPVNNVISPS 321

Query: 317 YEKLIQGQGMPKLKDANNRGNLIVKFLVDFPTQLTLQQRIDVCRIL 362
           YE++++G+GMP  KD + +GNL ++F++ FP++LT +Q+  + R+L
Sbjct: 322 YEEVVKGEGMPIPKDPSRKGNLRIRFIIKFPSKLTTEQKSGIKRML 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P599106.5e-3443.50DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1[more]
Q80Y754.2e-3342.94DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1[more]
Q2KIT41.0e-3141.57DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1[more]
Q9UDY41.0e-3140.36DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1[more]
Q5R8J81.0e-3140.36DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BAK84.2e-18593.50chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1[more]
A0A0A0KYJ41.8e-18091.01DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4... [more]
A0A5D3E3491.5e-16685.64Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5... [more]
A0A6J1FLF53.5e-13974.73dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1ISU01.3e-13874.46dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114802... [more]
Match NameE-valueIdentityDescription
XP_008444178.18.7e-18593.50PREDICTED: chaperone protein DnaJ [Cucumis melo][more]
XP_004142703.13.8e-18091.01uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical ... [more]
TYK30011.13.1e-16685.64chaperone protein DnaJ [Cucumis melo var. makuwa][more]
XP_038899191.17.2e-16385.83dnaJ homolog subfamily B member 4-like [Benincasa hispida][more]
XP_022941087.17.2e-13974.73dnaJ homolog subfamily B member 5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G11040.11.4e-6049.42HSP40/DnaJ peptide-binding protein [more]
AT1G44160.12.1e-5642.36HSP40/DnaJ peptide-binding protein [more]
AT3G08910.11.4e-4435.53DNAJ heat shock family protein [more]
AT2G20560.11.3e-4035.71DNAJ heat shock family protein [more]
AT5G01390.16.3e-4034.68DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 187..346
e-value: 1.0E-39
score: 136.2
NoneNo IPR availableGENE3D2.60.260.20coord: 186..266
e-value: 2.6E-25
score: 90.3
NoneNo IPR availableGENE3D2.60.260.20coord: 267..347
e-value: 7.5E-25
score: 89.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 100..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..76
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 69..362
NoneNo IPR availablePANTHERPTHR24078:SF535DNAJ HOMOLOG SUBFAMILY B MEMBER 13-LIKEcoord: 69..362
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 185..349
e-value: 1.17503E-48
score: 159.513
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 269..354
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 187..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0011215.1PI0011215.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005829 cytosol
molecular_function GO:0051087 chaperone binding
molecular_function GO:0051082 unfolded protein binding