PI0011111 (gene) Melon (PI 482460) v1

Overview


Name	PI0011111
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA ligase
Location	chr08: 5822605 .. 5825727 (-)
RNA-Seq Expression	PI0011111
Synteny	PI0011111

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGCTGCTCTCGCTGTTGCCAATAAGCGTAAAGCCCTTGATTCCTTTGACCCTTTTCCCAAACGTAACTCTTCCCTTCCTCTGGTAAAGTCTGAGGACGTTGCAGATTTGGGAGAAATCAAGTTGCGTCAGAATAGTGTTGCAAACAAGAGGTGTGCATCTAATGTTCAAGAAAGAACTTCAAAATTGAAGAGCTCAATCTTCGAATTGAAAAAGAAGGCCAAGGATTTCAACTGGAAGAATGTTGGATGTTGGAAAGAGGGTGAACGGGTTCCTTTTTTTGTTTCAGTATTTGAGATTTGATATGATTTCAAAGGAGTCTAGTAGGATTACTATCATTGATATTGTGTGCAATATGTTAAGGATCGTCATGGATACTACACCTGATGACTTGGTAGCCACCGTGTATCTTTTGGTGAATAAGATTGCTCTAGCTCATGAAGAGCTTGAGTTGGGGATTGGGGATGCATCTATTATCAAAGCTCTTTCAGTTTCTAAAGCATTTGGGAGGACAGAGACACAAGTAAAAAAATAGGACAATGTATTGTTGTAAATGGACGTCGAAAGGAATTGTTGTCCTGTGACGAACCACTACCCTGAAAGCAATTACAACACGACCGTGGTGCTAATCAAGATGTTGGTTGCTCTCCAAAGTTAACACAAATTCCTTATTTAGATGTGCACTCATTAGAATAAGGTCGTTAGAATAAGGAATAGAATCAAGATGAAGAAATTTCATAGCAAAAGCTGCAATCAAGATGGAGATTGAGAGAGGCAGATCTGAATATTAGATAATCAGATCTAATGAGGAAGAAGATAGTTAAAATTATAAATACAAAAAAGTTTAATTTCCAAATATGAGTTATAAAAAATAAACCGAAAAATAATAAAAAGTTATTTTTTTCAAGAAAAACTATGCTATCACAACAACACACTTAAACATCAAAGCTAGCATGCAGATATTTATACCCTTTTGACATTGCTGAGTTCTCCAATGTAGGACAAAATTATTGTCCTTTGCTTATGGGTCTTGTCCTTTGCTTATGGGCCTAAGCCCATTTCCAACAAAGGTAAGGAAGCTTTTGAGCTCCATCTTCCAAATTTAGGATGTTTTTTTTGGAGATGAAGATTGCAGATTTAATGTTTGGTGATTTAAGGTTACGGGGACCAGTGGCGAATCCAGAAATCCACTTTAACAGGGGCACAATTGTATATTTTTATTTATTTTATACCTAAAATTTAATAAAATTTTCCTTTTTATTGTAGCAAGACTCGATTTTTATTTTTTATTTAAGGGACTTAAATTTTTCATTTTTATTTAGAGGAAGACTCCACAAAGCCATTAAAACAAAAATTGCACAAGAAAACAAAAAAGTGGTGTCAACAAGATTTGAACCCATGACCCCATATTTCCTAGGTAATTACTTATATTTTACATTAAAATATAAATACCGATAATTTGAGGGGGGCACGTGCCCCCGGTGGTCCCTATGTAAATCCGCCGGTGTAGGGAACAATTGGGACGACAATCTGGAAGTTTTCATACTCTTGGATTTATTTTCATTATAACTTGCTGCACAGAGTTTGTAAATGAAATGATAAAAATTCCTTTTTTTTTTTTTTTTTGTTTTTTGTTTTCATTCAGTATTAAAGATTTCCAAAGGGTCTCAAGAATGTCACAATTCACCACTTTCCTGGTGTATTAGGAATTTGTCACTGAATACTATTTTAACGACAAACATTTTATATTTTAATGTCTCAATTGTTTGACAGGAATTGGGAGATTTTAGGTCTTGTTGCTAAATCGAGTCTTTCATCTCAATCTATGATGCGAAAGCCTGATGCTTTAACTATTACCAAAGTATTTGACACCTTTGCTAAGGTGTGTTAGGCTCGTGAATTGCTTAATCCATAACCAATAAACTAGGTTGCAGTTAGTTTGAATGCAAACTATTAACTAGATGGAGTGCAATTACCATGTATTAAATTCAGCTGCTTTCTAAGTTTTAAAGTTATACAGAATTTCTCATATTATCGCGTTTTTGTGATTCTGTAAATGTGAGATGTTGAATTTCCTCTATTATGCGTAGTAAATTTGTGGCCATAAACTCATATTTATTTTGAAGATTTCCTTTTCACCCTTTATGAATTGTTCTCGGGAATCTGGCAAGCATAGTGAGGAGAAGAATAAAAACCATATCAAGTCACTGTTGCTGCCACTGACTGCGAACCTTTATATCGGATTAGATTGCTGCAAGTTATGTCTCATCTTTGTTAGAATTAATAATGTCAGCCTCTTTTTGATAAATAAATAATGTTTTGTTTCTTTCTCTATTGCCAATTTTCTTTCATATTATGCAGACAAAATTGCGAATTGAACTTGCTGAGCAGACTCTTTTAGCTGCATTAGAGCAAGCTGCTGTATATGCTGAAAGCATTCAACACCACCACCATCATATTCAATCTCTTTTAGAAGAGGTGACTTCATGTACTCTGTGTCTCTGGGATCAAATTCCTTCATTTTATTACCATCTTTTTGGGCAATGTCATTGTTTTAATTATGAGACTCTAAGGTATTATCATAAAGTATCACGTTTATAACAATACCTACACAACTTGGCTTGGTTACTAGTTTACTTGCATAATGCCATTTTCTGAGTTCTATGTTCTCATATCAGTTGGAAGGCTCTAACGTTATGTTTGCATCAGGTTTCTAAGATTGTCAAGCAGGTGTATTCCATACTCTCAGTTTATGATAAACTTGTTCCTGCTCTTCTTAGTGGTGGTGTGTGGAATTTTTCAAAAACATGCAACTTTACACAGAGAATTCCAATTGGACCGCGCTGACAAAACCAACAAAAGGTGTTTCTGAGATCTTAGATAAATTCCAGGGTTTAGAATTTACTTGTGAGTACAAATATGATGAGGAATGTGCCTAGTTGAGTGAACGAATAACTTGAAAAATGAAGTTTCTCCGGCTATTTAATATTCACATGGAATGACTTCAGTCTCTTTATGCAGATTCATTACATGGAAGATGGTTTAGTTGAGATATACAGACGAAATGCTGAACGGAATACTGGAAAGTTCCCTGATGATGTTCTCACTGTATCAAGGTAG

mRNA sequence

ATGTCTGCTGCTCTCGCTGTTGCCAATAAGCGTAAAGCCCTTGATTCCTTTGACCCTTTTCCCAAACGTAACTCTTCCCTTCCTCTGGTAAAGTCTGAGGACGTTGCAGATTTGGGAGAAATCAAGTTGCGTCAGAATAGTGTTGCAAACAAGAGGTGTGCATCTAATGTTCAAGAAAGAACTTCAAAATTGAAGAGCTCAATCTTCGAATTGAAAAAGAAGGCCAAGGATTTCAACTGGAAGAATGTTGGATGTTGGAAAGAGGGTGAACGGTATTTGAGATTTGATATGATTTCAAAGGAGTCTAGTAGGATTACTATCATTGATATTGTGTGCAATATGTTAAGGATCGTCATGGATACTACACCTGATGACTTGGTAGCCACCGTGTATCTTTTGGTGAATAAGATTGCTCTAGCTCATGAAGAGCTTGAGTTGGGGATTGGGGATGCATCTATTATCAAAGCTCTTTCAGTTTCTAAAGCATTTGGGAGGACAGAGACACAAGACAAAATTATTGTCCTTTGCTTATGGGTCTTGTCCTTTGCTTATGGGCCTAAGCCCATTTCCAACAAAGGTTACGGGGACCAGTGGCGAATCCAGAAATCCACTTTAACAGGGGCACAATTAGGAAGACTCCACAAAGCCATTAAAACAAAAATTGCACAAGAAAACAAAAAAGTGGTGTCAACAAGATTTGAACCCATGACCCCATATTTCCTAGTATTAAAGATTTCCAAAGGGTCTCAAGAATGTCACAATTCACCACTTTCCTGGAATTGGGAGATTTTAGGTCTTGTTGCTAAATCGAGTCTTTCATCTCAATCTATGATGCGAAAGCCTGATGCTTTAACTATTACCAAAGTATTTGACACCTTTGCTAAGACAAAATTGCGAATTGAACTTGCTGAGCAGACTCTTTTAGCTGCATTAGAGCAAGCTGCTGTATATGCTGAAAGCATTCAACACCACCACCATCATATTCAATCTCTTTTAGAAGAGGTGACTTCATGTACTCTGTGTCTCTGGGATCAAATTCCTTCATTTTATTACCATCTTTTTGGGCAATGTCATTGTTTTAATTATGAGACTCTAAGTTGGAAGGCTCTAACGTTATGTTTGCATCAGGTTTCTAAGATTGTCAAGCAGATTCATTACATGGAAGATGGTTTAGTTGAGATATACAGACGAAATGCTGAACGGAATACTGGAAAGTTCCCTGATGATGTTCTCACTGTATCAAGGTAG

Coding sequence (CDS)

ATGTCTGCTGCTCTCGCTGTTGCCAATAAGCGTAAAGCCCTTGATTCCTTTGACCCTTTTCCCAAACGTAACTCTTCCCTTCCTCTGGTAAAGTCTGAGGACGTTGCAGATTTGGGAGAAATCAAGTTGCGTCAGAATAGTGTTGCAAACAAGAGGTGTGCATCTAATGTTCAAGAAAGAACTTCAAAATTGAAGAGCTCAATCTTCGAATTGAAAAAGAAGGCCAAGGATTTCAACTGGAAGAATGTTGGATGTTGGAAAGAGGGTGAACGGTATTTGAGATTTGATATGATTTCAAAGGAGTCTAGTAGGATTACTATCATTGATATTGTGTGCAATATGTTAAGGATCGTCATGGATACTACACCTGATGACTTGGTAGCCACCGTGTATCTTTTGGTGAATAAGATTGCTCTAGCTCATGAAGAGCTTGAGTTGGGGATTGGGGATGCATCTATTATCAAAGCTCTTTCAGTTTCTAAAGCATTTGGGAGGACAGAGACACAAGACAAAATTATTGTCCTTTGCTTATGGGTCTTGTCCTTTGCTTATGGGCCTAAGCCCATTTCCAACAAAGGTTACGGGGACCAGTGGCGAATCCAGAAATCCACTTTAACAGGGGCACAATTAGGAAGACTCCACAAAGCCATTAAAACAAAAATTGCACAAGAAAACAAAAAAGTGGTGTCAACAAGATTTGAACCCATGACCCCATATTTCCTAGTATTAAAGATTTCCAAAGGGTCTCAAGAATGTCACAATTCACCACTTTCCTGGAATTGGGAGATTTTAGGTCTTGTTGCTAAATCGAGTCTTTCATCTCAATCTATGATGCGAAAGCCTGATGCTTTAACTATTACCAAAGTATTTGACACCTTTGCTAAGACAAAATTGCGAATTGAACTTGCTGAGCAGACTCTTTTAGCTGCATTAGAGCAAGCTGCTGTATATGCTGAAAGCATTCAACACCACCACCATCATATTCAATCTCTTTTAGAAGAGGTGACTTCATGTACTCTGTGTCTCTGGGATCAAATTCCTTCATTTTATTACCATCTTTTTGGGCAATGTCATTGTTTTAATTATGAGACTCTAAGTTGGAAGGCTCTAACGTTATGTTTGCATCAGGTTTCTAAGATTGTCAAGCAGATTCATTACATGGAAGATGGTTTAGTTGAGATATACAGACGAAATGCTGAACGGAATACTGGAAAGTTCCCTGATGATGTTCTCACTGTATCAAGGTAG

Protein sequence

MSAALAVANKRKALDSFDPFPKRNSSLPLVKSEDVADLGEIKLRQNSVANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERYLRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGIGDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDTFAKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSFYYHLFGQCHCFNYETLSWKALTLCLHQVSKIVKQIHYMEDGLVEIYRRNAERNTGKFPDDVLTVSR

Homology

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BLAST of PI0011111 vs. ExPASy Swiss-Prot
Match: Q42572 (DNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=LIG1 PE=2 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 1.0e-38
Identity = 155/483 (32.09%), Postives = 205/483 (42.44%), Query Frame = 0


Query: 8   ANKRKALDSFDPFPKRNSSLPLVKSEDVADLGEIKLRQNSVA--NKRCASNVQ--ERTSK 67
           AN  K + S    PK    L     E V+D    +   +S+A  +K  A   +   +T +
Sbjct: 92  ANLGKTIVSEGTLPKTEDLL-----EPVSDSANPRSDTSSIAEDSKTGAKKAKTLSKTDE 151

Query: 68  LKSSIFELKKKAKDFNWKNVGCWKEGERY------LRFDMISKESSRITIIDIVCNMLRI 127
           +KS I  LKKK  DF+ + + CW++GER       L FD+IS ES RI I DI+CNMLR 
Sbjct: 152 MKSKIGLLKKKPNDFDPEKMSCWEKGERVPFLFVALAFDLISNESGRIVITDILCNMLRT 211

Query: 128 VMDTTPDDLVATVYLLVNKIALAHEELELGIGDASIIKALSVSKAFGRTETQDKIIVLCL 187
           V+ TTP+DLVATVYL  N+IA AHE +ELGIG+++IIKA  +S+AFGRTE   K      
Sbjct: 212 VIATTPEDLVATVYLSANEIAPAHEGVELGIGESTIIKA--ISEAFGRTEDHVK------ 271

Query: 188 WVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMT 247
                        N   GD                                         
Sbjct: 272 -----------KQNTELGD----------------------------------------- 331

Query: 248 PYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDTF---- 307
                                     LGLVAK S S+Q+MM KP+ LT+ KVFDTF    
Sbjct: 332 --------------------------LGLVAKGSRSTQTMMFKPEPLTVVKVFDTFRQIA 391

Query: 308 ---------------------------------AKTKLRIELAEQTLLAALEQAAVYAES 367
                                             + KLR+  + QT+LAAL QAAVY E 
Sbjct: 392 KESGKDSNEKKKNRMKALLVATTDCEPLYLTRLLQAKLRLGFSGQTVLAALGQAAVYNEE 451

Query: 368 IQHHHHHIQSLLEEVTSCTLCLWDQIPSF-----------YYHLFGQCHCFNYETLS--- 416
                 + +S LEE       ++  +P +            ++L   C+      +    
Sbjct: 452 HSKPPPNTKSPLEEAAKIVKQVFTVLPVYDIIVPALLSGGVWNLPKTCNFTLGVPIGPML 483

[Close]

BLAST of PI0011111 vs. ExPASy Swiss-Prot
Match: F4HPZ9 (DNA ligase 6 OS=Arabidopsis thaliana OX=3702 GN=LIG6 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.3e-09
Identity = 89/353 (25.21%), Postives = 144/353 (40.79%), Query Frame = 0


Query: 81   KNVGCWKEGE--RYLR----FDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLV 140
            K   CW+EG+   Y+     F  +  E  +I  + ++CNM R +   +P+D++  VYL  
Sbjct: 754  KEHACWREGQPAPYIHLVRTFASVESEKGKIKAMSMLCNMFRSLFALSPEDVLPAVYLCT 813

Query: 141  NKIALAHEELELGIGDASIIKALSVSKAFGRTETQD---------KIIVLCLWVLSFAYG 200
            NKIA  HE +EL IG + I  AL  +    R+  +D          +  LC         
Sbjct: 814  NKIAADHENIELNIGGSLISSALEEACGISRSTVRDMYNSLGDLGDVAQLCRQTQKLLVP 873

Query: 201  PKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKI 260
            P P+  +      R + S  TG    RL K +  K+ +      S R + +   FLV  +
Sbjct: 874  PPPLLVRDVFSTLR-KISVQTGTGSTRLKKNLIVKLMR------SCREKEIK--FLVRTL 933

Query: 261  SKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDTFAKTKLRIELAEQ 320
            ++  +             +G + ++ L +        A+ +   ++   K     EL+E 
Sbjct: 934  ARNLR-------------IGAMLRTVLPALGR-----AIVMNSFWNDHNK-----ELSES 993

Query: 321  TLLAALE---QAAVYAESI-QHHHHHIQSLLE---EVTSCTLCLWDQI---PSFYYHLFG 380
                 LE    A V A +I       + SL++   E ++ TL +   I   P       G
Sbjct: 994  CFREKLEGVSAAVVEAYNILPSLDVVVPSLMDKDIEFSTSTLSMVPGIPIKPMLAKIAKG 1053

Query: 381  QCHCFNYETLSWKALTLCLHQVSKIVKQIHYMEDGLVEIYRRNAERNTGKFPD 409
                FN   LS +    C ++      QIH + DG V I+ RN +  T +FPD
Sbjct: 1054 VQEFFN---LSQEKAFTCEYKYDGQRAQIHKLLDGTVCIFSRNGDETTSRFPD 1071

[Close]

BLAST of PI0011111 vs. ExPASy Swiss-Prot
Match: P51892 (DNA ligase 1 OS=Xenopus laevis OX=8355 GN=lig1 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.1e-08
Identity = 35/87 (40.23%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 85  CWKEGER--YL----RFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIA 144
           CW  G++  YL     F+ I +ES+R+  ++ + N LR V+  TP+DL+  +YL +N++ 
Sbjct: 433 CWCNGQKVPYLAVARTFERIEEESARLKNVETLSNFLRSVIALTPEDLLPCIYLCLNRLG 492

Query: 145 LAHEELELGIGDASIIKALSVSKAFGR 166
            A+E LELGIG+  ++KA  V++A GR
Sbjct: 493 PAYEGLELGIGETILMKA--VAQATGR 517

[Close]

BLAST of PI0011111 vs. ExPASy Swiss-Prot
Match: Q869E1 (DNA ligase 1 OS=Dictyostelium discoideum OX=44689 GN=lig1 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.3e-08
Identity = 95/377 (25.20%), Postives = 148/377 (39.26%), Query Frame = 0


Query: 86  WKEGER--YL----RFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIAL 145
           WK+GE   Y+     F+M+   SSR+ II+ + N+ R +M  +P DLV T+YL +NKI  
Sbjct: 556 WKKGEAVPYMVLAKTFEMMESTSSRLIIIEHLANLFRSIMLLSPKDLVMTIYLSINKIGP 615

Query: 146 AHEELELGIGDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWR 205
           +++  ELGIG+  +IK+L+ S                                  G    
Sbjct: 616 SYQSKELGIGEHVLIKSLAEST---------------------------------GRSVD 675

Query: 206 IQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSW 265
           + K  LT  ++G L       I  +N +   T     TP   +  + K  Q+   + LS 
Sbjct: 676 VIKQELT--EVGDL------GIIAQNSRSTQTLMGKPTP-LTIQSVFKTFQQI--ADLSG 735

Query: 266 NWEILGLVAKSSLSSQSMMRKPDALTITKVFDTFAKTKLRIELAEQTLLAALEQAAVYAE 325
                G   K  L  + ++   D  T+  +     + KLRI LAE+++L AL ++ +   
Sbjct: 736 TG---GQQKKKDLIKKLLVSCKDCETLYII--RSLQGKLRIGLAERSVLMALAKSVLVTP 795

Query: 326 SI------------QHHHHHIQSLLEEVTSCTLCLWDQIPS---FYYHLFGQCHCFN-YE 385
            I            Q      +   + V S     + Q+P+   F  HL       N   
Sbjct: 796 PIDGSGQQIFDIRKQMKQEEFEERYQNVVSKVTRAYSQLPNYDLFVPHLIAVNGIDNILS 855

Query: 386 TLSWK--------------ALTLCLHQVSKIV-----------KQIHYMEDGLVEIYRRN 416
           T S K               ++  L + S +             QIH + DG   IY RN
Sbjct: 856 TCSLKVGIPVKPMLAQPTTGISQMLDRFSDMEFTCEFKYDGERAQIHRLPDGTTHIYTRN 883

[Close]

BLAST of PI0011111 vs. ExPASy Swiss-Prot
Match: P37913 (DNA ligase 1 OS=Mus musculus OX=10090 GN=Lig1 PE=1 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-06
Identity = 86/390 (22.05%), Postives = 145/390 (37.18%), Query Frame = 0


Query: 85  CWKEGERY------LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIA 144
           CWK G++         F+ I + S+R+ +++ + N+LR V+  +P DL+  +YL +N++ 
Sbjct: 278 CWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLG 337

Query: 145 LAHEELELGIGDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQW 204
              + LELG+GD  ++KA++ +                                      
Sbjct: 338 PPQQGLELGVGDGVLLKAVAQA-------------------------------------- 397

Query: 205 RIQKSTLTGAQLGRLHKAIKTK----IAQENKKVVSTRFEPMTPYFLVLKISKGSQECHN 264
                  TG QL  +   +  K    +  EN +       P  P  +    +K    C  
Sbjct: 398 -------TGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTK---FCDI 457

Query: 265 SPLSWNWEILGLVAKSSLSSQSMMRKPDAL---------TITKVFDTFAKTKLRIELAEQ 324
           + L+               S SM +K D +         +  +        +LR+ LAEQ
Sbjct: 458 ARLT--------------GSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQ 517

Query: 325 TLLAALEQAAVYAESIQHHHHHI------------------QSLLEEVTSCTLCLWDQ-I 384
           ++LAAL QA       Q     +                  Q ++ + T C +   D+ I
Sbjct: 518 SVLAALAQAVSLTPPGQEFPTVVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRII 577

Query: 385 PSFYYHLFGQC--HCFNYETLSWK-ALTLCLHQVSKIVK------------------QIH 416
           P    H   +   HC     +  K  L      VS+++K                  QIH
Sbjct: 578 PVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIH 605

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BLAST of PI0011111 vs. ExPASy TrEMBL
Match: A0A6J1JDM0 (DNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111483595 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 4.2e-72
Identity = 225/513 (43.86%), Postives = 246/513 (47.95%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLP-------------------------LVKSEDVADLGEIKLR 69
           KRKALDS DP PK NSSL                          LVKSE+  DLGE KLR
Sbjct: 72  KRKALDSLDPPPKPNSSLALDSTPPVESQCVSSTSTLDNHGDPNLVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGKFGGKIGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKQFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPANVQSPLEEGAKIVKQVYSVLPVYDK 491

[Close]

BLAST of PI0011111 vs. ExPASy TrEMBL
Match: A0A6J1FU87 (DNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111448471 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.7e-71
Identity = 224/513 (43.66%), Postives = 245/513 (47.76%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLPL-------------------------VKSEDVADLGEIKLR 69
           KRKALDS  P PK NSSL L                         VKSE+  DLGE KLR
Sbjct: 72  KRKALDSLGPPPKPNSSLALESTPPVESQCGSSTSTLDNHGDPNPVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGNFGGKIGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKTFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPANVQSPLEEGAKIVKQVYSVLPVYDK 491

[Close]

BLAST of PI0011111 vs. ExPASy TrEMBL
Match: A0A5D3DKM3 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1754G00060 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.9e-69
Identity = 223/509 (43.81%), Postives = 245/509 (48.13%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNS-----SLPLVKSE----------------DVADLGEIKLRQNS- 69
           KRKALDS DP  K +S     S P V+S+                DV DLGE KL Q++ 
Sbjct: 73  KRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHLDVVDLGETKLGQDNG 132

Query: 70  ---------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY------ 129
                      NK CAS+VQERT+KLKSSI ELKKKAKDFN KNV CWKEGER       
Sbjct: 133 GGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKNVACWKEGERVPFLFLC 192

Query: 130 LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGIGDAS 189
           L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGIGDAS
Sbjct: 193 LGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDAS 252

Query: 190 IIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGR 249
           IIKAL  S+AFGRTE Q K                    K  GD                
Sbjct: 253 IIKAL--SEAFGRTEAQVK-----------------KQYKELGD---------------- 312

Query: 250 LHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSL 309
                                                              LGLVAK+S 
Sbjct: 313 ---------------------------------------------------LGLVAKASR 372

Query: 310 SSQSMMRKPDALTITKVFDTF-------------------------------------AK 369
           SSQSMMRKPDALTI KVFDTF                                      +
Sbjct: 373 SSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQ 432

Query: 370 TKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF------ 416
           TKLRI LAEQTLLAAL QAAVYAE       +IQS LEE +     ++  +P +      
Sbjct: 433 TKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPA 492

[Close]

BLAST of PI0011111 vs. ExPASy TrEMBL
Match: A0A5A7T1R1 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00220 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.9e-69
Identity = 223/509 (43.81%), Postives = 245/509 (48.13%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNS-----SLPLVKSE----------------DVADLGEIKLRQNS- 69
           KRKALDS DP  K +S     S P V+S+                DV DLGE KL Q++ 
Sbjct: 73  KRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHLDVVDLGETKLGQDNG 132

Query: 70  ---------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY------ 129
                      NK CAS+VQERT+KLKSSI ELKKKAKDFN KNV CWKEGER       
Sbjct: 133 GGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKNVACWKEGERVPFLFLC 192

Query: 130 LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGIGDAS 189
           L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGIGDAS
Sbjct: 193 LGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDAS 252

Query: 190 IIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGR 249
           IIKAL  S+AFGRTE Q K                    K  GD                
Sbjct: 253 IIKAL--SEAFGRTEAQVK-----------------KQYKELGD---------------- 312

Query: 250 LHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSL 309
                                                              LGLVAK+S 
Sbjct: 313 ---------------------------------------------------LGLVAKASR 372

Query: 310 SSQSMMRKPDALTITKVFDTF-------------------------------------AK 369
           SSQSMMRKPDALTI KVFDTF                                      +
Sbjct: 373 SSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQ 432

Query: 370 TKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF------ 416
           TKLRI LAEQTLLAAL QAAVYAE       +IQS LEE +     ++  +P +      
Sbjct: 433 TKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPA 492

[Close]

BLAST of PI0011111 vs. ExPASy TrEMBL
Match: A0A1S3CNQ9 (DNA ligase OS=Cucumis melo OX=3656 GN=LOC103503033 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.9e-69
Identity = 223/509 (43.81%), Postives = 245/509 (48.13%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNS-----SLPLVKSE----------------DVADLGEIKLRQNS- 69
           KRKALDS DP  K +S     S P V+S+                DV DLGE KL Q++ 
Sbjct: 73  KRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHLDVVDLGETKLGQDNG 132

Query: 70  ---------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY------ 129
                      NK CAS+VQERT+KLKSSI ELKKKAKDFN KNV CWKEGER       
Sbjct: 133 GGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKNVACWKEGERVPFXFLC 192

Query: 130 LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGIGDAS 189
           L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGIGDAS
Sbjct: 193 LGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDAS 252

Query: 190 IIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGR 249
           IIKAL  S+AFGRTE Q K                    K  GD                
Sbjct: 253 IIKAL--SEAFGRTEAQVK-----------------KQYKELGD---------------- 312

Query: 250 LHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSL 309
                                                              LGLVAK+S 
Sbjct: 313 ---------------------------------------------------LGLVAKASR 372

Query: 310 SSQSMMRKPDALTITKVFDTF-------------------------------------AK 369
           SSQSMMRKPDALTI KVFDTF                                      +
Sbjct: 373 SSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQ 432

Query: 370 TKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF------ 416
           TKLRI LAEQTLLAAL QAAVYAE       +IQS LEE +     ++  +P +      
Sbjct: 433 TKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPA 492

[Close]

BLAST of PI0011111 vs. NCBI nr
Match: XP_038900958.1 (DNA ligase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 282.7 bits (722), Expect = 5.0e-72
Identity = 225/513 (43.86%), Postives = 248/513 (48.34%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLP-------------------------LVKSEDVADLGEIKLR 69
           KRKALDS DP PK +SS+                          LVKSED ADLGE KLR
Sbjct: 72  KRKALDSLDPPPKPSSSVALESTPAVESQCGSSIANLDNHGGTHLVKSEDAADLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q++            NK CAS+VQERT+KLKSSI ELK+KAKDFN KNV CWKEGER   
Sbjct: 132 QDNGGSVEPKNGPFGNKACASSVQERTAKLKSSIPELKRKAKDFNSKNVACWKEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRI I DIVCNMLR VMDTTPDDLV+TVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRIIITDIVCNMLRTVMDTTPDDLVSTVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFDTF                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       +IQS LEE +     ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPSNIQSPLEEASKIVKQVYSILPVYDK 491

[Close]

BLAST of PI0011111 vs. NCBI nr
Match: XP_023512549.1 (DNA ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.3 bits (721), Expect = 6.6e-72
Identity = 225/513 (43.86%), Postives = 246/513 (47.95%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLP-------------------------LVKSEDVADLGEIKLR 69
           KRKALDS DP PK NSSL                          LVKSE+  DLGE KLR
Sbjct: 72  KRKALDSLDPPPKSNSSLALESTPPVESQCGSSTSTLDNHGYPNLVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGNFGGKIGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKTFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPANVQSPLEEGAKIVKQVYSVLPVYDK 491

[Close]

BLAST of PI0011111 vs. NCBI nr
Match: KAG6570819.1 (DNA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 282.3 bits (721), Expect = 6.6e-72
Identity = 225/513 (43.86%), Postives = 246/513 (47.95%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLP-------------------------LVKSEDVADLGEIKLR 69
           KRKALDS DP PK NSSL                          LVKSE+  DLGE KLR
Sbjct: 72  KRKALDSLDPRPKPNSSLALESTPPVESQCGSSTSTLDNHGDPNLVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGNFGGKVGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKTFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNVQSPLEEGAKIVKQVYSVLPVYDK 491

[Close]

BLAST of PI0011111 vs. NCBI nr
Match: XP_022985559.1 (DNA ligase 1 [Cucurbita maxima])

HSP 1 Score: 282.0 bits (720), Expect = 8.6e-72
Identity = 225/513 (43.86%), Postives = 246/513 (47.95%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLP-------------------------LVKSEDVADLGEIKLR 69
           KRKALDS DP PK NSSL                          LVKSE+  DLGE KLR
Sbjct: 72  KRKALDSLDPPPKPNSSLALDSTPPVESQCVSSTSTLDNHGDPNLVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGKFGGKIGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKQFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 --AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIPSF-- 416
              +TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P +  
Sbjct: 432 RLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPANVQSPLEEGAKIVKQVYSVLPVYDK 491

[Close]

BLAST of PI0011111 vs. NCBI nr
Match: KAG7010661.1 (DNA ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281.2 bits (718), Expect = 1.5e-71
Identity = 225/517 (43.52%), Postives = 246/517 (47.58%), Query Frame = 0


Query: 10  KRKALDSFDPFPKRNSSLPL-------------------------VKSEDVADLGEIKLR 69
           KRKALDS DP PK NSSL L                         VKSE+  DLGE KLR
Sbjct: 72  KRKALDSLDPRPKPNSSLALESTPPVESQCGSSTSTLDNHGDPNPVKSEEAVDLGETKLR 131

Query: 70  QNS----------VANKRCASNVQERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERY-- 129
           Q+           VANK+CAS+VQERT+KLKSSI ELKKKAKDFN K   CW EGER   
Sbjct: 132 QDDGGNFGGKIGPVANKKCASSVQERTAKLKSSIPELKKKAKDFNSKTFACWNEGERVPF 191

Query: 130 ----LRFDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLVNKIALAHEELELGI 189
               L FDMIS+ESSRITI DIVCNMLR VMDTTPDDLVATVYLL NKIA AHE LELGI
Sbjct: 192 LFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGI 251

Query: 190 GDASIIKALSVSKAFGRTETQDKIIVLCLWVLSFAYGPKPISNKGYGDQWRIQKSTLTGA 249
           GDASIIKAL  S+AFGRTE Q K                    K  GD            
Sbjct: 252 GDASIIKAL--SEAFGRTEAQVK-----------------KQYKELGD------------ 311

Query: 250 QLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKISKGSQECHNSPLSWNWEILGLVA 309
                                                                  LGLVA
Sbjct: 312 -------------------------------------------------------LGLVA 371

Query: 310 KSSLSSQSMMRKPDALTITKVFDTF----------------------------------- 369
           K+S SSQSMMRKPDALTITKVFD+F                                   
Sbjct: 372 KASRSSQSMMRKPDALTITKVFDSFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLI 431

Query: 370 ------AKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIP 416
                 ++TKLRI LAEQTLLAAL QAAVYAE       ++QS LEE       ++  +P
Sbjct: 432 RLLQVMSRTKLRIGLAEQTLLAALGQAAVYAEKHSTPPANVQSPLEEGAKIVKQVYSVLP 491

[Close]

BLAST of PI0011111 vs. TAIR 10
Match: AT1G08130.1 (DNA ligase 1 )

HSP 1 Score: 162.5 bits (410), Expect = 7.1e-40
Identity = 155/483 (32.09%), Postives = 205/483 (42.44%), Query Frame = 0


Query: 8   ANKRKALDSFDPFPKRNSSLPLVKSEDVADLGEIKLRQNSVA--NKRCASNVQ--ERTSK 67
           AN  K + S    PK    L     E V+D    +   +S+A  +K  A   +   +T +
Sbjct: 92  ANLGKTIVSEGTLPKTEDLL-----EPVSDSANPRSDTSSIAEDSKTGAKKAKTLSKTDE 151

Query: 68  LKSSIFELKKKAKDFNWKNVGCWKEGERY------LRFDMISKESSRITIIDIVCNMLRI 127
           +KS I  LKKK  DF+ + + CW++GER       L FD+IS ES RI I DI+CNMLR 
Sbjct: 152 MKSKIGLLKKKPNDFDPEKMSCWEKGERVPFLFVALAFDLISNESGRIVITDILCNMLRT 211

Query: 128 VMDTTPDDLVATVYLLVNKIALAHEELELGIGDASIIKALSVSKAFGRTETQDKIIVLCL 187
           V+ TTP+DLVATVYL  N+IA AHE +ELGIG+++IIKA  +S+AFGRTE   K      
Sbjct: 212 VIATTPEDLVATVYLSANEIAPAHEGVELGIGESTIIKA--ISEAFGRTEDHVK------ 271

Query: 188 WVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMT 247
                        N   GD                                         
Sbjct: 272 -----------KQNTELGD----------------------------------------- 331

Query: 248 PYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDTF---- 307
                                     LGLVAK S S+Q+MM KP+ LT+ KVFDTF    
Sbjct: 332 --------------------------LGLVAKGSRSTQTMMFKPEPLTVVKVFDTFRQIA 391

Query: 308 ---------------------------------AKTKLRIELAEQTLLAALEQAAVYAES 367
                                             + KLR+  + QT+LAAL QAAVY E 
Sbjct: 392 KESGKDSNEKKKNRMKALLVATTDCEPLYLTRLLQAKLRLGFSGQTVLAALGQAAVYNEE 451

Query: 368 IQHHHHHIQSLLEEVTSCTLCLWDQIPSF-----------YYHLFGQCHCFNYETLS--- 416
                 + +S LEE       ++  +P +            ++L   C+      +    
Sbjct: 452 HSKPPPNTKSPLEEAAKIVKQVFTVLPVYDIIVPALLSGGVWNLPKTCNFTLGVPIGPML 483

[Close]

BLAST of PI0011111 vs. TAIR 10
Match: AT1G49250.1 (ATP-dependent DNA ligase )

HSP 1 Score: 104.8 bits (260), Expect = 1.7e-22
Identity = 124/395 (31.39%), Postives = 171/395 (43.29%), Query Frame = 0


Query: 59  ERTSKLKSSIFELKKKAKDFNWKNVGCWKEGERYLRFDMISKESSRITIIDIVCNMLRIV 118
           +R +KLKS I  LKKK                          ES RI I  I+CNMLR V
Sbjct: 40  DRVTKLKSKIGLLKKK------------------------PTESGRIVITHILCNMLRTV 99

Query: 119 MDTTPDDLVATVYLLVNKIALAHEELELGIGDAS-IIKALSVSKAFGRTETQDKIIVLCL 178
           + TTPDDL+ TVYL  N+IA AHE ++LG+G  S IIKA  +S+AFGRTE+  +      
Sbjct: 100 IATTPDDLLPTVYLAANEIAPAHEGIKLGMGKGSYIIKA--ISEAFGRTESHVE------ 159

Query: 179 WVLSFAYGPKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMT 238
                                  Q+ T    QLG L       +A  ++   +  F P  
Sbjct: 160 -----------------------QQYT----QLGDL-----GLVANGSRSSQTMIFMPKP 219

Query: 239 PYFLVLKISKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDT----- 298
               V+K++               + L L+AK S    S  +K D +    V  T     
Sbjct: 220 --LTVVKVA---------------DTLRLIAKES-GKGSKDKKKDLMKALLVATTDCEPL 279

Query: 299 ----FAKTKLRIELAEQTLLAALEQAAVYAESIQHHHHHIQSLLEEVTSCTLCLWDQIP- 358
                 +  LR+  + QT+LAAL QAAVY E       +I++ L+E  +    ++  +P 
Sbjct: 280 YLTRLLQDNLRLGFSRQTVLAALGQAAVYNEEHSKPPPNIKNPLDEAATIVKEVFSMLPV 339

Query: 359 ----------SFYYHLFGQCHC---FNYETLSWKALT---LCLHQVSKIV---------- 416
                     S  ++L   C+         +  KA T   L L +    V          
Sbjct: 340 YDIIVGALLTSGVWNLPKTCNLTLGVPVRPMLAKATTRVDLILEKFKDTVFTAEYKYDGE 352

[Close]

BLAST of PI0011111 vs. TAIR 10
Match: AT1G66730.1 (DNA LIGASE 6 )

HSP 1 Score: 63.5 bits (153), Expect = 4.5e-10
Identity = 89/353 (25.21%), Postives = 144/353 (40.79%), Query Frame = 0


Query: 81   KNVGCWKEGE--RYLR----FDMISKESSRITIIDIVCNMLRIVMDTTPDDLVATVYLLV 140
            K   CW+EG+   Y+     F  +  E  +I  + ++CNM R +   +P+D++  VYL  
Sbjct: 754  KEHACWREGQPAPYIHLVRTFASVESEKGKIKAMSMLCNMFRSLFALSPEDVLPAVYLCT 813

Query: 141  NKIALAHEELELGIGDASIIKALSVSKAFGRTETQD---------KIIVLCLWVLSFAYG 200
            NKIA  HE +EL IG + I  AL  +    R+  +D          +  LC         
Sbjct: 814  NKIAADHENIELNIGGSLISSALEEACGISRSTVRDMYNSLGDLGDVAQLCRQTQKLLVP 873

Query: 201  PKPISNKGYGDQWRIQKSTLTGAQLGRLHKAIKTKIAQENKKVVSTRFEPMTPYFLVLKI 260
            P P+  +      R + S  TG    RL K +  K+ +      S R + +   FLV  +
Sbjct: 874  PPPLLVRDVFSTLR-KISVQTGTGSTRLKKNLIVKLMR------SCREKEIK--FLVRTL 933

Query: 261  SKGSQECHNSPLSWNWEILGLVAKSSLSSQSMMRKPDALTITKVFDTFAKTKLRIELAEQ 320
            ++  +             +G + ++ L +        A+ +   ++   K     EL+E 
Sbjct: 934  ARNLR-------------IGAMLRTVLPALGR-----AIVMNSFWNDHNK-----ELSES 993

Query: 321  TLLAALE---QAAVYAESI-QHHHHHIQSLLE---EVTSCTLCLWDQI---PSFYYHLFG 380
                 LE    A V A +I       + SL++   E ++ TL +   I   P       G
Sbjct: 994  CFREKLEGVSAAVVEAYNILPSLDVVVPSLMDKDIEFSTSTLSMVPGIPIKPMLAKIAKG 1053

Query: 381  QCHCFNYETLSWKALTLCLHQVSKIVKQIHYMEDGLVEIYRRNAERNTGKFPD 409
                FN   LS +    C ++      QIH + DG V I+ RN +  T +FPD
Sbjct: 1054 VQEFFN---LSQEKAFTCEYKYDGQRAQIHKLLDGTVCIFSRNGDETTSRFPD 1071

The following BLAST results are available for this feature:

BLAST of PI0011111 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42572	1.0e-38	32.09	DNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=LIG1 PE=2 SV=2	[more]
F4HPZ9	6.3e-09	25.21	DNA ligase 6 OS=Arabidopsis thaliana OX=3702 GN=LIG6 PE=2 SV=1	[more]
P51892	3.1e-08	40.23	DNA ligase 1 OS=Xenopus laevis OX=8355 GN=lig1 PE=2 SV=1	[more]
Q869E1	5.3e-08	25.20	DNA ligase 1 OS=Dictyostelium discoideum OX=44689 GN=lig1 PE=3 SV=1	[more]
P37913	2.2e-06	22.05	DNA ligase 1 OS=Mus musculus OX=10090 GN=Lig1 PE=1 SV=2	[more]

BLAST of PI0011111 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDM0	4.2e-72	43.86	DNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111483595 PE=3 SV=1	[more]
A0A6J1FU87	2.7e-71	43.66	DNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111448471 PE=3 SV=1	[more]
A0A5D3DKM3	1.9e-69	43.81	DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1754G00060 PE...	[more]
A0A5A7T1R1	1.9e-69	43.81	DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00220 PE=3 ...	[more]
A0A1S3CNQ9	1.9e-69	43.81	DNA ligase OS=Cucumis melo OX=3656 GN=LOC103503033 PE=3 SV=1	[more]

BLAST of PI0011111 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900958.1	5.0e-72	43.86	DNA ligase 1 isoform X1 [Benincasa hispida]	[more]
XP_023512549.1	6.6e-72	43.86	DNA ligase 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6570819.1	6.6e-72	43.86	DNA ligase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022985559.1	8.6e-72	43.86	DNA ligase 1 [Cucurbita maxima]	[more]
KAG7010661.1	1.5e-71	43.52	DNA ligase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of PI0011111 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08130.1	7.1e-40	32.09	DNA ligase 1	[more]
AT1G49250.1	1.7e-22	31.39	ATP-dependent DNA ligase	[more]
AT1G66730.1	4.5e-10	25.21	DNA LIGASE 6	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012308	DNA ligase, ATP-dependent, N-terminal	PFAM	PF04675	DNA_ligase_A_N	coord: 95..295 e-value: 5.1E-10 score: 40.0
IPR036599	DNA ligase, ATP-dependent, N-terminal domain superfamily	GENE3D	1.10.3260.10		coord: 70..173 e-value: 6.4E-18 score: 67.2
IPR036599	DNA ligase, ATP-dependent, N-terminal domain superfamily	SUPERFAMILY	117018	ATP-dependent DNA ligase DNA-binding domain	coord: 94..162
IPR036599	DNA ligase, ATP-dependent, N-terminal domain superfamily	SUPERFAMILY	117018	ATP-dependent DNA ligase DNA-binding domain	coord: 264..336
None	No IPR available	PANTHER	PTHR45674:SF4	DNA LIGASE 1	coord: 382..411
None	No IPR available	PANTHER	PTHR45674	DNA LIGASE 1/3 FAMILY MEMBER	coord: 264..294 coord: 295..348 coord: 46..169
None	No IPR available	PANTHER	PTHR45674	DNA LIGASE 1/3 FAMILY MEMBER	coord: 382..411
None	No IPR available	PANTHER	PTHR45674:SF4	DNA LIGASE 1	coord: 264..294
None	No IPR available	PANTHER	PTHR45674:SF4	DNA LIGASE 1	coord: 295..348 coord: 46..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0011111.1	PI0011111.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003910	DNA ligase (ATP) activity