PI0010853 (gene) Melon (PI 482460) v1

Overview
NamePI0010853
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionnephrocystin-3
Locationchr09: 224450 .. 229217 (+)
RNA-Seq ExpressionPI0010853
SyntenyPI0010853
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCTTTATCGAGCTATCGTTCACCACGAGCTGAGAAGAGCTTTTGTAACTGTAGCTGCTCAACTCTCTACAATGGCGACTTTCCTTCTTCTCTCTTCTCCATCTTACACCTGTTACAGGTTTTTTTTCTTTTCCTTATCTACATCCCAACATTTTGGCAACTTTCATCTCTTCTTTTACTTCTTTTCTTTCCGGCGTTTTTCTGTTATATTGGTAGCTACTGTCTATTGCATTTGTTTCAAAATCAGTATATGTATCCACTGCTGTAAAATAAATATGCTGTATGCAGTTTTAGGAGACCGATTATTCTAATATGTCTTTCTCGAACATCTTAGGATGAGTGAAATCTGTTTTTTCCGTGCAGTCATTGCATTTGCAAAAGGGCATCAATCACATGCTCCTCAGTTTGCCTTCAGAAACCAAAATGTAATATCAAGCTTTATGCGGTACCTGTTGGAGCTTTTTCTTGTCTCTCTGCATCAACACCTTCTGCTTCTAGTAGAGCTGCTTTTGGGGCTCAAAGGAAGCACGTTTCTTCTGCGTTCACTGCTCCAAATGGATATCAAAGGTTGGGACCAAATTTTGTAAATTTCTTGCTGTCAGTGTCAAGAATTGTCTTTGATATCCAGATGAAAAATTGAACCAAGGTTGTTTACTTTTTACTAGGTCTGATCACATGCACGATGGGAACACATTGGCTGAATTTGAAGTTCAGTTGGAAGAATTACTCAATGAAGTCAGAATGATGACCATGAGCGGGAGGAAGAATGATGCTGTAGAGCTACTTCAAGCTAATTTTGAAGCTGTAAAAGAACAAATGGAATCAGGCGCCGTTGGCTTTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTGTATTGGATATAGTAACTTCTTTTTCCCTTATCATCCGTACAATAATTGATTTGGAAACTTTAGTGCTCCTCAATTTTGTGCAACAACGGTTGTACTTCCATGACTCGAGGATTTTATTCTTTTGTCTAGGTTATTATTTAGCCTTCATGTGGTAAAATATTAATTTAATATCCTATAAACTGGCATTAGTTGTTTGGAGTTGCCCATGCTGGGTTGACTTGTATGTAGACCGGGTCAGGAGTCAGGACCATGGAATATAGGACTTGGAAGTTCTTTTTCCTCTTGATGTGATGACAGTTTAATTCTGTTTTAAAAATTATACGAGCATGTCAGGGCCTTATTCTTGCTTAAAGACAAGTGGAGATGGTCATCTAGCAATCTAGTTACATTTTTTAGGCGATTTTGAAGTAATAATGAAGTAGCTTATAGCTTAAAAATTATACGAGCATGTCTGGACCTTAGTTTTATGCTCCAAAAGTATCCCATGCTTATACATGCAAAGTTTCAGCTGGTCTATCTTCTAGACTCACTTCGGTTTAAGTGAGATTCATTTCCTTCCTCTAATGGATTTTCCTTCATGCAGTTGAACAAGGTTGTTGACAGTCTGACGGACGGTGAACCCTTTCTGGATTCAGTGCTTTTGCATATGGGGAGCATGTACTCAACCTTAAAAAAGTTTGAGAAATCAAAATCTGCGTACAAGAGGGCTATTGATATCATAGAGAAAAAAAGTGGTACGGGAGCTATAATTTGTTTTTCTATTCCTCTCTAAACCGTCTTACAATGAAACCCTTGCAGGAGAAGACAGTAGCTTTCTTATCACTCCAATTTTAGGGATGGCTAAAGTTCTTGGTACCATTGGAAGAGCTGGAAAGGCAGTAGAGTTTTACAATCGTGCAATTTCAATTCTGGAATCAAAGAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTGTCTAGTCTGGGCAATCTTATGCTCAAAGAAGGAAAAGGCAAGGATGCAGAAACATGTTTTGCAAGGTATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTCTCTCTCTCTCTCTATCTCTATCTCTTGCTTTGGACTATTCACTTACTTTCATGGCCCTATTTAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAAATGGAAAAGTCGTAATGGCTATGTGTTCTCTGGCTAATGCAAAATGTGCAAAAGGTAACTTTGGTCGCTTGTGTCATCTTTTTATTTTTATACACTAGCTAAAATTTTCATTTGCAAAATATTCTTCCACTTGTTCACAGGGGAAGCAGACGAAGCTGTTACCTTATACAGAAGAGCTTTGGAGATTATCAAGGATTCAAATGATTTGGCTTTAGATGACAGCACCATTGAGAAGATGAGGATTGATTTGGCAGAACTATTGCATGTTCTAGGAAGGTATTGTCATACACATACTTATGTTGGGATTTTCTATTAAAAAAACTGTTGGCGTTTGAAGCTTTTCACTATTGATAGAGCCGAAGTTACTGGAATGTATTGAAAGATATGAAATAGAAAAACATTAAAAATTCCCAAGTAATGAAGGTACTTGACCCTCCTTAAACCAATTGCCTGCCTTCCTCTCATCCCATTCCTTCTATATTATTTCTTGGGTTAATAATTAACACGCTTCTATTATTCGAATAATGTTCCTAACCTCCCAAGAGCATTCCTAGCTCTTTTCTTTTACCACCAGTATTCATGAGTTCCTAGAATTTGGTTTAATACAGAGGGAATGAAGGCAGAGAACTTCTAGAAGAGTGTTTGTTGGTCAATGAAAGGTTGAAAGGAAAAGAGCATCCCAGCTCAGTGAAGCACCTTGTAAACCTTGCCGCTTCTTATTCACGGTCAAAGAATTATGTGGAGGCTGCGCGTTTGTTGCGAATTGGATTGGACATTATGATAAAGGCAGTGGGATCTGATGATCAATCAATTACAGTCCCAATGTTGAATCTTGCTGTCACTCTTTACAATCTAAAACAAGACGATGATGCGGAGCAACTTGTGCTTGAAGTCTTGCGAATAAGGGAAAATGCTTTTGGAAAAGATTCACTTCCTGTTGGTGAGAATTGATAATTCCTACTTGATAGATAATGACGTTTATATCATTGTTTTATCATCTTCACATGAGCTCCTTTTTTTGCTGAACTGCACGCAGGTGAGGCTCTAGATTGTTTGGTTTCGATTCAGAACAGGCTAGGGAAGGATGAAAGTGAGCTACTGAAGCTGCTGAAGAGAATTCTTAGAATCCAGGAGAGAGAGTTCGGGGATGATAGTAAAGAGGTCATCGACACCCTCAAGAAAATAGCATTCTACATGGAAAAACTGGGAATGAAAGATGAGAAATTTCTACTTCAAAAACGACTATCCATGCTGCGGATAAAATTCAGGAACCAGATGCAATACTAAAGGTATTTAACATTCATCTTTCATAATCTTGTGATATATATTTTCCTTTTCTTTAATGATACAGCTTCTGGATTAATTGTTTAGATAGATTTATATAATGTTCCTAAACTTTAAAGAGAAAGAGGTCTTAATACACGTCTCCATCTCTAAATGATGATTCTATGTGATAAATATATTTGATACCTTGCTTAAAATAACAATCGAATGTTGTCAGTGCATGACAATGTTATATTTTTTAAATACTTTATTTTTCTTTAATTTTTTAAAGTAATATTTTTCTCTTATTTCGCCTCCCCCTCCCCCTGCGGCCATCGGGGCTGCTGCCCTATCTCTCTCTCTCTCTCTCTCTTTTTTTCCTTTCTTCTCTTTCTCATCATTTCTCTCCTTCTCCACAATTACCAAAAAGGAAATGTAACGAACCAACGTTTTAAAACCTCCAGCTGGAGTCATACATGACATTTCCAATATAAAACCTCAACTTACACTTTTTCGTGGCAAGGTATGTCTTATCCGTAAAAACTTTTTGGTAGAACATTGTGCATAAAAGTATTTGTTTAGCTTGATGGACCAGTAGGTTTATCATTGCTTGTTCATTGACTTGATGAAAAATAGTTATGTGTTGGTTGGTAGGCTATGGCGCCACGCAAAATAGGTTCAACTCAAAGTAGATGAGGTAGGCCCGCTAGAGGCTAGCCAGTGCTAGGGTCACTGGAACAACCACTCAAGAGCCGAGCAAATGGCCAGGCAAGGGCTCCCACCAGTGGTCCAACCACCATTGACTCTACTCGGTAACCTGATTGAATTTGCCTCTTAAACTTTGACTGCCGTCATGATGGAGAGAATGCAATCATTGTTCCAGACTACAATCACCAACTAGATGGCCTAGTTGAATATAAGTCAACAAAACATGTGAGAGGAAGCGAAACGGTTATGAGATTTCAAGAAGTACAACTTGAAGTTTTGATAAGTCACTAGTTGTTGTTGATAAAACTATCAACGAATATTTGGTTATATGAAATGCCTTAGAGTGTCAGAAATTGCAGTGACATTGTGTTGATGGAATTGGACAATGCCTATATATGGTGTGGGAGTCCGTCGAGAGGTTGATTGACACTAGCGAAGGCATCGTGACTTGGTCACAGTTTAAAGAAGCCTTCAACAAGCAGAGCTTTGCTACAGTAACAAGAGTTAACAATCAACAGAGTTCTTGAACTTTAAACAGGTAAATTGATCGGTTGATGAGTATGAAAGAAAATTTAACAATTGCCACAAAAAGAGTTTGATTATGCTTGGAGCTTGAAATGTTATTGTTGATTTGTCTTTA

mRNA sequence

CCCCTTTATCGAGCTATCGTTCACCACGAGCTGAGAAGAGCTTTTGTAACTGTAGCTGCTCAACTCTCTACAATGGCGACTTTCCTTCTTCTCTCTTCTCCATCTTACACCTGTTACAGTCATTGCATTTGCAAAAGGGCATCAATCACATGCTCCTCAGTTTGCCTTCAGAAACCAAAATGTAATATCAAGCTTTATGCGGTACCTGTTGGAGCTTTTTCTTGTCTCTCTGCATCAACACCTTCTGCTTCTAGTAGAGCTGCTTTTGGGGCTCAAAGGAAGCACGTTTCTTCTGCGTTCACTGCTCCAAATGGATATCAAAGGTCTGATCACATGCACGATGGGAACACATTGGCTGAATTTGAAGTTCAGTTGGAAGAATTACTCAATGAAGTCAGAATGATGACCATGAGCGGGAGGAAGAATGATGCTGTAGAGCTACTTCAAGCTAATTTTGAAGCTGTAAAAGAACAAATGGAATCAGGCGCCGTTGGCTTTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTGTATTGGATATATTGAACAAGGTTGTTGACAGTCTGACGGACGGTGAACCCTTTCTGGATTCAGTGCTTTTGCATATGGGGAGCATGTACTCAACCTTAAAAAAGTTTGAGAAATCAAAATCTGCGTACAAGAGGGCTATTGATATCATAGAGAAAAAAAGTGGAGAAGACAGTAGCTTTCTTATCACTCCAATTTTAGGGATGGCTAAAGTTCTTGGTACCATTGGAAGAGCTGGAAAGGCAGTAGAGTTTTACAATCGTGCAATTTCAATTCTGGAATCAAAGAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTGTCTAGTCTGGGCAATCTTATGCTCAAAGAAGGAAAAGGCAAGGATGCAGAAACATGTTTTGCAAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAAATGGAAAAGTCGTAATGGCTATGTGTTCTCTGGCTAATGCAAAATGTGCAAAAGGGGAAGCAGACGAAGCTGTTACCTTATACAGAAGAGCTTTGGAGATTATCAAGGATTCAAATGATTTGGCTTTAGATGACAGCACCATTGAGAAGATGAGGATTGATTTGGCAGAACTATTGCATGTTCTAGGAAGAGGGAATGAAGGCAGAGAACTTCTAGAAGAGTGTTTGTTGGTCAATGAAAGGTTGAAAGGAAAAGAGCATCCCAGCTCAGTGAAGCACCTTGTAAACCTTGCCGCTTCTTATTCACGGTCAAAGAATTATGTGGAGGCTGCGCGTTTGTTGCGAATTGGATTGGACATTATGATAAAGGCAGTGGGATCTGATGATCAATCAATTACAGTCCCAATGTTGAATCTTGCTGTCACTCTTTACAATCTAAAACAAGACGATGATGCGGAGCAACTTGTGCTTGAAGTCTTGCGAATAAGGGAAAATGCTTTTGGAAAAGATTCACTTCCTGTTGGTGAGGCTCTAGATTGTTTGGTTTCGATTCAGAACAGGCTAGGGAAGGATGAAAGTGAGCTACTGAAGCTGCTGAAGAGAATTCTTAGAATCCAGGAGAGAGAGTTCGGGGATGATAGTAAAGAGGTCATCGACACCCTCAAGAAAATAGCATTCTACATGGAAAAACTGGGAATGAAAGATGAGAAATTTCTACTTCAAAAACGACTATCCATGCTGCGGATAAAATTCAGGAACCAGATGCAATACTAAAGGCTATGGCGCCACGCAAAATAGGTTCAACTCAAAGTAGATGAGGTAGGCCCGCTAGAGGCTAGCCAGTGCTAGGGTCACTGGAACAACCACTCAAGAGCCGAGCAAATGGCCAGGCAAGGGCTCCCACCAGTGGTCCAACCACCATTGACTCTACTCGGTAACCTGATTGAATTTGCCTCTTAAACTTTGACTGCCGTCATGATGGAGAGAATGCAATCATTGTTCCAGACTACAATCACCAACTAGATGGCCTAGTTGAATATAAGTCAACAAAACATGTGAGAGGAAGCGAAACGGTTATGAGATTTCAAGAAGTACAACTTGAAGTTTTGATAAGTCACTAGTTGTTGTTGATAAAACTATCAACGAATATTTGGTTATATGAAATGCCTTAGAGTGTCAGAAATTGCAGTGACATTGTGTTGATGGAATTGGACAATGCCTATATATGGTGTGGGAGTCCGTCGAGAGGTTGATTGACACTAGCGAAGGCATCGTGACTTGGTCACAGTTTAAAGAAGCCTTCAACAAGCAGAGCTTTGCTACAGTAACAAGAGTTAACAATCAACAGAGTTCTTGAACTTTAAACAGGTAAATTGATCGGTTGATGAGTATGAAAGAAAATTTAACAATTGCCACAAAAAGAGTTTGATTATGCTTGGAGCTTGAAATGTTATTGTTGATTTGTCTTTA

Coding sequence (CDS)

ATGGCGACTTTCCTTCTTCTCTCTTCTCCATCTTACACCTGTTACAGTCATTGCATTTGCAAAAGGGCATCAATCACATGCTCCTCAGTTTGCCTTCAGAAACCAAAATGTAATATCAAGCTTTATGCGGTACCTGTTGGAGCTTTTTCTTGTCTCTCTGCATCAACACCTTCTGCTTCTAGTAGAGCTGCTTTTGGGGCTCAAAGGAAGCACGTTTCTTCTGCGTTCACTGCTCCAAATGGATATCAAAGGTCTGATCACATGCACGATGGGAACACATTGGCTGAATTTGAAGTTCAGTTGGAAGAATTACTCAATGAAGTCAGAATGATGACCATGAGCGGGAGGAAGAATGATGCTGTAGAGCTACTTCAAGCTAATTTTGAAGCTGTAAAAGAACAAATGGAATCAGGCGCCGTTGGCTTTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTGTATTGGATATATTGAACAAGGTTGTTGACAGTCTGACGGACGGTGAACCCTTTCTGGATTCAGTGCTTTTGCATATGGGGAGCATGTACTCAACCTTAAAAAAGTTTGAGAAATCAAAATCTGCGTACAAGAGGGCTATTGATATCATAGAGAAAAAAAGTGGAGAAGACAGTAGCTTTCTTATCACTCCAATTTTAGGGATGGCTAAAGTTCTTGGTACCATTGGAAGAGCTGGAAAGGCAGTAGAGTTTTACAATCGTGCAATTTCAATTCTGGAATCAAAGAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTGTCTAGTCTGGGCAATCTTATGCTCAAAGAAGGAAAAGGCAAGGATGCAGAAACATGTTTTGCAAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAAATGGAAAAGTCGTAATGGCTATGTGTTCTCTGGCTAATGCAAAATGTGCAAAAGGGGAAGCAGACGAAGCTGTTACCTTATACAGAAGAGCTTTGGAGATTATCAAGGATTCAAATGATTTGGCTTTAGATGACAGCACCATTGAGAAGATGAGGATTGATTTGGCAGAACTATTGCATGTTCTAGGAAGAGGGAATGAAGGCAGAGAACTTCTAGAAGAGTGTTTGTTGGTCAATGAAAGGTTGAAAGGAAAAGAGCATCCCAGCTCAGTGAAGCACCTTGTAAACCTTGCCGCTTCTTATTCACGGTCAAAGAATTATGTGGAGGCTGCGCGTTTGTTGCGAATTGGATTGGACATTATGATAAAGGCAGTGGGATCTGATGATCAATCAATTACAGTCCCAATGTTGAATCTTGCTGTCACTCTTTACAATCTAAAACAAGACGATGATGCGGAGCAACTTGTGCTTGAAGTCTTGCGAATAAGGGAAAATGCTTTTGGAAAAGATTCACTTCCTGTTGGTGAGGCTCTAGATTGTTTGGTTTCGATTCAGAACAGGCTAGGGAAGGATGAAAGTGAGCTACTGAAGCTGCTGAAGAGAATTCTTAGAATCCAGGAGAGAGAGTTCGGGGATGATAGTAAAGAGGTCATCGACACCCTCAAGAAAATAGCATTCTACATGGAAAAACTGGGAATGAAAGATGAGAAATTTCTACTTCAAAAACGACTATCCATGCTGCGGATAAAATTCAGGAACCAGATGCAATACTAA

Protein sequence

MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSASSRAAFGAQRKHVSSAFTAPNGYQRSDHMHDGNTLAEFEVQLEELLNEVRMMTMSGRKNDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDSLTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEKLGMKDEKFLLQKRLSMLRIKFRNQMQY
Homology
BLAST of PI0010853 vs. ExPASy Swiss-Prot
Match: P0CI65 (Nephrocystin-3 OS=Danio rerio OX=7955 GN=nphp3 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 3.5e-18
Identity = 101/384 (26.30%), Postives = 165/384 (42.97%), Query Frame = 0

Query: 176  DSLTDGEPFLDS--VLLHMGSMYSTLKKFEK-------SKSAYKRAIDIIEKKSGEDSSF 235
            D+L + E   +S   +  + ++Y TL +F K       + +  +R+++I E     D   
Sbjct: 902  DALKEFEKTCESEQSMSRLANLYETLGRFLKDLGLLSQAVAPLQRSLEIRETALDPDHPS 961

Query: 236  LITPILGMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKG 295
            +   +  +A V     + G A + Y +A+ I E+  G E+  +   L SL  L  K+ K 
Sbjct: 962  VAQSLHQLAGVYVHWRKFGNAEQLYKQAMEICENAYGPEHSTVARELDSLSLLYQKQNKY 1021

Query: 296  KDAETCFARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSN 355
            + AE    R V I +K   +K      A+                  L RRAL++  +  
Sbjct: 1022 EQAEKLRKRSVKIRQKTARQKGHMYGFAL------------------LKRRALQL--EEL 1081

Query: 356  DLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAA 415
             L  D +   K   +L  L ++    +  +  L   L + +R+ G +HP   + L NLAA
Sbjct: 1082 TLGKDSTDCAKTLNELGVLYYLQNNLDAAKLFLTRSLEMRQRVLGPDHPDCAQSLNNLAA 1141

Query: 416  SYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEV 475
             +S  K Y  A  L    LDI  +A+  D  S+   + +LA+      + + A  L    
Sbjct: 1142 LHSERKEYESAEELYERALDIRKRALAPDHPSLAYTLKHLAMLYKRRGKLEKAVPLYELA 1201

Query: 476  LRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVID 535
            L IRE +FG     V  AL  L  +  +L K  S+ L L +R L++ E   G     V +
Sbjct: 1202 LEIREKSFGPKHPSVATALVNLAVLYCQL-KQHSDALPLYERALKVYEDSLGRLHPRVGE 1261

Query: 536  TLKKIAFYMEKLGMKDEKFLLQKR 551
            TLK +A    + G  ++   L KR
Sbjct: 1262 TLKNLAVLSYEEGDFEKAAELYKR 1264

BLAST of PI0010853 vs. ExPASy Swiss-Prot
Match: Q07866 (Kinesin light chain 1 OS=Homo sapiens OX=9606 GN=KLC1 PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 2.4e-11
Identity = 55/204 (26.96%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 324 AKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECL 383
           ++G  + AV L ++ALE ++ ++    D   +  M   LA +     +  +   LL + L
Sbjct: 225 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKDAANLLNDAL 284

Query: 384 LVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPM 443
            + E+  GK+HP+    L NLA  Y +   Y EA  L +  L+I  K +G D   +   +
Sbjct: 285 AIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL 344

Query: 444 LNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELL 503
            NLA+   N  + ++ E      L I +   G D   V +  + L S   + GK +    
Sbjct: 345 NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAET 404

Query: 504 KLLKRILRIQEREFG---DDSKEV 525
              + + R  EREFG   D++K +
Sbjct: 405 LYKEILTRAHEREFGSVDDENKPI 426

BLAST of PI0010853 vs. ExPASy Swiss-Prot
Match: Q5R581 (Kinesin light chain 1 OS=Pongo abelii OX=9601 GN=KLC1 PE=2 SV=3)

HSP 1 Score: 72.0 bits (175), Expect = 2.4e-11
Identity = 55/204 (26.96%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 324 AKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECL 383
           ++G  + AV L ++ALE ++ ++    D   +  M   LA +     +  +   LL + L
Sbjct: 225 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKDAANLLNDAL 284

Query: 384 LVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPM 443
            + E+  GK+HP+    L NLA  Y +   Y EA  L +  L+I  K +G D   +   +
Sbjct: 285 AIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL 344

Query: 444 LNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELL 503
            NLA+   N  + ++ E      L I +   G D   V +  + L S   + GK +    
Sbjct: 345 NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAET 404

Query: 504 KLLKRILRIQEREFG---DDSKEV 525
              + + R  EREFG   D++K +
Sbjct: 405 LYKEILTRAHEREFGSVDDENKPI 426

BLAST of PI0010853 vs. ExPASy Swiss-Prot
Match: P46825 (Kinesin light chain OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.1e-11
Identity = 55/204 (26.96%), Postives = 94/204 (46.08%), Query Frame = 0

Query: 324 AKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECL 383
           ++G  + AV L ++ALE ++ ++    D   +  M   LA +    G+  E   LL + L
Sbjct: 232 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQGKYKEAANLLNDAL 291

Query: 384 LVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPM 443
            + E+  G +HP+    L NLA  Y +   Y +A  L +  L I  K +G D   +   +
Sbjct: 292 GIREKTLGPDHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALVIREKVLGKDHPDVAKQL 351

Query: 444 LNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELL 503
            NLA+   N  + ++ E+     L I +   G D   V +  + L S   + GK +   +
Sbjct: 352 NNLALLCQNQGKYEEVERYYQRALEIYQKELGPDDPNVAKTKNNLASAYLKQGKYKQAEI 411

Query: 504 KLLKRILRIQEREFG---DDSKEV 525
              + + R  E+EFG   DD+K +
Sbjct: 412 LYKEVLTRAHEKEFGKVDDDNKPI 433

BLAST of PI0010853 vs. ExPASy Swiss-Prot
Match: P37285 (Kinesin light chain 1 OS=Rattus norvegicus OX=10116 GN=Klc1 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 5.4e-11
Identity = 54/204 (26.47%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 324 AKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECL 383
           ++G  + AV L ++ALE ++ ++    D   +  M   LA +     +  +   LL + L
Sbjct: 225 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKDAANLLNDAL 284

Query: 384 LVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPM 443
            + E+  G++HP+    L NLA  Y +   Y EA  L +  L+I  K +G D   +   +
Sbjct: 285 AIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL 344

Query: 444 LNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESELL 503
            NLA+   N  + ++ E      L I +   G D   V +  + L S   + GK +    
Sbjct: 345 NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAET 404

Query: 504 KLLKRILRIQEREFG---DDSKEV 525
              + + R  EREFG   D++K +
Sbjct: 405 LYKEILTRAHEREFGSVDDENKPI 426

BLAST of PI0010853 vs. ExPASy TrEMBL
Match: A0A1S3AV29 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103482972 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.6e-284
Identity = 523/567 (92.24%), Postives = 541/567 (95.41%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSAS 60
           MAT LLLSSPSYTCYSHCICKR SITCSSVCLQKP CNIKLYA P GAFSCLSASTPSAS
Sbjct: 1   MATSLLLSSPSYTCYSHCICKRPSITCSSVCLQKPLCNIKLYAGPFGAFSCLSASTPSAS 60

Query: 61  SRAAFGAQRKHVSSAFTAPNGYQRSDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRK 120
           SRAAFGAQRKHV SAFTAP GYQRSDHMH   D +T+AE EVQLEELLNEVRMMTMSGRK
Sbjct: 61  SRAAFGAQRKHVFSAFTAPIGYQRSDHMHYRTDSDTVAELEVQLEELLNEVRMMTMSGRK 120

Query: 121 NDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDS 180
           NDAVELLQAN+E VKEQMESGAVG EQAA+LDIVALGY+TVGDLKFVASVLDILNKVVDS
Sbjct: 121 NDAVELLQANYEVVKEQMESGAVGIEQAALLDIVALGYLTVGDLKFVASVLDILNKVVDS 180

Query: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKV 240
           LTDGEPFLDSVLLHMGSMYSTLKKFEKS SAYKRAIDI+EKK GEDSSFLITPILGMAKV
Sbjct: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSVSAYKRAIDIMEKKFGEDSSFLITPILGMAKV 240

Query: 241 LGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIV 300
           LGTIGRAGKAVE YNRAIS+LES+RGFENEDLVIPL SLGNLMLKEGKGKDAETCFARIV
Sbjct: 241 LGTIGRAGKAVECYNRAISLLESRRGFENEDLVIPLFSLGNLMLKEGKGKDAETCFARIV 300

Query: 301 NIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEK 360
           NIYKKLYGEKNGKV MAM SLANAK A+GEADEAVTLYRRALEIIKDSND+A+DDST EK
Sbjct: 301 NIYKKLYGEKNGKVGMAMYSLANAKYARGEADEAVTLYRRALEIIKDSNDMAVDDSTTEK 360

Query: 361 MRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420
           MRIDLAELLHVLGRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSKNYVEA
Sbjct: 361 MRIDLAELLHVLGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420

Query: 421 ARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKD 480
            RLLRIGLDIMIKAVGSDDQSITVPML+LAVTLYNLKQDD+AEQL LEVLRIRENAFGKD
Sbjct: 421 ERLLRIGLDIMIKAVGSDDQSITVPMLDLAVTLYNLKQDDEAEQLALEVLRIRENAFGKD 480

Query: 481 SLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEK 540
           SLPVGEALDCLVSIQ+RLGKDESELLKLLKRILRIQEREFGDD +EVIDTLKKIAF MEK
Sbjct: 481 SLPVGEALDCLVSIQSRLGKDESELLKLLKRILRIQEREFGDDGEEVIDTLKKIAFCMEK 540

Query: 541 LGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           LGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 LGMKDEKFLLQKRLSMLRMKFKNQMQY 567

BLAST of PI0010853 vs. ExPASy TrEMBL
Match: A0A5D3C290 (Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00400 PE=4 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.5e-261
Identity = 492/567 (86.77%), Postives = 509/567 (89.77%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSAS 60
           MAT LLLSSPSYTCYSHCICKR SITCSSVCLQKP CNIKLYA P GAFSCLSASTPSAS
Sbjct: 1   MATSLLLSSPSYTCYSHCICKRPSITCSSVCLQKPLCNIKLYAGPFGAFSCLSASTPSAS 60

Query: 61  SRAAFGAQRKHVSSAFTAPNGYQRSDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRK 120
           SRAAFGAQRKHV SAFTAP GYQRSDHMH   D +T+AE EVQLEELLNEVRMMTMSGRK
Sbjct: 61  SRAAFGAQRKHVFSAFTAPIGYQRSDHMHYRTDSDTVAELEVQLEELLNEVRMMTMSGRK 120

Query: 121 NDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDS 180
           NDAVELLQAN+E VKEQMESGAVG EQAA+LDIVALGY+TVGDLKFVASVLDILNKVVDS
Sbjct: 121 NDAVELLQANYEVVKEQMESGAVGIEQAALLDIVALGYLTVGDLKFVASVLDILNKVVDS 180

Query: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKV 240
           LTDGEPFLDSVLLHMGSMYSTLKKFEKS SAYKRAIDI+EKK GEDSSFLITPILGMAKV
Sbjct: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSVSAYKRAIDIMEKKFGEDSSFLITPILGMAKV 240

Query: 241 LGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIV 300
           LGTIGRAGKAVE YNRAIS+LES+RGFENEDLVIPL SLGNLMLKEGKGKDAET      
Sbjct: 241 LGTIGRAGKAVECYNRAISLLESRRGFENEDLVIPLFSLGNLMLKEGKGKDAET------ 300

Query: 301 NIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEK 360
                                        EADEAVTLYRRALEIIKDSND+A+DDST EK
Sbjct: 301 ----------------------------WEADEAVTLYRRALEIIKDSNDMAVDDSTTEK 360

Query: 361 MRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420
           MRIDLAELLHVLGRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSKNYVEA
Sbjct: 361 MRIDLAELLHVLGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420

Query: 421 ARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKD 480
            RLLRIGLDIMIKAVGSDDQSITVPML+LAVTLYNLKQDD+AEQL LEVLRIRENAFGKD
Sbjct: 421 ERLLRIGLDIMIKAVGSDDQSITVPMLDLAVTLYNLKQDDEAEQLALEVLRIRENAFGKD 480

Query: 481 SLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEK 540
           SLPVGEALDCLVSIQ+RLGKDESELLKLLKRILRIQEREFGDD +EVIDTLKKIAF MEK
Sbjct: 481 SLPVGEALDCLVSIQSRLGKDESELLKLLKRILRIQEREFGDDGEEVIDTLKKIAFCMEK 533

Query: 541 LGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           LGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 LGMKDEKFLLQKRLSMLRMKFKNQMQY 533

BLAST of PI0010853 vs. ExPASy TrEMBL
Match: A0A6J1H2E0 (nephrocystin-3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.7e-249
Identity = 467/576 (81.08%), Postives = 512/576 (88.89%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCY---SHCICKRA-----SITCSSVCLQKPKCNIKLYAVPVGAFSCL 60
           MA FLLLSSPS+T +     C+C ++      ITCSSV LQK KCNIKLYAVPV AFSC 
Sbjct: 1   MAAFLLLSSPSFTYHRMSEICLCMQSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCQ 60

Query: 61  SASTPSASSRAAFGAQRKHVSSAFTAPNGYQ-RSDHMH---DGNTLAEFEVQLEELLNEV 120
            AST SASSRA  G+QRKH++SAFT PNGYQ R  HMH   DG + +EFE QL+EL NEV
Sbjct: 61  FASTGSASSRADLGSQRKHIASAFTVPNGYQSRPGHMHYRTDGTSTSEFEGQLDELFNEV 120

Query: 121 RMMTMSGRKNDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVL 180
           RM+ +SGRK+DAVELLQAN+EAVKEQMESGA+G EQAAVLDIVALGYITVGDLKFVAS+L
Sbjct: 121 RMLIVSGRKSDAVELLQANYEAVKEQMESGAIGIEQAAVLDIVALGYITVGDLKFVASIL 180

Query: 181 DILNKVVDSLTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLI 240
           DILN +VDSL D EPFLDSVLLHMGSMYSTLKK EKS SAYKRAIDIIEKKSG+DSSFLI
Sbjct: 181 DILNNIVDSLKDNEPFLDSVLLHMGSMYSTLKKLEKSMSAYKRAIDIIEKKSGKDSSFLI 240

Query: 241 TPILGMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKD 300
           TPILGMAKVLGT G+  KAVE Y+RAISILES RGFE+EDLVIPL SLGNL+LKEGKGKD
Sbjct: 241 TPILGMAKVLGTSGKTTKAVESYHRAISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKD 300

Query: 301 AETCFARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDL 360
           AETCFARIVNIYKKLYGEK+GKV MAM SLANAKCA+GEADEA+TLYRRAL+IIKDSN +
Sbjct: 301 AETCFARIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITLYRRALQIIKDSNYM 360

Query: 361 ALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASY 420
           ALDDS +EKMRIDLAELLH +GRG EGRELLEECLL+NE+ KGK+HPSSVKHLVNLAASY
Sbjct: 361 ALDDSEMEKMRIDLAELLHAVGRGKEGRELLEECLLINEKSKGKDHPSSVKHLVNLAASY 420

Query: 421 SRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLR 480
           SRSKNY EA RLLRIGLDIM+KAVG DDQSIT PMLNLAVTLYNLK+DDDAEQL LEVLR
Sbjct: 421 SRSKNYAEAERLLRIGLDIMVKAVGPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLR 480

Query: 481 IRENAFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTL 540
           IRENAFGKD LPVGEALDCLVSIQ+RLGKD++ELLKLLKRILRIQE+ FG ++KEVIDTL
Sbjct: 481 IRENAFGKDCLPVGEALDCLVSIQSRLGKDDTELLKLLKRILRIQEKAFGYEAKEVIDTL 540

Query: 541 KKIAFYMEKLGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           KKI FYM+KLGMKDEKF +QKRLS+LR KF+NQMQY
Sbjct: 541 KKIVFYMDKLGMKDEKFPVQKRLSLLRTKFKNQMQY 576

BLAST of PI0010853 vs. ExPASy TrEMBL
Match: A0A6J1K9C6 (nephrocystin-3 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 8.3e-249
Identity = 466/576 (80.90%), Postives = 513/576 (89.06%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCY---SHCICKRA-----SITCSSVCLQKPKCNIKLYAVPVGAFSCL 60
           MA FLLLSSPS+T +     C+C ++      ITCSSV LQK KCNIKLYAVPV AFSC 
Sbjct: 1   MAAFLLLSSPSFTYHRMSEICLCMQSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCR 60

Query: 61  SASTPSASSRAAFGAQRKHVSSAFTAPNGYQ-RSDHMH---DGNTLAEFEVQLEELLNEV 120
            AST SASSR   G+QRKH++SAFT PNGYQ R+ HMH   DGN+ +EFE QL+EL NEV
Sbjct: 61  FASTGSASSRDDLGSQRKHIASAFTVPNGYQSRAGHMHYRTDGNSASEFEGQLDELFNEV 120

Query: 121 RMMTMSGRKNDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVL 180
           RM+ +S RK+DAVELLQAN+EAVKEQMESGA G EQAAVLDIVALGYITVGDLKFVAS+L
Sbjct: 121 RMLIVSRRKSDAVELLQANYEAVKEQMESGACGIEQAAVLDIVALGYITVGDLKFVASIL 180

Query: 181 DILNKVVDSLTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLI 240
           DILN +VDSL D EPFLDSVLLHMGSMYSTLKK +KS SAYKRAIDIIEKKSG+DSSFLI
Sbjct: 181 DILNNIVDSLKDNEPFLDSVLLHMGSMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLI 240

Query: 241 TPILGMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKD 300
           TPILGMAKVLGT G+  KAVEFY+RAISILES RGFE+EDLVIPL SLGNL+LKEGKGKD
Sbjct: 241 TPILGMAKVLGTSGKTTKAVEFYHRAISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKD 300

Query: 301 AETCFARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDL 360
           AETCFARIVNIYKKLYGEK+GKV MAM SLANAKCA+GEADEA+TLYRRAL+IIKDSN +
Sbjct: 301 AETCFARIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITLYRRALQIIKDSNYM 360

Query: 361 ALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASY 420
           ALDDS +EKMRIDLAELLH +GRG EGRELLEECLL+NE+ KGK+HPSSVKHLVNLAASY
Sbjct: 361 ALDDSEMEKMRIDLAELLHAVGRGKEGRELLEECLLINEKSKGKDHPSSVKHLVNLAASY 420

Query: 421 SRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLR 480
           SRSKNY EA RLLRIGLDIM+KAVG DDQSIT PMLNLAVTLYNLK+DDDAEQL LEVLR
Sbjct: 421 SRSKNYAEAERLLRIGLDIMVKAVGPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLR 480

Query: 481 IRENAFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTL 540
           IRENAFGKD LPVGEALDCLVSIQ+RLGKDE+ELLKLLKRILRIQE+ FG ++KEVIDTL
Sbjct: 481 IRENAFGKDCLPVGEALDCLVSIQSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTL 540

Query: 541 KKIAFYMEKLGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           KKI FYM+KLG+KDEKF +QKRLS+LR+KF+NQMQY
Sbjct: 541 KKIVFYMDKLGLKDEKFPVQKRLSLLRMKFKNQMQY 576

BLAST of PI0010853 vs. ExPASy TrEMBL
Match: A0A6J1H150 (nephrocystin-3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 7.8e-247
Identity = 454/543 (83.61%), Postives = 494/543 (90.98%), Query Frame = 0

Query: 25  ITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSASSRAAFGAQRKHVSSAFTAPNGYQR 84
           ITCSSV LQK KCNIKLYAVPV AFSC  AST SASSRA  G+QRKH++SAFT PNGYQR
Sbjct: 42  ITCSSVSLQKQKCNIKLYAVPVRAFSCQFASTGSASSRADLGSQRKHIASAFTVPNGYQR 101

Query: 85  SDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRKNDAVELLQANFEAVKEQMESGAVG 144
             HMH   DG + +EFE QL+EL NEVRM+ +SGRK+DAVELLQAN+EAVKEQMESGA+G
Sbjct: 102 PGHMHYRTDGTSTSEFEGQLDELFNEVRMLIVSGRKSDAVELLQANYEAVKEQMESGAIG 161

Query: 145 FEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDSLTDGEPFLDSVLLHMGSMYSTLKK 204
            EQAAVLDIVALGYITVGDLKFVAS+LDILN +VDSL D EPFLDSVLLHMGSMYSTLKK
Sbjct: 162 IEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLHMGSMYSTLKK 221

Query: 205 FEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKVLGTIGRAGKAVEFYNRAISILESK 264
            EKS SAYKRAIDIIEKKSG+DSSFLITPILGMAKVLGT G+  KAVE Y+RAISILES 
Sbjct: 222 LEKSMSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVESYHRAISILEST 281

Query: 265 RGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIVNIYKKLYGEKNGKVVMAMCSLANA 324
           RGFE+EDLVIPL SLGNL+LKEGKGKDAETCFARIVNIYKKLYGEK+GKV MAM SLANA
Sbjct: 282 RGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVGMAMYSLANA 341

Query: 325 KCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEE 384
           KCA+GEADEA+TLYRRAL+IIKDSN +ALDDS +EKMRIDLAELLH +GRG EGRELLEE
Sbjct: 342 KCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGKEGRELLEE 401

Query: 385 CLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITV 444
           CLL+NE+ KGK+HPSSVKHLVNLAASYSRSKNY EA RLLRIGLDIM+KAVG DDQSIT 
Sbjct: 402 CLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVGPDDQSITN 461

Query: 445 PMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSLPVGEALDCLVSIQNRLGKDESE 504
           PMLNLAVTLYNLK+DDDAEQL LEVLRIRENAFGKD LPVGEALDCLVSIQ+RLGKD++E
Sbjct: 462 PMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQSRLGKDDTE 521

Query: 505 LLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEKLGMKDEKFLLQKRLSMLRIKFRNQ 564
           LLKLLKRILRIQE+ FG ++KEVIDTLKKI FYM+KLGMKDEKF +QKRLS+LR KF+NQ
Sbjct: 522 LLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGMKDEKFPVQKRLSLLRTKFKNQ 581

BLAST of PI0010853 vs. NCBI nr
Match: XP_008437615.1 (PREDICTED: nephrocystin-3 [Cucumis melo] >XP_016898886.1 PREDICTED: nephrocystin-3 [Cucumis melo])

HSP 1 Score: 986.5 bits (2549), Expect = 9.6e-284
Identity = 523/567 (92.24%), Postives = 541/567 (95.41%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSAS 60
           MAT LLLSSPSYTCYSHCICKR SITCSSVCLQKP CNIKLYA P GAFSCLSASTPSAS
Sbjct: 1   MATSLLLSSPSYTCYSHCICKRPSITCSSVCLQKPLCNIKLYAGPFGAFSCLSASTPSAS 60

Query: 61  SRAAFGAQRKHVSSAFTAPNGYQRSDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRK 120
           SRAAFGAQRKHV SAFTAP GYQRSDHMH   D +T+AE EVQLEELLNEVRMMTMSGRK
Sbjct: 61  SRAAFGAQRKHVFSAFTAPIGYQRSDHMHYRTDSDTVAELEVQLEELLNEVRMMTMSGRK 120

Query: 121 NDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDS 180
           NDAVELLQAN+E VKEQMESGAVG EQAA+LDIVALGY+TVGDLKFVASVLDILNKVVDS
Sbjct: 121 NDAVELLQANYEVVKEQMESGAVGIEQAALLDIVALGYLTVGDLKFVASVLDILNKVVDS 180

Query: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKV 240
           LTDGEPFLDSVLLHMGSMYSTLKKFEKS SAYKRAIDI+EKK GEDSSFLITPILGMAKV
Sbjct: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSVSAYKRAIDIMEKKFGEDSSFLITPILGMAKV 240

Query: 241 LGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIV 300
           LGTIGRAGKAVE YNRAIS+LES+RGFENEDLVIPL SLGNLMLKEGKGKDAETCFARIV
Sbjct: 241 LGTIGRAGKAVECYNRAISLLESRRGFENEDLVIPLFSLGNLMLKEGKGKDAETCFARIV 300

Query: 301 NIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEK 360
           NIYKKLYGEKNGKV MAM SLANAK A+GEADEAVTLYRRALEIIKDSND+A+DDST EK
Sbjct: 301 NIYKKLYGEKNGKVGMAMYSLANAKYARGEADEAVTLYRRALEIIKDSNDMAVDDSTTEK 360

Query: 361 MRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420
           MRIDLAELLHVLGRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSKNYVEA
Sbjct: 361 MRIDLAELLHVLGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420

Query: 421 ARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKD 480
            RLLRIGLDIMIKAVGSDDQSITVPML+LAVTLYNLKQDD+AEQL LEVLRIRENAFGKD
Sbjct: 421 ERLLRIGLDIMIKAVGSDDQSITVPMLDLAVTLYNLKQDDEAEQLALEVLRIRENAFGKD 480

Query: 481 SLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEK 540
           SLPVGEALDCLVSIQ+RLGKDESELLKLLKRILRIQEREFGDD +EVIDTLKKIAF MEK
Sbjct: 481 SLPVGEALDCLVSIQSRLGKDESELLKLLKRILRIQEREFGDDGEEVIDTLKKIAFCMEK 540

Query: 541 LGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           LGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 LGMKDEKFLLQKRLSMLRMKFKNQMQY 567

BLAST of PI0010853 vs. NCBI nr
Match: XP_011654607.1 (nephrocystin-3 [Cucumis sativus] >XP_011654608.1 nephrocystin-3 [Cucumis sativus] >KGN49566.2 hypothetical protein Csa_000372 [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 1.1e-276
Identity = 518/567 (91.36%), Postives = 538/567 (94.89%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSAS 60
           MATFLLLSSPS   YSHCICKRASITCSSVCLQ  KCNIKLYAVPVGAFSCLSASTPSAS
Sbjct: 1   MATFLLLSSPS---YSHCICKRASITCSSVCLQ--KCNIKLYAVPVGAFSCLSASTPSAS 60

Query: 61  SRAAFGAQRKHVSSAFTAPNGYQRSDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRK 120
           SRAAFGA+RKHVSSAFT P GYQRSDHMH   D NT+AEFEVQLEELLNEVR +TMSGRK
Sbjct: 61  SRAAFGAERKHVSSAFT-PIGYQRSDHMHYRTDCNTVAEFEVQLEELLNEVRTLTMSGRK 120

Query: 121 NDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDS 180
           +DAVELLQAN+EAVKEQMESGAVG EQAAVLDIVALGY+TVGDLKFVASVLDILNKVVDS
Sbjct: 121 SDAVELLQANYEAVKEQMESGAVGIEQAAVLDIVALGYLTVGDLKFVASVLDILNKVVDS 180

Query: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKV 240
           LTDGEPFLDSVLLHMGSMYSTLKKFEKS S YKRAIDI+EKK GEDSSFLITPILGMAKV
Sbjct: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSISVYKRAIDIMEKKFGEDSSFLITPILGMAKV 240

Query: 241 LGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIV 300
           LGTIGRAGKAVE YNRAIS+LES RGFENEDLVIPL SLGNLMLKEGKGKDAETCFARIV
Sbjct: 241 LGTIGRAGKAVECYNRAISLLESSRGFENEDLVIPLISLGNLMLKEGKGKDAETCFARIV 300

Query: 301 NIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEK 360
           NIY+KLYGEKNGKV MAM SLANAKCA+GEADEAVTL RRALEIIKDSND+ALDDSTIEK
Sbjct: 301 NIYEKLYGEKNGKVGMAMYSLANAKCARGEADEAVTLCRRALEIIKDSNDIALDDSTIEK 360

Query: 361 MRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420
           MRIDLAELLHVLGR NEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSKNYVEA
Sbjct: 361 MRIDLAELLHVLGRANEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420

Query: 421 ARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKD 480
            RLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQD+DAEQL LEVLRIRENAFGKD
Sbjct: 421 ERLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDNDAEQLALEVLRIRENAFGKD 480

Query: 481 SLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEK 540
           SLPVGEALDCLVSIQ+RLGKDESELLKLLKRIL IQE+EFG+D KEVIDTLKKI F M+K
Sbjct: 481 SLPVGEALDCLVSIQSRLGKDESELLKLLKRILTIQEKEFGEDGKEVIDTLKKIVFCMDK 540

Query: 541 LGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           LGMKDEKFLLQKRLSMLR+KF+NQM+Y
Sbjct: 541 LGMKDEKFLLQKRLSMLRMKFKNQMRY 561

BLAST of PI0010853 vs. NCBI nr
Match: XP_038875999.1 (nephrocystin-3 isoform X1 [Benincasa hispida] >XP_038876001.1 nephrocystin-3 isoform X1 [Benincasa hispida] >XP_038876002.1 nephrocystin-3 isoform X1 [Benincasa hispida])

HSP 1 Score: 918.3 bits (2372), Expect = 3.2e-263
Identity = 494/572 (86.36%), Postives = 518/572 (90.56%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHC-IC-------KRASITCSSVCLQKPKCNIKLYAVPVGAFSCL 60
           MATFLLLS PS+TC     IC        +  ITCSSVCLQK KC+IKLYAVPV AFSC 
Sbjct: 1   MATFLLLSPPSFTCNRMSEICLSVQSLQLQKGITCSSVCLQKQKCDIKLYAVPVRAFSCC 60

Query: 61  SASTPSASSRAAFGAQRKHVSSAFTAPNGYQRSDHMHDGNTLAEFEVQLEELLNEVRMMT 120
            ASTPSASSRA  G QRKH+ SAFTAPNGYQR       N + EFEVQLEEL NEVRMMT
Sbjct: 61  FASTPSASSRADSGRQRKHIPSAFTAPNGYQR-------NRMTEFEVQLEELFNEVRMMT 120

Query: 121 MSGRKNDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILN 180
           MSGRKNDAVELLQAN+EAVKEQMESGA+G EQAAVLDIVALGYITVGDLKFVASVLDILN
Sbjct: 121 MSGRKNDAVELLQANYEAVKEQMESGAIGIEQAAVLDIVALGYITVGDLKFVASVLDILN 180

Query: 181 KVVDSLTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPIL 240
           KVVDSL D EPFLDSVLLHMGSMYSTLKKFEKS SAYKR+IDIIEKK+GEDSSFLITPIL
Sbjct: 181 KVVDSLKDSEPFLDSVLLHMGSMYSTLKKFEKSISAYKRSIDIIEKKNGEDSSFLITPIL 240

Query: 241 GMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETC 300
           GMAKV GTIGR GKAVEFY+RAIS+LES RGFENEDLVIPLS LGNLMLKEGKGKDAETC
Sbjct: 241 GMAKVFGTIGRTGKAVEFYHRAISLLESSRGFENEDLVIPLSGLGNLMLKEGKGKDAETC 300

Query: 301 FARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDD 360
           FARIVNIYKKLYGEK+GKV MAM SLANAKCA+GEADEAVTLYRRAL+IIKDSND+ALD 
Sbjct: 301 FARIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAVTLYRRALQIIKDSNDMALDA 360

Query: 361 STIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSK 420
           S +EKMRIDLAELLHV+GRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSK
Sbjct: 361 SVMEKMRIDLAELLHVVGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSK 420

Query: 421 NYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIREN 480
           NY EA RLLRIGLDIMIKAVG DDQSITVPMLNLAVTLYNLK+DDDAEQL LEVLRIREN
Sbjct: 421 NYAEAERLLRIGLDIMIKAVGPDDQSITVPMLNLAVTLYNLKRDDDAEQLALEVLRIREN 480

Query: 481 AFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIA 540
           AFGKDSLPVGEALDCLVSIQ+RLGKDE+ELLKLL+RIL IQEREFG D KEVIDTLKKI 
Sbjct: 481 AFGKDSLPVGEALDCLVSIQSRLGKDENELLKLLERILIIQEREFGHDGKEVIDTLKKIV 540

Query: 541 FYMEKLGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           FYMEKLGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 FYMEKLGMKDEKFLLQKRLSMLRMKFKNQMQY 565

BLAST of PI0010853 vs. NCBI nr
Match: XP_038876003.1 (nephrocystin-3 isoform X2 [Benincasa hispida])

HSP 1 Score: 915.6 bits (2365), Expect = 2.1e-262
Identity = 493/572 (86.19%), Postives = 517/572 (90.38%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHC-IC-------KRASITCSSVCLQKPKCNIKLYAVPVGAFSCL 60
           MATFLLLS PS+TC     IC        +  ITCSSVCLQK KC+IKLYAVPV AFSC 
Sbjct: 1   MATFLLLSPPSFTCNRMSEICLSVQSLQLQKGITCSSVCLQKQKCDIKLYAVPVRAFSCC 60

Query: 61  SASTPSASSRAAFGAQRKHVSSAFTAPNGYQRSDHMHDGNTLAEFEVQLEELLNEVRMMT 120
            ASTPSASSRA  G QRKH+ SAFTAPNGYQR         + EFEVQLEEL NEVRMMT
Sbjct: 61  FASTPSASSRADSGRQRKHIPSAFTAPNGYQR---------MTEFEVQLEELFNEVRMMT 120

Query: 121 MSGRKNDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILN 180
           MSGRKNDAVELLQAN+EAVKEQMESGA+G EQAAVLDIVALGYITVGDLKFVASVLDILN
Sbjct: 121 MSGRKNDAVELLQANYEAVKEQMESGAIGIEQAAVLDIVALGYITVGDLKFVASVLDILN 180

Query: 181 KVVDSLTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPIL 240
           KVVDSL D EPFLDSVLLHMGSMYSTLKKFEKS SAYKR+IDIIEKK+GEDSSFLITPIL
Sbjct: 181 KVVDSLKDSEPFLDSVLLHMGSMYSTLKKFEKSISAYKRSIDIIEKKNGEDSSFLITPIL 240

Query: 241 GMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETC 300
           GMAKV GTIGR GKAVEFY+RAIS+LES RGFENEDLVIPLS LGNLMLKEGKGKDAETC
Sbjct: 241 GMAKVFGTIGRTGKAVEFYHRAISLLESSRGFENEDLVIPLSGLGNLMLKEGKGKDAETC 300

Query: 301 FARIVNIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDD 360
           FARIVNIYKKLYGEK+GKV MAM SLANAKCA+GEADEAVTLYRRAL+IIKDSND+ALD 
Sbjct: 301 FARIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAVTLYRRALQIIKDSNDMALDA 360

Query: 361 STIEKMRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSK 420
           S +EKMRIDLAELLHV+GRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSK
Sbjct: 361 SVMEKMRIDLAELLHVVGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSK 420

Query: 421 NYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIREN 480
           NY EA RLLRIGLDIMIKAVG DDQSITVPMLNLAVTLYNLK+DDDAEQL LEVLRIREN
Sbjct: 421 NYAEAERLLRIGLDIMIKAVGPDDQSITVPMLNLAVTLYNLKRDDDAEQLALEVLRIREN 480

Query: 481 AFGKDSLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIA 540
           AFGKDSLPVGEALDCLVSIQ+RLGKDE+ELLKLL+RIL IQEREFG D KEVIDTLKKI 
Sbjct: 481 AFGKDSLPVGEALDCLVSIQSRLGKDENELLKLLERILIIQEREFGHDGKEVIDTLKKIV 540

Query: 541 FYMEKLGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           FYMEKLGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 FYMEKLGMKDEKFLLQKRLSMLRMKFKNQMQY 563

BLAST of PI0010853 vs. NCBI nr
Match: KAA0042543.1 (nephrocystin-3 [Cucumis melo var. makuwa] >TYK05947.1 nephrocystin-3 [Cucumis melo var. makuwa])

HSP 1 Score: 909.8 bits (2350), Expect = 1.1e-260
Identity = 492/567 (86.77%), Postives = 509/567 (89.77%), Query Frame = 0

Query: 1   MATFLLLSSPSYTCYSHCICKRASITCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSAS 60
           MAT LLLSSPSYTCYSHCICKR SITCSSVCLQKP CNIKLYA P GAFSCLSASTPSAS
Sbjct: 1   MATSLLLSSPSYTCYSHCICKRPSITCSSVCLQKPLCNIKLYAGPFGAFSCLSASTPSAS 60

Query: 61  SRAAFGAQRKHVSSAFTAPNGYQRSDHMH---DGNTLAEFEVQLEELLNEVRMMTMSGRK 120
           SRAAFGAQRKHV SAFTAP GYQRSDHMH   D +T+AE EVQLEELLNEVRMMTMSGRK
Sbjct: 61  SRAAFGAQRKHVFSAFTAPIGYQRSDHMHYRTDSDTVAELEVQLEELLNEVRMMTMSGRK 120

Query: 121 NDAVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDS 180
           NDAVELLQAN+E VKEQMESGAVG EQAA+LDIVALGY+TVGDLKFVASVLDILNKVVDS
Sbjct: 121 NDAVELLQANYEVVKEQMESGAVGIEQAALLDIVALGYLTVGDLKFVASVLDILNKVVDS 180

Query: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKV 240
           LTDGEPFLDSVLLHMGSMYSTLKKFEKS SAYKRAIDI+EKK GEDSSFLITPILGMAKV
Sbjct: 181 LTDGEPFLDSVLLHMGSMYSTLKKFEKSVSAYKRAIDIMEKKFGEDSSFLITPILGMAKV 240

Query: 241 LGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIV 300
           LGTIGRAGKAVE YNRAIS+LES+RGFENEDLVIPL SLGNLMLKEGKGKDAET      
Sbjct: 241 LGTIGRAGKAVECYNRAISLLESRRGFENEDLVIPLFSLGNLMLKEGKGKDAET------ 300

Query: 301 NIYKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEK 360
                                        EADEAVTLYRRALEIIKDSND+A+DDST EK
Sbjct: 301 ----------------------------WEADEAVTLYRRALEIIKDSNDMAVDDSTTEK 360

Query: 361 MRIDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420
           MRIDLAELLHVLGRGNEGRELLEECLL+NERLKGKEHPSSVKHLVNLAASYSRSKNYVEA
Sbjct: 361 MRIDLAELLHVLGRGNEGRELLEECLLINERLKGKEHPSSVKHLVNLAASYSRSKNYVEA 420

Query: 421 ARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKD 480
            RLLRIGLDIMIKAVGSDDQSITVPML+LAVTLYNLKQDD+AEQL LEVLRIRENAFGKD
Sbjct: 421 ERLLRIGLDIMIKAVGSDDQSITVPMLDLAVTLYNLKQDDEAEQLALEVLRIRENAFGKD 480

Query: 481 SLPVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEK 540
           SLPVGEALDCLVSIQ+RLGKDESELLKLLKRILRIQEREFGDD +EVIDTLKKIAF MEK
Sbjct: 481 SLPVGEALDCLVSIQSRLGKDESELLKLLKRILRIQEREFGDDGEEVIDTLKKIAFCMEK 533

Query: 541 LGMKDEKFLLQKRLSMLRIKFRNQMQY 565
           LGMKDEKFLLQKRLSMLR+KF+NQMQY
Sbjct: 541 LGMKDEKFLLQKRLSMLRMKFKNQMQY 533

BLAST of PI0010853 vs. TAIR 10
Match: AT5G53080.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 586.3 bits (1510), Expect = 2.7e-167
Identity = 316/565 (55.93%), Postives = 420/565 (74.34%), Query Frame = 0

Query: 5   LLLSSPSYTCYSHCICKRASI-----TCSSVCLQKPKCNIKLYAVPVGAFSCLSASTPSA 64
           LL S+ S T +++    ++S+     T   VCL+  K   KLY +P   F      + S+
Sbjct: 7   LLSSTTSLTTWANQSSSQSSLSPRYSTWQCVCLRNQKRKPKLYLIPARHFLSTPIDSVSS 66

Query: 65  SSRAAFGAQRKHVSSAFTAPNGYQRSDHMHDGNTLAEFEVQLEELLNEVRMMTMSGRKND 124
           SS  A       VS         QRS   ++   + EFE++L+EL NEV+ M   G+++D
Sbjct: 67  SSITASRYATSGVSEV-------QRSTSSNNVTEMEEFEMELQELFNEVKSMVKIGKESD 126

Query: 125 AVELLQANFEAVKEQMESGAVGFEQAAVLDIVALGYITVGDLKFVASVLDILNKVVDSLT 184
           A++LL+AN+ AVKE+++SG  G EQAAVLDI+ALGY+ VGDLK V ++LD++NK+VD+L 
Sbjct: 127 AMDLLRANYVAVKEELDSGLKGIEQAAVLDIIALGYMAVGDLKPVPALLDMINKIVDNLK 186

Query: 185 DGEPFLDSVLLHMGSMYSTLKKFEKSKSAYKRAIDIIEKKSGEDSSFLITPILGMAKVLG 244
           D EP LDSVL+H+GSMYS + KFE +   ++RAI I+E + G+ ++ L+TP+LGMAK   
Sbjct: 187 DSEPLLDSVLMHVGSMYSVIGKFENAILVHQRAIRILENRYGKCNTLLVTPLLGMAKSFA 246

Query: 245 TIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSSLGNLMLKEGKGKDAETCFARIVNI 304
           + G+A KA+  Y R ++ILE  RG E+EDLV+PL SLG L+LKEGK  +AE  F  IVNI
Sbjct: 247 SDGKATKAIGVYERTLTILERNRGSESEDLVVPLFSLGKLLLKEGKAAEAEIPFTSIVNI 306

Query: 305 YKKLYGEKNGKVVMAMCSLANAKCAKGEADEAVTLYRRALEIIKDSNDLALDDSTIEKMR 364
           YKK+YGE++G+V MAMCSLANAKC+KG+A+EAV +YR AL IIKDSN + +D+S +E MR
Sbjct: 307 YKKIYGERDGRVGMAMCSLANAKCSKGDANEAVDIYRNALRIIKDSNYMTIDNSILENMR 366

Query: 365 IDLAELLHVLGRGNEGRELLEECLLVNERLKGKEHPSSVKHLVNLAASYSRSKNYVEAAR 424
           IDLAELLH +GRG+EGRELLEECLL+NER KGK HPS   HL+NLAASYSRSKNYVEA R
Sbjct: 367 IDLAELLHFVGRGDEGRELLEECLLINERFKGKNHPSMATHLINLAASYSRSKNYVEAER 426

Query: 425 LLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNLKQDDDAEQLVLEVLRIRENAFGKDSL 484
           LLR  L+IM  +VGS+ QSIT PMLNLAVTL  L +D++AEQ+ L+VLRIRE AFG+DSL
Sbjct: 427 LLRTCLNIMEVSVGSEGQSITFPMLNLAVTLSQLNRDEEAEQIALKVLRIREKAFGEDSL 486

Query: 485 PVGEALDCLVSIQNRLGKDESELLKLLKRILRIQEREFGDDSKEVIDTLKKIAFYMEKLG 544
           PVGEALDCLVSIQ RLG+D+ E+L LLKR++ IQE+EFG  ++E+I TL+KI  ++EKL 
Sbjct: 487 PVGEALDCLVSIQARLGRDDGEILGLLKRVMMIQEKEFGPSAQELIVTLQKIIHFLEKLE 546

Query: 545 MKDEKFLLQKRLSMLRIKFRNQMQY 565
           MKD+KF  ++RL++LR +++  + Y
Sbjct: 547 MKDDKFKFRRRLALLRERYKQSLSY 564

BLAST of PI0010853 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-05
Identity = 91/452 (20.13%), Postives = 175/452 (38.72%), Query Frame = 0

Query: 104 LLNEVRMMTMSGRK-NDAVELLQANFEAVKEQMESG----AVGFEQAAVLDIVALGYITV 163
           LL + R +  SG   N A++L      AVK   + G     +G      L I+A  Y  +
Sbjct: 157 LLKQARELVSSGENLNKALDLA---LRAVKVFEKCGEGEKQLGLNLVMSLHILAAIYAGL 216

Query: 164 GDLKFVASVLDILNKVVDSLTDGEPFLDSVL---LHMGSMYSTLKKFEKSKSAYKRAIDI 223
           G       VL+  +  +  + DGE    +     + +G MY  + + E S   Y   ++I
Sbjct: 217 GRYNDAVPVLE-RSIEIPMIEDGEDHALAKFAGCMQLGDMYGLMGQVENSIMLYTAGLEI 276

Query: 224 IEKKSGEDSSFLITPILGMAKVLGTIGRAGKAVEFYNRAISILESKRGFENEDLVIPLSS 283
             +  GE  + +      +A+      +  +A      A+ I +         +      
Sbjct: 277 QRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAATASIEEAADR 336

Query: 284 LGNLMLKEGKGKDAETCFARIVNIYKKLYGEKNGKVVMAM-CSLANAKCAKGEADEAVTL 343
               ++ + KG D E      V     +  + + + V A+ CS+ +A  +    DEA+  
Sbjct: 337 KLMGLICDAKG-DYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLARFDEAIFA 396

Query: 344 YRRALEIIKDSNDLALDDSTIEKMRIDLAELLHVLGRGNEGRELLEECLLVN-ERLKGKE 403
           Y++AL + K         S++  + + LA+L + +G+  + +   E  L +  +   G  
Sbjct: 397 YQKALAVFKQGK--GETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLKPTPGTP 456

Query: 404 HPSSVKHLVNLAASYSRSKNYVEAARLLRIGLDIMIKAVGSDDQSITVPMLNLAVTLYNL 463
                   + ++A Y       +A +LLR  L I   A G  + +I      + V  Y +
Sbjct: 457 MEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQN-TIAGIEAQMGVVTYMM 516

Query: 464 KQDDDAEQLVLEVLRIRENAFGKDSLPVGEALD--CLVSIQNRLGKDESELLKLLKRILR 523
               ++  +    +    N+  K +   G AL+   L  +Q     + ++L +  K IL 
Sbjct: 517 GNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEAKTIL- 576

Query: 524 IQEREFGDDSKEVIDTLKKIAFYMEKLGMKDE 544
             E+E G    + +     +A   + +G  D+
Sbjct: 577 --EKECGPYHPDTLAVYSNLAGTYDAMGRLDD 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CI653.5e-1826.30Nephrocystin-3 OS=Danio rerio OX=7955 GN=nphp3 PE=3 SV=1[more]
Q078662.4e-1126.96Kinesin light chain 1 OS=Homo sapiens OX=9606 GN=KLC1 PE=1 SV=2[more]
Q5R5812.4e-1126.96Kinesin light chain 1 OS=Pongo abelii OX=9601 GN=KLC1 PE=2 SV=3[more]
P468254.1e-1126.96Kinesin light chain OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1[more]
P372855.4e-1126.47Kinesin light chain 1 OS=Rattus norvegicus OX=10116 GN=Klc1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3AV294.6e-28492.24nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103482972 PE=4 SV=1[more]
A0A5D3C2905.5e-26186.77Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00400... [more]
A0A6J1H2E01.7e-24981.08nephrocystin-3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=... [more]
A0A6J1K9C68.3e-24980.90nephrocystin-3 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1[more]
A0A6J1H1507.8e-24783.61nephrocystin-3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_008437615.19.6e-28492.24PREDICTED: nephrocystin-3 [Cucumis melo] >XP_016898886.1 PREDICTED: nephrocystin... [more]
XP_011654607.11.1e-27691.36nephrocystin-3 [Cucumis sativus] >XP_011654608.1 nephrocystin-3 [Cucumis sativus... [more]
XP_038875999.13.2e-26386.36nephrocystin-3 isoform X1 [Benincasa hispida] >XP_038876001.1 nephrocystin-3 iso... [more]
XP_038876003.12.1e-26286.19nephrocystin-3 isoform X2 [Benincasa hispida][more]
KAA0042543.11.1e-26086.77nephrocystin-3 [Cucumis melo var. makuwa] >TYK05947.1 nephrocystin-3 [Cucumis me... [more]
Match NameE-valueIdentityDescription
AT5G53080.12.7e-16755.93Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.11.5e-0520.13Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..219
NoneNo IPR availableCOILSCoilCoilcoord: 94..114
NoneNo IPR availablePFAMPF13374TPR_10coord: 443..478
e-value: 4.5E-6
score: 26.3
NoneNo IPR availablePFAMPF13424TPR_12coord: 188..260
e-value: 5.2E-9
score: 36.3
coord: 273..343
e-value: 3.4E-10
score: 40.1
coord: 358..427
e-value: 1.1E-9
score: 38.4
NoneNo IPR availablePANTHERPTHR45641TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)coord: 101..549
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 356..389
e-value: 410.0
score: 0.8
coord: 440..473
e-value: 53.0
score: 8.6
coord: 312..345
e-value: 0.12
score: 21.5
coord: 270..303
e-value: 130.0
score: 5.2
coord: 186..219
e-value: 10.0
score: 15.0
coord: 228..261
e-value: 100.0
score: 6.2
coord: 398..431
e-value: 140.0
score: 4.8
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 186..219
score: 8.6439
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 111..348
e-value: 1.0E-27
score: 99.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 352..559
e-value: 3.0E-29
score: 104.0
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 188..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010853.2PI0010853.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0031425 chloroplast RNA processing
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding