PI0010392 (gene) Melon (PI 482460) v1

Overview
NamePI0010392
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionpre-mRNA-splicing factor CWC25 homolog
Locationchr07: 3482055 .. 3484293 (-)
RNA-Seq ExpressionPI0010392
SyntenyPI0010392
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAAACCACACCGCCGGTTGCTGAAAATCCATCATCCGATCCCTAATCTGAAGTTCTGTTACAGACATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAGCAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCAGGAGGAGAAAGAACGCCAAGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTTCGTATGTTTCATCTTCTATCTTTTTATTTCATTCGTGTTCTGTGAATTTCAAAATCTGATTCTATTTCTATGAGGATTGAAACCGTTGCGTAGATTAGGGTTAGTGCCAAGGCGATGGCTAGGGTTTAATTAGTTCGATTGATTTGACCAATATGCAGGAAGCAGGAAAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTCGGGAAAGCTAGCAGTTCCGATGGGTTTAAGAGCCTTGAAACCCTACCTAGTTCATCTACTGCCGCGGCTGCCACTGAACCATCTAGTTCCAAGGTACTGTTGCGTTTTTTCGTTGATTTGTGTGCTTTGGCTATCTCTTTGTATTGCTCAATTATGATGTTTGTTGGATTCTTGTGTTGTTTATGCAGGAAACCGCTGTCCCTGGAGCTCTGTTTGAGGATAAACCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAGCAAGCTCTTGCTCGCGTAAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTAAGTGTTTTTTAATCTCCTTATATGGCTTTAACCCCATTAAACTTTACAAGTTTTAATGTTATAACATGGCCTGTGGAAATTTTGTGGAAACATCTTTCTTCCATTCATTATTTTGTAGAGAGATTTTCTGAACTTCTCTGTTAGTTTGTTCGTGGACACTTTACTTTGAGGTTGAGAAATCTGGTGATCTATCTAAGTTCTAAGTTATCCTTTTGATAGGAGCTAAAGATTAATCTTTGATCAGAAAATTTCTTTCTATATGTCTTCATCACTAGTCACTTGTAGCAAAAGTTGAAGCTTGTGTTGAAGAATTTCTATGACAAAACTGCCATATTAAAGAATATTCTTGTTGAGTTGTGATTGAATCTCTCTTTAATTCACTATCGTATTATGTTGAAAGCTCTCTCTTGTTTGATTTGAAGAATAGATATGAGGGTTTGTTTTTTTAATTATTTCCTCAAATATAAATCATAGGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTGTGATCCTGAAGATGCCAGCCCTGAGAGGCAGAGAAGGAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATTCTAAGAACGATAGGGATCGCGATAGAGGCTCAAAATATGCTGCACGAACGAGCTATGATCAGTCAGATCGAAAAACATCCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGACAGAGATTCTTATGCTAACAATGACAAGGACAGCAGAAGAGTGGGGGAACCGAGATATTATGAATCCAATGCTTCTGAAACTCCTCACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCTGAGTTACATGAAGAGCAACGATGGAAGCGTTTGAAGAAGGCAGCTGAGGATGATGCACTGGAAGCCAAACAAAATGCAGCACCCTCGAGCCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTGAAATTGAATCCAATGCTTTTAGACGGTAATGAACATCACCTTGTATACTTCTGCTTGTGGTTATTGTATAATTTTCCTTTTGAAGAATTGCTTCTTCATTTATCAATGTGATCTGATCTGATTTGGATTATGTTGACGATTACATTCATATATGGTTCGTTTTAATTAAATCGTTGTATTTTCTAATGATATTGATATATATTATCAACTTATCTAA

mRNA sequence

CACAAAACCACACCGCCGGTTGCTGAAAATCCATCATCCGATCCCTAATCTGAAGTTCTGTTACAGACATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAGCAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCAGGAGGAGAAAGAACGCCAAGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTTCGAAGCAGGAAAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTCGGGAAAGCTAGCAGTTCCGATGGGTTTAAGAGCCTTGAAACCCTACCTAGTTCATCTACTGCCGCGGCTGCCACTGAACCATCTAGTTCCAAGGAAACCGCTGTCCCTGGAGCTCTGTTTGAGGATAAACCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAGCAAGCTCTTGCTCGCGTAAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTGTGATCCTGAAGATGCCAGCCCTGAGAGGCAGAGAAGGAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATTCTAAGAACGATAGGGATCGCGATAGAGGCTCAAAATATGCTGCACGAACGAGCTATGATCAGTCAGATCGAAAAACATCCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGACAGAGATTCTTATGCTAACAATGACAAGGACAGCAGAAGAGTGGGGGAACCGAGATATTATGAATCCAATGCTTCTGAAACTCCTCACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCTGAGTTACATGAAGAGCAACGATGGAAGCGTTTGAAGAAGGCAGCTGAGGATGATGCACTGGAAGCCAAACAAAATGCAGCACCCTCGAGCCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTGAAATTGAATCCAATGCTTTTAGACGGTAATGAACATCACCTTGTATACTTCTGCTTGTGGTTATTGTATAATTTTCCTTTTGAAGAATTGCTTCTTCATTTATCAATGTGATCTGATCTGATTTGGATTATGTTGACGATTACATTCATATATGGTTCGTTTTAATTAAATCGTTGTATTTTCTAATGATATTGATATATATTATCAACTTATCTAA

Coding sequence (CDS)

ATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAGCAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCAGGAGGAGAAAGAACGCCAAGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTTCGAAGCAGGAAAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTCGGGAAAGCTAGCAGTTCCGATGGGTTTAAGAGCCTTGAAACCCTACCTAGTTCATCTACTGCCGCGGCTGCCACTGAACCATCTAGTTCCAAGGAAACCGCTGTCCCTGGAGCTCTGTTTGAGGATAAACCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAGCAAGCTCTTGCTCGCGTAAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTTGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTGTGATCCTGAAGATGCCAGCCCTGAGAGGCAGAGAAGGAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGATTCTAAGAACGATAGGGATCGCGATAGAGGCTCAAAATATGCTGCACGAACGAGCTATGATCAGTCAGATCGAAAAACATCCAAAAGCAATCCTCATGATTCTGCAGCAGATAGACATCATGACAGATCAAAGAGAGACAGAGATTCTTATGCTAACAATGACAAGGACAGCAGAAGAGTGGGGGAACCGAGATATTATGAATCCAATGCTTCTGAAACTCCTCACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCTGAGTTACATGAAGAGCAACGATGGAAGCGTTTGAAGAAGGCAGCTGAGGATGATGCACTGGAAGCCAAACAAAATGCAGCACCCTCGAGCCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTGAAATTGAATCCAATGCTTTTAGACGGTAA

Protein sequence

MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFEDKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKNDRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR
Homology
BLAST of PI0010392 vs. ExPASy Swiss-Prot
Match: Q9DBF7 (Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 2.8e-17
Identity = 130/437 (29.75%), Postives = 213/437 (48.74%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLV-SKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL+++++EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKETA-VPGAL 127
           +LD++Y+   G+       +G+      F+ +E            E   S ET  +PG++
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKME----------EREAGCSSETGLLPGSI 128

Query: 128 FEDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKN 187
           F   P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ +++   K + 
Sbjct: 129 F--APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEK 188

Query: 188 PDDKRERKKHRHSKSKRHKDSSPERDCDPEDASPERQRRK--HDHDKSSRHDGHSHSEDR 247
              K ++KKHR  K   H+ SS       ++ S  R ++K  +     S+  G+      
Sbjct: 189 KKKKEKKKKHRKHK---HRSSSSGGSSSEDEQSQARSQKKMANSFPVLSKVPGYGL---- 248

Query: 248 RSKADSKNDRDRDRG--SKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANN 307
                   D DR+RG       + +   + R  S S P  ++     +   RDR S + +
Sbjct: 249 -----QVRDSDRNRGLQGSLGEQRAIKNNSRSRSSSPPRHASKKSTKEERPRDRRSRSPS 308

Query: 308 DKD-SRRVGEPRYYESNASE----TPHESRHRHRRP---TTKLSEEERAARLREMQQDAE 367
            +  S R  +P   + N  E    +P +  ++ R     T KLS EE   + +EM ++A+
Sbjct: 309 RRSRSPRPSKPHTSKVNRKERDSPSPKKEAYQRRHASGYTRKLSAEELERKRQEMMENAK 368

Query: 368 LHEEQRWKRLKKAAEDDALEAKQNAAPS-SRNFLDMAQKRMYGAEKGGSSTIEESIRRRT 418
             EE+R   LK+ A +D  E +     S S  FL   +      E   +S++E+ ++R  
Sbjct: 369 WREEERLNTLKRHAREDEREHRLERLDSRSGKFLHRMK-----LESASTSSLEDRVKRNI 416

BLAST of PI0010392 vs. ExPASy Swiss-Prot
Match: Q9NXE8 (Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 6.3e-17
Identity = 132/442 (29.86%), Postives = 219/442 (49.55%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLV-SKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL+++++EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKETA-VPGAL 127
           +LD++Y+   G+       +G+      F+ +E            E   S ET  +PG++
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKME----------EKEAGCSSETGLLPGSI 128

Query: 128 FEDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKN 187
           F   P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ +++   K + 
Sbjct: 129 F--APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEK 188

Query: 188 PDDKRERKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKS--SRHDGHS---HS 247
              K ++KKH   K  +H+ SS +R    ++ S  R ++K  +     S+  G+     +
Sbjct: 189 KKKKEKKKKH---KKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRN 248

Query: 248 EDRRSKADSKNDRDRDRG----SKYAARTSYDQSDRKTSKSNPHDSAA-DRHH----DRS 307
            DR          ++ RG    +   +R+S     R  SK +  ++ + DR       RS
Sbjct: 249 SDRNQGLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRS 308

Query: 308 KRDRDSYANNDKDSRR-VGEPRYYESNASETPHESRHRHRRP---TTKLSEEERAARLRE 367
           +  R S  +N K +RR  G+ R      S +P +  ++ R     T KLS EE   + +E
Sbjct: 309 RSPRPSKLHNSKVNRRETGQTR------SPSPKKEVYQRRHAPGYTRKLSAEELERKRQE 368

Query: 368 MQQDAELHEEQRWKRLKKAAEDDALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEES 418
           M ++A+  EE+R   LK+ A+D+  E +     S         K     E   +S++E+ 
Sbjct: 369 MMENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMK----LESASTSSLEDR 425

BLAST of PI0010392 vs. ExPASy Swiss-Prot
Match: Q4X1D7 (Pre-mRNA-splicing factor cwc25 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-12
Identity = 128/417 (30.70%), Postives = 194/417 (46.52%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVSKQER 67
           KK WH   LRN E VW  E++   E+K++++LR++ +EE++ QE + LQE +G   +  R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIEQLRREREEERQIQELQRLQEASGKGRQLNR 67

Query: 68  LDFLYES-GLAVGK-ASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFEDKP-HS 127
           +D++Y++   A G  A   +G+   +                 K T V GA     P ++
Sbjct: 68  VDWMYQAPSSATGHYAEEMEGYLLGKRRIDGILLKNDESKKLEKGTDVVGANAAPPPVNN 127

Query: 128 ANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKH-KDKNPDDKRERKK 187
             DT  K+ +DPLL I++REQ A            M++++V   +H +DK+ D  RER++
Sbjct: 128 PRDTMAKVMADPLLEIKKREQAAYEN---------MVKESVRRSQHIRDKDRDRDRERRR 187

Query: 188 ----------------------------HRHSKSKRHKDSSP-------ERDCDPEDASP 247
                                       HRH +S RH+  SP        +  D  D   
Sbjct: 188 DRGDRDRSRDRDRSHRRSRHEDEDAHRSHRH-RSHRHRSRSPLSPDRSDRKRGDDRDYRD 247

Query: 248 ERQRRKHDHDKSSRHDGHSHSEDRRSKADSKNDR-DRDR-GSKYAARTS-YDQSDRKTSK 307
           ER RR    D+   H GH   +DRRS+ D + DR D DR  S+Y  R   YD S R+ S 
Sbjct: 248 ERDRR---DDRRDEHRGHDRRDDRRSERDRRADRGDEDRHSSRYKDRDDRYDHSTRRRSY 307

Query: 308 S--NPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEPRYYESN---ASETPHESRHRHRR 367
           +  +P     + ++DR +R  D      +DSR    P +Y  N    ++ P +S  R R 
Sbjct: 308 ADRSPSPRRGNHYYDR-RRTEDRSDGYHRDSRNHDRPEFYRGNDRKVNKEPRDSNLRERS 367

Query: 368 PTT---------KLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDALEAKQNAA 369
             T         K+ EEER  +L EM  +A   +E   KRL++ AE  A+E K+  A
Sbjct: 368 SNTRDEVAGNKEKVLEEERKKKLAEMMSNA---DELEQKRLQRIAEVTAMEEKEREA 407

BLAST of PI0010392 vs. ExPASy Swiss-Prot
Match: Q5B0I1 (Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.1e-12
Identity = 117/394 (29.70%), Postives = 185/394 (46.95%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVSKQER 67
           KK WH   LRN E VW  E++   E+K++D+L+++  EE++ QE + LQE AG   + +R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIDQLKRERDEERQIQELQRLQESAGGTRQLQR 67

Query: 68  LDFLYE-------------SGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVP 127
           +D++Y+              G  +GK    DG              AAT P ++    +P
Sbjct: 68  VDWMYQEPSSASGHYAEEMEGYLLGK-RRIDGILLKNDESKKLEKGAATGPGAA--AGLP 127

Query: 128 GALFEDKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV---EVEKHK 187
             +     H+  DT  K+ +DPLL IR+REQ A           AM++++V   E E+ +
Sbjct: 128 PVV-----HNTRDTMAKVMADPLLEIRKREQAAYE---------AMVKESVRRREREQGR 187

Query: 188 DKNPDDKRERKKHRH--------SKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRH 247
           +++ + +RER  HRH         +S RH+  S  R   P     +R+ R  +HD+ S  
Sbjct: 188 ERHQERERER-GHRHRRDSGNDSHRSHRHRRRSRSRSRSPRVEKDDRRHRDREHDRRSHR 247

Query: 248 DGHSHSEDRRSKADSKNDRDRDRGSKY---AARTSYDQSDRKTSKSNPHDSAADRHHDRS 307
           D     E+R  +    + RD DR  +Y   A R++  + DR+       D   D   DR+
Sbjct: 248 DRDGERENRVERVYHSSRRDHDRDERYDRAARRSASPRPDRRRDDRRDRDQDQDYRRDRN 307

Query: 308 KRD--RDSYANNDKDSRRVGEPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQ 367
            R   RD+ ++ D+D+   G  R      S + + +   +     +  EE+R  RL EMQ
Sbjct: 308 SRSSRRDARSSRDRDTDAFGRDR-NRDRGSHSRNGNADANADQHAQELEEQRKRRLAEMQ 367

Query: 368 QDAELHEEQRWKRLKKAAEDDALEAKQNAAPSSR 373
            +A+  E  R +R+   AE  ALE KQ      R
Sbjct: 368 ANAQDMEATRRQRV---AEVTALEEKQREEDDKR 379

BLAST of PI0010392 vs. ExPASy Swiss-Prot
Match: Q4P6I3 (Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CWC25 PE=3 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-09
Identity = 106/394 (26.90%), Postives = 182/394 (46.19%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVSKQER 67
           KK WH     N E VWK E++   E+KKL+ELR++ ++E+E Q+ + LQE+AG   + +R
Sbjct: 9   KKSWHPLLQVNQERVWKREKEALEERKKLEELRREREQEREMQQLQRLQEEAGGKKRIDR 68

Query: 68  LDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFEDKPHSAND 127
           +D++Y +    G  S+++    L             E     +T     +     +SA D
Sbjct: 69  VDWMYATPATTGSGSAAEMEDYLLGKKRVDKLLQGEENKEMSKTTQKSLVSLQNANSARD 128

Query: 128 TWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR--ERKKHR 187
              K+  DP+L I+Q+EQ A   +  +P ++  ++    ++       + +R  E K+ R
Sbjct: 129 LAAKIREDPMLAIKQQEQAAYEALLRDPTRLRQLKMQAGIDTDTQSKDERRRSKEEKRRR 188

Query: 188 HSKSK------RHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSK 247
           H + +      RH +S  E          +R+   ++   S RHDG    E+RRS    +
Sbjct: 189 HDRDEHRRRGSRHGESRSESRSHSRRDDRDRKSSWNEGSSSRRHDG---DEERRSLKRHR 248

Query: 248 NDRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRV-- 307
           +D+DRDR  +Y  R       R  S+SN      D   + S R   SY+ +   S     
Sbjct: 249 DDQDRDR-HRYERRLHDADDSRPASRSNRDRRDKDAVDESSSRQSTSYSRSRSRSPPARD 308

Query: 308 --GEPRYYESNASETP-HESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKK 367
              + R   S+A   P +    R+R    +  ++ R  +L+EM+Q+A   E+QR   + K
Sbjct: 309 YRNDKRLSSSSAFTRPSNGGSARNREEDEERKQQARQLKLQEMEQNARSMEQQRSSYVSK 368

Query: 368 AAEDDALEAKQNAAPSSRNFLDMAQKRMYGAEKG 389
              ++A + ++ A    R  L  A+ + +G  KG
Sbjct: 369 INAEEAEQERREA--ELRQKLIDARSKGHGDGKG 396

BLAST of PI0010392 vs. ExPASy TrEMBL
Match: A0A5A7TSZ0 (Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00360 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.5e-204
Identity = 402/419 (95.94%), Postives = 407/419 (97.14%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE A PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSSPERD D EDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSK--RDRDSYANNDKDSRRVG 300
           +RDRDRGSKYAARTSYDQSDRKTSKSNP DSAADRHHDRSK  RDRDS+ANNDKDSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360
           EPRYYES+ASETP+ESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           DALEAKQNA PSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of PI0010392 vs. ExPASy TrEMBL
Match: A0A1S3BY15 (pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.5e-204
Identity = 402/419 (95.94%), Postives = 407/419 (97.14%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE A PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSSPERD D EDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSK--RDRDSYANNDKDSRRVG 300
           +RDRDRGSKYAARTSYDQSDRKTSKSNP DSAADRHHDRSK  RDRDS+ANNDKDSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360
           EPRYYES+ASETP+ESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           DALEAKQNA PSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of PI0010392 vs. ExPASy TrEMBL
Match: A0A0A0KU62 (Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.6e-202
Identity = 396/417 (94.96%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE AVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSSPERD D ED SPERQRRKHDHDKSSRHDGHSHSEDRRSKA++KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEP 300
           +RDRDRGSKYAARTSYDQSDRKT KSNPHDSAADR+HDRSKRDRDSYA ND+DSRRVGE 
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDA 360
           RYYESNASETP+ESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQR+KRLKKA EDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           LEAKQNA PSSRNFLDMAQKRMY AEKGGSSTIEESIRRRTYYSQGKS+IE+NAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of PI0010392 vs. ExPASy TrEMBL
Match: A0A6J1CHZ1 (pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC111011671 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.2e-173
Identity = 356/417 (85.37%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKE A PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKH HSKSKRHKD S ERD D E+ASPERQRR HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEP 300
           +R  DRGSKYAA  S DQSDRKTSK++ H S A+RHHDRSKR+RDSYA+++K+SRRVGE 
Sbjct: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300

Query: 301 RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDA 360
           R YESN+SETPHESRH+HRR   KLSEEERAARLREMQQDAELH+EQRW+RLKKAAEDDA
Sbjct: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           LEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of PI0010392 vs. ExPASy TrEMBL
Match: A0A6J1E963 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430542 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 4.8e-169
Identity = 354/422 (83.89%), Postives = 369/422 (87.44%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLP-SSSTAAAATEPSSSKETAVPGALFE 120
           LV KQERLDFLYESGLAVGK S+SDGFKSLETLP ++STAAAA EPSSSKE A PGALFE
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR 180
           DKPHSAND WRKLHSDPLLIIRQREQ ALARVKNNPIQMAMIRKTVEVEKHK KNPDD+ 
Sbjct: 121 DKPHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEVEKHKAKNPDDEG 180

Query: 181 ERKKHRHSKSKRHKDSSPERDCDPEDASPERQRRK---HDHDKSSRHDGHSHSEDRRSKA 240
           ERKKH HSKSKR KDS P R+ D E ASPERQRRK   HD DKSS+ D  S+SEDRRSK 
Sbjct: 181 ERKKHCHSKSKRRKDSPPGREYDSESASPERQRRKRRNHDRDKSSKRDVRSYSEDRRSKT 240

Query: 241 DSKNDRDRDRGSKYAARTSYDQSDRKTSKSNPHD-SAADRHHDRSKRDRDSYANNDKDSR 300
           D  N+RDRD GSK      YDQSDRKT KSNPHD  A +RHHD SKR RDSY +N+KDSR
Sbjct: 241 DYMNERDRDSGSK------YDQSDRKTPKSNPHDYYAEERHHDSSKRYRDSYTHNEKDSR 300

Query: 301 RVGEPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKA 360
           RVGE R YE NAS TP E RH+HRRP TKLSEEERAARLREMQQDAE HEEQRW+RLKKA
Sbjct: 301 RVGESRTYEPNASNTPFEPRHKHRRPATKLSEEERAARLREMQQDAEFHEEQRWRRLKKA 360

Query: 361 AEDDALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAF 418
           AEDDALEA+QNA PSSRNFLD AQKRMY AEKGGSSTIEESIRRRTYYSQGKSEIE+NAF
Sbjct: 361 AEDDALEAEQNAVPSSRNFLDTAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSEIEANAF 416

BLAST of PI0010392 vs. NCBI nr
Match: XP_008453851.1 (PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa] >TYK16902.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 720.7 bits (1859), Expect = 7.3e-204
Identity = 402/419 (95.94%), Postives = 407/419 (97.14%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE A PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSSPERD D EDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSK--RDRDSYANNDKDSRRVG 300
           +RDRDRGSKYAARTSYDQSDRKTSKSNP DSAADRHHDRSK  RDRDS+ANNDKDSRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 EPRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360
           EPRYYES+ASETP+ESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           DALEAKQNA PSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of PI0010392 vs. NCBI nr
Match: XP_004146897.1 (pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetical protein Csa_014942 [Cucumis sativus])

HSP 1 Score: 712.6 bits (1838), Expect = 2.0e-201
Identity = 396/417 (94.96%), Postives = 405/417 (97.12%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE AVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSSPERD D ED SPERQRRKHDHDKSSRHDGHSHSEDRRSKA++KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEP 300
           +RDRDRGSKYAARTSYDQSDRKT KSNPHDSAADR+HDRSKRDRDSYA ND+DSRRVGE 
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDA 360
           RYYESNASETP+ESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQR+KRLKKA EDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           LEAKQNA PSSRNFLDMAQKRMY AEKGGSSTIEESIRRRTYYSQGKS+IE+NAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of PI0010392 vs. NCBI nr
Match: XP_038896474.1 (pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida])

HSP 1 Score: 695.3 bits (1793), Expect = 3.3e-196
Identity = 387/417 (92.81%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKE AVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHK+KNPD KRE
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKEKNPDGKRE 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKHRHSKSKRHKDSS ERD D EDASPERQRRKHDH+ SS+HD HSH EDRRSKADSKN
Sbjct: 181 RKKHRHSKSKRHKDSSSERDYDSEDASPERQRRKHDHENSSKHDAHSHLEDRRSKADSKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEP 300
           +RDRDRGSKYAART+YDQSDR+TSKSNPHDSAA+RHHDRS+RDRDSYANN+K+SRRVGEP
Sbjct: 241 ERDRDRGSKYAARTTYDQSDRRTSKSNPHDSAAERHHDRSRRDRDSYANNEKNSRRVGEP 300

Query: 301 RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDA 360
           R Y SNASET HESRH+HRRP TKLSEEER ARL EMQQDAELHEEQRW+RLKKAAEDDA
Sbjct: 301 RSYGSNASET-HESRHKHRRPATKLSEEERVARLHEMQQDAELHEEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           LEAKQNAAPSSRNFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 LEAKQNAAPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 416

BLAST of PI0010392 vs. NCBI nr
Match: XP_022141219.1 (pre-mRNA-splicing factor CWC25 homolog [Momordica charantia])

HSP 1 Score: 617.8 bits (1592), Expect = 6.6e-173
Identity = 356/417 (85.37%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALFED 120
           LV KQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKE A PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240
           RKKH HSKSKRHKD S ERD D E+ASPERQRR HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240

Query: 241 DRDRDRGSKYAARTSYDQSDRKTSKSNPHDSAADRHHDRSKRDRDSYANNDKDSRRVGEP 300
           +R  DRGSKYAA  S DQSDRKTSK++ H S A+RHHDRSKR+RDSYA+++K+SRRVGE 
Sbjct: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300

Query: 301 RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDDA 360
           R YESN+SETPHESRH+HRR   KLSEEERAARLREMQQDAELH+EQRW+RLKKAAEDDA
Sbjct: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360

Query: 361 LEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           LEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIE+NAFRR
Sbjct: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of PI0010392 vs. NCBI nr
Match: XP_023551879.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.9e-172
Identity = 355/418 (84.93%), Postives = 373/418 (89.23%), Query Frame = 0

Query: 2   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGL 61
           ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAGL
Sbjct: 7   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGL 66

Query: 62  VSKQERLDFLYESGLAVGKASSSDGFKSLETLP-SSSTAAAATEPSSSKETAVPGALFED 121
           V KQERLDFLYESGLAVGK S+SDGFKSLETLP ++STAAAATEPSSSKE A PGALFED
Sbjct: 67  VPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFED 126

Query: 122 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 181
           KPHSAND+WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE
Sbjct: 127 KPHSANDSWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 186

Query: 182 RKKHRHSKSKRHKDSSPERDCDPEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 241
           RKKHRHSKSKR K SSPERD D E  SPE QRR HD DKSS+HD HS+SEDRRSK D  N
Sbjct: 187 RKKHRHSKSKRRKASSPERDYDSESVSPEMQRRNHDRDKSSKHDVHSYSEDRRSKTDYVN 246

Query: 242 DRDRDRGSKYAARTSYDQSDRKTSKSNPHD-SAADRHHDRSKRDRDSYANNDKDSRRVGE 301
           +RDRDRGSK      YDQSDRKT KSNPHD  A +RHHD  KRDRDSY +N+KDS +VGE
Sbjct: 247 ERDRDRGSK------YDQSDRKTPKSNPHDYYAEERHHDSXKRDRDSYTHNEKDSTKVGE 306

Query: 302 PRYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAEDD 361
            R  E NAS++P+E RH+HRRP TKLSEE RAARLREMQQDAE HEEQRW+RLKKAAEDD
Sbjct: 307 SRTREPNASKSPYEPRHKHRRPATKLSEEGRAARLREMQQDAEFHEEQRWRRLKKAAEDD 366

Query: 362 ALEAKQNAAPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIESNAFRR 418
           ALEA+QNA PSSRNFLD AQKRMYGAEKGGSSTIEESI  RTYYSQGKSEIE+NAFRR
Sbjct: 367 ALEAEQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESICWRTYYSQGKSEIEANAFRR 418

BLAST of PI0010392 vs. TAIR 10
Match: AT2G44200.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 310.1 bits (793), Expect = 2.8e-84
Identity = 231/494 (46.76%), Postives = 292/494 (59.11%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           M +KFLNKKGWHTGSLRNIE VWKAEQK EAEQKKL+ELR QI +E+ER EFR LQEQAG
Sbjct: 1   MGMKFLNKKGWHTGSLRNIETVWKAEQKQEAEQKKLEELRLQILQERERSEFRALQEQAG 60

Query: 61  LVSKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSK-ETAVPGALFE 120
           L+ +QERL+FLY+SGLAVGK S+S    S +        + A   +S K + + PGALFE
Sbjct: 61  LIPRQERLEFLYDSGLAVGKGSASGSGVSFQKEEQPLAKSEAGSSASEKPDQSAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKR 180
           +K  SAND+WRKLHSDPLL+IRQREQ+ALA++KNNP++MA+IRK+VE EK K K+ D K 
Sbjct: 121 EKAQSANDSWRKLHSDPLLLIRQREQEALAKIKNNPVKMALIRKSVE-EKGKGKDGDTKE 180

Query: 181 ERKKHRHSKSKRHKDSSP-ERDCDPEDASPERQRRKHDHDKSS-RHDGH----------- 240
            +KKH+    K  K SS  +R    ED+  E   RK  H K+S  HD H           
Sbjct: 181 HKKKHKRKSGKHQKQSSSRQRSDSEEDSGEENNGRKSHHQKTSGTHDRHYERPRSDLEDE 240

Query: 241 --------SHSEDRRSKADSKNDRDRDRGSKYAARTSY--DQSDRKTSKSNPHD------ 300
                    H E RRS+ D  + R     + Y  R S   D+S R+ S+ N ++      
Sbjct: 241 SKGRESRDRHYEKRRSELDDGHKRRERHDTHYERRRSEMDDESKRRESRDNHYERRRSDL 300

Query: 301 -------SAADRHHDRSKRDRDSYAN-------------NDKDSRRVGEP---------- 360
                   + D+H +R + D D                 +D+  RR   P          
Sbjct: 301 DDESKRRESHDKHFERQRSDLDDEYKRRESQDKRRRSDIDDEPKRRDARPNEKYRNRSPK 360

Query: 361 -----------------RYYESNASETPHESRHRHRRPTTKLSEEERAARLREMQQDAEL 418
                            R  E   S  P+E ++R R+  +KLSEEERAARL++MQ DAE+
Sbjct: 361 GGVERENLKSYGQEDKKRKAEDLDSGKPNEYQNRRRKGGSKLSEEERAARLKQMQMDAEV 420

BLAST of PI0010392 vs. TAIR 10
Match: AT2G44195.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-32
Identity = 81/149 (54.36%), Postives = 99/149 (66.44%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGS+RN+E VWKAEQKHEAEQ+K++          ER EFR +QEQAG
Sbjct: 1   MALKFLNKKGWHTGSIRNVEKVWKAEQKHEAEQEKIE----------ERSEFRAIQEQAG 60

Query: 61  LVSKQ-ERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKETAVPGALF- 120
           LV ++ ERL+FLY+  LAV K          + + SS                +PGALF 
Sbjct: 61  LVPRRPERLEFLYDPELAVAK----------QNVSSSRHGVLFQNQDQRARATIPGALFD 120

Query: 121 -EDKPHSANDTWRKLHSDPLLIIRQREQQ 147
            +DK HSAND+WRK HSDPLL+IRQ+EQ+
Sbjct: 121 DDDKRHSANDSWRKFHSDPLLLIRQKEQE 129

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9DBF72.8e-1729.75Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV... [more]
Q9NXE86.3e-1729.86Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=... [more]
Q4X1D72.1e-1230.70Pre-mRNA-splicing factor cwc25 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A... [more]
Q5B0I16.1e-1229.70Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 381... [more]
Q4P6I32.8e-0926.90Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23... [more]
Match NameE-valueIdentityDescription
A0A5A7TSZ03.5e-20495.94Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BY153.5e-20495.94pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 P... [more]
A0A0A0KU629.6e-20294.96Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 ... [more]
A0A6J1CHZ13.2e-17385.37pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1E9634.8e-16983.89LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
XP_008453851.17.3e-20495.94PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 p... [more]
XP_004146897.12.0e-20194.96pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetica... [more]
XP_038896474.13.3e-19692.81pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida][more]
XP_022141219.16.6e-17385.37pre-mRNA-splicing factor CWC25 homolog [Momordica charantia][more]
XP_023551879.11.9e-17284.93LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT2G44200.12.8e-8446.76CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
AT2G44195.11.4e-3254.36CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..49
NoneNo IPR availableCOILSCoilCoilcoord: 346..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 193..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 384..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..329
NoneNo IPR availablePANTHERPTHR16196CELL CYCLE CONTROL PROTEIN CWF25coord: 1..417
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainSMARTSM01083Cir_N_3coord: 10..46
e-value: 3.9E-13
score: 59.6
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainPFAMPF10197Cir_Ncoord: 11..46
e-value: 8.0E-15
score: 54.8
IPR022209Pre-mRNA splicing factorPFAMPF12542CWC25coord: 64..155
e-value: 5.4E-12
score: 46.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010392.1PI0010392.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005684 U2-type spliceosomal complex