PI0010333 (gene) Melon (PI 482460) v1

Overview


Name	PI0010333
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	E3 ubiquitin ligase BIG BROTHER-like
Location	chr05: 22116858 .. 22121753 (-)
RNA-Seq Expression	PI0010333
Synteny	PI0010333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTTATTGTGGTAATGTGGATGGTGATGTAGTTGATCCAAAAAGTACCAAACTCCAACGTTACCTTTTGAGCCACGTCATTGCACATGCTTTGGTTAAAGGTTTCAATGACAGACAGCTTGTAAAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAACAACGTTTTCAGTTATAACTAATAAGCATCCAAACTTCTTCCTAATCGGTTTCCATTTTCACTCGTTACAGAGAATTCCAAAGCACAAAGAGGAAAGTAAAACTGCTCAACCTCTTCGCTCAACCAGCCATTGTCAGTTTCTTCACTAATTAATCTTTTCACGCTTTCGATGCATTCATTCTATATCTCTATTCCTTAATCTCTTTTCTTAATTGTGTTTTTTGATCAGTGATCTGCTCACTGTAAATTTGGCTTCGAGATCGCCGAAACTCTGTAATCTCCGCCTTGCCCGTGAGAAACTAGGTACTACAATGGAATAGTTCTTTTTTGTGCTGTTTATTGCTCGAGCTTAAATTTATGGATGGAATGTGGAGTATAGGAACGAATTGTTTTGTTTTGTGTGAAATAGTCGGTTGGAGTACGAATGTCTAGGCTCTATTTGTTGAATCTGATGCCGATTTATCTACATTCATGTTTTCTGTGGATTTCTACTTGGAGGCGATGAGTTGTAGTGTAGCAGATTTGATTTTCTCTTTCTCCTCGAGCTTTCTGATAGCTAAATGAAATAATGATATTTATGAAATGTCCACATTCGATGACAATATGATCCTGTAGAATATGGCTCTTTTTGTTTAATAGATTGGTGCTGCGGGAATTAAATTACAATTGTATCTCCTCCTGCTTGTTTCGTATCCACTTGTTTCTATGGAATATGATATAACATATAATCCTCCGTCAATCTCAAGATGAGATAGTCCTGTGATTTAATTACCATTTTAATTGCTTTATTTTTCTTGTTCATGTTTGCAGGAGTGGCTCAAGGACGTTCAGCTACTTTATTGAAGATCATTAGGAATAAATTCACTGCTTGAAGTTATGTCAAGAATAGGTTTGGAGAACTTTCAGGACGTTTAGGTTTGTTACTACTAGTGTATTTCCCCTTTGGGCTCTGATTCTTATGTTTGCGTATTACTTGTGCCTGAAGCAAATACTTATTGAAACAGAATTATCTAAAATGAGCTGGAACTCAAACATCGAGGTTCATTGTATGAATAACGGTTATCCTTACAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGTACTAAAAATCTTTTTTATTTTAGATGCTATCCATGAATGTCCTGTCTGGAAACTAGACTTATAGAACTCGTGGCATTTAAATGCTGCTTTATCTGTACCTTATAGACTTGCTTCTCTCTTCTGTAGATTTGTTGACAATTGTCTCAAGTAAAAAAAAGGAAAGCATGTGACATTTCATCTTCTAACTACTTTTGGCTTCTGAACTATGGCCGTTTCATTTTGCTCTAACTTCCTGATGCATGCTTGATTGGACATTTATTCTCAAGATCAAATGGTCTTTATATCTGATTTTCATGATTCCAAATTAATAAACTTATTTTCCCAACATGGAAGTATAATATTTTAATTTCATTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACTACAAGTTTGGGCATTCTGATTCTTGGAGCACATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCGATATTGATGAACATAGGAGGCCACTCTCGACAGTCCAGAATGAACAGAGTTTAGGAAATAGCGTGTGGGAAGAAGAAAATGTCAATCCTAATATGTCTGGTAACAGCATGGAATGTAAGGGTTAACATCTGTTTTGTTTCAAGCTTTTGAATGAACATTATAGTATGTTTGTTTGTTAAACAGAAGTTTCCAAGTTAAGGTAATGGAACAAACATTCAGATATTGAATGTTACGGTTCTATAAAATCACATGCTGGAAAGAAGATATTTAATGACTCTTTATTTAGATTGTTTTTCTCAGTTCATGAATTGAGGCATTTGTAAGAAAAAGCAGTTGATTTCATCCGTTTCTGGTTAATCGACAAACTTCATCTTTAGGCCATCTATTATTTTCCTCAGTTCATAAATGGTAATGTAATATGGTTCTAAGGGTTTTCTTATGCTACATGTAGAAAAATTGGCTGTAGGCATATTAGTCATGTTGTAAGATGGGTAACTTATTCAATGCAACAAATCAATAGGGTGACTTAACGAGTATCTTGTAGATTAGATACAATCTTTCTCAGTTCATAAACAATAATGTAATATATGTTCATTGGTTTTCTTATGCTACACGTAGAAAATGGCTTTAGACATAGTAGTCATGCTGTAAGATGGGCAACTTATTTGATGCAACAAATTGAATTTAGGGTGATTTAACGAGTATCCTGTAAATGAGCTACAATCACACCATCCATTAATACACATACCTTCTATCATATTTCAACGTTAACTTGTAATTACTTGGTGCATCTCATTTTACCAAGTACTATTATGTTGTAGTATTAACCATGCTACTAGGTATAGTATCAAAGATATGTACAAAATCTAACAGCAAAATCATAATACAGAAAAATCCAATTTTATATCATTTTATTATTCATGATTGTTTGCCCCTTTTCTTCTTTCTGATTCAATTAAAATGTTTATGGAAATTTAAATACCAGTTCCTGAACTCCGTATTTTTTTTTTTTTTTTACTTTTTCAGGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGGTCTGCAGCTTATCGATTCATAAACATGTTATGCCACTAAGTTTTCAGTTGTAGTGTTACTGCTAATTGTTAAGGTTGTTTTCGGTGGAGTACATCATTTTTGGTTTAGTGTCCCTGTATTTTGTGGTTCTTGCTGGCAATCAGTCACATGCTTGTGTTAATACATAAAATAAAATTATTCCTGTAAGATATAAAACTGGCTTATCCGAAATCACAGACTAAAGGAAAAGATATAGGATATTAGAATACAAATTCATTTCTAACCCAGTGTTGACAAACTTGGTGGCGGTTCAACTTCCTTAACATCTATTATTCCAGATTTTCTATTGAACCCCAAATAGACCTGGAATTTACTTAGTTCCAATACTTCAGAAGACATCTGTAAATGCGTGATTCCTAATCTGTTCCATTTTGAAATGTGCAGACTATTTGGCAAGATATTGTTGATCCTGATAACATGACTTATGAGGTTAGAGCTGCTGATTTTCTTTCCTATTTACTTTTGCATATTACTGAACAAAATGTTTTCTCCTGTAACTTTTATATGTTCTTATTGAAGCTATATTTGCTTCAATGGCTATTTATTATATGGATATTGCTAATTGAAATATTATATTACTCCCGGTCACTAAGAAGTCTTGCTGCTGTGTTGCAAAACCAGTAGCCATTACAGCTACTAGGCTGAAATTTGGCAGTAGGCTAGGTGTTGGGCACTTTCTTGGTAAGGTAGAAAGGAAAAAAATGATGGCACAAGTTAACCTAATTGTTTATTCTGCGTTCTGGTTGCTGGAACACTTCGACCTAATTTTCTATCCTTGAACTTAGCTTGCTTCCCTGGAGGGCCTTACATATATACCAAAGAAAGCTTGCTTCTTTGGAGCGCTCTGCCTGTTGACGCTGCTTTTTAAGAAGCTAAAATTGATTAGATAGGAGCATAGCTGCATAAGTAATCAGCTGTAAATCCACTCAATACATTCTGAAACCAAGTTCCATGTTTAATAAACGTAAAATTTGTCTGGCTAACTGCTATGTGTAAAGTCAACTATATGCCTCCTGAATTATGTCGATTACTCTCTTCAATAGAGTGACGGTTTAAAGAAACTTATTTCAGGAATTAGTAGATTTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTATAGGTGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTAACACTAGATTGATTGATTGTCTTATCTCGAAATATTTTAAACTTGTATTATTTTTCATTTGTTTTTTAGGATTGTTCCAGCTATCAAGATGTAGTAGTACTTACCAATGCCTTATACTTAGTAATGCCTATAACAAGTGGGGTATTGGGGGTTTGATAAGCAACGCATAATAATTATTGCCAAGCTATGTTTATTTTGACTTGTGTATATGACCTAATAGGCTAATAATGATGAACATAGTTCTTATATTTTTATTTTCATTATTATTGTTAAGAACAATATACAGTTACGACGAATGATCTTTCTCAATATTATCTCATTTACTCAAAATCATCTCAATAAAGAGAACAAGTGGTATGGACGACTAGCCTGCCTGCTATTTGCAACGGCACCCTTTATTCAACGAGGAAGAAGAAAATGATCAATAGATTCAAATTGGTGCATAAGCCTTCCTCAATAACATTTTTGAGTCATTACCGATTGACAATGATATATTTCTTGCATTAATATTTTTTAATTATATATATGAAGATACATCTTGATTTGTATACAGGTGTGTGATATGCCAAATGGAATACAAACGTGGAGATAAAAGAATTACTCTACCATGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGTGATTTTCTGATATCTTTTATCCCCCTCTTTTAGTAAAATATACAATCAGCAATTTCATTCAATATTGCATAATCTATCTGTTCCAGGCATGTCCCATTTGCTACACTGATGTTTTTGGTGACACTTCAAAACGATAA

mRNA sequence

ATGAGTGATCTGCTCACTGTAAATTTGGCTTCGAGATCGCCGAAACTCTGTAATCTCCGCCTTGCCCGTGAGAAACTAGGAGTGGCTCAAGGACGACGTTTAGAATTATCTAAAATGAGCTGGAACTCAAACATCGAGGTTCATTGTATGAATAACGGTTATCCTTACAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACTACAAGTTTGGGCATTCTGATTCTTGGAGCACATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCGATATTGATGAACATAGGAGGCCACTCTCGACAGTCCAGAATGAACAGAGTTTAGGAAATAGCGTGTGGGAAGAAGAAAATGTCAATCCTAATATGTCTGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGACTATTTGGCAAGATATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTATAGGTGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGAGATAAAAGAATTACTCTACCATGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGCATGTCCCATTTGCTACACTGATGTTTTTGGTGACACTTCAAAACGATAA

Coding sequence (CDS)

ATGAGTGATCTGCTCACTGTAAATTTGGCTTCGAGATCGCCGAAACTCTGTAATCTCCGCCTTGCCCGTGAGAAACTAGGAGTGGCTCAAGGACGACGTTTAGAATTATCTAAAATGAGCTGGAACTCAAACATCGAGGTTCATTGTATGAATAACGGTTATCCTTACAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCCGGTGCCTCACATTCTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACTACAAGTTTGGGCATTCTGATTCTTGGAGCACATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCGATATTGATGAACATAGGAGGCCACTCTCGACAGTCCAGAATGAACAGAGTTTAGGAAATAGCGTGTGGGAAGAAGAAAATGTCAATCCTAATATGTCTGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGACTATTTGGCAAGATATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTATAGGTGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGAGATAAAAGAATTACTCTACCATGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGCATGTCCCATTTGCTACACTGATGTTTTTGGTGACACTTCAAAACGATAA

Protein sequence

MSDLLTVNLASRSPKLCNLRLAREKLGVAQGRRLELSKMSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKFGHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDTSKR

Homology

[Close]

BLAST of PI0010333 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-44
Identity = 95/241 (39.42%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 46  EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKFGHSDSW 105
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N++ YKFG S S 
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 106 STSYFDAQSFEVQDHESDIDEHR-----RPLSTVQNEQSLGNSVWEEENV-NPNMSGNSM 165
           + S++   S+++ DH S +   R      P+  V ++    N+V     + + +    + 
Sbjct: 70  NASFYG--SYDMNDHLSRMSIGRTNWDYHPMVNVADDPE--NTVARSVQIGDTDEHSEAE 129

Query: 166 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 225
           EC     +    Q  WQD +DPD MTYEELV+LGE VGT+SRGL+QELI  LP  +Y++G
Sbjct: 130 ECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFG 189

Query: 226 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 280
               +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWLSINK CP+C ++VFG+ 
Sbjct: 190 SIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEP 246

[Close]

BLAST of PI0010333 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.2e-25
Identity = 54/112 (48.21%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 168 HEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRN 227
           H  Q  W D +DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   N
Sbjct: 229 HTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTN 288

Query: 228 ERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDTS 280
           E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  +V   TS
Sbjct: 289 ESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of PI0010333 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.0e-15
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 178 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 237
           +D DNM+YEEL+ LGE +G  S GL++E+I  +          +      E C +CQ EY
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 238 KRGDKRITLPCKHRYHTSCGTKWLSINKACPICYT 273
             GD   TL C H +HT+C  +WL +   CPIC T
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]

BLAST of PI0010333 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-13
Identity = 41/100 (41.00%), Postives = 56/100 (56.00%), Query Frame = 0


Query: 178 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKK----SRNERCVIC 237
           +D DNM+YEEL+ LGE +G  S GL++E+I L  + +++    S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 238 QMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTD 274
           Q EY  GD   TL C H +H  C  +W+ I   CPIC T+
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of PI0010333 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.9e-13
Identity = 38/93 (40.86%), Postives = 49/93 (52.69%), Query Frame = 0


Query: 178 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 237
           +D DNM+YEEL+ L E +G  S GL++E +  L   R    +        E C ICQ EY
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEY 627

Query: 238 KRGDKRITLPCKHRYHTSCGTKWLSINKACPIC 271
             GD   TL C H +H  C  +WL +   CPIC
Sbjct: 628 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of PI0010333 vs. ExPASy TrEMBL
Match: A0A0A0L1Z5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.4e-135
Identity = 236/249 (94.78%), Postives = 240/249 (96.39%), Query Frame = 0


Query: 33  RLELSKMSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN 92
           R+ELSKMSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN
Sbjct: 3   RIELSKMSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN 62

Query: 93  SSYYKFGHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPN 152
           SS YKFGHSD WS SYFDAQSFEVQDHES IDEHRRPLSTVQNEQS GNSVW EEN NPN
Sbjct: 63  SS-YKFGHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVW-EENANPN 122

Query: 153 MSGNSMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 212
           MSGNSMECPRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV
Sbjct: 123 MSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPV 182

Query: 213 SRYRWGFFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYT 272
           SRYRWGFFSRKKSRNERCVICQMEYKRGDK ITLPCKH+YHTSCGTKWLSI KACPICYT
Sbjct: 183 SRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 242

Query: 273 DVFGDTSKR 282
           +VFGDTSKR
Sbjct: 243 EVFGDTSKR 249

[Close]

BLAST of PI0010333 vs. ExPASy TrEMBL
Match: A0A1S3BNC2 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491520 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.9e-133
Identity = 231/243 (95.06%), Postives = 234/243 (96.30%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSSYYKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           G+SD WSTSYFDAQ FEVQDHES I EHRRPLSTVQNEQSLGNSVW EEN NPNMSGNSM
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVW-EENANPNMSGNSM 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
           ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVS YRWG
Sbjct: 121 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSSYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKHRYHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDT 240

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 242

[Close]

BLAST of PI0010333 vs. ExPASy TrEMBL
Match: A0A1S3BNM5 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491520 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.7e-128
Identity = 226/243 (93.00%), Postives = 229/243 (94.24%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSSYYKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           G+SD WSTSYFDAQ FEVQDHES I EHRRPLSTVQNEQSLGNSVW EEN NPNMSG   
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVW-EENANPNMSG--- 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
             PRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVS YRWG
Sbjct: 121 --PRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSSYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKHRYHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDT 237

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 237

[Close]

BLAST of PI0010333 vs. ExPASy TrEMBL
Match: A0A6J1K714 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 204/245 (83.27%), Postives = 222/245 (90.61%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFS  SHS+ETVCP+TNSSYYKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSAPSHSEETVCPATNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHR--RPLSTVQNEQSLGNSVWEEENVNPNMSGN 158
            H+DSWS S FD QSFEVQ HES IDEHR  +  STV +EQSLGN V  EENVNP+M+ N
Sbjct: 61  EHADSWSASCFDTQSFEVQGHESVIDEHRMQQEFSTVPDEQSLGNRV-REENVNPDMASN 120

Query: 159 SMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR 218
           SMECPRRHSNYHEYQTIWQDI+DPDNMTYEELVDLGETVG+QSRGL+QELIALLPVS+++
Sbjct: 121 SMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETVGSQSRGLSQELIALLPVSKFK 180

Query: 219 WGFFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFG 278
             FFSRKKSR ERCVICQM YK GD+RITLPCKH+YHTSCG+KWLSINKACPICYT+V+G
Sbjct: 181 CWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVYG 240

Query: 279 DTSKR 282
           D SKR
Sbjct: 241 DISKR 244

[Close]

BLAST of PI0010333 vs. ExPASy TrEMBL
Match: A0A6J1H1S6 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111459339 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.7e-115
Identity = 203/245 (82.86%), Postives = 219/245 (89.39%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCM NGYPYSTASFMEYFEGLTYDHVNFIFSG SHSQETVCP+TNSSYYKF
Sbjct: 1   MSWNSNIEVHCMTNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQETVCPATNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHR--RPLSTVQNEQSLGNSVWEEENVNPNMSGN 158
            H+DSWS S FD  SFEVQ HES IDEHR  +  S V +EQSLGN    EENVNP+M+ N
Sbjct: 61  EHADSWSASCFDTPSFEVQGHESAIDEHRMQQEFSPVPDEQSLGNRA-REENVNPDMASN 120

Query: 159 SMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYR 218
           SMECPRRHSNYHEYQTIWQDI+DPDNMTYEELVDLGETVG+QSRGL+QELIALLPVS+++
Sbjct: 121 SMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETVGSQSRGLSQELIALLPVSKFK 180

Query: 219 WGFFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFG 278
             FFSRKKSR ERCVICQM YK GD+RITLPCKH+YHTSCG+KWLSINKACPICYT+VFG
Sbjct: 181 CWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVFG 240

Query: 279 DTSKR 282
           D SKR
Sbjct: 241 DISKR 244

[Close]

BLAST of PI0010333 vs. NCBI nr
Match: XP_008449725.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 231/243 (95.06%), Postives = 234/243 (96.30%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSSYYKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           G+SD WSTSYFDAQ FEVQDHES I EHRRPLSTVQNEQSLGNSVW EEN NPNMSGNSM
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVW-EENANPNMSGNSM 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
           ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVS YRWG
Sbjct: 121 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSSYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKHRYHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDT 240

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 242

[Close]

BLAST of PI0010333 vs. NCBI nr
Match: XP_004142225.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_011653590.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739641.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739642.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >KAE8649510.1 hypothetical protein Csa_017925 [Cucumis sativus])

HSP 1 Score: 483.0 bits (1242), Expect = 1.7e-132
Identity = 231/243 (95.06%), Postives = 234/243 (96.30%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           GHSD WS SYFDAQSFEVQDHES IDEHRRPLSTVQNEQS GNSVW EEN NPNMSGNSM
Sbjct: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVW-EENANPNMSGNSM 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
           ECPRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG
Sbjct: 121 ECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKH+YHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDT 240

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 241

[Close]

BLAST of PI0010333 vs. NCBI nr
Match: XP_031739643.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X2 [Cucumis sativus])

HSP 1 Score: 476.1 bits (1224), Expect = 2.1e-130
Identity = 230/243 (94.65%), Postives = 233/243 (95.88%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ TVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ-TVCPSTNSS-YKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           GHSD WS SYFDAQSFEVQDHES IDEHRRPLSTVQNEQS GNSVW EEN NPNMSGNSM
Sbjct: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVW-EENANPNMSGNSM 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
           ECPRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG
Sbjct: 121 ECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKH+YHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDT 240

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 240

[Close]

BLAST of PI0010333 vs. NCBI nr
Match: XP_008449726.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo])

HSP 1 Score: 467.2 bits (1201), Expect = 9.8e-128
Identity = 226/243 (93.00%), Postives = 229/243 (94.24%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSSYYKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           G+SD WSTSYFDAQ FEVQDHES I EHRRPLSTVQNEQSLGNSVW EEN NPNMSG   
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVW-EENANPNMSG--- 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
             PRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVS YRWG
Sbjct: 121 --PRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSSYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKHRYHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHTSCGTKWLSIKKACPICYTEVFGDT 237

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 237

[Close]

BLAST of PI0010333 vs. NCBI nr
Match: XP_031739644.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X3 [Cucumis sativus])

HSP 1 Score: 465.7 bits (1197), Expect = 2.8e-127
Identity = 226/243 (93.00%), Postives = 229/243 (94.24%), Query Frame = 0


Query: 39  MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKF 98
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60

Query: 99  GHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLGNSVWEEENVNPNMSGNSM 158
           GHSD WS SYFDAQSFEVQDHES IDEHRRPLSTVQNEQS GNSVW EEN NPNMSG   
Sbjct: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVW-EENANPNMSG--- 120

Query: 159 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 218
             PRRHSNYHEYQTIWQ+IVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG
Sbjct: 121 --PRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 180

Query: 219 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 278
           FFSRKKSRNERCVICQMEYKRGDK ITLPCKH+YHTSCGTKWLSI KACPICYT+VFGDT
Sbjct: 181 FFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDT 236

Query: 279 SKR 282
           SKR
Sbjct: 241 SKR 236

[Close]

BLAST of PI0010333 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 95/241 (39.42%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 46  EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKFGHSDSW 105
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N++ YKFG S S 
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 106 STSYFDAQSFEVQDHESDIDEHR-----RPLSTVQNEQSLGNSVWEEENV-NPNMSGNSM 165
           + S++   S+++ DH S +   R      P+  V ++    N+V     + + +    + 
Sbjct: 70  NASFYG--SYDMNDHLSRMSIGRTNWDYHPMVNVADDPE--NTVARSVQIGDTDEHSEAE 129

Query: 166 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 225
           EC     +    Q  WQD +DPD MTYEELV+LGE VGT+SRGL+QELI  LP  +Y++G
Sbjct: 130 ECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFG 189

Query: 226 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 280
               +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWLSINK CP+C ++VFG+ 
Sbjct: 190 SIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEP 246

[Close]

BLAST of PI0010333 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 95/241 (39.42%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 46  EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYYKFGHSDSW 105
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N++ YKFG S S 
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 106 STSYFDAQSFEVQDHESDIDEHR-----RPLSTVQNEQSLGNSVWEEENV-NPNMSGNSM 165
           + S++   S+++ DH S +   R      P+  V ++    N+V     + + +    + 
Sbjct: 70  NASFYG--SYDMNDHLSRMSIGRTNWDYHPMVNVADDPE--NTVARSVQIGDTDEHSEAE 129

Query: 166 ECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWG 225
           EC     +    Q  WQD +DPD MTYEELV+LGE VGT+SRGL+QELI  LP  +Y++G
Sbjct: 130 ECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFG 189

Query: 226 FFSRKKSRNERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDT 280
               +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWLSINK CP+C ++VFG+ 
Sbjct: 190 SIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEP 246

[Close]

BLAST of PI0010333 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 2.3e-26
Identity = 54/112 (48.21%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 168 HEYQTIWQDIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRN 227
           H  Q  W D +DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   N
Sbjct: 229 HTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTN 288

Query: 228 ERCVICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVFGDTS 280
           E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  +V   TS
Sbjct: 289 ESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of PI0010333 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.7e-21
Identity = 49/105 (46.67%), Postives = 65/105 (61.90%), Query Frame = 0


Query: 179 DPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRY-----RWGFFSRKK---SRNERC 238
           DP+NMTYEEL +LG++VG   +GL+QE I+ L   +Y      W     KK   + + +C
Sbjct: 175 DPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQC 234

Query: 239 VICQMEYKRGDKRITLPCKHRYHTSCGTKWLSINKACPICYTDVF 276
            IC MEY +GDK  TLPCKH YH  C ++WL  NK C IC  +V+
Sbjct: 235 SICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of PI0010333 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 3.6e-19
Identity = 64/197 (32.49%), Postives = 103/197 (52.28%), Query Frame = 0


Query: 81  SHSQETVCPSTNSSYYKFGHSDSWSTSYFDAQSFEVQDHESDIDEHRRPLSTVQNEQSLG 140
           ++S+  +  S  +S++     DS  +     +S E  + E +I+E     +  +   S G
Sbjct: 24  ANSEVALAASQANSHFAHAMHDSSPSMISMIESDEESEDEEEINE-----NYYEYFDSNG 83

Query: 141 NSVWEEENVNPNMSGNSMECPRRHSNYHEYQTIW--QDIVDPDNMTYEELVDLGETVGTQ 200
             V +E+ +N       +E    +SN  E       +D +DPD ++YEEL+ LG+ +G +
Sbjct: 84  FGV-DEDEIN-----EFLEDQESNSNLEEEDDFLEEEDEIDPDQLSYEELIALGDFIGVE 143

Query: 201 SRGLTQ-ELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKRITL-PCKHRYHTSC 260
           +RGLT  E+   L  S Y    FS  K+  +RCV+CQME++  +  + L PC H YH+ C
Sbjct: 144 NRGLTPIEISTCLNASTY---VFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSEC 203

Query: 261 GTKWLSINKACPICYTD 274
            TKWL   K CPIC ++
Sbjct: 204 ITKWLETKKICPICCSE 206

The following BLAST results are available for this feature:

BLAST of PI0010333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	3.1e-44	39.42	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	3.2e-25	48.21	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	3.0e-15	41.05	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q9ZQF9	1.3e-13	41.00	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	2.9e-13	40.86	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of PI0010333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1Z5	1.4e-135	94.78	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 P...	[more]
A0A1S3BNC2	2.9e-133	95.06	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3BNM5	4.7e-128	93.00	E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1K714	2.4e-116	83.27	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765...	[more]
A0A6J1H1S6	2.7e-115	82.86	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114593...	[more]

BLAST of PI0010333 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008449725.1	5.9e-133	95.06	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo]	[more]
XP_004142225.1	1.7e-132	95.06	E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_0116535...	[more]
XP_031739643.1	2.1e-130	94.65	E3 ubiquitin-protein ligase BIG BROTHER isoform X2 [Cucumis sativus]	[more]
XP_008449726.1	9.8e-128	93.00	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo]	[more]
XP_031739644.1	2.8e-127	93.00	E3 ubiquitin-protein ligase BIG BROTHER isoform X3 [Cucumis sativus]	[more]

BLAST of PI0010333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	2.2e-45	39.42	RING/U-box superfamily protein	[more]
AT3G63530.2	2.2e-45	39.42	RING/U-box superfamily protein	[more]
AT3G19910.1	2.3e-26	48.21	RING/U-box superfamily protein	[more]
AT5G52140.1	1.7e-21	46.67	RING/U-box superfamily protein	[more]
AT3G47180.1	3.6e-19	32.49	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 230..270 e-value: 8.8E-6 score: 35.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 229..270 e-value: 8.4E-11 score: 42.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 230..270 score: 11.013978
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 192..275 e-value: 5.7E-17 score: 63.2
None	No IPR available	PANTHER	PTHR46400:SF5	RING/U-BOX SUPERFAMILY PROTEIN	coord: 39..280
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 229..275
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 39..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0010333.1	PI0010333.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity