PI0010128 (gene) Melon (PI 482460) v1

Overview
NamePI0010128
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr06: 1858584 .. 1861122 (-)
RNA-Seq ExpressionPI0010128
SyntenyPI0010128
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTTTCCCTTCACTACATTTCTTCTTCTTCTCTTCCTACCCCTTGTGGCTTCTTCTAATTTTTCATTGCCTGCGCTGGACCCGGTTCGGTTGGCGCAGACCGGGTTAGGGAAGCCATAACTGAAATTGGTAATTTAAAACGACGTCGGCTTGGAGATTCGAATCTGCAGGCCGCGCTTCGTGATTGCTCTACGCTCTATGAGGAGGCCGAGTGGCGGCTCGCAGGGATGTTGGTTGGAGAGAATTATAGGGCGGAAGATGGTCGGATGTGGGTCAGCGCCGCCGTGGCTAACCATCGGAGTTGTTTGGATGGATTGGAAGAGGTCGGTGGTGGGGCGGCGGTGGATGGGAATAACTTGACAGTGATGCTCACCGGAGCTCTGCATTTATACGATAAGATTGCTGCCGTGGAGAAGCGGAACGGTGAGCACTTGCTGCCCACGTTGCAATCACCATTCCATTTTCCAACTTATTTATTTTTGTTATATATTGGTTCAACTTATTTATATATATATATATATATATATATGTATGTATGTATGTATATAATGGTTAAAAAATCCTAAAATTTCATAGAAGCAAAAATTTAATCTATCAGTTTTGGTGGGTAACAATTGAGTTTTAAATTAAATTATATAAGGATATCTTTGAAAAAAATTATCAATTTTTAAATTTAAAAACAAGTTATTTGGAATAAAATTAGAGCAATCGTGGAAGATAATTTTTTAATAATATTTGAAATAGTTTTTATTAAAAGAGTACGGACAAAAATGAGTTTTTTTTTTTTTTTTAAATATCAAGTCAATTCAAACAAACCCATTAGTTTAAGAATTGTAGGTGGAAGCATTTTGTCATTTTTGTTTAAATTGGTGGAAAAAAGTTCTCGATAATTTTATAAAACACAAAATCTATTCACAGAATAATAAAAATTGACCTAAATATTGGTTTCAAACCGTTGAAAGAAATACAGAACCTAAAAATAGATGCAATAGACTTTTACCTATCGAATAATCTAAAACTAATTAACATAATGTTTATAAAATGATTATACCATATTTTATAACATTAATGTTGAATACTAATTTGCTATTACATTGCTATTTATACAATAATCTAACAGACAAAGATAGTATTTTAACATATTATTTAAAAAAAATTATTATTATTTTTTTCTCAAGTCCCTTTTTGCATTAATTTCTAATTCTGATTTGGTGAGTACGTATTATTCAAAATGGAAAAAAAAAATGATAATAATAAAAACTGATAGACCAAAATAGAAGAAATAAAATAAAAGACATAAAAGACTTTCATTTCACGTAAAGAAATGGAAATTTGAATTCAGGATGGAAAAGATTGGAGAAACGGAAATGGAGAGAAAACAGAGGAATCAACTTGGCAACGTGGAATCAGGCGACGTCGAAGGCGGACTATGTGGTGGCGAAGGATGGATCCGGCACCCACAGAACCATAAACGGGGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGGCGGGGAGGGAGAATTGTGATATACGTGAAAGCTGGAGTTTACCGGGAGAATGTGGAAATTGGAATACAGTCGAAGAACGTTATGCTTGTCGGCGACGGCACAGACAAAACCATTGTCACCGGTAGCCGCAATGTACCCGACGGCGCCACCACTTATAACTCAGCTACATTCGGTAAGCCATATCTTTAAAAAATACGAATGCGTAAATAACTATATGTATATATATATATATATAACATTTTCCTTTTTCTTTAATGCAGTAACGAAAATTTTGTAATATTCTTTTGGTTTTGAAATATAAAATGTTTGATAGATTTGCCGTATCTGAGCAGGCGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCCGGACCGGGAAAGCATCAAGCGGTGGCGCTACGGCTCAATTCCGACCTTGCCGTCGTATACCGGTGCGCAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTTCGCCAATTCTACCGCGACTGCCAAATCTACGGCACCATCGATTTCATCTTTGGCAATTCGGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGACAGCCGATTCGACACCAGGCCAACATGATCACCGCCCAAGGCCGGGACGATCCGGCAGAGGCCACCGGAATTTCGATCCTCAACTCCAGAGTCCAGCCGACGCCGGATTTTGCCCCCGTAAAGGGTCAGTACAGGACGTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTAATGGCGACGGATTTGGACGGGTTGATTGATCCGAAAGGGTGGGGAGAATGGACAGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAAGGGCGACGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCTGCACTACGGAGGAAGCGCGGCCTTTCACGGTGGCTGAGTTTCTTCACGGCGGCGATTGGATTCCGACCACCGGCGTACCCTTCGAGGCGGGAGTTTGA

mRNA sequence

ATGCCCTTTCCCTTCACTACATTTCTTCTTCTTCTCTTCCTACCCCTTGTGGCTTCTTCTAATTTTTCATTGCCTGCGCTGGACCCGGCCGCGCTTCGTGATTGCTCTACGCTCTATGAGGAGGCCGAGTGGCGGCTCGCAGGGATGTTGGTTGGAGAGAATTATAGGGCGGAAGATGGTCGGATGTGGGTCAGCGCCGCCGTGGCTAACCATCGGAGTTGTTTGGATGGATTGGAAGAGGTCGGTGGTGGGGCGGCGGTGGATGGGAATAACTTGACAGTGATGCTCACCGGAGCTCTGCATTTATACGATAAGATTGCTGCCGTGGAGAAGCGGAACGGATGGAAAAGATTGGAGAAACGGAAATGGAGAGAAAACAGAGGAATCAACTTGGCAACGTGGAATCAGGCGACGTCGAAGGCGGACTATGTGGTGGCGAAGGATGGATCCGGCACCCACAGAACCATAAACGGGGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGGCGGGGAGGGAGAATTGTGATATACGTGAAAGCTGGAGTTTACCGGGAGAATGTGGAAATTGGAATACAGTCGAAGAACGTTATGCTTGTCGGCGACGGCACAGACAAAACCATTGTCACCGGTAGCCGCAATGTACCCGACGGCGCCACCACTTATAACTCAGCTACATTCGGCGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCCGGACCGGGAAAGCATCAAGCGGTGGCGCTACGGCTCAATTCCGACCTTGCCGTCGTATACCGGTGCGCAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTTCGCCAATTCTACCGCGACTGCCAAATCTACGGCACCATCGATTTCATCTTTGGCAATTCGGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGACAGCCGATTCGACACCAGGCCAACATGATCACCGCCCAAGGCCGGGACGATCCGGCAGAGGCCACCGGAATTTCGATCCTCAACTCCAGAGTCCAGCCGACGCCGGATTTTGCCCCCGTAAAGGGTCAGTACAGGACGTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTAATGGCGACGGATTTGGACGGGTTGATTGATCCGAAAGGGTGGGGAGAATGGACAGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAAGGGCGACGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCTGCACTACGGAGGAAGCGCGGCCTTTCACGGTGGCTGAGTTTCTTCACGGCGGCGATTGGATTCCGACCACCGGCGTACCCTTCGAGGCGGGAGTTTGA

Coding sequence (CDS)

ATGCCCTTTCCCTTCACTACATTTCTTCTTCTTCTCTTCCTACCCCTTGTGGCTTCTTCTAATTTTTCATTGCCTGCGCTGGACCCGGCCGCGCTTCGTGATTGCTCTACGCTCTATGAGGAGGCCGAGTGGCGGCTCGCAGGGATGTTGGTTGGAGAGAATTATAGGGCGGAAGATGGTCGGATGTGGGTCAGCGCCGCCGTGGCTAACCATCGGAGTTGTTTGGATGGATTGGAAGAGGTCGGTGGTGGGGCGGCGGTGGATGGGAATAACTTGACAGTGATGCTCACCGGAGCTCTGCATTTATACGATAAGATTGCTGCCGTGGAGAAGCGGAACGGATGGAAAAGATTGGAGAAACGGAAATGGAGAGAAAACAGAGGAATCAACTTGGCAACGTGGAATCAGGCGACGTCGAAGGCGGACTATGTGGTGGCGAAGGATGGATCCGGCACCCACAGAACCATAAACGGGGCGGTGGCGGCTCTGGCGAGGACTGGACGGAGACGGCGGGGAGGGAGAATTGTGATATACGTGAAAGCTGGAGTTTACCGGGAGAATGTGGAAATTGGAATACAGTCGAAGAACGTTATGCTTGTCGGCGACGGCACAGACAAAACCATTGTCACCGGTAGCCGCAATGTACCCGACGGCGCCACCACTTATAACTCAGCTACATTCGGCGTTTCCGGCGACGGATTTTGGGCGAGGGACATAACATTCGAGAACACCGCCGGACCGGGAAAGCATCAAGCGGTGGCGCTACGGCTCAATTCCGACCTTGCCGTCGTATACCGGTGCGCAATAAAAGGCTATCAGGACACCCTCTTCCTCCACTCCCTTCGCCAATTCTACCGCGACTGCCAAATCTACGGCACCATCGATTTCATCTTTGGCAATTCGGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGACAGCCGATTCGACACCAGGCCAACATGATCACCGCCCAAGGCCGGGACGATCCGGCAGAGGCCACCGGAATTTCGATCCTCAACTCCAGAGTCCAGCCGACGCCGGATTTTGCCCCCGTAAAGGGTCAGTACAGGACGTACCTCGGCCGCCCCTGGAAGCGGTTTTCGAGGACGGTGGTAATGGCGACGGATTTGGACGGGTTGATTGATCCGAAAGGGTGGGGAGAATGGACAGGGGATTTTGCGATTTCGACTCTGTTTTATGCAGAGTTTAAGAACAAGGGCGACGGTTCTTCGACGAGATTTAGGGTTAATTGGCCGGGATTTCACGTCCTCTGCACTACGGAGGAAGCGCGGCCTTTCACGGTGGCTGAGTTTCTTCACGGCGGCGATTGGATTCCGACCACCGGCGTACCCTTCGAGGCGGGAGTTTGA

Protein sequence

MPFPFTTFLLLLFLPLVASSNFSLPALDPAALRDCSTLYEEAEWRLAGMLVGENYRAEDGRMWVSAAVANHRSCLDGLEEVGGGAAVDGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTGVPFEAGV
Homology
BLAST of PI0010128 vs. ExPASy Swiss-Prot
Match: Q84R10 (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3702 GN=PME36 PE=2 SV=2)

HSP 1 Score: 525.4 bits (1352), Expect = 6.4e-148
Identity = 261/452 (57.74%), Postives = 328/452 (72.57%), Query Frame = 0

Query: 32  LRDCSTLYEEAEWRLAGMLVG-ENYRAEDGRMWVSAAVANHRSCLDGL-EEVGGGAAVDG 91
           L +C  LY+E+E RL+ ++V  EN+  ED R W+S  +ANH +CLDGL ++  G   +  
Sbjct: 69  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 128

Query: 92  NNLTVMLTGALHLYDKIAAVEKR---------NGWKRLEKRKWRENRGIN---------- 151
           +N+T +L  AL  Y K     K+         +G  R + R  R N G            
Sbjct: 129 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 188

Query: 152 -----LATWNQATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYR 211
                L +WN  +S+AD+VVA+DGS THRTIN A+AA++R G+ R   R++IY+KAGVY 
Sbjct: 189 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRL-NRVIIYIKAGVYN 248

Query: 212 ENVEIGIQSKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTA 271
           E +EI    KN+MLVGDG D+TIVT +RNVPDG+TTY SATFGVSGDGFWARDITFENTA
Sbjct: 249 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTA 308

Query: 272 GPGKHQAVALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVL 331
           GP KHQAVALR++SDL++ YRC+ KGYQDTLF HSLRQFYRDC IYGTIDFIFG++AAV 
Sbjct: 309 GPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVF 368

Query: 332 QNCEIFVRQPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPW 391
           QNC+IFVR+P+ HQ NMITAQGRDDP   +GISI +SR++  P+F  VKG++++YLGRPW
Sbjct: 369 QNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPW 428

Query: 392 KRFSRTVVMATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVL 451
           K++SRTV + TD+D LIDP+GW EW+G +A+STL+Y EF N G G+ T  RVNWPGFHVL
Sbjct: 429 KKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVL 488

Query: 452 CTTEEARPFTVAEFLHGGDWIPTTGVPFEAGV 458
              EEA PFTV+ F+ G  WIP TGVPF AGV
Sbjct: 489 RGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

BLAST of PI0010128 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-109
Identity = 213/438 (48.63%), Postives = 277/438 (63.24%), Query Frame = 0

Query: 28  DPAALRDCSTLYEEAEWRL-AGMLVGENYRAEDGRMWVSAAVANHRSCLDGLEEVGGGAA 87
           + AA  DC  LYE    +L             D + W+S A+ N  +C   LE++G    
Sbjct: 91  EKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLGVPEY 150

Query: 88  V---DGNNLTVMLTGALHL----YDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATS 147
           V     NN+T +++  L L    Y++ +  +    W +   RK             Q T 
Sbjct: 151 VLPLLSNNVTKLISNTLSLNKVPYNEPSYKDGFPTWVKPGDRKLL-----------QTTP 210

Query: 148 KADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVML 207
           +A+ VVA+DGSG  +TI  AVAA +R G    G R VIY+KAG Y EN+E+ +  KN+M 
Sbjct: 211 RANIVVAQDGSGNVKTIQEAVAAASRAG----GSRYVIYIKAGTYNENIEVKL--KNIMF 270

Query: 208 VGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNS 267
           VGDG  KTI+TGS++V  GATT+ SAT  V GD F ARDIT  NTAGP  HQAVALR  S
Sbjct: 271 VGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGS 330

Query: 268 DLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQ 327
           DL+V YRC+ +GYQDTL++HS RQFYR+C IYGT+DFIFGN+A VLQNC IF R+P  ++
Sbjct: 331 DLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKP-PNR 390

Query: 328 ANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLD 387
            N +TAQGR DP ++TGI I N RV    D  PV+   +T+LGRPWK++SRTV + T LD
Sbjct: 391 TNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLD 450

Query: 388 GLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEF 447
            LI+P GW EW+GDFA++TL+YAE+ N G GSST  RV W G+HVL +  +   FTV  F
Sbjct: 451 SLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNF 510

Query: 448 LHGGDWIPTTGVPFEAGV 458
           + G  W+P T VPF +G+
Sbjct: 511 IAGNSWLPATNVPFTSGL 510

BLAST of PI0010128 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 388.7 bits (997), Expect = 9.4e-107
Identity = 219/441 (49.66%), Postives = 280/441 (63.49%), Query Frame = 0

Query: 28  DPAALRDCSTLYEEAEWRLAGMLVGENYRAE--DGRMWVSAAVANHRSCLDGLEEVGGGA 87
           + AA  DC  LY+    ++    +  N +    D + W+S A+ N  +C  G  E+G   
Sbjct: 93  EKAAWEDCIKLYDLTVSKI-NETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTD 152

Query: 88  AV---DGNNLTVMLTGALHLYDKIA---AVEKRNG---WKRLEKRKWRENRGINLATWNQ 147
            V     NN++ +L   L + +K+       +++G   W +   RK  ++          
Sbjct: 153 IVLPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPGDRKLLQS---------- 212

Query: 148 ATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKN 207
           +T K + VVAKDGSG  +TI  A+ A + +      GR VIYVK GVY EN+E  I+ KN
Sbjct: 213 STPKDNAVVAKDGSGNFKTIKEAIDAASGS------GRFVIYVKQGVYSENLE--IRKKN 272

Query: 208 VMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALR 267
           VML GDG  KTI+TGS++V  G TT+NSAT    GDGF AR ITF NTAG    QAVALR
Sbjct: 273 VMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALR 332

Query: 268 LNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPI 327
             SDL+V Y+C+ + YQDTL++HS RQFYRDC +YGT+DFIFGN+AAVLQNC IF R+P 
Sbjct: 333 SGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP- 392

Query: 328 RHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMAT 387
           R + N ITAQGR DP + TGI I NSRV    D  PV G  +TYLGRPW+++SRTV M T
Sbjct: 393 RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKT 452

Query: 388 DLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTV 447
            LD LIDP+GW EW G+FA+ TLFYAEF+N G G+ST  RV WPGF VL +  EA  FTV
Sbjct: 453 SLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTV 511

Query: 448 AEFLHGGDWIPTTGVPFEAGV 458
             FL GG WIP++ VPF +G+
Sbjct: 513 GTFLAGGSWIPSS-VPFTSGL 511

BLAST of PI0010128 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 9.4e-107
Identity = 211/446 (47.31%), Postives = 270/446 (60.54%), Query Frame = 0

Query: 30  AALRDCSTLYEEAEWRLAGMLVGENYRAE--------DGRMWVSAAVANHRSCLDGLEEV 89
           A L DC  LY +   +L   L G + +A         D + W+S A+ N  +C  G  ++
Sbjct: 99  AVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDL 158

Query: 90  GGGAAVDGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRG-INLATWNQATSK 149
                 D     V  T   HL     AV   NG       K           TW     K
Sbjct: 159 N---VTDFITPIVSNTKISHLISNCLAV---NGALLTAGNKGNTTANQKGFPTWLSRKDK 218

Query: 150 -------ADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQ 209
                  A+ VVAKDGSG   T+  A+    R  R+   GR VIYVK G+Y+EN+ + + 
Sbjct: 219 RLLRAVRANLVVAKDGSGHFNTVQAAIDVAGR--RKVTSGRFVIYVKRGIYQENINVRLN 278

Query: 210 SKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAV 269
           + ++MLVGDG   TI+TG R+V  G TTYNSAT G+ G  F A+ ITF NTAGP K QAV
Sbjct: 279 NDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAV 338

Query: 270 ALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVR 329
           ALR +SDL++ Y+C+I+GYQDTL +HS RQFYR+C IYGT+DFIFGN+AAV QNC I  R
Sbjct: 339 ALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPR 398

Query: 330 QPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVV 389
           +P++ QAN+ITAQGR DP + TGISI NSR+ P PD  PV G  +TY+GRPW +FSRTVV
Sbjct: 399 RPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVV 458

Query: 390 MATDLDGLIDPKGWGEWT--GDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEA 449
           + T LD ++ P GW  W     F + TLFYAE+KN G  SSTR+RV+W GFHVL    +A
Sbjct: 459 LQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDA 518

Query: 450 RPFTVAEFLHGGDWIPTTGVPFEAGV 458
             FTV +F+ G  W+P TG+PF +G+
Sbjct: 519 SAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of PI0010128 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.1e-104
Identity = 204/448 (45.54%), Postives = 278/448 (62.05%), Query Frame = 0

Query: 31  ALRDCSTLYEEAEWRLA---GMLVGENYRA---EDGRMWVSAAVANHRSCLDGLEEVGGG 90
           AL DC+ L + ++ R+     +L  +N      ED  +W+S  + NH +CLDGLEE G  
Sbjct: 109 ALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEE-GST 168

Query: 91  AAVDG------NNLTVMLTGALHLY-----DKIAAVEKRNG----WKRLEKRKWRENRGI 150
             +        N L +    +L ++      K   +E   G    W     R+  +  G 
Sbjct: 169 DYIKTLMESHLNELILRARTSLAIFVTLFPAKSNVIEPVTGNFPTWVTAGDRRLLQTLGK 228

Query: 151 NLATWNQATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVE 210
           ++        + D VVAKDGSG + T+N AVAA+    ++    R+++ V+ G+Y ENV+
Sbjct: 229 DI--------EPDIVVAKDGSGDYETLNEAVAAIPDNSKK----RVIVLVRTGIYEENVD 288

Query: 211 IGIQSKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGK 270
            G Q KNVMLVG+G D TI+TGSRNV DG+TT++SAT    GDGF A+DI F+NTAGP K
Sbjct: 289 FGYQKKNVMLVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEK 348

Query: 271 HQAVALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCE 330
           +QAVALR+ +D  V+ RC I  YQDTL+ H+ RQFYRD  I GT+DFIFGN+A V QNC 
Sbjct: 349 YQAVALRIGADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCN 408

Query: 331 IFVRQPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFS 390
           +  R+ ++ Q N ITAQGR DP + TG SI N  +  + D  PV+  +++YLGRPWK +S
Sbjct: 409 LIPRKQMKGQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYS 468

Query: 391 RTVVMATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTE 450
           RTVVM + +  +IDP GW EW  DFA+ TLFY E++N G GS T  RV WPG+HV+ + E
Sbjct: 469 RTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPE 528

Query: 451 EARPFTVAEFLHGGDWIPTTGVPFEAGV 458
            A  FTVAE + GG W+ +TGV + AG+
Sbjct: 529 VAEQFTVAELIQGGSWLGSTGVDYTAGL 543

BLAST of PI0010128 vs. ExPASy TrEMBL
Match: A0A0A0L3U7 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126790 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.0e-241
Identity = 424/487 (87.06%), Postives = 435/487 (89.32%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALD-----------------------------PAA 60
           MPFPFTTFLLLLFLP VASSNFSLPALD                              AA
Sbjct: 1   MPFPFTTFLLLLFLPFVASSNFSLPALDSVRLAQTGVREAITEIGNLKRRQFGDWNLQAA 60

Query: 61  LRDCSTLYEEAEWRLAGMLVGEN-YRAEDGRMWVSAAVANHRSCLDGLEEVGGGAAVDGN 120
           LRDC+TLYEEAEWRLAGMLVGEN YRAEDGRMWVSAA+ANHRSCLDGLEEV   AAVDGN
Sbjct: 61  LRDCATLYEEAEWRLAGMLVGENKYRAEDGRMWVSAAMANHRSCLDGLEEVHDVAAVDGN 120

Query: 121 NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAKDGS 180
           NLTVMLTGALHLYDKIAAVEKRNG KRL KRKWRENRG NLATWN ATSKA+YVVAKDGS
Sbjct: 121 NLTVMLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKDGS 180

Query: 181 GTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKTIVT 240
           GTHRTIN AVAALAR+GR RRGGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKTIVT
Sbjct: 181 GTHRTINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTIVT 240

Query: 241 GSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIK 300
           GSRNVPDGATTYNSATFGVSGDGFW RDITFENTAGPGK QAVALRLNSDLAVVYRCAIK
Sbjct: 241 GSRNVPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCAIK 300

Query: 301 GYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDD 360
           GYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQPI HQANMITAQGRDD
Sbjct: 301 GYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGRDD 360

Query: 361 PAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEW 420
           PAEATG SILNSRVQP P+F+PVK QYRTYLGRPWKRFSRTVV+ATDLDGLIDPKGWGEW
Sbjct: 361 PAEATGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWGEW 420

Query: 421 TGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTG 458
           TGDFAISTLFYAEFKN+GDGSSTRFRVNWPGFH+L + EEARPFTVAEFLHGGDWIP TG
Sbjct: 421 TGDFAISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPATG 480

BLAST of PI0010128 vs. ExPASy TrEMBL
Match: A0A5D3D3J9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003760 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.1e-238
Identity = 422/490 (86.12%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAVTEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTV+M TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVIMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEFKN+GDGSSTRFRVNWPGFHVL + EEARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSAEEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. ExPASy TrEMBL
Match: A0A1S3AVG1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 422/490 (86.12%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAVTEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTVVM TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEFKN+GDGSSTRFRVNWPGFHVL + +EARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. ExPASy TrEMBL
Match: A0A5A7U173 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002820 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.5e-237
Identity = 421/490 (85.92%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAISEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTVVM TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEF+N+GDGSSTRFRVNWPGFHVL + +EARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFENRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. ExPASy TrEMBL
Match: A0A6J1IAJ0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.4e-206
Identity = 368/486 (75.72%), Postives = 400/486 (82.30%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALD-----------------------------PAA 60
           MPFPF T  LLLFL ++ASS  SL   D                               A
Sbjct: 1   MPFPFAT-PLLLFLAIMASSISSLDEPDAVRLLRTGIVKARSEIYNVKRRRFERGNVQTA 60

Query: 61  LRDCSTLYEEAEWRLAGMLVGENYRAEDGRMWVSAAVANHRSCLDGLEEVGGGAAVDGNN 120
           L DC TLYEEA+ RLAGMLVGENY A+D R+W+SAAVANHRSCLDGLEEVG   + DGNN
Sbjct: 61  LGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGAVPSADGNN 120

Query: 121 LTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAKDGSG 180
           LT++LTGALHLYDKIAAVE+R  W+R+EK + REN  INLA WN ATSK DYVVAKDGSG
Sbjct: 121 LTMLLTGALHLYDKIAAVEERKCWERVEKERLRENGRINLAKWNAATSKPDYVVAKDGSG 180

Query: 181 THRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKTIVTG 240
           T+ TINGAVAALAR GRRRR  R+VIYVKAGVY+ENVEIG+Q KNVM+VGDG DKTIVTG
Sbjct: 181 TYTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDGADKTIVTG 240

Query: 241 SRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIKG 300
           +RNVPDGATTY+SATFGVSGDGFWARDITFENTAGPGK QAVALRLNSD  VVYRC IKG
Sbjct: 241 NRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPGKQQAVALRLNSDRVVVYRCTIKG 300

Query: 301 YQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDDP 360
           YQDTLFLHSLRQFYRDCQIYGTIDFIFGN+AAVLQNC+IFVR+PIRHQANMITAQGRDDP
Sbjct: 301 YQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANMITAQGRDDP 360

Query: 361 AEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEWT 420
           AEATGISI++ RV+  P+ A VKG +RTYLGRPWKRFSRTV+M TDLDGLIDPKGWGEW 
Sbjct: 361 AEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVIMTTDLDGLIDPKGWGEWR 420

Query: 421 GDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTGV 458
           GDFA+STLFYAEFKN G GSSTRFRV+WPGFHVL + EEAR FTVA FL G  WIP TGV
Sbjct: 421 GDFALSTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAGFLRGDAWIPATGV 480

BLAST of PI0010128 vs. NCBI nr
Match: KAE8650273.1 (hypothetical protein Csa_010781 [Cucumis sativus])

HSP 1 Score: 844.0 bits (2179), Expect = 6.2e-241
Identity = 424/487 (87.06%), Postives = 435/487 (89.32%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALD-----------------------------PAA 60
           MPFPFTTFLLLLFLP VASSNFSLPALD                              AA
Sbjct: 4   MPFPFTTFLLLLFLPFVASSNFSLPALDSVRLAQTGVREAITEIGNLKRRQFGDWNLQAA 63

Query: 61  LRDCSTLYEEAEWRLAGMLVGEN-YRAEDGRMWVSAAVANHRSCLDGLEEVGGGAAVDGN 120
           LRDC+TLYEEAEWRLAGMLVGEN YRAEDGRMWVSAA+ANHRSCLDGLEEV   AAVDGN
Sbjct: 64  LRDCATLYEEAEWRLAGMLVGENKYRAEDGRMWVSAAMANHRSCLDGLEEVHDVAAVDGN 123

Query: 121 NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAKDGS 180
           NLTVMLTGALHLYDKIAAVEKRNG KRL KRKWRENRG NLATWN ATSKA+YVVAKDGS
Sbjct: 124 NLTVMLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKDGS 183

Query: 181 GTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKTIVT 240
           GTHRTIN AVAALAR+GR RRGGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKTIVT
Sbjct: 184 GTHRTINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTIVT 243

Query: 241 GSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIK 300
           GSRNVPDGATTYNSATFGVSGDGFW RDITFENTAGPGK QAVALRLNSDLAVVYRCAIK
Sbjct: 244 GSRNVPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCAIK 303

Query: 301 GYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDD 360
           GYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQPI HQANMITAQGRDD
Sbjct: 304 GYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGRDD 363

Query: 361 PAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEW 420
           PAEATG SILNSRVQP P+F+PVK QYRTYLGRPWKRFSRTVV+ATDLDGLIDPKGWGEW
Sbjct: 364 PAEATGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWGEW 423

Query: 421 TGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTG 458
           TGDFAISTLFYAEFKN+GDGSSTRFRVNWPGFH+L + EEARPFTVAEFLHGGDWIP TG
Sbjct: 424 TGDFAISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPATG 483

BLAST of PI0010128 vs. NCBI nr
Match: XP_004134349.3 (probable pectinesterase/pectinesterase inhibitor 36 [Cucumis sativus])

HSP 1 Score: 844.0 bits (2179), Expect = 6.2e-241
Identity = 424/487 (87.06%), Postives = 435/487 (89.32%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALD-----------------------------PAA 60
           MPFPFTTFLLLLFLP VASSNFSLPALD                              AA
Sbjct: 1   MPFPFTTFLLLLFLPFVASSNFSLPALDSVRLAQTGVREAITEIGNLKRRQFGDWNLQAA 60

Query: 61  LRDCSTLYEEAEWRLAGMLVGEN-YRAEDGRMWVSAAVANHRSCLDGLEEVGGGAAVDGN 120
           LRDC+TLYEEAEWRLAGMLVGEN YRAEDGRMWVSAA+ANHRSCLDGLEEV   AAVDGN
Sbjct: 61  LRDCATLYEEAEWRLAGMLVGENKYRAEDGRMWVSAAMANHRSCLDGLEEVHDVAAVDGN 120

Query: 121 NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAKDGS 180
           NLTVMLTGALHLYDKIAAVEKRNG KRL KRKWRENRG NLATWN ATSKA+YVVAKDGS
Sbjct: 121 NLTVMLTGALHLYDKIAAVEKRNGRKRLGKRKWRENRGTNLATWNPATSKANYVVAKDGS 180

Query: 181 GTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKTIVT 240
           GTHRTIN AVAALAR+GR RRGGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKTIVT
Sbjct: 181 GTHRTINRAVAALARSGRTRRGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKTIVT 240

Query: 241 GSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIK 300
           GSRNVPDGATTYNSATFGVSGDGFW RDITFENTAGPGK QAVALRLNSDLAVVYRCAIK
Sbjct: 241 GSRNVPDGATTYNSATFGVSGDGFWVRDITFENTAGPGKQQAVALRLNSDLAVVYRCAIK 300

Query: 301 GYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDD 360
           GYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQPI HQANMITAQGRDD
Sbjct: 301 GYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPILHQANMITAQGRDD 360

Query: 361 PAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEW 420
           PAEATG SILNSRVQP P+F+PVK QYRTYLGRPWKRFSRTVV+ATDLDGLIDPKGWGEW
Sbjct: 361 PAEATGFSILNSRVQPAPEFSPVKAQYRTYLGRPWKRFSRTVVIATDLDGLIDPKGWGEW 420

Query: 421 TGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTG 458
           TGDFAISTLFYAEFKN+GDGSSTRFRVNWPGFH+L + EEARPFTVAEFLHGGDWIP TG
Sbjct: 421 TGDFAISTLFYAEFKNRGDGSSTRFRVNWPGFHILRSAEEARPFTVAEFLHGGDWIPATG 480

BLAST of PI0010128 vs. NCBI nr
Match: TYK17509.1 (putative pectinesterase/pectinesterase inhibitor 36 [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 1.9e-237
Identity = 422/490 (86.12%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAVTEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTV+M TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVIMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEFKN+GDGSSTRFRVNWPGFHVL + EEARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSAEEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. NCBI nr
Match: XP_008438191.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Cucumis melo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.2e-237
Identity = 422/490 (86.12%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAVTEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTVVM TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEFKN+GDGSSTRFRVNWPGFHVL + +EARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. NCBI nr
Match: KAA0049054.1 (putative pectinesterase/pectinesterase inhibitor 36 [Cucumis melo var. makuwa])

HSP 1 Score: 830.1 bits (2143), Expect = 9.3e-237
Identity = 421/490 (85.92%), Postives = 432/490 (88.16%), Query Frame = 0

Query: 1   MPFPFTTFLLLLFLPLVASSNFSLPALDP------------------------------A 60
           MPFPFTTFLLLL LP VASSNFSLPALD                               A
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREAISEIGNLKRRRFGDGNHLLA 60

Query: 61  ALRDCSTLYEEAEWRLAGMLVG--ENYRAEDGRMWVSAAVANHRSCLDGLEEV-GGGAAV 120
           ALRDC+ LYEEAEWRLAGMLVG  + Y AEDGRMWVSAA+ANHRSCLDGLEEV   GAAV
Sbjct: 61  ALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEVDADGAAV 120

Query: 121 DGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNQATSKADYVVAK 180
            G+NLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWN ATSKADYVVAK
Sbjct: 121 GGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSKADYVVAK 180

Query: 181 DGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKNVMLVGDGTDKT 240
           DGSGTH TIN AVAALA TGRRR GGRIVIYVKAGVYRENVEIGIQ KNVMLVGDG DKT
Sbjct: 181 DGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLVGDGIDKT 240

Query: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRC 300
           IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGP K QAVALRLNSDLAVVYRC
Sbjct: 241 IVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAVVYRC 300

Query: 301 AIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQG 360
           AIKGYQDTLFLHSLRQFYRDC+IYGTIDFIFGNSAAVLQNCEIFVRQP RHQANMITAQG
Sbjct: 301 AIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQANMITAQG 360

Query: 361 RDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGW 420
           RDDPAEATGISILNSRVQPTPDFAPVK QYRTYLGRPWKRFSRTVVM TDLDGLIDPKGW
Sbjct: 361 RDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDGLIDPKGW 420

Query: 421 GEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIP 458
           GEWTG+FAISTLFYAEF+N+GDGSSTRFRVNWPGFHVL + +EARPFTVAEFLHGGDWIP
Sbjct: 421 GEWTGNFAISTLFYAEFENRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFLHGGDWIP 480

BLAST of PI0010128 vs. TAIR 10
Match: AT3G60730.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 525.4 bits (1352), Expect = 4.6e-149
Identity = 261/452 (57.74%), Postives = 328/452 (72.57%), Query Frame = 0

Query: 32  LRDCSTLYEEAEWRLAGMLVG-ENYRAEDGRMWVSAAVANHRSCLDGL-EEVGGGAAVDG 91
           L +C  LY+E+E RL+ ++V  EN+  ED R W+S  +ANH +CLDGL ++  G   +  
Sbjct: 69  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 128

Query: 92  NNLTVMLTGALHLYDKIAAVEKR---------NGWKRLEKRKWRENRGIN---------- 151
           +N+T +L  AL  Y K     K+         +G  R + R  R N G            
Sbjct: 129 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 188

Query: 152 -----LATWNQATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYR 211
                L +WN  +S+AD+VVA+DGS THRTIN A+AA++R G+ R   R++IY+KAGVY 
Sbjct: 189 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRL-NRVIIYIKAGVYN 248

Query: 212 ENVEIGIQSKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTA 271
           E +EI    KN+MLVGDG D+TIVT +RNVPDG+TTY SATFGVSGDGFWARDITFENTA
Sbjct: 249 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTA 308

Query: 272 GPGKHQAVALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVL 331
           GP KHQAVALR++SDL++ YRC+ KGYQDTLF HSLRQFYRDC IYGTIDFIFG++AAV 
Sbjct: 309 GPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVF 368

Query: 332 QNCEIFVRQPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPW 391
           QNC+IFVR+P+ HQ NMITAQGRDDP   +GISI +SR++  P+F  VKG++++YLGRPW
Sbjct: 369 QNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPW 428

Query: 392 KRFSRTVVMATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVL 451
           K++SRTV + TD+D LIDP+GW EW+G +A+STL+Y EF N G G+ T  RVNWPGFHVL
Sbjct: 429 KKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVL 488

Query: 452 CTTEEARPFTVAEFLHGGDWIPTTGVPFEAGV 458
              EEA PFTV+ F+ G  WIP TGVPF AGV
Sbjct: 489 RGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

BLAST of PI0010128 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 388.7 bits (997), Expect = 6.7e-108
Identity = 219/441 (49.66%), Postives = 280/441 (63.49%), Query Frame = 0

Query: 28  DPAALRDCSTLYEEAEWRLAGMLVGENYRAE--DGRMWVSAAVANHRSCLDGLEEVGGGA 87
           + AA  DC  LY+    ++    +  N +    D + W+S A+ N  +C  G  E+G   
Sbjct: 93  EKAAWEDCIKLYDLTVSKI-NETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTD 152

Query: 88  AV---DGNNLTVMLTGALHLYDKIA---AVEKRNG---WKRLEKRKWRENRGINLATWNQ 147
            V     NN++ +L   L + +K+       +++G   W +   RK  ++          
Sbjct: 153 IVLPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPGDRKLLQS---------- 212

Query: 148 ATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQSKN 207
           +T K + VVAKDGSG  +TI  A+ A + +      GR VIYVK GVY EN+E  I+ KN
Sbjct: 213 STPKDNAVVAKDGSGNFKTIKEAIDAASGS------GRFVIYVKQGVYSENLE--IRKKN 272

Query: 208 VMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVALR 267
           VML GDG  KTI+TGS++V  G TT+NSAT    GDGF AR ITF NTAG    QAVALR
Sbjct: 273 VMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALR 332

Query: 268 LNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQPI 327
             SDL+V Y+C+ + YQDTL++HS RQFYRDC +YGT+DFIFGN+AAVLQNC IF R+P 
Sbjct: 333 SGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP- 392

Query: 328 RHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVMAT 387
           R + N ITAQGR DP + TGI I NSRV    D  PV G  +TYLGRPW+++SRTV M T
Sbjct: 393 RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKT 452

Query: 388 DLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPFTV 447
            LD LIDP+GW EW G+FA+ TLFYAEF+N G G+ST  RV WPGF VL +  EA  FTV
Sbjct: 453 SLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTV 511

Query: 448 AEFLHGGDWIPTTGVPFEAGV 458
             FL GG WIP++ VPF +G+
Sbjct: 513 GTFLAGGSWIPSS-VPFTSGL 511

BLAST of PI0010128 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 388.7 bits (997), Expect = 6.7e-108
Identity = 211/446 (47.31%), Postives = 270/446 (60.54%), Query Frame = 0

Query: 30  AALRDCSTLYEEAEWRLAGMLVGENYRAE--------DGRMWVSAAVANHRSCLDGLEEV 89
           A L DC  LY +   +L   L G + +A         D + W+S A+ N  +C  G  ++
Sbjct: 99  AVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDL 158

Query: 90  GGGAAVDGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRG-INLATWNQATSK 149
                 D     V  T   HL     AV   NG       K           TW     K
Sbjct: 159 N---VTDFITPIVSNTKISHLISNCLAV---NGALLTAGNKGNTTANQKGFPTWLSRKDK 218

Query: 150 -------ADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQ 209
                  A+ VVAKDGSG   T+  A+    R  R+   GR VIYVK G+Y+EN+ + + 
Sbjct: 219 RLLRAVRANLVVAKDGSGHFNTVQAAIDVAGR--RKVTSGRFVIYVKRGIYQENINVRLN 278

Query: 210 SKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAV 269
           + ++MLVGDG   TI+TG R+V  G TTYNSAT G+ G  F A+ ITF NTAGP K QAV
Sbjct: 279 NDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAV 338

Query: 270 ALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVR 329
           ALR +SDL++ Y+C+I+GYQDTL +HS RQFYR+C IYGT+DFIFGN+AAV QNC I  R
Sbjct: 339 ALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPR 398

Query: 330 QPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVV 389
           +P++ QAN+ITAQGR DP + TGISI NSR+ P PD  PV G  +TY+GRPW +FSRTVV
Sbjct: 399 RPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVV 458

Query: 390 MATDLDGLIDPKGWGEWT--GDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEA 449
           + T LD ++ P GW  W     F + TLFYAE+KN G  SSTR+RV+W GFHVL    +A
Sbjct: 459 LQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDA 518

Query: 450 RPFTVAEFLHGGDWIPTTGVPFEAGV 458
             FTV +F+ G  W+P TG+PF +G+
Sbjct: 519 SAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of PI0010128 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 377.9 bits (969), Expect = 1.2e-104
Identity = 203/466 (43.56%), Postives = 288/466 (61.80%), Query Frame = 0

Query: 16  LVASSNFSLPALDP---AALRDCSTLYE---EAEWRLAGMLVGENYRAEDGRMWVSAAVA 75
           L +S++ S   + P   +A   C  L +   +A  R    +V  + + +D   W+SAA+ 
Sbjct: 135 LYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALT 194

Query: 76  NHRSCLDGLEEVGGGAAVDG-----NNLTVMLTGALHLY------DKIAAVEKRN----G 135
           NH +C +G + V  G   D       NL+ +++  L ++      D  A V  +N    G
Sbjct: 195 NHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLG 254

Query: 136 WKRLEKR--KWRENRGINLATWNQATSKADYVVAKDGSGTHRTINGAVAALARTGRRRRG 195
            +  E++  +W   +   +     +  +AD +V+KDG+GT +TI+ A+    +   R   
Sbjct: 255 VEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR--- 314

Query: 196 GRIVIYVKAGVYRE-NVEIGIQSKNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSG 255
            RI+IYVKAG Y E N+++G +  N+M VGDG  KT+++G +++ D  TT+++A+F  +G
Sbjct: 315 -RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATG 374

Query: 256 DGFWARDITFENTAGPGKHQAVALRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIY 315
            GF ARDITFEN AGP KHQAVALR+ +D AV+YRC I GYQDTL++HS RQF+R+C IY
Sbjct: 375 AGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIY 434

Query: 316 GTIDFIFGNSAAVLQNCEIFVRQPIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFA 375
           GT+DFIFGN+A VLQNC I+ R+P+  Q N ITAQ R DP + TGISI  SRV    D  
Sbjct: 435 GTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQ 494

Query: 376 PVKGQYRTYLGRPWKRFSRTVVMATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGS 435
              G  +TYLGRPWK FSRTV M + + G +  +GW EW   FA+ TL+Y E+ N G GS
Sbjct: 495 ATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGS 554

Query: 436 STRFRVNWPGFHVLCTTEEARPFTVAEFLHGGDWIPTTGVPFEAGV 458
               RV+WPG+ V+ +T EA  FTVAEF++G  W+P+TGV F AG+
Sbjct: 555 GLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

BLAST of PI0010128 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 375.2 bits (962), Expect = 7.6e-104
Identity = 200/440 (45.45%), Postives = 272/440 (61.82%), Query Frame = 0

Query: 28  DPAALRDCSTLYEEAEWRLAGM---LVGENYRAE---DGRMWVSAAVANHRSCLDGLEEV 87
           D A   DC  + + ++ R+      L G NY  E   +   W+S+ + N+ +CL+ + +V
Sbjct: 119 DKAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDV 178

Query: 88  GGGA--AVDGNNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRK---WRENRGINLATWNQ 147
              +   V      ++    + L   ++ +  R+  K +   +   W       L   + 
Sbjct: 179 SVNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSP 238

Query: 148 ATSK--ADYVVAKDGSGTHRTINGAVAALARTGRRRRGGRIVIYVKAGVYRENVEIGIQS 207
            T K  A+ VVAKDG+G  +T+N AVAA           R VIYVK GVY+E ++IG + 
Sbjct: 239 KTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNT----RYVIYVKKGVYKETIDIGKKK 298

Query: 208 KNVMLVGDGTDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPGKHQAVA 267
           KN+MLVGDG D TI+TGS NV DG+TT+ SAT   +GDGF A+DI F+NTAGP KHQAVA
Sbjct: 299 KNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVA 358

Query: 268 LRLNSDLAVVYRCAIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNSAAVLQNCEIFVRQ 327
           LR+++D  V+ RC I  YQDTL+ H+LRQFYRD  I GT+DFIFGNSA V QNC+I  R 
Sbjct: 359 LRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARN 418

Query: 328 PIRHQANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKGQYRTYLGRPWKRFSRTVVM 387
           P   Q NM+TAQGR+D  + T ISI   ++  + D APVKG  +T+LGRPWK +SRTV+M
Sbjct: 419 PGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIM 478

Query: 388 ATDLDGLIDPKGWGEWTGDFAISTLFYAEFKNKGDGSSTRFRVNWPGFHVLCTTEEARPF 447
            + +D  IDP GW  W G+FA+STL+Y E+ N G G+ T  RVNW GF V+  ++EA  F
Sbjct: 479 QSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQF 538

Query: 448 TVAEFLHGGDWIPTTGVPFE 455
           TVA+ + GG W+  TGV F+
Sbjct: 539 TVAKLIQGGLWLKPTGVTFQ 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84R106.4e-14857.74Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3... [more]
O048871.5e-10948.63Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221499.4e-10749.66Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN59.4e-10747.31Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
P839471.1e-10445.54Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423... [more]
Match NameE-valueIdentityDescription
A0A0A0L3U73.0e-24187.06Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126790 PE=3 SV=1[more]
A0A5D3D3J99.1e-23886.12Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00376... [more]
A0A1S3AVG11.6e-23786.12Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1[more]
A0A5A7U1734.5e-23785.92Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00282... [more]
A0A6J1IAJ01.4e-20675.72Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAE8650273.16.2e-24187.06hypothetical protein Csa_010781 [Cucumis sativus][more]
XP_004134349.36.2e-24187.06probable pectinesterase/pectinesterase inhibitor 36 [Cucumis sativus][more]
TYK17509.11.9e-23786.12putative pectinesterase/pectinesterase inhibitor 36 [Cucumis melo var. makuwa][more]
XP_008438191.13.2e-23786.12PREDICTED: probable pectinesterase/pectinesterase inhibitor 36 [Cucumis melo][more]
KAA0049054.19.3e-23785.92putative pectinesterase/pectinesterase inhibitor 36 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G60730.14.6e-14957.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.16.7e-10849.66Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.16.7e-10847.31Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G49220.11.2e-10443.56Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G11580.17.6e-10445.45methylesterase PCR A [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..84
e-value: 5.2E-7
score: 30.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 17..108
e-value: 1.8E-6
score: 29.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 27..83
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 136..457
e-value: 2.0E-116
score: 390.9
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 142..442
e-value: 1.1E-135
score: 451.2
NoneNo IPR availablePANTHERPTHR31707:SF186PECTINESTERASE/PECTINESTERASE INHIBITOR 36-RELATEDcoord: 36..456
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 36..456
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 290..299
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 137..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010128.1PI0010128.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity