PI0009967 (gene) Melon (PI 482460) v1

Overview
NamePI0009967
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionNudix hydrolase
Locationchr10: 20978743 .. 20983769 (-)
RNA-Seq ExpressionPI0009967
SyntenyPI0009967
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATCTTCAATAAATGTATATAAGTTTTCTCAATACGAAAAAAAATGAAAATAAAATATTTCGAGAGAGAAGAATAAATAAAGCGCGAAGCGTTGGAAGAACGCTCCAAAGAAATGGCCTTAAACCGATATGTCGTTTTTAGTCATTCACTTTTTCACTATCGTCGTCCTCCTCCTCCTCCTCCTCCATTTTTCTCATCTGCTCTGCGTTCGACCTCCTCGTCCATGGAAGCTCCTCCCGCAGGTTACCGCAAAAACGTTGGGATTTGCCTCATCAATCCGTCTAAAAAGGTCATTCTCGAAATCTTTCTCTTTATATTCCTCCATCTCTGCTCATCTCTACAAGTATTCATCCATGCGCGTCGTTCCAATATCGGTATTTGCAGATTTTTGCTGCCTCAAGGTTGGATATACCAGATGCTTGGCAAATGCCTCAGGTAATTGGATGATTTTTTTTTCTCTTGCTTCTAGGTTGTGATTGTTTTTTATCCGGAAGTTCGATGTACAAGCAGCATATTTTTAGCTCTGAATAATGAATTGCTACTTGCTCTAGGGCTTCATTGTTCAATTTTCGCGTATTAGGCTTTATGAAGAGTTTATTCGATCAGATGTATTCATTAAGTCTGAAATTCTAGCGTTGAAGCATAGTTAAGTGGCAAGGATATAATGTATTATCATCTTCGTATTTTCTTGAGTGTTTTTATATGTTTTAGCTATCACGAGTTATTTTTGTCGTGTTGTGTTCATAGGGCGGCGTGGACGAGGGTGAAGATCCAAGAAGTGCAGCCATTAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACTATTTCATTGATGCATTTGGTTGTCTTTGCTTGAATATGCATATTCTTCTCTCAAGAGTTCATTAATCAAGTGCTTTTCGAACTCGTTAAAACTGAAGAAAGTCTATTTTAGGAGTATAGAGGACTAGATTTTTGCTGTTCAAATGAATTAGATCTCGATGGCATCCAGTTTCAGTTGCAGTTAATATGATGGTACAAAAGAAAGTTCATAACTGATCACCACTGTATCGACCGAGGTCTTTGAGTGAGTTTAGGGTGTATTTGATGCTTTTCTTGTTCTCAACTTTACCATCAAATTTCTAAACATCAATTTTTAAAGAGAGATGAGTTTGAAGCATGACTCTAATTTCTGTGCTAACCCGTCGTAACCCATGTGGTCATAATTTTCTTTAACCTCTTGTGATGGAGGAAGAATATTATGAACTTTACCAGCGATTTTCCTGCCATTGACTTGGCCTTAAAATTCTTCTACCACTATGGCCTGTTTCTTTTATGACCCTCTGCAGTATCATTGCTGCACTATAGGTTATGATGTATTAGTAGTTTTAATATGCATATTGTGTATGTTACTGAAATTGTGAATTCGCCTTATATTTTGCTTCTTTCATTTGGGAGTTTCTCCAGAGTAAAAGTCTGTGCTTACCTTATTTGGACACATCAATGAAATGTCTTGAATATTGGTTTCTTAAAGTAGGTACCTTATTGGGTGACTTATGACTTCCCACCACATGTTCGGGAAAAACTTAGGCAACAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAATGGTGAGTTCTATTTTCTATGCAAACAGTTCTAATTTCTTTCATATTCTTTAGAACTAAATCTCACTTGATGTATACTGGTTCACATAGTTATTAATACCTCAATGCTGATCCTTTTTTTAAAAAAGGGTAAACAACCTGTGTGCTAAAACTCTGTTTCCTTATTACCTGGAAAGCCGGTGCACCATACCTAAGTTACTCAGATCAGGGAGCAAAGCTATGAATTTTTGTGAGGGGTCTTAGAAATCATATAAGGGACATTCCTTTCCTAACGATATGTGTATTAAAAGAACCAAAATCCACCCTCAATGAATTTGCATGGATGACAGTGTTAGTATTTTGAGTCTTCATTGGTTTTTCTGTTAAAACTTTTGCAAGTAACTTTTTTTCTTTCGTTTTTTTTTTTCATCCTGTCAAGGCAGTATTTCATTCCCTTACCAACTAAAATTTAATGATAGTTTTCTATTCTATCTGCTGCTCTTACATCACGTTCAAATGTTTGTAAAAATTTAGGTTTCTGCTGAAGCTGACAGGAAGTGATGAAGAGATCAATCTTCTAGGTGATGGGACTGAGAAAGCCGAGTTTGGCGAATGGTCATGGATGTCACCTGAACAAGTAGTAGAATGTGTGAGTAGACCACCATTATCCTTCTTTATTTAATTCAACAATTGTAAGGTGGGATCGAACCATTAATCTTTGAAATAAGTTATTAATACCTTATCAAATTGACAAACCGCCATTTAACTGTTTAACACTCATCGCCAACCAAAATAAGCAGAGATGATCAGAAAAACGAATATGGATATACAACTTACATTTACCCTCTATCATGCAGGCCGTAGATTTCAAGAAACCTGTATACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAATATTATTCTGTTTCACAAACATTTATTATTGTCCCTTTCAATAATGTTGGTTTTACTTTTGTCCAATGAACTAAGTTAATATTTATAGCGGCGATATATCAAACCAGCTTGATTCGGGGAATTAGTTCTCTTATTTATTTATTTATAAAACGTCAATAAAACTTTGTTTGTGGGATA

mRNA sequence

ATTATCTTCAATAAATGTATATAAGTTTTCTCAATACGAAAAAAAATGAAAATAAAATATTTCGAGAGAGAAGAATAAATAAAGCGCGAAGCGTTGGAAGAACGCTCCAAAGAAATGGCCTTAAACCGATATGTCGTTTTTAGTCATTCACTTTTTCACTATCGTCGTCCTCCTCCTCCTCCTCCTCCATTTTTCTCATCTGCTCTGCGTTCGACCTCCTCGTCCATGGAAGCTCCTCCCGCAGGTTACCGCAAAAACGTTGGGATTTGCCTCATCAATCCGTCTAAAAAGATTTTTGCTGCCTCAAGGTTGGATATACCAGATGCTTGGCAAATGCCTCAGGGCGGCGTGGACGAGGGTGAAGATCCAAGAAGTGCAGCCATTAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACCTTATTGGGTGACTTATGACTTCCCACCACATGTTCGGGAAAAACTTAGGCAACAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAATGGTTTCTGCTGAAGCTGACAGGAAGTGATGAAGAGATCAATCTTCTAGGTGATGGGACTGAGAAAGCCGAGTTTGGCGAATGGTCATGGATGTCACCTGAACAAGTAGTAGAATGTGCCGTAGATTTCAAGAAACCTGTATACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAATATTATTCTGTTTCACAAACATTTATTATTGTCCCTTTCAATAATGTTGGTTTTACTTTTGTCCAATGAACTAAGTTAATATTTATAGCGGCGATATATCAAACCAGCTTGATTCGGGGAATTAGTTCTCTTATTTATTTATTTATAAAACGTCAATAAAACTTTGTTTGTGGGATA

Coding sequence (CDS)

ATGGCCTTAAACCGATATGTCGTTTTTAGTCATTCACTTTTTCACTATCGTCGTCCTCCTCCTCCTCCTCCTCCATTTTTCTCATCTGCTCTGCGTTCGACCTCCTCGTCCATGGAAGCTCCTCCCGCAGGTTACCGCAAAAACGTTGGGATTTGCCTCATCAATCCGTCTAAAAAGATTTTTGCTGCCTCAAGGTTGGATATACCAGATGCTTGGCAAATGCCTCAGGGCGGCGTGGACGAGGGTGAAGATCCAAGAAGTGCAGCCATTAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACCTTATTGGGTGACTTATGACTTCCCACCACATGTTCGGGAAAAACTTAGGCAACAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAATGGTTTCTGCTGAAGCTGACAGGAAGTGATGAAGAGATCAATCTTCTAGGTGATGGGACTGAGAAAGCCGAGTTTGGCGAATGGTCATGGATGTCACCTGAACAAGTAGTAGAATGTGCCGTAGATTTCAAGAAACCTGTATACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAA

Protein sequence

MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPHLE
Homology
BLAST of PI0009967 vs. ExPASy Swiss-Prot
Match: Q9CAF2 (Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.8e-76
Identity = 129/171 (75.44%), Postives = 148/171 (86.55%), Query Frame = 0

Query: 28  SSALRSTSSSMEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRS 87
           SS+     SSME+PP GYR+NVG+CL+N SKKIF ASRLDIP AWQMPQGG+DEGEDPR 
Sbjct: 46  SSSSARCCSSMESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMPQGGIDEGEDPRV 105

Query: 88  AAIRELREETGVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGS 147
           A +REL+EETGV SAEI+AE P+W+TYDFPP VREKL+ +WGSDWKGQAQKWFLLK TG 
Sbjct: 106 AVMRELKEETGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWFLLKFTGK 165

Query: 148 DEEINLLGDGTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPHLE 199
           DEEINLLGDGTEK EFGEWSW SP+QVVE AV+FKKPVYK+V++ F  HL+
Sbjct: 166 DEEINLLGDGTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFASHLQ 216

BLAST of PI0009967 vs. ExPASy Swiss-Prot
Match: Q9FNH4 (Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 7.3e-64
Identity = 113/176 (64.20%), Postives = 137/176 (77.84%), Query Frame = 0

Query: 21  PPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVD 80
           P P    S AL S + ++E+PP GYRKNVGICL++P +KIF AS++ IPD WQMPQGG D
Sbjct: 38  PKPLVVLSVALSSPARTVESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGGAD 97

Query: 81  EGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWF 140
           EGED R+AA RELREETGV SAE IAE+P W+TYDFP  V++KL ++W + +KGQAQKWF
Sbjct: 98  EGEDLRNAAFRELREETGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWF 157

Query: 141 LLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPH 197
           L K TG +EEINLLGDGT K EF  WSWM PEQV+E AV FK+PVY+ V+  F P+
Sbjct: 158 LFKFTGKEEEINLLGDGTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

BLAST of PI0009967 vs. ExPASy Swiss-Prot
Match: Q9C6Z2 (Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.7e-52
Identity = 89/157 (56.69%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 38  MEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREET 97
           ME  P GYR NVG+CLIN    +F ASRL++P AWQMPQGG+++GEDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 98  GVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 157
           GV SAEI++EVP W+TYDFPP V+ K+ + WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 158 GTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVF 194
               +EF EW W  PE+VVE AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of PI0009967 vs. ExPASy Swiss-Prot
Match: Q3IZC1 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=rppH PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-39
Identity = 82/156 (52.56%), Postives = 103/156 (66.03%), Query Frame = 0

Query: 45  YRKNVGICLINPSKKIFAASRLDIP-DAWQMPQGGVDEGEDPRSAAIRELREETGV--RS 104
           YR  VGI LIN    IFA  R+D P  AWQMPQGG+DEGE PR AA+REL EETG+    
Sbjct: 12  YRPCVGIVLINREGLIFAGQRIDSPVPAWQMPQGGIDEGEKPREAALRELWEETGIPAER 71

Query: 105 AEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKA 164
            E +A+ P WVTYD PP   E L + WG  ++GQ QKWFL +  G+DEE+   G GT+ A
Sbjct: 72  VEFVAKAPDWVTYDLPP---ELLGRVWGGKYRGQRQKWFLYRYLGTDEEV---GIGTDHA 131

Query: 165 EFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPHL 198
           EF  W W+  E++V   V FK+ VY++V+  F+PHL
Sbjct: 132 EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of PI0009967 vs. ExPASy Swiss-Prot
Match: B9KN59 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain KD131 / KCTC 12085) OX=557760 GN=rppH PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-39
Identity = 82/156 (52.56%), Postives = 103/156 (66.03%), Query Frame = 0

Query: 45  YRKNVGICLINPSKKIFAASRLDIP-DAWQMPQGGVDEGEDPRSAAIRELREETGV--RS 104
           YR  VGI LIN    IFA  R+D P  AWQMPQGG+DEGE PR AA+REL EETG+    
Sbjct: 12  YRPCVGIVLINREGLIFAGQRIDSPVPAWQMPQGGIDEGEKPREAALRELWEETGIPAER 71

Query: 105 AEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKA 164
            E +A+ P WVTYD PP   E L + WG  ++GQ QKWFL +  G+DEE+   G GT+ A
Sbjct: 72  VEFVAKAPDWVTYDLPP---ELLGRVWGGKYRGQRQKWFLYRYLGTDEEV---GIGTDHA 131

Query: 165 EFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPHL 198
           EF  W W+  E++V   V FK+ VY++V+  F+PHL
Sbjct: 132 EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of PI0009967 vs. ExPASy TrEMBL
Match: A0A1S3BGM3 (nudix hydrolase 26, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489378 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.3e-105
Identity = 189/198 (95.45%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MALNRYVVFSHSLFHYRR    PPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI
Sbjct: 1   MALNRYVVFSHSLFHYRR----PPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGV+SA+IIAEVPYWVTYDFPP V
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFPPQV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPE+VVE AVD
Sbjct: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 194

BLAST of PI0009967 vs. ExPASy TrEMBL
Match: A0A0A0KRB9 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G613530 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 6.8e-105
Identity = 188/198 (94.95%), Postives = 190/198 (95.96%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MALNR VV SHSLFHYRR  PPPPPFFSSALRSTSSSMEAPP GYRKNVGICLINPSKKI
Sbjct: 1   MALNRCVVLSHSLFHYRR--PPPPPFFSSALRSTSSSMEAPPEGYRKNVGICLINPSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGV+SA+IIAEVPYWVTYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPE VVE AVD
Sbjct: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEDVVESAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 196

BLAST of PI0009967 vs. ExPASy TrEMBL
Match: A0A6J1HWY8 (nudix hydrolase 26, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467610 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.5e-99
Identity = 178/198 (89.90%), Postives = 185/198 (93.43%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MA++R VVF+HSL HY R    PPP FS+ALRSTSSSMEAPP GYR+NVGICL+N SKKI
Sbjct: 1   MAISRCVVFNHSLLHYHR---RPPPLFSTALRSTSSSMEAPPEGYRRNVGICLVNSSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYW+TYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPEQVVE AVD
Sbjct: 121 REKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 195

BLAST of PI0009967 vs. ExPASy TrEMBL
Match: A0A6J1FZW7 (nudix hydrolase 26, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449436 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.6e-98
Identity = 179/198 (90.40%), Postives = 184/198 (92.93%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MA++R VVF+HSL HY R    PP  FSSALRSTSSSMEAPP GYR+NVGICLIN SKKI
Sbjct: 17  MAISRCVVFNHSLLHYHR---RPPSLFSSALRSTSSSMEAPPEGYRRNVGICLINSSKKI 76

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYW+TYDFPPHV
Sbjct: 77  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHV 136

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPEQVVE AVD
Sbjct: 137 REKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVERAVD 196

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 197 FKKPVYKDVLTVFKPHLE 211

BLAST of PI0009967 vs. ExPASy TrEMBL
Match: A0A6J1DAT2 (nudix hydrolase 26, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018605 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.7e-93
Identity = 173/206 (83.98%), Postives = 182/206 (88.35%), Query Frame = 0

Query: 1   MALNRYVVFSHS-LFHYRRP-------PPPPPPFFSSALRSTSSSMEAPPAGYRKNVGIC 60
           MALNR VVF+HS L H RRP       P  P   FSSA R  SSSMEAPP GYR+NVGIC
Sbjct: 10  MALNRPVVFTHSVLLHCRRPLRLRLRLPTSPAALFSSAARPASSSMEAPPEGYRRNVGIC 69

Query: 61  LINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWV 120
           LIN SKKIFAASRLDIP+AWQMPQGGVDEGEDPR+AAIRELREETGVRSAEIIAEVPYW+
Sbjct: 70  LINSSKKIFAASRLDIPEAWQMPQGGVDEGEDPRNAAIRELREETGVRSAEIIAEVPYWL 129

Query: 121 TYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPE 180
           TYDFPPHVREKLR QWGSDWKGQAQKWFLLKLTG++EEINLLGDGTEKAEFGEWSWMSPE
Sbjct: 130 TYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGTEEEINLLGDGTEKAEFGEWSWMSPE 189

Query: 181 QVVECAVDFKKPVYKDVLTVFKPHLE 199
           QV+E AVDFKKPVYKDVL VFKP+LE
Sbjct: 190 QVLERAVDFKKPVYKDVLMVFKPYLE 215

BLAST of PI0009967 vs. NCBI nr
Match: XP_008446748.1 (PREDICTED: nudix hydrolase 26, chloroplastic [Cucumis melo])

HSP 1 Score: 391.3 bits (1004), Expect = 4.8e-105
Identity = 189/198 (95.45%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MALNRYVVFSHSLFHYRR    PPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI
Sbjct: 1   MALNRYVVFSHSLFHYRR----PPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGV+SA+IIAEVPYWVTYDFPP V
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFPPQV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPE+VVE AVD
Sbjct: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 194

BLAST of PI0009967 vs. NCBI nr
Match: XP_004135042.1 (nudix hydrolase 26, chloroplastic [Cucumis sativus] >KGN52175.1 hypothetical protein Csa_009002 [Cucumis sativus])

HSP 1 Score: 389.8 bits (1000), Expect = 1.4e-104
Identity = 188/198 (94.95%), Postives = 190/198 (95.96%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MALNR VV SHSLFHYRR  PPPPPFFSSALRSTSSSMEAPP GYRKNVGICLINPSKKI
Sbjct: 1   MALNRCVVLSHSLFHYRR--PPPPPFFSSALRSTSSSMEAPPEGYRKNVGICLINPSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGV+SA+IIAEVPYWVTYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPE VVE AVD
Sbjct: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEDVVESAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 196

BLAST of PI0009967 vs. NCBI nr
Match: XP_022968345.1 (nudix hydrolase 26, chloroplastic [Cucurbita maxima])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 178/198 (89.90%), Postives = 185/198 (93.43%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MA++R VVF+HSL HY R    PPP FS+ALRSTSSSMEAPP GYR+NVGICL+N SKKI
Sbjct: 1   MAISRCVVFNHSLLHYHR---RPPPLFSTALRSTSSSMEAPPEGYRRNVGICLVNSSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYW+TYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPEQVVE AVD
Sbjct: 121 REKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 195

BLAST of PI0009967 vs. NCBI nr
Match: KAG6573892.1 (Nudix hydrolase 26, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 179/198 (90.40%), Postives = 184/198 (92.93%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MA++R VVF+HSL HY R    PPP FSSALRSTSSSMEAPP GYR+NVGICLIN SKKI
Sbjct: 1   MAISRCVVFNHSLLHYHR---RPPPLFSSALRSTSSSMEAPPEGYRRNVGICLINSSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDE EDPRSAAIRELREETGVRSAEIIAEVPYW+TYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDESEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPEQVVE AVD
Sbjct: 121 REKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 195

BLAST of PI0009967 vs. NCBI nr
Match: XP_023541253.1 (nudix hydrolase 26, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.6 bits (945), Expect = 3.3e-98
Identity = 179/198 (90.40%), Postives = 184/198 (92.93%), Query Frame = 0

Query: 1   MALNRYVVFSHSLFHYRRPPPPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKI 60
           MA++R VVF+HSL HY R    PP  FSSALRSTSSSMEAPP GYR+NVGICLIN SKKI
Sbjct: 1   MAISRCVVFNHSLLHYHR---RPPSLFSSALRSTSSSMEAPPEGYRRNVGICLINSSKKI 60

Query: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHV 120
           FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYW+TYDFPPHV
Sbjct: 61  FAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHV 120

Query: 121 REKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVD 180
           REKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPEQVVE AVD
Sbjct: 121 REKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVERAVD 180

Query: 181 FKKPVYKDVLTVFKPHLE 199
           FKKPVYKDVLTVFKPHLE
Sbjct: 181 FKKPVYKDVLTVFKPHLE 195

BLAST of PI0009967 vs. TAIR 10
Match: AT3G10620.1 (nudix hydrolase homolog 26 )

HSP 1 Score: 286.2 bits (731), Expect = 2.0e-77
Identity = 129/171 (75.44%), Postives = 148/171 (86.55%), Query Frame = 0

Query: 28  SSALRSTSSSMEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRS 87
           SS+     SSME+PP GYR+NVG+CL+N SKKIF ASRLDIP AWQMPQGG+DEGEDPR 
Sbjct: 46  SSSSARCCSSMESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMPQGGIDEGEDPRV 105

Query: 88  AAIRELREETGVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGS 147
           A +REL+EETGV SAEI+AE P+W+TYDFPP VREKL+ +WGSDWKGQAQKWFLLK TG 
Sbjct: 106 AVMRELKEETGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWFLLKFTGK 165

Query: 148 DEEINLLGDGTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPHLE 199
           DEEINLLGDGTEK EFGEWSW SP+QVVE AV+FKKPVYK+V++ F  HL+
Sbjct: 166 DEEINLLGDGTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFASHLQ 216

BLAST of PI0009967 vs. TAIR 10
Match: AT5G06340.1 (nudix hydrolase homolog 27 )

HSP 1 Score: 245.0 bits (624), Expect = 5.2e-65
Identity = 113/176 (64.20%), Postives = 137/176 (77.84%), Query Frame = 0

Query: 21  PPPPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVD 80
           P P    S AL S + ++E+PP GYRKNVGICL++P +KIF AS++ IPD WQMPQGG D
Sbjct: 38  PKPLVVLSVALSSPARTVESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGGAD 97

Query: 81  EGEDPRSAAIRELREETGVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWF 140
           EGED R+AA RELREETGV SAE IAE+P W+TYDFP  V++KL ++W + +KGQAQKWF
Sbjct: 98  EGEDLRNAAFRELREETGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWF 157

Query: 141 LLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVFKPH 197
           L K TG +EEINLLGDGT K EF  WSWM PEQV+E AV FK+PVY+ V+  F P+
Sbjct: 158 LFKFTGKEEEINLLGDGTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

BLAST of PI0009967 vs. TAIR 10
Match: AT1G30110.1 (nudix hydrolase homolog 25 )

HSP 1 Score: 206.1 bits (523), Expect = 2.7e-53
Identity = 89/157 (56.69%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 38  MEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREET 97
           ME  P GYR NVG+CLIN    +F ASRL++P AWQMPQGG+++GEDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 98  GVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 157
           GV SAEI++EVP W+TYDFPP V+ K+ + WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 158 GTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVF 194
               +EF EW W  PE+VVE AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of PI0009967 vs. TAIR 10
Match: AT1G30110.2 (nudix hydrolase homolog 25 )

HSP 1 Score: 206.1 bits (523), Expect = 2.7e-53
Identity = 89/157 (56.69%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 38  MEAPPAGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREET 97
           ME  P GYR NVG+CLIN    +F ASRL++P AWQMPQGG+++GEDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 98  GVRSAEIIAEVPYWVTYDFPPHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 157
           GV SAEI++EVP W+TYDFPP V+ K+ + WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 158 GTEKAEFGEWSWMSPEQVVECAVDFKKPVYKDVLTVF 194
               +EF EW W  PE+VVE AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of PI0009967 vs. TAIR 10
Match: AT4G25434.2 (nudix hydrolase homolog 10 )

HSP 1 Score: 47.0 bits (110), Expect = 2.1e-05
Identity = 27/58 (46.55%), Postives = 38/58 (65.52%), Query Frame = 0

Query: 43  AGYRKNVGICLINPSKKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVR 101
           A +R  VG  ++N +K+    S L     W++P G VDEGE+  +AAIRE++EETGVR
Sbjct: 109 ASHRVRVGAVVLNHNKEEKYGS-LCGSGIWKIPTGVVDEGEEIFAAAIREVKEETGVR 165

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CAF22.8e-7675.44Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1... [more]
Q9FNH47.3e-6464.20Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1... [more]
Q9C6Z23.7e-5256.69Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1[more]
Q3IZC12.1e-3952.56RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158... [more]
B9KN592.1e-3952.56RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain KD131 / KCTC 12085) ... [more]
Match NameE-valueIdentityDescription
A0A1S3BGM32.3e-10595.45nudix hydrolase 26, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489378 PE=3 S... [more]
A0A0A0KRB96.8e-10594.95Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G61... [more]
A0A6J1HWY85.5e-9989.90nudix hydrolase 26, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467610 PE... [more]
A0A6J1FZW71.6e-9890.40nudix hydrolase 26, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1DAT27.7e-9383.98nudix hydrolase 26, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018605... [more]
Match NameE-valueIdentityDescription
XP_008446748.14.8e-10595.45PREDICTED: nudix hydrolase 26, chloroplastic [Cucumis melo][more]
XP_004135042.11.4e-10494.95nudix hydrolase 26, chloroplastic [Cucumis sativus] >KGN52175.1 hypothetical pro... [more]
XP_022968345.11.1e-9889.90nudix hydrolase 26, chloroplastic [Cucurbita maxima][more]
KAG6573892.12.0e-9890.40Nudix hydrolase 26, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023541253.13.3e-9890.40nudix hydrolase 26, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G10620.12.0e-7775.44nudix hydrolase homolog 26 [more]
AT5G06340.15.2e-6564.20nudix hydrolase homolog 27 [more]
AT1G30110.12.7e-5356.69nudix hydrolase homolog 25 [more]
AT1G30110.22.7e-5356.69nudix hydrolase homolog 25 [more]
AT4G25434.22.1e-0546.55nudix hydrolase homolog 10 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020476NUDIX hydrolasePRINTSPR00502NUDIXFAMILYcoord: 72..86
score: 39.81
coord: 86..101
score: 49.14
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 45..188
e-value: 6.4E-26
score: 90.9
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 44..191
score: 20.430998
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 34..197
e-value: 1.5E-52
score: 179.7
NoneNo IPR availablePANTHERPTHR11839:SF22NUDIX HYDROLASE 26, CHLOROPLASTICcoord: 27..197
NoneNo IPR availablePANTHERPTHR11839UDP/ADP-SUGAR PYROPHOSPHATASEcoord: 27..197
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 77..98
IPR022927RNA pyrophosphohydrolase RppHHAMAPMF_00298Nudix_RppHcoord: 39..198
score: 31.434242
IPR022927RNA pyrophosphohydrolase RppHCDDcd03671Ap4A_hydrolase_plant_likecoord: 44..193
e-value: 4.16128E-74
score: 218.229
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 41..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009967.2PI0009967.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006753 nucleoside phosphate metabolic process
biological_process GO:0019693 ribose phosphate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0034432 bis(5'-adenosyl)-pentaphosphatase activity
molecular_function GO:0008893 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity
molecular_function GO:0016787 hydrolase activity