PI0009702 (gene) Melon (PI 482460) v1

Overview


Name	PI0009702
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	homocysteine S-methyltransferase 1
Location	chr10: 20407355 .. 20410277 (+)
RNA-Seq Expression	PI0009702
Synteny	PI0009702

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATTAAGAAGGCGACCACATTGTTGGATGATCTTTTACACAACGCTGGAGGTTGTGCGGTAATCGATGGAGGTTTCGCCACACAGCTTGAGAAACACGGGGCTGTCATTAACGATCCACTTTGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGGTCATCTCTTACAGTATCTATTGTTGTTTCGGTGCATCACCTTCTCATTCTCATCGTTGCTTGGATTGTATGTGCAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGTTTGCAATGCCAATGGACTGACCTTTCTTTGTTTTTGTACTTTTCACTCTGTGTTGGCTTTGGAGTGTCTTTCTTTTAAATATTTTACTCTTGTTTGTTTAAAACTTGATGGTTCAAATTGGATATTTTAATGACGATGAACAGAATGAGTGAGGATCAAACTGATCACTCCTCTTGAATTGAAATCAAAGTTCAAAATGAGCGTTTCTGTCCAGTCGTGAAATGATGTACGAAATTTGACCCGCTGTTAAATGAACAGGATCAAATTGGAGAAATTTCAATCAAGTTAAATGAATTGGATTGAAGTTCTTTCCATGAAGTAACATTTCTGGACATTCAAGTTGTTATAATTAGTGGACTTGATGTACAGGAAGCGACAGTATTTAATATCTGGAAGGCTAATGTTTGTGTGCTTAATAATATCTAATCACATCGGTTTAGCTCCATTGTCAAAAGTGCGTGGTAAATCAAGATCCCTATTCCAAGTGCATCATTACCTATGTAAGATATTAAATATCCTGTAAGTTTTCCAATGTTAAACGTTGCAGAAGTAAGCGATCAGTTCAACAGATTGATTGAATGGCATTTAAAATGATATTTTGTTTGTCATGCTAATGTAAAAGATCTTCATTTTGCCATTTAAAATGAAATTCAATTTTGTATAACTCAGAATTTGTATATATCAATATATTGAATTTATATCCCCTTTTCAGTTTTCATAACTTTATTGTTCCATATCAGAATTTTCTTTATTTTATTGACCCCAGATAGAATTTGAGTGTAAAATATGACATCAGTTTGCGGCTTATAGTTTCTTAAGAATTCCAATAATCTTTAGGAATTGAAATGTTTTCTGGCTTTATTTCTCATAAACTTTTTATAGAAATAATTGCTTCTTTAGAAATTATAAACAGAATACCAATAAATCAGTATTCAAGCCAGTTAATTATTCTCTCTTGTTGAACATTTTATTCTATTACAGGCCACAATTCCTGGGTTTATATCAAAGGGGCTGTCCGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATTGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCACAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTACAGGTGAATTAGAAATAATTACTCACAAGGATTTTTTAAATATTTGGTATTGACAATCTGATCTAAACCTAACCTATTTTCACCCCCCTCCCCAGTGGGCATTATGGGCCAGATGTAAATGTAGGTAAGCTGAAGGATTTTCATCGACGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTGGAAGCCCAGGTTTGTTTTATGCCCCTCTTATATCATCTAAATTGTGGTTTAATGATAGACCCACTCTTACCGCTGCAGTGATGATACCACTGTAAATTATGTTGTTATGCTTTGGTCATAAAGTATTTCAGCAAACAGCTTACCATATGTTTTATAGTCTGTTCTGAATTTATAAGTTGTTCATTTCAAGGGATTGAGGTAATTTGATGACCATCTTCCGTGAATGGGGAGTCCATGGATCATTAGGTCTTAGTTTATGAACTGCATAATTCATGATCAAAATTGTTTTATATTAAAATTATGATTTCTTTTCAAAATTATAATAATAATATATATTTTTAGTTAAATCCTGTTCAAGGCCCTTACTTTAAAAATAATCCCACTTTACACTACCAGATATTCTATTTTAATTCCTAAACCAGAATCCTGATTTAGTTAGTTTTAGTTACAAAATGGAAGAGAAATGTTGAATGTTTCTGGTTATTGTCATGAGCATGCCAGGACACTTGTTTCAATTGTTTTTGCATTTTGCTTGTTTTTCTGTCCTCTCTATGATCATGCTTCCAACCAATCTTACCTTCCGCCCCCCCATTCTGGTCTAAAAAATGTAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACATCAAAATTCCATCCTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCGAGAAGTGCCTCTATGCGATAAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGGTAGGAACTAAATCTCTTATAGTTGAGATTTGATATTGTTTCTTTCATTCAATGGTTTTGAAACGAAATTGTTCGCTACAGTTAACAAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGAAGATCCAAGAAATGGCTGGTGAGTCAACTTATTCCTCCCTCCACCACTCTGATATTTCATGGTTTGTGGGCCTACATTATGATATTTCCATTTCTAATTTAGTGTTCCGGTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTCGAATCATTTAGTTCAAGATGGCGCAATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACGCCGTCTACCATCCGAGCTGTATCAAACGTCTTGAAAGAAAGCTCTCATTAG

mRNA sequence

ATGGGGATTAAGAAGGCGACCACATTGTTGGATGATCTTTTACACAACGCTGGAGGTTGTGCGGTAATCGATGGAGGTTTCGCCACACAGCTTGAGAAACACGGGGCTGTCATTAACGATCCACTTTGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACAATTCCTGGGTTTATATCAAAGGGGCTGTCCGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATTGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCACAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTACAGTGGGCATTATGGGCCAGATGTAAATGTAGGTAAGCTGAAGGATTTTCATCGACGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTGGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACATCAAAATTCCATCCTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCGAGAAGTGCCTCTATGCGATAAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGTTAACAAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGAAGATCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTCGAATCATTTAGTTCAAGATGGCGCAATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACGCCGTCTACCATCCGAGCTGTATCAAACGTCTTGAAAGAAAGCTCTCATTAG

Coding sequence (CDS)

ATGGGGATTAAGAAGGCGACCACATTGTTGGATGATCTTTTACACAACGCTGGAGGTTGTGCGGTAATCGATGGAGGTTTCGCCACACAGCTTGAGAAACACGGGGCTGTCATTAACGATCCACTTTGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACAATTCCTGGGTTTATATCAAAGGGGCTGTCCGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATTGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCACAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTACAGTGGGCATTATGGGCCAGATGTAAATGTAGGTAAGCTGAAGGATTTTCATCGACGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTGGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACATCAAAATTCCATCCTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCGAGAAGTGCCTCTATGCGATAAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGTTAACAAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGAAGATCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTCGAATCATTTAGTTCAAGATGGCGCAATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACGCCGTCTACCATCCGAGCTGTATCAAACGTCTTGAAAGAAAGCTCTCATTAG

Protein sequence

MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNLGATFIGGCCRTTPSTIRAVSNVLKESSH

Homology

[Close]

BLAST of PI0009702 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.6e-148
Identity = 242/324 (74.69%), Postives = 285/324 (87.96%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MG++K + LL+DL+   GGCAV+DGGFATQLE HGA INDPLWSAV LI DP+LIK+VH+
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGAD++V+SSYQATIPGF+S+GLS+EE E LL+KSVKLA+EARD FWD V    GH
Sbjct: 61  EYLEAGADVVVTSSYQATIPGFLSRGLSMEESESLLQKSVKLAVEARDRFWDKVSKTSGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG YG DV++ KLKDFHRRR+Q+LV+ASPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IP+WICF+SVDGENAPSGESF++CL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           C PP F++ LI KF +LT K IVVYPNSGEVWDG++KKWLPS CFGD +FE F+++WR+L
Sbjct: 241 CAPPQFMDNLIRKFSKLTQKAIVVYPNSGEVWDGKAKKWLPSQCFGDAEFEMFATKWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLK 325
           GA  IGGCCRTTPSTI+A+S  LK
Sbjct: 301 GAKLIGGCCRTTPSTIKAISRDLK 324

[Close]

BLAST of PI0009702 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-146
Identity = 240/324 (74.07%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M ++K + LL+DL+   GGCAV+DGGFATQLE HGA INDPLWSAV LI +P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E LL+KSV+LA+EARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYG +V++ KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE ++IP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG++K+WLPS CFGDD+FE F+++WR+L
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLK 325
           GA  IGGCCRTTPSTI A+S  LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of PI0009702 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-127
Identity = 216/316 (68.35%), Postives = 252/316 (79.75%), Query Frame = 0


Query: 9   LLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYLEAGAD 68
           +L+DL+  AGGCAVIDGGFATQLE  GA INDPLWSA CLI  P L+K+VH++YLEAGAD
Sbjct: 3   VLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVKEVHMQYLEAGAD 62

Query: 69  ILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVA 128
           +++SSSYQATIPGFI++G+SV E E LL  SVKLA EARD FW S        YNRALVA
Sbjct: 63  VIISSSYQATIPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRALVA 122

Query: 129 ASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQ 188
           ASIGSYGAYLADGSEYSG YG D+   KLKDFHRRRLQ+L  A PDL+AFE IPN++EAQ
Sbjct: 123 ASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQ 182

Query: 189 ACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIE 248
           A VELLEEE ++IPSWICFSSVDG+N  SGESF  CL  +N S+KV  VG+NCTPP FIE
Sbjct: 183 ALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQFIE 242

Query: 249 ALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNLGATFIGGC 308
            +I +F++ T K I VYPNSGEVWDGR+K+WLP  C G   F++ + RW+  GA+ IGGC
Sbjct: 243 GIICEFRKQTKKAIAVYPNSGEVWDGRAKRWLPVECLGHKSFDALAKRWQEAGASLIGGC 302

Query: 309 CRTTPSTIRAVSNVLK 325
           CRTTPSTIRAVS +LK
Sbjct: 303 CRTTPSTIRAVSKILK 318

[Close]

BLAST of PI0009702 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 5.9e-103
Identity = 181/330 (54.85%), Postives = 238/330 (72.12%), Query Frame = 0


Query: 4   KKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYL 63
           ++ ++L+ D L   GG AV+DGGFAT+L++HGA INDPLWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSF--------WDSVK 123
           E+GA+I++++SYQATI GF++KGLSV E E LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 124 SIPGHKYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDL 183
           +    +    LVAAS+GSYGAYLADGSEYSG YG  V+   LKDFHRRR+QIL  +  DL
Sbjct: 127 AGKASR-RPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADL 186

Query: 184 LAFETIPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVN 243
           +AFETIPNKLEA+A  +LLEEE+I IP+W  F+S DG + P G+S  +C    +    V 
Sbjct: 187 IAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVV 246

Query: 244 AVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSS 303
           A+GINCT P +I ALI   +++T K IVVYPNSGEV+DG +KKW+ S    ++ F S+ S
Sbjct: 247 AIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVS 306

Query: 304 RWRNLGATFIGGCCRTTPSTIRAVSNVLKE 326
           +WR+ GA+  GGCCRTTP+TIRA++ VL +
Sbjct: 307 KWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

[Close]

BLAST of PI0009702 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-101
Identity = 177/315 (56.19%), Postives = 229/315 (72.70%), Query Frame = 0


Query: 10  LDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYLEAGADI 69
           + D L   GG AVIDGG AT+ E+HGA +NDPLWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH-----KYNR 129
           + S+SYQATI GF +KG S EE E LL+KSV++A EAR+S++D   +         K   
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + + KLKDFHRRRLQ+L ++  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPP 249
           +EAQA  +LLEE ++KIP W  F+S DG N  SG+S ++C+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNLGATF 309
            FIE L+ + +++T+K I+VYPNSGE +D   K+W+ +   GD+ F S+  +W + G + 
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSTIRAV 320
           +GGCCRTTP+TIRA+
Sbjct: 309 LGGCCRTTPTTIRAI 323

[Close]

BLAST of PI0009702 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.2e-184
Identity = 317/327 (96.94%), Postives = 319/327 (97.55%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MGIKKATTLLDD LHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVK IPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
           KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNV KLKDFHRRRLQI VDASPDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENI+IPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWLPSNCFGDDKFES SSRWRNL
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESLSSRWRNL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESS 328
           GATFIGGCCRTTPSTIRAVS VLKES+
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKEST 327

[Close]

BLAST of PI0009702 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.0e-183
Identity = 313/328 (95.43%), Postives = 319/328 (97.26%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MGI+KATTLLDDLLHNAGGCAVIDGG ATQLEKHGAV NDPLWSAVCLINDPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILV+SSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVK IPGH
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDVNV KLKDFHRRRL+ILVDASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWLPSNCFGDDKFESF+SRW  L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESFTSRWCKL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GATFIGGCCRTTPSTIRAVS VLKESSH
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKESSH 328

[Close]

BLAST of PI0009702 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.6e-174
Identity = 298/327 (91.13%), Postives = 315/327 (96.33%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M +KKATTLLDDL+ NAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLI DPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGF+SKGLS EEGELLLEKSVKLA+EARDSFW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
           KYNRALVAASIGSYGAYLADGSEYSG+YGPDVNV KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDG+NAPSGESFE+CL AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR+KKWLPSNCFGDDKFESFSSRWR+L
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESS 328
           GA FIGGCCRTTPST RAVS VLKESS
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of PI0009702 vs. ExPASy TrEMBL
Match: A0A6J1G1A5 (homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.8e-171
Identity = 294/328 (89.63%), Postives = 308/328 (93.90%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MG+KKATTLLDDL+ NAGGC VIDGG ATQLEKHGAV ND LWSAVCL+ DPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAG+DILVSSSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVKS PGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDV+V  LK FHRRRLQILV ASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR+KKWLPSNCFGDDKFES SSRWR+L
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GA FIGGCCRTTPSTIRAVS VLKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of PI0009702 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.1e-171
Identity = 294/328 (89.63%), Postives = 308/328 (93.90%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MG+KKA TLLDDL+ NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+ DPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDV+V  LKDFHRRRLQILV ASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++I SWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR+KKWLPSNCFGDDKFES S+RWR+L
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GA FIGGCCRTTPSTIRAVS VLKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of PI0009702 vs. NCBI nr
Match: XP_038893227.1 (homocysteine S-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 316/328 (96.34%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M +KKATTLLDDLL NAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLLQNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYL+AGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARD FWDSVKSIPGH
Sbjct: 61  EYLKAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDGFWDSVKSIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
           KY+RALVAASIGSYGAYLADGSEYSGHYGPDVNV KLKDFHRRRLQILVDASPDLLAFET
Sbjct: 121 KYHRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENI+IPSWICFSS+DGENAPSGESFEKCL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSIDGENAPSGESFEKCLHAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GATFIGGCCRTTPSTIRA+S VLKESSH
Sbjct: 301 GATFIGGCCRTTPSTIRAISKVLKESSH 328

[Close]

BLAST of PI0009702 vs. NCBI nr
Match: XP_004135092.1 (homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypothetical protein Csa_008676 [Cucumis sativus])

HSP 1 Score: 654.1 bits (1686), Expect = 6.6e-184
Identity = 317/327 (96.94%), Postives = 319/327 (97.55%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MGIKKATTLLDD LHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVK IPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
           KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNV KLKDFHRRRLQI VDASPDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEENI+IPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWLPSNCFGDDKFES SSRWRNL
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESLSSRWRNL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESS 328
           GATFIGGCCRTTPSTIRAVS VLKES+
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKEST 327

[Close]

BLAST of PI0009702 vs. NCBI nr
Match: XP_008446599.1 (PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 313/328 (95.43%), Postives = 319/328 (97.26%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MGI+KATTLLDDLLHNAGGCAVIDGG ATQLEKHGAV NDPLWSAVCLINDPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILV+SSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVK IPGH
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDVNV KLKDFHRRRL+ILVDASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWLPSNCFGDDKFESF+SRW  L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESFTSRWCKL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GATFIGGCCRTTPSTIRAVS VLKESSH
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKESSH 328

[Close]

BLAST of PI0009702 vs. NCBI nr
Match: XP_022150665.1 (homocysteine S-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 298/327 (91.13%), Postives = 315/327 (96.33%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M +KKATTLLDDL+ NAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLI DPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGF+SKGLS EEGELLLEKSVKLA+EARDSFW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
           KYNRALVAASIGSYGAYLADGSEYSG+YGPDVNV KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDG+NAPSGESFE+CL AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR+KKWLPSNCFGDDKFESFSSRWR+L
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESS 328
           GA FIGGCCRTTPST RAVS VLKESS
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of PI0009702 vs. NCBI nr
Match: XP_023541078.1 (homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.2 bits (1593), Expect = 4.0e-173
Identity = 297/328 (90.55%), Postives = 310/328 (94.51%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           MG+KKATTLLDDL+ NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+ DPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDV+V  LKDFHRRRLQILV ASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEEN++IPSWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR+KKWLPSNCFGDDKFES SSRWR+L
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLKESSH 329
           GA FIGGCCRTTPSTIRAVS VLKES H
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of PI0009702 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 8.1e-148
Identity = 240/324 (74.07%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M ++K + LL+DL+   GGCAV+DGGFATQLE HGA INDPLWSAV LI +P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E LL+KSV+LA+EARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYG +V++ KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE ++IP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
           C PP FIE LI KF +LT K IVVYPNSGEVWDG++K+WLPS CFGDD+FE F+++WR+L
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLK 325
           GA  IGGCCRTTPSTI A+S  LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of PI0009702 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.1e-135
Identity = 226/324 (69.75%), Postives = 268/324 (82.72%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M ++K + LL+DL+   GGCAV+DGGFATQLE HGA INDPLWSAV LI +P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E LL+KSV+LA+EARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYG +V++ KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE ++IP+WICF+SVDGE APSGESFE+CL  +NKS+ + A    
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA---- 240

Query: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNL 300
                           LT K IVVYPNSGEVWDG++K+WLPS CFGDD+FE F+++WR+L
Sbjct: 241 ----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 301 GATFIGGCCRTTPSTIRAVSNVLK 325
           GA  IGGCCRTTPSTI A+S  LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 304

[Close]

BLAST of PI0009702 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.0e-114
Identity = 191/260 (73.46%), Postives = 227/260 (87.31%), Query Frame = 0


Query: 1   MGIKKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHL 60
           M ++K + LL+DL+   GGCAV+DGGFATQLE HGA INDPLWSAV LI +P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E LL+KSV+LA+EARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYG +V++ KLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240
           IPNKLEAQACVELLEEE ++IP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALITKFKELTNK 261
           C PP FIE LI KF + T++
Sbjct: 241 CAPPQFIENLIRKFAKATDE 260

[Close]

BLAST of PI0009702 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 375.6 bits (963), Expect = 4.2e-104
Identity = 181/330 (54.85%), Postives = 238/330 (72.12%), Query Frame = 0


Query: 4   KKATTLLDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYL 63
           ++ ++L+ D L   GG AV+DGGFAT+L++HGA INDPLWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSF--------WDSVK 123
           E+GA+I++++SYQATI GF++KGLSV E E LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 124 SIPGHKYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDL 183
           +    +    LVAAS+GSYGAYLADGSEYSG YG  V+   LKDFHRRR+QIL  +  DL
Sbjct: 127 AGKASR-RPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADL 186

Query: 184 LAFETIPNKLEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVN 243
           +AFETIPNKLEA+A  +LLEEE+I IP+W  F+S DG + P G+S  +C    +    V 
Sbjct: 187 IAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVV 246

Query: 244 AVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSS 303
           A+GINCT P +I ALI   +++T K IVVYPNSGEV+DG +KKW+ S    ++ F S+ S
Sbjct: 247 AIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVS 306

Query: 304 RWRNLGATFIGGCCRTTPSTIRAVSNVLKE 326
           +WR+ GA+  GGCCRTTP+TIRA++ VL +
Sbjct: 307 KWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

[Close]

BLAST of PI0009702 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 371.3 bits (952), Expect = 7.9e-103
Identity = 177/315 (56.19%), Postives = 229/315 (72.70%), Query Frame = 0


Query: 10  LDDLLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKKVHLEYLEAGADI 69
           + D L   GG AVIDGG AT+ E+HGA +NDPLWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGH-----KYNR 129
           + S+SYQATI GF +KG S EE E LL+KSV++A EAR+S++D   +         K   
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVGKLKDFHRRRLQILVDASPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + + KLKDFHRRRLQ+L ++  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LEAQACVELLEEENIKIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPP 249
           +EAQA  +LLEE ++KIP W  F+S DG N  SG+S ++C+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLPSNCFGDDKFESFSSRWRNLGATF 309
            FIE L+ + +++T+K I+VYPNSGE +D   K+W+ +   GD+ F S+  +W + G + 
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 310 IGGCCRTTPSTIRAV 320
           +GGCCRTTP+TIRA+
Sbjct: 309 LGGCCRTTPTTIRAI 323

The following BLAST results are available for this feature:

BLAST of PI0009702 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4ZGQ8	1.6e-148	74.69	Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN...	[more]
Q9SDL7	1.1e-146	74.07	Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1...	[more]
Q9FUN0	1.2e-127	68.35	Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1	[more]
Q8LAX0	5.9e-103	54.85	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ...	[more]
Q9M1W4	1.1e-101	56.19	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1...	[more]

BLAST of PI0009702 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KR42	3.2e-184	96.94	Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660...	[more]
A0A1S3BEY1	6.0e-183	95.43	homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ...	[more]
A0A6J1D951	6.6e-174	91.13	homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874...	[more]
A0A6J1G1A5	1.8e-171	89.63	homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762...	[more]
A0A6J1HZT4	3.1e-171	89.63	homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]

BLAST of PI0009702 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893227.1	2.3e-184	96.34	homocysteine S-methyltransferase 1 [Benincasa hispida]	[more]
XP_004135092.1	6.6e-184	96.94	homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypo...	[more]
XP_008446599.1	1.2e-182	95.43	PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo]	[more]
XP_022150665.1	1.4e-173	91.13	homocysteine S-methyltransferase 1 [Momordica charantia]	[more]
XP_023541078.1	4.0e-173	90.55	homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0009702 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25900.1	8.1e-148	74.07	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.3	2.1e-135	69.75	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.2	2.0e-114	73.46	Homocysteine S-methyltransferase family protein	[more]
AT3G22740.1	4.2e-104	54.85	homocysteine S-methyltransferase 3	[more]
AT3G63250.1	7.9e-103	56.19	homocysteine methyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 22..322 e-value: 1.7E-72 score: 244.6
IPR003726	Homocysteine-binding domain	PROSITE	PS50970	HCY	coord: 9..323 score: 47.154011
IPR036589	Homocysteine-binding domain superfamily	GENE3D	3.20.20.330		coord: 10..321 e-value: 6.9E-97 score: 326.5
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 17..325
IPR017226	Betaine-homocysteine S-methyltransferase, BHMT	PIRSF	PIRSF037505	BHMT	coord: 2..327 e-value: 3.8E-12 score: 44.1
None	No IPR available	PIRSR	PIRSR037505-2	PIRSR037505-2	coord: 13..242 e-value: 9.4E-11 score: 39.1
None	No IPR available	PANTHER	PTHR46015:SF7	HOMOCYSTEINE S-METHYLTRANSFERASE 1	coord: 1..326
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 1..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0009702.1	PI0009702.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0033528	S-methylmethionine cycle
molecular_function	GO:0047150	betaine-homocysteine S-methyltransferase activity
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0008270	zinc ion binding