PI0009577 (gene) Melon (PI 482460) v1

Overview


Name	PI0009577
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	FSH1 domain-containing protein
Location	chr03: 2746754 .. 2749096 (+)
RNA-Seq Expression	PI0009577
Synteny	PI0009577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCCGGTGAAGAGAAAGACGAAAGGATGAATGCCCTACAAATTGCTTTTTAAATGTCTTCATTCTTCCCCTCCTTCTCTTAACATCCATTTCTCACAACAATTCCAGATTTTCATTTTCAGACAATTCCAATCATGGGAACCGATGCTAAAACCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCCTTCTGTCCTCGATCAACTCGATCTCGATTTCCTCGACGCTCCCTTTCCCGCCGAAGGAAAGTCCGACGTCCAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGTACGATTGCTTCGCCTAATTTCGATTTCCTTGTAAATTCATAAACCTCTCCCTTAATTTCTTTATTTTTGTCCCTTTTTCCATTACAGGAGTTCACAGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGTAATTTCATGTTCCTCTATTCTTTTCCTAATTACTTAAACGGATCCTAATCGAGTGTCAAATTGTGGTTGAATTTCGCTATTTCAGGGGGCCATATTATCCGCGGCTCTGCCGGGATTTCAAGCTAAGGTAACCTAATTAACAACAGCCTGCTCTGTTCTGAGCTATTACAAATGTTTCATTTGCATTCACCAAAATTGAGATTCTAACGCGGCAGTGCCGTTGATTAATGGTTGCAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGACAAAGCTTATTCGACGCCAATTGGATGCCCATCCCTCCATTTTCTAGGTACCTCCACATGATTTTTCTGTCCTTACCCTCTGCTTTTTCAAATATTCTGATTCTTTCCTTTGTTTTTTTTCAAAACAAACAAACCACTCAACAAAATTTGTCATTAATCTACAATTTTCTACAGTATATTTAGTTTCGAAGTAACATAACAGCTTGCCCTAACCAATTGAATTAGGTTCAATTTGATAAATTTTCTAATTAAATAATTATAGGCATTGAGTAATCAAACCAATTGTCGTTGAAATTATAATTAGTATTTTGCCAATTTAGTTTATGATTAGATTGATTCTGCAGATTGAAACGTAATTATAATTAACATTTAATTTTGAAGGATATTTCTTTTATGGTACCAATTGACTATTTCTTTTCTACAAGATTGTTGCAATCAAAATCTTAATTTTTTTATAAATGAATTAATTTTACTATATTTGTCAATATTTTCACATAAAACCATACTTACTCTATTCATGCATAAAACTTCTTGAATGCAATGATTGAGAAAAAGAAACATGAATCAAATTAAATGTGATTGGTTTGTCAACCATATAAGTTGTTTTCATTTTCTACCTTTATTTATTTATATTTATAAATAAGTTACTCAATAATCCTTTTTCCAATCTTATAGGATGATAGGAAAAAAGAATGATTTTATTAAAGTGAATTTGAGCTAGTTTTAAATGGAATTAAGTCCATGAAGAATCTAAAAAAGCTAATTATTTTTTAATGGTCCTGGCTGAACATGGGGGAGTATGGAAAAGTTGTTAAAAAGCGTGAAATCACATTTATTATTTAATTTAAGATTCCATTATTTGATGTTTATTTTATTTTTATTAAAGCTATATGAAATGTTTAATTACAACTTTCATTCTAAAATTATAATTAAAATTTGAATTATTTAATCAACTTATAAAACTTTTGCTTAAAGAAATTGTTAAAATAATGGTGGCAGGTGAGAAGACTTCTTAATGCCACATGGGAAGAAGCTTCTGGAATCATATGTAGAACCAACAGTGATTACTCATCCTAAAGGCCATACAATACCAAGACTAGGTAATACAAAATTTAAACCAATTTCACTTTTGCTTAAATATATATTACTTAATTTCTGATGCTAATTTAGATTCTATCGATTTTATTTATATTTTTAGATGATAAAGCTTTGGAGGTTATGGAGAGTTTCATTCAGAGGATTTCAAAGATTTTAAATGAGACTGAAGAATAGATGTCTGGAGTCTCAATTATGCATTTCTGAACTCATAGAAAAATAAAAAAAGAATTATAATTATTATATGATAATTTTGTAATATAATCTTCATTTTTATGGCAAGATATGTAATAATTAAAAAAAAAAAAAATCAAGATGTAGAGACAATTTTAATCTCCCAATTTATTGTACTTTCAACAAAGTTATTGGTAGTTTAACTTGG

mRNA sequence

TTCCCGGTGAAGAGAAAGACGAAAGGATGAATGCCCTACAAATTGCTTTTTAAATGTCTTCATTCTTCCCCTCCTTCTCTTAACATCCATTTCTCACAACAATTCCAGATTTTCATTTTCAGACAATTCCAATCATGGGAACCGATGCTAAAACCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCCTTCTGTCCTCGATCAACTCGATCTCGATTTCCTCGACGCTCCCTTTCCCGCCGAAGGAAAGTCCGACGTCCAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGAGTTCACAGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCCATATTATCCGCGGCTCTGCCGGGATTTCAAGCTAAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGACAAAGCTTATTCGACGCCAATTGGATGCCCATCCCTCCATTTTCTAGGTGAGAAGACTTCTTAATGCCACATGGGAAGAAGCTTCTGGAATCATATGTAGAACCAACAGTGATTACTCATCCTAAAGGCCATACAATACCAAGACTAGATGATAAAGCTTTGGAGGTTATGGAGAGTTTCATTCAGAGGATTTCAAAGATTTTAAATGAGACTGAAGAATAGATGTCTGGAGTCTCAATTATGCATTTCTGAACTCATAGAAAAATAAAAAAAGAATTATAATTATTATATGATAATTTTGTAATATAATCTTCATTTTTATGGCAAGATATGTAATAATTAAAAAAAAAAAAAATCAAGATGTAGAGACAATTTTAATCTCCCAATTTATTGTACTTTCAACAAAGTTATTGGTAGTTTAACTTGG

Coding sequence (CDS)

ATGGGAACCGATGCTAAAACCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCCTTCTGTCCTCGATCAACTCGATCTCGATTTCCTCGACGCTCCCTTTCCCGCCGAAGGAAAGTCCGACGTCCAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGAGTTCACAGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCCATATTATCCGCGGCTCTGCCGGGATTTCAAGCTAAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGACAAAGCTTATTCGACGCCAATTGGATGCCCATCCCTCCATTTTCTAGGTGAGAAGACTTCTTAA

Protein sequence

MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEKTS

Homology

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BLAST of PI0009577 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 56/174 (32.18%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 9   TKP-RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSD---------- 68
           +KP + LCLHG+  S  +  K++G     +    +L F   P  A+ ++D          
Sbjct: 2   SKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRL 61

Query: 69  --VQGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAAL 128
             + G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L
Sbjct: 62  AALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAML 121

Query: 129 PGFQAKG---IALTKVPKIKFVIIVSGAKFRSESVA-DKAYSTPIGCPSLHFLG 166
                 G       + P  KFV+ V G  FR+E    D  Y+  +  PSLH  G
Sbjct: 122 AQMLQPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAG 173

[Close]

BLAST of PI0009577 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 59/184 (32.07%), Postives = 85/184 (46.20%), Query Frame = 0


Query: 7   TFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPF----------PAEGKS 66
           T  K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVC 409

Query: 67  D------VQGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIENYMIKHGPFDGFL 126
           +      V   FD P    +     QF P    T+   FD+ L++++    + GPFDG L
Sbjct: 410 NKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGIL 469

Query: 127 GFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHF 168
           GFSQGA ++AA+ G Q +   L      +F ++ SG  F    + +      I CPSLH 
Sbjct: 470 GFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHI 528

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BLAST of PI0009577 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-11
Identity = 55/172 (31.98%), Postives = 75/172 (43.60%), Query Frame = 0


Query: 12  RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFP------AEGKSDVQGIFDP- 71
           R LCL GFR S    +++ G    ++  + +L  L  P P      +EG     G   P 
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 72  --PYFEWFQFS--------PEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAA 131
             P   WF            E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 132 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGE 167
           +      G    + P  +FVI+VSG   R   + +     P+  PSLH  G+
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGD 177

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BLAST of PI0009577 vs. ExPASy Swiss-Prot
Match: Q9D7E3 (Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 55/172 (31.98%), Postives = 75/172 (43.60%), Query Frame = 0


Query: 12  RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPA------EGKSDVQGIFDP- 71
           R LCL GFR S    +++ G    ++  + +L  L  P P       EG     G   P 
Sbjct: 8   RVLCLAGFRQSERGFREKTGALRKTLRGRAELVCLSGPHPVPEAAAPEGSCPDSGPCSPE 67

Query: 72  --PYFEWFQ------FS--PEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAA 131
             P   WF       FS   E T  R   E L  +   +   GPFDG LGFSQGA L+A 
Sbjct: 68  EQPRGWWFSEEEADVFSALEESTVCRGLQEALETVARALDTLGPFDGLLGFSQGAALAAY 127

Query: 132 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGE 167
           +      G    + P  +F+I+VSG  F    + +    +P+  PSLH  G+
Sbjct: 128 VCALGQAGD--PRFPLPRFIILVSG--FCPRGLKEPILQSPMSLPSLHVFGD 175

[Close]

BLAST of PI0009577 vs. ExPASy Swiss-Prot
Match: Q8WZ82 (Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-10
Identity = 53/172 (30.81%), Postives = 74/172 (43.02%), Query Frame = 0


Query: 12  RFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPA------EGKSDVQGIFDP- 71
           R LCL GFR S    +++ G    ++  + +L  L  P P       EG     G   P 
Sbjct: 8   RVLCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVPDPPGPEGARSDFGSCPPE 67

Query: 72  --PYFEWFQ------FS--PEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAA 131
             P   WF       FS   E    R  +E L  +   + + GPFDG LGFSQGA L+A 
Sbjct: 68  EQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAALAAL 127

Query: 132 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGE 167
           +      G    + P  +F+++VSG   R     +     P+  PSLH  G+
Sbjct: 128 VCALGQAGD--PRFPLPRFILLVSGFCPRGIGFKESILQRPLSLPSLHVFGD 177

[Close]

BLAST of PI0009577 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 9.9e-89
Identity = 159/167 (95.21%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 167

[Close]

BLAST of PI0009577 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 9.9e-89
Identity = 159/167 (95.21%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 28  MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 194

[Close]

BLAST of PI0009577 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.4e-88
Identity = 159/167 (95.21%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 28  MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 194

[Close]

BLAST of PI0009577 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.4e-82
Identity = 147/167 (88.02%), Postives = 159/167 (95.21%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MGTD +   KPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPFPAEGKSD++G
Sbjct: 28  MGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGAILSAALPG QA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KG+ALTKVPKIKFVIIVSGAKFRS+SVA+KAYS+ IGCPSLHFLG++
Sbjct: 148 KGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 194

[Close]

BLAST of PI0009577 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.4e-82
Identity = 148/167 (88.62%), Postives = 158/167 (94.61%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MGTD +   KPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPFPAEGKSD++G
Sbjct: 28  MGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGAILSAALPG QA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRS+SVA+KAYS  IGCPSLHFLG++
Sbjct: 148 KGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKE 194

[Close]

BLAST of PI0009577 vs. NCBI nr
Match: KAA0065116.1 (dihydrofolate reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 335.9 bits (860), Expect = 2.0e-88
Identity = 159/167 (95.21%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 167

[Close]

BLAST of PI0009577 vs. NCBI nr
Match: XP_008444882.1 (PREDICTED: dihydrofolate reductase-like [Cucumis melo])

HSP 1 Score: 335.9 bits (860), Expect = 2.0e-88
Identity = 159/167 (95.21%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 28  MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 194

[Close]

BLAST of PI0009577 vs. NCBI nr
Match: KAE8652134.1 (hypothetical protein Csa_022239 [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 1.3e-87
Identity = 159/167 (95.21%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 1   MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 167

[Close]

BLAST of PI0009577 vs. NCBI nr
Match: XP_004152614.1 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 1.3e-87
Identity = 159/167 (95.21%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MG+D K FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAPFPAEGKSDV+G
Sbjct: 28  MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 87

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 147

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGE+
Sbjct: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 194

[Close]

BLAST of PI0009577 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 324.7 bits (831), Expect = 4.7e-85
Identity = 155/167 (92.81%), Postives = 159/167 (95.21%), Query Frame = 0


Query: 1   MGTDAKTFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQG 60
           MGTDAK   KPRFLCLHGFRTSAAILKKQVGKWP SVLDQ DL FLDAPFPAEGKSDV+G
Sbjct: 17  MGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQFDLHFLDAPFPAEGKSDVEG 76

Query: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120
           IFDPPYFEWFQFS EFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 77  IFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQA 136

Query: 121 KGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGEK 168
           KGIALTKVPKIKFVIIVSGAKFRSESVA KAYSTPIGCPSLHFLGE+
Sbjct: 137 KGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPSLHFLGEE 183

[Close]

BLAST of PI0009577 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 241.5 bits (615), Expect = 4.9e-64
Identity = 112/167 (67.07%), Postives = 136/167 (81.44%), Query Frame = 0


Query: 1   MGTDAKTFT-KPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQ 60
           MG++ ++   KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDV+
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQ 120
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MIK GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 AKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLGE 167
           AKGIA  KVPKIKFVII+ GAK +S  +A+ AYS+ +   SLHFLGE
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGE 167

[Close]

BLAST of PI0009577 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 239.2 bits (609), Expect = 2.4e-63
Identity = 111/166 (66.87%), Postives = 135/166 (81.33%), Query Frame = 0


Query: 1   MGTDAKTFT-KPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQ 60
           MG++ ++   KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDV+
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQ 120
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MIK GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 AKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHFLG 166
           AKGIA  KVPKIKFVII+ GAK +S  +A+ AYS+ +   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

[Close]

BLAST of PI0009577 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 1.1e-60
Identity = 104/162 (64.20%), Postives = 129/162 (79.63%), Query Frame = 0


Query: 11  PRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPFPAEGKSDVQGIFDPPYFEWF 70
           PR LCLHGFRTS  IL+  +GKWP ++L  LDLDFLDAPFPA GKSDV+  FDPPY+EW+
Sbjct: 30  PRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYEWY 89

Query: 71  QFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPK 130
           Q +  F EYRNF+ECL++IE+YMIK+GPFDG LGFSQGA L+AA+PG Q +G ALTKVPK
Sbjct: 90  QANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKVPK 149

Query: 131 IKFVIIVSGAK-----FRSESVADKAYSTPIGCPSLHFLGEK 168
           +KF++I+SGAK     F     A  A+S+P+ CPSLHF+GE+
Sbjct: 150 VKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGER 191

[Close]

BLAST of PI0009577 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 77.0 bits (188), Expect = 1.6e-14
Identity = 59/184 (32.07%), Postives = 85/184 (46.20%), Query Frame = 0


Query: 7   TFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLDFLDAPF----------PAEGKS 66
           T  K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVC 409

Query: 67  D------VQGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIENYMIKHGPFDGFL 126
           +      V   FD P    +     QF P    T+   FD+ L++++    + GPFDG L
Sbjct: 410 NKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGIL 469

Query: 127 GFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVADKAYSTPIGCPSLHF 168
           GFSQGA ++AA+ G Q +   L      +F ++ SG  F    + +      I CPSLH 
Sbjct: 470 GFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHI 528

The following BLAST results are available for this feature:

BLAST of PI0009577 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36591	2.2e-13	32.18	Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q94AC1	2.2e-13	32.07	Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=ST...	[more]
Q3SZ07	1.6e-11	31.98	Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1	[more]
Q9D7E3	1.8e-10	31.98	Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1	[more]
Q8WZ82	4.0e-10	30.81	Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1	[more]

BLAST of PI0009577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VFL2	9.9e-89	95.21	Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3BC97	9.9e-89	95.21	dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1	[more]
A0A0A0LNX4	6.4e-88	95.21	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 S...	[more]
A0A6J1K928	2.4e-82	88.02	dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV...	[more]
A0A6J1HF66	2.4e-82	88.62	dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 ...	[more]

BLAST of PI0009577 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065116.1	2.0e-88	95.21	dihydrofolate reductase-like [Cucumis melo var. makuwa]	[more]
XP_008444882.1	2.0e-88	95.21	PREDICTED: dihydrofolate reductase-like [Cucumis melo]	[more]
KAE8652134.1	1.3e-87	95.21	hypothetical protein Csa_022239 [Cucumis sativus]	[more]
XP_004152614.1	1.3e-87	95.21	dihydrofolate reductase [Cucumis sativus]	[more]
XP_038885360.1	4.7e-85	92.81	dihydrofolate reductase-like [Benincasa hispida]	[more]

BLAST of PI0009577 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24380.1	4.9e-64	67.07	INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri...	[more]
AT4G24380.2	2.4e-63	66.87	CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop...	[more]
AT5G65400.1	1.1e-60	64.20	alpha/beta-Hydrolases superfamily protein	[more]
AT1G09280.1	1.6e-14	32.07	CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 7..168 e-value: 3.2E-39 score: 136.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 9..166
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 9..167 e-value: 3.8E-39 score: 134.6
None	No IPR available	PANTHER	PTHR48070:SF5	DIHYDROFOLATE REDUCTASE	coord: 9..167
None	No IPR available	PANTHER	PTHR48070	ESTERASE OVCA2	coord: 9..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0009577.1	PI0009577.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus