PI0009491 (gene) Melon (PI 482460) v1

Overview
NamePI0009491
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionbeta-carotene hydroxylase 2, chloroplastic
Locationchr06: 7454729 .. 7458107 (-)
RNA-Seq ExpressionPI0009491
SyntenyPI0009491
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGAGATGCTCCAACAAAGCTCCTCGTTTTTTCCTGCTCATGGTAACTTTGCTCCCACACTTCAACTGAATAAAAAGAAAACAAAAAAACTTGTGGCCTCTGTTTCTTCAACCTCCATTTTTAAGCCCTCTCTCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCTTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTACTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCCTTTCTTTTTCCACCTTCAGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGGAGAAAAACTCTGCTCACTGTCTGCGTACTCGTTGAGGATCAAAATAGTTCCGGCGAGATAGAGAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCACACGTTTCCCAAAGATTAGCAAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTTCTTCTTCCTCTATCTACCTAGAAACCTTAATTGTTTTGTTTTTCTCATTGGCGCCGTCTTCTTCTGATTGAATATTGAATTGCGGTTGTTTGATTTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCAATTTCCATTCATCTCTGTATTCTCTACTTTTCTTGATGAATTCCATTTGCTTTAATCTGATTCTTCACAATCAATTTGCGATTAATCAGGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTCTGGCACTCTTCCTTATGGCATATGCACGAGGTTTCTAATCCTTCTTCACTATATAATTTCTTGATTTCTGTTTTTGTCATCTGAGGAAATTTTTGTTTACATTAATCAACTCTGAAGCTTAATTTGATACCAAAGAAACAATCAATATCCTCTTCTCTATATAGATTTAACTATCGATCTCTCATGTTCCATATAATTATTTGTACAATAAAACAGAGAAAGTCGTTGAGGCTTTGACATTATTGATCTTGAATCTTGATTATTACGACAGTCGCATCATAAACCAAGAGAAGGACCATTCGAGCTGAACGATGTTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCTTACGGGTTCTTCCATAAAGGACTTGTTCCTGGACTCTGCTTTGGCGCAGTAAATCTCTCAATTTCTCTCTCATTATCTTTGGAGAATCTCAGTTTGTTCATACTCCCAACTGTTTAATGGTTGTTTAATCACTGGGGATTAGTGCTAATAAGAGTGTTAAAATGACGAAAATAGCCTTTATAAAAAAAGAAAATTGTGGTATTGAGAAAGGGTTTGACACGCTGGCGGGTTGTGGCAGGGGCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGGTAAAATTTTTTAATTTCTCTATTTCTATTTACCTTCTATTATTATTTTTACCCACTTATCCTAAAAATTTCTCCAATTTTAGTCATTTTTAAAGTTCAATTTTAGCCTATTTTCCGTCTGATTAGAATCCTGAATTAGTTGCTACAGTCAGTAGTTATTATAGACAATGAAATCCTCTGTGTTTTGAGTGTAAACTTGTCTGGTTTGAGTTATTGAGATTTTTGTAAAGTATATTGGTTTAAATGGGTTAAATCTCCTCTTAATTCATTTCATGGGTTAAATTTGAGATTTATATAAAGTATAATAGGTTGAAATGGGACATTTTGAAACTAAATTTGAACAAAAGTCATGGCACAGGAACCAAAAGTGTTATTTTAATAAGTTAGTTATTTCTTTAAAAAATAATTTATGTTTGTGAGAAAGAAAATACATTTATTATAAATTTAAATCTTAATTTTGATCTCAATTTTGATACTGTCTCTGTTTACTCCCTACTGTGTTGACTTTTCATTAATGACTTTTCATATTTGGATCAGTAGATGTAGATTCTACTAATTATTATATGAAAAATGAGTTAAATAAATTCTTGTAGATGTTCTTATTTCTAAATTTAAGAATACCGAAAAGGTAAAAATATCACGATGAAGTAAACATAGAAATGTAGACTCAATAAATATGGTTTTAAATCAACCACCTATTGGGTATCAGTAATAGTTGAAATAATAACTCAAATAATAATAATTAAAATAATTAGATAGCCATGGAGTTCGAAAAGTTCTACGATGTGACATTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGTTAAAACTACTAACATTTTACCAATATCTTAGAGTAAATCTAAATTTAAAATTGAAGCTTATGATTTTCTTTTTCTCCTTTGCTTTAGCTTCACCATTCAGACAAGTTCAATGGTGTGCCATACGGGCTATTTTTGGGACCCAAGGTAAATTTCAGTTCGTATATTCTTTAAATTTTTTTGGAATTTTAATAAAAAGAAAATTTTGTAATGACAAAATTGTTGAAAATATTAAAAAAACATAGTAAAGTAACAAATTCTACCATAGACACTATCTATACCTATACCTAATCATAATTCTATCAAGGTCCGATAAATATTAAATTTTGATTTATTTTGTTATATTTGAAAATATCCCTATAATAAATAGAATTTTTTCTTTTATTTTTATTTTTTTGTAAATTTAAACGGTGAGTGTGCAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCCAAAATCAATCGATGGTTCTTGATCAACCAATATTATGTGAAAATTAAAAAAGAAAAAAGAAAAAAGAAAAAGAAAGCTTTACCTGAGCAGTAACTTTTTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTTTCACTGTATGAAGCAAAGGAGTAGAAGAATGAGATAGTGAATTCGGTACAAATGTTTCACTCTACTTTTTCTTAATTTCTATACGAGCAAAAAAAAAAATAAAAATAAATAAATAAATAAAATACAAGTCAGCCGAACGAAAGTAAACAACACACTCAGTATTTGTGTAATTTTGAATATTTGACAAATTTAATAAAAATATGTAATTATTTGAGCAAGTAGTGTGAATTACGAATATACAACAAAGTTCTTACA

mRNA sequence

AGGAGATGCTCCAACAAAGCTCCTCGTTTTTTCCTGCTCATGGTAACTTTGCTCCCACACTTCAACTGAATAAAAAGAAAACAAAAAAACTTGTGGCCTCTGTTTCTTCAACCTCCATTTTTAAGCCCTCTCTCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCTTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTACTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCCTTTCTTTTTCCACCTTCAGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGGAGAAAAACTCTGCTCACTGTCTGCGTACTCGTTGAGGATCAAAATAGTTCCGGCGAGATAGAGAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCACACGTTTCCCAAAGATTAGCAAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTCTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAGCTGAACGATGTTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCTTACGGGTTCTTCCATAAAGGACTTGTTCCTGGACTCTGCTTTGGCGCAGGGCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCAGACAAGTTCAATGGTGTGCCATACGGGCTATTTTTGGGACCCAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCCAAAATCAATCGATGGTTCTTGATCAACCAATATTATGTGAAAATTAAAAAAGAAAAAAGAAAAAAGAAAAAGAAAGCTTTACCTGAGCAGTAACTTTTTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTTTCACTGTATGAAGCAAAGGAGTAGAAGAATGAGATAGTGAATTCGGTACAAATGTTTCACTCTACTTTTTCTTAATTTCTATACGAGCAAAAAAAAAAATAAAAATAAATAAATAAATAAAATACAAGTCAGCCGAACGAAAGTAAACAACACACTCAGTATTTGTGTAATTTTGAATATTTGACAAATTTAATAAAAATATGTAATTATTTGAGCAAGTAGTGTGAATTACGAATATACAACAAAGTTCTTACA

Coding sequence (CDS)

ATGCTCCAACAAAGCTCCTCGTTTTTTCCTGCTCATGGTAACTTTGCTCCCACACTTCAACTGAATAAAAAGAAAACAAAAAAACTTGTGGCCTCTGTTTCTTCAACCTCCATTTTTAAGCCCTCTCTCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCTTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTACTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCGAACTCCCTTTCTTTTTCCACCTTCAGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGGAGAAAAACTCTGCTCACTGTCTGCGTACTCGTTGAGGATCAAAATAGTTCCGGCGAGATAGAGAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCACACGTTTCCCAAAGATTAGCAAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTCTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAGCTGAACGATGTTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCTTACGGGTTCTTCCATAAAGGACTTGTTCCTGGACTCTGCTTTGGCGCAGGGCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCAGACAAGTTCAATGGTGTGCCATACGGGCTATTTTTGGGACCCAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCCAAAATCAATCGATGGTTCTTGA

Protein sequence

MLQQSSSFFPAHGNFAPTLQLNKKKTKKLVASVSSTSIFKPSLRRLFLREPSSSSTVFSLFTFFSPMAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEDQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSIDGS
Homology
BLAST of PI0009491 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-123
Identity = 213/278 (76.62%), Postives = 239/278 (85.97%), Query Frame = 0

Query: 91  PKPRTPFLFPPSVFR-NSRFQWKMRRKTLLTVCVLVEDQNSSGEIENLSDEESPIVVPQI 150
           P P TP ++P + F  N     + RR+   TVC ++ED     + E   ++    +  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 151 PSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 210
            +  ++++LARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 211 SLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 270
           +LSVGAAVGMEFWARWAH+ALWH+SLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 271 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLH 330
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY RKVAAAH LH
Sbjct: 207 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 266

Query: 331 HSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKW 368
           HS+KFNGVPYGLFLGPKELEEVGGLEELEKE+NRR ++
Sbjct: 267 HSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRRTRY 304

BLAST of PI0009491 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.7e-117
Identity = 218/303 (71.95%), Postives = 245/303 (80.86%), Query Frame = 0

Query: 72  SAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEDQNSS 131
           S++L PK       SS +S    T  +FPP  F+      + R K  LTVC ++E++   
Sbjct: 23  SSSLTPK-------SSPVSTSTPTLVVFPP--FKLVSKSLRTRSKPRLTVCFVLEEKELR 82

Query: 132 GEIENLSDEESPI--VVPQ------IPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMA 191
           G++   SD++     V  Q        +  ++Q+LARKKSERFTYLVAAVMSSFGITSMA
Sbjct: 83  GKLVVASDDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMA 142

Query: 192 VMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHH 251
           V++VYYRF WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWH+SLWHMHESHH
Sbjct: 143 VLSVYYRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHH 202

Query: 252 KPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGL 311
           KPREGPFELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGL
Sbjct: 203 KPREGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGL 262

Query: 312 VHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINR 367
           VHKRFPVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGGL+ LE EINR
Sbjct: 263 VHKRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINR 316

BLAST of PI0009491 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.5e-115
Identity = 221/317 (69.72%), Postives = 247/317 (77.92%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFL---FPPSVFRNSRFQWKMRRKTLLTVCV 126
           MAAGLS A+  KP H   +SS    + R P     F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 127 LVEDQNSSGEIENLSDEESPIVVPQIPSPHVSQRLA----RKKSERFTYLVAAVMSSFGI 186
           +VE++  +  IEN    ES      I + +++ RLA    RKKSER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 187 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 246
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 247 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 306
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 307 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 366
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 367 EINRRIKWTAPKSIDGS 377
           EI+RRIK     S  GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

BLAST of PI0009491 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.5e-113
Identity = 211/302 (69.87%), Postives = 243/302 (80.46%), Query Frame = 0

Query: 67  MAAGLSA-ALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 126
           MAAGLS  A+  KP +    SS  +  P +  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 127 EDQNSSGEIENLSDEESPIVVPQI-PSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAV 186
           E++  S  +++ +  ES     +I  +  + ++  +KKSERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 187 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 246
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 247 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 306
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 307 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 366
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

BLAST of PI0009491 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 406.8 bits (1044), Expect = 2.7e-112
Identity = 204/285 (71.58%), Postives = 232/285 (81.40%), Query Frame = 0

Query: 96  PFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEDQN----SSG----------EIENLSDEE 155
           P LF   +  N     + RRK  L  C +++D       SG          EIE  ++EE
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 156 SPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIP 215
             + V       ++++ ARKKSERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+P
Sbjct: 92  KSLAV------RLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMP 151

Query: 216 FSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINA 275
           FSEMF TF+L+ GAA+GME+WARWAHRALWH+SLWHMHESHH+PREGPFELND+FAIINA
Sbjct: 152 FSEMFCTFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINA 211

Query: 276 VPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRK 335
           VPAIALLS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYF++
Sbjct: 212 VPAIALLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQR 271

Query: 336 VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 367
           VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG LEELEKE+NRRIK
Sbjct: 272 VAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIK 310

BLAST of PI0009491 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.2e-173
Identity = 299/310 (96.45%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAAGLSAA LPKPFHLFLTSSHLSPKP +PFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
            QNSSG+++NLSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTA KSIDGS
Sbjct: 301 WTARKSIDGS 310

BLAST of PI0009491 vs. ExPASy TrEMBL
Match: A0A1S3AZ37 (beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484113 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.5e-171
Identity = 297/310 (95.81%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAALLPKPFHLFL+SSHLS KP TPFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ +LSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTAPKS  GS
Sbjct: 301 WTAPKSNYGS 310

BLAST of PI0009491 vs. ExPASy TrEMBL
Match: A0A088CIY4 (Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.5e-171
Identity = 297/310 (95.81%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAALLPKPFHLFL+SSHLS KP TPFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ +LSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTAPKS  GS
Sbjct: 301 WTAPKSNYGS 310

BLAST of PI0009491 vs. ExPASy TrEMBL
Match: A0A5A7SUR5 (Beta-carotene hydroxylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001660 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.9e-171
Identity = 296/310 (95.48%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAALLPKPFHLFL+SSHLS KP TPFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ +LSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAA++SSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAILSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTAPKS  GS
Sbjct: 301 WTAPKSNYGS 310

BLAST of PI0009491 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.2e-165
Identity = 289/310 (93.23%), Postives = 297/310 (95.81%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA +S AL P+P HLFL SSHLSPKPRTPFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+E+LSDEE PIVVPQIPSP+V+QRLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
            T  KSIDGS
Sbjct: 301 LTTTKSIDGS 310

BLAST of PI0009491 vs. NCBI nr
Match: XP_004140758.1 (beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothetical protein Csa_009664 [Cucumis sativus])

HSP 1 Score: 618.2 bits (1593), Expect = 4.6e-173
Identity = 299/310 (96.45%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAAGLSAA LPKPFHLFLTSSHLSPKP +PFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
            QNSSG+++NLSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTA KSIDGS
Sbjct: 301 WTARKSIDGS 310

BLAST of PI0009491 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 616.7 bits (1589), Expect = 1.3e-172
Identity = 298/310 (96.13%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAAGLSAAL+PKP HL LTSSHLSPKPR PFLFPPSVFRNSR QWKMRR+TL TVCVLVE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ENLSDE SPI+VPQIPSPHVS+RLARKKSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           W APKSIDGS
Sbjct: 301 WAAPKSIDGS 310

BLAST of PI0009491 vs. NCBI nr
Match: NP_001315399.1 (beta-carotene hydroxylase 2, chloroplastic [Cucumis melo] >AID51467.1 beta-carotene hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 610.9 bits (1574), Expect = 7.3e-171
Identity = 297/310 (95.81%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAALLPKPFHLFL+SSHLS KP TPFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ +LSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTAPKS  GS
Sbjct: 301 WTAPKSNYGS 310

BLAST of PI0009491 vs. NCBI nr
Match: KAA0033696.1 (beta-carotene hydroxylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 296/310 (95.48%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAALLPKPFHLFL+SSHLS KP TPFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQNSSGE+ +LSDEESPIVVPQIPSPHVS+RLARKKSERFTYLVAA++SSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAILSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
           WTAPKS  GS
Sbjct: 301 WTAPKSNYGS 310

BLAST of PI0009491 vs. NCBI nr
Match: KAG7014750.1 (Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.0 bits (1525), Expect = 3.5e-165
Identity = 290/310 (93.55%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 126
           MAA LSAAL PKP HLFL SSHLSPKPRTPFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 127 DQNSSGEIENLSDEESPIVVPQIPSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAVMA 186
           DQN+SGE+E+LSDEE PIVVPQIPSP+V+QRLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNNSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 187 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 246
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 247 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 306
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 307 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 366
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 367 WTAPKSIDGS 377
            T  KSI+GS
Sbjct: 301 LTTTKSINGS 310

BLAST of PI0009491 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 416.4 bits (1069), Expect = 2.5e-116
Identity = 221/317 (69.72%), Postives = 247/317 (77.92%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFL---FPPSVFRNSRFQWKMRRKTLLTVCV 126
           MAAGLS A+  KP H   +SS    + R P     F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 127 LVEDQNSSGEIENLSDEESPIVVPQIPSPHVSQRLA----RKKSERFTYLVAAVMSSFGI 186
           +VE++  +  IEN    ES      I + +++ RLA    RKKSER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 187 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 246
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 247 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 306
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 307 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 366
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 367 EINRRIKWTAPKSIDGS 377
           EI+RRIK     S  GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

BLAST of PI0009491 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 410.2 bits (1053), Expect = 1.8e-114
Identity = 211/302 (69.87%), Postives = 243/302 (80.46%), Query Frame = 0

Query: 67  MAAGLSA-ALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 126
           MAAGLS  A+  KP +    SS  +  P +  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 127 EDQNSSGEIENLSDEESPIVVPQI-PSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAV 186
           E++  S  +++ +  ES     +I  +  + ++  +KKSERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 187 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 246
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 247 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 306
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 307 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 366
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

BLAST of PI0009491 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 260.0 bits (663), Expect = 2.9e-69
Identity = 144/226 (63.72%), Postives = 165/226 (73.01%), Query Frame = 0

Query: 67  MAAGLSAALLPKPFHLFLTSSHLSPKPRTPFL---FPPSVFRNSRFQWKMRRKTLLTVCV 126
           MAAGLS A+  KP H   +SS    + R P     F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 127 LVEDQNSSGEIENLSDEESPIVVPQIPSPHVSQRLA----RKKSERFTYLVAAVMSSFGI 186
           +VE++  +  IEN    ES      I + +++ RLA    RKKSER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 187 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 246
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 247 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 286
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

BLAST of PI0009491 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 248.4 bits (633), Expect = 8.9e-66
Identity = 150/302 (49.67%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 67  MAAGLSA-ALLPKPFHLFLTSSHLSPKPRTPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 126
           MAAGLS  A+  KP +    SS  +  P +  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 127 EDQNSSGEIENLSDEESPIVVPQI-PSPHVSQRLARKKSERFTYLVAAVMSSFGITSMAV 186
           E++  S  +++ +  ES     +I  +  + ++  +KKSERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 187 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 246
           MAVYYRF WQM+GGE+   EMFGTF+LSVGA                             
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGA----------------------------- 180

Query: 247 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 306
                                                   AGLGIT+FGMAYMFVHDGLV
Sbjct: 181 ----------------------------------------AGLGITMFGMAYMFVHDGLV 225

Query: 307 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 366
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498142.0e-12376.62Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=... [more]
B3SGL01.7e-11771.95Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=... [more]
Q9SZZ83.5e-11569.72Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LTG02.5e-11369.87Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498152.7e-11271.58Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0L6062.2e-17396.45Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3AZ373.5e-17195.81beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034841... [more]
A0A088CIY43.5e-17195.81Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV... [more]
A0A5A7SUR57.9e-17195.48Beta-carotene hydroxylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1E4L52.2e-16593.23beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_004140758.14.6e-17396.45beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothe... [more]
XP_038877448.11.3e-17296.13beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida][more]
NP_001315399.17.3e-17195.81beta-carotene hydroxylase 2, chloroplastic [Cucumis melo] >AID51467.1 beta-carot... [more]
KAA0033696.11.6e-17095.48beta-carotene hydroxylase 2 [Cucumis melo var. makuwa][more]
KAG7014750.13.5e-16593.55Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argy... [more]
Match NameE-valueIdentityDescription
AT4G25700.12.5e-11669.72beta-hydroxylase 1 [more]
AT5G52570.11.8e-11469.87beta-carotene hydroxylase 2 [more]
AT4G25700.22.9e-6963.72beta-hydroxylase 1 [more]
AT5G52570.28.9e-6649.67beta-carotene hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 215..342
e-value: 8.7E-14
score: 52.0
NoneNo IPR availablePANTHERPTHR31899:SF10BETA-CAROTENE HYDROXYLASEcoord: 82..368
IPR045019Beta-carotene hydroxylase-likePANTHERPTHR31899BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTICcoord: 82..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009491.1PI0009491.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity