PI0009453 (gene) Melon (PI 482460) v1

Overview


Name	PI0009453
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Type VI secretion protein
Location	chr11: 9482739 .. 9484310 (+)
RNA-Seq Expression	PI0009453
Synteny	PI0009453

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCAGAGACGCTGCATACGCTCTTTGCTGGTGCACAAGGCACGGGGAAATCCCAGCAGTTTTTCGTCGCCTTGATGCAAGAGGTTCGGGCAAGAGGTAAGCCTATGGTCGTCTATGACCCGACCGGAGAGTTTACCCAAGCCTTCTACCGGGAAGGTAAAGACATTCTGATGAACCCGCTGGATGCCCGTAGCCCGAACTAGAATATCTGGGCTGAGATCTCCAAGGACTACCACTTCGATAATATGGCCAAGGGCCTAATCCCAGACCTAGTCTGACCCCTTTTTGGCCTTGGCGGGCCGGAGGGTGCTCAAGGACCTTATCTCCGTTCTAGGCAGAGATGGGCATAAACCGAGATTTCGTTAAGGCCGTTGCCAAAAGCAATCTCGAGGAGATGCACGCCTTACTGAAAGGCACCGCTGGCCTATGTGGACTCGATGACGGATCGCACGGGAATCTCACTCAAAATGACCGTTCAAAATCAGCTTCCATCCTTTTGATTTTGACCGGGGGATCTGTTCTCCATCCGCAAGTGGGTGACCTTAACATAGGTTCTTCCTTTTAATTAATAAGAACCCACGTGAAAAGAATATTGAAAAGAATATTGCGTGTTATGAGAGTTGAATGACAAAGAAAGAATATTGCGTCCGTCTTAATATAAAATAGGTGCAAAGAGCGTTGAGAAGAAGATGAAGACTGGATGTTTATCACTGCGCGAGAGGAGATGCGAGAAGGACTGAAGGGTTTTGTCCTTGTGGATCGACACAGCAATCAAAGCCGTGCTGAATCTGGAGTCGATTCACCAAGAGCGCCTCTGGTTCTGCATCGACGAGCTACCAACCCTACCTCGAGATTTTGAAGCTCGCCTTAAGCAAGACGCGAAAGTACGGCTTGTGCGTGGTACTGGGGGTCCAGGACTTCTCTCAGCTTTACGGGATTTACGGACGTGATCTGGCGAAGACGATTATCTCCGGTTGCCAAACCTACTGCTGCGCGTAAGGCGCATCGGCCAAGCTGCTGGCCGAGCTCCTGGGTCAGGCAGAAGTTAGCGAAAAGGAAGAAAGCTTGAGCTATGGCCCCAGTGCCCATCGTGATGGGGTCAGTCTCTTCACCCACCGTGCCATGCGAGACCTGGTGCTGACCTCTGAGATTTTGACCAGATATGAGGGGCTATTTATTCCAGGAGATTACCTGATTGTGAGAGTTCAGTACGGTTACACGCCCTTTGAGGTTGAGAAAGGGGCTGAGGGATTTGTGGAAGGTGGTGGGCTAGAGGCCGTTTTCAAACCAAGGGGGTCGCTTTCTGTGGGTGAGGTTGAAAGGCCAACCCAGAAGGTAAATTCACTAGAGGATCTTTTGTAAAAGGGTGTCCAGAACGGTACGACTGCTCTCACCCTATTCAAAGAGGAAATCTGATTGATTTATGAGATATAGCGCGATGGATTGGACTATGATTAGAGAGCAAGAGCAGATTGAGCTTAGCGCCTCAATATTCTTTAAACGTCGCTAGCCAGCGAATCGAAGGCTTGGAGCCTGATGCCGAAGCCATGGAGTATGCCGAAAAATAG

mRNA sequence

ATGAAGTCAGAGACGCTGCATACGCTCTTTGCTGGTGCACAAGGCACGGGGAAATCCCAGCAGTTTTTCGTCGCCTTGATGCAAGAGGTTCGGGCAAGAGGTAAGCCTATGGTCGTCTATGACCCGACCGGAGAGTTTACCCAAGCCTTCTACCGGGAAGAGATGGGCATAAACCGAGATTTCGTTAAGGCCGTTGCCAAAAGCAATCTCGAGGAGATGCACGCCTTACTGAAAGGCACCGCTGGCCTATGTGGACTCGATGACGGATCGCACGGGAATCTCACTCAAAATGACCGTTCAAAATCAGCTTCCATCCTTTTGATTTTGACCGGGGGATCTGTTCTCCATCCGCAAGTGGGTGACCTTAACATAGCAATCAAAGCCGTGCTGAATCTGGAGTCGATTCACCAAGAGCGCCTCTGGTTCTGCATCGACGAGCTACCAACCCTACCTCGAGATTTTGAAGCTCGCCTTAAGCAAGACGCGAAAGTACGGCTTGTGCGTGGCGCATCGGCCAAGCTGCTGGCCGAGCTCCTGGGTCAGGCAGAAGTTAGCGAAAAGGAAGAAAGCTTGAGCTATGGCCCCAGTGCCCATCGTGATGGGGTCAGTCTCTTCACCCACCGTGCCATGCGAGACCTGGTGCTGACCTCTGAGATTTTGACCAGATATGAGGGGCTATTTATTCCAGGAGATTACCTGATTGTGAGAGTTCAGTACGGTTACACGCCCTTTGAGGTTGAGAAAGGGGCTGAGGGATTTGTGGAAGGTGGTGGGCTAGAGGCCGTTTTCAAACCAAGGGGGTCGCTTTCTGTGGGTGAGGTTGAAAGGCCAACCCAGAAGCGAATCGAAGGCTTGGAGCCTGATGCCGAAGCCATGGAGTATGCCGAAAAATAG

Coding sequence (CDS)

ATGAAGTCAGAGACGCTGCATACGCTCTTTGCTGGTGCACAAGGCACGGGGAAATCCCAGCAGTTTTTCGTCGCCTTGATGCAAGAGGTTCGGGCAAGAGGTAAGCCTATGGTCGTCTATGACCCGACCGGAGAGTTTACCCAAGCCTTCTACCGGGAAGAGATGGGCATAAACCGAGATTTCGTTAAGGCCGTTGCCAAAAGCAATCTCGAGGAGATGCACGCCTTACTGAAAGGCACCGCTGGCCTATGTGGACTCGATGACGGATCGCACGGGAATCTCACTCAAAATGACCGTTCAAAATCAGCTTCCATCCTTTTGATTTTGACCGGGGGATCTGTTCTCCATCCGCAAGTGGGTGACCTTAACATAGCAATCAAAGCCGTGCTGAATCTGGAGTCGATTCACCAAGAGCGCCTCTGGTTCTGCATCGACGAGCTACCAACCCTACCTCGAGATTTTGAAGCTCGCCTTAAGCAAGACGCGAAAGTACGGCTTGTGCGTGGCGCATCGGCCAAGCTGCTGGCCGAGCTCCTGGGTCAGGCAGAAGTTAGCGAAAAGGAAGAAAGCTTGAGCTATGGCCCCAGTGCCCATCGTGATGGGGTCAGTCTCTTCACCCACCGTGCCATGCGAGACCTGGTGCTGACCTCTGAGATTTTGACCAGATATGAGGGGCTATTTATTCCAGGAGATTACCTGATTGTGAGAGTTCAGTACGGTTACACGCCCTTTGAGGTTGAGAAAGGGGCTGAGGGATTTGTGGAAGGTGGTGGGCTAGAGGCCGTTTTCAAACCAAGGGGGTCGCTTTCTGTGGGTGAGGTTGAAAGGCCAACCCAGAAGCGAATCGAAGGCTTGGAGCCTGATGCCGAAGCCATGGAGTATGCCGAAAAATAG

Protein sequence

MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGINRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGNLTQNDRSKSASILLILTGGSVLHPQVGDLNIAIKAVLNLESIHQERLWFCIDELPTLPRDFEARLKQDAKVRLVRGASAKLLAELLGQAEVSEKEESLSYGPSAHRDGVSLFTHRAMRDLVLTSEILTRYEGLFIPGDYLIVRVQYGYTPFEVEKGAEGFVEGGGLEAVFKPRGSLSVGEVERPTQKRIEGLEPDAEAMEYAEK

Homology

[Close]

BLAST of PI0009453 vs. ExPASy TrEMBL
Match: U3G7M6 (Type IV conjugative transfer system coupling protein TraD OS=Ralstonia sp. 5_2_56FAA OX=658080 GN=HMPREF0989_04420 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 7.4e-55
Identity = 166/419 (39.62%), Postives = 202/419 (48.21%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 278
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. ExPASy TrEMBL
Match: F4GGS9 (Type IV secretion system, TraD, DNA-binding domain protein OS=Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601) OX=596154 GN=Alide2_4751 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 7.4e-55
Identity = 166/419 (39.62%), Postives = 202/419 (48.21%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 278
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. ExPASy TrEMBL
Match: A1WDH0 (TraD OS=Acidovorax sp. (strain JS42) OX=232721 GN=Ajs_4194 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 7.4e-55
Identity = 166/419 (39.62%), Postives = 202/419 (48.21%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 278
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. ExPASy TrEMBL
Match: A0A240UKP4 (Type VI secretion protein OS=Acidovorax carolinensis OX=553814 GN=CBP36_21875 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 9.6e-55
Identity = 162/400 (40.50%), Postives = 195/400 (48.75%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 266
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. ExPASy TrEMBL
Match: A0A7I8HQJ3 (Type IV secretion system DNA-binding domain-containing protein OS=Pseudomonas aeruginosa OX=287 GN=traD PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.1e-54
Identity = 161/400 (40.25%), Postives = 195/400 (48.75%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VR+RGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRSRGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 266
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. NCBI nr
Match: WP_009242071.1 (MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Betaproteobacteria] >MBI2747594.1 type IV secretion system DNA-binding domain-containing protein [Burkholderiales bacterium] >AEB87049.1 Type IV secretion system, TraD, DNA-binding domain protein [Alicycliphilus denitrificans K601] >EFP63592.1 putative type IV conjugative transfer system coupling protein TraD [Ralstonia pickettii] >EGY61034.1 type IV conjugative transfer system coupling protein TraD [Ralstonia sp. 5_2_56FAA] >KAB0466847.1 type IV secretion system DNA-binding domain-containing protein [Ralstonia insidiosa])

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-54
Identity = 166/419 (39.62%), Postives = 202/419 (48.21%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 278
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. NCBI nr
Match: WP_011798637.1 (type IV secretion system DNA-binding domain-containing protein [Acidovorax sp. JS42] >ABM44295.1 TraD [Acidovorax sp. JS42])

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-54
Identity = 166/419 (39.62%), Postives = 202/419 (48.21%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 278
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. NCBI nr
Match: WP_086929254.1 (type IV secretion system DNA-binding domain-containing protein [Acidovorax carolinensis] >ART57385.1 type VI secretion protein [Acidovorax carolinensis] >ART61612.1 type VI secretion protein [Acidovorax carolinensis])

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-54
Identity = 162/400 (40.50%), Postives = 195/400 (48.75%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 266
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. NCBI nr
Match: WP_038314818.1 (type IV secretion system DNA-binding domain-containing protein [Comamonas sp. NyZ500] >MBL5980201.1 type IV secretion system DNA-binding domain-containing protein [Comamonas sp. NyZ500] >BCZ16647.1 hypothetical protein CTYAZ2_49720 [Comamonas testosteroni])

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-54
Identity = 162/400 (40.50%), Postives = 195/400 (48.75%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMANGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 266
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

[Close]

BLAST of PI0009453 vs. NCBI nr
Match: WP_217021595.1 (type IV secretion system DNA-binding domain-containing protein [Burkholderia multivorans] >MBU9516487.1 type IV secretion system DNA-binding domain-containing protein [Burkholderia multivorans])

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-54
Identity = 162/400 (40.50%), Postives = 195/400 (48.75%), Query Frame = 0


Query: 1   MKSETLHTLFAGAQGTGKSQQFFVALMQEVRARGKPMVVYDPTGEFTQAFYREEMGI--- 60
           +KSETLHTLFAGAQGTGKSQQFF ALM++VRARGK M+VYDPTGEFTQAFYRE   I   
Sbjct: 180 VKSETLHTLFAGAQGTGKSQQFF-ALMKQVRARGKRMIVYDPTGEFTQAFYREGKDILMN 239

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 240 PLDSRSPNWNIWNEIAKDYHFDNMASGLIPDPAESDPFWALAGRMVLKDVIAVLGREGRR 299

Query: 121 -NRDFVKAVAKSNLEEMHALLKGTAGLCGLDDGSHGN------LTQN---------DRSK 180
            NRD   A+AKSNL+ MHALL+GTAG   +D  +           QN         D  +
Sbjct: 300 TNRDLYNAIAKSNLDAMHALLQGTAGATYVDPTTERTGMSLKMTVQNQLEAFRFLPDEGE 359

Query: 181 SASI----------LLILTGGSVLHPQVGD-----LNIAIKAVLNLESIHQERLWFCIDE 240
           S SI           + +T    +   +       ++ AIKAVL+LE IH+ERLWFCIDE
Sbjct: 360 SFSIRKWVHEEGDSWMFITAREAMREALKPVLSLWIDTAIKAVLDLEPIHRERLWFCIDE 419

Query: 241 LPTLPR-----------------------DFE---------------ARLKQDAKVRLVR 266
           LPTL +                       DF                +  +    +R+  
Sbjct: 420 LPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYEIYGHDLAKTIISGCQTKLLLRVTD 479

The following BLAST results are available for this feature:

BLAST of PI0009453 vs.


Match Name	E-value	Identity	Description

BLAST of PI0009453 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
U3G7M6	7.4e-55	39.62	Type IV conjugative transfer system coupling protein TraD OS=Ralstonia sp. 5_2_5...	[more]
F4GGS9	7.4e-55	39.62	Type IV secretion system, TraD, DNA-binding domain protein OS=Alicycliphilus den...	[more]
A1WDH0	7.4e-55	39.62	TraD OS=Acidovorax sp. (strain JS42) OX=232721 GN=Ajs_4194 PE=4 SV=1	[more]
A0A240UKP4	9.6e-55	40.50	Type VI secretion protein OS=Acidovorax carolinensis OX=553814 GN=CBP36_21875 PE...	[more]
A0A7I8HQJ3	2.1e-54	40.25	Type IV secretion system DNA-binding domain-containing protein OS=Pseudomonas ae...	[more]

BLAST of PI0009453 vs. NCBI nr


Match Name	E-value	Identity	Description
WP_009242071.1	1.5e-54	39.62	MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Be...	[more]
WP_011798637.1	1.5e-54	39.62	type IV secretion system DNA-binding domain-containing protein [Acidovorax sp. J...	[more]
WP_086929254.1	2.0e-54	40.50	type IV secretion system DNA-binding domain-containing protein [Acidovorax carol...	[more]
WP_038314818.1	2.0e-54	40.50	type IV secretion system DNA-binding domain-containing protein [Comamonas sp. Ny...	[more]
WP_217021595.1	2.0e-54	40.50	type IV secretion system DNA-binding domain-containing protein [Burkholderia mul...	[more]

BLAST of PI0009453 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019476	Type IV secretion system coupling protein TraD, DNA-binding domain	PFAM	PF10412	TrwB_AAD_bind	coord: 122..152 e-value: 7.9E-6 score: 25.0 coord: 168..237 e-value: 1.6E-9 score: 37.1 coord: 2..54 e-value: 1.1E-13 score: 50.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..165 e-value: 2.3E-15 score: 58.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 7..242
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 276..297
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 274..297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0009453.1	PI0009453.1	mRNA